Updated on 2022/04/15

写真a

 
SUGITA Ryohei
 
Organization
Radioisotope Research Center Investigators directory Lecturer
Title
Lecturer
External link

Degree 2

  1. 博士(農学) ( 2014.4   東京大学 ) 

  2. 修士(農学) ( 2007.3   東京大学 ) 

Research Interests 8

  1. 養分

  2. 植物栄養

  3. 光合成

  4. 元素動態

  5. リアルタイムイメージング

  6. イメージング装置

  7. アイソトープイメージング

  8. アイソトープ

Research Areas 2

  1. Environmental Science/Agriculture Science / Agricultural environmental engineering and agricultural information engineering

  2. Life Science / Plant nutrition and soil science

Current Research Project and SDGs 2

  1. 耐塩性植物の創出

  2. 植物体内イオン動態の解明

Research History 4

  1. Nagoya University   Radioisotope Research Center   Lecturer

    2021.4

  2. The University of Tokyo   Graduate School of Agricultural and Life Sciences   Assistant Professor

    2019.4 - 2021.3

  3. The University of Tokyo   Graduate School of Agricultural and Life Sciences   Designated assistant professor

    2016.8 - 2019.3

  4. The University of Tokyo   Graduate School of Agricultural and Life Sciences

    2012.4 - 2016.7

Professional Memberships 2

  1. アイソトープ協会

  2. 日本土壌肥料学会

Committee Memberships 1

  1. Applied Sciences   Editorial Board of Applied Sciences  

    2020.10   

Awards 4

  1. IWPMB EXCELLENT POSTER AWARD

    2016.6  

  2. RADIOISOTOPES誌論文奨励賞

    2015.7  

  3. 2014年度エヌエフ基金研究開発奨励賞

    2014.4  

  4. 第14回東京大学生命科学シンポジウム 優秀ポスター賞

    2014.4  

 

Papers 23

  1. Visualization of phosphorus re-translocation and phosphate transporter expression profiles in a shortened annual cycle system of poplar.

    Kurita Y, Kanno S, Sugita R, Hirose A, Ohnishi M, Tezuka A, Deguchi A, Ishizaki K, Fukaki H, Baba K, Nagano AJ, Tanoi K, Nakanishi TM, Mimura T

    Plant, cell & environment     2022.3

     More details

    Language:English  

    DOI: 10.1111/pce.14319

    PubMed

  2. Autoradiography system with phosphor powder (ZnS:Ag) for imaging radioisotope dynamics in a living plant

    Kurita Keisuke, Sakai Takuro, Suzui Nobuo, Yin Yong-Gen, Sugita Ryohei, Kobayashi Natsuko I, Tanoi Keitaro, Kawachi Naoki

    JAPANESE JOURNAL OF APPLIED PHYSICS   Vol. 60 ( 11 )   2021.11

     More details

    Language:Japanese   Publisher:Japanese Journal of Applied Physics  

    Radioisotope tracer imaging is useful for studying plant physiological phenomena. In this study, we developed an autoradiography system with phosphor powder (ZnS:Ag), "Live-autoradiography", for imaging radioisotope dynamics in a living plant. This system visualizes the element migration and accumulation in intact plants continuously under a light environment. An imaging test was performed on point sources of 137Cs, with radioactivity of 10-100 kBq of being observed; this indicates satisfactory system linearity between the image intensity and the radioactivity of 137Cs. Moreover, dynamics imaging of 137Cs was performed on an intact soybean plant for four days. The serial images indicated 137Cs accumulation in the node, vein, and growing point of the plant. The developed system can be used for studying plant physiological phenomena and can be employed for the quantitative measurement of radionuclides.

    DOI: 10.35848/1347-4065/ac2b50

    Web of Science

    Scopus

  3. ATP binding cassette proteins ABCG37 and ABCG33 function as potassium-independent cesium uptake carriers in Arabidopsis roots. International journal

    Mohammad Arif Ashraf, Takashi Akihiro, Keita Ito, Sayaka Kumagai, Ryohei Sugita, Keitaro Tanoi, Abidur Rahman

