Updated on 2024/04/02

写真a

 
HAMAJIMA Rina
 
Organization
Graduate School of Bioagricultural Sciences Department of Animal Sciences Assistant Professor
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Bioresource Sciences
Title
Assistant Professor
External link

Degree 1

  1. 博士 (農学) ( 2016.3   名古屋大学 ) 

Research Interests 5

  1. Baculovirus

  2. Insect cells

  3. Anti-viral responses

  4. Siokworm

  5. リボソーム

Research Areas 3

  1. Environmental Science/Agriculture Science / Insect science

  2. Life Science / Molecular biology

  3. Life Science / Virology

Current Research Project and SDGs 1

  1. 昆虫ウイルスと昆虫が繰り広げる攻防の分子機構の解明と利用

Research History 5

  1. Nagoya University   Graduate School of Bioagricultural Sciences   Assistant Professor

    2020.10

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  2. Osaka University   Research Institute for Microbial Diseases   Researcher

    2019.7 - 2020.9

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  3. Kyoto University   Researcher

    2017.4 - 2019.6

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  4. Nagoya University   Graduate School of Bioagricultural Sciences   Researcher

    2016.4 - 2017.3

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  5. Nagoya University   Graduate School of Bioagricultural Sciences

    2015.4 - 2016.3

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Education 3

  1. Ph.D. Graduate School of Bioagricultural Sciences, Nagoya University

    2014.4 - 2016.3

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  2. Nagoya University

    2012.4 - 2014.3

  3. Nagoya University

    2008.4 - 2012.3

Professional Memberships 4

  1. 日本蚕糸学会

    2011.4

  2. 日本応用動物昆虫学会

    2014.4

  3. The Japanese Society for Virology

    2019.10

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  4. 昆虫病理研究会

    2011.4

Committee Memberships 1

  1. 日本蚕糸学会   若手の会運営委員  

    2021.4   

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    Committee type:Academic society

Awards 4

  1. Award for Excellent Poster Prize (The 69th Annual Meeting of the Japanese Society for Virology)

    2022.11  

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  2. Encouragement Award of the Japanese Society of Sericultural Science

    2015.9   Japanese Society of Sericultural Science  

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  3. Outstanding Young Scholar Award

    2015.4   The 4th Asia-Pacific Congress of Sericulture and Insect Biotechnology  

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  4. Award for Excellent Poster Prize (The 11th Symposium of Japanese Society for Insect Pathology)

    2014.9  

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Papers 22

  1. The DEAD/H-box helicase DHX9 contributes to suppression of Bombyx mori nucleopolyhedrovirus propagation in B. mori cells. Reviewed International journal

    Nao Kudome, Aika Ito, Ayaka Ota, Michihiro Kobayashi, Motoko Ikeda, Rina Hamajima

    Developmental and comparative immunology   Vol. 147   page: 104897 - 104897   2023.10

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Antiviral immune responses are mainly triggered through the recognition of virus-derived nucleic acids by host-specific pattern recognition receptors (PRRs). Here, we identified and characterized homologs of human PRRs for virus-derived DNA in Bombyx mori upon infection with a nucleopolyhedrovirus (NPV), a member of the family Baculoviridae. We found that progeny virus production of B. mori NPV was promoted in B. mori cells silenced with B. mori homolog of DEAD/H box polypeptide 9 gene (Bm-DHX9), but not in cells silenced with the other examined genes. Silencing of Bm-DHX9 expression has no effect on apoptosis induction, one of the major antiviral responses in B. mori cells. We also showed that Bm-DHX9 has the ability to bind DNA containing unmethylated C-phosphate-G-motif, which are characteristic of microbial pathogens and contained in the NPV genome with high frequency. Our findings suggest that Bm-DHX9 has the potential for sensing NPV-derived DNA to induce antiviral immune responses.

    DOI: 10.1016/j.dci.2023.104897

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  2. Identification of the minimal AcMNPV P143 protein region responsible for triggering apoptosis and rRNA degradation of Bombyx mori cells. Reviewed International journal

    Hamajima, R., Ota, A., Makino, S., Millado, J.B.H., Kobayashi, M., Ikeda, M.

    Virus research   Vol. 276   page: 1 - 9   2020.1

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.virusres.2019.197832

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  3. Antiviral immune responses of Bombyx mori cells during abortive infection with Autographa californica multiple nucleopolyhedrovirus. Reviewed International journal

    Hamajima, R., Saito, A., Makino, S., Kobayashi, M., Ikeda, M.

    Virus research   Vol. 258   page: 28 - 38   2018.10

  4. Bombyx mori homolog of tumor suppressor p53 is involved in apoptosis-mediated antiviral immunity of B. mori cells infected with nucleopolyhedrovirus Reviewed International journal

    Makino, S., Hamajima, R., Saito, A., Tomizaki, M., Iwamoto, A., Kobayashi, M., Yamada, H., Ikeda, M.

    Developmental and Comparative Immunology   Vol. 84   page: 133 - 141   2018.7

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier Ltd  

    Apoptosis is important in antiviral immunity and affects viral multiplication and pathogenesis. Here, we showed that Bombyx mori cells transiently expressing B. mori homolog of the tumor suppressor p53 (Bm-p53) protein underwent apoptosis accompanied by elevated caspase-3-like protease activity and processing of B. mori Dronc (Bm-Dronc). RNAi-mediated silencing of bm-p53 expression, which significantly diminished accumulation of bm-p53 transcript and Bm-p53 protein, prevented apoptosis of B. mori cells infected with a recombinant B. mori nucleopolyhedrovirus defective in the anti-apoptotic p35 gene (vBmΔp35) and abolished the activation of caspase-3-like protease and processing of Bm-Dronc. Apoptosis in vBmΔp35-infected B. mori cells is associated with viral DNA replication, suggesting involvement of the DNA damage response. The Bm-p53 pro-apoptotic function is also found in Spodoptera frugiperda and Lymantria dispar cells. These results indicate that apoptosis induction in vBmΔp35-infected B. mori cells is a Bm-p53-mediated process promoted by the commencement of viral DNA replication.

