Updated on 2024/04/03

写真a

 
TSUJI Hiroyuki
 
Organization
Bioscience and Biotechnology Center Professor
Graduate School
Graduate School of Bioagricultural Sciences
Title
Professor
Profile
私たちは、花をつくる最強の運命決定因子・フロリゲンの分子機能解明と植物改良への展開をめざして研究しています。生命科学の最先端の研究技術を基軸にして、これまで誰も見たことのないものを見る。フロリゲンの研究を通して、生命科学の新しい領域を切り開く研究を進めます。
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Degree 1

  1. 博士(農学) ( 2004.3   東京大学 ) 

Research Interests 8

  1. フロリゲン

  2. 茎頂メリステム

  3. 花成

  4. イネ

  5. オオムギ

  6. コムギ

  7. 発生学

  8. フロリゲン活性化複合体

Research Areas 1

  1. Environmental Science/Agriculture Science / Science in plant genetics and breeding

Current Research Project and SDGs 1

  1. フロリゲンの分子機能解明と植物改良への展開

Research History 8

  1. Nagoya University   Professor

    2022.4

  2. Yokohama City University   Kihara Institute for Biological Research   Professor

    2024.4

  3. Yokohama City University   Associate professor

    2016.4

  4. Yokohama City University   Lecturer

    2015.4 - 2016.3

  5. Nara Institute of Science and Technology   Assistant Professor

    2006.4 - 2015.3

  6. Japan Society for Promotion of Science

    2005.4 - 2006.3

  7. Nagoya University   Researcher

    2004.4 - 2005.3

  8. Japan Society for Promotion of Science

    2001.4 - 2004.3

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Education 3

  1. The University of Tokyo   Graduate School of Agricultural and Life Sciences

    2001.4 - 2004.3

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  2. The University of Tokyo

    1999.4 - 2001.3

  3. The University of Tokyo

    1995.4 - 1999.3

Professional Memberships 4

  1. 日本育種学会   シンポジウム委員

    2024.3

  2. 日本植物生理学会   広報委員

    2024.3

  3. 日本育種学会   編集幹事

    2020.4 - 2024.3

  4. 日本植物生理学会   庶務幹事

    2017.3 - 2019.3

Committee Memberships 6

  1. 日本育種学会   シンポジウム委員  

    2024.3   

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    Committee type:Academic society

  2. 日本植物生理学会   広報委員  

    2024.3   

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    Committee type:Academic society

  3. Breeding Science   Editor  

    2021.4   

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  4. Japanese Society of Breeding   Managing Editor  

    2020.4 - 2024.3   

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    Committee type:Academic society

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  5. Rice (Springer Journal)   Editor  

    2017.4   

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  6. 日本植物生理学会   庶務幹事  

    2017.3 - 2019.3   

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Awards 2

  1. 日本育種学会 奨励賞

    2015.3   日本育種学会   花成ホルモン・フロリゲンの機能に関する遺伝育種学的研究

    辻 寛之

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  2. 日本育種学会 奨励賞

    2015.3   日本育種学会   花成ホルモン・フロリゲンの機能に関する遺伝育種学的研究

    辻 寛之

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Papers 61

  1. DNA methylation is reconfigured at the onset of reproduction in rice shoot apical meristem. Reviewed International journal

    Asuka Higo, Noriko Saihara, Fumihito Miura, Yoko Higashi, Megumi Yamada, Shojiro Tamaki, Tasuku Ito, Yoshiaki Tarutani, Tomoaki Sakamoto, Masayuki Fujiwara, Tetsuya Kurata, Yoichiro Fukao, Satoru Moritoh, Rie Terada, Toshinori Kinoshita, Takashi Ito, Tetsuji Kakutani, Ko Shimamoto, Hiroyuki Tsuji* (Corresponding Author)

    Nature Communications   Vol. 11 ( 1 ) page: 4079 - 4079   2020.8

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    DNA methylation is an epigenetic modification that specifies the basic state of pluripotent stem cells and regulates the developmental transition from stem cells to various cell types. In flowering plants, the shoot apical meristem (SAM) contains a pluripotent stem cell population which generates the aerial part of plants including the germ cells. Under appropriate conditions, the SAM undergoes a developmental transition from a leaf-forming vegetative SAM to an inflorescence- and flower-forming reproductive SAM. While SAM characteristics are largely altered in this transition, the complete picture of DNA methylation remains elusive. Here, by analyzing whole-genome DNA methylation of isolated rice SAMs in the vegetative and reproductive stages, we show that methylation at CHH sites is kept high, particularly at transposable elements (TEs), in the vegetative SAM relative to the differentiated leaf, and increases in the reproductive SAM via the RNA-dependent DNA methylation pathway. We also show that half of the TEs that were highly methylated in gametes had already undergone CHH hypermethylation in the SAM. Our results indicate that changes in DNA methylation begin in the SAM long before germ cell differentiation to protect the genome from harmful TEs.

    DOI: 10.1038/s41467-020-17963-2

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  2. Multiple wheat genomes reveal global variation in modern breeding. Reviewed International journal

    Sean Walkowiak, Liangliang Gao, Cecile Monat, Georg Haberer, Mulualem T Kassa, Jemima Brinton, Ricardo H Ramirez-Gonzalez, Markus C Kolodziej, Emily Delorean, Dinushika Thambugala, Valentyna Klymiuk, Brook Byrns, Heidrun Gundlach, Venkat Bandi, Jorge Nunez Siri, Kirby Nilsen, Catharine Aquino, Axel Himmelbach, Dario Copetti, Tomohiro Ban, Luca Venturini, Michael Bevan, Bernardo Clavijo, Dal-Hoe Koo, Jennifer Ens, Krystalee Wiebe, Amidou N'Diaye, Allen K Fritz, Carl Gutwin, Anne Fiebig, Christine Fosker, Bin Xiao Fu, Gonzalo Garcia Accinelli, Keith A Gardner, Nick Fradgley, Juan Gutierrez-Gonzalez, Gwyneth Halstead-Nussloch, Masaomi Hatakeyama, Chu Shin Koh, Jasline Deek, Alejandro C Costamagna, Pierre Fobert, Darren Heavens, Hiroyuki Kanamori, Kanako Kawaura, Fuminori Kobayashi, Ksenia Krasileva, Tony Kuo, Neil McKenzie, Kazuki Murata, Yusuke Nabeka, Timothy Paape, Sudharsan Padmarasu, Lawrence Percival-Alwyn, Sateesh Kagale, Uwe Scholz, Jun Sese, Philomin Juliana, Ravi Singh, Rie Shimizu-Inatsugi, David Swarbreck, James Cockram, Hikmet Budak, Toshiaki Tameshige, Tsuyoshi Tanaka, Hiroyuki Tsuji, Jonathan Wright, Jianzhong Wu, Burkhard Steuernagel, Ian Small, Sylvie Cloutier, Gabriel Keeble-Gagnère, Gary Muehlbauer, Josquin Tibbets, Shuhei Nasuda, Joanna Melonek, Pierre J Hucl, Andrew G Sharpe, Matthew Clark, Erik Legg, Arvind Bharti, Peter Langridge, Anthony Hall, Cristobal Uauy, Martin Mascher, Simon G Krattinger, Hirokazu Handa, Kentaro K Shimizu, Assaf Distelfeld, Ken Chalmers, Beat Keller, Klaus F X Mayer, Jesse Poland, Nils Stein, Curt A McCartney, Manuel Spannagl, Thomas Wicker, Curtis J Pozniak

    Nature   Vol. 588 ( 7837 ) page: 277 - 283   2020.12

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    Advances in genomics have expedited the improvement of several agriculturally important crops but similar efforts in wheat (Triticum spp.) have been more challenging. This is largely owing to the size and complexity of the wheat genome1, and the lack of genome-assembly data for multiple wheat lines2,3. Here we generated ten chromosome pseudomolecule and five scaffold assemblies of hexaploid wheat to explore the genomic diversity among wheat lines from global breeding programs. Comparative analysis revealed extensive structural rearrangements, introgressions from wild relatives and differences in gene content resulting from complex breeding histories aimed at improving adaptation to diverse environments, grain yield and quality, and resistance to stresses4,5. We provide examples outlining the utility of these genomes, including a detailed multi-genome-derived nucleotide-binding leucine-rich repeat protein repertoire involved in disease resistance and the characterization of Sm16, a gene associated with insect resistance. These genome assemblies will provide a basis for functional gene discovery and breeding to deliver the next generation of modern wheat cultivars.

    DOI: 10.1038/s41586-020-2961-x

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  3. Antagonistic regulation of the gibberellic acid response during stem growth in rice. Reviewed International journal

    Keisuke Nagai, Yoshinao Mori, Shin Ishikawa, Tomoyuki Furuta, Rico Gamuyao, Yoko Niimi, Tokunori Hobo, Moyuri Fukuda, Mikiko Kojima, Yumiko Takebayashi, Atsushi Fukushima, Yasuyo Himuro, Masatomo Kobayashi, Wataru Ackley, Hiroshi Hisano, Kazuhiro Sato, Aya Yoshida, Jianzhong Wu, Hitoshi Sakakibara, Yutaka Sato, Hiroyuki Tsuji, Takashi Akagi, Motoyuki Ashikari

    Nature   Vol. 584 ( 7819 ) page: 109 - +   2020.8

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    The size of plants is largely determined by growth of the stem. Stem elongation is stimulated by gibberellic acid1-3. Here we show that internode stem elongation in rice is regulated antagonistically by an 'accelerator' and a 'decelerator' in concert with gibberellic acid. Expression of a gene we name ACCELERATOR OF INTERNODE ELONGATION 1 (ACE1), which encodes a protein of unknown function, confers cells of the intercalary meristematic region with the competence for cell division, leading to internode elongation in the presence of gibberellic acid. By contrast, upregulation of DECELERATOR OF INTERNODE ELONGATION 1 (DEC1), which encodes a zinc-finger transcription factor, suppresses internode elongation, whereas downregulation of DEC1 allows internode elongation. We also show that the mechanism of internode elongation that is mediated by ACE1 and DEC1 is conserved in the Gramineae family. Furthermore, an analysis of genetic diversity suggests that mutations in ACE1 and DEC1 have historically contributed to the selection of shorter plants in domesticated populations of rice to increase their resistance to lodging, and of taller plants in wild species of rice for adaptation to growth in deep water. Our identification of these antagonistic regulatory factors enhances our understanding of the gibberellic acid response as an additional mechanism that regulates internode elongation and environmental fitness, beyond biosynthesis and gibberellic acid signal transduction.

    DOI: 10.1038/s41586-020-2501-8

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  4. 14-3-3 proteins act as intracellular receptors for rice Hd3a florigen Reviewed International coauthorship

    Ken-ichiro Taoka*, Izuru Ohki*, Hiroyuki Tsuji* (*co-first authors), Kyoko Furuita, Kokoro Hayashi, Tomoko Yanase, Midori Yamaguchi, Chika Nakashima, Yekti Asih Purwestri, Shojiro Tamaki, Yuka Ogaki, Chihiro Shimada, Atsushi Nakagawa, Chojiro Kojima, Ko Shimamoto

    Nature   Vol. 476 ( 7360 ) page: 332 - U97   2011.8

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    'Florigen' was proposed 75 years ago(1) to be synthesized in the leaf and transported to the shoot apex, where it induces flowering. Only recently have genetic and biochemical studies established that florigen is encoded by FLOWERING LOCUS T (FT), a gene that is universally conserved in higher plants(2-4). Nonetheless, the exact function of florigen during floral induction remains poorly understood and receptors for florigen have not been identified. Here we show that the rice FT homologue Hd3a(5) interacts with 14-3-3 proteins in the apical cells of shoots, yielding a complex that translocates to the nucleus and binds to the Oryza sativa (Os)FD1 transcription factor, a rice homologue of Arabidopsis thaliana FD. The resultant ternary 'florigen activation complex' (FAC) induces transcription of OsMADS15, a homologue of A. thaliana APETALA1 (AP1), which leads to flowering. We have determined the 2.4 angstrom crystal structure of rice FAC, which provides a mechanistic basis for florigen function in flowering. Our results indicate that 14-3-3 proteins act as intracellular receptors for florigen in shoot apical cells, and offer new approaches to manipulate flowering in various crops and trees.

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  5. FT-like proteins induce transposon silencing in the shoot apex during floral induction in rice Reviewed

    Shojiro Tamaki, Hiroyuki Tsuji* (*Corresponding Author), Ayana Matsumoto, Akiko Fujita, Zenpei Shimatani, Rie Terada, Tomoaki Sakamoto, Tetsuya Kurata, Ko Shimamoto

    PNAS   Vol. 112 ( 8 ) page: E901 - E910   2015.2

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    Floral induction is a crucial developmental step in higher plants. Florigen, a mobile floral activator that is synthesized in the leaf and transported to the shoot apex, was recently identified as a protein encoded by FLOWERING LOCUS T (FT) and its orthologs; the rice florigen is Heading date 3a (Hd3a) protein. The 14-3-3 proteins mediate the interaction of Hd3a with the transcription factor OsFD1 to form a ternary structure called the florigen activation complex on the promoter of OsMADS15, a rice APETALA1 ortholog. However, crucial information, including the spatiotemporal overlap among FT-like proteins and the components of florigen activation complex and downstream genes, remains unclear. Here, we confirm that Hd3a coexists, in the same regions of the rice shoot apex, with the other components of the florigen activation complex and its transcriptional targets. Unexpectedly, however, RNA-sequencing analysis of shoot apex from wild-type and RNA-interference plants depleted of florigen activity revealed that 4,379 transposable elements (TEs; 58% of all classifiable rice TEs) were expressed collectively in the vegetative and reproductive shoot apex. Furthermore, in the reproductive shoot apex, 214 TEs were silenced by florigen. Our results suggest a link between floral induction and regulation of TEs.

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  6. Dual role of tree florigen activation complex component FD in photoperiodic growth control and adaptive response pathways Reviewed

    Szymon Tylewicz*, Hiroyuki Tsuji* (*co-first author), Pal Miskolczi, Anna Petterle, Abdul Azeez, Kristoffer Jonsson, Ko Shimamoto, Rishikesh P. Bhalerao

    PNAS   Vol. 112 ( 10 ) page: 3140 - 3145   2015.3

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    A complex consisting of evolutionarily conserved FD, FLOWERING LOCUS T (FT) proteins is a regulator of floral transition. Intriguingly, FT orthologs are also implicated in developmental transitions distinct from flowering, such as photoperiodic control of bulbing in onions, potato tuberization, and growth cessation in trees. However, whether an FT-FD complex participates in these transitions and, if so, its mode of action, are unknown. We identified two closely related FD homologs, FD-like 1 (FDL1) and FD-like 2 (FDL2), in the model tree hybrid aspen. Using gain of function and RNAi-suppressed FDL1 and FDL2 transgenic plants, we show that FDL1 and FDL2 have distinct functions and a complex consisting of FT and FDL1 mediates in photoperiodic control of seasonal growth. The downstream target of the FT-FD complex in photoperiodic control of growth is Like AP1 (LAP1), a tree ortholog of the floral meristem identity gene APETALA1. Intriguingly, FDL1 also participates in the transcriptional control of adaptive response and bud maturation pathways, independent of its interaction with FT, presumably via interaction with ABSCISIC ACID INSENSITIVE 3 (ABI3) transcription factor, a component of abscisic acid (ABA) signaling. Our data reveal that in contrast to its primary role in flowering, FD has dual roles in the photoperiodic control of seasonal growth and stress tolerance in trees. Thus, the functions of FT and FD have diversified during evolution, and FD homologs have acquired roles that are independent of their interaction with FT.

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  7. A leaf-emanated signal orchestrates grain size and number in response to maternal resources Reviewed

    Ta, K.N., Shimizu-Sato, S., Agata, A., Yoshida, Y., Taoka, K.-i., Tsuji, H., Akagi, T., Tanizawa, Y., Sano, R., Nosaka-Takahashi, M., Suzuki, T., Demura, T., Toyoda, A., Nakamura, Y. and Sato, Y.

    Plant J.   Vol. 115   page: 175 - 189   2023

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  8. MORE PANICLES 3, a natural allele of OsTB1/FC1, impacts rice yield in paddy fields at elevated CO2 levels Reviewed

    Takai, T., Taniguchi, Y., Takahashi, M., Nagasaki, H., Yamamoto, E., Hirose, S., Hara, N., Akashi, H., Ito, J., Arai-Sanoh, Y., Hori, K., Fukuoka, S., Sakai, H., Tokida, T., Usui, Y., Nakamura, H., Kawamura, K., Asai, H., Ishizaki, T., Maruyama, K., Mochida, K., Kobayashi, N., Kondo, M., Tsuji, H., Tsujimoto, Y., Hasegawa, T., Uga, Y.

    Plant J.   Vol. 114   page: 729 - 742   2023

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  9. Molecular and cellular insights into auxin-regulated primary root growth: a comparative study of Arabidopsis and rice Invited Reviewed

    Nakamura, A., Hirota, Y., Shigihara, M., Watanabe, M., Sato, A., Tsuji, H., Shimada, Y.

