Updated on 2022/04/19

写真a

 
TSUNEMATSU Yuta
 
Organization
Graduate School of Bioagricultural Sciences Department of Applied Biosciences Associate professor
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Applied Biosciences
Title
Associate professor
Contact information
メールアドレス
External link

Degree 1

  1. Ph. D. ( 2011.3   Kyoto University ) 

Research Interests 10

  1. きのこ

  2. 冬虫夏草

  3. Synthetic Biology

  4. colorectal cancer

  5. chemistry of natural products

  6. biosynthesis

  7. 生物有機化学

  8. 生薬学

  9. Aspergillus

  10. 腸内細菌

Research Areas 4

  1. Nanotechnology/Materials / Chemical biology

  2. Life Science / Environmental and natural pharmaceutical resources

  3. Nanotechnology/Materials / Chemistry and chemical methodology of biomolecules

  4. Life Science / Bioorganic chemistry

Research History 4

  1. Nagoya University   Graduate School of Bioagricultural Sciences   Associate professor

    2022.4

  2. FY2021, FOREST (Fusion Oriented REsearch for disruptive Science and Technology) program, JST

    2022.4

  3. A start-up company in the USA   Consultant

    2021.4 - 2022.3

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    Country:United States

  4. University of Shizuoka   Department of Pharmaceutical Sciences   Assistant Professor

    2017.7 - 2022.3

Professional Memberships 6

  1. SOCIETY FOR ACTINOMYCETES JAPAN

  2. The Society for Biotechnology, Japan

  3. THE JAPANESE SOCIETY OF PHARMACOGNOSY

  4. THE PHARMACEUTICAL SOCIETY OF JAPAN

  5. JAPAN SOCIETY FOR BIOSCIENCE, BIOTECHNOLOGY, AND AGROCHEMISTRY

  6. 糸状菌分子生物学研究会

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Committee Memberships 2

  1. 天然物化学談話会   世話人  

    2017.4   

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    Committee type:Academic society

  2. 静岡県立大学薬学部   研究倫理審査部局委員  

    2017.4 - 2022.3   

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    Committee type:Academic society

 

Papers 11

  1. Genomics-directed activation of cryptic natural product pathways deciphers codes for biosynthesis and molecular function.

    Tsunematsu Y

    Journal of natural medicines   Vol. 75 ( 2 ) page: 261 - 274   2021.3

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    Language:English  

    DOI: 10.1007/s11418-020-01466-x

    PubMed

  2. Specialized Flavoprotein Promotes Sulfur Migration and Spiroaminal Formation in Aspirochlorine Biosynthesis. Reviewed International journal

    Yuta Tsunematsu, Naoya Maeda, Michio Sato, Kodai Hara, Hiroshi Hashimoto, Kenji Watanabe, Christian Hertweck

    Journal of the American Chemical Society   Vol. 143 ( 1 ) page: 206 - 213   2021.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    Epidithiodiketopiperazines (ETPs) are a class of ecologically and medicinally important cyclodipeptides bearing a reactive transannular disulfide bridge. Aspirochlorine, an antifungal and toxic ETP isolated from Aspergillus oryzae used in sake brewing, deviates from the common ETP scaffold owing to its unusual ring-enlarged disulfide bridge linked to a spiroaminal ring system. Although this disulfide ring system is implicated in the biological activity of ETPs the biochemical basis for this derailment has remained a mystery. Here we report the discovery of a novel oxidoreductase (AclR) that represents the first-in-class enzyme catalyzing both a carbon-sulfur bond migration and spiro-ring formation, and that the acl pathway involves a cryptic acetylation as a prerequisite for the rearrangement. Genetic screening in A. oryzae identified aclR as the candidate for the complex biotransformation, and the aclR-deficient mutant provided the biosynthetic intermediate, unexpectedly harboring an acetyl group. In vitro assays showed that AclR alone promotes 1,2-sulfamyl migration, elimination of the acetoxy group, and spiroaminal formation. AclR features a thioredoxin oxidoreductase fold with a noncanonical CXXH motif that is distinct from the CXXC in the disulfide forming oxidase for the ETP biosynthesis. Crystallographic and mutational analyses of AclR revealed that the CXXH motif is crucial for catalysis, whereas the flavin-adenine dinucleotide is required as a support of the protein fold, and not as a redox cofactor. AclR proved to be a suitable bioinformatics handle to discover a number of related fungal gene clusters that potentially code for the biosynthesis of derailed ETP compounds. Our results highlight a specialized role of the thioredoxin oxidoreductase family enzyme in the ETP pathway and expand the chemical diversity of small molecules bearing an aberrant disulfide pharmacophore.

