Updated on 2025/03/19

写真a

 
IDA Hiroki
 
Organization
Graduate School of Engineering Electronics 2 Lecturer
Graduate School
Graduate School of Engineering
Undergraduate School
School of Engineering Electrical Engineering, Electronics, and Information Engineering
Title
Lecturer
External link

Degree 3

  1. 博士（学術） （ 2019.3   東北大学 ） 

  2. 修士（学術） （ 2016.3   東北大学 ） 

  3. 学士（工学） （ 2014.3   東北大学 ） 

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Research Interests 1

  1. scanning ion conductance microscopy

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Research Areas 1

  1. Nanotechnology/Materials / Nanobioscience

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Research History 2

  1. Nagoya University   Graduate School of Engineering   Lecturer

    2023.3

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  2. Nagoya University   Designated associate professor

    2022.4 - 2023.2

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Papers 6

  1. Direct Extraction and Evaluation of Intraluminal Vesicles Inside a Single Cell. Reviewed International journal

    Hiroki Ida, Takeshi Yoshida, Akichika Kumatani, Rikinari Hanayama, Yasufumi Takahashi

    Nano letters     2025.2

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    Because endogenous extracellular vesicles are involved in important physiological functions, various techniques have been developed for their isolation and evaluation. However, methods for evaluating endogenous vesicles within cells are limited. This study presents a technique for the direct extraction and evaluation of intraluminal vesicles (ILVs). This technique combines scanning ion conductance microscopy, electrochemical syringes, and confocal microscopy to extract specific structures within a living cell, achieving high spatial resolution and accuracy at the femtoliter scale. This approach allowed the direct collection of CD63(+) vesicles from HEK293 CD63-pHluorin-RFP cells and showed that their RNA expression profiles were different from those recovered from cytosol and extracellular vesicles isolated by ultracentrifuge. It also identified a subset specifically containing hsa-miR-145-5p and allowed for direct assessment of the local accumulation of miRNAs in cells. This technique is expected to become a powerful tool for evaluating the contents of ILVs within living cells.

    DOI： 10.1021/acs.nanolett.4c06315

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  2. EMT-Induced Morphological Variations on Living Cell Membrane Surface Reviewed

    Hiroki Ida, Noriko Taira, Yuji Nashimoto, Akichika Kumatani, Yasufumi Takahashi, Hitoshi Shiku

    Analytical Chemistry   Vol. 97 ( 1 ) page： 312 - 318   2025.1

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    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Chemical Society (ACS)  

    DOI： 10.1021/acs.analchem.4c04204

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  3. Comprehensive electrochemical imaging analyses of redox activities correlated to multilayer graphene and graphite structures Reviewed

    Akichika Kumatani, Chiho Miura, Hiroki Ida, Yasufumi Takahashi, Yuichi Ikuhara, Hitoshi Shiku, Tomokazu Matsue, Takeru Okada

    Electrochimica Acta   Vol. 499   page： 144688 - 144688   2024.9

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.electacta.2024.144688

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  4. Emergence of electrochemical catalytic activity via an electrochemical-probe on defective transition metal dichalcogenide nanosheets Reviewed

    A. Kumatani, H. Ogawa, T. Endo, J. Lustikova, H. Ida, Y. Takahashi, Y. Miyata, Y. Ikuhara, H. Shiku, Y. Wakayama

    APL Energy   Vol. 2 ( 1 ) page： 016107   2024.2

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    Publishing type：Research paper (scientific journal)   Publisher：AIP Publishing  

    Two-dimensional transition metal dichalcogenides (2D TMDs) have shown exceptional electrochemical catalytic activity for the efficient generation of hydrogen through electrochemical water splitting. In the case of molybdenum disulfide (MoS2), a prominent member of 2D TMDs, the electrochemically active sites primarily reside at the edges, while the basal plane, which constitutes the majority of the MoS2 structure, remains relatively inactive. In this study, we aimed to activate the inert sites of the basal plane with some defective structure for hydrogen evolution reaction (HER) by employing an electrochemical-probe in combination with voltage sweeping. The initiation of HER at these previously inactive sites was visualized and confirmed using scanning electrochemical cell microscopy (SECCM). Our findings reveal that the enhanced HER activity originates from surface defects induced by the probing process.