    Molecular plant   Vol. 14 ( 4 ) page: 664 - 678   2021.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Radiocesium accumulated in the soil by nuclear accidents is a major environmental concern. The transport process of cesium (Cs+) is tightly linked to the indispensable plant nutrient potassium (K+) as they both belong to the group I alkali metals with similar chemical properties. Most of the transporters that had been characterized to date as Cs+ transporters are directly or indirectly linked to K+. Using a combinatorial approach of physiology, genetics, cell biology, and root uptake assay, here we identified two ATP-binding cassette (ABC) proteins, ABCG37 and ABCG33, as facilitators of Cs+ influx. A gain-of-function mutant of ABCG37 (abcg37-1) showed increased sensitivity to Cs+-induced root growth inhibition, while the double knockout mutant of ABCG33 and ABCG37 (abcg33-1abcg37-2) showed resistance, whereas the single loss-of-function mutants of ABCG33 and ABCG37 did not show any alteration in Cs+ response. In planta short-term radioactive Cs+-uptake assay along with growth and uptake assays in a heterologous system confirmed ABCG33 and ABCG37 as Cs+-uptake carriers. Potassium response and content were unaffected in the double-mutant background and yeast cells lacking potassium-uptake carriers transformed with ABCG33 and ABCG37 failed to grow in the absence of K+, confirming that Cs+ uptake by ABCG33 and ABCG37 is independent of K+. Collectively, this work identified two ABC proteins as new Cs+-influx carriers that act redundantly and independent of the K+-uptake pathway.

    DOI: 10.1016/j.molp.2021.02.002

    PubMed

  4. リアルタイムRIイメージングを用いた光の変化がイネの元素動態に与える影響の解析

    杉田 亮平, 小林 奈通子, 廣瀬 農, 岩田 錬, 鈴木 寿, 田野井 慶太朗, 中西 友子

    アイソトープ・放射線研究発表会   Vol. 1 ( 0 ) page: 36   2021

     More details

    Language:Japanese   Publisher:公益社団法人 日本アイソトープ協会  

    <p>リアルタイムRIイメージングを用いた光の変化がイネの元素動態に与える影響の解析</p>

    DOI: 10.50955/happyokai.1.0_36

    CiNii Research

  5. Short-Term Magnesium Deficiency Triggers Nutrient Retranslocation in Arabidopsis thaliana. International journal

    Takaaki Ogura, Natsuko I Kobayashi, Christian Hermans, Yasunori Ichihashi, Arisa Shibata, Ken Shirasu, Naohiro Aoki, Ryohei Sugita, Takahiro Ogawa, Hisashi Suzuki, Ren Iwata, Tomoko M Nakanishi, Keitaro Tanoi

    Frontiers in plant science   Vol. 11   page: 563 - 563   2020

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Magnesium (Mg) is essential for many biological processes in plant cells, and its deficiency causes yield reduction in crop systems. Low Mg status reportedly affects photosynthesis, sucrose partitioning and biomass allocation. However, earlier physiological responses to Mg deficiency are scarcely described. Here, we report that Mg deficiency in Arabidopsis thaliana first modified the mineral profile in mature leaves within 1 or 2 days, then affected sucrose partitioning after 4 days, and net photosynthesis and biomass production after 6 days. The short-term Mg deficiency reduced the contents of phosphorus (P), potassium, manganese, zinc and molybdenum in mature but not in expanding (young) leaves. While P content decreased in mature leaves, P transport from roots to mature leaves was not affected, indicating that Mg deficiency triggered retranslocation of the mineral nutrients from mature leaves. A global transcriptome analysis revealed that Mg deficiency triggered the expression of genes involved in defence response in young leaves.

    DOI: 10.3389/fpls.2020.00563

    PubMed

  6. Ⅰ-1 植物の長距離輸送を調べるためのβ-線放出核種の非破壊イメージング手法(Ⅰ 植物の元素イメージング 2020年度岡山大会)

    田野井 慶太朗, 杉田 亮平, 中西 友子

    日本土壌肥料学会講演要旨集   Vol. 66 ( 0 ) page: 149 - 149   2020

     More details

    Language:Japanese   Publisher:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.66.0_149_1

  7. Plastic Scintillators Enable Live Imaging of <sup>32</sup>P-labelled Phosphorus Movement in Large Plants

    Kohei Sugahara, Ryohei Sugita, Natsuko I. Kobayashi, Atsushi Hirose, Tomoko M. Nakanishi, Etsuko Furuta, Masaaki Sensui, Keitaro Tanoi

    RADIOISOTOPES   Vol. 68 ( 3 ) page: 73 - 82   2019.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Japan Radioisotope Association  

    <p>We updated our imaging system, namely real-time radioisotope imaging system (RRIS) to observe <sup>32</sup>P movement in large plants using a large size scintillator. In the previous version of RRIS, the view was limited by the size of a CsI (Tl) scintillator deposited on a fiber optic plate (FOS). However, owing to the high price and difficulty in handling of FOS, the view area was limited to 100×200 mm. In this study, we evaluated 5 large and low-cost plastic scintillators and showed that plastic scintillators can be used in RRIS for the quantitative analysis of <sup>32</sup>P. We also evaluated the resolution of the imaging system with plastic scintillator.</p>