      Vol. 87   page: 1145 - 1154   2023

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  10. Multifunctional chemical inhibitors of the florigen activation complex discovered by structure-based high-throughput screening. Reviewed International journal

    Ken-Ichiro Taoka, Ikumi Kawahara, Shoko Shinya, Ken-Ichi Harada, Eiki Yamashita, Zenpei Shimatani, Kyoko Furuita, Tomoaki Muranaka, Tokitaka Oyama, Rie Terada, Atsushi Nakagawa, Toshimichi Fujiwara, Hiroyuki Tsuji, Chojiro Kojima

    The Plant journal : for cell and molecular biology   Vol. 112 ( 6 ) page: 1337 - 1349   2022.12

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    Structure-based high-throughput screening of chemical compounds that target protein-protein interactions (PPI) is a promising technology for gaining insight into how plant development is regulated, leading to many potential agricultural applications. At present, there are no examples of using high-throughput screening to identify chemicals that target plant transcriptional complexes, some of which are responsible for regulating multiple physiological functions. Florigen, a protein encoded by FLOWERING LOCUS T (FT), was initially identified as molecule that promotes flowering and has since been shown to regulate flowering and other developmental phenomena such as tuber formation in potato. FT functions as a component of the florigen activation complex (FAC) with a scaffold protein 14-3-3 and FD, a bZIP transcription factor that activates downstream gene expression. Although 14-3-3 is an important component of FAC, there are little functional analysis of the 14-3-3 itself. Here, we report the results of a high-throughput in vitro fluorescence resonance energy transfer (FRET) screening of chemical libraries that enabled us to identify small molecules capable of inhibiting FAC formation. These molecules abrogate the in vitro interaction between 14-3-3 and OsFD1 peptide, a rice FD, by directly binding to 14-3-3. Treatment with S4, a specific hit molecule, strongly inhibited FAC activity and flowering in duckweed, tuber formation in potato and branching in rice in a dose-dependent manner. Our results demonstrate that the high-throughput screening approach based on three-dimensional structure of PPI is possible in plants. In this study, we have proposed good candidate compounds for future modification to obtain inhibitors of florigen-dependent processes through inhibition of FAC formation.

    DOI: 10.1111/tpj.16008

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  11. Crystal structure of potato 14-3-3 protein St14f revealed the importance of helix I in StFDL1 recognition. Reviewed International journal

    Ken-Ichi Harada, Kyoko Furuita, Eiki Yamashita, Ken-Ichiro Taoka, Hiroyuki Tsuji, Toshimichi Fujiwara, Atsushi Nakagawa, Chojiro Kojima

    Scientific reports   Vol. 12 ( 1 ) page: 11596 - 11596   2022.7

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    In potato (Solanum tuberosum L.), 14-3-3 protein forms a protein complex with the FLOWERING LOCUS T (FT)-like protein StSP6A and the FD-like protein StFDL1 to activate potato tuber formation. Eleven 14-3-3 isoforms were reported in potato, designated as St14a-k. In this study, the crystal structure of the free form of St14f was determined at 2.5 Å resolution. Three chains were included in the asymmetric unit of the St14f free form crystal, and the structural deviation among the three chain structures was found on the C-terminal helix H and I. The St14f free form structure in solution was also investigated by nuclear magnetic resonance (NMR) residual dipolar coupling analysis, and the chain B in the crystal structure was consistent with NMR data. Compared to other crystal structures, St14f helix I exhibited a different conformation with larger B-factor values. Larger B-factor values on helix I were also found in the 14-3-3 free form structure with higher solvent contents. The mutation in St14f Helix I stabilized the complex with StFDL1. These data clearly showed that the flexibility of helix I of 14-3-3 protein plays an important role in the recognition of target protein.

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  12. Auxin Distribution in Lateral Root Primordium Development Affects the Size and Lateral Root Diameter of Rice Reviewed

    Tsubasa Kawai, Ryosuke Akahoshi, Israt J. Shelley, Takaaki Kojima, Moeko Sato, Hiroyuki Tsuji, Yoshiaki Inukai

    Frontiers in Plant Science   Vol. 13   page: 834378   2022.4

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    Lateral roots (LRs) occupy a large part of the root system and play a central role in plant water and nutrient uptake. Monocot plants, such as rice, produce two types of LRs: the S-type (short and thin) and the L-type (long, thick, and capable of further branching). Because of the ability to produce higher-order branches, the L-type LR formation contributes to efficient root system expansion. Auxin plays a major role in regulating the root system development, but its involvement in developing different types of LRs is largely unknown. Here, we show that auxin distribution is involved in regulating LR diameter. Dynamin-related protein (DRP) genes were isolated as causative genes of the mutants with increased L-type LR number and diameter than wild-type (WT). In the drp mutants, reduced endocytic activity was detected in rice protoplast and LRs with a decreased OsPIN1b-GFP endocytosis in the protoplast. Analysis of auxin distribution using auxin-responsive promoter DR5 revealed the upregulated auxin signaling in L-type LR primordia (LRP) of the WT and the mutants. The application of polar auxin transport inhibitors enhanced the effect of exogenous auxin to increase LR diameter with upregulated auxin signaling in the basal part of LRP. Inducible repression of auxin signaling in the mOsIAA3-GR system suppressed the increase in LR diameter after root tip excision, suggesting a positive role of auxin signaling in LR diameter increase. A positive regulator of LR diameter, OsWOX10, was auxin-inducible and upregulated in the drp mutants more than the WT, and revealed as a potential target of ARF transcriptional activator. Therefore, auxin signaling upregulation in LRP, especially at the basal part, induces OsWOX10 expression, increasing LR diameter.

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  13. Improved clearing method contributes to deep imaging of plant organs. Reviewed International journal

    Yuki Sakamoto, Anna Ishimoto, Yuuki Sakai, Moeko Sato, Ryuichi Nishihama, Konami Abe, Yoshitake Sano, Teiichi Furuichi, Hiroyuki Tsuji, Takayuki Kohchi, Sachihiro Matsunaga

    Communications biology   Vol. 5 ( 1 ) page: 12 - 12   2022.1

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    Tissue clearing methods are increasingly essential for the microscopic observation of internal tissues of thick biological organs. We previously developed TOMEI, a clearing method for plant tissues; however, it could not entirely remove chlorophylls nor reduce the fluorescent signal of fluorescent proteins. Here, we developed an improved TOMEI method (iTOMEI) to overcome these limitations. First, a caprylyl sulfobetaine was determined to efficiently remove chlorophylls from Arabidopsis thaliana seedlings without GFP quenching. Next, a weak alkaline solution restored GFP fluorescence, which was mainly lost during fixation, and an iohexol solution with a high refractive index increased sample transparency. These procedures were integrated to form iTOMEI. iTOMEI enables the detection of much brighter fluorescence than previous methods in tissues of A. thaliana, Oryza sativa, and Marchantia polymorpha. Moreover, a mouse brain was also efficiently cleared by the iTOMEI-Brain method within 48 h, and strong fluorescent signals were detected in the cleared brain.

    DOI: 10.1038/s42003-021-02955-9

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  14. Whole-Tissue Three-Dimensional Imaging of Rice at Single-Cell Resolution. Reviewed International journal

    Moeko Sato, Hiroko Akashi, Yuki Sakamoto, Sachihiro Matsunaga, Hiroyuki Tsuji

    International journal of molecular sciences   Vol. 23 ( 1 )   2022.1

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    The three-dimensional (3D) arrangement of cells in tissues provides an anatomical basis for analyzing physiological and biochemical aspects of plant and animal cellular development and function. In this study, we established a protocol for tissue clearing and 3D imaging in rice. Our protocol is based on three improvements: clearing with iTOMEI (clearing solution suitable for plants), developing microscopic conditions in which the Z step is optimized for 3D reconstruction, and optimizing cell-wall staining. Our protocol successfully 3D imaged rice shoot apical meristems, florets, and root apical meristems at cellular resolution throughout whole tissues. Using fluorescent reporters of auxin signaling in rice root tips, we also revealed the 3D distribution of auxin signaling events that are activated in the columella, quiescent center, and multiple rows of cells in the stele of the root apical meristem. Examination of cells with higher levels of auxin signaling revealed that only the central row of cells was connected to the quiescent center. Our method provides opportunities to observe the 3D arrangement of cells in rice tissues.

    DOI: 10.3390/ijms23010040

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  15. Regulatory functions of ROS dynamics via glutathione metabolism and glutathione peroxidase activity in developing rice zygote. International journal

    Kasidit Rattanawong, Narumi Koiso, Erika Toda, Atsuko Kinoshita, Mari Tanaka, Hiroyuki Tsuji, Takashi Okamoto

    The Plant journal : for cell and molecular biology   Vol. 108 ( 4 ) page: 1097 - 1115   2021.11

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    Reactive oxygen species (ROS) play essential roles in plant development and environmental stress responses. In this study, ROS dynamics, the glutathione redox status, the expression and subcellular localization of glutathione peroxidases (GPXs), and the effects of inhibitors of ROS-mediated metabolism were investigated along with fertilization and early zygotic embryogenesis in rice (Oryza sativa). Zygotes and early embryos exhibited developmental arrest upon inhibition of ROS production. Egg cells accumulated high ROS levels, and, after fertilization, intracellular ROS levels progressively declined in zygotes in which de novo expression of GPX1 and 3 was observed through upregulation of the genes. In addition to inhibition of GPX activity, depletion of glutathione impeded early embryonic development and led to failure of the zygote to appropriately decrease H2 O2 levels. Moreover, through monitoring of the glutathione redox status, the developing zygotes exhibited a progressive glutathione oxidation, which became extremely delayed under inhibited GPX activity. Our results provide insights into the importance of ROS dynamics, GPX antioxidant activity, and glutathione redox metabolism during zygotic/embryonic development.

    DOI: 10.1111/tpj.15497

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  16. Characterization of Frond and Flower Development and Identification of FT and FD Genes From Duckweed Lemna aequinoctialis Nd. International journal

    Akiko Yoshida, Ken-Ichiro Taoka, Aoi Hosaka, Keisuke Tanaka, Hisato Kobayashi, Tomoaki Muranaka, Kiminori Toyooka, Tokitaka Oyama, Hiroyuki Tsuji

    Frontiers in plant science   Vol. 12   page: 697206 - 697206   2021.10

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    Duckweeds (Araceae: Lemnoideae) are aquatic monocotyledonous plants that are characterized by their small size, rapid growth, and wide distribution. Developmental processes regulating the formation of their small leaf-like structures, called fronds, and tiny flowers are not well characterized. In many plant species, flowering is promoted by the florigen activation complex, whose major components are florigen FLOWERING LOCUS T (FT) protein and transcription factor FD protein. How this complex is regulated at the molecular level during duckweed flowering is also not well understood. In this study, we characterized the course of developmental changes during frond development and flower formation in Lemna aequinoctialis Nd, a short-day plant. Detailed observations of frond and flower development revealed that cell proliferation in the early stages of frond development is active as can be seen in the separate regions corresponding to two budding pouches in the proximal region of the mother frond. L. aequinoctialis produces two stamens of different lengths with the longer stamen growing more rapidly. Using high-throughput RNA sequencing (RNA-seq) and de novo assembly of transcripts from plants induced to flower, we identified the L. aequinoctialis FT and FD genes, whose products in other angiosperms form a transcriptional complex to promote flowering. We characterized the protein-protein interaction of duckweed FT and FD in yeast and examined the functions of the two gene products by overexpression in Arabidopsis. We found that L. aequinoctialis FTL1 promotes flowering, whereas FTL2 suppresses flowering.

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  17. Mutation of OUR1/OsbZIP1, which encodes a member of the basic leucine zipper transcription factor family, promotes root development in rice through repressing auxin signaling International journal

    Tomomi Hasegawa, Nonawin Lucob-Agustin, Koki Yasufuku, Takaaki Kojima, Shunsaku Nishiuchi, Atsushi Ogawa, Misuzu Takahashi-Nosaka, Mana Kano-Nakata, Mayuko Inari-Ikeda, Moeko Sato, Hiroyuki Tsuji, Cornelius Mbathi Wainaina, Akira Yamauchi, Yoshiaki Inukai

    Plant Science   Vol. 306   page: 110861 - 110861   2021.5

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    A well-developed root system is essential for efficient water uptake, particularly in drought-prone environments. However, the molecular mechanisms underlying the promotion of root development are poorly understood. We identified and characterized a rice mutant, outstanding rooting1 (our1), which exhibited a well-developed root system. The our1 mutant displayed typical auxin-related phenotypes, including elongated seminal root and defective gravitropism. Seminal root elongation in the our1 mutant was accelerated via the promotion of cell division and elongation. In addition, compared with the wild type, the density of short and thin lateral roots (S-type LRs) was reduced in the our1 mutant, whereas that of long and thick LRs (L-type LRs) was increased. Expression of OUR1, which encodes OsbZIP1, a member of the basic leucine zipper transcription factor family, was observed in the seminal root tip and sites of LR emergence, wherein attenuation of reporter gene expression levels controlled by the auxin response promoter DR5 was also observed in the our1 mutant. Taken together, our results indicate that the our1 gene promotes root development by suppressing auxin signaling, which may be a key factor contributing to an improvement in root architecture.

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  18. Novel assays to monitor gene expression and protein-protein interactions in rice using the bioluminescent protein, NanoLuc

    Taoka Ken-ichiro, Shimatani Zenpei, Yamaguchi Koji, Ogawa Mana, Saitoh Hiromi, Ikeda Yoichi, Akashi Hiroko, Terada Rie, Kawasaki Tsutomu, Tsuji Hiroyuki

    Plant Biotechnology   Vol. 38 ( 1 ) page: 89 - 99   2021.3

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    <p>Luciferases have been widely utilized as sensitive reporters to monitor gene expression and protein-protein interactions. Compared to firefly luciferase (Fluc), a recently developed luciferase, Nanoluciferase (NanoLuc or Nluc), has several superior properties such as a smaller size and stronger luminescence activity. We compared the reporter properties of Nluc and Fluc in rice (<i>Oryza sativa</i>). In both plant-based two-hybrid and split luc complementation (SLC) assays, Nluc activity was detected with higher sensitivity and specificity than that with Fluc. To apply Nluc to research involving the photoperiodic regulation of flowering, we made a knock-in rice plant in which the Nluc coding region was inserted in-frame with the <i>OsMADS15</i> gene, a target of the rice florigen Hd3a. Strong Nluc activity in response to Hd3a, and in response to change in day length, was detected in rice protoplasts and in a single shoot apical meristem, respectively. Our results indicate that Nluc assay systems will be powerful tools to monitor gene expression and protein-protein interaction in plant research.</p>

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  19. De Novo Genome Assembly of the Japanese Wheat Cultivar Norin 61 Highlights Functional Variation in Flowering Time and Fusarium Resistance Genes in East Asian Genotypes.

    Kentaro K Shimizu, Dario Copetti, Moeko Okada, Thomas Wicker, Toshiaki Tameshige, Masaomi Hatakeyama, Rie Shimizu-Inatsugi, Catharine Aquino, Kazusa Nishimura, Fuminori Kobayashi, Kazuki Murata, Tony Kuo, Emily Delorean, Jesse Poland, Georg Haberer, Manuel Spannagl, Klaus F X Mayer, Juan Gutierrez-Gonzalez, Gary J Muehlbauer, Cecile Monat, Axel Himmelbach, Sudharsan Padmarasu, Martin Mascher, Sean Walkowiak, Tetsuya Nakazaki, Tomohiro Ban, Kanako Kawaura, Hiroyuki Tsuji, Curtis Pozniak, Nils Stein, Jun Sese, Shuhei Nasuda, Hirokazu Handa

    Plant & Cell Physiology   Vol. 62 ( 1 ) page: 8 - 27   2021.1

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    Bread wheat is a major crop that has long been the focus of basic and breeding research. Assembly of its genome has been difficult because of its large size and allohexaploid nature (AABBDD genome). Following the first reported assembly of the genome of the experimental strain Chinese Spring (CS), the 10+ Wheat Genomes Project was launched to produce multiple assemblies of worldwide modern cultivars. The only Asian cultivar in the project is Norin 61, a representative Japanese cultivar adapted to grow across a broad latitudinal range, mostly characterized by a wet climate and a short growing season. Here, we characterize key aspects of its chromosome-scale genome assembly spanning 15 Gb with a raw scaffold N50 of 23 Mb. Analysis of the repetitive elements identified chromosomal regions unique to Norin 61 that encompass a tandem array of the pathogenesis-related-13 family. We report novel copy-number variations in the B homeolog of the florigen gene FT1/VRN3, pseudogenization of its D homeolog, and the association of its A homeologous alleles with the spring/winter growth habit. Further, the Norin 61 genome carries typical East Asian functional variants from CS ranging from a single nucleotide to multi-Mb scale. Examples of such variation are the Fhb1 locus, which confers Fusarium head-blight resistance, Ppd-D1a, which confers early flowering, Glu-D1f for Asian noodle quality, and Rht-D1b, which introduced semi-dwarfism during the green revolution. The adoption of Norin 61 as a reference assembly for functional and evolutionary studies will enable comprehensive characterization of the underexploited Asian bread wheat diversity.

