Faculty Profiles - KIM Jeonghyun

写真a

KIM Jeonghyun

Organization

Graduate School of Engineering Mechanical Systems Engineering 1 Assistant Professor

Graduate School

Graduate School of Engineering

Undergraduate School

School of Engineering Mechanical and Aerospace Engineering

Contact information

Homepage

https://biomech-lab.com/

Other name(s)

KIM Jeonghyun

External link

Degree 3

Doctor of Philosophy （ 2017.9 The University of Tokyo ）
Master of Engineering （ 2014.9 The University of Tokyo ）
Bachelor of Engineering （ 2010.5 The University of Sydney ）

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Research Interests 5

regenerative medicine
tissue engineering
biomedical engineering
biomechanics
mechanobiology

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Research Areas 3

Life Science / Biomedical engineering
Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering
Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Mechanics of materials and materials

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Research History 3

Nagoya University Assistant Professor

2021.4
Kyoto University

2018.11 - 2021.3

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Country：Japan
The University of Tokyo

2017.4 - 2018.10

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Country：Japan

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Education 3

The University of Tokyo Graduate School of Engineering Mechanical Engineering

2014.10 - 2017.9

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Country： Japan
The University of Tokyo Graduate School of Engineering Mechanical Engineering

2012.10 - 2014.9

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Country： Japan
The University of Sydney Faculty of Engineering Mechanical Engineering (Biomedical)

2006.3 - 2010.5

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Country： Australia

Notes： Honours degreee

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Professional Memberships 3

日本生体医工学会
日本再生医療学会
日本機械学会

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Committee Memberships 9

日本機械学会第36回バイオエンジニアリング講演会実行委員会

2023.12 - 2024.5

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Committee type：Academic society
日本機械学会バイオエンジニアリング講演会『静水圧メカノバイオロジー』セッションオーガナイザー

2023.11

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Committee type：Academic society
日本機械学会年次大会『細胞メカニクス』セッションオーガナイザー

2022.10

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Committee type：Academic society
日本機械学会バイオエンジニアリング部門広報委員

2022.4 - 2024.3

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Committee type：Academic society
日本機械学会バイオエンジニアリング部門若手による次世代戦略委員会

2022.4 - 2024.3

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Committee type：Academic society
The 32nd Biofrontier conference Steering committee member

2021.9 - 2022.1

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Committee type：Academic society
The 11th Asian-Pacific Conference on Biomechanics Steering committee member

2021.6 - 2021.12

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Committee type：Academic society
The Korean Institute of Chemical Engineering (Japan region) Director of Business Affairs

2021.1 - 2021.12

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Committee type：Academic society
The Korean Scientists and Engineers Associate in Japan Bioengineering subcommittee chair

2018.7 - 2021.3

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Committee type：Academic society

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Awards 20

2023年度日本生体医工学会東海支部研究奨励賞

2023.10 日本生体医工学会静水圧に応答する3次元培養骨細胞モデル

キム・ジョンヒョン，新岡琴音，前田英次郎，松本健郎

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Award type：Award from Japanese society, conference, symposium, etc.
日本機械学会東海支部奨励賞

2022.3 日本機械学会東海支部

Jeonghyun Kim

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Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
The Outstanding Abstract Award Runner-up

2021.12 The 11th Asian-Pacific Conference on Biomechanics

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Award type：Award from international society, conference, symposium, etc. Country：Japan
2020 JBSE Graphics of the Year Award

2021.6 Journal of Biomechanical Science and Engineering

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Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
2020 JBSE Papers of the Year Award

2021.6 Journal of Biomechanical Science and Engineering

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Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
The Yamaguchi Medal

2019.11 Asian-Pacific Conference on Biomechanics

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Award type：Award from international society, conference, symposium, etc. Country：Taiwan, Province of China

Awarded for outstanding young investigator in soft tissue mechanics
The Best Oral Presentation Award

2019.8 The 4th Africa International Biotechnology and Biomedical Conference

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Award type：Award from international society, conference, symposium, etc. Country：Kenya
The Academic Excellence Award

2019.2 Korean Scientists and Engineers Association in Japan

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Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
The Outstanding Young Researcher Presentation Award

2018.7 The 8th World Congress of Biomechanics

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Award type：Award from international society, conference, symposium, etc. Country：Ireland
The Best Paper Award

2016.10 Japanese Journal of Clinical Biomechanics

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Award type：Honored in official journal of a scientific society, scientific journal Country：Japan
若手優秀講演賞

2023.12 日本機械学会第34回バイオフロンティア講演会摺鉢面上のMC3T3-E1細胞挙動に及ぼす細胞内張力の影響

石黒　恵奨，王　軍鋒，キム　ジョンヒョン，前田　英次郎，松本　健郎

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Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
The Best Presentation Award