    DOI: 10.1021/jacs.0c08879

    PubMed

  3. 1,2,3-Triazine formation mechanism of the fairy chemical 2-azahypoxanthine in the fairy ring-forming fungus Lepista sordida. International journal

    Akinobu Ito, Jae-Hoon Choi, Waki Yokoyama-Maruyama, Mihaya Kotajima, Jing Wu, Tomohiro Suzuki, Yurika Terashima, Hyogo Suzuki, Hirofumi Hirai, David C Nelson, Yuta Tsunematsu, Kenji Watanabe, Tomohiro Asakawa, Hitoshi Ouchi, Makoto Inai, Hideo Dohra, Hirokazu Kawagishi

    Organic & biomolecular chemistry   Vol. 20 ( 13 ) page: 2636 - 2642   2022.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    2-Azahypoxanthine (AHX) was first isolated from the culture broth of the fungus Lepista sordida as a fairy ring-inducing compound. It has since been found that a large number of plants and mushrooms produce AHX endogenously and that AHX has beneficial effects on plant growth. The AHX molecule has an unusual, nitrogen-rich 1,2,3-triazine moiety of unknown biosynthetic origin. Here, we establish the biosynthetic pathway for AHX formation in L. sordida. Our results reveal that the key nitrogen sources that are responsible for the 1,2,3-triazine formation are reactive nitrogen species (RNS), which are derived from nitric oxide (NO) produced by NO synthase (NOS). Furthermore, RNS are also involved in the biochemical conversion of 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5'-monophosphate (AICAR) to AHX-ribotide (AHXR), suggesting that a novel biosynthetic route that produces AHX exists in the fungus. These findings demonstrate a physiological role for NOS in AHX biosynthesis as well as in biosynthesis of other natural products containing a nitrogen-nitrogen bond.

    DOI: 10.1039/d2ob00328g

    PubMed

  4. Induction of DNA Damage in Mouse Colorectum by Administration of Colibactin-producing <i>Escherichia coli</i>, Isolated from a Patient With Colorectal Cancer.

    Narita T, Tsunematsu Y, Miyoshi N, Komiya M, Hamoya T, Fujii G, Yoshikawa Y, Sato M, Kawanishi M, Sugimura H, Iwashita Y, Totsuka Y, Terasaki M, Watanabe K, Wakabayashi K, Mutoh M

    In vivo (Athens, Greece)   Vol. 36 ( 2 ) page: 628 - 634   2022.3

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    Language:English  

    DOI: 10.21873/invivo.12746

    PubMed

  5. Toward Engineered Biosynthesis of Drugs in Human Cells. International journal

    Shinya Matsuda, Yuta Tsunematsu, Takuma Matsushita, Yuji Ogata, Shihomi Hachiya, Shinji Kishimoto, Noriyuki Miyoshi, Kenji Watanabe

    Chembiochem : a European journal of chemical biology   Vol. 23 ( 4 ) page: e202100645   2022.2

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    Biosynthetic genes are not only responsible for the formation of bioactive substances but also suited for other applications including gene therapy. To test the feasibility of human cells producing antibiotics in situ when provided with a heterologous biosynthetic gene, we focused on cytochrome P450, the class of enzymes important in conferring bioactivity to natural product precursors. We selected Fma-P450 that plays a central role in the fumagillin antimicrobial biosynthesis in Aspergillus fumigatus to examine fungal metabolite production by HeLa cells that express fma-P450 heterologously. Here we show that HeLa cells harboring fma-P450 can biosynthesize 5-hydroxyl-β-trans-bergamoten and cytotoxic 5-epi-demethoxyfumagillol when supplemented with the nontoxic precursor β-trans-bergamotene. While the production level was insufficient to effect cell death, we demonstrate that programming human cells to autogenerate antibiotics by introducing a heterologous biosynthetic gene is feasible.

    DOI: 10.1002/cbic.202100645

    PubMed

  6. Association of Escherichia coli containing polyketide synthase in the gut microbiota with colorectal neoplasia in Japan. International journal

    Motoki Iwasaki, Rieko Kanehara, Taiki Yamaji, Ryoko Katagiri, Michihiro Mutoh, Yuta Tsunematsu, Michio Sato, Kenji Watanabe, Koji Hosomi, Yasuo Kakugawa, Hiroaki Ikematsu, Kinichi Hotta, Jun Kunisawa, Keiji Wakabayashi, Takahisa Matsuda

    Cancer science   Vol. 113 ( 1 ) page: 277 - 286   2021.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    Escherichia coli containing polyketide synthase in the gut microbiota (pks+ E coli) produce a polyketide-peptide genotoxin, colibactin, and are suspected to play a role in the development of colorectal neoplasia. To clarify the role of pks+ E coli in the early stage of tumorigenesis, we investigated whether the pks status of E coli was associated with the prevalence of colorectal neoplasia. This cross-sectional analysis of data from a prospective cohort in Izu Oshima, Japan included asymptomatic residents aged 40-79 years who underwent screening colonoscopy and provided a stool sample. We identified 543 participants with colorectal neoplasia (22 colorectal cancer and 521 adenoma) as cases and 425 participants with normal colon as controls. The pks status of E coli was assayed using stool DNA and specific primers that detected pks+ E coli. The proportion of pks+ E coli was 32.6% among cases and 30.8% among controls. Compared with those with pks- E coli, the odds ratio (OR) (95% confidence interval) for participants with pks+ E coli was 1.04 (0.77-1.41) after adjusting for potential confounders. No statistically significant associations were observed regardless of tumor site or number of colorectal adenoma lesions. However, stratified analyses revealed increased ORs among participants who consumed cereals over the median intake or vegetables under the median intake. Overall, we found no statistically significant association between pks+ E coli and the prevalence of colorectal adenoma lesions among this Japanese cohort. However, positive associations were suggested under certain intake levels of cereals or vegetables.

