Updated on 2023/09/27

写真a

 
AKIYAMA Hirokazu
 
Organization
Graduate School of Engineering Biomolecular Engineering 2 Assistant Professor
Graduate School
Graduate School of Engineering
Undergraduate School
School of Engineering Chemistry and Biotechnology
Title
Assistant Professor
Contact information
メールアドレス
External link

Degree 1

  1. Doctor of Engineering ( 2011.3   Kyushu University ) 

Research Interests 9

  1. Bioprocess

  2. Stem Cells

  3. Regenerative Medicine

  4. Design of Experiments

  5. Cardiomyocyte

  6. Bioinformatics

  7. Bioengineering

  8. Tissue Engineering

  9. Organoids

Research Areas 1

  1. Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering

Current Research Project and SDGs 2

  1. Development of Stem Cell Differentiation Technologies for Regenerative Medicine and Drug Discovery

  2. Development of Cell Manufacturing Process for Regenerative Medicine

Research History 6

  1. Nagoya University   Assistant Professor

    2021.5

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    Country:Japan

  2. Revorf Co., Ltd.   Senior Scientist

    2020.5 - 2021.4

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    Country:Japan

  3. Kaneka Corporation   Assistant Manager

    2015.4 - 2020.4

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    Country:Japan

  4. University of Toronto   Institute of Biomaterials & Biomedical Engineering   Visiting Scientist

    2016.5 - 2018.4

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    Country:Canada

  5. Kaneka Corporation   Scientist

    2011.4 - 2015.3

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    Country:Japan

  6. Japan Society for Promotion of Science   Research Fellowship for Young Scientists (DC2)

    2009.4 - 2011.3

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    Country:Japan

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Education 3

  1. Kyushu University   Graduate School of Engineering   Chemical Systems and Engineering (Doctoral Course)

    2008.4 - 2011.3

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    Country: Japan

  2. Kyushu University   Graduate School of Engineering   Chemical Systems and Engineering (Master's Course)

    2007.4 - 2008.3

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    Country: Japan

  3. Kyushu University   Faculty of Engineering   Department of Materials Science and Engineering

    2003.4 - 2007.3

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    Country: Japan

Professional Memberships 4

  1. The Society of Chemical Engineers, Japan

  2. The Society for Biotechnology, Japan

  3. The Japanese Society for Regenerative Medicine

  4. Japanese Association for Animal Cell Technology

 

Papers 14

  1. Electrical pulse stimulation-induced tetanic exercise simulation increases the secretion of extracellular vesicles from C2C12 myotubes

    Murata Akari, Akiyama Hirokazu, Honda Hiroyuki, Shimizu Kazunori

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 672   page: 177 - 184   2023.9

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    Language:English   Publisher:Biochemical and Biophysical Research Communications  

    Extracellular vesicles (EVs) released into the blood during exercise mediate its whole-body health effects. The differentiation of EVs released by skeletal muscle cells in vivo from those released by other cells is challenging, therefore, it is unclear whether exercise increases the number of EVs secreted by skeletal muscle cells. In this study, we investigated whether exercise affects the quantity of EVs released from skeletal muscle cells using in vitro exercise models. C2C12 myotubes were cultured on a gel layer with 1 or 30 Hz electrical pulse stimulation (EPS) to induce contractions as an artificial simulating exercise. We found that tetanic contraction induced by 30 Hz EPS increased the number of secreted EVs. MicroRNA (miRNA)-seq analysis revealed that 30 Hz EPS altered the miRNA in the secreted EVs. Furthermore, expression analysis of genes related to the biogenesis and transport of EVs revealed that the expression of ALG-2 interacting protein X (Alix) was increased in response to 30 Hz EPS, and the peak value of intracellular Ca2+ in myotubes at 30 Hz EPS was higher than that at 1 Hz, indicating that the increase in intracellular Ca2+ concentration may be related to the increased secretion of EVs in response to 30 Hz EPS.