2023.3 日本機械学会東海支部第54回学生員卒業研究発表講演会繰返引張下の重層化したMC3T3-E1細胞の上下層で異なる配向の原因解明に関する研究

鈴木秀一郎，王軍鋒，キム・ジョンヒョン，前田英次郎，松本健郎
The Best Presentation Award

2023.3 日本機械学会東海支部第72期総会・講演会 TOKAI ENGINEERING COMPLEX 2023 (TEC23) スフェロイド培養法によるマウス軟骨前駆細胞の肥大化軟骨細胞分化誘導

富田航世，キム・ジョンヒョン，前田英次郎，松本健郎
若手優秀講演賞

2022.12 日本機械学会第33回バイオフロンティア講演会電界紡糸法により成膜したエラスチン組織の力学挙動解析

篠川晃佑，キム・ジョンヒョン，鳴瀧彩絵，松本健郎，前田英次郎

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Award type：Award from Japanese society, conference, symposium, etc.
The Outstanding Student Poster Award

2022.6 日本機械学会第34回バイオエンジニアリング講演会マウス軟骨前駆細胞の３次元スフェロイド培養による肥大軟骨分化およびアポトーシス誘導

富田航世,キム・ジョンヒョン,前田英次郎,松本健郎

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Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
The Outstanding Student Poster Award

2022.6 日本機械学会第34回バイオエンジニアリング講演会腱 Elastic recoil 挙動におけるコラーゲンとエラスチンの役割解明を目的としたクリンプ構造の評価と動的力学特性の解析

石崎裕聖,キム・ジョンヒョン,松本健郎,前田英次郎

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Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
優秀講演賞

2022.3 日本機械学会東海支部第53回学生員卒業研究発表講演会イベリアトゲイモリ大腿骨損傷部の再生現象解明に関する研究

伊藤優作, キムジョンヒョン, 松本健郎, 前田英次郎

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Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
優秀講演賞

2022.3 日本機械学会東海支部第53回学生員卒業研究発表講演会静水圧刺激がマウス骨芽前駆細胞の細胞形状・増殖に与える影響

水島慎吾, キムジョンヒョン, 前田英次郎, 松本健郎

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Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
優秀講演賞

2022.3 日本機械学会東海支部第53回学生員卒業研究発表講演会組織透明化手法による３次元骨細胞スフェロイド内部の細胞核形状評価

稲垣貴士, キムジョンヒョン, 前田英次郎, 松本健郎

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Award type：Award from Japanese society, conference, symposium, etc. Country：Japan
The Outstanding Student Presentation Award

2022.1 The 32nd Biofrontier conference Elucidation of mechanical characteristics of Xenopus laevis embryos by seamless uniaxial tension-compression test

Ryosuke Saito, Hiromasa Ninomiya, Jeonghyun Kim, Eijiro Maeda, Atsuaka Tamura, Takeo Matsumoto

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Award type：Award from Japanese society, conference, symposium, etc.

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Papers 24

Novel air-liquid interface culture model to investigate stiffness-dependent behaviors of alveolar epithelial cells.

Takahashi Y, Ito S, Wang J, Kim J, Matsumoto T, Maeda E

Biochemical and biophysical research communications Vol. 708 page： 149791 2024.3

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Language：English

DOI： 10.1016/j.bbrc.2024.149791

PubMed
Contributions of collagen and elastin to elastic behaviours of tendon fascicle

Ishizaki Y., Wang J., Kim J., Matsumoto T., Maeda E.

Acta Biomaterialia Vol. 176 page： 334 - 343 2024.3

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Language：English Publisher：Acta Biomaterialia

Tendon exhibits the capacity to be stretched and to return to its original length without suffering structural damage in vivo, a capacity known as elastic recoil. Collagen fibres are aligned longitudinally and elastin fibres mostly run parallel to collagen fibres in tendon. However, their interactions and contributions to tendon elastic behaviours are not well understood. The present study examined functional roles of collagen and elastin in tendon elastic behaviours using a variety of mechanical tests. We prepared three types of fascicle specimens from mouse tail tendon: fascicles freshly isolated, those digested with elastase in PBS to selectively remove elastin, and those incubated in PBS without elastase. A quasi-static tensile test demonstrated that elastase-treated fascicles had higher tangent moduli and strength compared to fresh and PBS fascicles. Cyclic stretching tests showed that fresh and PBS fascicles could withstand cyclic strain at both small and large amplitudes, but elastase-treated fascicles could only behave elastically to a limited degree. Fibre-sliding analysis revealed that fresh fascicles could be elongated both through stretching of collagen fibers and through movement of the fibres. However, elastase-treated fascicles could be stretched only via fibre stretching. This evidence suggests that normal tendons can be extended through both fibre stretching and fibre sliding, whereas tendons without elastin can only extend as much as collagen fibers can withstand. Accordingly, collagen fibres mainly contribute to tendon elastic behaviours by furnishing rigidity and elasticity, whereas elastin provides tendon viscoelasticity and also enables sliding of collagen fibres during elastic behaviours. Statement of significance: The present study revealed distinct mechanical functions of collagen and elastin fibres in elastic behaviours of mouse tail tendon fascicle using a variety of mechanical tests at both microscopic and macroscopic levels. It was demonstrated that collagen mainly governs tendon fascicle rigidity and elasticity, but only possesses limited extensibility, whereas elastin contributes to viscoelasticity and collagen fibre sliding, enabling elastic recoil behaviour against relatively large deformation. By their interactions, tendon can be elongated without suffering major structural damage and withstand a large magnitude of tensile force in response to mechanical loading. Such information should be particularly useful in designing collagen-based biomaterials such as artificial tendons, in that previous studies have merely considered collagen without incorporation of elastin.

