受賞区分：国際学会・会議・シンポジウム等の賞  受賞国：台湾

アジア太平洋地域のバイオメカニクス分野の若手研究者賞

受賞区分：国際学会・会議・シンポジウム等の賞  受賞国：ケニア共和国

受賞区分：学会誌・学術雑誌による顕彰  受賞国：日本国

受賞区分：国際学会・会議・シンポジウム等の賞  受賞国：アイルランド

受賞区分：学会誌・学術雑誌による顕彰  受賞国：日本国

受賞区分：国内学会・会議・シンポジウム等の賞 

受賞区分：国内学会・会議・シンポジウム等の賞  受賞国：日本国

受賞区分：国内学会・会議・シンポジウム等の賞  受賞国：日本国

受賞区分：国内学会・会議・シンポジウム等の賞  受賞国：日本国

受賞区分：国内学会・会議・シンポジウム等の賞  受賞国：日本国

受賞区分：国内学会・会議・シンポジウム等の賞  受賞国：日本国

受賞区分：国内学会・会議・シンポジウム等の賞 

記述言語：英語   出版者・発行元：Biochemical and Biophysical Research Communications  

Understanding in multicellular behaviors in three-dimensional (3D) culture models such as organoids is important to help us better comprehend the mechanisms of the morphogenesis and functions of diverse organs in vivo cellular environment. In this study, we elucidated the multicellular behaviors of the osteocytic spheroids in response to the chemically induced osteogenesis supplements (OS). Particularly, we conducted 1) size change measurement, 2) fusion experiment, and 3) collagen embedding experiment of spheroids, in response to the OS. We found out that the OS alters the multicellular behaviors of the spheroid by greater reduction in the size change measurement and slowing down the speed of fusion experiment and collagen embedding experiment of the spheroids. We also highlighted that the driving force of these changes was the tight actin filaments generated on the surface of the spheroids. Hence, the results altogether indicate that the spheroid model exerted the different multicellular behaviors against the differentiation capability. This study will contribute to understanding the multicellular behaviors of the 3D culture model reconstructed by the cells with greater cell-cell interaction force.

DOI： 10.1016/j.bbrc.2022.07.026

Web of Science

Scopus

PubMed

記述言語：英語   出版者・発行元：Journal of the Mechanical Behavior of Biomedical Materials  

The deformation of the cell nucleus may cause dispersion of chromatin and eventually enhance transcription, translation, and protein expression. If this happens in the hypertensive artery, an excessive stretch of smooth muscle cell (SMC) nuclei caused by hypertension may provoke wall thickening. Here, we measured deformation of SMC nuclei in rabbit thoracic aortas stretched in different directions. Thin 0.2-mm-thick specimens were sliced in the direction perpendicular to their axial and circumferential directions, and stretched in the circumferential and axial directions, respectively. The deformation of the actin filament (AF) network was similar to that of the whole tissue, whereas the deformation of the nucleus was significantly smaller than the others. Notably, the nucleus seldom deformed when the tissue was stretched in the axial direction. A novel cell model in which the nucleus is connected to the extracellular matrix via the AF network successfully explained the relative unresponsiveness of the nucleus to the axial stretch. It has been pointed out that stress is maintained constant in the circumferential direction but not in the axial direction in the artery wall during hypertension. The relative unresponsiveness of the nucleus to the axial stretch represented in this study explains this phenomenon.