    DOI: 10.1111/cas.15196

    PubMed

  7. Mother-to-infant transmission of the carcinogenic colibactin-producing bacteria. International journal

    Yuta Tsunematsu, Koji Hosomi, Jun Kunisawa, Michio Sato, Noriko Shibuya, Emiko Saito, Haruka Murakami, Yuko Yoshikawa, Yuji Iwashita, Noriyuki Miyoshi, Michihiro Mutoh, Hideki Ishikawa, Haruhiko Sugimura, Motohiko Miyachi, Keiji Wakabayashi, Kenji Watanabe

    BMC microbiology   Vol. 21 ( 1 ) page: 235 - 235   2021.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND: The Escherichia coli strain that is known to produce the genotoxic secondary metabolite colibactin is linked to colorectal oncogenesis. Therefore, understanding the properties of such colibactin-positive E. coli and the molecular mechanism of oncogenesis by colibactin may provide us with opportunities for early diagnosis or prevention of colorectal oncogenesis. While there have been major advances in the characterization of colibactin-positive E. coli and the toxin it produces, the infection route of the clb + strain remains poorly characterized. RESULTS: We examined infants and their treatments during and post-birth periods to examine potential transmission of colibactin-positive E. coli to infants. Here, analysis of fecal samples of infants over the first month of birth for the presence of a colibactin biosynthetic gene revealed that the bacterium may be transmitted from mother to infant through intimate contacts, such as natural childbirth and breastfeeding, but not through food intake. CONCLUSIONS: Our finding suggests that transmission of colibactin-positive E. coli appears to be occurring at the very early stage of life of the newborn and hints at the possibility of developing early preventive measures against colorectal cancer.

    DOI: 10.1186/s12866-021-02292-1

    PubMed

  8. Stool pattern is associated with not only the prevalence of tumorigenic bacteria isolated from fecal matter but also plasma and fecal fatty acids in healthy Japanese adults. International journal

    Daiki Watanabe, Haruka Murakami, Harumi Ohno, Kumpei Tanisawa, Kana Konishi, Kikue Todoroki-Mori, Yuta Tsunematsu, Michio Sato, Yuji Ogata, Noriyuki Miyoshi, Naoto Kubota, Jun Kunisawa, Keiji Wakabayashi, Tetsuya Kubota, Kenji Watanabe, Motohiko Miyachi

    BMC microbiology   Vol. 21 ( 1 ) page: 196 - 196   2021.6

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    BACKGROUND: Colibactin-producing Escherichia coli containing polyketide synthase (pks+ E. coli) has been shown to be involved in colorectal cancer (CRC) development through gut microbiota analysis in animal models. Stool status has been associated with potentially adverse gut microbiome profiles from fecal analysis in adults. We examined the association between stool patterns and the prevalence of pks+ E. coli isolated from microbiota in fecal samples of 224 healthy Japanese individuals. RESULTS: Stool patterns were determined through factorial analysis using a previously validated questionnaire that included stool frequency, volume, color, shape, and odor. Factor scores were classified into tertiles. The prevalence of pks+ E. coli was determined by using specific primers for pks+ E. coli in fecal samples. Plasma and fecal fatty acids were measured via gas chromatography-mass spectrometry. The prevalence of pks+ E. coli was 26.8%. Three stool patterns identified by factorial analysis accounted for 70.1% of all patterns seen (factor 1: lower frequency, darker color, and harder shape; factor 2: higher volume and softer shape; and factor 3: darker color and stronger odor). Multivariable-adjusted odds ratios (95% confidence intervals) of the prevalence of pks+ E. coli for the highest versus the lowest third of the factor 1 score was 3.16 (1.38 to 7.24; P for trend = 0.006). This stool pattern exhibited a significant positive correlation with fecal isobutyrate, isovalerate, valerate, and hexanoate but showed a significant negative correlation with plasma eicosenoic acid and α-linoleic acid, as well as fecal propionate and succinate. No other stool patterns were significant. CONCLUSIONS: These results suggest that stool patterns may be useful in the evaluation of the presence of tumorigenic bacteria and fecal fatty acids through self-monitoring of stool status without the requirement for specialist technology or skill. Furthermore, it may provide valuable insight about effective strategies for the early discovery of CRC.

    DOI: 10.1186/s12866-021-02255-6

    PubMed

  9. Isolation of New Colibactin Metabolites from Wild-Type Escherichia coli and In Situ Trapping of a Mature Colibactin Derivative. Reviewed International journal

    Tao Zhou, Yuichiro Hirayama, Yuta Tsunematsu, Nanami Suzuki, Seiji Tanaka, Nahoko Uchiyama, Yukihiro Goda, Yuko Yoshikawa, Yuji Iwashita, Michio Sato, Noriyuki Miyoshi, Michihiro Mutoh, Hideki Ishikawa, Haruhiko Sugimura, Keiji Wakabayashi, Kenji Watanabe

    Journal of the American Chemical Society   Vol. 143 ( 14 ) page: 5526 - 5533   2021.3

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    Colibactin is a polyketide-nonribosomal peptide hybrid secondary metabolite that can form interstrand cross-links in double-stranded DNA. Colibactin-producing Escherichia coli has also been linked to colorectal oncogenesis. Thus, there is a strong interest in understanding the role colibactin may play in oncogenesis. Here, using the high-colibactin-producing wild-type E. coli strain we isolated from a clinical sample with the activity-based fluorescent probe we developed earlier, we were able to identify colibactin 770, which was recently identified and proposed as the complete form of colibactin, along with colibactin 788, 406, 416, 420, and 430 derived from colibactin 770 through structural rearrangements and solvolysis. Furthermore, we were able to trap the degrading mature colibactin species by converting the diketone moiety into quinoxaline in situ in the crude culture extract to form colibactin 860 at milligram scale. This allowed us to determine the stereochemically complex structure of the rearranged form of an intact colibactin, colibactin 788, in detail. Furthermore, our study suggested that we were capturing only a few percent of the actual colibactin produced by the microbe, providing a crude quantitative insight into the inherent instability of this compound. Through the structural assignment of colibactins and their degradative products by the combination of LC-HRMS and NMR spectroscopies, we were able to elucidate further the fate of inherently unstable colibactin, which could help acquire a more complete picture of colibactin metabolism and identify key DNA adducts and biomarkers for diagnosing colorectal cancer.

    DOI: 10.1021/jacs.1c01495

    PubMed

  10. Catalytic mechanism and endo-to-exo selectivity reversion of an octalin-forming natural Diels-Alderase

    Michio Sato, Shinji Kishimoto, Mamoru Yokoyama, Cooper S. Jamieson, Kazuto Narita, Naoya Maeda, Kodai Hara, Hiroshi Hashimoto, Yuta Tsunematsu, Kendall N. Houk, Yi Tang, Kenji Watanabe

    NATURE CATALYSIS   Vol. 4 ( 3 ) page: 223 - +   2021.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE RESEARCH  

    We have previously reported the identification of CghA, a proposed Diels-Alderase responsible for the formation of the bicyclic octalin core of the fungal secondary metabolite Sch210972. Here we show the crystal structure of the CghA-product complex at a resolution of 2.0 angstrom. Our result provides the second structural determination of eukaryotic Diels-Alderases and adds yet another fold to the family of proteins reported to catalyse [4 + 2] cycloaddition reactions. Site-directed mutagenesis-coupled kinetic characterization and computational analyses allowed us to identify key catalytic residues and propose a possible catalytic mechanism. Most interestingly, we were able to rationally engineer CghA such that the mutant was able to catalyse preferentially the formation of the energetically disfavoured exo adduct. This work expands our knowledge and understanding of the emerging and potentially widespread class of natural enzymes capable of catalysing stereoselective Diels-Alder reactions and paves the way towards developing enzymes potentially useful in various bio/synthetic applications.

    DOI: 10.1038/s41929-021-00577-2

    Web of Science

  11. o-Anisidine Dimer, 2-Methoxy-N4-(2-methoxyphenyl) Benzene-1,4-diamine, in Rat Urine Associated with Urinary bladder Carcinogenesis. Reviewed International journal

    Takuma Kobayashi, Takeshi Toyoda, Yuya Tajima, Shinji Kishimoto, Yuta Tsunematsu, Michio Sato, Kohei Matsushita, Takanori Yamada, Yuko Shimamura, Shuichi Masuda, Masako Ochiai, Kumiko Ogawa, Kenji Watanabe, Takeji Takamura-Enya, Yukari Totsuka, Keiji Wakabayashi, Noriyuki Miyoshi

    Chemical research in toxicology   Vol. 34 ( 3 ) page: 912 - 919   2021.2

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    Monocyclic aromatic amines, o-toluidine (o-Tol) and its structural analog o-anisidine (o-Ans), are IARC Group 1 and Group 2A urinary bladder carcinogens, respectively, and are involved in metabolic activation and DNA damage. Our recent study revealed that 2-methyl-N4-(2-methylphenyl) benzene-1,4-diamine (MMBD), a p-semidine-type homodimer of o-Tol, was detected and identified in an in vitro reaction of o-Tol with S9 mix and in vivo urinary samples of o-Tol-exposed rats. Potent mutagenic, genotoxic, and cytotoxic activities were reported with MMBD, suggesting its involvement in urinary bladder carcinogenesis. However, it remains unknown whether o-Ans is converted to active metabolites to induce DNA damage in a similar manner as o-Tol. In this study, we report that a novel o-Ans metabolite, 2-methoxy-N4-(2-methoxyphenyl) benzene-1,4-diamine (MxMxBD), a dimer by head-to-tail binding (p-semidine form), was for the first time identified in o-Ans-exposed rat urine. MxMxBD induced a stronger mutagenicity in N-acetyltransferase overexpressed Salmonella typhimurium strains and potent genotoxicity and cytotoxicity in human bladder carcinoma T24 cells compared with o-Ans. These results suggest that MxMxBD may to some extent contribute toward urinary bladder carcinogenesis. In addition to homodimerization, such as MxMxBD, heterodimerizations were observed when o-Ans was coincubated with o-Tol or aniline (Ani) in in vitro reactions with S9 mix. This study highlights the important consideration of homodimerizations and heterodimerizations of monocyclic aromatic amines, including o-Ans, o-Tol, and Ani, in the evaluation of the combined exposure risk of bladder carcinogenesis.

