Faculty Profiles - TORII Yuka

Papers - TORII Yuka

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特集古くて新しい予防接種～この10年で何が変わったか～ Ⅰ.総論接種に注意を要する場合と対応

鳥居ゆか, 伊藤嘉規

小児科診療 Vol. 86 ( 10 ) page： 1171 - 1176 2023.10

Association Between <i>NUDT15 R139C</i> Genotype and Neutropenia During Antiviral Therapy in Patients With Congenital Cytomegalovirus Infection

Yamaguchi, M; Iwata, KI; Fukuda, Y; Haruta, K; Suzuki, T; Torii, Y; Ito, Y; Kawada, JI

PEDIATRIC INFECTIOUS DISEASE JOURNAL Vol. 42 ( 7 ) page： E256 - E257 2023.7

Droplet Digital PCR Development for Adenovirus Load Monitoring in Children after Hematopoietic Stem Cell Transplantation Open Access

Haruta, K; Takeuchi, S; Yamaguchi, M; Horiba, K; Suzuki, T; Torii, Y; Narita, A; Muramatsu, H; Takahashi, Y; Ito, Y; Kawada, JI

JOURNAL OF MOLECULAR DIAGNOSTICS Vol. 25 ( 6 ) page： 403 - 409 2023.6

Utility of nanopore sequencing for detecting pathogens in bronchoalveolar lavage fluid from pediatric patients with respiratory failure Open Access

Yamaguchi, M; Horiba, K; Haruta, K; Takeuchi, S; Suzuki, T; Torii, Y; Kawabe, S; Wada, S; Ikeyama, T; Ito, Y; Ogi, T; Kawada, JI

JOURNAL OF CLINICAL VIROLOGY PLUS Vol. 3 ( 2 ) 2023.6

トピックス 2017～2021年に当院で後天性サイトメガロウイルス感染症と診断された超早産児の検討

白川雄一, 杉山裕一朗, 鈴木道雄, 鳥居ゆか, 伊藤嘉規, 久保田哲夫, 服部哲夫, 加藤有一

小児科 Vol. 64 ( 5 ) page： 525 - 530 2023.5

Correlation of cytomegalovirus viral load between whole blood and plasma of congenital cytomegalovirus infection under valganciclovir treatment Open Access

Torii, Y; Morioka, I; Kakei, Y; Fujioka, K; Kakimoto, Y; Takahashi, N; Yoshikawa, T; Moriuchi, H; Oka, A; Ito, Y

BMC INFECTIOUS DISEASES Vol. 23 ( 1 ) page： 31 2023.1

Time course of skin rash, computed tomography findings, and viral load in a rheumatoid arthritis patient with severe varicella pneumonia Open Access

Kobayashi, H; Takeuchi, S; Torii, Y; Ikenouchi, T; Kawada, J; Oka, K; Kato, S; Ogawa, M

IDCASES Vol. 33 page： e01866 2023

Post-exposure prophylaxis to prevent varicella in immunocompromised children Open Access

Yamaguchi, M; Tetsuka, N; Okumura, T; Haruta, K; Suzuki, T; Torii, Y; Kawada, JI; Ito, Y

INFECTION PREVENTION IN PRACTICE Vol. 4 ( 4 ) page： 100242 2022.12

Performance of Nanopore and Illumina Metagenomic Sequencing for Pathogen Detection and Transcriptome Analysis in Infantile Central Nervous System Infections Open Access

Horiba, K; Torii, Y; Aizawa, Y; Yamaguchi, M; Haruta, K; Okumura, T; Suzuki, T; Kawano, Y; Kawada, JI; Hara, S; Saitoh, A; Giske, CG; Ogi, T; Ito, Y

OPEN FORUM INFECTIOUS DISEASES Vol. 9 ( 10 ) page： ofac504 2022.10

Quantitative assessment of viral load in the blood and urine of patients with congenital cytomegalovirus infection using droplet digital PCR Open Access

Yamaguchi, M; Kawada, J; Torii, Y; Haruta, K; Suzuki, T; Horiba, K; Takahashi, Y; Ito, Y

JOURNAL OF MEDICAL VIROLOGY Vol. 94 ( 9 ) page： 4559 - 4564 2022.9

Detection of antiviral drug resistance in patients with congenital cytomegalovirus infection using long-read sequencing: a retrospective observational study Open Access

Torii, Y; Horiba, K; Kawada, J; Haruta, K; Yamaguchi, M; Suzuki, T; Uryu, H; Kashiwa, N; Goishi, K; Ogi, T; Ito, Y

BMC INFECTIOUS DISEASES Vol. 22 ( 1 ) page： 568 2022.6

MicroRNA expression profiling of cerebrospinal fluid/serum exosomes in children with human herpesvirus 6-associated encephalitis/encephalopathy by high-throughput sequencing