    DOI: 10.3769/radioisotopes.68.73

    CiNii Books

  8. Plastic Scintillators Enable Live Imaging of <sup>32</sup>P-labelled Phosphorus Movement in Large Plants

    菅原康平, 杉田亮平, 小林奈通子, 廣瀬農, 中西友子, 古田悦子, 泉水征昭, 田野井慶太朗, 田野井慶太朗

    RADIOISOTOPES (Web)   Vol. 68 ( 3 )   2019

     More details

  9. Evaluation of plastic scintillators for live imaging of 14C-labeled photosynthate movement in plants

    Ryohei Sugita, Kohei Sugahara, Natsuko I. Kobayashi, Atsushi Hirose, Tomoko M. Nakanishi, Etsuko Furuta, Masaaki Sensui, Keitaro Tanoi

    Journal of Radioanalytical and Nuclear Chemistry   Vol. 318 ( 1 ) page: 579 - 584   2018.10

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10967-018-6102-z

    Other Link: http://link.springer.com/content/pdf/10.1007/s10967-018-6102-z.pdf

  10. Magnesium deficiency damages the youngest mature leaf in rice through tissue-specific iron toxicity

    Natsuko I. Kobayashi, Takaaki Ogura, Keiko Takagi, Ryohei Sugita, Hisashi Suzuki, Ren Iwata, Tomoko M. Nakanishi, Keitaro Tanoi

    Plant and Soil   Vol. 428 ( 1-2 ) page: 137 - 152   2018.7

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    Magnesium deficiency can cause starch accumulation, photosynthesis inhibition and senescence particularly in young mature leaves. This study was performed to identify the initial process leading to leaf senescence under Mg deficiency.Gene expression in the young leaf was analyzed at days 2, 4, 5 of Mg deficiency using microarray analysis, and several Fe responsive genes were identified. Therefore, the effect of lowering Fe supply on gene expression and oxidative stress under Mg deficiency was evaluated.Transcriptome analysis revealed that 7 of the 30 most upregulated genes and 11 of the 30 most downregulated genes were Fe-responsive. Particularly, the upregulation of OsFER2 and downregulation of OsMIR and OsIRO2 hinted at the induction of excess Fe stress under Mg deficiency. Both lowering of Fe concentration in Mg-free solutions and resupply of Mg without modifying Fe concentrations at day 4 rescued leaves from senescence by inhibiting oxidative stress and normalising the expression of Fe-responsive genes. Meanwhile, Fe content was equal between control, Mg-deficient and Mg-resupplied plants.Mg shortage can induce excess Fe stress, which in turn causes oxidative stress before inhibition of photosynthesis. It is proposed that Mg deficiency disrupts a mechanism for storing toxic Fe ions into the vacuole in the expanding young leaf cells.

    DOI: 10.1007/s11104-018-3658-x

    Web of Science

    Other Link: http://link.springer.com/content/pdf/10.1007/s11104-018-3658-x.pdf

  11. Visualization of 14CO2 gas fixation by plants. International journal

    Ryohei Sugita, Natsuko I Kobayashi, Keitaro Tanoi, Tomoko M Nakanishi

    Journal of radioanalytical and nuclear chemistry   Vol. 318 ( 1 ) page: 585 - 590   2018

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Using the real-time radioisotope imaging system (RRIS), we present the carbon dioxide gas fixation process of a soybean plant applying the 14C-labeled gas. When 14CO2 gas was supplied to the selected mature leaf, the fixed carbon, photosynthate, was transferred and accumulated to the younger leaves preferentially within 24 h. When 14CO2 gas was supplied to the younger leaves, fixed carbon was hardly moved. In the case of the pods, fixed 14CO2 gas in the leaf was preferentially transferred to the closest pod.

    DOI: 10.1007/s10967-018-6119-3

    PubMed

  12. P4-1-13 プラスチックシンチレータを利用したリアルタイムRIイメージングシステムによる植物中の元素動態解析(ポスター,4-1 植物の多量栄養素,2018年度神奈川大会)

    菅原 康平, 杉田 亮平, 小林 奈通子, 廣瀬 農, 中西 友子, 古田 悦子, 泉水 征昭, 田野井 慶太朗

    日本土壌肥料学会講演要旨集   Vol. 64 ( 0 ) page: 57 - 57   2018

     More details

    Language:Japanese   Publisher:一般社団法人 日本土壌肥料学会  

    DOI: 10.20710/dohikouen.64.0_57_1

  13. Visualization of how light changes affect ion movement in rice plants using a real-time radioisotope imaging system

    Ryohei Sugita, Natsuko I. Kobayashi, Atsushi. Hirose, Ren Iwata, Hisashi Suzuki, Keitaro Tanoi, Tomoko M. Nakanishi