    DOI: 10.1021/acs.chemrestox.0c00536

    PubMed

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Books 3

  1. 担子菌休眠型生合成遺伝子の覚醒による 新規天然物の発見とその菌糸成長・子実体形成作用

    恒松雄太( Role: Sole author)

    アグリバイオ11月号 北隆館  2021.10 

  2. 生合成遺伝子クラスターの高度強制発現による合成生物学が拓く有用天然物の創製

    中沢威人, 恒松雄太, 石川格靖, 渡辺賢二

    生物工学会誌 90, 289-292, 2012. 

  3. 出芽酵母発現システムを利用した天然物の生物合成

    恒松雄太, 守屋央朗, 渡辺賢二

    化学と生物 50, 163-174, 2012. 

Presentations 56

  1. 恒松雄太, 渡辺賢二:Harnessing the Biosynthetic Pathway in Filamentous Fungus, Osaka University-Universiti Brunei Darussalam Joint symposium, 2018年2月21日 (Brunei Darussalam)2018年2月21日(ポスター)

  2. (招待講演)恒松雄太:糸状菌ゲノム情報を活用した新規天然物の探索とその生合成, 静岡大学超領域研究推進本部 第12回超領域研究会, 2018年12月3日 (静岡県静岡市)2018年12月3日(口頭)

  3. (受賞講演)恒松雄太:糸状菌ゲノム情報を活用した天然物探索とその生合成機構解明, 日本生薬学会第66回年会(東京), 2019年9月22-23日 (東京都港区)(口頭)

  4. (招待講演)恒松雄太, 渡辺賢二:Dissecting and harnessing fungal biosynthetic pathway, International Congress on Pure & Applied Chemistry Langkawi (ICPAC Langkawi) 2018, 2018年10月30日-11月2日 (Langkawi, Malaysia)2018年10月30日-11月2日(口頭)

  5. (招待講演)恒松雄太:生合成遺伝子制御に基づく天然物創薬リバイバル, 日本薬学会第139年会(千葉), 2019年3月20-23日 (千葉県千葉市)(口頭)

  6. (招待講演)恒松雄太:生合成遺伝子制御に基づく天然物創薬リバイバル, 第 7 回慶應有機化学若手シンホ_シ_ウム, 2019年5月11日 (神奈川県横浜市)(口頭)

  7. (招待講演)恒松雄太:糸状菌ゲノムが拡張する天然有機化合物研究, 糸状菌分子生物学研究会若手の会第7回ワークショップ, 2019年11月5-6日 (北海道空知郡)(口頭)

  8. 高西潤, 桝谷貴洋, 中沢威人, 恒松雄太, 佐藤道大, 渡辺賢二:レギュレータ-遺伝子改変法によるCoprinopsis属担子菌の新規天然物生合成経路活性化, 日本農芸化学会2019年度東京大会, 2019年3月24-27日 (東京都世田谷区)(口頭)

  9. 久冨優太, 小田美光, 下原千晶, 恒松雄太, 佐藤道大, 平山裕一郎, 三好規之, 岩下雄二, 吉川悠子, 八木孝司, 椙村春彦, 若林敬二, 渡辺賢二, 川西優喜:コリバクチン産生大腸菌のin vitro遺伝毒性試験, 日本環境変異原学会第47回大会, 2018年11月2-3日 (京都府京都市)2018年11月2-3日(ポスター)

  10. 佐藤道大, 平山裕一郎, 恒松雄太, 東口ふみ, 吉川悠子, 三好規之, 岩下雄二, 椙村春彦, 若林敬二, 武藤倫弘, 石川秀樹, 渡辺賢二:遺伝毒性物質コリハ_クチン生産菌の高感度多検体検出法の確立, がん予防学術大会2019札幌, 2019年6月28-29日 (北海道札幌市)(口頭)

  11. 佐藤道大, 籠浦倫美, 恒松雄太, 竹仲由希子, 棚橋孝雄, 渡辺賢二:共生型子嚢菌Pyrenula sp.由来pyrenulic acid類の生合成研究, 日本薬学会第139年会(千葉), 2019年3月20-23日 (千葉県千葉市)(口頭)

  12. 佐藤道大, 籠浦倫美, 恒松雄太, 竹仲由希子, 棚橋孝雄, 渡辺賢二:共生型子嚢菌Pyrenula sp.由来pyrenulic acid類の生合成研究, 日本農芸化学会2019年度東京大会, 2019年3月24-27日 (東京都世田谷区)(口頭)

  13. 前田直哉, 恒松雄太, Hertweck Christian, 渡辺賢二:Aspirochlorine 生合成経路におけるスピロ環形成を担う新規酵素の発見と機能解析, 日本薬学会第139年会(千葉), 2019年3月20-23日 (千葉県千葉市)(口頭)

