Updated on 2023/09/28


IDA Hiroki
Graduate School of Engineering Electronics 2 Lecturer
Graduate School
Graduate School of Engineering
Undergraduate School
School of Engineering Electrical Engineering, Electronics, and Information Engineering
External link

Degree 3

  1. 博士(学術) ( 2019.3   東北大学 ) 

  2. 修士(学術) ( 2016.3   東北大学 ) 

  3. 学士(工学) ( 2014.3   東北大学 ) 

Research Interests 1

  1. scanning ion conductance microscopy

Research Areas 1

  1. Nanotechnology/Materials / Nanobioscience


Papers 5

  1. In situ visualization of LbL-assembled film nanoscale morphology using scanning ion conductance microscopy

    Honda K., Yoshida K., Sato K., Ida H., Takahashi Y.

    Electrochimica Acta   Vol. 469   2023.11

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    Publisher:Electrochimica Acta  

    The layer-by-layer (LbL) technique is a simple method employed to fabricate multi-layered thin films by alternately adsorbing anionic and cationic polyelectrolytes through the electrostatic interactions. In order to fabricate thin films with desirable structures and properties, it is crucial to understand how different fabricate conditions direct the thin film formation. In this study, we performed the In situ characterization of thin firm using scanning ion conductance microscopy (SICM), where the thickness as well as the surface roughness are evaluated for films with different layer numbers. Moreover, the effects of the pH and ionic strength on the fabrication of the thin films were investigated. SICM enabled the determination of the film thickness with a single layer level and the comprehension of the relationship between the surface morphology and fabrication conditions.

    DOI: 10.1016/j.electacta.2023.143152


  2. Electrochemical imaging correlated to hydrogen evolution reaction on transition metal dichalcogenide, WS2

    Akichika Kumatani, Hiroto Ogawa, Takahiko Endo, Yu Kobayashi, Jana Lustikova, Hiroki Ida, Yasufumi Takahashi, Tomokazu Matsue, Yasumitsu Miyata, Hitoshi Shiku

    Journal of Vacuum Science & Technology B   Vol. 41 ( 5 )   2023.9

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1116/6.0002706

    Web of Science



  3. Development of High-Speed Scanning Ion Conductance Microscopy for Super Resolution Topographic Imaging of Dynamic Structural Changes of Live Cells

    Honda Kota, Ida Hiroki, Takahashi Yasufumi

    KENBIKYO   Vol. 58 ( 2 ) page: 71 - 75   2023.8

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    Language:Japanese   Publisher:The Japanese Society of Microscopy  

    <p>Visualization of the dynamics on the living cell surface is essential for understanding cellular functions such as migration, cancer invasion, and differentiation. In order to evaluate cellular dynamics, it is necessary to develop visualization techniques with nanoscale spatial resolution and non-invasion. Scanning ion conductance microscopy (SICM) is one of the most powerful microscopy techniques for obtaining fragile cell morphology under physiological conditions. In recent years, several research groups have improved the temporal resolution of SICM to visualize rapid cellular reactions. Here, we provide an overview of SICM, including the basic scanning mode and equipment, and recent researches on the development of high-speed SICM.</p>

    DOI: 10.11410/kenbikyo.58.2_71

    CiNii Research

  4. Nanopipette Fabrication Guidelines for SICM Nanoscale Imaging. International journal

    Yasufumi Takahashi, Yuya Sasaki, Takeshi Yoshida, Kota Honda, Yuanshu Zhou, Takafumi Miyamoto, Tomoko Motoo, Hiroki Higashi, Andrew Shevchuk, Yuri Korchev, Hiroki Ida, Rikinari Hanayama, Takeshi Fukuma

    Analytical chemistry   Vol. 95 ( 34 ) page: 12664 - 12672   2023.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    Scanning ion conductance microscopy (SICM) is a promising tool for visualizing the dynamics of nanoscale cell surface topography. However, there are still no guidelines for fabricating nanopipettes with ideal shape consisting of small apertures and thin glass walls. Therefore, most of the SICM imaging has been at a standstill at the submicron scale. In this study, we established a simple and highly reproducible method for the fabrication of nanopipettes with sub-20 nm apertures. To validate the improvement in the spatial resolution, we performed time-lapse imaging of the formation and disappearance of endocytic pits as a model of nanoscale time-lapse topographic imaging. We have also successfully imaged the localization of the hot spot and the released extracellular vesicles. The nanopipette fabrication guidelines for the SICM nanoscale topographic imaging can be an essential tool for understanding cell-cell communication.

    DOI: 10.1021/acs.analchem.3c01010

    Web of Science




  5. A noncanonical endocytic pathway is involved in the internalization of 3 μm polystyrene beads into HeLa cells. Reviewed International journal

    Hisaaki Hirose, Masashi Maekawa, Hiroki Ida, Masashi Kuriyama, Yasufumi Takahashi, Shiroh Futaki

    Biomaterials science   Vol. 10 ( 24 ) page: 7093 - 7102   2022.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    Extracellular fine particles of various sizes and origins can be taken up by cells, affecting their function. Understanding the cellular uptake processes is crucial for understanding the cellular effects of these particles and the development of means to control their internalization. Although macropinocytosis is a possible pathway for the cellular uptake of particles larger than 0.2 μm, its contribution to cellular uptake in non-phagocytic cells is controversial. Using 3 μm polystyrene beads as a model particle, we aimed to assess the detailed modes of their cellular uptake by non-phagocytic HeLa cells. Cellular uptake was assessed using confocal, scanning electron, and scanning ion conductance microscopy analyses, together with inhibitor studies. Our results revealed that 3 μm beads were taken up by HeLa cells by an actin-, cholesterol-, and membrane protrusions-dependent noncanonical endocytic pathway, different from the canonical macropinocytic and phagocytic pathways. Our work provides a framework for studying the cellular uptake of extracellular fine particles.

    DOI: 10.1039/d2bm01353c

    Web of Science




KAKENHI (Grants-in-Aid for Scientific Research) 1

  1. 細胞老化評価のための長期ナノ動態計測システムの創出

    Grant number:22K14703  2022.4 - 2024.3

    科学研究費助成事業  若手研究

    井田 大貴

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    Authorship:Principal investigator 

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )