Updated on 2025/03/18

写真a

 
Ryo Kinoshita-Daitoku
 
Organization
Graduate School of Medicine Program in Integrated Medicine Microbiology and Immunology Assistant Professor
Graduate School
Graduate School of Medicine
Undergraduate School
School of Medicine Department of Medicine
Title
Assistant Professor

Degree 1

  1. 博士 (医学) （ 2019.3   東京大学 ） 

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Research History 2

  1. Osaka University   Assistant Professor

    2020.6 - 2021.6

  2. Osaka University   Researcher

    2019.4 - 2020.5

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Papers 9

  1. A bacterial small RNA regulates the adaptation of Helicobacter pylori to the host environment. Reviewed

    Ryo Kinoshita-Daitoku, Kotaro Kiga, Masatoshi Miyakoshi, Ryota Otsubo, Yoshitoshi Ogura, Takahito Sanada, Zhu Bo, Tuan Vo Phuoc, Tokuju Okano, Tamako Iida, Rui Yokomori, Eisuke Kuroda, Sayaka Hirukawa, Mototsugu Tanaka, Arpana Sood, Phawinee Subsomwong, Hiroshi Ashida, Tran Thanh Binh, Lam Tung Nguyen, Khien Vu Van, Dang Quy Dung Ho, Kenta Nakai, Toshihiko Suzuki, Yoshio Yamaoka, Tetsuya Hayashi, Hitomi Mimuro

    Nature communications     2021.4

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： https://doi.org/10.1038/s41467-021-22317-7

  2. A nationwide survey of antimicrobial resistance of Escherichia coli isolated from broiler chickens in Malawi Open Access

    Chisembe, P; Suzuki, M; Dao, DT; Njunga, G; Nkhoma, J; Mthilakuwili, L; Kinoshita-Daitoku, R; Kuroda, E; Kimura, K; Shibayama, K

    JAC-ANTIMICROBIAL RESISTANCE   Vol. 6 ( 6 ) page： dlae200   2024.12

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    Language：English   Publisher：JAC-Antimicrobial Resistance  

    Background: Antimicrobial resistance is a global health challenge with profound implications across sectors. Livestock, a significant field at the One Health interface, lacks sufficient information, particularly in low-resource settings such as Malawi. Objectives: We determined the antimicrobial resistance rates of Escherichia coli isolated from broiler chickens in Malawi and explored the relationship between resistance genes across sectors using genomic analysis. Methods: In 2023, we isolated 115 E. coli strains from 116 faecal and caecal samples from broiler chickens across Malawi. Antimicrobial susceptibility tests were performed using agar dilution method according to the Clinical Laboratory Standard Institute guidelines. Whole-genome sequencing was performed using Illumina sequencing. Results: Notably, 50 isolates (44%) were resistant to cefotaxime. We detected ESBL blaCTX-M genes (blaCTX-M-55, blaCTX-M-14, blaCTX-M-65, blaCTX-M-27, blaCTX-M-15, blaCTX-M-1, and blaCTX-M-3) in 48 cefotaxime-resistant isolates, which exhibited higher resistance rates to levofloxacin than non-ESBL-encoding isolates (29/48; 60% versus 20/67; 30%). All isolates were susceptible to colistin and carbapenems. High resistance rates were observed for tetracycline and co-trimoxazole commonly used in broiler chickens (90% and 70%, respectively). Sequence type 206 and phylogroup A were predominant (14% and 65%, respectively). In the genetic context of blaCTX-M genes, whole-genome alignment of the ESBL-producing isolates with reference plasmids from E. coli of various origins indicated significant similarity. Conclusions: Antimicrobial resistance is highly prevalent among E. coli from broiler chickens in Malawi. Genomic analysis suggests potential transmission pathways for ESBL genes across sectors, necessitating further studies from One Health perspective.

    DOI： 10.1093/jacamr/dlae200

    Open Access

    Web of Science

    Scopus

    PubMed

  3. Effect of low oxygen concentration on activation of inflammation by Helicobacter pylori. Reviewed

    Abass A, Okano T, Boonyaleka K, Kinoshita-Daitoku R, Yamaoka S, Ashida H, Suzuki T.