  14. 前田直哉, 恒松雄太, Hertweck Christian, 渡辺賢二:Aspirochlorine 生合成経路におけるスピロ環形成を担う新規酵素の発見と機能解析, 日本農芸化学会2019年度東京大会, 2019年3月24-27日 (東京都世田谷区)(口頭)

  15. 前田直哉, 恒松雄太, 原幸大, 橋本博, Christian Hertweck, 渡辺賢二:Aspirochlorine 生合成経路におけるスピロ環形成酵素の発見と機能解析, 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2018, 2018年11月14日 (静岡県静岡市)2018年11月14日(口頭)

  16. 前田直哉, 恒松雄太, 渡辺賢二:Revealing an unprecedented biosynthetic pathway in Aspergillus oryzae, 30th International Symposium on the Chemistry of Natural Products and the 10th International Congress on Biodiversity (ISCNP30 & ICOB10), 2018年11月25-29日 (Athens, Greec

  17. 岸本真治, 原幸大, 石川格靖, 山田陽香, 恒松雄太, 橋本博, Kendall N. Houk, Yi Tang, 渡辺賢二:キノロン骨格を形成する糸状菌酵素シクロペナーゼの反応機構解析, 第60回天然有機化合物討論会, 2018年9月26-28日 (福岡県久留米市)2018年9月26-28日(口頭)

  18. 岸本真治, 松下拓磨, 原幸大, 橋本博, 恒松雄太, 渡辺賢二:ポリケチド鎖をメチル化するPsoF-MTの構造解析とメチル化部位選択性の解明, 日本生薬学会第65回年会(広島), 2018年9月16-17日 (広島県広島市)2018年9月16-17日(口頭)

  19. 岸本真治, 横山葵, 佐藤道大, 前田直哉, 恒松雄太, 原幸大, 橋本博, 渡辺賢二:立体選択的なDiels-Alder反応を触媒する酵素CghAの触媒機構解明, 日本薬学会第139年会(千葉), 2019年3月20-23日 (千葉県千葉市)(口頭)

  20. 岸本真治, 横山葵, 佐藤道大, 前田直哉, 恒松雄太, 原幸大, 橋本博, 渡辺賢二:糸状菌由来酵素 CghA による立体選択的な Diels-Alder 反応の機構解明, 日本農芸化学会2019年度東京大会, 2019年3月24-27日 (東京都世田谷区)(ポスター)

  21. 岸本真治, 横山葵, 佐藤道大, 前田直哉, 恒松雄太, 原幸大, 橋本博, 渡辺賢二:糸状菌由来酵素 CghA による立体選択的な Diels-Alder 反応の機構解明, 日本農芸化学会2019年度東京大会, 2019年3月24-27日 (東京都世田谷区)(口頭)

  22. 岸本真治, 横山葵, 成田一仁, 佐藤道大, 前田直哉, 恒松雄太, 原幸大, 橋本博, 渡辺賢二:糸状菌由来Diels_Alderase CghAの反応機構解明, 日本生薬学会第66回年会(東京), 2019年9月22-23日 (東京都港区)(口頭)

  23. 平山裕一郎, 松崎信生, 玉舟亮太, 恒松雄太, 佐藤道大, 吉川悠子, 三好規之, 岩下雄二, 椙村春彦, 若林敬二, 武藤倫弘, 石川秀樹, 渡辺賢二:大腸がんリスク因子物質コリバクチンの生産菌検出に関する化学的研究, がん予防学術大会2018高松, 2018年6月27-28日 (香川県高松市)2018年6月27-28日(ポスター)

  24. 平山裕一郎, 玉舟亮太, 恒松雄太, 渡辺賢二:大腸がんリスク因子物質コリバクチン関連化合物の特定, 日本薬学会第139年会(千葉), 2019年3月20-23日 (千葉県千葉市)(口頭)

  25. 平山裕一郎, 玉舟亮太, 恒松雄太, 渡辺賢二:大腸がんリスク因子物質コリバクチン関連化合物の特定, 日本農芸化学会2019年度東京大会, 2019年3月24-27日 (東京都世田谷区)(口頭)

  26. 平山裕一郎, 玉舟亮太, 松崎信生, 恒松雄太, 渡辺賢二:大腸がんリスク因子物質コリバクチンの生産菌検出に関する化学的研究, 第22回天然薬物の開発と応用シンポジウム, 2018年10月7-8日 (熊本県熊本市)2018年10月7-8日(ポスター)

  27. 平山裕一郎, 田島悠也, 恒松雄太, 三好規之, 若林敬二, 渡辺 賢二:検出困難な遺伝毒性物質コリバクチンを見出すためのDNA付加体の解析, 日本生薬学会第65回年会(広島), 2018年9月16-17日 (広島県広島市)2018年9月16-17日(口頭)

  28. 平山裕一郎, 鈴木七海, 佐藤道大, 恒松雄太, 内山奈穂子, 合田幸広, 渡辺賢二:大腸菌が生合成する大腸がんリスク因子化合物コリバクチンの検出と構造解明, 第61回天然有機化合物討論会, 2019年9月11-13日 (広島県広島市)(ポスター)