Torii, Y; Kawada, J; Horiba, K; Okumura, T; Suzuki, T; Ito, Y

JOURNAL OF NEUROVIROLOGY Vol. 28 ( 1 ) page： 151 - 157 2022.2

Next-Generation Sequencing to Detect Pathogens in Pediatric Febrile Neutropenia: A Single-Center Retrospective Study of 112 Cases Open Access

Kazuhiro Horiba, Yuka Torii, Toshihiko Okumura, Suguru Takeuchi, Takako Suzuki, Jun-ichi Kawada, Hideki Muramatsu, Yoshiyuki Takahashi, Tomoo Ogi, Yoshinori Ito

Open Forum Infectious Diseases Vol. 8 ( 11 ) page： ofab223 2021.11

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：Oxford University Press (OUP)

<title>Abstract</title>
<sec>
<title>Background</title>
Febrile neutropenia (FN) is a frequent complication in immunocompromised patients. However, causative microorganisms are detected in only 10% of patients. This study aimed to detect the microorganisms that cause FN using next-generation sequencing (NGS) to idenjpgy the genome derived from pathogenic microorganisms in the bloodstream. Here, we implemented a metagenomic approach to comprehensively analyze microorganisms present in clinical samples from patients with FN.

</sec>
<sec>
<title>Methods</title>
FN is defined as 1) a neutrophil count &lt; 500/µL, and 2) fever ≥ 37.5 °C. Plasma/serum samples of 112 pediatric patients with FN, 10 patients with neutropenia without fever (NE), were sequenced by NGS and analyzed by a metagenomic pipeline PATHDET.

</sec>
<sec>
<title>Results</title>
The putative pathogens were detected by NGS in 5 of 10 patients with FN with positive for blood culture results, 15 of 87 patients (17%) with negative for blood culture results, and 3 of 8 patients with NE. Several bacteria that were common in the oral, skin, and gut flora were commonly detected in blood samples, suggesting translocation of the human microbiota to the bloodstream in the setting of neutropenia. The cluster analysis of the microbiota in blood samples using NGS demonstrated that the representative bacteria of each cluster was mostly consistent with the pathogens in each patient.

</sec>
<sec>
<title>Conclusions</title>
NGS technique has a great potential for detecting causative pathogens in patients with FN. Cluster analysis, which extracts characteristic microorganisms from a complex microbial population, may be effective to detect pathogens in minute quantities of microbiota, such as those from the bloodstream.

</sec>

DOI： 10.1093/ofid/ofab223

A Case of Descending Necrotizing Mediastinitis in a Previously Healthy Child Open Access

Okumura, T; Tetsuka, N; Yamaguchi, M; Suzuki, T; Torii, Y; Kawada, J; Ito, Y

CASE REPORTS IN PEDIATRICS Vol. 2021 page： 3159092 2021.9

Pediatric sepsis cases diagnosed with group B streptococcal meningitis using next-generation sequencing: a report of two cases. International journal Open Access

Kazuhiro Horiba, Michio Suzuki, Nobuyuki Tetsuka, Yoshihiko Kawano, Makoto Yamaguchi, Toshihiko Okumura, Takako Suzuki, Yuka Torii, Jun-Ichi Kawada, Makoto Morita, Shinya Hara, Tomoo Ogi, Yoshinori Ito

BMC infectious diseases Vol. 21 ( 1 ) page： 531 - 531 2021.6

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

BACKGROUND: Group B Streptococcus (GBS) is an important cause of invasive infection in neonates and infants. Cerebrospinal fluid (CSF) findings and culture may not show evidence of infection early in GBS meningitis. Next-generation sequencing (NGS) has the potential to detect microbial genetic material in patients with infectious diseases. We report two cases of infantile sepsis of GBS meningitis with negative results for CSF culture tests, but positive results for NGS analysis. CASE PRESENTATION: Patient 1 was a 22-day-old male infant diagnosed with sepsis and meningitis. His CSF findings showed pleocytosis, decreased glucose, and increased protein levels. However, CSF and blood culture results at admission were negative. He received a total of 3 weeks of treatment with ampicillin and cefotaxime, and showed clinical improvement. GBS was detected through NGS analysis of CSF collected at admission. Patient 2 was a 51-day-old male infant with sepsis. CSF findings on admission were normal, and blood and CSF cultures were also negative. Intravenous ampicillin and cefotaxime treatment were initiated. Treatment was de-escalated to ampicillin alone because Enterococcus faecalis was cultured from urine. He was discharged after a total of 1 week of antibiotic treatment. Six days after discharge, he was re-hospitalized for sepsis. Blood and CSF cultures were negative, and E. faecalis was again cultured from urine. He received a total of 3 weeks of ampicillin treatment for enterococcal-induced nephritis and did not relapse thereafter. NGS pathogen searches were retrospectively performed on both blood and CSF collected at the first and second admission. GBS was detected in the CSF collected at the first admission, but no significant pathogen was detected in the other samples. Inadequate treatment for GBS meningitis at the first admission may have caused the recurrence of the disease. CONCLUSION: Infantile sepsis may present bacterial meningitis that is not diagnosed by either culture testing or CSF findings. NGS analysis for CSF may be useful for confirming the diagnosis of bacterial meningitis.