DOI： 10.1016/j.jmbbm.2022.105326

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PubMed

記述言語：英語  

DOI： 10.1016/j.jbiosc.2021.09.007

PubMed

記述言語：英語   出版者・発行元：Japanese Society for Medical and Biological Engineering  

<p>Observing cell nuclei in the 3D culture model was still challenging due to the limited penetration depth of lasers from the microscopes. Observation of deformation of cell nuclei is important because it is thought to modulate cellular functions. In this study, we have achieved optical clearing techniques for osteocytic spheroids using OptiPrep. Furthermore, we developed a novel 3D image analysis software to quantify the shape and position of the nuclei in the spheroids. As a result, we found out that the shape of cell nuclei was more sphere-shaped inside the spheroid. The combination of optical clearing and 3D image analysis techniques is expected to become powerful tools in the field of 3D culture models.</p>

DOI： 10.11239/jsmbe.annual60.287

CiNii Research

記述言語：英語   出版者・発行元：Japanese Society for Medical and Biological Engineering  

<p>Observing cell nuclei in the 3D culture model was still challenging due to the limited penetration depth of lasers from the microscopes. Observation of deformation of cell nuclei is important because it is thought to modulate cellular functions. In this study, we have achieved optical clearing techniques for osteocytic spheroids using OptiPrep. Furthermore, we developed a novel 3D image analysis software to quantify the shape and position of the nuclei in the spheroids. As a result, we found out that the shape of cell nuclei was more sphere-shaped inside the spheroid. The combination of optical clearing and 3D image analysis techniques is expected to become powerful tools in the field of 3D culture models.</p>

DOI： 10.11239/jsmbe.annual60.161_2

CiNii Research

記述言語：英語   出版者・発行元：Japanese Society for Medical and Biological Engineering  

<p>While the osteocyte plays an important role as control tower to modulate the bone remodeling, our group has developed a novel 3D osteocytic spheroid model reconstructed by human mesenchymal stem cells. To characterize the mechanical behaviors of the osteocytic spheroids, we carried out time-dependent 1) size measurement, 2) fusion experiment, and 3) embedding experiment of spheroids in collagen matrix in response to the chemical osteogenesis induction. From these experiments, we found out that the spheroids exhibited distinct mechanical behaviors in the presence of chemical osteogenesis supplements. Most notably, we suggested that those change was provoked by actin filaments generated on the surface of the spheroids. Our present findings will contribute to understanding in mechanical behaviors of 3D culture model with greater cell-cell interaction force in vitro or in vivo model after transplantation.</p>