DOI： 10.1016/j.actbio.2024.01.014

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PubMed
Biomechanical analysis of tendon regeneration capacity of Iberian ribbed newts following transection injury: Comparison to a mouse model

Sato, F; Masuda, Y; Suzuki, D; Hayashi, T; Iwasaki, T; Kim, J; Matsumoto, T; Maeda, E

JOURNAL OF ORTHOPAEDIC RESEARCH Vol. 42 ( 3 ) page： 607 - 617 2023.11

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Language：English Publisher：Journal of Orthopaedic Research

Adult mammals are known for their poor ability to regenerate tissues, including tendons. On the other hand, urodeles have become an important model in regenerative studies for their remarkable ability to regenerate various body parts and organs throughout life, such as limbs, retinas, or even the brain. However, little is known about their capacity to regenerate injured tendons. If newts can also repair tendons without scar formation, they may be a suitable animal model for tendon regeneration studies in other adult vertebrates. Therefore, the present study used Iberian ribbed newts to characterize mechanical and structural regeneration of tendons following transection, using tensile tests and multiphoton microscopy. A digital flexor tendon in a hindlimb was transected either partially or completely, and regenerated tendon was examined 6 and 12 weeks after the operation. Tensile strength of regenerated tendons was significantly less than normal at 6 weeks, but was remarkably recovered at 12 weeks, reaching levels comparable to those of uninjured tendons. On the other hand, mouse tendons demonstrated poor recovery of strength even after 12 weeks. Multiphoton microscopy revealed that tendon-like collagenous tissue bridges residual tendon stubs in newts, but disorganized scar-like tissue filled the injured location in mice. These findings highlight the remarkable capacity of newts to recover from tendon injury and confirm the utility of newts as a model to study tendon regeneration.

DOI： 10.1002/jor.25705

Web of Science

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PubMed
3D quantitative assessment for nuclear morphology in osteocytic spheroid with optical clearing technique

Inagaki, T; Kim, J; Tomida, K; Maeda, E; Matsumoto, T

INTEGRATIVE BIOLOGY Vol. 15 2023.4

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Language：English Publisher：Integrative biology : quantitative biosciences from nano to macro

In recent years, three-dimensional (3D) cell culture has been attracting attention as a cell culture model that mimics an environment closer to that of a living organism. It is known that there is a close relationship between cell nuclear shape and cellular function, which highlights the importance of cell nucleus shape analysis in the 3D culture. On the other hand, it is difficult to observe the cell nuclei inside the 3D culture models because the penetration depth of the laser light under a microscope is limited. In this study, we adopted an aqueous iodixanol solution to the 3D osteocytic spheroids derived from mouse osteoblast precursor cells to make the spheroids transparent for 3D quantitative analysis. With a custom-made image analysis pipeline in Python, we found that the aspect ratio of the cell nuclei near the surface of the spheroid was significantly greater than that at the center, suggesting that the nuclei on the surface were deformed more than those at the center. The results also quantitatively showed that the orientation of nuclei in the center of the spheroid was randomly distributed, whereas those on the surface of the spheroid were oriented parallel to the surface of the spheroid. Our 3D quantitative method with an optical clearing technique will contribute to the 3D culture models including various organoid models to elucidate the nuclear deformation during the development of the organs. Insight box Although 3D cell culture has been a powerful tool in the fields of fundamental biology and tissue engineering, it raises the demand for quantification techniques for cell nuclear morphology in the 3D culture model. In this study, we attempted to optically clear a 3D osteocytic spheroid model using iodixanol solution for the nuclear observation inside the spheroid. Moreover, using a custom-made image analysis pipeline in Python, we successfully quantified the nuclear morphology regarding aspect ratio and orientation. Our quantitative method with the optical clearing technique will contribute to the 3D culture models such as various organoid models to elucidate the nuclear deformation during the development of the organs.