    DOI： 10.1063/5.0175653

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  5. Surface morphology live-cell imaging reveals how macropinocytosis inhibitors affect membrane dynamics (vol 441, 141783, 2023) Open Access

    Ida, H; Taira, N; Azuma, K; Kumatani, A; Akishiba, M; Futaki, S; Takahashi, Y; Shiku, H

    ELECTROCHIMICA ACTA   Vol. 470   2023.12

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    Publisher：Electrochimica Acta  

    The authors regret that Supporting Information Movie S6 was incorrectly the same data as Supporting Information Movie S1. The correct Supporting Information Movie S6 is shown at doi:10.1016/j.electacta.2023.143278. The authors would like to apologise for any inconvenience caused.

    DOI： 10.1016/j.electacta.2023.143278

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  6. A noncanonical endocytic pathway is involved in the internalization of 3 μm polystyrene beads into HeLa cells. Reviewed International journal

    Hisaaki Hirose, Masashi Maekawa, Hiroki Ida, Masashi Kuriyama, Yasufumi Takahashi, Shiroh Futaki

    Biomaterials science   Vol. 10 ( 24 ) page： 7093 - 7102   2022.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    Extracellular fine particles of various sizes and origins can be taken up by cells, affecting their function. Understanding the cellular uptake processes is crucial for understanding the cellular effects of these particles and the development of means to control their internalization. Although macropinocytosis is a possible pathway for the cellular uptake of particles larger than 0.2 μm, its contribution to cellular uptake in non-phagocytic cells is controversial. Using 3 μm polystyrene beads as a model particle, we aimed to assess the detailed modes of their cellular uptake by non-phagocytic HeLa cells. Cellular uptake was assessed using confocal, scanning electron, and scanning ion conductance microscopy analyses, together with inhibitor studies. Our results revealed that 3 μm beads were taken up by HeLa cells by an actin-, cholesterol-, and membrane protrusions-dependent noncanonical endocytic pathway, different from the canonical macropinocytic and phagocytic pathways. Our work provides a framework for studying the cellular uptake of extracellular fine particles.

    DOI： 10.1039/d2bm01353c

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Presentations 1

  1. ナノピペットを用いた単一オルガネラスケールでの細胞内局所染色

    井田 大貴, 手島 慶大, 林 久美子, 高橋 康史

    第76回日本細胞生物学会大会  2024.7.19 

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    Event date： 2024.7

    Language：Japanese   Presentation type：Poster presentation  

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KAKENHI (Grants-in-Aid for Scientific Research) 2

  1. 細胞老化評価のための長期ナノ動態計測システムの創出

    Grant number：22K14703  2022.4 - 2024.3

    科学研究費助成事業  若手研究

    井田 大貴

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    Authorship：Principal investigator 

    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

    細胞は一般的な光学顕微鏡では観察できないナノメートルレベルでの変化が連続的に生じているが、ひとつの細胞のナノ動態を長期的に観察する事は難しかった。本研究では、非侵襲かつナノスケールで細胞表面形状を取得可能な走査型イオンコンダクタンス顕微鏡による計測を細胞培養環境下で実行可能な新規装置系を開発する。また、開発した装置を用いて数日スパンで形態や代謝活性等が連続的に変化する細胞老化のダイナミクスを評価する。

  2. Improvement of temporal resolution of scanning ion conductance microscopy using machine learning

    Grant number：20K15309  2020.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    Ida Hiroki

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    Visualization of nanoscale structures on living cell membranes allows for further understanding the details of cellular mechanisms. Scanning ion conductance microscopy (SICM) is a visualization technique for sub-micrometer structures on cell membranes. To obtain further information of rapid cellular reaction at nanoscale, it was aimed to improve temporal resolution of SICM using machine learning.
    To collect large amount of training data needed for machine learning, a new SICM system was developed for long-term measurements. Moreover, high quality images for machine learning could be obtained using this system. Also, a program was developed to convert various sets of SICM data into training data.

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Industrial property rights 1

  1. 生体分子の評価方法および生体分子の評価用装置

    井田大貴

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    Applicant：国立大学法人東海国立大学機構

    Application no：C20230586  Date applied：2024

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