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  20. Three-dimensional imaging of the shoot apex in barley Reviewed

    Ito Jun, Tsuji Hiroyuki

    PLANT MORPHOLOGY   Vol. 33 ( 1 ) page: 25 - 30   2021

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    <p>The timing of plant reproduction and inflorescence architecture have a large impact on yield of crops. Reproductive development in temperate cereals such as barley and wheat is regulated by two developmental transitions: switching of the identity in the shoot apical meristem (SAM) from vegetative to reproductive phase and conversion of the developmental fate of lateral shoot meristems generated from SAM. Although genetic studies have clarified genetic components and environmental factors that affect inflorescence architecture and its developmental process, little is known about the cellular behavior at the shoot apex including SAM and lateral primordia during development especially in Triticeae crops. We established the 3D-imaging system of barley shoot apex using a clearing method to analyze the cellular dynamics during their developmental process. In this review, we summarize the findings about the morphological characters of the shoot apex in barley and introduce our method of 3D-imaging using the shoot apex of barley.</p>

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  21. Field transcriptome analysis reveals a molecular mechanism for cassava-flowering in a mountainous environment in Southeast Asia. International journal

    Hiroki Tokunaga, Do Thi Nhu Quynh, Nguyen Hai Anh, Pham Thi Nhan, Akihiro Matsui, Satoshi Takahashi, Maho Tanaka, Ngo Minh Anh, Nguyen Van Dong, Le Huy Ham, Asuka Higo, Truong Minh Hoa, Manabu Ishitani, Nguyen Ba Nhat Minh, Nguyen Huu Hy, Pao Srean, Vu Anh Thu, Nguyen Ba Tung, Nguyen Anh Vu, Kaho Yamaguchi, Hiroyuki Tsuji, Yoshinori Utsumi, Motoaki Seki

    Plant Molecular Biology   Vol. 109 ( 3 ) page: 233 - 248   2020.9

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    KEY MESSAGE: The field survey in this article showed in 'KU50', a popular variety and late-branching type of cassava in Southeast Asia, that flowering rarely occurs in normal-field conditions in Southeast Asia but is strongly induced in the dry season in the mountainous region. Flowering time is correlated with the expression patterns of MeFT1 and homologs of Arabidopsis GI, PHYA, and NF-Ys. Cassava (Manihot esculenta Crantz) is a tropical crop that is propagated vegetatively rather than sexually by seed. Flowering rarely occurs in the erect-type variety grown in Southeast Asia, but it is known that cassava produces flowers every year in mountainous regions. Data pertaining to the effect of environmental factors on flowering time and gene expression in cassava, however, is limited. The aim of the present study was to determine the kinds of environmental conditions that regulate flowering time in cassava and the underlying molecular mechanisms. The flowering status of KU50, a popular variety in Southeast Asia and late-branching type of cassava, was monitored in six fields in Vietnam and Cambodia. At non-flowering and flowering field locations in North Vietnam, the two FLOWERING LOCUS T (FT)-like genes, MeFT1 and MeFT2, were characterized by qPCR, and the pattern of expression of flowering-related genes and genes responsive to environmental signals were analyzed by using RNA sequencing data from time-series samples. Results indicate that cassava flowering was induced in the dry season in the mountain region, and that flowering time was correlated with the expression of MeFT1, and homologs of Arabidopsis GI, PHYA, and NF-Ys. Based upon these data, we hypothesize that floral induction in cassava is triggered by some conditions present in the mountain regions during the dry season.

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  22. Life-Course Monitoring of Endogenous Phytohormone Levels under Field Conditions Reveals Diversity of Physiological States among Barley Accessions.

    Takashi Hirayama, Daisuke Saisho, Takakazu Matsuura, Satoshi Okada, Kotaro Takahagi, Asaka Kanatani, Jun Ito, Hiroyuki Tsuji, Yoko Ikeda, Keiichi Mochida

    Plant & Cell Physiology   Vol. 61 ( 8 ) page: 1438 - 1448   2020.8

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    Agronomically important traits often develop during the later stages of crop growth as consequences of various plant-environment interactions. Therefore, the temporal physiological states that change and accumulate during the crop's life course can significantly affect the eventual phenotypic differences in agronomic traits among crop varieties. Thus, to improve productivity, it is important to elucidate the associations between temporal physiological responses during the growth of different crop varieties and their agronomic traits. However, data representing the dynamics and diversity of physiological states in plants grown under field conditions are sparse. In this study, we quantified the endogenous levels of five phytohormones - auxin, cytokinins (CKs), ABA, jasmonate and salicylic acid - in the leaves of eight diverse barley (Hordeum vulgare) accessions grown under field conditions sampled weekly over their life course to assess the ongoing fluctuations in hormone levels in the different accessions under field growth conditions. Notably, we observed enormous changes over time in the development-related plant hormones, such as auxin and CKs. Using 3' RNA-seq-based transcriptome data from the same samples, we investigated the expression of barley genes orthologous to known hormone-related genes of Arabidopsis throughout the life course. These data illustrated the dynamics and diversity of the physiological states of these field-grown barley accessions. Together, our findings provide new insights into plant-environment interactions, highlighting that there is cultivar diversity in physiological responses during growth under field conditions.

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  23. WEG1, which encodes a cell wall hydroxyproline-rich glycoprotein, is essential for parental root elongation controlling lateral root formation in rice. Reviewed International journal

    Nonawin Lucob-Agustin, Tsubasa Kawai, Misuzu Takahashi-Nosaka, Mana Kano-Nakata, Cornelius M Wainaina, Tomomi Hasegawa, Mayuko Inari-Ikeda, Moeko Sato, Hiroyuki Tsuji, Akira Yamauchi, Yoshiaki Inukai

    Physiologia Plantarum   Vol. 169 ( 2 ) page: 214 - 227   2020.6

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    Lateral roots (LRs) determine the overall root system architecture, thus enabling plants to efficiently explore their underground environment for water and nutrients. However, the mechanisms regulating LR development are poorly understood in monocotyledonous plants. We characterized a rice mutant, wavy root elongation growth 1 (weg1), that produced higher number of long and thick LRs (L-type LRs) formed from the curvatures of its wavy parental roots caused by asymmetric cell growth in the elongation zone. Consistent with this phenotype, was the expression of the WEG1 gene, which encodes a putative member of the hydroxyproline-rich glycoprotein family that regulates cell wall extensibility, in the root elongation zone. The asymmetric elongation growth in roots is well known to be regulated by auxin, but we found that the distribution of auxin at the apical region of the mutant and the wild-type roots was symmetric suggesting that the wavy root phenotype in rice is independent of auxin. However, the accumulation of auxin at the convex side of the curvatures, the site of L-type LR formation, suggested that auxin likely induced the formation of L-type LRs. This was supported by the need of a high amount of exogenous auxin to induce the formation of L-type LRs. These results suggest that the MNU-induced weg1 mutated gene regulates the auxin-independent parental root elongation that controls the number of likely auxin-induced L-type LRs, thus reflecting its importance in improving rice root architecture.

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  24. Training instance segmentation neural network with synthetic datasets for crop seed phenotyping. International journal

    Yosuke Toda, Fumio Okura, Jun Ito, Satoshi Okada, Toshinori Kinoshita, Hiroyuki Tsuji, Daisuke Saisho

    Communications Biology   Vol. 3 ( 1 ) page: 173 - 173   2020.4

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    In order to train the neural network for plant phenotyping, a sufficient amount of training data must be prepared, which requires time-consuming manual data annotation process that often becomes the limiting step. Here, we show that an instance segmentation neural network aimed to phenotype the barley seed morphology of various cultivars, can be sufficiently trained purely by a synthetically generated dataset. Our attempt is based on the concept of domain randomization, where a large amount of image is generated by randomly orienting the seed object to a virtual canvas. The trained model showed 96% recall and 95% average Precision against the real-world test dataset. We show that our approach is effective also for various crops including rice, lettuce, oat, and wheat. Constructing and utilizing such synthetic data can be a powerful method to alleviate human labor costs for deploying deep learning-based analysis in the agricultural domain.

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  25. High performance of proposed FAB source for surface-activated bonding by plasma analysis

    MORISAKI Ryo, SAKURAI Jumpei, OKA Chiemi, YAMAZAKI Takahiro, HIRAI Takami, TAKAHASHI Tomonori, TSUJI Hiroyuki, OHNO Noriyasu, HATA Seiichi

    The Proceedings of Mechanical Engineering Congress, Japan   Vol. 2020 ( 0 ) page: J13105   2020

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    <p>The novel fast atom beam source (FAB) for surface-activated bonding (SAB) method was proposed in order to improve the lifetime of FAB sources. The proposed FAB source achieved the higher efficiency of Ar-FAB irradiation and less wear on the electrodes. This previously study indicated that the applied magnetic fields made the Ar ions converge on the irradiation port, increasing Ar irradiation efficiency. However, it was not measured and not clearly why the proposed FAB source achieved higher performance. In this study, we aim to reveal the factor of achievement high performance and to propose the new structure of the FAB source with much higher performance by plasma analysis. Electrostatic probe studies and the simulation for Ar plasma in the proposed source show that the electron and Ar<sup>+</sup> density increase near the irradiation port. Moreover, increasing Ar<sup>+</sup> flux only to the wall with irradiation port indicates improving Ar-FAB irradiation efficiency and suppressing Ar+ sputtering and carbon agglomerates on inside wall of the source.</p>

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  26. TILLERS ABSENT1, the WUSCHEL ortholog, is not involved in stem cell maintenance in the shoot apical meristem in rice. Reviewed International journal

    Chie Suzuki, Wakana Tanaka, Hiroyuki Tsuji, Hiro-Yuki Hirano

    Plant Signaling & Behavior   Vol. 14 ( 9 ) page: 1640565 - 1640565   2019.9

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    Stem cell maintenance in the shoot apical meristem (SAM) is very important for plant development and is regulated by the WUSCHEL-CLAVATA (WUS-CLV) feedback loop in Arabidopsis (Arabidopsis thaliana). WUS promotes stem cell identity, whereas CLV negatively regulates stem cell proliferation by repressing WUS expression. We previously showed that, in rice (Oryza sativa), the WUS ortholog TILLERS ABSENT1 (TAB1, also known as OsWUS) has no function in SAM maintenance, whereas it plays a crucial role in axillary meristem development. Recently, we showed that a double mutant of FLORAL ORGAN NUMBER2 (FON2) and ABERRANT SPIKELET AND PANICLE1 (ASP1) led to a marked enlargement of the inflorescence meristem, and that the TAB1 function is not associated with massive stem cells in this meristem. In this paper, we confirmed that TAB1 is also unrelated to the enlargement of the SAM in the vegetative phase of the fon2 and fon2 asp1 mutants. In addition, misexpression of TAB1 under the promoter of FON1 led to a slight reduction of the SAM size in wild type, suggesting that TAB1 is not a positive regulator of stem cells. Taking together, TAB1 seems not to be involved in meristem maintenance, irrespective of the meristem type.

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  27. 表面活性化接合用新規高速原子ビームガン

    森崎 諒, 岡 智絵美, 櫻井 淳平, 平井 隆巳, 高橋 知典, 辻 裕之, 秦 誠一

    精密工学会学術講演会講演論文集   Vol. 2019A ( 0 ) page: 687 - 688   2019.8

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    <p>表面活性化接合は半導体分野における三次元積層技術として利用されている.しかし,表面活性化接合に用いられる高速原子ビームガンは,短時間の使用で内部が磨耗してしまう.そこで,高速原子ビームの高効率な照射により,接合回数に対する相対的な長寿命化を目指す新規高速原子ビームガンの開発を行った.また,新規高速原子ビームガンを用いて,その照射特性を評価し,更なる高性能化を目指した設計指針の確立を目指した.</p>

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  28. Regulation of stomatal opening and histone modification by photoperiod in Arabidopsis thaliana. Reviewed International journal

    Saya Aoki, Shigeo Toh, Norihito Nakamichi, Yuki Hayashi, Yin Wang, Takamasa Suzuki, Hiroyuki Tsuji, Toshinori Kinoshita

    Scientific Reports   Vol. 9 ( 1 ) page: 10054 - 10054   2019.7

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    Stomatal movements are regulated by many environmental signals, such as light, CO2, temperature, humidity, and drought. Recently, we showed that photoperiodic flowering components have positive effects on light-induced stomatal opening in Arabidopsis thaliana. In this study, we determined that light-induced stomatal opening and increased stomatal conductance were larger in plants grown under long-day (LD) conditions than in those grown under short-day (SD) conditions. Gene expression analyses using purified guard cell protoplasts revealed that FT and SOC1 expression levels were significantly increased under LD conditions. Interestingly, the enhancement of light-induced stomatal opening and increased SOC1 expression in guard cells due to LD conditions persisted for at least 1 week after plants were transferred to SD conditions. We then investigated histone modification using chromatin immunoprecipitation-PCR, and observed increased trimethylation of lysine 4 on histone 3 (H3K4) around SOC1. We also found that LD-dependent enhancement of light-induced stomatal opening and H3K4 trimethylation in SOC1 were suppressed in the ft-2 mutant. These results indicate that photoperiod is an important environmental cue regulating stomatal opening, and that LD conditions enhance light-induced stomatal opening and epigenetic modification (H3K4 trimethylation) around SOC1, a positive regulator of stomatal opening, in an FT-dependent manner. Thus, this study provides novel insights into stomatal responses to photoperiod.

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  29. An optimized isolation protocol yields high-quality RNA from cassava tissues (Manihot esculenta Crantz). Reviewed International journal

    Babak Behnam, Adriana Bohorquez-Chaux, Oscar Fernando Castaneda-Mendez, Hiroyuki Tsuji, Manabu Ishitani, Luis Augusto Becerra Lopez-Lavalle

    FEBS Open Bio   Vol. 9 ( 4 ) page: 814 - 825   2019.4

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    We developed and modified a precise, rapid, and reproducible protocol isolating high-quality RNA from tissues of multiple varieties of cassava plants (Manihot esculenta Crantz). The resulting method is suitable for use in mini, midi, and maxi preparations and rapidly achieves high total RNA yields (170-600 μg·g-1) using low-cost chemicals and consumables and with minimal contamination from polysaccharides, polyphenols, proteins, and other secondary metabolites. In particular, A260 : A280 ratios were > 2.0 for RNA from various tissues, and all of the present RNA samples yielded ribosomal integrity number values of greater than six. The resulting high purity and quality of isolated RNA will facilitate downstream applications (quantitative reverse transcriptase-polymerase chain reaction or RNA sequencing) in cassava molecular breeding.

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  30. Development of the VIGS System in the Dioecious Plant Silene latifolia

    Fujita Naoko, Kazama Yusuke, Yamagishi Noriko, Watanabe Kyoko, Ando Saki, Tsuji Hiroyuki, Kawano Shigeyuki, Yoshikawa Nobuyuki, Komatsu Ken

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   Vol. 20 ( 5 )   2019.3

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    (1) Background: Silene latifolia is a dioecious plant, whose sex is determined by XY-type sex chromosomes. Microbotryum lychnidis-dioicae is a smut fungus that infects S. latifolia plants and causes masculinization in female flowers, as if Microbotryum were acting as a sex-determining gene. Recent large-scale sequencing efforts have promised to provide candidate genes that are involved in the sex determination machinery in plants. These candidate genes are to be analyzed for functional characterization. A virus vector can be a tool for functional gene analyses; (2) Methods: To develop a viral vector system in S. latifolia plants, we selected Apple latent spherical virus (ALSV) as an appropriate virus vector that has a wide host range; (3) Results: Following the optimization of the ALSV inoculation method, S. latifolia plants were infected with ALSV at high rates in the upper leaves. In situ hybridization analysis revealed that ALSV can migrate into the flower meristems in S. latifolia plants. Successful VIGS (virus-induced gene silencing) in S. latifolia plants was demonstrated with knockdown of the phytoene desaturase gene. Finally, the developed method was applied to floral organ genes to evaluate its usability in flowers; (4) Conclusion: The developed system enables functional gene analyses in S. latifolia plants, which can unveil gene functions and networks of S. latifolia plants, such as the mechanisms of sex determination and fungal-induced masculinization.

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  31. TFL1-Like Proteins in Rice Antagonize Rice FT-Like Protein in Inflorescence Development by Competition for Complex Formation with 14-3-3 and FD Reviewed

    Miho Kaneko-Suzuki, Rie Kurihara-Ishikawa, Chiaki Okushita-Terakawa, Chojiro Kojima, Misa Nagano-Fujiwara, Izuru Ohki, Hiroyuki Tsuji, Ko Shimamoto, Ken-Ichiro Taoka

    Plant and Cell Physiology   Vol. 59 ( 3 ) page: 458 - 468   2018.3

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    Hd3a, a rice homolog of FLOWERING LOCUS T (FT), is a florigen that induces flowering. Hd3a forms a ternary - florigen activation complex' (FAC) with 14-3-3 protein and OsFD1 transcription factor, a rice homolog of FD that induces transcription of OsMADS15, a rice homolog of APETALA1 (AP1), which leads to flowering. TERMINAL FLOWER 1 (TFL1) represses flowering and controls inflorescence architecture. However, the molecular basis for floral repression by TFL1 remains poorly understood. Here we show that RICE CENTRORADIALIS (RCN), rice TFL1-like proteins, compete with Hd3a for 14-3-3 binding. All four RCN genes are predominantly expressed in the vasculature, and RCN proteins are transported to the shoot apex to antagonize florigen activity and regulate inflorescence development. The antagonistic function of RCN to Hd3a is dependent on its 14-3-3 binding activity. Our results suggest a molecular basis for regulation of the balance between florigen FT and anti-florigen TFL1.

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  32. Development of the Novel Fast Atom Beam Gun

    MORISAKI Ryo, HIRAI Yuki, MIZOSHIRI Mizue, SAKURAI Junpei, HIRAI Takami, TAKAHASHI Tomonori, TSUJI Hiroyuki, HATA Seiichi

    The Proceedings of Mechanical Engineering Congress, Japan   Vol. 2018 ( 0 ) page: J1110203   2018

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    <p>Surface activated bonding (SAB) is used in three-dimensional laminating technology for such as LSI chips. Ar fast atom beam (FAB) is used for SAB. However, there is a serious problem that the conventional FAB gun can be often used for only hundreds of minutes because abrasion powders are generated from the worn part due to Ar<sup>+</sup> sputtering in the gun. Therefore, it is the purpose of this work to develop a new FAB gun to realize longer life. We developed the novel FAB gun applied with a magnetic field to control Ar<sup>+</sup> motion and reduce the sputtering in the gun. Furthermore, the disposition of the carbon electrodes in the gun was changed. As a result, we succeeded in 3 times Ar emission efficiency due to applying the magnetic field. And we unconfirmed abrasion powder in the novel FAB gun after 5200 minutes irradiation experiments. As written above, the novel FAB gun was able to function as expected.</p>

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  33. Imaging florigen distribution <i>in vivo </i>

    Saihara Noriko, Tsuji Hiroyuki

    PLANT MORPHOLOGY   Vol. 29 ( 1 ) page: 27 - 31   2017

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    <p>Florigen is a mobile signal that initiates flowering, which is generated in leaves in response to various environmental stimuli and is transported to the shoot apical meristem (SAM) in plants. The molecular nature of florigen was found to be proteins encoded by the gene <i>FLOWERING LOCUS T</i> (<i>FT</i>) and its orthologs. Recent progress in the molecular biology of florigen revealed its receptors and a transcriptional complex composed of florigen, receptor and transcription factors. <i>In vivo</i> imaging of florigen distribution in the shoot apex and inside a cell contributed to elucidate the essential mechanisms for florigen function. In rice shoot apex, distribution of florigen is clearly visualized by expression of FT protein fused with green fluorescent protein (GFP), and the spatial patterns of downstream gene expression are also visualized by various techniques. At the cellular level, the distribution of florigen and its receptor complex is observed through bimolecular fluorescent complementation (BiFC), which revealed dynamic changes of subcellular localization for florigen and related proteins during the formation of florigen-receptor complex. Here the technique for dissecting SAM is presented to show how SAM samples are prepared for imaging florigen, and recent advances in the regulation of flowering in relation to the contributions from the application of imaging techniques are summarized.</p>

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  34. Molecular function of florigen

    Tsuji Hiroyuki

    Breeding Science   Vol. 67 ( 4 ) page: 327 - 332   2017

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    <p>Florigen is a mobile flowering signal in plants that has a strong impact on plant reproduction and is considered one of the important targets for crop improvement. At the molecular level, florigen is represented as a protein product encoded by the <i>FLOWERING LOCUS T</i> (<i>FT</i>) gene, which is highly conserved across flowering plants and thus the understanding of this protein is expected to be applied to the improvement of many crops. Recent advances in molecular genetics, cell biology and structural biology in plants revealed the presence of intercellular receptors for florigen, a transcriptional complex essential for florigen to function, and also shed light on the molecular basis of pleiotropic function of florigen beyond flowering. Furthermore, cutting-edge technologies, such as live cell imaging and next generation sequencing revealed the precise distribution of florigen and transcriptional targets of the florigen activation complex (FAC) during early stages of floral transition. These understandings will help future crop improvement through the regulation of flowering and other plant developmental processes.</p>

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  35. フロリゲン受容体の発見とその後 Reviewed

    辻寛之, 田岡健一郎

    化学と生物   Vol. 54   page: 358 - 364   2016.4

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  36. Hd3a promotes lateral branching in rice Reviewed

    Hiroyuki Tsuji, Chika Tachibana, Shojiro Tamaki, Ken-ichiro Taoka, Junko Kyozuka, Ko Shimamoto

    Plant Journal   Vol. 82 ( 2 ) page: 256 - 266   2015.4

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    Accumulating evidence indicates that the FLOWERING LOCUST (FT) protein is the mobile floral signal known as florigen. A rice FT homolog, Heading date3a (Hd3a), is transported from the phloem to shoot apical cells, where it interacts with 14-3-3 proteins and transcription factor OsFD1 to form a florigen activation complex (FAC) that activates a rice homolog of the floral identity gene APETALA1. Recent studies showed that florigen has roles in plant development beyond flowering; however, the exact nature of these roles is not well understood. It is not clear whether FT is transported to organs outside the shoot apex, and whether FAC formation is required for processes other than flowering. We show here that the Hd3a protein accumulates in axillary meristems to promote branching, and that FAC formation is required. Analysis of transgenic plants revealed that Hd3a promotes branching through lateral bud outgrowth. Hd3a protein produced in the phloem reached the axillary meristem in the lateral bud, and its transport was required for promotion of branching. Moreover, mutant Hd3a proteins defective in FAC formation but competent with respect to transport did not promote branching. Finally, we show that Hd3a promotes branching independently from strigolactone and FC1, a transcription factor that inhibits branching in rice. Together, these results suggest that Hd3a functions as a mobile signal for branching in rice.
    Significance Statement Accumulating evidence indicates that FLOWERING LOCUS T (FT) protein is the mobile floral signal florigen. Recent studies highlight that florigen may play multiple roles beyond flowering, whereas importance of mobile nature and molecular mechanism underling pleiotropic function remain unknown. We show here that phloem-expressed Hd3a protein accumulated in the distant axillary meristems to promote branching and that FAC formation was required, suggesting that Hd3a functions as a mobile signal for branching in rice.

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  37. FT-like proteins induce transposon silencing in the shoot apex during floral induction in rice. Reviewed

    Tamaki, S., Tsuji, H (Corresponding Author)., Matsumoto, A., Fujita, A., Shimatani, Z., Terada, R., Sakamoto, T., Kurata, T., Shimamoto, K.

    Proc. Natl. Acad. Sci. USA   Vol. 112   page: 901 - 911   2015.1

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  38. Dual role of tree florigen activation complex component FD in photoperiodic growth control and adaptive response pathways. Reviewed

    *Tylewicz, S., *Tsuji, H., *Miskoloczi, P. , Petterle, A., Azeez, A., Jonsson, K., Shimamoto, K., Bhalerao, R.P.

    Proc. Natl. Acad. Sci. USA   Vol. 112   page: 3140 - 3145   2015.1

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  39. Heterotrimeric G proteins control stem cell proliferation through CLAVATA signaling in Arabidopsis Reviewed

    Takashi Ishida, Ryo Tabata, Masashi Yamada, Mitsuhiro Aida, Kanako Mitsumasu, Masayuki Fujiwara, Katsushi Yamaguchi, Shuji Shigenobu, Masayuki Higuchi, Hiroyuki Tsuji, Ko Shimamoto, Mitsuyasu Hasebe, Hiroo Fukuda, Shinichiro Sawa

    EMBO REPORTS   Vol. 15 ( 11 ) page: 1202 - 1209   2014.11

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    Cell-to-cell communication is a fundamental mechanism for coordinating developmental and physiological events in multicellular organisms. Heterotrimeric G proteins are key molecules that transmit extracellular signals; similarly, CLAVATA signaling is a crucial regulator in plant development. Here, we show that Arabidopsis thaliana G mutants exhibit an enlarged stem cell region, which is similar to that of clavata mutants. Our genetic and cell biological analyses suggest that the G protein beta-subunit1 AGB1 and RPK2, one of the major CLV3 peptide hormone receptors, work synergistically in stem cell homeostasis through their physical interactions. We propose that AGB1 and RPK2 compose a signaling module to facilitate meristem development.

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  40. Split luciferase complementation assay to detect regulated protein-protein interactions in rice protoplasts in a large-scale format Reviewed

    Yukichi Fujikawa, Takahiro Nakanishi, Hiroko Kawakami, Kanako Yamasaki, Masa H. Sato, Hiroyuki Tsuji, Makoto Matsuoka, Naohiro Kato

    RICE   Vol. 7 ( 1 ) page: 11   2014.6

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    Background: The rice interactome, in which a network of protein-protein interactions has been elucidated in rice, is a useful resource to identify functional modules of rice signal transduction pathways. Protein-protein interactions occur in cells in two ways, constitutive and regulative. While a yeast-based high-throughput method has been widely used to identify the constitutive interactions, a method to detect the regulated interactions is rarely developed for a large-scale analysis.
    Results: A split luciferase complementation assay was applied to detect the regulated interactions in rice. A transformation method of rice protoplasts in a 96-well plate was first established for a large-scale analysis. In addition, an antibody that specifically recognizes a carboxyl-terminal fragment of Renilla luciferase was newly developed. A pair of antibodies that recognize amino-and carboxyl-terminal fragments of Renilla luciferase, respectively, was then used to monitor quality and quantity of interacting recombinant-proteins accumulated in the cells. For a proof-of-concept, the method was applied to detect the gibberellin-dependent interaction between GIBBERELLIN INSENSITIVE DWARF1 and SLENDER RICE 1.
    Conclusions: A method to detect regulated protein-protein interactions was developed towards establishment of the rice interactome.

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  41. Florigen signaling Reviewed

    Hiroyuki Tsuji, Ken-ichiro Taoka

    Enzymes   Vol. 35   page: 113 - 144   2014

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    Florigen is a systemic signal that promotes flowering. Its molecular nature is a conserved FLOWERING LOCUS T (FT) protein that belongs to the PEBP family. FT is expressed in the leaf phloem and transported to the shoot apical meristem where it initiates floral transition. In the cells of the meristem, FT binds 14-3-3 proteins and bZIP transcription factor FD to form the florigen activation complex, FAC, which activates floral meristem identity genes such as AP1. The FAC model provides molecular basis for multiple functions of FT beyond flowering through changes of its partners and transcriptional targets. The surface of FT protein includes several regions essential for transport and functions, suggesting the binding of additional components that support its function. FT expression is under photoperiodic control, involving a conserved GIGANTEA-CONSTANS-FT regulatory module with species-specific modifications that contribute variations of flowering time in natural populations.

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  42. Florigen in rice: complex gene network for florigen transcription, florigen activation complex, and multiple functions Reviewed

    Hiroyuki Tsuji, Ken-ichiro Taoka, Ko Shimamoto

    CURRENT OPINION IN PLANT BIOLOGY   Vol. 16 ( 2 ) page: 228 - 235   2013.5

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    Regulation of flowering time directly influences successful rice grain production; thus, the long history of domestication and breeding has improved the genetic network of flowering. Recent advances using molecular genomic approaches have revealed the targets of these modifications and the underlying molecular mechanism for flowering. These efforts contributed to identifying the molecular nature of the systemic floral signal `florigen' and have shown how florigen functions, how florigen expression is controlled, and how regulatory pathways are diversified. In this review, we summarize the advances in our understanding of the detailed molecular and genetic mechanisms that allow rice plants to produce flowers at the proper time to ensure grain production.

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  43. Structure and function of florigen and the receptor complex Reviewed

    Ken-ichiro Taoka, Izuru Ohki, Hiroyuki Tsuji, Chojiro Kojima, Ko Shimamoto

    TRENDS IN PLANT SCIENCE   Vol. 18 ( 5 ) page: 287 - 294   2013.5

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    In the 1930s, the flowering hormone, florigen, was proposed to be synthesized in leaves under inductive day length and transported to the shoot apex, where it induces flowering. More recently, generated genetic and biochemical data suggest that florigen is a protein encoded by the gene, FLOWERING LOCUS T (FT). A rice (Oryza sativa) FT homolog, Hd3a, interacts with the rice FD homolog, OsFD1, via a 14-3-3 protein. Formation of this tri-protein complex is essential for flowering promotion by Hd3a in rice. In addition, the multifunctionality of FT homologs, other than for flowering promotion, is an emerging concept. Here we review the structural and biochemical features of the florigen protein complex and discuss the molecular basis for the multifunctionality of FT proteins.

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  44. Functional Diversification of FD Transcription Factors in Rice, Components of Florigen Activation Complexes Reviewed

    Hiroyuki Tsuji, Hiroyuki Nakamura, Ken-ichiro Taoka, Ko Shimamoto

    PLANT AND CELL PHYSIOLOGY   Vol. 54 ( 3 ) page: 385 - 397   2013.3

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    Florigen, a protein encoded by the FLOWERING LOCUS T (FT) in Arabidopsis and Heading date 3a (Hd3a) in rice, is the universal flowering hormone in plants. Florigen is transported from leaves to the shoot apical meristem and initiates floral evocation. In shoot apical cells, conserved cytoplasmic 14-3-3 proteins act as florigen receptors. A hexameric florigen activation complex (FAC) composed of Hd3a, 14-3-3 proteins, and OsFD1, a transcription factor, activates OsMADS15, a rice homolog of Arabidopsis APETALA1, leading to flowering. Because FD is a key component of the FAC, we characterized the FD gene family and their functions. Phylogenetic analysis of FD genes indicated that this family is divided into two groups: (i) canonical FD genes that are conserved among eudicots and non-Poaceae monocots; and (ii) Poaceae-specific FD genes that are organized into three subgroups: Poaceae FD1, FD2 and FD3. The Poaceae FD1 group shares a small sequence motif, T(A/V)LSLNS, with FDs of eudicots and non-Poaceae monocots. Overexpression of OsFD2, a member of the Poaceae FD2 group, produced smaller leaves with shorter plastochrons, suggesting that OsFD2 controls leaf development. In vivo subcellular localization of Hd3a, 14-3-3 and OsFD2 suggested that in contrast to OsFD1, OsFD2 is restricted to the cytoplasm through its interaction with the cytoplasmic 14-3-3 proteins, and interaction of Hd3a with 14-3-3 facilitates nuclear translocation of the FAC containing OsFD2. These results suggest that FD function has diverged between OsFD1 and OsFD2, but formation of a FAC is essential for their function.

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  45. Flowering Reviewed

    Hiroyuki Tsuji, Ko Shimamoto

    Genetics and Genomics of Rice     page: 269 - 278   2013.1

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  46. Regulation of flowering in rice: two florigen genes, a complex gene network, and natural variation Reviewed

    Hiroyuki Tsuji, Ken-ichiro Taoka, Ko Shimamoto

    CURRENT OPINION IN PLANT BIOLOGY   Vol. 14 ( 1 ) page: 45 - 52   2011.2

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    Photoperiodic control of flowering time consists of a complicated network that converges into the generation of a mobile flowering signal called florigen. Recent advances identifying the protein FT/Hd3a as the molecular nature responsible for florigen activity have focused current research on florigen genes as the important output of this complex signaling network. Rice is a model system for short-day plants and recent progress in elucidating the flowering network from rice and Arabidopsis, a long-day plant, provides an evolutionarily comparative view of the photoperiodic flowering pathway. This review summarizes photoperiodic flowering control in rice, including the interaction of complex layers of gene networks contributed from evolutionarily unique factors and the regulatory adaptation of conserved factors.

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  47. Characterization of the Molecular Mechanism Underlying Gibberellin Perception Complex Formation in Rice Reviewed

    Ko Hirano, Kenji Asano, Hiroyuki Tsuji, Mayuko Kawamura, Hitoshi Mori, Hidemi Kitano, Miyako Ueguchi-Tanaka, Makoto Matsuoka

    PLANT CELL   Vol. 22 ( 8 ) page: 2680 - 2696   2010.8

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    The DELLA protein SLENDER RICE1 (SLR1) is a repressor of gibberellin (GA) signaling in rice (Oryza sativa), and most of the GA-associated responses are induced upon SLR1 degradation. It is assumed that interaction between GIBBERELLIN INSENSITIVE DWARF1 (GID1) and the N-terminal DELLA/TVHYNP motif of SLR1 triggers F-box protein GID2-mediated SLR1 degradation. We identified a semidominant dwarf mutant, Slr1-d4, which contains a mutation in the region encoding the C-terminal GRAS domain of SLR1 (SLR1(G576V)). The GA-dependent degradation of SLR1(G576V) was reduced in Slr1-d4, and compared with SLR1, SLR1(G576V) showed reduced interaction with GID1 and almost none with GID2 when tested in yeast cells. Surface plasmon resonance of GID1-SLR1 and GID1-SLR1(G576V) interactions revealed that the GRAS domain of SLR1 functions to stabilize the GID1-SLR1 interaction by reducing its dissociation rate and that the G576V substitution in SLR1 diminishes this stability. These results suggest that the stable interaction of GID1-SLR1 through the GRAS domain is essential for the recognition of SLR1 by GID2. We propose that when the DELLA/TVHYNP motif of SLR1 binds with GID1, it enables the GRAS domain of SLR1 to interact with GID1 and that the stable GID1-SLR1 complex is efficiently recognized by GID2.

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  48. The 14-3-3 Protein GF14c Acts as a Negative Regulator of Flowering in Rice by Interacting with the Florigen Hd3a Reviewed

    Yekti Asih Purwestri, Yuka Ogaki, Shojiro Tamaki, Hiroyuki Tsuji, Ko Shimamoto

    PLANT AND CELL PHYSIOLOGY   Vol. 50 ( 3 ) page: 429 - 438   2009.3

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    Hd3a and FT proteins have recently been proposed to act as florigens in rice and Arabidopsis, respectively; however, the molecular mechanisms of their function remain to be determined. In this study, we identified GF14c (a 14-3-3 protein) as an Hd3a-interacting protein in a yeast two-hybrid screen. In vitro and in vivo experiments, using a combination of pull-down assays and bimolecular fluorescence complementation, confirmed the interaction between Hd3a and GF14c. Functional analysis using either GF14c overexpression or knockout transgenic rice plants indicated that this interaction plays a role in the regulation of flowering. GF14c-overexpressing plants exhibited a delay in flowering and the knockout mutants displayed early flowering relative to the wild-type plants under short-day conditions. These results suggest that GF14c acts as a negative regulator of flowering by interacting with Hd3a. Since the 14-3-3 protein has been shown to interact with FT protein in tomato and Arabidopsis, our results in rice provide important findings about FT signaling in plants.

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  49. Florigen and the Photoperiodic Control of Flowering in Rice Reviewed

    Hiroyuki Tsuji, Shojiro Tamaki, Reina Komiya, Ko Shimamoto

    RICE   Vol. 1 ( 1 ) page: 25 - 35   2008.9

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    Flowering time is a key trait for geographical and seasonal adaptation of plants and is an important consideration for rice breeders. Recently identified genetic factors provide new insights into this complex trait. The list of genes involved in flowering and their functions tells us that the molecular basis of day-length measurement includes both of the evolution of unique factors and the regulatory adaptation of conserved factors in rice. This information helped identify rice florigen, a mobile flowering signal. Our current view of flowering time regulation incorporates the presence of complex layers of gene networks integrated with the synthesis of florigen protein and its subsequent transport and perception.

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  50. GAMYB controls different sets of genes and is differentially regulated by microRNA in aleurone cells and anthers. Reviewed International journal

    Hiroyuki Tsuji, Koichiro Aya, Miyako Ueguchi-Tanaka, Yukihisa Shimada, Mikio Nakazono, Ryosuke Watanabe, Naoko K Nishizawa, Kenji Gomi, Asako Shimada, Hidemi Kitano, Motoyuki Ashikari, Makoto Matsuoka

    The Plant journal : for cell and molecular biology   Vol. 47 ( 3 ) page: 427 - 444   2006.8

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    GAMYB is a component of gibberellin (GA) signaling in cereal aleurone cells, and has an important role in flower development. However, it is unclear how GAMYB function is regulated. We examined the involvement of a microRNA, miR159, in the regulation of GAMYB expression in cereal aleurone cells and flower development. In aleurone cells, no miR159 expression was observed with or without GA treatment, suggesting that miR159 is not involved in the regulation of GAMYB and GAMYB-like genes in this tissue. miR159 was expressed in tissues other than aleurone, and miR159 over-expressors showed similar but more severe phenotypes than the gamyb mutant. GAMYB and GAMYB-like genes are co-expressed with miR159 in anthers, and the mRNA levels for GAMYB and GAMYB-like genes are negatively correlated with miR159 levels during anther development. Thus, OsGAMYB and OsGAMYB-like genes are regulated by miR159 in flowers. A microarray analysis revealed that OsGAMYB and its upstream regulator SLR1 are involved in the regulation of almost all GA-mediated gene expression in rice aleurone cells. Moreover, different sets of genes are regulated by GAMYB in aleurone cells and anthers. GAMYB binds directly to promoter regions of its target genes in anthers as well as aleurone cells. Based on these observations, we suggest that the regulation of GAMYB expression and GAMYB function are different in aleurone cells and flowers in rice.

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  51. Dynamic and reversible changes in histone H3-Lys4 methylation and H3 acetylation occurring at submergence-inducible genes in rice. Reviewed

    Hiroyuki Tsuji, Hiroaki Saika, Nobuhiro Tsutsumi, Atsushi Hirai, Mikio Nakazono

    Plant & cell physiology   Vol. 47 ( 7 ) page: 995 - 1003   2006.7

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    Histone modifications such as methylation and acetylation in the chromatin surrounding a gene are thought to regulate transcriptional activity. In this study, to determine whether dynamic changes occur in histone modification on the loci of stress-responsive genes in plants, we chose rice submergence-inducible ADH1 and PDC1 genes. When submerged, the rice ADH1 and PDC1 genes were activated in a biphasic manner: the first and second inductions occurred after approximately 2 and 12 h of submergence, respectively. Their expression was transcriptionally induced as shown by increased binding of RNA polymerase II to the ADH1 and PDC1 loci during submergence. The Lys4 residues of the histone H3 proteins (H3-K4s) at both the 5'- and 3'-coding regions of ADH1 and PDC1 were found to change from a di-methylated state to a tri-methylated state at the first induction period. On the other hand, acetylation of H3 increased throughout ADH1 and PDC1 genes at the later induction period. The methylation and acetylation levels recovered to the initial levels during re-aeration. Treatment of seedlings with a histone deacetylase (HDAC) inhibitor, trichostatin A, increased acetylation of histones H3 and association of RNA polymerase II on the ADH1 and PDC1 loci, thereby increasing transcript levels of ADH1 and PDC1. Together, these results showed dynamic and reversible changes of histone H3-K4 methylation and H3 acetylation in stress-responsive genes in a higher plant in response to the appearance or disappearance of an environmental stress.

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  52. Interaction among SLRI1, GID1, and GID2 in the gibberellin signaling pathway in rice cells.

    Tsuji H, Ueguchi-Tanaka M, Nakajima M, Ashikari M, Kitano H, Yamaguchi I, Matsuoka M

    PLANT AND CELL PHYSIOLOGY   Vol. 47   page: S125 - S125   2006

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  53. Microarray analysis of anther-specific genes under the control of GAMYB in rice

    Aya K, Ueguchi-Tanaka M, Kondo M, Tsuji H, Ashikari M, Nishimura M, Matsuoka M

    PLANT AND CELL PHYSIOLOGY   Vol. 47   page: S243 - S243   2006

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  54. Involvement of aldehyde dehydrogenase in alleviation of post-anoxic injury in rice Reviewed

    Naoki Meguro, Hiroyuki Tsuji, Nobuhiro Tsutsumi, Mikio Nakazono, Atsushi Hirai

    Abiotic Stress Tolerance in Plants     page: 111 - 119   2006

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    Oxygen deprivation induced by submergence, flooding and waterlogging is an environmental stress that affects the growth of plants and production of crops. Plants undergo metabolic and morphological changes to avoid or alleviate the stresses arising from low oxygen conditions. One well-known metabolic change is activation of the glycolytic and fermentation pathways, which are important for ATP production under anaerobic conditions. In some plant species, morphological changes include elongation of internodes or petioles, aerenchyma formation and formation of a barrier to radial oxygen loss. Under post-anoxic conditions, plants suffer from injurious substances, reactive oxygen species and acetaldehyde. Plants have various mechanisms for metabolizing these harmful molecules to prevent or alleviate post-anoxic injuries. This paper reviews the current understanding of adaptation and tolerance mechanisms of plants that are activated under low oxygen conditions and following re-aeration. © 2006 Springer. Printed in the Netherlands. © 2006 Springer. All Rights Reserved.

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  55. Interaction among SLR1, GID1, and GID2 in the Gibberellin Signaling Pathway in Rice Cells.

    Tsuji Hiroyuki, Ueguchi-Tanaka Miyako, Nakajima Masatoshi, Ashikari Motoyuki, Kitano Hidemi, Yamaguchi Isomaro, Matsuoka Makoto

    Plant and Cell Physiology Supplement   Vol. 2006 ( 0 ) page: 404 - 404   2006

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    DELLA proteins, which are a subgroup of GRAS proteins, are key factors of gibberellin (GA) signaling. Our previous studies revealed that, in rice cells, DELLA protein SLR1 functions as a repressor for GA action and is degraded GA-dependently by ubiquitin-proteasome system through the SCF<SUP>GID2</SUP> function. However, it is unclear how the GA signal is transduced to SLR1 from bioactive GA molecular. Recently, we succeeded to identify a GA receptor, which has a similar primary structure to that of hormone sensitive lipase and is localized in nucleus. Interestingly, the yeast two-hybrid assay revealed that the interaction between GID1 and SLR1 occurs as a GA dependent manner. Thus we speculate that the complex composed by GID1, SLR1, and GA may recruit an F-box protein, GID2, to initiate the degradation of SLR1. To evaluate this, we have performed some biochemical experiments such as yeast three hybrid assay and pull-down assay in vitro.

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  56. Function and regulation of transcription factor GAMYB and GAMYB-like

    Tsuji H, Ueguchi-Tanaka M, Ashikari M, Kitano H, Matsuoka M

    PLANT AND CELL PHYSIOLOGY   Vol. 46   page: S32 - S32   2005

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  57. Microarray analysis of anther-specific genes under the control of GAMYB in rice

    Aya K, Ueguchi-Tanaka M, Tsuji H, Ashikari M, Matsuoka M

    PLANT AND CELL PHYSIOLOGY   Vol. 46   page: S211 - S211   2005

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  58. Function and Regulation of transcription factor GAMYB and GAMYB-like

    Tsuji Hiroyuki, Ueguchi-Tanaka Miyako, Ashikari Motoyuki, Kitano Hidemi, Matsuoka Makoto

    Plant and Cell Physiology Supplement   Vol. 2005 ( 0 ) page: 031 - 031   2005

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    GAMYB was first isolated as a positive transcriptional regulator of gibberellin (GA)-dependent α-amylase expression in barley endosperm, and its molecular properties has been extensively characterized. Here, we report the role and the regulation of GAMYB and structurally related GAMYB-like proteins in rice growth. We found that <I>GAMYB</I> and <I>GAMYB-like</I> genes contain a target site for a microRNA, miR159, and overexpression of miR159 in rice plant revealed not only the regulatory mode of miR159 on these genes but also the role of these genes in rice growth. On the other hand, microarray experiment revealed that almost all of GA-regulated genes did not respond to GA in the endosperm of <I>gamyb</I>. This suggests that GAMYB is involved in the regulation of almost all GA-mediated gene expression in rice endosperm. <br>This work was in part supported by PROBRAIN.

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  59. Induction of mitochondrial aldehyde dehydrogenase by submergence facilitates oxidation of acetaldehyde during re-aeration in rice. Reviewed International journal

    Hiroyuki Tsuji, Naoki Meguro, Yasuhiro Suzuki, Nobuhiro Tsutsumi, Atsushi Hirai, Mikio Nakazono

    FEBS letters   Vol. 546 ( 2-3 ) page: 369 - 73   2003.7

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    Post-hypoxic injuries in plants are primarily caused by bursts of reactive oxygen species and acetaldehyde. In agreement with previous studies, we found accumulations of acetaldehyde in rice during re-aeration following submergence. During re-aeration, acetaldehyde-oxidizing aldehyde dehydrogenase (ALDH) activity increased, thereby causing the acetaldehyde content to decrease in rice. Interestingly, re-aerated rice plants showed an intense mitochondrial ALDH2a protein induction, even though ALDH2a mRNA was submergence induced and declined upon re-aeration. This suggests that rice ALDH2a mRNA is accumulated in order to quickly metabolize acetaldehyde that is produced upon re-aeration.

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  60. Organ-specific expressions and chromosomal locations of two mitochondrial aldehyde dehydrogenase genes from rice (Oryza sativa L.), ALDH2a and ALDH2b. Reviewed International journal

    Hiroyuki Tsuji, Nobuhiro Tsutsumi, Takuji Sasaki, Atsushi Hirai, Mikio Nakazono

    Gene   Vol. 305 ( 2 ) page: 195 - 204   2003.2

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    Recent studies have suggested that mitochondrial aldehyde dehydrogenase (aldehyde:NAD(P)(+) oxidoreductase, EC 1.2.1.3) (ALDH2) plays essential roles in pollen development in plants. Rice (Oryza sativa L.) ALDH2 is encoded by at least two ALDH2 genes, one of which (ALDH2a) was previously identified. In this study, to understand the roles of ALDH2 in rice, we isolated and characterized a cDNA clone encoding another rice ALDH2 (ALDH2b). An in vitro ALDH assay indicated that ALDH2b possesses an NAD(+)-linked activity for oxidation of acetaldehyde, glycolaldehyde and propionaldehyde. Northern blot and immunoblot analyses revealed that ALDH2b was constitutively present in all the organs examined, whereas ALDH2a was expressed in leaves of dark-grown seedlings and panicles. By RFLP linkage mapping, the ALDH2a and ALDH2b genes were mapped to the long arm of chromosome 2 and the short arm of chromosome 6, respectively. We suggest that the rice ALDH2a and ALDH2b genes are orthologues of maize mitochondrial ALDH genes, rf2b and rf2a, respectively.

    DOI: 10.1016/S0378-1119(03)00383-4

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  61. Organ-Specific Expressions and Chromosomal Locations of Two Mitochondrial Aldehyde Dehydrogenase Genes from Rice (Oryza sativa L), ALDH2a and ALDH2b Reviewed

    Hiroyuki Tsuji, Nobuhiro Tsutsumi, Takuji Sasaki, Atsushi Hirai, Mikio Nakazono

    Gene   Vol. 305 ( 2 ) page: 195 - 204   2003.2

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    DOI: 10.1016/S0378-1119(03)00383-4,

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MISC 104

  1. Data driven crop design technology

    平山隆志, 最相大輔, 井藤純, 服部公央亮, 岡田聡史, 池田陽子, 梅崎太造, 辻寛之, 持田恵一

    日本植物生理学会年会(Web)   Vol. 63rd   2022

  2. Developmental state transition in the shoot apical meristem of barley

    井藤純, 野村有子, 高萩航太郎, 岡田聡史, 久下修平, 佐藤奈緒, 新井駿一, 松本大輝, 杉村みどり, 関緑, 服部公央亮, 梅崎太造, 池田陽子, 最相大輔, 持田恵一, 平山隆志, 辻寛之

    日本植物生理学会年会(Web)   Vol. 63rd   2022

  3. イネ花成ホルモン蛋白質の液-液相分離

    榎本麻由, 安澤すあい, 小泉優香, 田岡健一郎, 児玉高志, 白木賢太郎, 藤原敏道, 辻寛之, 児嶋長次郎, 児嶋長次郎

    日本蛋白質科学会年会プログラム・要旨集   Vol. 21st   2021

  4. Developmental trajectory analysis of the shoot apex in barley by single meristem transcriptome

    井藤純, 野村有子, 佐藤奈緒, 岡田聡史, 高萩航太郎, 杉村みどり, 関緑, 最相大輔, 持田恵一, 平山隆志, 辻寛之

    育種学研究   Vol. 23   2021

  5. Analysis of cellular dynamics in the barley inflorescence meristem during its abortion at the single-cell resolution

    松本大輝, 井藤純, 新井駿一, 野村有子, 杉村みどり, 佐藤奈緒, 関緑, 宇野何岸, 宇野何岸, 佐藤良勝, 佐藤良勝, 最相大輔, 辻寛之

    育種学研究   Vol. 23   2021

  6. Analysis of rice shoot apical meristem and leaves growth trajectory

    吉田綾, 保坂碧, 吉田明希子, 吉田明希子, 井藤純, 辻寛之

    育種学研究   Vol. 23   2021

  7. Genomic research across the barley life cycle toward ’preemptive breeding’

    最相大輔, 岡田聡史, 金谷麻加, 金谷麻加, 池田陽子, 井藤純, 辻寛之, 持田恵一, 持田恵一, 持田恵一, 平山隆志

    育種学研究   Vol. 23   2021

  8. Analysis of cellular dynamics in the shoot apical meristem of barley under field and laboratory conditions by 3D imaging at single-cell resolution

    新井駿一, 井藤純, 久下修平, 佐藤奈緒, 野村有子, 杉村みどり, 最相大輔, 辻寛之

    日本植物生理学会年会(Web)   Vol. 62nd   2021

  9. Data driven crop design technology

    平山隆志, 岡田聡史, 最相大輔, 井藤純, 服部公央亮, 池田陽子, 梅崎太造, 辻寛之, 持田恵一

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 44th   2021

  10. Developmental trajectory analysis of barley life cycle using field transcriptome data

    岡田聡史, 最相大輔, 金谷麻加, 金谷麻加, 池田陽子, 井藤純, 辻寛之, 井上小槙, 上原由紀子, 清水みなみ, 持田恵一, 持田恵一, 持田恵一, 平山隆志

    育種学研究   Vol. 23   2021

  11. Pseudo-time transition of barley life cycle in the field

    最相大輔, 岡田聡史, 金谷麻加, 金谷麻加, 池田陽子, 井藤純, 辻寛之, 持田恵一, 持田恵一, 持田恵一, 平山隆志

    育種学研究   Vol. 23   2021

  12. Analysis of flowering-related genes expression in all leaves and all leaf stage of rice

    吉田綾, 保坂碧, 吉田明希子, 吉田明希子, 辻寛之

    育種学研究   Vol. 22   2020

  13. Genetic analysis on heading time of barley (Hordeum vulgare) under multiple environments

    岡田聡史, 最相大輔, 井藤純, 辻寛之, 高萩航太郎, 持田恵一, 持田恵一, 平山隆志

    育種学研究   Vol. 22   2020

  14. de novo genome assembly of a Japanese wheat cultivar Norin 61 in the international wheat 10+ genomes project

    清水健太郎, 清水健太郎, 半田裕一, 半田裕一, 那須田周平, 瀬々潤, 瀬々潤, 川浦香奈子, 辻寛之, 爲重才覚, 坂智広, DARIO Copetti, 畠山剛臣, 畠山剛臣, 清水(稲継)理恵, CATHARINE Aquino, 小林史典, GUTIERREZ-GONZALEZ Juan, STEIN Nils, DELOREAN Emily, PAAPE Tim, HALSTEAD-NUSSLOCH Gwyneth, HABERER Georg, SPANNAGL Manuel, MAYER Klaus, MASCHER Martin, HIMMELBACH Axel, PADMARASU Sudharsan, WICKER Thomas, POZNIAK Curtis J.

    育種学研究   Vol. 22   2020

  15. Intraspecific variation of field growth dynamics in barley

    最相大輔, 岡田聡史, 井藤純, 辻寛之, 高萩航太郎, 高萩航太郎, 持田恵一, 持田恵一, 持田恵一, 平山隆志

    育種学研究   Vol. 22   2020

  16. Epigenetic regulation of shoot apical meristem methylome

    肥後あすか, 才原徳子, 東陽子, 三浦史仁, 伊藤隆司, 辻寛之

    育種学研究   Vol. 22   2020

  17. Analysis of cellular dynamics in the shoot apical meristem of barley under field conditions at the single-cell resolution

    新井駿一, 久下修平, 佐藤奈緒, 野村有子, 杉村みどり, 最相大輔, 井藤純, 辻寛之

    育種学研究   Vol. 22   2020

  18. イネ茎頂分裂組織の相転換におけるDNAメチル化パターンの動態とその制御機構

    肥後あすか, 三浦史仁, 伊藤隆司, 島本功, 辻寛之

    日本植物生理学会年会(Web)   Vol. 60th   2019

  19. 任意の圃場環境に最適な作物の育種を可能に-データ科学に基づく作物設計基盤技術の開発

    平山隆志, 最相大輔, 井藤純, 高萩航太郎, 香西雄介, 鷲見典克, 池田陽子, 井上小槙, 上原由紀子, 清水みなみ, 服部公央亮, 梅崎太造, 梅崎太造, 辻寛之, 持田恵一, 持田恵一, 持田恵一

    日本生物工学会大会講演要旨集   Vol. 71st   2019

  20. 圃場オオムギを用いた時系列クロマチン修飾解析

    池田陽子, 金谷麻加, 井上小槙, 最相大輔, 井藤純, 辻寛之, 持田恵一, 持田恵一, 持田恵一, 平山隆志

    日本植物生理学会年会(Web)   Vol. 60th   2019

  21. 野外環境におけるオオムギ茎頂メリステムの成長過程の系統間比較

    井藤純, 野村有子, 最相大輔, 高萩航太郎, 高萩航太郎, 持田恵一, 持田恵一, 平山隆志, 辻寛之

    育種学研究   Vol. 21   2019

  22. 花成ホルモン蛋白質のNMR解析および<sup>19</sup>F-NMRスクリーニング

    安澤すあい, 新家粧子, 古板恭子, 小篭蒼, 田岡健一郎, 辻寛之, 藤原敏道, 児嶋長次郎, 児嶋長次郎

    Abstracts. Annual Meeting of the NMR Society of Japan   Vol. 58th (CD-ROM)   2019

  23. 第何葉のフロリゲンが花成を起こすのか?

    吉田綾, 吉田明希子, 辻寛之

    育種学研究   Vol. 21   2019

  24. 多様な花成ホルモン蛋白質の構造・機能解析

    安澤すあい, 新家粧子, 古板恭子, 小篭蒼, 田岡健一郎, 辻寛之, 藤原敏道, 児嶋長次郎

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 42nd   2019

  25. 葉身伸長に基づくオオムギ生長過程の表現型可塑性

    最相大輔, 井藤純, 辻寛之, 平山隆志

    育種学研究   Vol. 20   page: 241   2018.3

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  26. イネ茎頂分裂組織のDNAメチル化パターンの動態と制御機構の解析

    肥後あすか, 才原徳子, 三浦史仁, 東陽子, 山田恵美, 玉置祥二郎, 伊藤佑, 樽谷芳明, 坂本智昭, 藤原正幸, 倉田哲也, 深尾陽一朗, 森藤暁, 寺田理枝, 伊藤隆司, 角谷徹仁, 角谷徹仁, 角谷徹仁, 島本功, 辻寛之

    日本植物生理学会年会(Web)   Vol. 59th   2018

  27. フロリゲン受容体のNMR解析

    中村歩美, 樋口佳恵, 新家粧子, 田岡健一郎, 辻寛之, 藤原敏道, 木川隆則, 児嶋長次郎, 児嶋長次郎

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 41st   2018

  28. フィールド環境下のオオムギの花芽形成過程における茎頂メリステムの応答性の系統間差

    井藤純, 野村有子, 最相大輔, 平山隆志, 辻寛之

    日本植物生理学会年会(Web)   Vol. 59th   page: ROMBUNNO.1aJ02   2018

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  29. ジャガイモの14-3-3タンパク質St14fの結晶構造解析

    原田健一, 新家粧子, 山下栄樹, 田岡健一郎, 辻寛之, 藤原敏道, 中川敦史, 児嶋長次郎, 児嶋長次郎

    日本蛋白質科学会年会プログラム・要旨集   Vol. 18th   2018

  30. 圃場環境下における野生オオムギと栽培オオムギのメリステムの成長過程

    井藤純, 最相大輔, 辻寛之

    育種学研究   Vol. 19   page: 42   2017.10

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  31. イネ幹細胞のDNAメチル化パターンの解析

    肥後あすか, 才原徳子, 三浦史仁, 東陽子, 山田恵美, 玉置祥二郎, 坂本智昭, 藤原正幸, 倉田哲也, 深尾陽一朗, 伊藤隆司, 島本功, 辻寛之

    日本生化学会大会(Web)   Vol. 90th   2017

  32. コムギのフロリゲン遺伝子全同定と合成コムギにおける発現解析

    三橋明奈, 宅見薫雄, 辻寛之, 清水健太郎, 清水健太郎, 辻本壽, 坂智広

    育種学研究   Vol. 18   page: 206   2016.9

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  33. イネの茎頂メリステムにおけるオーキシン情報伝達のシグナリング

    IMAI YASUMICHI, SATO YOSHIKATSU, HIGASHIYAMA TETSUYA, SHIMAMOTO KO, TSUJI HIROYUKI

    育種学研究   Vol. 17   page: 222   2015.3

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  34. イネ茎頂メリステムにおける統合的プロテオミクス

    YAMADA MEGUMI, FUJIHARA MASAYUKI, NOMURA YUKO, NAKAGAMI HIROFUMI, HANADA KOSUKE, FUKAO YOICHIRO, SHIMAMOTO KO, TSUJI HIROYUKI

    育種学研究   Vol. 17   page: 223   2015.3

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  35. 花成ホルモン・フロリゲンの機能に関する遺伝育種学的研究

    TSUJI HIROYUKI

    育種学研究   Vol. 17   page: 8 - 9   2015.3

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  36. Heterotrimeric G proteinsはCLAVATAシグナル伝達経路と協調的に機能し茎頂分裂組織における細胞増殖の制御を行う

    ISHIDA TAKASHI, TABATA RYO, YAMADA MASASHI, AIDA MITSUHIRO, MITSUMASU KANAKO, HIGUCHI MASAYUKI, TSUJI HIROYUKI, SHIMAMOTO KO, SAWA SHIN'ICHIRO

    日本植物生理学会年会要旨集   Vol. 56th   page: 172   2015.3

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  37. フロリゲン活性化複合体の細胞内イメージング

    TSUJI HIROYUKI

    植物の生長調節   Vol. 49 ( 2 ) page: 112 - 121   2014.12

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  38. 茎頂メリステムのメチローム解析から探るGene Body Methylationの意義

    TSUJI HIROYUKI, SAIHARA NORIKO, HIGASHI YOKO, MIURA FUMIHITO, KURATA TETSUYA, ITO TAKASHI, SHIMAMOTO KO

    育種学研究   Vol. 16   page: 90   2014.9

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  39. 全ゲノムバイサルファイトシーケンシングによる茎頂メリステムのメチローム解析

    TSUJI HIROYUKI, SAIHARA NORIKO, HIGASHI YOKO, MIURA FUMIHITO, ITO TAKASHI, TAMAKI SHOJIRO, KURATA TETSUYA, SHIMAMOTO KO

    育種学研究   Vol. 16   page: 99   2014.3

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  40. 植物ゲノム編集技術としての遺伝子ターゲティング:技術の現状とイネ耐病性関連遺伝子OsRac1の恒常活性化改変

    SHIMATANI ZEMPEI, TERADA RIE, DANG THU THI, KAWANO YOJI, TSUJI HIROYUKI, TAOKA KEN'ICHIRO, SHIMAMOTO KO

    育種学研究   Vol. 16   page: 124   2014.3

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  41. 茎頂メリステムのDNAメチローム解析

    TSUJI HIROYUKI, SAIHARA TOKUKO, HIGASHI YOKO, MIURA FUMIHITO, TAMAKI SHOJIRO, KURATA TETSUYA, ITO TAKASHI, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 55th   page: 226   2014.3

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  42. フロリゲン活性化複合体の新規転写因子OsFD2の機能解析

    TSUJI HIROYUKI, NAKAMURA HIROYUKI, TAOKA KEN'ICHIRO, SHIMAMOTO KO

    育種学研究   Vol. 15   page: 150   2013.10

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  43. 膜透過ペプチドを利用したイネフロリゲンタンパク質直接導入による人為的開花制御

    WASHIDA HARUHIKO, TSUJI HIROYUKI, OKI IZURU, TAOKA KEN'ICHIRO, SHIMAMOTO KO

    育種学研究   Vol. 15   page: 91   2013.10

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  44. イネフロリゲンHd3aによる花成促進機の解明

    TAMAKI SHOJIRO, TSUJI HIROYUKI, SAKAMOTO TOMOAKI, KURATA TETSUYA, SHIMAMOTO KO

    日本植物学会大会研究発表記録   Vol. 77th   page: 188   2013.8

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  45. キクの各発育相におけるCmFTとCmTFL1の発現解析

    ASAO HIROSHI, TSUJI HIROYUKI, SHIMAMOTO KO

    日本植物細胞分子生物学会大会・シンポジウム講演要旨集   Vol. 31st   page: 115   2013.8

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  46. キクへのフロリゲン(Hd3a)直接導入によるCmAP1の発現解析

    ASAO HIROSHI, TSUJI HIROYUKI, WASHIDA HARUHIKO, SHIMAMOTO KO

    育種学研究   Vol. 15   page: 63   2013.3

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  47. イネTFL1ホモログRCNによる花成抑制の分子機構の解析

    TAOKA KEN'ICHIRO, TERAKAWA CHIAKI, OKI IZURU, TSUJI HIROYUKI, KOJIMA CHOJIRO, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 54th   page: 215   2013.3

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  48. イネのフロリゲンHd3aと相互作用する転写因子OsFDの機能解析

    TSUJI HIROYUKI, NAKAMURA HIROYUKI, TAMAKI SHOJIRO, TAOKA KEN'ICHIRO, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 54th   page: 214   2013.3

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  49. イネ茎頂における栄養生長から生殖生長への相転換に関わる遺伝子の発現解析

    WATANABE YUTO, TAMAKI SHOJIRO, TSUJI HIROYUKI, SHIMAMOTO KO

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 36th   page: 1P-1061 (WEB ONLY)   2013

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  50. フロリゲンを中心とした転写活性化複合体FACの核移行メカニズムの解析

    NAKAMURA HIROYUKI, TSUJI HIROYUKI, SHIMAMOTO KO

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 36th   page: 2P-1069 (WEB ONLY)   2013

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  51. イネ茎頂メリステムのDNAメチローム解析

    TSUJI HIROYUKI, SAIHARA NORIKO, MIURA FUMIHITO, HIGASHI YOKO, TAMAKI SHOJIRO, KURATA TETSUYA, ITO TAKASHI, SHIMAMOTO KO

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 36th   page: 3P-0039 (WEB ONLY)   2013

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  52. 植物が花を咲かせる分子メカニズムの解明を目指して―NMR分光学から構造生命科学へ―

    TAOKA KEN'ICHIRO, OKI IZURU, TSUJI HIROYUKI, SHIMAMOTO KO, KOJIMA CHOJIRO

    日本分光学会テラヘルツ分光部会シンポジウム講演要旨集   Vol. 2013   page: 24 - 29   2013

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  53. 花成ホルモンフロリゲンと花芽形成遺伝子OsMADS15の同時イメージングによる花芽分化過程の解析

    FUJITA AKIKO, TSUJI HIROYUKI, MATSUMOTO AYANA, SHIMATANI ZEMPEI, TERADA RIE, SHIMAMOTO KO

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 36th   page: 3P-0558 (WEB ONLY)   2013

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  54. 花成ホルモン‐フロリゲン‐とその受容体の構造 解析からみえてきたフロリゲン機能の分子基盤

    TAOKA KEN'ICHIRO, OKI IZURU, TSUJI HIROYUKI, KOJIMA CHOJIRO, SHIMAMOTO KO

    化学と生物   Vol. 50 ( 9 ) page: 654 - 659   2012.9

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    DOI: 10.1271/kagakutoseibutsu.50.654

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  55. 花成ホルモン・フロリゲンの受容と機能のメカニズム

    TSUJI HIROYUKI, NAKASHIMA CHIKA, TAOKA KEN'ICHIRO, OKI IZURU, KOJIMA CHOJIRO, INADA NORIKO, SHIMAMOTO KO

    日本遺伝学会大会プログラム・予稿集   Vol. 84th   page: 78   2012.8

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  56. 花咲かホルモン(フロリゲン)

    TSUJI HIROYUKI, TAOKA KEN'ICHIRO, SHIMAMOTO KO

    植調   Vol. 46 ( 5 ) page: 179 - 186   2012.8

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  57. 膜透過ペプチドを利用した植物へのイネフロリゲンHd3aタンパク質直接導入

    WASHIDA HARUHIKO, TSUJI HIROYUKI, TAOKA KEN'ICHIRO, KOJIMA CHOJIRO, SHIMAMOTO KO

    育種学研究   Vol. 14   page: 144   2012.3

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  58. in vivo imaging of Florigen Activation Complex (FAC) comprising Hd3a, 14-3-3 and OsFD1 in rice

    TSUJI HIROYUKI, NAKASHIMA CHIKA, TAOKA KEN'ICHIRO, OKI IZURU, KOJIMA CHOJIRO, INADA NORIKO, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 53rd   page: 101   2012.3

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  59. イネ茎頂メリステムにおける花成最初期のグローバルRNA発現解析

    HIGASHI YOKO, TAMAKI SHOJIRO, TSUJI HIROYUKI, KURATA TETSUYA, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 53rd   page: 260   2012.3

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  60. フロリゲン活性化複合体FACの活性調節機構の解析

    TAOKA KEN'ICHIRO, OKI IZURU, TSUJI HIROYUKI, KOJIMA CHOJIRO, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 53rd   page: 205   2012.3

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  61. Florigen Hd3a protein acts as a mobile branching signal in rice.