DOI： 10.1093/intbio/zyad007

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PubMed
Spheroid culture for chondrocytes triggers the initial stage of endochondral ossification

Kim, J; Tomida, K; Matsumoto, T; Adachi, T

BIOTECHNOLOGY AND BIOENGINEERING Vol. 119 ( 11 ) page： 3311 - 3318 2022.11

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Language：English Publisher：Biotechnology and Bioengineering

Endochondral ossification is the process of bone formation derived from growing cartilage duringskeletal development. In previous studies, we provoked the osteocyte differentiation of osteoblast precursor cells under a three-dimensional (3D) culture model. To recapitulate the endochondral ossification, the present study utilized the self-organized scaffold-free spheroid model reconstructed by pre-chondrocyte cells. Within 2-day cultivation in the absence of the chemically induced chondrogenesis supplements, the chondrocyte marker was greatly expressed in the inner region of the spheroid, whereas the hypertrophic chondrocyte marker was strongly detected in the surface region of the spheroid. Notably, we found out that the gene expression levels of osteocyte markers were also greatly upregulated compared to the conventional 2D monolayer. Moreover, after long-term cultivation for 28 days, it induced morphological changes in the spheroid, such as cellular hypertrophy and death. In this study, in order to recapitulate the initial stage of the endochondral ossification, we highlighted the potentials of the 3D culture method to drive the hypertrophic chondrocyte differentiation of the pre-chondrocyte cells.

DOI： 10.1002/bit.28203

Web of Science

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PubMed
Induction of osteocyte differentiation for mesenchymal stem cells in 3D scaffold-free spheroid culture

Kim, J; Adachi, T; Matsumoto, T

TISSUE ENGINEERING PART A Vol. 28 page： 325 - 325 2022.10

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Web of Science
Effect of chemically induced osteogenesis supplements on multicellular behavior of osteocytic spheroids

Kim, J; Inagaki, T; Sunaga, J; Adachi, T; Matsumoto, T

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Vol. 622 page： 79 - 85 2022.9

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Language：English Publisher：Biochemical and Biophysical Research Communications

Understanding in multicellular behaviors in three-dimensional (3D) culture models such as organoids is important to help us better comprehend the mechanisms of the morphogenesis and functions of diverse organs in vivo cellular environment. In this study, we elucidated the multicellular behaviors of the osteocytic spheroids in response to the chemically induced osteogenesis supplements (OS). Particularly, we conducted 1) size change measurement, 2) fusion experiment, and 3) collagen embedding experiment of spheroids, in response to the OS. We found out that the OS alters the multicellular behaviors of the spheroid by greater reduction in the size change measurement and slowing down the speed of fusion experiment and collagen embedding experiment of the spheroids. We also highlighted that the driving force of these changes was the tight actin filaments generated on the surface of the spheroids. Hence, the results altogether indicate that the spheroid model exerted the different multicellular behaviors against the differentiation capability. This study will contribute to understanding the multicellular behaviors of the 3D culture model reconstructed by the cells with greater cell-cell interaction force.

DOI： 10.1016/j.bbrc.2022.07.026

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PubMed
Dependency of deformation of cell nucleus on stretch direction of tissue: Relation to anisotropic response of aortic media to hypertension

Fan, Y; Wang, JF; Kim, J; Maeda, E; Matsumoto, T

JOURNAL OF THE MECHANICAL BEHAVIOR OF BIOMEDICAL MATERIALS Vol. 133 page： 105326 2022.9

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Language：English Publisher：Journal of the Mechanical Behavior of Biomedical Materials

The deformation of the cell nucleus may cause dispersion of chromatin and eventually enhance transcription, translation, and protein expression. If this happens in the hypertensive artery, an excessive stretch of smooth muscle cell (SMC) nuclei caused by hypertension may provoke wall thickening. Here, we measured deformation of SMC nuclei in rabbit thoracic aortas stretched in different directions. Thin 0.2-mm-thick specimens were sliced in the direction perpendicular to their axial and circumferential directions, and stretched in the circumferential and axial directions, respectively. The deformation of the actin filament (AF) network was similar to that of the whole tissue, whereas the deformation of the nucleus was significantly smaller than the others. Notably, the nucleus seldom deformed when the tissue was stretched in the axial direction. A novel cell model in which the nucleus is connected to the extracellular matrix via the AF network successfully explained the relative unresponsiveness of the nucleus to the axial stretch. It has been pointed out that stress is maintained constant in the circumferential direction but not in the axial direction in the artery wall during hypertension. The relative unresponsiveness of the nucleus to the axial stretch represented in this study explains this phenomenon.