    Biochem Biophys Res Commun.     2021.5

  4. Group A Streptococcus establishes pharynx infection by degrading the deoxyribonucleic acid of neutrophil extracellular traps Reviewed

    Mototsugu Tanaka, Ryo Kinoshita-Daitoku, Kotaro Kiga, Takahito Sanada, Bo Zhu, Tokuju Okano, Chihiro Aikawa, Tamako Iida, Yoshitoshi Ogura, Tetsuya Hayashi, Koshu Okubo, Miho Kurosawa, Junichi Hirahashi, Toshihiko Suzuki, Ichiro Nakagawa, Masaomi Nangaku, Hitomi Mimuro

    Scientific Reports     2020.12

  5. Mutational diversity in mutY deficient Helicobacter pylori and its effect on adaptation to the gastric environment Reviewed

    Ryo Kinoshita-Daitoku, Kotaro Kiga, Takahito Sanada, Yoshitoshi Ogura, Zhu Bo, Tamako Iida, Rui Yokomori, Eisuke Kuroda, Mototsugu Tanaka, Arpana Sood, Toshihiko Suzuki, Kenta Nakai, Tetsuya Hayashi, Hitomi Mimuro

    Biochemical and Biophysical Research Communications     2020.5

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Presentations 1

  1. sRNA regulates pathogenicity during persistent infection of Helicobacter Invited

    2020.2.21 

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    Language：English   Presentation type：Symposium, workshop panel (nominated)  

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KAKENHI (Grants-in-Aid for Scientific Research) 3

  1. 細菌のコントロール：Ωシステムを利用した次世代治療薬の創製

    Grant number：24K02686  2024.4 - 2027.3

    科学研究費助成事業  基盤研究(B)

    木下 遼, 氣駕 恒太朗

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    Authorship：Principal investigator 

    Grant amount：\18590000 （ Direct Cost: \14300000 、 Indirect Cost：\4290000 ）

    ヒトの腸内には約1000種類、100兆個以上の細菌が生息しており、腸内細菌叢と呼ばれている。腸内細菌叢の乱れに関連する疾患として偽膜性腸炎、難治性疾患である炎症性腸疾患などが報告されている。しかし、確立された治療法はまだない。そのため、生体内の細菌を特異的かつ持続的にコントロールする技術が求められている。申請者はこれまで細菌を特異的に殺菌できるシステムを探索してきた。その結果、RNA誘導型ヌクレアーゼが標的細菌を狙って特異的に殺菌できることを発見した。そこで本研究では、RNA誘導型ヌクレアーゼを細菌へ供給する遺伝子標的型治療薬の開発を企画した。

  2. Investigating Bacterial Dynamics and Developing New Antimicrobial Agents in Cambodia Using Innovative Genome Analysis Techniques

    Grant number：23KK0151  2023.9 - 2027.3

    Grants-in-Aid for Scientific Research  Fund for the Promotion of Joint International Research (International Collaborative Research)

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    Authorship：Coinvestigator(s) 

  3. Analysis of the mechanism of gastric inflammation induced by small RNAs of H. pylori.

    Grant number：20K16244  2020.4 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    Kinoshita-Daitoku Ryo

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    Authorship：Principal investigator 

    Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）

    Helicobacter pylori (H. pylori) infection is known to be involved in the development of gastrointestinal diseases such as gastritis, gastric ulcer, gastric cancer, and MALT lymphoma. In this study, we found that sRNA-X and sRNA-Z possessed by H. pylori are involved in the development of gastritis. Furthermore, we found that the expression levels of sRNA-X correlated with clinical findings based on the results of experiments using clinical isolates of H. pylori. These results indicate that the pathogenic sRNAs possessed by H. pylori contribute to gastritis caused by H. pylori.

To the head of KAKENHI (Grants-in-Aid for Scientific Research).▲