Updated on 2024/04/02

写真a

 
IDA Hiroki
 
Organization
Graduate School of Engineering Electronics 2 Lecturer
Graduate School
Graduate School of Engineering
Undergraduate School
School of Engineering Electrical Engineering, Electronics, and Information Engineering
Title
Lecturer
External link

Degree 3

  1. 博士（学術） （ 2019.3   東北大学 ） 

  2. 修士（学術） （ 2016.3   東北大学 ） 

  3. 学士（工学） （ 2014.3   東北大学 ） 

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Research Interests 1

  1. scanning ion conductance microscopy

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Research Areas 1

  1. Nanotechnology/Materials / Nanobioscience

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Papers 1

  1. A noncanonical endocytic pathway is involved in the internalization of 3 μm polystyrene beads into HeLa cells. Reviewed International journal

    Hisaaki Hirose, Masashi Maekawa, Hiroki Ida, Masashi Kuriyama, Yasufumi Takahashi, Shiroh Futaki

    Biomaterials science   Vol. 10 ( 24 ) page： 7093 - 7102   2022.12

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    Language：English   Publishing type：Research paper (scientific journal)  

    Extracellular fine particles of various sizes and origins can be taken up by cells, affecting their function. Understanding the cellular uptake processes is crucial for understanding the cellular effects of these particles and the development of means to control their internalization. Although macropinocytosis is a possible pathway for the cellular uptake of particles larger than 0.2 μm, its contribution to cellular uptake in non-phagocytic cells is controversial. Using 3 μm polystyrene beads as a model particle, we aimed to assess the detailed modes of their cellular uptake by non-phagocytic HeLa cells. Cellular uptake was assessed using confocal, scanning electron, and scanning ion conductance microscopy analyses, together with inhibitor studies. Our results revealed that 3 μm beads were taken up by HeLa cells by an actin-, cholesterol-, and membrane protrusions-dependent noncanonical endocytic pathway, different from the canonical macropinocytic and phagocytic pathways. Our work provides a framework for studying the cellular uptake of extracellular fine particles.

    DOI： 10.1039/d2bm01353c

    Web of Science

    Scopus

    PubMed

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KAKENHI (Grants-in-Aid for Scientific Research) 2

  1. 細胞老化評価のための長期ナノ動態計測システムの創出

    Grant number：22K14703  2022.4 - 2024.3

    科学研究費助成事業  若手研究

    井田 大貴

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    Authorship：Principal investigator 

    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

    細胞は一般的な光学顕微鏡では観察できないナノメートルレベルでの変化が連続的に生じているが、ひとつの細胞のナノ動態を長期的に観察する事は難しかった。本研究では、非侵襲かつナノスケールで細胞表面形状を取得可能な走査型イオンコンダクタンス顕微鏡による計測を細胞培養環境下で実行可能な新規装置系を開発する。また、開発した装置を用いて数日スパンで形態や代謝活性等が連続的に変化する細胞老化のダイナミクスを評価する。

  2. Improvement of temporal resolution of scanning ion conductance microscopy using machine learning

    Grant number：20K15309  2020.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    Ida Hiroki

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    Visualization of nanoscale structures on living cell membranes allows for further understanding the details of cellular mechanisms. Scanning ion conductance microscopy (SICM) is a visualization technique for sub-micrometer structures on cell membranes. To obtain further information of rapid cellular reaction at nanoscale, it was aimed to improve temporal resolution of SICM using machine learning.
    To collect large amount of training data needed for machine learning, a new SICM system was developed for long-term measurements. Moreover, high quality images for machine learning could be obtained using this system. Also, a program was developed to convert various sets of SICM data into training data.

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