DOI： 10.1016/j.jmbbm.2022.105326

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PubMed
Internal radial perfusion bioreactor promotes decellularized and recellularization of rat uterine tissue. Reviewed

Narintadeach Charoensombut , Kinyoshi Kawabata, Jeonghyun Kim, Minki Chang, Tsuyoshi Kimura, Akio Kishida, Takashi Ushida, Katsuko Furukawa

Journal of Bioscience and Bioengineering Vol. 133 ( 1 ) page： 83 - 88 2022.1

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Language：English

DOI： 10.1016/j.jbiosc.2021.09.007

PubMed
Comparative gene expression analysis for pre-osteoblast MC3T3-E1 cells under non-adhesive culture toward osteocyte differentiation Reviewed

Kim, J; Kigami, H; Adachi, T

JOURNAL OF BIOSCIENCE AND BIOENGINEERING Vol. 132 ( 6 ) page： 651 - 656 2021.12

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Language：English Publisher：Journal of Bioscience and Bioengineering

Osteocytes play an important role to modulate the bone remodeling and are also known as terminally differentiated cells originated from the osteoblast precursor cells, but its differentiation mechanism remains unclear. Since an efficient in vitro method to evoke the osteocyte differentiation from the osteoblast precursor cells has not been established, we conducted the comparative gene expression analysis for mouse pre-osteoblast MC3T3-E1 cells in order to elucidate the key factors to induce the osteocyte differentiation from the pre-osteoblast cells. In this study, we prepared four different culture environments by modulating their cell-substrate interaction and cell–cell interaction; (i) low and (ii) high cell density on the adhesive culture models, and (iii) low and (iv) high cell density on the non-adhesive floating culture models. By comparing these conditions in terms of cell-substrate and cell–cell interaction, we showed that the elimination of cell-substrate interaction under non-adhesive floating culture greatly up-regulated the gene expression of osteocyte markers in the pre-osteoblast cells. Moreover, the presence of moderate cell–cell interaction in the non-adhesive spheroid culture further enhanced the up-regulation of osteocyte markers for the pre-osteoblast cells. The results altogether suggest the most appropriate culture environment to induce the in vitro osteocyte differentiation of pre-osteoblast cells.

DOI： 10.1016/j.jbiosc.2021.09.004

Web of Science

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PubMed
Modulation of <i>Sost</i> Gene Expression Under Hypoxia in Three-Dimensional Scaffold-Free Osteocytic Tissue. Reviewed

Kim J, Adachi T

Tissue engineering. Part A Vol. 27 ( 15-16 ) page： 1037 - 1043 2021.8

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Language：English

DOI： 10.1089/ten.TEA.2020.0228

PubMed
Cell-fate decision of mesenchymal stem cells toward osteocyte differentiation is committed by spheroid culture Reviewed

Jeonghyun Kim, Taiji Adachi

Scientific Reports Vol. 11 ( 1 ) page： 13204 2021.6

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Language：English

DOI： 10.1038/s41598-021-92607-z

Web of Science

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PubMed
Uniaxially-fixed mechanical boundary condition elicits cellular alignment in collagen matrix with induction of osteogenesis Reviewed

Jeonghyun Kim, Keiichi Ishikawa, Junko Sunaga, Taiji Adachi

Scientific Reports Vol. 11 ( 9009 ) 2021.4

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： https://doi.org/10.1038/s41598-021-88505-z
Intracellular tension of osteoblast in collagen gel elicits osteocyte alignment under uniaxially-fixed boundary condition

Kim, J; Ishikawa, K; Sunaga, J; Adachi, T

BIORHEOLOGY Vol. 58 ( 3-4 ) page： 161 - 161 2021

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Web of Science
Characterization of self-organized osteocytic spheroids using mouse osteoblast-like cells Invited Reviewed

Jeonghyun Kim, Hiroyuki Kigami, Taiji Adachi

Journal of Biomechanical Science and Engineering 2020.6

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： https://doi.org/10.1299/jbse.20-00227
Acquired contractility ability in human endometrial stromal cells by passive loading of cyclic tensile stretch Reviewed

Jeonghyun Kim, Takashi Ushida, Kevin Montagne, Yasushi Hirota, Osamu Yoshino, Takehiro Hiraoka, Yutaka Osuga, Katsuko Furukawa

2020.6

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： https://doi.org/10.1038/s41598-020-65884-3
Cell condensation triggers the differentiation of osteoblast precursor cells to osteocyte-like cells Reviewed

Jeonghyun Kim, Taiji Adachi

Vol. 7 ( 288 ) 2019.10

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： https://doi.org/10.3389/fbioe.2019.00288
Dependency of deformation of cell nucleus on stretch direction of tissue: Relation to anisotropic response of aortic media to hypertension Reviewed

Yong Fan, Junfeng Wang, Jeonghyun Kim, Eijiro Maeda, Takeo Matsumoto

Journal of the Mechanical Behavior of Biomedical Materials Vol. 133 ( 105326 ) 2022.9
Three-dimensional culture technology: Self-organized spheroid culture drives osteocytogenesis Reviewed

Jeonghyun Kim, Taiji Adachi

2021.4

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Authorship：Lead author Language：English Publishing type：Research paper (international conference proceedings)