DOI： 10.1186/s12879-021-06231-3

MRI findings in children with congenital cytomegalovirus infection retrospectively diagnosed with dried umbilical cord. International journal

Hiroyuki Kidokoro, Anna Shiraki, Yuka Torii, Masaharu Tanaka, Hiroyuki Yamamoto, Hirokazu Kurahashi, Koichi Maruyama, Akihisa Okumura, Jun Natsume, Yoshinori Ito

Neuroradiology Vol. 63 ( 5 ) page： 761 - 768 2021.5

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

PURPOSE: Brain MRI provides important information about suspected congenital CMV infection in neonatally underdiagnosed children. This study aimed to describe MRI findings in children in whom congenital CMV infection was not suspected during the neonatal period and was proven retrospectively. METHODS: We enrolled 31 children referred to the pediatric neurology clinic with neurological symptoms who were proven to have congenital CMV infection based on dried umbilical cord samples. Upon diagnosis, MR and CT images were assessed using the van der Knaap scoring system integrated with additional variables. Two investigators independently assessed all images. RESULTS: The age at diagnosis was < 12 months in 14, 12-24 months in 11, and > 24 months in 6 patients. The initial symptom triggering clinic referral was delayed development in 22, seizure in 5, deafness in 3, and hemiplegia in 1 patient. Of the 31 children, 30 had a white matter (WM) abnormality predominant in the deep WM of the parietal lobe (n = 25). Anterior temporal lesions were observed in 21 children. Cortical lesions were observed in 7 children, suggestive of polymicrogyria. No child had cerebellar or brainstem abnormalities. Brain CT was performed in 22 of 31 children, and 11 showed punctate cerebral calcification in the periventricular and/or deep WM. CONCLUSION: Patients with congenital CMV infection with delayed neurological symptoms show a relatively uniform pattern of parietal-dominant multifocal WM lesions and anterior temporal lesions, with or without polymicrogyria.

DOI： 10.1007/s00234-020-02603-9

Temporal dynamics of the plasma microbiome in recipients at early post-liver transplantation: a retrospective study Open Access

Okumura, T; Horiba, K; Kamei, H; Takeuchi, S; Suzuki, T; Torii, Y; Kawada, J; Takahashi, Y; Ogura, Y; Ogi, T; Ito, Y

BMC MICROBIOLOGY Vol. 21 ( 1 ) page： 104 2021.4

Authorship：Lead author Language：English Publisher：BMC Microbiology

Background: Immunosuppression during liver transplantation (LT) enables the prevention and treatment of organ rejection but poses a risk for severe infectious diseases. Immune modulation and antimicrobials affect the plasma microbiome. Thus, determining the impact of immunosuppression on the microbiome may be important to understand immunocompetence, elucidate the source of infection, and predict the risk of infection in LT recipients. We characterized the plasma microbiome of LT recipients at early post-LT and assessed the association between the microbiome and clinical events. Results: In this study, 51 patients who received LT at Nagoya University Hospital from 2016 to 2018 were enrolled. Plasma samples were retrospectively collected at the following time points: 1) within a week after LT; 2) 4 ± 1 weeks after LT; 3) 8 ± 1 weeks after LT; and 4) within 2 days after a positive blood culture. A total of 111 plasma samples were analyzed using shotgun next-generation sequencing (NGS) with the PATHDET pipeline. Relative abundance of Anelloviridae, Nocardiaceae, Microbacteriaceae, and Enterobacteriaceae significantly changed during the postoperative period. Microbiome diversity was higher within a week after LT than that at 8 weeks after LT. Antimicrobials were significantly associated with the microbiome of LT recipients. In addition, the proportion of Enterobacteriaceae was significantly increased and the plasma microbiome diversity was significantly lower in patients with acute cellular rejection (ACR) than non-ACR patients. Sequencing reads of bacteria isolated from blood cultures were predominantly identified by NGS in 8 of 16 samples, and human herpesvirus 6 was detected as a causative pathogen in one recipient with severe clinical condition. Conclusions: The metagenomic NGS technique has great potential in revealing the plasma microbiome and is useful as a comprehensive diagnostic procedure in clinical settings. Temporal dynamics of specific microorganisms may be used as indirect markers for the determination of immunocompetence and ACR in LT recipients.