    DOI: 10.1016/j.bbrc.2023.06.054

    Web of Science

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    PubMed

  2. Alignment of Skeletal Muscle Cells Facilitates Acetylcholine Receptor Clustering and Neuromuscular Junction Formation with Co-Cultured Human iPSC-Derived Motor Neurons Reviewed

    Kazunori Shimizu, Haruo Kassai, Yuhei Kamei, Kazuki Yamamoto, Takunori Nagashima, Tadayoshi Maekawa, Hirokazu Akiyama, Hiroyuki Honda

    Cells   Vol. 11 ( 23 ) page: 3760 - 3760   2022.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MDPI AG  

    In vitro neuromuscular junction (NMJ) models are powerful tools for studying neuromuscular disorders. Although linearly patterned culture surfaces have been reported to be useful for the formation of in vitro NMJ models using mouse motor neuron (MNs) and skeletal muscle (SkM) myotubes, it is unclear how the linearly patterned culture surface increases acetylcholine receptor (AChR) clustering, one of the steps in the process of NMJ formation, and whether this increases the in vitro NMJ formation efficiency of co-cultured human MNs and SkM myotubes. In this study, we investigated the effects of a linearly patterned culture surface on AChR clustering in myotubes and examined the possible mechanism of the increase in AChR clustering using gene expression analysis, as well as the effects of the patterned surface on the efficiency of NMJ formation between co-cultured human SkM myotubes and human iPSC-derived MNs. Our results suggest that better differentiation of myotubes on the patterned surface, compared to the flat surface, induced gene expression of integrin α7 and AChR ε-subunit, thereby increasing AChR clustering. Furthermore, we found that the number of NMJs between human SkM cells and MNs increased upon co-culture on the linearly patterned surface, suggesting the usefulness of the patterned surface for creating in vitro human NMJ models.

    DOI: 10.3390/cells11233760

    Web of Science

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  3. Detection of protease digestion site using fluorescence labeled peptide array and construction of machine-learning prediction model Reviewed

    Mizutani Ryota, Mori Yoko, Ogawa Shodai, Tazoe Kaho, Akiyama Hirokazu, Shimizu Kazunori, Honda Hiroyuki

    Seibutsu-kogaku Kaishi   Vol. 100 ( 10 ) page: 528 - 540   2022.10

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:The Society for Biotechnology, Japan  

    <p>For the prediction of protease digestion site, 1990 kinds of tetramer peptide were designed as a library, of which N terminal end was fluorescence labelled. Decrease of fluorescence intensity of each peptide was quantitatively determined and used as a training data for Random Forest (RF) modeling. All of dipeptides bond as a protease substrate were included in 1990 tetramer peptides. As an explanatory variable for model construction, 532 parameters were prepared and those were included not only appearance of amino acid residue or dipeptides but also positioning parameters (PP) or global parameters (GP) to explain the peptide property. Trypsin for biochemistry grade was used for hydrolysis. After digesting at pH 8, the histogram of digestion ratio was appeared that tetramers with arginine residue (R) and lysine residue (K) could be expectedly digested at higher ratio compared with tetramers without R or K. Constructed pH 8-RF model showed 77 % of prediction accuracy. The explanatory parameters with top 10 higher importance in pH 8-RF model were both of appearance of K and 9 GPs. GPs were including 4 isoelectric parameters and 1 polarity parameter, of which K or R residue have relatively large value. Digestion site of α-lactalbumin was determined using pH 8-RF model and the protein was actually digested by trypsin. When the hydrolysate was analyzed by LC-MS/MS, 42 peptides were identified. Fourteen sites among 19 predicted digestion sites were coincided with each other. Many peptide fragments digested at 69Y-70G site or 50F-51H site was detected and it was strongly suggested to be digested by chymotrypsin as a contaminated protease. These sites were fairly predicted by constructed RF model. In addition, trypsin digestion at pH 5 was carried out to investigate the effect of pH decrease on trypsin digestion. The prediction accuracy of pH 5-RF model was only 59 %. The parameters with top 10 higher importance in pH 5-RF model were including 4 GPs which was the same with 4 isoelectric parameter in pH 8 model. Twenty digestion sites of α-lactalbumin were predicted from pH 5-RF model. α-lactalbumin was digested at pH 5 by trypsin, and 64 peptides were identified. The predicted sites were compared with identified fragments at the region from 48T to 77K in which a lot of fragments were detected. It was concluded that constructed model could fairly predict some of newly identified sites and could roughly grasp slight modification of digestion site by pH change. The proposed methodology, which contained 1990 kinds of tetramer peptides as a library, 532 explanatory parameters and RF model, was effective for prediction of protease digestion site.</p>

    DOI: 10.34565/seibutsukogaku.100.10_528

    DOI: 10.34565/seibutsukogaku.100.10_528

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    CiNii Research

  4. Screening of anti‐atrophic peptides by using photo‐cleavable peptide array and 96‐well scale contractile human skeletal muscle atrophy models Reviewed

    Kazuki Yamamoto, Saki Ohsumi, Takunori Nagashima, Hirokazu Akiyama, Hiroyuki Honda, Kazunori Shimizu