    TSUJI HIROYUKI, TACHIBANA CHINATSU, TAMAKI SHOJIRO, TAOKA KEN'ICHIRO, KYOZUKA JUNKO, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 53rd   page: 205   2012.3

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  62. ジーンターゲティングにより作成したOsMADS15‐mOrangeトランスジェニックイネの解析

    MATSUMOTO AYANA, SHIMATANI ZEMPEI, TSUJI HIROYUKI, TERADA RIE, SHIMAMOTO KO

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 35th   page: 2P-0722 (WEB ONLY)   2012

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  63. フロリゲンによる花成の分子機構と花成以外の形態形成制御

    SHIMAMOTO KO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, OKI IZURU, KOJIMA CHOJIRO

    日本生化学会大会(Web)   Vol. 85th   page: 2S19-5 (WEB ONLY)   2012

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  64. 全ゲノムバイサルファイトシーケンス法による茎頂メリステムの生長相転換過程のメチローム解析

    SAIHARA NORIKO, TSUJI HIROYUKI, MIURA FUMIHITO, ITO TAKASHI, SHIMAMOTO KO

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 35th   page: 2P-0728 (WEB ONLY)   2012

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  65. 花成ホルモン・フロリゲンの受容と機能のメカニズム

    TSUJI HIROYUKI, TAOKA KEN'ICHIRO, OKI IZURU, TAMAKI SHOJIRO, NAKASHIMA CHIKA, NAKAMURA HIROYUKI, INADA NORIKO, KOJIMA CHOJIRO, SHIMAMOTO KO

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 35th   page: 2W7III-5 (WEB ONLY)   2012

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  66. 植物花成ホルモン(フロリゲン)受容体による花成制御機構の分子基盤

    OKI IZURU, FURUITA KYOKO, HAYASHI KOKORO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, NAKAGAWA ATSUSHI, SHIMAMOTO KO, KOJIMA CHOJIRO

    日本生化学会大会(Web)   Vol. 85th   page: 3T08-04 (WEB ONLY)   2012

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  67. イネフロリゲン遺伝子(Hd3a)導入キク‘神馬’における花成関連遺伝子の発現解析

    ASAO HIROSHI, TSUJI HIROYUKI, HASEBE CHIE, TAMAKI SHOJIRO, NAKA TERUFUMI, SHIMAMOTO KO

    園芸学研究 別冊   Vol. 10 ( 2 ) page: 237   2011.9

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  68. Hd3a‐14‐3‐3‐OsFD1からなる複合体Florigen Activation Complexの機能解析

    TSUJI HIROYUKI, TAOKA KEN'ICHIRO, OKI IZURU, NAKASHIMA CHIKA, KOJIMA CHOJIRO, SHIMAMOTO KO

    育種学研究   Vol. 13   page: 82   2011.9

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  69. 14-3-3タンパク質はフロリゲンの細胞内における受容体としてはたらく

    田岡健一郎, 大木出, 辻寛之, 児嶋長次郎, 島本功

    ライフサイエンス新着論文レビュー(http://first.lifesciencedb.jp/)     2011.9

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  70. 植物花成ホルモンフロリゲンによる花成誘導メカニズムの分子基盤

    OKI IZURU, FURUITA KYOKO, HAYASHI KOKORO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, NAKAGAWA ATSUSHI, SHIMAMOTO KO, KOJIMA CHOJIRO

    日本蛋白質科学会年会プログラム・要旨集   Vol. 11th   page: 120   2011.5

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  71. イネフロリゲン遺伝子(Hd3a)導入キク‘神馬’のロゼット相,幼若相および感光相での開花特性

    ASAO HIROSHI, HASEBE CHIE, TAMAKI SHOJIRO, NAKA TERUFUMI, TSUJI HIROYUKI, SHIMAMOTO KO

    園芸学研究 別冊   Vol. 10 ( 1 ) page: 215   2011.3

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  72. イネのフロリゲンHd3aと相互作用する転写因子OsFDの機能解析

    TSUJI HIROYUKI, TAMAKI SHOJIRO, TAOKA KEN'ICHIRO, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 52nd   page: 158   2011.3

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  73. イネフロリゲンHd3aタンパク質複合体の機能解析

    TAOKA KEN'ICHIRO, SHIMADA CHIHIRO, YANASE TOMOKO, OKI IZURU, TSUJI HIROYUKI, KOJIMA CHOJIRO, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 52nd   page: 158   2011.3

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  74. 植物細胞内におけるFlorigen Activation Complex(FAC)の解析

    NAKASHIMA CHIKA, TSUJI HIROYUKI, TAOKA KEN'ICHIRO, INADA NORIKO, SHIMAMOTO KO

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 34th   page: 4P-0303 (WEB ONLY)   2011

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  75. 植物花成ホルモン(フロリゲン)受容体による新たな花成誘導機構の分子基盤

    OKI IZURU, FURUITA KYOKO, HAYASHI KOKORO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, NAKAGAWA ATSUSHI, SHIMAMOTO KO, KOJIMA CHOJIRO

    日本分子生物学会年会プログラム・要旨集(Web)   Vol. 34th   page: 2T5A-4 (WEB ONLY)   2011

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  76. イネのフロリゲンHd3aと相互作用する転写因子OsFD1の機能解析

    TSUJI HIROYUKI, TAOKA KEN'ICHIRO, OKI IZURU, YAMAGUCHI MIDORI, OGAKI YUKA, PURWESTRI YEKTI ASIF, KOJIMA CHOJIRO, SHIMAMOTO KO

    育種学研究   Vol. 12   page: 133   2010.9

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  77. 遺伝子組み換えによらない植物細胞へのタンパク質直接導入

    WASHIDA HARUHIKO, TSUJI HIROYUKI, TAOKA KEN'ICHIRO, KOJIMA CHOJIRO, SHIMAMOTO KO

    育種学研究   Vol. 12   page: 215   2010.9

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  78. イネフロリゲンタンパク質Hd3aの花成制御機構の構造基盤

    OKI IZURU, FURUITA KYOKO, TAOKA KEN'ICHIRO, HAYASHI KOKORO, FUKADA HARUMI, TSUJI HIROYUKI, NAKAGAWA ATSUSHI, SHIMAMOTO KO, KOJIMA CHOJIRO

    日本蛋白質科学会年会プログラム・要旨集   Vol. 10th   page: 151   2010.5

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  79. イネフロリゲンHd3aタンパク質と相互作用する転写因子OsFD1の機能解析

    TSUJI HIROYUKI, TAOKA KEN'ICHIRO, OKI IZURU, YAMAGUCHI MIDORI, OGAKI YUKA, KOJIMA CHOJIRO, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 51st   page: 214   2010.3

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  80. イネ半優性矮性変異体Slr1‐dの解析

    HIRANO KO, ASANO KENJI, KAMIGUCHI(TANAKA) MIYAKO, TSUJI HIROYUKI, KAMURA TOSHIRO, SATO HIKARU, KITANO HIDEMI, ASHIKARI MOTOYUKI, MATSUOKA MAKOTO

    日本植物生理学会年会要旨集   Vol. 51st   page: 124   2010.3

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  81. イネフロリゲンHd3aタンパク質の茎頂分裂組織における挙動

    TAMAKI SHOJIRO, TSUJI HIROYUKI, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 51st   page: 213   2010.3

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  82. イネフロリゲンHd3aタンパク質の機能ドメイン解析

    TAOKA KEN'ICHIRO, SHIMADA CHIHIRO, YANASE TOMOKO, OKI IZURU, TSUJI HIROYUKI, KOJIMA CHOJIRO, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 51st   page: 214   2010.3

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  83. イネフロリゲンHd3aタンパク質の分子機能の構造的基盤

    OKI IZURU, HAYASHI KOKORO, FURUITA KYOKO, FUKADA HARUMI, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, SHIMAMOTO KO, KOJIMA CHOJIRO

    日本植物生理学会年会要旨集   Vol. 51st   page: 214   2010.3

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  84. イネのフロリゲンHd3aタンパク質と相互作用する転写因子OsKANADI1の機能解析

    UCHIMURA SHOHEI, TSUJI HIROYUKI, PURWESTRI YEKTI ASIH, YANASE TOMOKO, TAOKA KEN'ICHIRO, SHIMAMOTO KO

    生化学     page: ROMBUNNO.2P-1070   2010

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  85. イネフロリゲンHd3aによる遺伝子発現制御機構の解析

    SHIMADA CHIHIRO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, SHIMAMOTO KO

    生化学     page: ROMBUNNO.2P-1071   2010

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  86. イネフロリゲン遺伝子(Hd3a)導入キク‘神馬’における低温遭遇が開花に及ぼす影響

    ASAO HIROSHI, HASEBE CHIE, TAMAKI SHOJIRO, NAKA TERUFUMI, TSUJI HIROYUKI, SHIMAMOTO KO

    園芸学研究 別冊   Vol. 8 ( 2 ) page: 286   2009.9

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  87. イネのフロリゲンHd3aタンパク質による分げつの促進

    TSUJI HIROYUKI, TACHIBANA CHINATSU, TAMAKI SHOJIRO, KOMIYA REINA, SHIMAMOTO KO

    育種学研究   Vol. 11   page: 55   2009.9

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  88. ターゲットタンパク研究プログラムの成果 花成ホルモン,フロリゲン その構造解析から分子機構の理解へ

    TAOKA KEN'ICHIRO, TSUJI HIROYUKI, SHIMAMOTO KO

    蛋白質 核酸 酵素   Vol. 54 ( 12 ) page: 1702 - 1707   2009.9

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  89. Structural aspects of florigen to understand the molecular mechanism of flowering

    田岡 健一郎, 辻 寛之, 島本 功

    Protein, nucleic acid and enzyme   Vol. 54 ( 12 ) page: 1702 - 1707   2009.9

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  90. イネのジベレリンシグナル伝達の初期過程にかかわるGID1,SLR1,GID2の相互作用

    HIRANO KO, TSUJI HIROYUKI, KAMIGUCHI(TANAKA) MIYAKO, MATSUOKA MAKOTO

    日本植物生理学会年会要旨集   Vol. 50th   page: 173   2009.3

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  91. イネ花成ホルモンHd3aタンパク質の移動メカニズムの解析

    TAMAKI SHOJIRO, PURWESTRI YEKTI ASIH, TSUJI HIROYUKI, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 50th   page: 209   2009.3

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  92. イネのフロリゲンHd3aによる分枝の促進

    TSUJI HIROYUKI, TACHIBANA CHINATSU, TAMAKI SHOJIRO, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 50th   page: 209   2009.3

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  93. PVXベクターを用いたイネフロリゲンHd3aのタバコにおける機能評価系の確立

    MURAKAMI YUKIKO, TAOKA KEN'ICHIRO, TSUJI HIROYUKI, SHIMAMOTO KO

    日本分子生物学会年会講演要旨集   Vol. 32nd ( Vol.4 ) page: 179   2009

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  94. イネ花成ホルモンHd3aはジャガイモの塊茎形成を誘導する

    TAMAKI SHOJIRO, NAVARRO CRISTINA, YOKOI SHUJI, TSUJI HIROYUKI, PRAT SALOME, SHIMAMOTO KO

    日本植物生理学会年会要旨集   Vol. 49th   page: 207   2008.3

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  95. ジベレリンシグナルに関与するMYB型転写因子GAMYBの葯における機能

    AYA KOICHIRO, KAMIGUCHI(TANAKA) MIYAKO, TSUJI HIROYUKI, KONDO MAKI, NISHIMURA MIKIO, ASHIKARI MOTOYUKI, MATSUOKA MAKOTO

    日本植物生理学会年会要旨集   Vol. 49th   page: 190   2008.3

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  96. イネのフロリゲンHd3aの相互作用する14‐3‐3タンパク質の解析

    YANASE TOMOKO, OGAKI YUKA, PURWESTRI YEKTI ASIH, TAOKA KEN'ICHIRO, WONG HANN LING, TSUJI HIROYUKI, SHIMAMOTO KO

    生化学     page: 3P-1238   2008

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  97. イネの移動性花成シグナルHd3aは花以外の形態形成にも関与する

    TACHIBANA CHIKA, TSUJI HIROYUKI, TAMAKI SHOJIRO, SHIMAMOTO KO

    生化学     page: 1P-1109   2007

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  98. フロリゲンの解明と次の謎

    SHIMAMOTO KO, TAMAKI SHOJIRO, MATSUO SHOICHI, WONG HANN LING, YOKOI SHUJI, TACHIBANA CHIKA, TSUJI HIROYUKI, ASAO HIROSHI

    生化学     page: 3W14-5   2007

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  99. Analysis of expression of genes for mitochondrial aldehyde dehydrogenase in maize during submergence and following re-aeration Reviewed

    Naoki Meguro, Hiroyuki Tsuji, Yasuhiro Suzuki, Nobuhiro Tsutsumi, Atsushi Hirai, Mikio Nakazono

    BREEDING SCIENCE   Vol. 56 ( 4 ) page: 365 - 370   2006.12

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    Language:English   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)   Publisher:JAPANESE SOC BREEDING  

    Acetaldehyde is oxidized from anaerobically accumulated ethanol during re-aeration in plants and may cause post-anoxic (post-hypoxic) injury. Aldehyde dehydrogenase (ALDH) can metabolize acetaldehyde to acetate, which is much less toxic. To understand the role of ALDH in alleviating post-anoxic injury of plants, we analyzed the expression of the mitochondrial ALDH genes, rf2a and rf2b, and determined the ethanol and acetaldehyde contents under submergence and subsequent re-aeration in a submergence-intolerant plant, maize (Zea mays L., inbred line B73). The level of rf2a mRNA decreased, whereas that of rf2b mRNA increased under submerged conditions. When the submerged plants were re-aerated, the transcript levels returned to the original levels. The level of mitochondrial ALDH proteins decreased under submergence and remained unchanged under re-aeration. The ALDH activity decreased under submergence and, during reaeration, recovered to the original level much more slowly than in the case of rice (Oryza sativa L.). The content of acetaldehyde, which was produced under submergence, further increased following re-aeration. These results suggest that the lower submergence tolerance of maize compared to that of rice is partly due to a weaker ALDH activity during re-aeration.

    DOI: 10.1270/jsbbs.56.365

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  100. ジベレリンシグナル伝達制御因子SLR1,GID1及びGID2の相互作用

    TSUJI HIROYUKI, KAMIGUCHI(TANAKA) MIYAKO, NAKAJIMA MASATOSHI, ASHIKARI MOTOYUKI, KITANO HIDEMI, YAMAGUCHI ISOMARO, MATSUOKA MAKOTO

    日本植物生理学会年会要旨集   Vol. 47th   page: 206   2006.3

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  101. マイクロアレイによるイネの葯特異的遺伝子の解析‐gamyb変異体において発現が変動する遺伝子群について

    AYA KOICHIRO, KAMIGUCHI(TANAKA) MIYAKO, KONDO MAKI, TSUJI HIROYUKI, ASHIKARI MOTOYUKI, NISHIMURA MIKIO, MATSUOKA MAKOTO

    日本植物生理学会年会要旨集   Vol. 47th   page: 325   2006.3

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  102. イネマイクロアレイによるやく特異的遺伝子の解析―gamyb変異体において発現が変動する遺伝子群について

    AYA KOICHIRO, UEGUCHI(TANAKA) MIYAKO, TSUJI HIROYUKI, ASHIKARI MOTOYUKI, MATSUOKA MAKOTO

    日本植物生理学会年会要旨集   Vol. 46th   page: 292   2005.3

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  103. ジベレリンシグナル伝達における核内転写因子の機能と制御

    TSUJI HIROYUKI, UEGUCHI(TANAKA) MIYAKO, ASHIKARI MOTOYUKI, KITANO HIDEMI, MATSUOKA MAKOTO

    日本分子生物学会年会プログラム・講演要旨集   Vol. 27th   page: 386   2004.11

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  104. Expression analysis of histone deacetylase in rice

    H Tsuji, H Saika, N Tsutsumi, M Nakazono

    PLANT AND CELL PHYSIOLOGY   Vol. 44   page: S53 - S53   2003

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Presentations 10

  1. 植物リソース配分の成長相転換 Invited

    辻寛之1,2(1 名古屋大学・生物機能開発利用研究センター,2 横浜市立大学・木原生物学研究所)

    第64回日本植物生理学会年会シンポジウム「リソース配分制御から読み解く植物の生存戦略」  2023.3.15  日本植物生理学会

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    Event date: 2023.3

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:東北大学   Country:Japan  

  2. イネのシュート頂メリステムにおける微小管のイメージング系の確立

    髙田 崚輔1、井藤 純1、辻 寛之1(1. 横浜市立大・木原生物学研究所)

    日本育種学会第142回講演会(令和4年度秋季大会)  2022.9.24  日本育種学会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:帯広畜産大学   Country:Japan  

  3. オオムギのユニークな花序構造に関する遺伝子発現解析

    井藤 純1、佐藤 奈緒1、野村 有子1、新井 駿一1、高萩 航太郎2、岡田 聡史3,4、武田(神谷) 紀子2、豊岡 公徳2、最相 大輔3、平山 隆志3、持田 恵一2、辻 寛之1,4 (1. 横浜市大・木原生物学研究所、2. 理研・環境資源科学研究センター、3. 岡山大・資源植物科学研究所、4. 名古屋大・生物機能開発利用研究センター)

    日本育種学会第142回講演会(令和4年度秋季大会)  2022.9.24  日本育種学会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:帯広畜産大学   Country:Japan  

  4. オオムギ花序メリステムの細胞死過程における細胞内構造変化

    松本 大輝1、井藤 純1、新井 駿一1、野村 有子1、杉村 みどり1、佐藤 奈緒1、関 緑1、若崎 眞由美2、佐藤 繭子2、武田(神谷) 紀子2、宇野 何岸4,5、佐藤 良勝4,6、最相 大輔7、豊岡 公徳2、辻 寛之1,3 (1. 横浜市大・木原生物学研究所、2. 理研・環境資源科学研究センター、3. 名古屋大学生物機能開発利用研究センター、4. 名古屋大学理学研究科、5. Department of NanoBiophotonics, Max Planck Institute for Multidisciplinary Sciences (MPI-NAT)、6. 名古屋大学トランスフォーマティブ生命分子研究所(WPI-ITbM)、7. 岡山大学資源植物科学研究所)

    日本育種学会第142回講演会(令和4年度秋季大会)  2022.9.24  日本育種学会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:帯広畜産大学   Country:Japan  

  5. オオムギの葉鞘基部に「窓」を開け、発生中の穂を外科的にカットするとどうなるか

    田中 真理1、赤司 裕子1、○辻 寛之1,2 (1. 横浜市大・木原生研、2. 名古屋大・生物機能開発利用研究センター)

    日本育種学会第142回講演会(令和4年度秋季大会)  2022.9.24  日本育種学会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:帯広畜産大学   Country:Japan  

  6. フロリゲンの分子機能 Invited

    辻 寛之

    IPSRセミナー  2022.6.16  岡山大学資源植物科学研究所

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    Event date: 2022.6

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:岡山大学資源植物科学研究所   Country:Japan  

  7. Florigen function and epigenomic regulation during floral transition Invited International conference

    Hiroyuki Tsuji

    2017.3.17 

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    Language:English   Presentation type:Oral presentation (invited, special)  

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  8. Molecular function of florigen Invited

    Tsuji, H.

    PSC seminar, Shanghai Center for Plant Stress Biology, Chinese Academy of Sciences  2019.11.7 

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    Language:English   Presentation type:Oral presentation (keynote)  

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  9. Molecular function of florigen Invited

    Tsuji, H.

    PSC seminar  2019.11.8 

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    Language:English   Presentation type:Oral presentation (keynote)  

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  10. Imaging florigen distribution in the shoot apical meristem Invited

    Tsuji, H.

