Updated on 2022/11/18


INAGAKI Masahito
Graduate School of Science Designated assistant professor
Designated assistant professor

Research Interests 1

  1. Nucleic Acids Chemistry

Research Areas 1

  1. Life Science / Bioorganic chemistry  / Nucleic Acids Chemistry

Awards 3

  1. 甲南FIBER若い世代の特別講演会特別講演賞

    2022.2   甲南大学先端生命工学研究所   Site-Specific Chemical Strand Cleavage of Oligonucleotides for Genome-Scale DNA Assembly


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    Award type:Award from international society, conference, symposium, etc. 

  2. ISNAC Outstanding Poster Award in 2021

    2021.11   Japan Society of Nucleic Acids Chemistry   Metal Nanoparticles Induced Strand Cleavage of Oligonucleotides for Genome-Scale DNA Synthesis

    Masahito Inagaki

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    Award type:Award from international society, conference, symposium, etc. 

  3. 2020年「貴金属に関わる研究助成金」シルバー賞

    2021.4   一般財団法人 田中貴金属記念財団   貴金属材料を利用したゲノム合成技術の開発と医療創薬応用


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    Award type:Award from publisher, newspaper, foundation, etc. 


Papers 3

  1. Design of Synthetic mRNAs for Highly Efficient Translation

    Inagaki Masahito, Tada Mizuki, Abe Hiroshi

    Drug Delivery System   Vol. 37 ( 3 ) page: 196 - 208   2022.7

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    Language:Japanese   Publisher:THE JAPAN SOCIETY OF DRUG DELIVERY SYSTEM  

    Recently messenger RNA (mRNA) therapeutics is received much attention as one of the vaccination therapies to compete against the coronavirus disease 2019 (COVID-19) pandemic. mRNA therapeutics are generally produced by <i>in vitro</i> transcription utilizing RNA polymerase mediated elongation. However, its purity, stability, and protein synthesis ability, are difficult to be precisely controlled, which is pointed out as drawbacks that must be overcome. To overcome these issues, the introduction of chemically modified nucleic acids is focusing attention. However, it is difficult to flexible molecular design due to the requirement of RNA polymerase recognition ability of chemically modified nucleic acids under <i>in vitro</i> transcription reaction. In the future, the development of a new mRNA design concept based on a flexible molecular design by the progress of chemically modified mRNA therapeutics synthesis method. Under the situation, the authors are focusing on the translation mechanism of mRNA and proposing a new mRNA molecular design to accelerate the translation reaction cycle. In this paper, we introduce an update on therapeutic mRNA design.

    DOI: 10.2745/dds.37.196


    CiNii Research

  2. Complete Chemical Synthesis of Minimal Messenger RNA by Efficient Chemical Capping Reaction

    Abe Naoko, Imaeda Akihiro, Inagaki Masahito, Li Zhenmin, Kawaguchi Daisuke, Onda Kaoru, Nakashima Yuko, Uchida Satoshi, Hashiya Fumitaka, Kimura Yasuaki, Abe Hiroshi

    ACS CHEMICAL BIOLOGY   Vol. 17 ( 6 ) page: 1308 - 1314   2022.6

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    Language:Japanese   Publisher:ACS Chemical Biology  

    Site-specific chemical modification of mRNA can improve its translational efficiency and stability. For this purpose, it is desirable to develop a complete chemical synthesis method for chemically modified mRNA. The key is a chemical reaction that introduces a cap structure into the chemically synthesized RNA. In this study, we developed a fast and quantitative chemical capping reaction between 5′-phosphorylated RNA and N7-methylated GDP imidazolide in the presence of 1-methylimidazole in the organic solvent dimethyl sulfoxide. It enabled quantitative preparation of capping RNA within 3 h. We prepared chemically modified 107-nucleotide mRNAs, including N6-methyladenosine, insertion of non-nucleotide linkers, and 2′-O-methylated nucleotides at the 5′ end and evaluated their effects on translational activity in cultured HeLa cells. The results showed that mRNAs with non-nucleotide linkers in the untranslated regions were sufficiently tolerant to translation and that mRNAs with the Cap_2 structure had higher translational activity than those with the Cap_0 structure.

    DOI: 10.1021/acschembio.1c00996

    Web of Science



  3. N-Benzoyl-protected Peptide Nucleic Acid (PNA) Monomers Expand the Range of Nucleobases Available for PNA-DNA Chimera Reviewed

    Masahito Inagaki, Ryohei Uematsu, Tatsuya Mizutani, Daisuke Unabara, Yasuyuki Araki, Seiji Sakamoto, Hiromu Kashida, Masaki Nishijima, Hiroyuki Asanuma, Yoshihisa Inoue, Takehiko Wada

    Chemistry Letters   Vol. 48 ( 4 ) page: 341 - 344   2019.4

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    Authorship:Lead author   Language:English  

    DOI: 10.1246/cl.181048