    DOI: 10.1016/j.dci.2018.02.009

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  5. HCF-1 encoded by baculovirus AcMNPV is required for productive nucleopolyhedrovirus infection of non-permissive Tn368 cells Reviewed International journal

    Tachibana, A., Hamajima, R., Tomizaki, M., Kondo, T., Nanba, Y., Kobayashi, M., Yamada, H., Ikeda, M.

    SCIENTIFIC REPORTS   Vol. 7 ( 1 ) page: 3807   2017.6

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:NATURE PUBLISHING GROUP  

    Baculovirus Autographa californica multiple nucleopolyhedrovirus (AcMNPV) replicates in both Spodoptera frugiperda Sf21 and Trichoplusia ni Tn368 cells, whereas AcMNPV defective in hcf-1 (host cell-factor 1) gene productively infects only Sf21 cells, indicating that HCF-1 is indispensable for the AcMNPV productive infection of Tn368 cells. Here, we demonstrated that HCF-1 protein transiently expressed in Tn368 cells promotes the DNA synthesis of Hyphantria cunea MNPV (HycuMNPV), Orygia pseudotsugata MNPV and Bombyx mori NPV, which are normally unable to replicate in Tn368 cells. We also demonstrated that a recombinant HycuMNPV harboring the hcf-1 gene successfully replicates in Tn368 cells, generating substantial yields of progeny viruses and polyhedra. These results indicate that HCF-1 encoded by AcMNPV is an essential viral factor for productive NPV infection of Tn368 cells. Taken together with the previous findings on HRF-1 (host range factor 1), the present results provide strong evidence that viral genes acquired through horizontal gene transfer play an important role in baculovirus evolution, serving to expand the host range of baculoviruses.

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  6. P143 proteins from heterologous nucleopolyhedroviruses induce apoptosis in BM-N cells derived from the silkworm Bombyx mori Reviewed International journal

    Hamajima, R., Kobayashi, M., Ikeda, M.

    VIRUS RESEARCH   Vol. 233   page: 70 - 76   2017.4

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    We previously demonstrated that ribosomal RNA (rRNA) of Bombyx mon BM-N cells is rapidly degraded upon infection with heterologous nucleopolyhedroviruses (NPVs), including Autographa californica multiple NPV (AcMNPV), Hyphantria cunea MNPV, Spodoptera exigua MNPV and S. litura MNPV, and that this response is triggered by viral P143 proteins. The transient expression of P143 proteins from heterologous NPVs was also shown to induce apoptosis and caspase-3-like protease activation in BM-N cells. In the present study, we conducted a transient expression assay using BM-N cells expressing mutant AcMNPV P143 (Ac-P143) proteins and demonstrated that five amino acid residues cooperatively participate in Ac-P143 protein-triggered apoptosis of BM-N cells. Notably, these five residues were previously shown to be required for triggering rRNA degradation in BM-N cells. As rRNA degradation in BM-N cells does not result from apoptosis, the present results suggest that Ac-P143-triggered rRNA degradation is the upstream signal for apoptosis induction in BM-N cells. We further showed that P143 protein-triggered apoptosis does not occur in S. frugiperda Sf9 or Lymantria dispar Ld652Y cells, indicating that apoptosis induction by heterologous P143 proteins is a BM-N cell-specific response. In addition, the observed induction of apoptosis in BM-N cells was found to be mediated by activation of the initiator caspase Bm-Dronc. Taken together, these results suggest that BM-N cells evolved a unique antiviral system that recognizes heterologous NPV P143 proteins to induce rRNA degradation and caspase-dependent apoptosis. (C) 2017 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.virusres.2017.03.012

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  7. Functional analysis of inhibitor of apoptosis 1 of the silkworm Bombyx mori Reviewed International journal

    Hamajima, R., Iwamoto, A., Tomizaki, M., Suganuma, I., Kitaguchi, K., Kobayashi, M., Yamada, H., Ikeda, M.

    Insect Biochemistry and Molecular Biology   Vol. 79   page: 97 - 107   2016.12

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    Recent advances in genome-wide surveys have revealed a number of lepidopteran insect homologs of mammalian and Drosophila genes that are responsible for apoptosis regulation. However, the underlying molecular mechanisms for apoptosis regulation in lepidopteran insect cells remain poorly understood. In the present study, we demonstrated that the transfection of Bombyx mori BM-N cells with dsRNA against the B. mori cellular iap1 gene (cbm-iap1) induces severe apoptosis that is accompanied by an increase of caspase-3-like protease activity. In these apoptotic cells, the cleaved form of the endogenous initiator caspase Dronc (Bm-Dronc) was detected, indicating that cBm-IAP1 protein depletion by RNAi silencing resulted in the activation of Bm-Dronc. In transient expression assays in BM-N cells, cBm-IAP1 suppressed the apoptosis triggered by Bm-Dronc overexpression and depressed the elevation of caspase-3-like protease activity, but also increased the cleaved form of Bm-Dronc protein. cBm-IAP1 also suppressed the caspase-3-like protease activity stimulated by Bm-caspase-1 overexpression. Co-immunoprecipitation experiments demonstrated that cBm-IAP1 strongly interacts with Bm-Dronc, but only has weak affinity for Bm-caspase-1. Transient expression analyses showed that truncated cBm-IAP1 proteins defective in the BIR1, BIR2 or RING domain were unable to suppress Bm-Dronc-induced apoptosis. In addition, BM-N cells expressing truncated cBm-IAP1 proteins underwent apoptosis, suggesting that intact cBm-IAP1, which has anti-apoptotic activity, was replaced or displaced by the over expressed truncated cBm-IAP1 proteins, which are incapable of interfering with the apoptotic caspase cascade. Taken together, the present results demonstrate that cBm-IAP1 is a vital negative regulator of apoptosis in BM-N cells and functions by preventing the activation and/or activity of Bm-Dronc and Bm-caspase-1. (C) 2016 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.ibmb.2016.10.012