    17th International Symposium on Rice Functional Genomics (ISRFG2019)  2019.11.5 

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Research Project for Joint Research, Competitive Funding, etc. 9

  1. 持続的な食糧生産を可能にする野生植物の新奇食糧資源化

    Grant number:JPMJMI23C1  2024.4 - 2028.3

    未来社会創造事業 

    ツジヒロユキ

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    Authorship:Coinvestigator(s) 

  2. 茎頂メリステムにおけるフロリゲンの分子機能解明

    Grant number:21H04728  2021.4 - 2025.3

    日本学術振興会  科学研究費補助金  基盤研究(A)

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    Authorship:Principal investigator  Grant type:Competitive

  3. 野生遺伝資源を活用したイネ科新奇食糧資源の開拓 研究課題

    Grant number:JPMJMI20C8  2020.8 - 2023.3

    未来社会創造事業  探索加速型

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    Authorship:Coinvestigator(s)  Grant type:Competitive

  4. データ科学に基づく作物設計基盤技術の構築

    Grant number:JPMJCR16O4  2016.8 - 2021.3

    戦略的創造研究推進事業  CREST

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    Authorship:Coinvestigator(s)  Grant type:Competitive

  5. 花成ホルモン・フロリゲンを起点とする花形成の「鍵と鍵穴」相互作用の解明

    Grant number:16H06466  2016.8 - 2021.3

    新学術領域研究 (研究領域提案型)  計画研究

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    Authorship:Principal investigator  Grant type:Competitive

  6. フロリゲン活性化複合体の分子機能解明

    Grant number:16H02532  2016.4 - 2020.3

    科学研究費補助金  基盤研究(A)

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    Authorship:Principal investigator 

  7. 我が国を拠点とした実用作物の世界最先端ゲノム編集研究国際ネットワークの構築 International coauthorship

    Grant number:S2804  2016.4 - 2020.3

    人材育成事業  頭脳循環を加速する戦略的国際研究ネットワーク推進プログラム

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    Authorship:Principal investigator  Grant type:Competitive

  8. フロリゲンを活用して地球温暖化に強い作物を作るための基礎研究

    2016.4 - 2019.3

    キャノン財団研究助成  「理想の追求」

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    Authorship:Principal investigator  Grant type:Competitive

  9. フロリゲン活性化複合体の形成メカニズムの解明と開花調節への応用

    Grant number:24688001  2012.4 - 2015.3

    日本学術振興会  科学研究費補助金  若手研究(A)

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    Authorship:Principal investigator  Grant type:Competitive

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KAKENHI (Grants-in-Aid for Scientific Research) 19

  1. フロリゲンの転写活性化能を制御する化合物による花成制御

    Grant number:22K19184  2022.6 - 2024.3

    科学研究費助成事業  挑戦的研究(萌芽)

    辻 寛之, 児嶋 長次郎

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    Authorship:Principal investigator 

    Grant amount:\6500000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

    植物に普遍的な花成ホルモンであるフロリゲン(正体はFT/Hd3aタンパク質)が作用を発揮する上ではフロリゲン活性化複合体形成を介した「転写活性化能」の獲得が最も重要なステップであると考えられている。これを制御する化合物を発見できれば、従来不可能であった化合物投与による開花時期制御への道が切り開かれる。本研究ではフロリゲン活性化複合体の転写活性化能に焦点を絞り、フロリゲン活性化複合体の機能制御化合物の探索と化合物を活用したフロリゲンの機能制御技術を開拓する。

  2. Molecular mechanisms linking floral transition and stem elongation in plants

    Grant number:22H04978  2022.4 - 2027.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (S)

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    Authorship:Coinvestigator(s) 

  3. Determining principles in the birth of new plant species: elucidation of lock-and-key molecular systems in sexual reproduction

    Grant number:21H00458  2021.4 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

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    Authorship:Coinvestigator(s) 

  4. データ科学に基づく作物設計基盤技術の構築

    2016.10 - 2022.3

    JST  戦略的創造研究推進事業・CREST 

    辻 寛之

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    Authorship:Principal investigator  Grant type:Competitive

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  5. 我が国を拠点とした実用作物の世界最先端ゲノム編集研究国際ネットワークの構築

    2016.10 - 2019.3

    JST  頭脳循環プログラム 

    辻 寛之

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    Authorship:Principal investigator  Grant type:Competitive

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  6. 植物新種誕生の原理-生殖過程の鍵と鍵穴の分子実態解明を通じて 総括班事務局

    2016.7 - 2021.3

    日本学術振興会  科学研究費補助金・新学術領域研究・総括班 

    辻 寛之

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    Authorship:Principal investigator  Grant type:Competitive

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  7. 花成ホルモン・フロリゲンを起点とする花形成の「鍵と鍵穴」相互作用の解明

    2016.7 - 2021.3

    日本学術振興会  科学研究費補助金・新学術領域研究・計画研究 

    辻 寛之

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    Authorship:Principal investigator  Grant type:Competitive

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  8. Principles of the Birth of New Plant Species - Center for Supporting International Activities Toward the Formation of an International Research Core -

    Grant number:16K21727  2016.6 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Higashiyama Tetsuya

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    Authorship:Coinvestigator(s) 

    (1) To form an international research core, we invited more than 200 researchers and students from all over the world, including those from partner overseas research centers such as UC Davis in the U.S. and the University of Zurich in Switzerland, mainly to ITbM at Nagoya University and the Kihara Institute of Biological Sciences at Yokohama City University. (2) Dispatch of young researchers and students, mainly to North American and European research centers, led to a number of international coauthored papers. (3) As an information dissemination strategy to enhance international visibility, we held Japan's first International Conference on Sexual Plant Reproduction and EMBO Practical Course with experiments, and published many open-access papers in three special issues.

  9. Determining principles in the birth of new plant species: elucidation of lock-and-key molecular systems in sexual reproduction

    Grant number:16H06464  2016.6 - 2021.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Higashiyama Tetsuya

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    Authorship:Coinvestigator(s) 

    The Support Center for Interdisciplinary Researches promoted interdisciplinary researches of the project. In terms of the management of the project, the leadership of Dr. Higashiyama, who is the director of the project, was exercised so that the smooth and active management of the project was carried out. Many joint researches were conducted through the network established through the activities of the project. The website of the project, which receives a high number of hits, was used to promptly disseminate research results, including the publication of research papers. The research results were returned to the public and disseminated to the world through the International Conference on Sexual Plant Reproduction and the EMBO practical course with experiments held for the first time in Japan, and many outreach activities.

  10. フロリゲン活性化複合体の分子機能解明

    2016.4 - 2020.3

    日本学術振興会  科学研究費補助金・基盤研究(A) 

    辻 寛之

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    Authorship:Principal investigator  Grant type:Competitive

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  11. フロリゲンを活用して地球温暖化に強い作物を作るための基礎研究

    2016.4 - 2019.3

    キヤノン財団  研究助成「理想の追求」 

    辻 寛之

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    Authorship:Principal investigator  Grant type:Competitive

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  12. Crop improvement by synthetic strategy using anti-florigen genes

    Grant number:16K14829  2016.4 - 2018.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    Tsuji Hiroyuki

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    Authorship:Principal investigator 

    Grant amount:\3640000 ( Direct Cost: \2800000 、 Indirect Cost:\840000 )

    Florigen and antiflorigen function in antagonistic manner for flowering and inflorescence development. Florigen promotes flowering and early termination of inflorescence branching that leads to the reduction of seed number, whereas anti-florigen have opposite phenotype. Overexpression of anti-florigen genes results in the increase in the number of seeds, whereas it causes delay in flowering which should be avoided in the breeding process. In this study we tried to overcome this problem by expressing anti-florigen genes under the control of the promoters that activated in the shoot apical meristem after the floral transition. We developed transgenic lines expressing synthetic anti-florigen genes under PAP2 promoter, and examined their phenotype. We found that several lines showed increase in the number of primary branches without delay in flowering. However total seed number was not increased in these lines, suggesting requirement for further improvement of our construct.

  13. Regulation of stomatal opening in response to environmental signals

    Grant number:15H05956  2015.6 - 2020.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Kinoshita Toshinori

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    Authorship:Coinvestigator(s) 

    Using stomatal guard cells, which are model cells of environmental response in plants, as the main material, we aimed to identify novel factors involved in signal transduction in response to environmental signals. As a result, we found novel factors related to control of stomatal aperture, the relationship between stomatal response and environmental memory of plants in response to environmental stimuli, and regulation of aperture via long-distance signals in response to environmental stimuli.

  14. Live imaging of florigen

    Grant number:26660006  2014 - 2015

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

    Tsuji Hiroyuki

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\3900000 ( Direct Cost: \3000000 、 Indirect Cost:\900000 )

    Florigen is a systemic signal that induces flowering in plants, and its molecular nature is a protein product encoded by FT/Hd3a gene. FT/Hd3a is expressed in leaf phloem tissue, and transported into the shoot apical meristem where it initiates floral transition. The important question in this process is how this protein transported through such a long distance, and especially how it reaches the shoot apical meristem. We tried to address these questions by live imaging of shoot apical meristem of the transgenic rice plants expressing Hd3a-GFP from its own promoter. Detailed observation of the shoot apical meristem revealed that Hd3a is continuasly transported from the beggining of the floral transition to the later stage of panicel development of plants. Our observation will help further understainding of how plants initiates reproductive development by florigen.

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  15. 植物幹細胞の生殖生長相転換とエピゲノムの相互作用

    Grant number:26113713  2014 - 2015

    文部科学省  科学研究費補助金(新学術領域研究(研究領域提案型))  新学術領域研究(研究領域提案型)

    辻 寛之

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\19890000 ( Direct Cost: \15300000 、 Indirect Cost:\4590000 )

    生長相転換に伴うトランスポゾンのサイレンシングのメカニズムについて理解を深めるために、野生型イネの栄養生長メリステムと生殖生長メリステムのDNAメチローム解析を実施した。その結果、CHH配列(HはC以外の塩基)におけるシトシンのメチル化が生殖生長期においてゲノム全体で上昇することが分かった。Small RNA-seq, RNA-seq, プロテオミクスを組み合わせて解析した結果、この制御がRNAiのシステム を介していることが分かった。
    27年度が最終年度であるため、記入しない。
    27年度が最終年度であるため、記入しない。

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  16. Molecular Mechanism of Florigen Function and Application of Florigen to Crop Improvement

    Grant number:24000017  2012 - 2016

    Ministry of Education, Culture, Sports, Science and Technology  Grants-in-Aid for Scientific Research(特別推進研究)  特別推進研究

    Ko SHIMAMOTO, 大木 出, Ken-ichiro TAOKA, Rie TERADA, Chojiro KOJIMA, Hiroyuki TSUJI, Izuru OHKI, Shojiro TAMAKI, TAOKA Ken-ichiro, TERADA Rie, KOJIMA Chojiro, TSUJI Hiroyuki, OHKI Izuru, TAMAKI Shojiro

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type:Competitive

    Comprehensive analyses of florigen, a flowering hormone, were performed to elucidate the molecular function of florigen. Accumulation of rice florigen Hd3a at shoot apical meristem (SAM), details of the structure of florigen activation complex (FAC), complex formation and its structure of rice antiflorigen RCN, transcriptome and methylome of SAM, potato tuberization control by FAC, were revealed. The potential for improvement of biomass and crop yield by manipulation of florigen was examined.

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  17. Functional analysis of TCP family transcription factors TCP8 and TCP14 that interact with rice florigen Hd3a.

    Grant number:21780004  2009 - 2010

    Ministry of Education, Culture, Sports, Science and Technology  Grants-in-Aid for Scientific Research(若手研究(B))  若手研究(B)

    Hiroyuki TSUJI

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

    Florigen was proposed about 70 years ago that produced in the leaves and moves up to shoot apex to induce flowering. In 2007 the molecular nature of florigen was revealed to be a family of FT/Hd3a protein. Hd3a protein itself does not possess any biochemically defined functional motifs thus Hd3a should interact with functional effectors to exert its function. Here, we found TCP transcription factors interact with Hd3a, and functional analysis of these proteins revealed their contribution to flowering.

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  18. Molecular mechanism of flowering in the short-day plant rice.

    Grant number:19060013  2007 - 2012

    Ministry of Education, Culture, Sports, Science and Technology  Grants-in-Aid for Scientific Research(特定領域研究)  特定領域研究

    Ko SHIMAMOTO, 辻 寛之, Rie TERADA, Izuru OHKI, Hiroyuki TSUJI, TERADA Rie, OHKI Izuru, TSUJI Hiroyuki

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type:Competitive

    Florigen is a mobile flowering signal produced in leaves and moves to the shoot apex. The molecular nature of florigen is revealed in 2007, as the protein product encoded by the Heading date 3a/FLOWERING LOCUS T genes in plants. Our molecular genetic analyses revealed the 14-3-3 proteins as the intracellular receptors of rice Hd3a florigen, and florigen activation complex, comprised of Hd3a, 14-3-3 and the transcription factor FD promotes flowering.

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  19. Functional analysis of rice flowering genes on the vegetative growth of rice.

    Grant number:19780003  2007 - 2008

    Ministry of Education, Culture, Sports, Science and Technology  Grants-in-Aid for Scientific Research(若手研究(B))  若手研究(B)

    Hiroyuki TSUJI

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\3880000 ( Direct Cost: \3400000 、 Indirect Cost:\480000 )

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Teaching Experience (On-campus) 19

  1. 資源生物科学実験実習2

    2024

  2. 資源生物科学実験実習1

    2024

  3. 植物ゲノム利用学

    2024

  4. 生命農学研究科特別講義

    2024

  5. 植物分子育種学特論

    2024

  6. 生命科学入門B

    2024

  7. 植物生理学2

    2024

  8. 植物研究アプローチ特論

    2024

  9. 資源生物科学実験実習2

    2023

  10. 資源生物科学実験実習2

    2023

  11. 資源生物科学実験実習1

    2023

  12. 植物ゲノム利用学

    2023

  13. 資源生物科学実験実習2

    2022

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    実験室内での組織・形態学、発生学、動物生理学、植物生理学、遺伝学に関する実験から東郷フィールドにおける生物生産実習、および農業経済学実習まで幅広い実験や実習を行う。

  14. 植物ゲノム利用学

    2022

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    3年次までに習得した専門知識を踏まえ,各研究室における卒業論文研究にいたるまでの橋渡しとなるような項目について講義や実習を行う。「植物ゲノム利用学」では,近年,急増している植物ゲノムビックデータを利用した育種学研究分野についての知識を習得する。「アイソトープ実験法」では,本学において卒業研究等でラジオアイソトープを使用するための利用資格を得ることを目的として,ラジオアイソトープ取り扱いの基礎知識と技術を学ぶ。「国際農学演習」では,「海外実地研修」および「海外学生受入研修」を受講するにあたり,グループワークと英語での発表を通して,研修先についての基礎知識を身に付ける。「生物データベース利用演習」では,ゲノムデータベース取扱の初歩について,PCを用いた講義・演習を行う。

  15. 生命農学序説

    2022

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    この講義の目的は,「生命農学における学術・研究の概要」,「生命農学の社会的使命」,「今後履修する基礎および専門科目の必要性」,「習得した知識・技能の将来展開」を理解することである。また,各学科の理念や各研究室の研究背景・現状・将来を知ることで,今後4年間の学修の方向性や取り組み方を考えるきっかけを得る。生命農学に対する知的好奇心を高めるとともに,科学,技術,社会への視野を広げることによって,勉学意欲の向上・今後の大学生活への期待を一層高める。

  16. 植物生理学2

    2022

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    植物生理学2では,植物の個体形成と分化の柔軟性について学ぶ。また,植物の発生・成長・分化の制御機構に関する理解を深める。すなわち,胚発生から種子をへて栄養成長に至る過程、栄養成長から生殖成長への転換と受精、結実に至る各項目を習得する。

  17. 資源生物科学実験実習1

    2022

  18. 植物ゲノム利用学

    2022

  19. 資源生物科学実験実習2

    2022

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Teaching Experience (Off-campus) 11

  1. 課題提案型演習Bb

    2020.4 Yokohama City University)

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    Level:Undergraduate (specialized) 

  2. 課題提案型演習Ab

    2020.4 Yokohama City University)

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    Level:Undergraduate (specialized) 

  3. 基礎ゼミ

    2017.4 Yokohama City University)

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    Level:Undergraduate (liberal arts) 

  4. 生物学概説A

    2015.4 Yokohama City University)

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    Level:Undergraduate (liberal arts)  Country:Japan

  5. 生命環境システム科学特別研究

    2015.4 Yokohama City University)

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    Level:Postgraduate  Country:Japan

  6. 卒業論文

    2015.4 Yokohama City University)

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    Level:Undergraduate (specialized)  Country:Japan

  7. 卒業研究II

    2015.4 Yokohama City University)

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    Level:Undergraduate (specialized)  Country:Japan

  8. 卒業研究Ⅰ

    2015.4 Yokohama City University)

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    Level:Undergraduate (specialized)  Country:Japan

  9. 遺伝資源科学特論

    2015.4 Yokohama City University)

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    Level:Postgraduate  Country:Japan

  10. 生命環境システム科学概説I

    2015.4 Yokohama City University)

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    Level:Postgraduate  Country:Japan

  11. ゲノム遺伝学

    2015.4 Yokohama City University)

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    Level:Undergraduate (specialized)  Country:Japan

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Social Contribution 1

  1. フロリゲンの分子機能

    Role(s):Lecturer

    麻布高等学校  2022.5