DOI： https://doi.org/10.1109/LifeTech52111.2021.9391919
Fabrication of extracellular matrix hydrogel derived from uterine tissues Reviewed

Kinyoshi Kawabata, Narintadeach Charoensombut, Jeonghyun Kim, Tsuyoshi Kimura, Akio Kishida, Takashi Ushida, Katsuko Furukawa

Vol. 41 page： 287 - 292 2020

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Language：Japanese Publishing type：Research paper (scientific journal)
Fabrication of uterine decellularized matrix using high hydrostatic pressure through depolymerization of actin filaments Invited Reviewed

Jeonghyun Kim, Shu Takeda, Narintadeach Charoensombut, Kinyoshi Kawabata, Yugo Kishimoto, Tsuyoshi Kimura, Akio Kishida, Takashi Ushida, Katsuko Furukawa

Vol. 14 ( 3 ) page： 19-00097 2019.9

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： https://doi.org/10.1299/jbse.19-00097
Hypergravity down-regulates c-fos gene expression via ROCK/Rho-GTP and the PI3K signaling pathway in murine ATDC5 chondroprogenitor cells Reviewed

Jeonghyun Kim, Kevin Montagne, Hidetoshi Nemoto, Takashi Ushida, Katsuko Furukawa

Vol. 12 ( 9 ) page： e0185394 2017.9

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： https://doi.org/10.1371/journal.pone.0185394. eCollection 2017.
Involvement of the mTOR pathway in redifferentiation of bovine chondrocytes under dynamic hydrostatic loading Reviewed

Kevin Montagne, Dongig Oh, Jeonghyun Kim, Takashi Ushida, Katsuko S Furukawa

Vol. 37 page： 1 - 8 2016.9

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Language：English Publishing type：Research paper (scientific journal)
Enhanced chondrogenesis with upregulation of PKR using a novel hydrostatic pressure bioreactor Reviewed

Jeonghyun Kim, Kevin Montagne, Takashi Ushida, Katsuko S Furukawa

Vol. 79 ( 2 ) page： 239 - 241 2014.10

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Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

DOI： https://doi.org/10.1080/09168451.2014.975184.

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Books 1

最先端ナノライフシステム研究

キムジョンヒョン，前田英次郎，松本健郎（ Role： Contributor , 力で組織・細胞を制御する）

丸善プラネット 2022

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Book type：Textbook, survey, introduction

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Presentations 21

Biomechanical analysis in spheroid culture: Induction of hypertrophic chondrocyte differentiation International conference

Jeonghyun Kim, Kosei Tomida, Eijiro Maeda, Taiji Adachi, Takeo Matsumoto

The 12th Asian-Pacific Conference on Biomechanics 2023.11.16

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Event date： 2023.11

Language：English Presentation type：Oral presentation (general)
Application of hydrostatic pressure to facilitate the osteocyte differentiation in spheroid culture Invited

Jeonghyun Kim, Kotone Niioka, Eijro Maeda, Taiji Adachi, Takeo Matsumoto

The Korean Society of Mechanical Engineers Annual Meeting 2023.11.3

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Event date： 2023.11

Language：English Presentation type：Oral presentation (invited, special)
Cellular biomechanics using 3D spheroid culture for bone study Invited

Jeonghyun Kim

日本機械学会 2023年度年次大会 2023.9.3

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Event date： 2023.9

Language：English Presentation type：Oral presentation (invited, special)
Biomechanical analysis of osteocytic spheroids for bone tissue engineering applications Invited International conference

Jeonghyun Kim , Takashi Inagaki , Eijiro Maeda , Taiji Adachi , Takeo Matsumoto

The International Conference on Precision Engineering and Sustainable Manufacturing 2023 (PRESM2023) 2023.7.19

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Event date： 2023.7

Language：English Presentation type：Oral presentation (invited, special)

Venue：Okinawa Country：Japan
Actin filaments in response to chemical osteogenesis supplements alter the multicellular behavior of osteocytic spheroids International conference

Jeonghyun Kim, Taiji Adachi, Takeo Matsumoto

The 28th Congress of the European Society of Biomechanics 2023.7.11

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Event date： 2023.7

Language：English Presentation type：Poster presentation

Venue：Maastricht Country：Netherlands
Measurement of multicellular behavior and mechanical properties in 3D osteocytic spheroids Invited

キムジョンヒョン, 稲垣貴士, 前田英次郎, 安達泰治, 松本健郎

第62回日本生体医工学会大会 2023.5.20 日本生体医工学会

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Event date： 2023.5

Language：Japanese Presentation type：Oral presentation (invited, special)

Venue：名古屋
In vitro spheroid culture for chondrogenic precursor ATDC5 cells provokes an initial stage of endochondral ossification

