2022/11/15 更新

写真a

ニシワキ ヒロシ
西脇 寛
NISHIWAKI Hiroshi
所属
大学院医学系研究科 附属医学教育研究支援センター 先端領域支援部門 助教
大学院担当
大学院医学系研究科
学部担当
医学部 医学科
職名
助教

学位 1

  1. 博士(医学) ( 2021年3月   名古屋大学 ) 

研究分野 1

  1. ライフサイエンス / 衛生学、公衆衛生学分野:実験系を含む  / 腸内細菌、統計学、機械学習、ゲノム解析

 

論文 8

  1. Short chain fatty acids-producing and mucin-degrading intestinal bacteria predict the progression of early Parkinson's disease.

    Nishiwaki H, Ito M, Hamaguchi T, Maeda T, Kashihara K, Tsuboi Y, Ueyama J, Yoshida T, Hanada H, Takeuchi I, Katsuno M, Hirayama M, Ohno K

    NPJ Parkinson's disease   8 巻 ( 1 ) 頁: 65   2022年6月

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    記述言語:英語  

    DOI: 10.1038/s41531-022-00328-5

    PubMed

  2. Molecular Hydrogen Enhances Proliferation of Cancer Cells That Exhibit Potent Mitochondrial Unfolded Protein Response

    Hasegawa Tomoya, Ito Mikako, Hasegawa Satoru, Teranishi Masaki, Takeda Koki, Negishi Shuto, Nishiwaki Hiroshi, Takeda Jun-ichi, LeBaron Tyler W., Ohno Kinji

    INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES   23 巻 ( 5 )   2022年3月

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    記述言語:日本語   出版者・発行元:International Journal of Molecular Sciences  

    Molecular hydrogen ameliorates pathological states in a variety of human diseases, animal models, and cell models, but the effects of hydrogen on cancer have been rarely reported. In addition, the molecular mechanisms underlying the effects of hydrogen remain mostly unelucidated. We found that hydrogen enhances proliferation of four out of seven human cancer cell lines (the responders). The proliferation-promoting effects were not correlated with basal levels of cellular reactive oxygen species. Expression profiling of the seven cells showed that the responders have higher gene expression of mitochondrial electron transport chain (ETC) molecules than the non-responders. In addition, the responders have higher mitochondrial mass, higher mitochondrial superoxide, higher mitochondrial membrane potential, and higher mitochondrial spare respiratory capacity than the non-responders. In the responders, hydrogen provoked mitochondrial unfolded protein response (mtUPR). Suppression of cell proliferation by rotenone, an inhibitor of mitochondrial ETC complex I, was rescued by hydrogen in the responders. Hydrogen triggers mtUPR and induces cell proliferation in cancer cells that have high basal and spare mitochondrial ETC activities.

    DOI: 10.3390/ijms23052888

    Web of Science

    Scopus

    PubMed

  3. Altered gut microbiota in Parkinson's disease patients with motor complications

    Takahashi Kai, Nishiwaki Hiroshi, Ito Mikako, Iwaoka Kazuhiro, Takahashi Kenta, Suzuki Yoshio, Taguchi Keita, Yamahara Kanako, Tsuboi Yoshio, Kashihara Kenichi, Hirayama Masaaki, Ohno Kinji, Maeda Tetsuya

    PARKINSONISM & RELATED DISORDERS   95 巻   頁: 11 - 17   2022年2月

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    記述言語:日本語   出版者・発行元:Parkinsonism and Related Disorders  

    Introduction: Parkinson's disease (PD) is associated with gut dysbiosis. However, whether gut dysbiosis can cause motor complications is unclear. Methods: Subjects were enrolled from four independent movement disorder centers in Japan. We performed 16S ribosomal RNA gene sequence analysis of gut microbiota. Relative abundance of gut microbiota and relationships between them and clinical characteristics were statistically analyzed. Analysis of co-variance (ANCOVA) was used to assess altered gut microbiota associated with wearing-off or dyskinesia. Results: We enrolled 223 patients with PD. Wearing-off was noted in 47.5% of patients and dyskinesia in 21.9%. We detected 98 genera of bacteria. Some changes in the gut microbiota were observed in patients with PD and motor complications. After Bonferroni correction, patients with wearing-off showed decreased relative abundance of Lachnospiraceae Blautia (p < 0.0001) and increased relative abundance of Lactobacillaceae Lactobacillus (p < 0.0001), but patients with dyskinesia no longer showed significant changes in the gut microbiota. Adjustment with two models of confounding factors followed by ANCOVA revealed that age (p < 0.0001), disease duration (p = 0.01), and wearing-off (p = 0.0004) were independent risks for the decreased relative abundance of Lachnospiraceae Blautia, and wearing-off (p = 0.009) was the only independent risk factor for the increased relative abundance of Lachnospiraceae Lactobacillus. Conclusion: Relative abundance of Lachnospiraceae Blautia and Lactobacillaceae Lactobacillus was significantly decreased and increased, respectively, in the gut microbiota of PD patients with motor complications. This indicates that an altered gut microbiota is associated with the development of motor complications in patients with advanced PD.