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  8. rRNA degradation in <i>Bombyx mori</i> and <i>Bombyx mandarina</i> cells infected with heterologous nucleopolyhedroviruses Reviewed International journal

    Hamajima Rina, Yasunaga-Aoki Chisa, Iwanaga Masashi, Imanishi Shigeo, Kobayashi Jun, Sasaki Kuni, Kusakabe Takahiro, Man Lee Jae, Mon Hiroaki, Kobayashi Michihiro, Ikeda Motoko

    Journal of Insect Biotechnology and Sericology   Vol. 85 ( 3 ) page: 3_073 - 3_077   2016

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    Language:English   Publisher:The Japanese Society of Sericultural Science  

    We previously found that rRNA of BM-N cells derived from the silkworm <i>Bombyx mori</i> undergoes rapid and extensive degradation through site-specific cleavage during abortive infection with nucleopolyhedroviruses (NPVs) of <i>Autographa californica</i> (AcMNPV), <i>Hyphantria cunea</i> (HycuMNPV), <i>Spodoptera exigua</i> and <i>S. litura</i>. Here, we demonstrated that rRNA degradation also occurs in Bme21 and Bm-aff3 cells, which are derived from <i>B. mori</i> embryo and fat body, respectively, during infection with AcMNPV. rRNA degradation in Bme21 cells was also observed following HycuMNPV infection, but was not detected in Bm-aff3 cells. We further showed that rRNA in a cell line derived from <i>B. mandarina</i>, an ancestor of <i>B. mori</i>, underwent degradation in response to cellular infection with AcMNPV and HycuMNPV. In contrast, no rRNA degradation was observed in a cell line derived from <i>Antheraea pernyi</i>. Taken together, these results indicate that NPV-triggered rRNA degradation represents a mechanism of innate antiviral immunity that is unique to <i>B. mori</i> and <i>B. mandarina</i> cells.<br>

    DOI: 10.11416/jibs.85.3_073

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  9. Identification of amino acid residues of AcMNPV P143 protein involved in rRNA degradation and restricted viral replication in BM-N cells from the silkworm Bombyx mori Reviewed International journal

    Hamajima, R., Kobayashi, M., Ikeda, M.

    VIROLOGY   Vol. 485   page: 244 - 251   2015.11

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    We previously demonstrated that rRNA undergoes rapid and extensive degradation in Bombyx mori BM-N cells upon infection with AcMNPV, which is triggered by AcMNPV P143 (Ac-P143) protein. Here, we showed that six amino acid residues of Ac-P143 protein, distributing between positions 514 and 599, are involved in rRNA degradation in BM-N cells. The six residues are highly conserved among P143 proteins from AcMNPV, HycuMNPV, SeMNPV and SpItMNPV, which trigger rRNA degradation in BM-N cells upon infection, but are only partially conserved in Bm-P143 protein, which does not induce rRNA degradation in BM-N cells. We also demonstrated that substitution of only two selected residues (N565S/L578F) of Bm-P143 protein with the corresponding Ac-P143 protein residues generates a mutant Bm-P143 protein that is capable of triggering rRNA degradation in BM-N cells. These results indicate that BmNPV evolved a unique P143 protein to evade the antiviral response and allow replication in B. mori cells. (C) 2015 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2015.08.008

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  10. Baculoviruses: diversity, evolution and manipulation of insects Reviewed International journal

    Ikeda, M., Hamajima, R., Kobayashi, M.

    ENTOMOLOGICAL SCIENCE   Vol. 18 ( 1 ) page: 1 - 20   2015.1

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    Baculoviruses, members of the family Baculoviridae, are large, enveloped viruses that contain a double-stranded circular DNA genome of 80-180kbp, encoding 90-180 putative proteins. These viruses are exclusively pathogenic for arthropods, particularly insects, and have been developed, or are being developed, as environmentally sound pesticides and eukaryotic vectors for foreign protein expression, surface display, gene delivery for gene therapy, vaccine production and drug screening. The baculoviruses contain a set of approximately 30 core genes that are conserved among all baculovirus genomes sequenced to date. Individual baculoviruses also contain a number of lineage- or species-specific genes that have greatly impacted the diversification and evolution of baculoviruses. In this review, we first describe the general properties and biology of baculoviruses and then focus on the baculovirus genes and mechanisms involved in the replication, spread and survival of baculoviruses within the context of their diversity, evolution and insect manipulation.

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  11. <i>p143</i>-mediated rRNA degradation in AcMNPV-infected BM-N cells is not associated with viral DNA replication Reviewed International journal

    Hamajima Rina, Kobayashi Michihiro, Ikeda Motoko

    Journal of Insect Biotechnology and Sericology   Vol. 83 ( 1 ) page: 1_019 - 1_023   2014

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    Language:English   Publisher:The Japanese Society of Sericultural Science  

    We previously showed that rRNA cleavage and degradation occur in <i>Bombyx mori</i> BM-N cells upon infection with heterologous NPVs, including <i>Autographa californica</i> multiple NPV (AcMNPV), <i>Hyphantria cunea</i> MNPV, <i>Spodoptera exigua</i> MNPV, and <i>Spodoptera litura</i> MNPV. Additionally, transient expression assay analyses suggested that viral P143s, which are baculovirus DNA helicases that are essential for viral DNA replication, are responsible for the observed rRNA cleavage and degradation. Here, we examined whether viral DNA replication is related to rRNA cleavage and degradation in AcMNPV-infected BM-N cells using an AcMNPV temperature-sensitive-mutant (ts8) defective in P143 function at non-permissive temperature. Electrophoretic analyses of total RNAs from ts8-infected BM-N cells revealed that rRNA cleavage and degradation still occurred in the absence of viral DNA replication at non-permissive temperature. In addition, transfection analysis with an <i>ac-p143</i> null AcMNPV bacmid demonstrated that the <i>p143</i> gene is responsible for rRNA cleavage and degradation in AcMNPV-infected BM-N cells. Taken together, these results indicate the possibility that the viral DNA replication-related function of P143 is not associated with rRNA cleavage and degradation in NPV-infected BM-N cells.<br>