Jeonghyun Kim, Kosei Tomida, Eijiro Maeda, Taij Adachi, Takeo Matsumoto

The 4th International Symposium on Mechanobiology 2022.11.7 The Australian Society for Mechanobiology (AuSMB)

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Event date： 2022.11

Language：English

Venue：Sydney Country：Australia
Induction of osteocyte differentiation for mesenchymal stem cells in 3D scaffold-free spheroid culture

Jeonghyun Kim, Taiji Adachi, Takeo Matsumoto

Tissue Engineering and Regenerative Medicine International Society – Asia-Pacific Conference 2022 (TERMIS-AP 2022) 2022.10.7

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Event date： 2022.10

Language：English Presentation type：Oral presentation (general)

Venue：Jeju Country：Korea, Republic of
Differentiation fate of mesenchymal stem cells toward osteocyte is determined by actin balancing International conference

Jeonghyun Kim, Taiji Adachi

The 11th Asian-Pacific Conference on Biomechanics 2021.12.3

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Event date： 2021.12

Language：English Presentation type：Poster presentation

Venue：Kyoto, Japan
Pre-osteoblasts in three-dimensional spheroids exert osteocyte-likeness International conference

Jeonghyun Kim, Taiji Adachi

The 26th Congress of the European Society of Biomechanics 2021.7.13 European Society of Biomechanics

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Event date： 2021.7

Language：English Presentation type：Oral presentation (general)

Country：Italy
Intracellular tension of osteoblast in collagen gel elicits osteocyte alignment under uniaxially-fixed boundary condition Invited International conference

Jeonghyun Kim, Keiichi Ishikawa, Junko Sunaga, Taiji Adachi

The 2nd Joint Meeting of the European Society for Clinical Hemorheology and Microcirculation, the International Society for Clinical Hemorheology, and the International Society of Biorheology (ESCHM-ISCH-ISB 2021) 2021.7.7 The 2nd Joint Meeting of the European Society for Clinical Hemorheology and Microcirculation, the International Society for Clinical Hemorheology, and the International Society of Biorheology (ESCHM-ISCH-ISB 2021)

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Event date： 2021.7

Language：English Presentation type：Oral presentation (invited, special)

Country：Japan
Hydrostatic pressure-responsive 3D osteocytic culture model

キム・ジョンヒョン, 新岡琴音, 前田英次郎, 松本健郎

2023年度日本生体医工学会東海支部大会 2023.10.28

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Event date： 2023.10
Application of spheroid culture to mimic the initial stage of endochondral ossification process

キム・ジョンヒョン, 富田航世, 前田英次郎, 安達泰治, 松本健郎

日本機械学会 2023年度年次大会 2023.9.6

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Event date： 2023.9

Language：Japanese
Chemical osteogenesis supplements modulated the multicellular behaviors of osteocytic spheroids

2023.3.23

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Event date： 2023.3

Language：Japanese Presentation type：Oral presentation (general)

Country：Japan
Generation of tight actin filaments in response to osteogenesis supplements modulated the mechanical behaviors of osteocytic spheroids

キム・ジョンヒョン , 安達泰治 , 松本健郎

日本機械学会 2022年度年次大会 2022.9.13

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Event date： 2022.9

Language：Japanese Presentation type：Oral presentation (general)
Modulation of mechanical environment to induce osteocyte differentiation of mesenchymal stem cells International conference

Jeonghyun Kim, Takashi Inagaki, Taiji Adachi, Takeo Matsumoto

9th World Congress of Biomechanics 2022.7.12 The World Congress of Biomechanics

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Event date： 2022.7

Language：English Presentation type：Oral presentation (general)

Venue：Taiwan Country：Taiwan, Province of China
Effect of chemical osteogenesis induction on mechanical behavior of 3D osteocytic spheroids

キム・ジョンヒョン, 安達泰治, 松本健郎

第61回日本生体医工学会大会 2022.6.28 日本生体医工学会

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Event date： 2022.6

Language：Japanese Presentation type：Oral presentation (general)

Venue：新潟 Country：Japan
骨細胞スフェロイドの機械的挙動に対する骨分化誘導剤の影響

キム・ジョンヒョン, 安達泰治, 松本健郎

第45回日本バイオレオロジー学会年会 2022.6.4 日本バイオレオロジー学会

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Event date： 2022.6

Language：Japanese Presentation type：Oral presentation (general)

Venue：神奈川 Country：Japan
３次元スフェロイド培養による骨細胞分化誘導の研究

Jeonghyun Kim, Taiji Adachi

第60回日本生体医工学会大会 2021.6.16 日本生体医工学会

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Language：Japanese Presentation type：Oral presentation (general)

Country：Japan
骨芽細胞様細胞を用いた三次元構造体スフェロイドの骨細胞分化モデルへの応用

Jeonghyun Kim, Taiji Adachi

第33回バイオエンジニアリング講演会 2021.6.26 日本機械学会

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Language：Japanese Presentation type：Poster presentation