    Journal of Radioanalytical and Nuclear Chemistry   Vol. 312 ( 3 ) page: 717 - 723   2017.6

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    We developed a real-time radioisotope imaging system (RRIS) technique that can nondestructively visualize the element absorption and transport process in plants, using not only positron emitters but also commercially available radioisotopes. In this study, we applied RRIS to analyze light effects on ion movement in rice plants. As tracers, Mg-28, P-32, and Ca-45 were used. During the first 5 h, dark/light cycle of 3/7 min were set up; the RRIS needs dark conditions during capturing. When the light was ceased 5 h after supplying each tracer, P-32 transport from root to shoot decreased immediately. In contrast, Mg-28 and Ca-45 transport did not change with light conditions. These results suggest that the P transport is dependent on water flow, whereas Mg and Ca transport are independent of water flow.

    DOI: 10.1007/s10967-017-5193-2

    Web of Science

    Other Link: http://link.springer.com/content/pdf/10.1007/s10967-017-5193-2.pdf

  14. Visualization of Uptake of Mineral Elements and the Dynamics of Photosynthates in Arabidopsis by a Newly Developed Real-Time Radioisotope Imaging System (RRIS).

    Ryohei Sugita, Natsuko I Kobayashi, Atsushi Hirose, Takayuki Saito, Ren Iwata, Keitaro Tanoi, Tomoko M Nakanishi

    Plant & cell physiology   Vol. 57 ( 4 ) page: 743 - 53   2016.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    Minerals and photosynthates are essential for many plant processes, but their imaging in live plants is difficult. We have developed a method for their live imaging in Arabidopsis using a real-time radioisotope imaging system. When each radioisotope,(22)Na,(28)Mg,(32)P-phosphate,(35)S-sulfate,(42)K,(45)Ca,(54)Mn and(137)Cs, was employed as an ion tracer, ion movement from root to shoot over 24 h was clearly observed. The movements of(22)Na,(42)K,(32)P,(35)S and(137)Cs were fast so that they spread to the tip of stems. In contrast, high accumulation of(28)Mg,(45)Ca and(54)Mn was found in the basal part of the main stem. Based on this time-course analysis, the velocity of ion movement in the main stem was calculated, and found to be fastest for S and K among the ions we tested in this study. Furthermore, application of a heat-girdling treatment allowed determination of individual ion movement via xylem flow alone, excluding phloem flow, within the main stem of 43-day-old Arabidopsis inflorescences. We also successfully developed a new system for visualizing photosynthates using labeled carbon dioxide,(14)CO2 Using this system, the switching of source/sink organs and phloem flow direction could be monitored in parts of whole shoots and over time. In roots,(14)C photosynthates accumulated intensively in the growing root tip area, 200-800 µm behind the meristem. These results show that this real-time radioisotope imaging system allows visualization of many nuclides over a long time-course and thus constitutes a powerful tool for the analysis of various physiological phenomena.

    DOI: 10.1093/pcp/pcw056

    PubMed

  15. Tracer experiment using 42K+ and 137Cs+ revealed the different transport rates of potassium and caesium within rice roots. International journal

    Natsuko I Kobayashi, Ryohei Sugita, Tatsuya Nobori, Keitaro Tanoi, Tomoko M Nakanishi

    Functional plant biology : FPB   Vol. 43 ( 2 ) page: 151 - 160   2016.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    The differences in the transport characteristics in planta between potassium (K+) and caesium (Cs+) was investigated using their radionuclides, 42K+ and 137Cs+. A tracer experiment using nutrient solutions supplemented with 42K and 137Cs revealed that the ratio of the root's K+ uptake rate to its Cs+ uptake rate was 7-11 times higher than the K+:Cs+ concentration ratio in the solution, and the number was varied depending on the K concentration in the solution and also on the growth condition. After entering through the root tissues, the 42K+:137Cs+ ratio in the shoots was 4.28 times higher than the value in the roots. However, the 42K+:137Cs+ ratio in each leaf did not differ significantly, indicating that the primary transport of K+ and Cs+ in the shoots are similarly regulated. In contrast, among the radionuclides stored in the roots over 4h, 30% of the 42K+ was exported from the roots over the following hour, whereas only 8% of 137Cs+ was exported. In addition, within the xylem, K+ was shown to travel slowly, whereas Cs+ passed quickly through the roots into the shoots. In conclusion, our study demonstrated very different transport patterns for the two ions in the root tissues.

    DOI: 10.1071/FP15245

    PubMed

  16. Investigation of radiocesium distribution in organs of wild boar grown in Iitate, Fukushima after the Fukushima Daiichi nuclear power plant accident

    Keitaro Tanoi, Kazuyuki Uchida, Chiyo Doi, Naoto Nihei, Atsushi Hirose, Natsuko I. Kobayashi, Ryohei Sugita, Tatsuya Nobori, Tomoko M. Nakanishi, Muneo Kanno, Ippei Wakabayashi, Miicha Ogawa, Yoichi Tao

    Journal of Radioanalytical and Nuclear Chemistry   Vol. 307 ( 1 ) page: 741 - 746   2016.1

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10967-015-4233-z

    Other Link: http://link.springer.com/article/10.1007/s10967-015-4233-z/fulltext.html

  17. 土壌粒子の微生物

    西山雅也, 西山雅也, 杉田亮平, 田野井孝子, 大塚重人, 妹尾啓史

    土と微生物   Vol. 69 ( 2 )   2015

     More details

  18. Effects of magnesium deficiency on magnesium uptake activity of rice root, evaluated using (28) Mg as a tracer

    Keitaro Tanoi, Natsuko I. Kobayashi, Takayuki Saito, Naoko Iwata, Risa Kamada, Ren Iwata, Hisashi Suzuki, Atsushi Hirose, Yoshimi Ohmae, Ryohei Sugita, Tomoko M. Nakanishi

    PLANT AND SOIL   Vol. 384 ( 1-2 ) page: 69 - 77   2014.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    The mechanisms underlying magnesium (Mg) uptake by plant roots remain to be fully elucidated. In particular, there is little information about the effects of Mg deficiency on Mg uptake activity. A Mg uptake kinetic study is essential for better understanding the Mg uptake system.We performed a Mg uptake tracer experiment in rice plants using (28) Mg.Mg uptake was mediated by high- and low-affinity transport systems. The K (m) value of the high-affinity transport system was approximately 70 mu M under Mg-deficient conditions. The Mg uptake activity was promoted by Mg deficiency, which in turn fell to the basal level after 5- min of Mg resupply. The induced uptake rate was inhibited by ionophore treatment, suggesting that an energy-dependent uptake system is enhanced by Mg deficiency.The Mg uptake changes rapidly with Mg conditions in rice, as revealed by a (28) Mg tracer experiment. This technique is expected to be applicable for Mg uptake analyses, particularly in mutants or other lines.

    DOI: 10.1007/s11104-014-2197-3

    Web of Science

  19. Evaluation of in vivo detection properties of 22Na, 65Zn, 86Rb, 109Cd and 137Cs in plant tissues using real-time radioisotope imaging system. International journal

    Ryohei Sugita, Natsuko I Kobayashi, Atsushi Hirose, Keitaro Tanoi, Tomoko M Nakanishi

    Physics in medicine and biology   Vol. 59 ( 4 ) page: 837 - 51   2014.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    In plant research, radioisotope imaging provides useful information about physiological activities in various tissues and elemental transport between plant organs. To expand the usage of imaging techniques, a new system was developed to visualize beta particles, x-rays and gamma-rays emitted from plant bodies. This real-time radioisotope imaging system (RRIS) visualizes radioactivity after conversion into light with a CsI(Tl) scintillator plate. Herein, the RRIS detection properties of the gamma-ray emitters (22)Na, (65)Zn, (86)Rb, (109)Cd and (137)Cs were evaluated in comparison with those of radioluminography (RLG) using an imaging plate. The lower quantitative detection limit (Bq mm(-2)) during a 15 min period ranged from 0.1 to 4, depending on the nuclide, similar to that of RLG. When the quantitative ability to detect radiation from various Arabidopsis tissues was analyzed, the quantitative capability in silique and the thick internode tended to be low. In an EGS5 simulation, beta particles were the greatest contributors to RRIS imaging of (22)Na, (86)Rb and (137)Cs, and low-energy x-rays contributed significantly to (65)Zn and (109)Cd detection. Thus, both self-absorption and air space between the sample and scintillator surface could impair quantitative RRIS imaging. Despite these issues, RRIS is suggested for quantitative time-course measurements of radionuclide motion within plants.