  29. 恒松雄太, 吉川悠子, 三好規之, 武藤倫弘, 山地太樹, 張萌琳, 岩崎基, 松田尚久, 細見晃司, 國澤純, 若林敬二, 石川秀樹, 渡辺賢二:大腸発がんリスク因子コリバクチンの生産性を制御する要因の解明, がん予防学術大会2019札幌, 2019年6月28-29日 (北海道札幌市)(ポスター)

  30. 恒松雄太, 渡辺賢二:Dissecting and harnessing fungal biosynthetic pathway, 30th International Symposium on the Chemistry of Natural Products and the 10th International Congress on Biodiversity (ISCNP30 & ICOB10), 2018年11月25-29日 (Athens, Greece)2018年11月25-29日(口頭)

  31. 恒松雄太:ヒト細菌叢を構成する微生物由来の天然物化学, 第54回天然物談話会, 2019年7月3-5日 (北海道札幌市)(ポスター)

  32. 成田一仁, 岸本真治, 横山葵, 恒松雄太, 佐藤道大, 渡辺賢二:Diels-Alder反応を触媒する酵素CghAの基質合成および反応解析, 日本薬学会第139年会(千葉), 2019年3月20-23日 (千葉県千葉市)(口頭)

  33. 成田一仁, 岸本真治, 横山葵, 恒松雄太, 佐藤道大, 渡辺賢二:Diels-Alder反応を触媒する酵素CghAの基質合成および反応解析, 日本農芸化学会2019年度東京大会, 2019年3月24-27日 (東京都世田谷区)(口頭)

  34. 成田一仁, 岸本真治, 横山葵, 恒松雄太, 佐藤道大, 渡辺賢二:立体選択的反応機構の解明を目指したDiels-Alder反応を触媒するCghAの基質合成研究, 日本生薬学会第65回年会(広島), 2018年9月16-17日 (広島県広島市)2018年9月16-17日(口頭)

  35. 成田一仁, 岸本真治, 横山葵, 恒松雄太, 佐藤道大, 渡辺賢二:立体選択的反応機構の解明を目指したDiels-Alder反応を触媒するCghAの基質合成研究, 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2018, 2018年11月14日 (静岡県静岡市)2018年11月14日(口頭)

  36. 松下拓磨, 原幸大, 恒松雄太, 橋本博, 渡辺賢二:フラビン依存型モノオキシゲナーゼによるスピロ環化形成機構の解明, 日本生薬学会第66回年会(東京), 2019年9月22-23日 (東京都港区)(口頭)

  37. 松下拓磨, 岸本真治, 山本剛, 原幸大, 恒松雄太, 橋本博, 渡辺賢二:PsoFのメチルトランスフェラーセ_ト_メインの構造解析, 2017年度量子ビームサイエンスフェスタ, 2018年3月2-4日 (茨城県つくば市)2018年3月2-4日(ポスター)

  38. 松下拓磨, 岸本真治, 山本剛, 原幸大, 恒松雄太, 橋本博, 渡辺賢二:PsoFメチルトランスフェラーゼドメインの基質認識機構の解明と酵素機能の改変, 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2018, 2018年11月14日 (静岡県静岡市)2018年11月14日(口頭)

  39. 桝谷貴洋, 平山裕一郎, 高西潤, 恒松雄太, 佐藤道大, 大高潤之介, 本山高幸, 長田裕之, 渡辺賢二:複雑な酸化経路を含むキノコ由来lagopodin類の生合成研究, 日本薬学会第139年会(千葉), 2019年3月20-23日 (千葉県千葉市)(口頭)

  40. 桝谷貴洋, 平山裕一郎, 高西潤, 恒松雄太, 佐藤道大, 大高潤之介, 本山高幸, 長田裕之, 渡辺賢二:複雑な酸化経路を含むキノコ由来lagopodin類の生合成研究, 日本農芸化学会2019年度東京大会, 2019年3月24-27日 (東京都世田谷区)(口頭)

  41. 桝谷貴洋, 平山裕一郎, 高西潤, 田村優依, 中沢威人, 恒松雄太, 佐藤道大, 渡辺賢二:キノコ由来天然物ラゴポジン類の生合成研究, 日本生薬学会第65回年会(広島), 2018年9月16-17日 (広島県広島市)2018年9月16-17日(口頭)

  42. 横山葵, 佐藤 道大, 前田 直哉, 恒松 雄太, 原 幸大, 橋本 博, 渡辺 賢二:酵素_生成物複合体を用いたCghAの立体選択的反応機構の解明, 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2018, 2018年11月14日 (静岡県静岡市)2018年11月14日(口頭)

  43. 横山葵, 佐藤道大, 前田直哉, 恒松雄太, 原幸大, 橋本博, 渡辺賢二:酵素_生成物複合体を用いたCghAの立体選択的反応機構の解明, 日本生薬学会第65回年会(広島), 2018年9月16-17日 (広島県広島市)2018年9月16-17日(口頭)

  44. 横山葵, 前田直哉, 岸本真治, 佐藤道大, 原幸大, 恒松雄太, 橋本博, 渡辺賢二:新規構造を持つ環化酵素CghAのX線結晶構造解析とその立体選択的反応機構 , 2017年度量子ビームサイエンスフェスタ, 2018年3月2-4日 (茨城県つくば市)2018年3月2-4日(ポスター)