    DOI: 10.11416/jibs.83.1_019

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    Other Link: https://jlc.jst.go.jp/DN/JALC/10041327660?from=CiNii

  12. Novel apoptosis suppressor apsup from the baculovirus Lymantria dispar multiple nucleopolyhedrovirus precludes apoptosis by preventing proteolytic processing of initiator caspase dronc Reviewed International journal

    Yamada, H., Kitaguchi, K., Hamajima, R., Kobayashi, M., Ikeda, M.

    Journal of Virology   Vol. 87 ( 23 ) page: 12925 - 12934   2013.12

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    We previously identified a novel baculovirus-encoded apoptosis suppressor, Apsup, from the baculovirus Lymantria dispar multiple nucleopolyhedrovirus (LdMNPV). Apsup inhibits the apoptosis of L. dispar Ld652Y cells triggered by infection with p35-defective Autographa californica MNPV (vAcΔp35) and exposure to actinomycin D or UV light. Here, we examined the functional role of Apsup in apoptosis regulation in insect cells. Apsup prevented apoptosis and the proteolytic processing of L. dispar initiator caspase Dronc (Ld-Dronc) in Ld652Y cells triggered by overexpression of Ld-Dronc, LdMNPV inhibitor-of-apoptosis 3 (IAP3), or Hyphantria cunea MNPV IAP1. In vAcΔp35-infected apoptotic Ld652Y cells, Apsup restricted apoptosis induction and prevented processing of endogenous Ld-Dronc. Conversely, upon RNA interference (RNAi)-mediated silencing of apsup, LdMNPV-infected Ld652Y cells, which typically support high-titer virus replication, underwent apoptosis, accompanied by the processing of endogenous Ld-Dronc. Furthermore, endogenous Ld-Dronc coimmunoprecipitated with transiently expressed Apsup, indicating that Apsup physically interacts with Ld-Dronc. Apsup prevented the apoptosis of Sf9 cells triggered by vAcΔp35 infection but did not inhibit apoptosis or activation of caspase-3-like protease in vAcΔp35-infected Drosophila melanogaster S2 cells. Apsup also inhibited the proteolytic processing of L. dispar effector caspase Ld-caspase-1 in the transient expression assay but did not physically interact with Ld-caspase-1. These results demonstrate that Apsup inhibits apoptosis in Ld652Y cells by preventing the proteolytic processing of Ld-Dronc. Together with our previous findings showing that Apsup prevents the processing of both overexpressed Ld-Dronc and Bombyx mori Dronc, these results also demonstrate that Apsup functions as an effective apoptotic suppressor in various lepidopteran, but not dipteran, insect cells. © 2013, American Society for Microbiology.

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  13. Degradation of rRNA in BM-N cells from the silkworm Bombyx mori during abortive infection with heterologous nucleopolyhedroviruses Reviewed International journal

    Hamajima, R., Ito, Y., Ichikawa, H., Mitsutake, H., Kobayashi, J., Kobayashi, M., Ikeda, M.

    JOURNAL OF GENERAL VIROLOGY   Vol. 94 ( Pt 9 ) page: 2102 - 2111   2013.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOC GENERAL MICROBIOLOGY  

    Cell lines derived from the silkworm, Bombyx mori, are only permissive for B. mori nucleopolyhedrovirus (NPV), with other NPVs generally resulting in abortive infection. Here, we demonstrate that rRNA of B. mori BM-N cells undergoes rapid degradation through site-specific cleavage upon infection with NPVs from Autographa californica (AcMNPV), Hyphantria cunea (HycuMNPV), Spodoptera exigua (SeMNPV) and Spodoptera litura (SpltMNPV). No significant decreases in cellular RNA were observed in Ld652Y, Se301, Sf9, Splm and S2 cells infected with AcMNPV or HycuMNPV, indicating the response is unique to BM-N cells. A transient expression assay using a cosmid library of the HycuMNPV genome demonstrated that HycuMNPV P143 is responsible for rRNA degradation, which was also detected in BM-N cells transfected with plasmids expressing the P143 proteins from AcMNPV, SeMNPV and SpltMNPV. These results indicate that B. mori evolved to acquire a unique antiviral immune mechanism that is activated by P143 proteins from heterologous NPVs.

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  14. Cloning and functional characterization of the Lymantria dispar initiator caspase dronc Reviewed International journal

    Kitaguchi, K., Hamajima, R., Yamada, H., Kobayashi, M., Ikeda, M.