    DOI: 10.1088/0031-9155/59/4/837

    PubMed

  20. Quantitative Analysis of 28Mg in Arabidopsis using Real-time Radioisotope Imaging System(RRIS)

    Ryohei SUGITA, Natsuko I. KOBAYASHI, Takayuki SAITO, Atsushi HIROSE, Ren IWATA, Keitaro TANOI, Tomoko M. NAKANISHI

    RADIOISOTOPES   Vol. 63 ( 5 ) page: 227 - 237   2014

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Japan Radioisotope Association  

    DOI: 10.3769/radioisotopes.63.227

    J-GLOBAL

  21. Nondestructive real-time radioisotope imaging system for visualizing 14C-labeled chemicals supplied as CO2 in plants using Arabidopsis thaliana

    Ryohei Sugita, Natsuko I. Kobayashi, Atsushi Hirose, Yoshimi Ohmae, Keitaro Tanoi, Tomoko M. Nakanishi

    Journal of Radioanalytical and Nuclear Chemistry   Vol. 298 ( 2 ) page: 1411 - 1416   2013.11

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10967-013-2462-6

    Other Link: http://link.springer.com/article/10.1007/s10967-013-2462-6/fulltext.html

  22. Carbon-14 labelled sucrose transportation in an Arabidopsis thaliana using an imaging plate and real time imaging system

    Yoshimi Ohmae, Atsushi Hirose, Ryohei Sugita, Keitaro Tanoi, Tomoko M. Nakanishi

    Journal of Radioanalytical and Nuclear Chemistry   Vol. 296 ( 1 ) page: 413 - 416   2013.4

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10967-012-2131-1

    Other Link: http://link.springer.com/article/10.1007/s10967-012-2131-1/fulltext.html

  23. Development of a 14C detectable real-time radioisotope imaging system for plants under intermittent light environment

    A. Hirose, M. Yamawaki, S. Kanno, S. Igarashi, R. Sugita, Y. Ohmae, K. Tanoi, T. M. Nakanishi

    Journal of Radioanalytical and Nuclear Chemistry   Vol. 296 ( 1 ) page: 417 - 422   2013.4

     More details

    Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10967-012-2130-2

    Other Link: http://link.springer.com/article/10.1007/s10967-012-2130-2/fulltext.html

▼display all

MISC 5

  1. Development of a live imaging system using radioactive tracers to study the dynamics of mineral elements in living plants

    Plant morphology   Vol. 33 ( 1 ) page: 9 - 13   2021

     More details

    Language:Japanese   Publisher:The Japanese Society of Plant Morphology  

    CiNii Books

  2. 植物体内の元素の動きを見る

    杉田亮平, 小林奈通子, 中西友子, 田野井慶太朗, 田野井慶太朗

    アグリバイオ   Vol. 3 ( 2 )   2019

     More details

  3. リアルタイムRIイメージングシステムを用いた植物根における元素輸送解析

    杉田亮平, 小林奈通子, 菅野里美, 廣瀬農, 田野井慶太朗, 中西友子

    アイソトープ・放射線研究発表会要旨集   Vol. 52nd   2015

     More details

  4. ミクロRIイメージングシステムによる根の<sup>33</sup>Pイメージング解析

    菅野里美, 杉田亮平, 廣瀬農, 田野井慶太朗, NUSSAUME Laurent, 三村徹郎, 中西友子

    アイソトープ・放射線研究発表会要旨集   Vol. 51st   2014

     More details

  5. 4-1-22 リアルタイムRIイメージングを用いた多核種の輸送動態解析(4-1 植物の多量栄養素)

    杉田 亮平, 斉藤 貴之, 小林 奈通子, 広瀬 農, 田野井 慶太朗, 中西 友子

    日本土壌肥料学会講演要旨集   ( 59 ) page: 61 - 61   2013.9

     More details

    Language:Japanese   Publisher:一般社団法人日本土壌肥料学会  

    CiNii Books

Presentations 5

  1. 放射線イメージングによる根の養分ダイナミクスの解明

    杉田亮平

    第63回日本植物生理学会  2022.3 

     More details

    Event date: 2022.3

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  2. 植物体内イオンの可視化に向けたRIイメージング装置の開発 Invited

    第67回応用物理学会春季学術講演会  2020.2 

  3. 放射性同位元素を利用した植物体内イオンのライブイメージング Invited

    11th NIBBバイオイメージングフォーラム  2017.2 

  4. 放射性トレーサーを用いた植物体内イオンの可視化技術の開発 Invited

    日本植物学会第84回大会  2020.9 

  5. RIライブイメージングを用いた植物体内イオンの可視化 Invited

    Optics & Photonics Japan  2019.12 

KAKENHI (Grants-in-Aid for Scientific Research) 13

  1. シロイヌナズナにおけるナトリウムイオン輸送体SOS1局在部位の同定

    2021.4 - 2022.3

    国立大学法人岡山大学資源植物科学研究所  共同研究課題 

      More details

    Authorship:Principal investigator 

  2. 超個体化における植物全身応答とその動態を暴き出す超個体イメージャーの構築

    Grant number:21H05152  2021

    学術変革領域研究(B)