  45. 浅井しほり, 恒松雄太, 高西潤, 桝谷貴洋, 佐藤道大, 大高潤之介, 本山高幸, 長田裕之, 渡辺賢二:キノコ由来天然物lagopodin類の生合成研究, 日本生薬学会第66回年会(東京), 2019年9月22-23日 (東京都港区)(口頭)

  46. 玉舟亮太, 平山裕一郎, 恒松雄太, 佐藤道大, 渡辺賢二:大腸がんリスク因子物質コリバクチンの生産菌を検出する分子プローブ, 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2018, 2018年11月14日 (静岡県静岡市)2018年11月14日(口頭)

  47. 玉舟亮太, 平山裕一郎, 恒松雄太, 渡辺賢二:大腸がんリスク因子物質コリバクチンの生産菌を検出する分子プローブ, 第22回天然薬物の開発と応用シンポジウム, 2018年10月7-8日 (熊本県熊本市)2018年10月7-8日(口頭)

  48. 田村優依, 桝谷貴洋, 恒松雄太, 佐藤道大, 渡辺賢二:レギュレーターを利用した冬虫夏草代謝産物の生産変化, 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2018, 2018年11月14日 (静岡県静岡市)2018年11月14日(口頭)

  49. 籠浦倫美, 佐藤道大, 恒松雄太, 竹仲由希子, 棚橋孝雄, 渡辺賢二:共生型子嚢菌Pyrenula sp.に由来するPyrenulic acid類の生合成遺伝子の同定, 日本生薬学会第65回年会(広島), 2018年9月16-17日 (広島県広島市)2018年9月16-17日(口頭)

  50. 籠浦倫美, 佐藤道大, 恒松雄太, 竹仲由希子, 棚橋孝雄, 渡辺賢二:共生型子嚢菌Pyrenula sp.に由来するPyrenulic acid類の生合成遺伝子の同定, 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2018, 2018年11月14日 (静岡県静岡市)2018年11月14日(口頭)

  51. 鈴木七海, 恒松雄太, 平山裕一郎, 尾形勇二, 佐藤道大, 辻本恭, 内山奈穂子, 袴塚高志, 合田幸広, 渡辺賢二:大腸がんリスク因子物質コリバクチン関連化合物の構造決定, 日本生薬学会第66回年会(東京), 2019年9月22-23日 (東京都港区)(口頭)

  52. 高西潤, 桝谷貴洋, 中沢威人, 恒松雄太, 佐藤道大, 渡辺賢二:Coprinopsis属担子菌のレギュレータ−遺伝子操作による化合物生産, 日本病院薬剤師会東海ブロック・日本薬学会東海支部合同学術大会2018, 2018年11月14日 (静岡県静岡市)2018年11月14日(口頭)

  53. 高西潤, 桝谷貴洋, 中沢威人, 恒松雄太, 佐藤道大, 渡辺賢二:キノコ由来二次代謝産物の過剰生産系の構築, 日本生薬学会第65回年会(広島), 2018年9月16-17日 (広島県広島市)2018年9月16-17日(口頭)

  54. 高西潤, 桝谷貴洋, 中沢威人, 恒松雄太, 佐藤道大, 渡辺賢二:レギュレータ-遺伝子改変法によるCoprinopsis属担子菌の新規天然物生合成経路活性化, 日本薬学会第139年会(千葉), 2019年3月20-23日 (千葉県千葉市)(口頭)

  55. (招待講演)渡辺賢二, 恒松雄太, 平山裕一郎, 東口ふみ, 佐藤道大, 吉川悠子, 三好規之, 岩下雄二, 椙村春彦, 武藤倫弘, 石川秀樹, 若林敬二:大腸か_んリスク因子コリハ_クチン産生菌の増殖抑制を目的としたハ_イロッ ト介入試験, がん予防学術大会2019札幌, 2019年6月28-29日 (北海道札幌市)(口頭)

  56. "前田直哉, 恒松雄太, 原幸大, 橋本博, 渡辺賢二:Aspirochlorine生合成経路におけるスヒ_ロ環形成を担う新規酵素の発見と機能 解析, 第61回天然有機化合物討論会, 2019年9月11-13日 (広島県広島市)(ポスター)"

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KAKENHI (Grants-in-Aid for Scientific Research) 4

  1. 超炭素鎖有機分子の生合成

    2022.4 - 2028.3

    科学技術振興機構(JST)  創発的研究支援事業 

      More details

    Authorship:Principal investigator 

  2. 複雑性創出型化学/生物ハイブリッド合成に基づく赤痢アメーバ治療薬創製

    2020.12 - 2023.3

    AMED  新興・再興感染症研究基盤創生事業 多分野融合研究領域 

      More details

    Authorship:Principal investigator 

  3. The molecular and functional generality of the Basidiomycetous siderophore that promotes mycelial growth and fruiting body formation

    Grant number:20K05866  2020.4 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

  4. Integrating Computational and Experimental Approaches for New Paradigms in Molecule Synthesis

    Grant number:19KK0150  2019.10 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))