Updated on 2023/04/28


Graduate School of Pharmaceutical Sciences Department of Basic Medicinal Sciences Bioscience Assistant Professor
Graduate School
Graduate School of Pharmaceutical Sciences
Assistant Professor

Degree 1

  1. 博士(理学) ( 2018.3   大阪大学 ) 

Committee Memberships 1

  1. 日本神経科学学会   将来計画委員  


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    Committee type:Academic society


Papers 5

  1. Modeling the marmoset brain using embryonic stem cell-derived cerebral assembloids

    Kodera T., Takeuchi R.F., Takahashi S., Suzuki K., Kassai H., Aiba A., Shiozawa S., Okano H., Osakada F.

    Biochemical and Biophysical Research Communications   Vol. 657   page: 119 - 127   2023.5

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    Language:English   Publisher:Biochemical and Biophysical Research Communications  

    Studying the non-human primate (NHP) brain is required for the translation of rodent research to humans, but remains a challenge for molecular, cellular, and circuit-level analyses in the NHP brain due to the lack of in vitro NHP brain system. Here, we report an in vitro NHP cerebral model using marmoset (Callithrix jacchus) embryonic stem cell-derived cerebral assembloids (CAs) that recapitulate inhibitory neuron migration and cortical network activity. Cortical organoids (COs) and ganglionic eminence organoids (GEOs) were induced from cjESCs and fused to generate CAs. GEO cells expressing the inhibitory neuron marker LHX6 migrated toward the cortical side of CAs. COs developed their spontaneous neural activity from a synchronized pattern to an unsynchronized pattern as COs matured. CAs containing excitatory and inhibitory neurons showed mature neural activity with an unsynchronized pattern. The CAs represent a powerful in vitro model for studying excitatory and inhibitory neuron interactions, cortical dynamics, and their dysfunction. The marmoset assembloid system will provide an in vitro platform for the NHP neurobiology and facilitate translation into humans in neuroscience research, regenerative medicine, and drug discovery.

    DOI: 10.1016/j.bbrc.2023.03.019



  2. Processing of visual statistics of naturalistic videos in macaque visual areas V1 and V4. Reviewed International journal

    Hatanaka G, Inagaki M, Takeuchi RF, Nishimoto S, Ikezoe K, Fujita I

    Brain structure & function   Vol. 227 ( 4 ) page: 1385 - 1403   2022.5

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    DOI: 10.1007/s00429-022-02468-z


  3. Monosynaptic rabies virus tracing from projection-targeted single neurons Reviewed International journal

      Vol. 178   page: 20 - 32   2022.5

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  4. Temporally multiplexed dual-plane imaging of neural activity with four-dimensional precision Reviewed International journal

    Onda Masanari, Takeuchi Ryosuke F., Isobe Keisuke, Suzuki Toshiaki, Masaki Yuji, Morimoto Nao, Osakada Fumitaka

    NEUROSCIENCE RESEARCH   Vol. 171   page: 9 - 18   2021.10

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    Language:Japanese   Publisher:Neuroscience Research  

    Spatiotemporal patterns of neural activity generate brain functions, such as perception, memory, and behavior. Four-dimensional (4-D: x, y, z, t) analyses of such neural activity will facilitate understanding of brain functions. However, conventional two-photon microscope systems observe single-plane brain tissue alone at a time with cellular resolution. It faces a trade-off between the spatial resolution in the x-, y-, and z-axes and the temporal resolution by a limited point-by-point scan speed. To overcome this trade-off in 4-D imaging, we developed a holographic two-photon microscope for dual-plane imaging. A spatial light modulator (SLM) provided an additional focal plane at a different depth. Temporal multiplexing of split lasers with an optical chopper allowed fast imaging of two different focal planes. We simultaneously recorded the activities of neurons on layers 2/3 and 5 of the cerebral cortex in awake mice in vivo. The present study demonstrated the proof-of-concept of dual-plane two-photon imaging of neural circuits by using the temporally multiplexed SLM-based microscope. The temporally multiplexed holographic microscope, combined with in vivo labeling with genetically encoded probes, enabled 4-D imaging and analysis of neural activities at cellular resolution and physiological timescales. Large-scale 4-D imaging and analysis will facilitate studies of not only the nervous system but also of various biological systems.

    DOI: 10.1016/j.neures.2021.02.001

    Web of Science



  5. Cell type- and layer-specific convergence in core and shell neurons of the dorsal lateral geniculate nucleus Reviewed International journal

    Okigawa S., Yamaguchi M., Ito K.N., Takeuchi R.F., Morimoto N., Osakada F.

    Journal of Comparative Neurology   Vol. 529 ( 8 ) page: 2099 - 2124   2021.6

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    Language:Japanese   Publisher:Journal of Comparative Neurology  

    Over 40 distinct types of retinal ganglion cells (RGCs) generate parallel processing pathways in the visual system. In mice, two subdivisions of the dorsal lateral geniculate nucleus (dLGN), the core and the shell, organize distinct parallel channels to transmit visual information from the retina to the primary visual cortex (V1). To investigate how the dLGN core and shell differentially integrate visual information and other modalities, we mapped synaptic input sources to each dLGN subdivision at the cell-type level with G-deleted rabies viral vectors. The monosynaptic circuit tracing revealed that dLGN core neurons received inputs from alpha-RGCs, Layer 6 neurons of the V1, the superficial and intermediate layers of the superior colliculus (SC), the internal ventral LGN, the lower layer of the external ventral LGN (vLGNe), the intergeniculate leaf, the thalamic reticular nucleus (TRN), and the pretectal nucleus (PT). Conversely, shell neurons received inputs from alpha-RGCs and direction-selective ganglion cells of the retina, Layer 6 neurons of the V1, the superficial layer of the SC, the superficial and lower layers of the vLGNe, the TRN, the PT, and the parabigeminal nucleus. The present study provides anatomical evidence of the cell type- and layer-specific convergence in dLGN core and shell neurons. These findings suggest that dLGN core neurons integrate and process more multimodal information along with visual information than shell neurons and that LGN core and shell neurons integrate different types of information, send their own convergent information to discrete populations of the V1, and differentially contribute to visual perception and behavior.

    DOI: 10.1002/cne.25075



KAKENHI (Grants-in-Aid for Scientific Research) 2

  1. 広域機能イメージングと投影光遺伝学による大域的神経活動の因果的分析

    Grant number:22K15620  2022.4 - 2025.3

    科学研究費助成事業  若手研究

    竹内 遼介

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    Authorship:Principal investigator 

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    申請者は,本研究で構築する光学顕微鏡により,課題を遂行中のマウスにおいて,①大域的神経活動パターンの観察・同定,②大域的 / 局所的神経活動操作,③ 活動操作によって起きた行動の観察・定量

  2. A neural basis of the visuomotor integration with multi-scale functional imaging analysis

    Grant number:20K16464  2020.4 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    Takeuchi Ryosuke

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    Authorship:Principal investigator 

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    Using wide-field calcium imaging from mice with pan-excitatory neuron expression of GCaMP, we found that dorsal visual cortical areas and RSP significantly responded to visuomotor mismatches between visual flow feedback and mouse movement. Predictability of the visuomotor mismatch based on prior experiences significantly affected neural responses and the information flow in the posterior medial areas. Thus, we conclude that prediction error signals were hierarchically encoded and propagated across posterior cortical areas.