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 436 ( 2 ) page: 331 - 337   2013.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    Ld652Y cells from the gypsy moth, Lymantria dispar, are extremely sensitive to various apoptotic stimuli, whereas BM-N cells from the silkworm, Bombyx mori, are relatively resistant to apoptotic stimuli. We previously cloned and characterized a B. mori homologue (bm-dronc) of Drosophila melanogaster dronc. In the present study, we cloned and characterized an L dispar homologue of dronc (ld-dronc) comparatively with Bm-Dronc. The open reading frame of ld-dronc consisted of 1329 bp that was predicted to encode a 443 amino-acid polypeptide with a molecular mass of 50,706 Da and 54-57% amino acid sequence identity with Dronc homologues from other lepidopteran insects identified to date. Ld-Dronc had a long prodomain, large p20 domain, and small p10 domain, and a catalytic site composed of (308)QTCRG(312), which was distinct from the sites QACRG in Bm-Dronc and QMCRG in Dronc homologues of several other lepidopteran insects. Transiently expressed Ld-Dronc underwent proteolytic processing in the lepidopteran cell lines L. dispar Ld652Y, Spodoptera frugiperda Sf9, and B. mori BM-N, and dipteran D. melanogaster S2, but only triggered apoptosis in the lepidopteran cell lines. Endogenous Ld-Dronc underwent processing in Ld652Y cells upon infection with vAc Delta p35, but not in mock-infected Ld652Y cells, supporting the involvement of Ld-Dronc in apoptosis induction. In vAc Delta p35-infected apoptotic cells, Ld-Dronc underwent proteolytic processing more rapidly and extensively than Bm-Dronc. Similar results were obtained for Ld-Dronc and Bm-Dronc expressed transiently in S2, Ld652Y, Sf9, and BM-N cells. Taken together, these findings suggest that the intrinsic properties of Dronc proteinsare responsible, at least in part, for the differing sensitivity of Ld652Y and BM-N to apoptosis induction upon NPV infection. (C) 2013 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.bbrc.2013.05.103

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  15. Baculovirus genes modulating intracellular innate antiviral immunity of lepidopteran insect cells Reviewed International journal

    Ikeda, M., Yamada, H., Hamajima, R., Kobayashi, M.

    VIROLOGY   Vol. 435 ( 1 ) page: 1 - 13   2013.1

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    Innate immunity is essential for insects to survive infectious pathogens. In baculovirus-infected lepidopteran cells, apoptosis and global protein synthesis shutdown are major mechanisms of intracellular innate immunity that inhibit viral replication. In contrast, baculoviruses have evolved diverse genes and mechanisms to counter the antiviral immunity activated in infected cells. In this review, we summarize the current knowledge of the cellular antiviral pathways and the baculovirus genes that modulate antiviral immunity. The studies highlighted illustrate a high degree of diversity in both the cellular responses against viral infections and viral responses against intracellular antiviral immunity, providing an important basis of further studies in this field. (C) 2012 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.virol.2012.10.016

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  16. Vero cell-adapted SARS-CoV-2 strain shows increased viral growth through furin-mediated efficient spike cleavage. International journal

    Shohei Minami, Tomohiro Kotaki, Yusuke Sakai, Shinya Okamura, Shiho Torii, Chikako Ono, Daisuke Motooka, Rina Hamajima, Ryotaro Nouda, Jeffery A Nurdin, Moeko Yamasaki, Yuta Kanai, Hirotaka Ebina, Yusuke Maeda, Toru Okamoto, Taro Tachibana, Yoshiharu Matsuura, Takeshi Kobayashi

    Microbiology spectrum     page: e0285923   2024.2

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    Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) utilizes several host proteases to cleave the spike (S) protein to enter host cells. SARS-CoV-2 S protein is cleaved into S1 and S2 subunits by furin, which is closely involved in the pathogenicity of SARS-CoV-2. However, the effects of the modulated protease cleavage activity due to S protein mutations on viral replication and pathogenesis remain unclear. Herein, we serially passaged two SARS-CoV-2 strains in Vero cells and characterized the cell-adapted SARS-CoV-2 strains in vitro and in vivo. The adapted strains showed high viral growth, effective S1/S2 cleavage of the S protein, and low pathogenicity compared with the wild-type strain. Furthermore, the viral growth and S1/S2 cleavage were enhanced by the combination of the Δ68-76 and H655Y mutations using recombinant SARS-CoV-2 strains generated by the circular polymerase extension reaction. The recombinant SARS-CoV-2 strain, which contained the mutation of the adapted strain, showed increased susceptibility to the furin inhibitor, suggesting that the adapted SARS-CoV-2 strain utilized furin more effectively than the wild-type strain. Pathogenicity was attenuated by infection with effectively cleaved recombinant SARS-CoV-2 strains, suggesting that the excessive cleavage of the S proteins decreases virulence. Finally, the high-growth-adapted SARS-CoV-2 strain could be used as the seed for a low-cost inactivated vaccine; immunization with this vaccine can effectively protect the host from SARS-CoV-2 variants. Our findings provide novel insights into the growth and pathogenicity of SARS-CoV-2 in the evolution of cell-cell transmission.IMPORTANCEThe efficacy of the S protein cleavage generally differs among the SARS-CoV-2 variants, resulting in distinct viral characteristics. The relationship between a mutation and the entry of SARS-CoV-2 into host cells remains unclear. In this study, we analyzed the sequence of high-growth Vero cell-adapted SARS-CoV-2 and factors determining the enhancement of the growth of the adapted virus and confirmed the characteristics of the adapted strain by analyzing the recombinant SARS-CoV-2 strain. We successfully identified mutations Δ68-76 and H655Y, which enhance viral growth and the S protein cleavage by furin. Using recombinant viruses enabled us to conduct a virus challenge experiment in vivo. The pathogenicity of SARS-CoV-2 introduced with the mutations Δ68-76, H655Y, P812L, and Q853L was attenuated in hamsters, indicating the possibility of the attenuation of excessive cleaved SARS-CoV-2. These findings provide novel insights into the infectivity and pathogenesis of SARS-CoV-2 strains, thereby significantly contributing to the field of virology.