      More details

    Authorship:Coinvestigator(s)  Grant type:Competitive

    Direct Cost: \4000000 )

  3. マルチモードイメージング情報を基盤とするITによる作物生産プロセスの熟成

    Grant number:20H00437  2020.4 - 2025.3

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    中西 友子, 二瓶 直登, 岩田 錬, 杉田 亮平, 青木 直大, 廣瀬 農, 森野 佳生, 二瓶 直登, 岩田 錬, 杉田 亮平, 青木 直大, 廣瀬 農, 森野 佳生

      More details

    Authorship:Coinvestigator(s) 

    本研究では、最適な施肥による収量の最大化を目的として、マルチモードのリアルタイムイメージング技術の開発により、各生育段階における養分の元素動態画像を取得し、収量データと合わせてコンピューターによる深層学習を行うことで、最適な施肥の数値化を図る。さらには、生育過程における1枚の可視化画像から植物内の養分、および収量予測を可能とする診断技術の開発を目指す。

  4. 放射線で光る粉を用いた植物体内元素のライブイメージング法の開発

    Grant number:20K06317  2020.4 - 2023.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    杉田 亮平

  5. イネ根圏に形成する鉄プラークがリン吸収に与える影響

    2020.4 - 2021.3

    二国間交流事業共同研究 

    杉田亮平

      More details

    Authorship:Principal investigator 

  6. ラジオアイソトープ、微少電極、蛍光プローブを相補的に用いた植物根のイオン輸送解析

    Grant number:19KK0148  2019.10 - 2024.3

    日本学術振興会  科学研究費助成事業 国際共同研究加速基金(国際共同研究強化(B))  国際共同研究加速基金(国際共同研究強化(B))

    田野井 慶太朗, 小林 奈通子, 杉田 亮平

      More details

    植物はイオン環境の急な変化に晒されると、根からのイオン吸収速度を10分以内に調節する環境応答機構をもつが、その実体は不明である。本研究では、植物が根の細胞質内イオン濃度の変化を検知しているという仮説の検証を目的として、マグネシウムイオンとナトリウムイオンの吸収速度を解析対象とし、細胞質内イオン濃度とイオン吸収速度の関係について法則性を明らかにする。そのために、①Mg2+やNa+の吸収速度が変化する条件(培地中イオン濃度と時間)において、放射性同位元素(RI)を使ったトレーサー実験で、②Mg2+やNa+の吸収速度が変化する部位を、微小イオン電極法(MIFE)で、③Mg2+やNa+の吸収速度が変化した根組織の細胞質内イオン濃度の変化を、蛍光寿命イメージング法(FLIM)で、それぞれ計測することで、根でのイオンFluxと細胞質内イオン濃度との法則性を明らかにする。
    本年度は、Mg2+についてはMIFEやFLIMでの植物の設定を想定した上で、変異体に対して28Mgを用いたトレーサー実験をおこなった。次年度MIFをタスマニア大学で実施するための準備をおこなった。
    植物の根でFLIMを実施するための環境整備をミラノ大学でおこなった。また、Mg2+を蛍光色素により検出する方法を試行したが再現性に問題があることから、Mg2+を検出する蛍光タンパク質をいくつか設定し、それらを発現させる植物の作成をスタートさせた。次年度、この植物を用いることでFLIM解析ができるものと考えている。

  7. 放射性同位元素を用いたきのこ菌糸および子実体における物質輸送の可視化技術の開発

    2018.4 - 2019.3

    公益財団法人ホクト生物科学振興財団 研究助成 

    杉田亮平

      More details

    Authorship:Principal investigator 

  8. Elucidation of substance transfer between filamentous pathogens and host plants using RI imaging

    Grant number:17K19267  2017.6 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Research (Exploratory)  Grant-in-Aid for Challenging Research (Exploratory)

    Yoshida Kentaro, TANOI Keitaro, SUGITA Ryohei

      More details

    To understand substance exchange between obligate biotroph, wheat powdery mildew Blumeria graminis f. sp tritici, and its host plant, we visualized it using RI imaging technology. We confirmed the transfer of 14C and 32P from the host plant into the wheat powdery mildew and these substances were finally accumulated in conidia of the powdery mildew. The primary leaf of the host plant was inoculated with conidia labeled with 32P, 32P was detected in other parts of the host plant, indicating that phosphate from the powdery mildew was transferred into the epidermal cells and was moved into the other tissues of the host plant with unknown mechanisms.