    Biotechnology and Bioengineering   Vol. 119 ( 8 ) page: 2196 - 2205   2022.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/bit.28125

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/bit.28125

  5. Embryoid Body-Explant Outgrowth Cultivation from Induced Pluripotent Stem Cells in an Automated Closed Platform Reviewed

    Hiroshi Tone, Saeko Yoshioka, Hirokazu Akiyama, Akira Nishimura, Masaki Ichimura, Masaru Nakatani, Tohru Kiyono, Masashi Toyoda, Masatoshi Watanabe, Akihiro Umezawa

    BioMed Research International   Vol. 2016   page: 7098987   2016

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1155/2016/7098987

    DOI: 10.1155/2016/7098987

  6. Comparison of manual and automated cultures of bone marrow stromal cells for bone tissue engineering Reviewed

    Hirokazu Akiyama, Asako Kobayashi, Masaki Ichimura, Hiroshi Tone, Masaru Nakatani, Minoru Inoue, Arinobu Tojo, Hideaki Kagami

    Journal of Bioscience and Bioengineering   Vol. 120 ( 5 ) page: 570 - 576   2015.11

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbiosc.2015.03.011

    DOI: 10.1016/j.jbiosc.2015.03.011

  7. Effects of B-cell lymphoma 2 gene transfer to myoblast cells on skeletal muscle tissue formation using magnetic force-based tissue engineering Reviewed

    Masanori Sato, Akira Ito, Hirokazu Akiyama, Yoshinori Kawabe, Masamichi Kamihira

    Tissue Engineering Part A   Vol. 19 ( 1-2 ) page: 307 - 315   2013.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1089/ten.TEA.2011.0728

    DOI: 10.1089/ten.TEA.2011.0728

  8. Construction of cardiac tissue rings using a magnetic tissue fabrication technique Reviewed

    Hirokazu Akiyama, Akira Ito, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira

    International Journal of Molecular Sciences   Vol. 11 ( 8 ) page: 2910 - 2920   2010.8

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/ijms11082910

    DOI: 10.3390/ijms11082910

  9. Cell-patterning using poly (ethylene glycol)-modified magnetite nanoparticles Reviewed

    Hirokazu Akiyama, Akira Ito, Masanori Sato, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Biomedical Materials Research Part A   Vol. 92 ( 3 ) page: 1123 - 1130   2010.5

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/jbm.a.32313

    DOI: 10.1002/jbm.a.32313

  10. Genetically engineered angiogenic cell sheets using magnetic force-based gene delivery and tissue fabrication techniques Reviewed

    Hirokazu Akiyama, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

    Biomaterials   Vol. 31 ( 6 ) page: 1251 - 1259   2010.2

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.biomaterials.2009.11.017

    DOI: 10.1016/j.biomaterials.2009.11.017

  11. Fabrication of angiogenic gene-modified myoblast cell sheets using magnetic tissue engineering techniques.

    Hirokazu Akiyama, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

    Proceedings of the World Congress on Engineering and Computer Science   Vol. 2   page: 806 - 810   2010

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    Authorship:Lead author   Language:English   Publishing type:Research paper (international conference proceedings)  

  12. Magnetic cell-patterning for tissue engineering

    Hirokazu Akiyama, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

    Animal Cell Technology: Basic & Applied Aspects (Proceedings of JAACT2008)   Vol. 16   page: 165 - 170   2010

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    Authorship:Lead author   Language:English   Publishing type:Research paper (international conference proceedings)  

    DOI: 10.1007/978-90-481-3892-0_27

  13. Fabrication of complex three-dimensional tissue architectures using a magnetic force-based cell patterning technique Reviewed

    Hirokazu Akiyama, Akira Ito, Yoshinori Kawabe, Masamichi Kamihira

    Biomedical Microdevices   Vol. 11 ( 4 ) page: 713 - 721   2009.8

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10544-009-9284-x

    DOI: 10.1007/s10544-009-9284-x

  14. Magnetic force-based cell patterning using Arg-Gly-Asp (RGD) peptide-conjugated magnetite cationic liposomes Reviewed

    Akira Ito, Hirokazu Akiyama, Yoshinori Kawabe, Masamichi Kamihira

    Journal of Bioscience and Bioengineering   Vol. 104 ( 4 ) page: 288 - 293   2007.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1263/jbb.104.288

    DOI: 10.1263/jbb.104.288

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Books 2

  1. 三次元ティッシュエンジニア リング~細胞の培養・操作・組織化から品質管理、脱細胞化まで~・間葉系幹細胞向け自動培養装置(4章2節)