DOI： 10.1186/s12866-021-02154-w

Immune cell infiltration landscapes in pediatric acute myocarditis analyzed by CIBERSORT Open Access

Kawada, J; Takeuchi, S; Imai, H; Okumura, T; Horiba, K; Suzuki, T; Torii, Y; Yasuda, K; Imanaka-Yoshida, K; Ito, Y

JOURNAL OF CARDIOLOGY Vol. 77 ( 2 ) page： 174 - 178 2021.2

Authorship：Lead author Language：English Publisher：Journal of Cardiology

Background: Myocarditis is an inflammatory disease of the myocardium, which leads to cardiac dysfunction and heart failure. Previous studies have suggested that complex cross-talk between innate and adaptive immune responses is involved in the pathogenesis of acute myocarditis. Immunohistochemistry is the current standard method for the evaluation of infiltrating immune cells, however, it is difficult to investigate and quantify many immune cell populations using this technique. Methods: Endomyocardial biopsy samples of five pediatric patients with myocarditis were analyzed by cell-type identification by estimating relative subsets of RNA transcript (CIBERSORT), a computational method for quantifying cell fractions from tissue gene expression profiles. CIBERSORT results were then compared with immunohistochemistry analyses. Results: Significant results of immune infiltrate deconvolution were obtained in four patients with fulminant myocarditis by CIBERSORT analysis. Among 22 immune cell types, 19 cell types were detected in one or more patients. Activated NK cells were the most prevalent population in two patients, whereas activated memory CD4+ T cells and M2 macrophages were the most prevalent population in one patient each. Overall CIBERSORT results were consistent with those of immunohistochemistry, although some discrepancies were observed. Conclusions: Infiltrating immune cell subsets detected by CIBERSORT analysis can reflect the time course of innate and adaptive immune responses in acute myocarditis. CIBERSORT may have the potential to characterize the detail of infiltrating immune cells in myocardial tissues and provide novel insights into the pathogenesis of acute myocarditis.

DOI： 10.1016/j.jjcc.2020.08.004

Web of Science

Scopus

PubMed

Viral Reactivations in Children with Aplastic Anemia Undergoing Allogeneic Hematopoietic Stem Cell Transplantation

Narita, A; Ito, Y; Kawashima, N; Okuno, Y; Muramatsu, H; Nishio, N; Torii, Y; Kawada, J; Kojima, S; Takahashi, Y

PEDIATRIC BLOOD & CANCER Vol. 67 2020.12

Comprehensive Detection of Candidate Pathogens in the Lower Respiratory Tract of Pediatric Patients With Unexpected Cardiopulmonary Deterioration Using Next-Generation Sequencing. International journal Open Access

Suguru Takeuchi, Jun-Ichi Kawada, Kazuhiro Horiba, Makoto Yamaguchi, Toshihiko Okumura, Takako Suzuki, Yuka Torii, Shinji Kawabe, Sho Wada, Takanari Ikeyama, Yoshinori Ito

Pediatric critical care medicine : a journal of the Society of Critical Care Medicine and the World Federation of Pediatric Intensive and Critical Care Societies Vol. 21 ( 11 ) page： E1026 - E1030 2020.11

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

OBJECTIVES: Next-generation sequencing has been applied to the investigation of microorganisms in several clinical settings. We investigated the infectious etiologies in respiratory specimens from pediatric patients with unexpected cardiopulmonary deterioration using next-generation sequencing. DESIGN: Retrospective, single-center, observational study. SETTING: Tertiary care, a children's hospital. SUBJECTS: The study enrolled a total of 16 pediatric patients with unexpected cardiopulmonary deterioration who were admitted to the PICU. Ten bronchoalveolar lavage fluid and six transtracheal aspirate samples were analyzed. INTERVENTIONS: None. MEASUREMENTS AND MAIN RESULTS: RNA libraries were prepared from specimens and analyzed using next-generation sequencing. One or more bacterial/viral pathogens were detected in the bronchoalveolar lavage fluid or transtracheal aspirate specimens from 10 patients. Bacterial and viral coinfection was considered in four cases. Compared with the conventional culture and viral antigen test results, an additional six bacterial and four viral pathogens were identified by next-generation sequencing. Conversely, among 18 pathogens identified by the conventional methods, nine pathogens were detected by next-generation sequencing. Candidate pathogens (e.g., coxsackievirus A6 and Chlamydia trachomatis) were detected by next-generation sequencing in four of 10 patients in whom no causative pathogen had been identified by conventional methods. CONCLUSIONS: Our results suggest that viral and bacterial infections are common triggers in unexpected cardiopulmonary deterioration in pediatric patients. Next-generation sequencing has the potential to contribute to clarification of the etiology of pediatric critical illness.

DOI： 10.1097/PCC.0000000000002558

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Papers - TORII Yuka