DOI： 10.11239/jsmbe.annual60.155_1

CiNii Research

記述言語：日本語   出版者・発行元：一般社団法人 日本機械学会  

DOI： 10.1299/jsmemecj.2022.s021-08

CiNii Research

記述言語：日本語   出版者・発行元：一般社団法人 日本機械学会  

DOI： 10.1299/jsmebiofro.2022.33.2d03

CiNii Research

記述言語：日本語   出版者・発行元：一般社団法人 日本機械学会  

DOI： 10.1299/jsmebiofro.2022.33.1a02

CiNii Research

記述言語：日本語   出版者・発行元：一般社団法人 日本機械学会  

DOI： 10.1299/jsmebiofro.2022.33.2c06

CiNii Research

記述言語：日本語   出版者・発行元：一般社団法人 日本機械学会  

DOI： 10.1299/jsmebiofro.2022.33.1e10

CiNii Research

記述言語：日本語   出版者・発行元：一般社団法人 日本機械学会  

DOI： 10.1299/jsmebiofro.2022.33.2d02

CiNii Research

記述言語：日本語   出版者・発行元：一般社団法人 日本機械学会  

DOI： 10.1299/jsmebiofro.2022.33.1e23

CiNii Research

記述言語：日本語   出版者・発行元：一般社団法人 日本機械学会  

DOI： 10.1299/jsmebiofro.2022.33.1e16

CiNii Research

記述言語：英語   出版者・発行元：Journal of Bioscience and Bioengineering  

Osteocytes play an important role to modulate the bone remodeling and are also known as terminally differentiated cells originated from the osteoblast precursor cells, but its differentiation mechanism remains unclear. Since an efficient in vitro method to evoke the osteocyte differentiation from the osteoblast precursor cells has not been established, we conducted the comparative gene expression analysis for mouse pre-osteoblast MC3T3-E1 cells in order to elucidate the key factors to induce the osteocyte differentiation from the pre-osteoblast cells. In this study, we prepared four different culture environments by modulating their cell-substrate interaction and cell–cell interaction; (i) low and (ii) high cell density on the adhesive culture models, and (iii) low and (iv) high cell density on the non-adhesive floating culture models. By comparing these conditions in terms of cell-substrate and cell–cell interaction, we showed that the elimination of cell-substrate interaction under non-adhesive floating culture greatly up-regulated the gene expression of osteocyte markers in the pre-osteoblast cells. Moreover, the presence of moderate cell–cell interaction in the non-adhesive spheroid culture further enhanced the up-regulation of osteocyte markers for the pre-osteoblast cells. The results altogether suggest the most appropriate culture environment to induce the in vitro osteocyte differentiation of pre-osteoblast cells.

DOI： 10.1016/j.jbiosc.2021.09.004

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PubMed

記述言語：英語  

DOI： 10.1089/ten.TEA.2020.0228

PubMed

記述言語：英語   出版者・発行元：Scientific Reports  

Osteocytes are mechanosensory commander cells to regulate bone remodeling throughout the lifespan. While the osteocytes are known as terminally differentiated cells derived from mesenchymal stem cells, the detailed mechanisms of osteocyte differentiation remain unclear. In this study, we fabricated 3D self-organized spheroids using human mesenchymal stem cells (MSCs). Under the osteogenesis induction medium, the spheroid culture model exerted the osteocyte-likeness within 2 days compared to a conventional 2D monolayer model. Moreover, we showed that an inhibition of actin polymerization in the spheroid further up-regulated the osteocyte gene expressions. Notably, we represented that the cell condensed condition acquired in the 3D spheroid culture model determined a differentiation fate of MSCs to osteocytes. Taken together, we suggest that our self-organized spheroid model can be utilized as a new in vitro model to represent the osteocyte and to recapitulate an in vitro ossification process.

DOI： 10.1038/s41598-021-92607-z

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PubMed

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1038/s41598-021-88505-z

Web of Science

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1299/jbse.20-00227

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1038/s41598-020-65884-3

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.3389/fbioe.2019.00288

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（国際会議プロシーディングス）  

DOI： https://doi.org/10.1109/LifeTech52111.2021.9391919

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1299/jbse.19-00097

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1371/journal.pone.0185394. eCollection 2017.

記述言語：英語   掲載種別：研究論文（学術雑誌）  

担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1080/09168451.2014.975184.

開催年月日： 2022年11月

記述言語：英語  

開催地：Sydney   国名：オーストラリア連邦  

開催年月日： 2022年10月

記述言語：英語   会議種別：口頭発表（一般）  

開催地：Jeju   国名：大韓民国  

開催年月日： 2021年12月

記述言語：英語   会議種別：ポスター発表  

開催地：Kyoto, Japan  

開催年月日： 2021年7月

記述言語：英語   会議種別：口頭発表（一般）  

国名：イタリア共和国  

開催年月日： 2021年7月

記述言語：英語   会議種別：口頭発表（招待・特別）  

国名：日本国  

開催年月日： 2023年11月

記述言語：英語   会議種別：口頭発表（一般）  

開催年月日： 2023年10月

開催年月日： 2023年9月

記述言語：日本語  

開催年月日： 2023年3月

記述言語：日本語   会議種別：口頭発表（一般）  

国名：日本国  

開催年月日： 2022年9月

記述言語：日本語   会議種別：口頭発表（一般）  

開催年月日： 2022年7月

記述言語：英語   会議種別：口頭発表（一般）  

開催地：Taiwan   国名：台湾  

開催年月日： 2022年6月

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：新潟   国名：日本国  

開催年月日： 2022年6月

記述言語：日本語   会議種別：口頭発表（一般）  

開催地：神奈川   国名：日本国  

記述言語：日本語   会議種別：口頭発表（一般）  

国名：日本国  

記述言語：日本語   会議種別：口頭発表（一般）  

国名：日本国  

記述言語：日本語   会議種別：ポスター発表  

国名：日本国