Country：Japan
三次元骨細胞組織モデルを用いた細胞バイオメカニクス

Jeonghyun Kim, Taiji Adachi

日本機械学会2021年度年次大会 2021.9.7 日本機械学会

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Language：Japanese Presentation type：Oral presentation (general)

Country：Japan

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To the head of Presentations.▲

Research Project for Joint Research, Competitive Funding, etc. 6

Emerging technologies to study biomechanical and mechanobiological characterization of bone, cartilage, muscle and tendon International coauthorship

2023.9

Nagoya – Melbourne Joint Research Workshops Fund 2023

Jeonghyun Kim, Kathryn Stok

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\1252396
骨細胞の機械的シグナル伝達機構解明に向けた静水圧負荷装置の開発

2023.4 - 2024.3

シーシーアイ株式会社研究助成プログラム

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\1000000
Practical application of bone regenerative medicine: Construction of 3D pellet culture model using pluripotent stem cells

Grant number：1546 2023.3 - 2024.3

The Hitachi Global Foundation The Kurata Grants

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\1000000
骨オルガノイド構築と骨細胞分化機構の解明

Grant number：No.5 2022.9 - 2024.3

立松財団 A1.特別研究助成

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Grant type：Competitive

Grant amount：\3000000
３次元培養モデルを用いた静水圧負荷による骨細胞分化制御に関する研究

2022.8 - 2023.7

内藤科学技術振興財団研究助成金

Kim Jeonghyun

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\1000000
Development of 4D measurement technology for bone-cartilage formation dynamics using 3D tissue-engineered constructs

2021.4 - 2023.3

Grant for Biomedical Engineering Research (Encouragement of Research), the Nakatani Foundation for Advancement of Measuring Technologies in Biomedical Engineering

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\4000000

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To the head of Research Project for Joint Research, Competitive Funding, etc..▲

KAKENHI (Grants-in-Aid for Scientific Research) 5

Elucidation of osteocyte differentiation mechanism for establishment of bone organoids

Grant number：23K17193 2023.4 - 2025.3

Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

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Authorship：Principal investigator Grant type：Competitive

Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）
Morphogenesis-based Manufacturing: Construction of optimal structures based on mechanical response of cells

Grant number：21H04533 2021.4 - 2024.3

Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)

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Authorship：Coinvestigator(s) Grant type：Competitive

Grant amount：\400000
Reconstruction of three-dimensional osteochondral model by multilayered scaffold-free tissue

Grant number：20K20181 2020.4 - 2023.3

Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists

Kim Jeonghyun

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Authorship：Principal investigator

Grant amount：\4160000

In this study, we fabricated and evaluated a cartilage scaffold-free tissue (SFT), which is a three-dimensional cell assembly derived from mouse chondroprogenitor ATDC5 cells. After long-term culture without addition of chemical differentiation supplements, hypertrophy of cells within the spheroids was confirmed, and apoptosis was also observed from the center of the spheroids. This is a phenomenon observed in the initial stage of endochondral ossification, and is a result showing the structural effect of the three-dimensional model. In addition, using human mesenchymal stem cell-derived spheroids, it was clarified that the multicellular behavior in the spheroids was greatly changed by chemical differentiation supplements. Moreover, we established an optical clearing technique necessary for cell observation inside the 3D spheroids and further developed a custom-made image analysis pipeline in Python to quantitatively evaluate cell nucleus shape in the spheroids.
骨形成再現に向けた旋回培養法による骨オルガノイド大量作製及び新たな活用法構築

Grant number：19K23604 2019.8 - 2021.3

日本学術振興会研究活動スタート支援

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Authorship：Principal investigator

Grant amount：\2860000
脱細胞担体と機械的刺激を用いた子宮組織の再生と子宮着床パッチの開発

Grant number：17J07735 2017.4 - 2018.10

日本学術振興会特別研究員奨励費

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Authorship：Principal investigator

Grant amount：\1940000

To the head of KAKENHI (Grants-in-Aid for Scientific Research).▲

Teaching Experience (On-campus) 5

機械・航空宇宙工学科実験

2024
材料力学第２及び演習

2022
物理学実験

2022
物理学実験

2021
材料力学第２及び演習

2021

To the head of Teaching Experience (On-campus).▲

Social Contribution 1

令和6年度テクノサイエンスセミナー『力に応答する細胞を見よう』

Role(s)：Lecturer,　Advisor,　Organizing member,　Demonstrator

2024.4 - 2024.8

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Type：Seminar, workshop

東海地区の高校生に，名古屋大学大学院工学研究科機械システム工学専攻の研究教育について講義と実験を通した体験学習を行い，バイオメカニクス研究の楽しさと重要性を実感してもらう．同時に，参加者と大学の教員・学生との交流の場を提供する．

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