    刀禰宏、秋山裕和( Role: Joint author)

    エヌ・ティー・エス  2015 

  2. ≪最 新≫動物細胞培養の手法と細胞死・増殖不良・細胞変異を防止する技術・閉鎖型自動培養装置による間葉系幹細胞の培養(2章4節12項)

    秋山裕和、櫻井裕士( Role: Joint author)

    技術情報協会  2014 

Research Project for Joint Research, Competitive Funding, etc. 7

  1. Generation of Heart Wall Organoids and thier Pharmaceutical Application

    2023.8 - 2024.7

    Foundation of Public Interest of Tatematsu  The 32st, Research grant FY 2023 

    Hirokazu Akiyama

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\1500000

  2. 多能性幹細胞の共分化誘導に基づく細胞機能最大化培養プロセスの開発

    2023.5 - 2024.4

    一般財団法人 東海産業技術振興財団  第35回 助成研究(研究育成型) 

    秋山裕和

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    Grant type:Competitive

    Grant amount:\900000

  3. 再生医療応用を目指したiPS細胞の共分化誘導プロセスの開発

    2023.4 - 2024.3

    一般財団法人伊藤忠兵衛基金  2023年(令和5年)度 学術研究助成金 

    秋山裕和

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\500000

  4. Development of Cardiomyocyte/Epicardial Cell Co-differentiation Process from Pluripotent Stem Cells for Next-generation Regenerative Medicine

    2022.12 - 2023.11

    The Public Foundation of Chubu Science and Technology Center  Academic / Mirai Grant 

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\300000

  5. Development of Simultaneous Generation Process of Cardiac Muscle Tissue Cells from Pluripotent Stem Cells

    2022.5 - 2023.4

    The Naito Science & Engineering Foundation  The Naito Research Grant 

    Hirokazu Akiyama

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\1000000

  6. 臍帯血造血幹細胞の培養プロセスの開発

    2016.5 - 2018.4

    カネカ 技術振興基金(海外留学) 

    秋山裕和

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    Authorship:Principal investigator  Grant type:Other

    Grant amount:\4000000

  7. Fabrication of angiogenic gene-modified myoblast cell sheets using magnetic tissue engineering techniques

    2010.10

    Kato Memorial Bioscience Foundation  The Kato Memorial Bioscience Grant for International Research Fellowship 

    Hirokazu Akiyama

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\200000

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KAKENHI (Grants-in-Aid for Scientific Research) 4

  1. マイクロデバイスを用いた微小環境精密制御による筋オルガノイド機能発現と病態解析

    Grant number:23K17474  2023.6 - 2026.3

    科学研究費助成事業  挑戦的研究(開拓)

    清水 一憲, 秋山 裕和

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    Authorship:Coinvestigator(s) 

    オルガノイド(幹細胞を用いて作製した生体器官に類似した培養立体組織)は、平面培養細胞や動物実験に代わる技術として、病気の研究や薬の開発に利用することが期待されています。本研究では、マイクロデバイス技術を活用してオルガノイド機能発現を誘導し、それを用いて疾患解析を行うことを目的としています。

  2. Development of Co-differentiation Process of Pluripotent Stem Cells for Next-generation Regenerative Medicine

    2023.4 - 2026.3

    Japan Society for the Promotion of Science  Grant-in-Aid for Scientific Research (KAKENHI)  Grant-in-Aid for Scientific Research (C)

    Hirokazu Akiyama, Kazunori Shimizu

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

  3. Establishment of efficient exploring method for non-inferior bioactive peptides from edible proteins

    Grant number:22H00273  2022.4 - 2026.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)  Grant-in-Aid for Scientific Research (A)

    Hiroyuki Honda, Hirokazu Akiyama, Kazunori Shimizu

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    Authorship:Coinvestigator(s)  Grant type:Competitive

  4. 磁性ナノ粒子を用いた血管配置及び誘導による骨格筋組織再生

    Grant number:09J02784  2009.4 - 2011.3

    日本学術振興会  科学研究費補助金  特別研究員奨励費(DC2)

    秋山裕和

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\1400000

 

Teaching Experience (On-campus) 8

  1. 化学生命工学実験2

    2023

  2. Chemistry/Biochemistry Laboratory III

    2022

  3. Chemistry/Biochemistry Laboratory II

    2022

  4. 卒業研究

    2022

  5. 化学生命工学実験3

    2022

  6. Chemistry/Biochemistry Laboratory III

    2021

  7. Chemistry/Biochemistry Laboratory II

    2021

  8. 卒業研究

    2021

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