    DOI: 10.1128/spectrum.02859-23

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  17. Ultrasensitive detection of SARS-CoV-2 nucleocapsid protein using large gold nanoparticle-enhanced surface plasmon resonance. Reviewed International journal

    Taka-Aki Yano, Taira Kajisa, Masayuki Ono, Yoshiya Miyasaka, Yuichi Hasegawa, Atsushi Saito, Kunihiro Otsuka, Ayuko Sakane, Takuya Sasaki, Koji Yasutomo, Rina Hamajima, Yuta Kanai, Takeshi Kobayashi, Yoshiharu Matsuura, Makoto Itonaga, Takeshi Yasui

    Scientific reports   Vol. 12 ( 1 ) page: 1060 - 1060   2022.1

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    The COVID-19 pandemic has created urgent demand for rapid detection of the SARS-CoV-2 coronavirus. Herein, we report highly sensitive detection of SARS-CoV-2 nucleocapsid protein (N protein) using nanoparticle-enhanced surface plasmon resonance (SPR) techniques. A crucial plasmonic role in significantly enhancing the limit of detection (LOD) is revealed for exceptionally large gold nanoparticles (AuNPs) with diameters of hundreds of nm. SPR enhanced by these large nanoparticles lowered the LOD of SARS-CoV-2 N protein to 85 fM, resulting in the highest SPR detection sensitivity ever obtained for SARS-CoV-2 N protein.

    DOI: 10.1038/s41598-022-05036-x

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  18. A reverse genetics system for human rotavirus G2P[4] Reviewed International journal

    Rina Hamajima, Tina Lusiany, Shohei Minami, Ryotaro Nouda, Jeffery A. Nurdin, Moeko Yamasaki, Nobumichi Kobayashi, Yuta Kanai, Takeshi Kobayashi

    Journal of General Virology   Vol. 103 ( 12 )   2022

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    Rotaviruses (RVs) are an important cause of acute gastroenteritis in young children. Recently, versatile plasmid-based reverse genetics systems were developed for several human RV genotypes; however, these systems have not been developed for all commonly circulating human RV genotypes. In this study, we established a reverse genetics system for G2P[4] human RV strain HN126. Nucleotide sequence analysis, including that of the terminal ends of the viral double-stranded RNA genome, revealed that HN126 possessed a DS-1-like genotype constellation. Eleven plasmids, each encoding 11 gene segments of the RV genome, and expression plasmids encoding vaccinia virus RNA capping enzyme (D1R and D12L), Nelson Bay orthoreovirus FAST, and NSP2 and NSP5 of HN126, were transfected into BHK-T7 cells, and recombinant strain HN126 was generated. Using HN126 or simian RV strain SA11 as backbone viruses, reassortant RVs carrying the outer and intermediate capsid proteins (VP4, VP7 and VP6) of HN126 and/or SA11 (in various combinations) were generated. Viral replication analysis of the single, double and triple reassortant viruses suggested that homologous combination of the VP4 and VP7 proteins contributed to efficient virus infectivity and interaction between other viral or cellular proteins. Further studies of reassortant viruses between simian and other human RV strains will contribute to developing an appropriate model for human RV research, as well as suitable backbone viruses for generation of recombinant vaccine candidates.

    DOI: 10.1099/jgv.0.001816

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  19. NISES-AnPe-428 cell line derived from the Chinese oak silkworm Antheraea pernyi is permissive for multiple nucleopolyhedrovirus species from insects of four different families. Reviewed International journal

    Isobe, S., Ota, A., Takata, S., Hamajima, R., Makino, S., Kobayashi, J., Kobayashi, M., Ikeda, M.

    Cytotechnology   Vol. 73 ( 4 ) page: 643 - 655   2021.8

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    The cell line NISES-AnPe-428 (AnPe), derived from the Chinese oak silkworm Antheraea pernyi, was characterized for its permissiveness and productivity for six different nucleopolyhedrovirus (NPV) species. These NPVs included homologous Antheraea pernyi NPV (AnpeNPV) and heterologous Autographa californica multiple NPV (AcMNPV), Bombyx mori NPV (BmNPV), Hyphantria cunea MNPV (HycuMNPV), Spodoptera exigua MNPV (SeMNPV), and Lymantria dispar MNPV (LdMNPV), representing viruses that had been isolated from insect species belonging to five different families (Saturniidae, Noctuidae, Bombycidae, Arctiidae, and Lymantriidae). We found that AnPe cells supported productive replication of AnpeNPV, AcMNPV, BmNPV, HycuMNPV, and SeMNPV to varying degrees. Upon infection with SeMNPV, a subset of AnPe cell population in the culture underwent apoptosis, while remaining cells produced limited amounts of progeny virions and polyhedra. AnPe cells were refractory to LdMNPV infection and failed to support replication of viral DNA, indicating that viral replication was restricted at or prior to the step of viral DNA replication. These results indicated that AnPe cells have the potential to provide excellent systems for studying the molecular mechanisms underlying cellular permissiveness for NPV replication and host-range determination of NPVs.

    DOI: 10.1007/s10616-021-00485-0

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  20. Combining machine learning and nanopore construction creates an artificial intelligence nanopore for coronavirus detection. Reviewed International journal

    Taniguchi, M., Minami, S., Ono, C., Hamajima, R., Morimura, A., Hamaguchi, S., Akeda, Y., Kanai, Y., Kobayashi, T., Kamitani, W., Terada, Y., Suzuki, K., Hatori, N., Yamagishi, Y., Washizu, N., Takei, H., Sakamoto, O., Naono, N., Tatematsu, K., Washio, T., Matsuura, Y., Tomono, K.

    Nature communications   Vol. 12 ( 1 ) page: 3726 - 3726   2021.6

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    High-throughput, high-accuracy detection of emerging viruses allows for the control of disease outbreaks. Currently, reverse transcription-polymerase chain reaction (RT-PCR) is currently the most-widely used technology to diagnose the presence of SARS-CoV-2. However, RT-PCR requires the extraction of viral RNA from clinical specimens to obtain high sensitivity. Here, we report a method for detecting novel coronaviruses with high sensitivity by using nanopores together with artificial intelligence, a relatively simple procedure that does not require RNA extraction. Our final platform, which we call the artificially intelligent nanopore, consists of machine learning software on a server, a portable high-speed and high-precision current measuring instrument, and scalable, cost-effective semiconducting nanopore modules. We show that artificially intelligent nanopores are successful in accurately identifying four types of coronaviruses similar in size, HCoV-229E, SARS-CoV, MERS-CoV, and SARS-CoV-2. Detection of SARS-CoV-2 in saliva specimen is achieved with a sensitivity of 90% and specificity of 96% with a 5-minute measurement.