    DOI: 10.1016/j.parkreldis.2021.12.012

    Web of Science

    Scopus

    PubMed

  4. Intestinal Collinsella may mitigate infection and exacerbation of COVID-19 by producing ursodeoxycholate

    Hirayama Masaaki, Nishiwaki Hiroshi, Hamaguchi Tomonari, Ito Mikako, Ueyama Jun, Maeda Tetsuya, Kashihara Kenichi, Tsuboi Yoshio, Ohno Kinji

    PLOS ONE   16 巻 ( 11 ) 頁: e0260451   2021年11月

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    記述言語:日本語   出版者・発行元:PLoS ONE  

    The mortality rates of COVID-19 vary widely across countries, but the underlying mechanisms remain unelucidated. We aimed at the elucidation of relationship between gut microbiota and the mortality rates of COVID-19 across countries. Raw sequencing data of 16S rRNA V3-V5 regions of gut microbiota in 953 healthy subjects in ten countries were obtained from the public database. We made a generalized linear model (GLM) to predict the COVID- 19 mortality rates using gut microbiota. GLM revealed that low genus Collinsella predicted high COVID-19 mortality rates with a markedly low p-value. Unsupervised clustering of gut microbiota in 953 subjects yielded five enterotypes. The mortality rates were increased from enterotypes 1 to 5, whereas the abundances of Collinsella were decreased from enterotypes 1 to 5 except for enterotype 2. Collinsella produces ursodeoxycholate. Ursodeoxycholate was previously reported to inhibit binding of SARS-CoV-2 to angiotensin-converting enzyme 2; suppress pro-inflammatory cytokines like TNF-α, IL-1β, IL-2, IL-4, and IL-6; have antioxidant and anti-apoptotic effects; and increase alveolar fluid clearance in acute respiratory distress syndrome. Ursodeoxycholate produced by Collinsella may prevent COVID-19 infection and ameliorate acute respiratory distress syndrome in COVID-19 by suppressing cytokine storm syndrome.

    DOI: 10.1371/journal.pone.0260451

    Web of Science

    Scopus

    PubMed

  5. Short-Chain Fatty Acid-Producing Gut Microbiota Is Decreased in Parkinson's Disease but Not in Rapid-Eye-Movement Sleep Behavior Disorder 査読有り

    Hiroshi Nishiwaki 1, Tomonari Hamaguchi 1, Mikako Ito 1, Tomohiro Ishida 2, Tetsuya Maeda 3, Kenichi Kashihara 4, Yoshio Tsuboi 5, Jun Ueyama 2, Teppei Shimamura 6, Hiroshi Mori 7, Ken Kurokawa 7, Masahisa Katsuno 8, Masaaki Hirayama 9, Kinji Ohno

    mSystems     2020年12月

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    担当区分:筆頭著者  

  6. Meta-Analysis of Gut Dysbiosis in Parkinson's Disease 査読有り

    Hiroshi Nishiwaki MD,Mikako Ito PhD,Tomohiro Ishida MS,Tomonari Hamaguchi MD, PhD,Tetsuya Maeda MD, PhD,Kenichi Kashihara MD, PhD,Yoshio Tsuboi MD, PhD,Jun Ueyama PhD,Teppei Shimamura PhD,Hiroshi Mori PhD,Ken Kurokawa PhD,Masahisa Katsuno MD, PhD,Masaaki Hirayama MD, PhD,Kinji Ohno MD, PhD

    Movement Disorders     2020年6月

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    担当区分:筆頭著者  

    DOI: https://doi.org/10.1002/mds.28119

  7. Molecular hydrogen upregulates heat shock response and collagen biosynthesis, and downregulates cell cycles: meta-analyses of gene expression profiles 査読有り

    Hiroshi Nishiwaki 1, Mikako Ito 1, Shuto Negishi 1, Sayaka Sobue 2, Masatoshi Ichihara 2, Kinji Ohno 1

    Free Radical Research     2018年3月

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    担当区分:筆頭著者  

    DOI: 10.1080/10715762.2018.1439166

  8. Mechanism of Anomalous Tunneling in Condensed Bose System 査読有り

    Yusuke Kato, Hiroshi Nishiwaki, and Akitake Fujita

    J. Phys. Soc. Jpn.     2007年11月

▼全件表示

講演・口頭発表等 5

  1. GUT MICROBIOTA PREDICTS THE PROGRESSION OF EARLY PARKINSON'S DISEASE 国際会議

    Hiroshi Nishiwaki

    Targeting Microbiota 2021  2021年10月21日 

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    開催年月日: 2021年10月

    記述言語:英語   会議種別:ポスター発表  

  2. 水素分子作用機構解明のための網羅的遺伝子発現のメタ分析

    西脇 寛

    第 7 回日本分子状水素医学生物学会大会  2017年10月29日 

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    開催年月日: 2017年10月

  3. 水素分子作用機構解明のための網羅的遺伝子発現のメタ分析

    西脇 寛

    第 6 回日本分子状水素医学生物学会大会  2016年5月26日 

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    開催年月日: 2016年5月

    記述言語:日本語  

  4. Gut microbiota in patients with Parkinson’s disease (PD) predicts disease progression better than clinical features 国際会議

    Hiroshi Nishiwaki

    The MDS Virtual Congress 2021  2021年9月 

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    記述言語:英語   会議種別:ポスター発表  

  5. パーキンソン病患者の腸内細菌叢異常のメタ分析と予後因子

    西脇 寛

    第25回腸内細菌学会学術集会 誌上開催  2020年6月 

科研費 1

  1. 腸内細菌叢を用いた機械学習によるパーキンソン病の予後予測

    研究課題/研究課題番号:22K17343  2022年4月 - 2024年3月

    科学研究費助成事業  若手研究

    西脇 寛

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    担当区分:研究代表者 

    配分額:4680000円 ( 直接経費:3600000円 、 間接経費:1080000円 )

産業財産権 1

  1. 腸内細菌叢に占めるコリンセラ属の情報を提供する方法、当該情報を用いたCOVID−19重症化予測方法およびサイトカインストーム重症化予測方

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    出願番号:2021-120143・C20210138JP#P01  出願日:2021年7月