  9. Developing of the radioisotope-imaging system for plants

    Grant number:17K15355  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Sugita Ryohei

      More details

    In this study, we have improved a real-time radioisotope imaging system for taking fluorescence imaging. The method is to perform LED lighting for plants, pulsed fluorescence for fluorescence imaging, and radioisotope imaging during the dark period. The photosynthetic capacity of plants is reduced when pulse lighting is provided at intervals shorter than 50 msec. Therefore, we constructed a system that synchronizes a microcontroller board and a CCD camera to capture images at intervals of 50 msec. The lighting system was successfully controlled of lighting for plants and taking images in msec increments.

  10. 放射性同位元素を用いた植物体内イオンのライブイメージング技術の開発

    2017.4 - 2018.3

    一般財団法人イオン工学振興財団 研究助成 

    杉田亮平

      More details

    Authorship:Principal investigator 

  11. 農業現場における精密農業の熟成

    Grant number:15H02469  2015.4 - 2020.3

    日本学術振興会  科学研究費助成事業 基盤研究(A)  基盤研究(A)

    中西 友子, 二瓶 直登, 岩田 錬, 山岸 順子, 杉田 亮平, 廣瀬 農, 大前 芳美

      More details

    わたしたちの研究グループが開発してきた放射線RIイメージングシステム(RRIS)は、シンチレータを用いて放射線を光に変換し、その光を高感度CCDカメラで検出することで撮像可能である。撮影の視野範囲はシンチレータの大きさに依存する。これまで用いてきた板状シンチレータであるFOS(10x10㎝)を2枚並列に設置し、20x10㎝の視野範囲を撮影してきた。解析の対象としてきたイネやダイズなどは個体が小さい幼少期を用いてきたが、農業として重要となる収穫ステージは1メートル程度まで生長するため、解析を行うことができなかった。そこで視野範囲の拡大を目的とした新規シンチレータの探索を行った。様々なシンチレータの性能を評価し、最適なシンチレータの開発を行った。その結果、撮影範囲は80x60㎝に広がった。撮影には、植物から放出される自家蛍光を遮光する必要があり、自家蛍光を遮光しつつ、植物から放出される放射線が極力透過できる遮蔽材の検討を行った。その結果、2マイクロメートル厚のアルミマイラーを最適であり、自家蛍光を完全に遮光することに成功した。新しく開発した大型シンチレータを用いた撮影手法を用いて収穫時期である大型のダイズを用いて、光合成産物がどこで行われ、どこへ輸送されるのかを細かく解析した。その結果、光合成能はそれぞれの葉ごとにはあまり差がない一方で、光合成産物は古い葉は新しい葉へ積極的に輸送させること、果実は最も近い葉から供給されること等がわかってきた。今後はさらに生育ステージを細かくわけ、光合成産物の輸送を追っていくことで、効果的な施肥のタイミングを知るうえで重要となってくると考えられる。

  12. Imaging analysis of plant growth regulator using radioisotope imaging technique

    Grant number:15H04493  2015.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Tanoi Keitaro, ASAMI Tadao, NAKAMURA Hidemitsu, OKADA Kazunori, NAKANISHI Tomoko

      More details

    We applied two types of radioisotope imaging technique, live imaging technique and high resolution visualization technique to investigate auxin distribution in plant. As a result, our live imaging technique visualized 14C-auxin moving from the upper part of the plant to the root tip. The high resolution visualization technique visualized 14C-auxin at vascular bundle and vascular bundle organization. The present study showed that the radioisotope imaging techniques are able to obtain auxin distribute information.

  13. Visualization of ion movements in plants

    Grant number:15K18761  2015.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Sugita Ryohei

      More details

    To understand ion movement in plants, we have been developing an live imaging system using radioisotopes. Each ion moves via xylem and phloem in plants. To understand ion transport mechanisms in plants, it is important to study xylem and phloem flow separately. So we tried to visualize only xylem flow using heat girdling treatment. The results show that magnesium was transported mainly via the xylem, while the phloem contribution is scarce. On the other hand, phosphate transport via the phloem was slower than via the xylem.
    As a second step, we tried to visualize only xylem flow in rice in dark conditions. Magnesium and calcium transport are not influenced by light. On the other hand, phosphate transport in shoot was sensitively influenced by light. This results show that phosphate was transported via the xylem and phloem. On the other hand, the phloem contributions of magnesium, potassium, calcium, and cesium are scarce.

▼display all

 

Teaching Experience (On-campus) 1

  1. アイソトープ総合センターセミナー

    2021