    DOI: 10.1038/s41467-021-24001-2

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  21. Identification and characterization of Bombyx mori homologs of Bonus, Mdm2, Rad6, Sce, and Synoviolin Reviewed International journal

    Millado, J.B.H., Hamajima, R., Sugiura, W., Makino, S., Kobayashi, M., Ikeda, M.

    Journal of Insect Biotechnology and Sericology   Vol. 90 ( 2 ) page: 21 - 32   2021.6

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  22. Transient expression assay reveals kinetic difference in the proteolytic processing between Dronc proteins from the gypsy moth Lymantria dispar and the silkworm Bombyx mori Reviewed International journal

    Kitaguchi, K., Hamajima, R., Yamada, H., Kobayashi, M., Ikeda, M.

    Journal of Insect Biotechnology and Sericology   Vol. 82 ( 2 ) page: 49 - 54   2013

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    Ld652Y cells from the gypsy moth, Lymantria dispar, are highly sensitive to apoptotic stimuli compared to BM-N cells from the silkworm, Bombyx mori, and undergo apoptosis upon infection with various nucleopolyhe-droviruses (NPVs). We previously cloned and characterized Dronc homologues from L. dispar Ld652Y and B. mori BM-N cells and demonstrated that L. dispar Dronc (Ld-Dronc) undergoes proteolytic processing more rapidly and extensively than does B. mori Dronc (Bm-Dronc) upon infection with vAcΔp35, a p35-defective Autographa californica multiple NPV. Here, we comparatively examined expression and proteolytic processing of the Ld- and Bm-Dronc proteins in the transient expression assays using three different insect cell lines, Ld652Y, BM-N and Spodoptera frugiperda Sf9. We demonstrate that even in the transient expression assays, Ld-Dronc undergoes proteolytic processing and activates caspase-3-like protease more rapidly and extensively than Bm-Dronc in a cell-line independent manner. These results indicate that intrinsic properties of Dronc are involved in the differing capacity of Ld652Y and BM-N cells to induce apoptosis in response to apoptotic stimuli, including NPV infection.

    DOI: 10.11416/jibs.82.2_049

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MISC 3

  1. 昆虫の防御応答から迫るバキュロウイルスの宿主決定メカニズム—Antiviral defenses against baculovirus infection in lepidopteran insects—生物生態から制御剤まで

    浜島 りな

    日本農薬学会誌 = Japanese journal of pesticide science   Vol. 47 ( 2 ) page: 78 - 82   2022.8

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  2. RNA-seq法を用いた核多角体病ウイルス感染カイコ細胞のトランスクリプトーム解析

    浜島りな, 佐藤昌直, 小林迪弘, 池田素子

    日本蚕糸学会大会・蚕糸・昆虫機能学術講演会講演要旨集   Vol. 86th   2016

  3. 核多角体病ウイルス感染細胞における抗ウイルス応答 : 全タンパク質合成停止 (特集 昆虫の生体防御メカニズムのトピックス)

    浜島 りな, 小林 迪弘, 池田 素子

    蚕糸・昆虫バイオテック   Vol. 84 ( 3 ) page: 221 - 236   2015.12

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    Language:Japanese   Publisher:日本蚕糸学会  

    DOI: https://doi.org/10.11416/konchubiotec.84.3_221

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Presentations 102

  1. AcMNPV 感染カイコ細胞において検出されるrRNA分解断片の解析

    原屋正龍・坂上裕喜・池田素子・浜島りな

    令和6年度蚕糸・昆虫機能利用学術講演会  2024.3 

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  2. LdMNPVのアポトーシス抑制因子Apsupと相互作用する細胞因子の探索

    北谷華穂・池田素子・浜島りな

    令和6年度蚕糸・昆虫機能利用学術講演会  2024.3 

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  3. AcMNPV感染カイコ細胞におけるリボソームタンパク質の挙動の調査

    坂上裕喜・池田素子・浜島りな

    令和6年度蚕糸・昆虫機能利用学術講演会  2024.3 

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  4. AcMNPV感染カイコ細胞におけるrRNA分解の誘導を担う分子機構の解析

    原屋正龍・池田素子・浜島りな

    日本蚕糸学会中部支部第79回・東海支部第75回大会  2024.1 

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  5. AcMNPV感染カイコ細胞におけるリボソームRNA 分解断片の配列解析

    原屋正龍・池田素子・浜島りな

    第80回昆虫病理研究会  2023.12 

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  6. BmNPVの増殖抑制に関与するカイコDHX9の下流因子の探索

    久土目奈央・池田素子・浜島りな

    第80回昆虫病理研究会  2023.12 

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  7. Antiviral defenses of the silkworm cells against baculovirus infection Invited

    Hamajima Rina

    JSPS国際交流事業二国間セミナー シンポジウム Frontiers of insect virus research contributing to pesticide reduction and training the next generation researchers  2023.9 

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    Event date: 2023.9

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

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  8. サクサン培養細胞における6種の核多角体病ウイルスの感染性調査

    礒部 詩保・浜島 りな・小林 淳・ 池田 素子

    令和5年度蚕糸・昆虫機能利用学術講演会  2023.3.7 

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  9. カイコ細胞におけるrRNA分解誘導に関与する核多角体病ウイルスP143タンパク質 の立体構造予測

    浜島 りな・原屋 正龍・池田 素子

    令和5年度蚕糸・昆虫機能利用学術講演会  2023.3.7 

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  10. NPVのIE-1がカイコ細胞iap1遺伝子のmRNA量に及ぼす影響の調査

    河合 祐作・浜島 りな・池田 素子

    令和5年度蚕糸・昆虫機能利用学術講演会  2023.3.7 

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  11. AcMNPV感染カイコ細胞において認められるリボソームRNA分解断片の解析

    原屋 正龍・池田 素子・浜島 りな

    令和5年度蚕糸・昆虫機能利用学術講演会  2023.3.7 

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  12. 核多角体病ウイルスIE-1がカイコ細胞iap1遺伝子の発現に及ぼす影響の調査

    河合 祐作・浜島 りな・池田 素子

    日本蚕糸学会 中部支部第78回・東海支部第74回研究発表会  2023.1.5 

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  13. AcMNPV感染カイコ細胞におけるリボソームRNAの切断部位の同定

    原屋 正龍・池田 素子・浜島 りな

    日本蚕糸学会 中部支部第78回・東海支部第74回研究発表会  2023.1.5 

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  14. バキュロウイルス感染に対するカイコ細胞のリボソームRNA分解による防御応答 Invited

    浜島りな

    第40回KITライフサイエンスセミナー  2022.11.24 

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  15. ロタウイルス感染に関与する宿主因子Tumor Associated Calcium Signal Transducer 2の機能解析

    山﨑 萌子, 金井 祐太, 浜島 りな, 小瀧 将裕, 南 昌平, 納田 遼太郎, 小林 剛

    第69回日本ウイルス学会学術集会  2022.11.13 

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    Presentation type:Oral presentation (general)  

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  16. ヒトロタウイルスHN126株におけるリバースジェネティクス系の確立

    浜島 りな, Tina Lusiany, 南 昌平, 納田 遼太郎, Jeffery A. Nurdin, 山﨑 萌子, 小林 宣道, 金井 祐太, 小林 剛

    第69回日本ウイルス学会学術集会  2022.11.13 

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    Presentation type:Poster presentation  

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  17. BmNPV感染カイコ細胞におけるウイルスDNAセンサー相同体の機能解析

    久土目奈央, 浜島りな, 伊東愛花, 池田素子

    第79回昆虫病理研究会  2022.11.5 

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  18. AcMNPV感染に伴うカイコ細胞IAP1の減少を担う分子機構の解析

    河合祐作, 浜島りな, 池田素子

    第79回昆虫病理研究会  2022.11.5 

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  19. AcMNPV感染カイコ細胞におけるリボソームRNAの分解機構の解析

    原屋正龍, 池田素子, 浜島りな

    第79回昆虫病理研究会  2022.11.5 

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  20. カイコ細胞におけるアポトーシス誘導を担うBm-p53のドメイン解析

    杉浦和香, 浜島りな, 牧野静花, MILLADO Justine, Bennette H, 小林迪弘, 池田素子

    令和4年度蚕糸・昆虫機能利用学術講演会  2022.3.14 

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    Event date: 2022.3

    Presentation type:Oral presentation (general)  

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  21. AcMNPV感染時に誘導されるカイコ細胞IAP1の減少機構の解析

    河合祐作, 浜島りな, 小林迪弘, 池田素子

    令和4年度蚕糸・昆虫機能利用学術講演会  2022.3.14 

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  22. BmNPV感染カイコ細胞におけるカイコDHX9相同体の機能解析

    久土目奈央, 浜島りな, 小林迪弘, 池田素子

    令和4年度蚕糸・昆虫機能利用学術講演会  2022.3.14 

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  23. 昆虫の防御応答から迫るバキュロウイルスの宿主決定メカニズム Invited

    浜島りな

    日本農薬学会第 47 回大会 (共催: 第 21 回農薬バイオサイエンス研究会) 

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    Event date: 2022.3

    Presentation type:Oral presentation (invited, special)  

    Venue:オンライン  

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  24. カイコ細胞におけるアポトーシス誘導を担うBm-p53の機能解析

    杉浦和香, 浜島りな, 牧野静花, MILLADO Justine Bennette H., 小林迪弘, 池田素子

    日本蚕糸学会中部支部第77回・東海支部第73回大会 

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    Presentation type:Oral presentation (general)  

    Venue:オンライン  

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  25. AcMNPV感染カイコ細胞において誘導される細胞IAP1の減少機構の解析

    河合祐作, 浜島りな, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第77回・東海支部第73回大会 

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    Presentation type:Oral presentation (general)  

    Venue:オンライン  

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  26. BmNPV感染カイコ細胞におけるウイルスDNA認識受容体相同体の機能解析

    久土目奈央, 浜島りな, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第77回・東海支部第73回大会 

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  27. A genome-wide CRISPR-Cas9 screening approach to identify host factors for rotavirus infection

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  28. AcMNPV 感染カイコ細胞における rRNA 分解実行因子とリボソームの挙動の調査

    太田 綾香・浜島 りな・Millado, Justine Bennette H.・小林 迪弘・池田 素子

    日本蚕糸学会第91回学会  2021.3.19 

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    Venue:オンライン  

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  29. Functional analysis of bonus, MDM2, rad6, SCE, and synoviolin homologs from Bombyx mori

    Jusitine Bennette H. Millado・Rina Hamajima・Shizuka Makino・Michihiro Kobayashi・Motoko Ikeda

    日本蚕糸学会第91回学会  2021.3.19 

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  30. カイコ核多角体病ウイルスの bm-iap1 と bm-iap2 のアポトーシスにおける機能解析

    髙田 史緒里・浜島 りな・斎藤 綾・小林 迪弘・池田 素子

    日本蚕糸学会第91回学会  2021.3.19 

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  31. カイコp53タンパク質のアポトーシス誘導活性及び局在性を担うドメインの調査

    杉浦 和香・浜島 りな・牧野 静花・Millado, Justine Bennette H.・小林 迪弘・ 池田 素子

    日本蚕糸学会第91回学会  2021.3.19 

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  32. NPV 感染カイコ細胞における抗ウイルス応答の誘導に関与する DNA 認識受容体の探索

    久土目奈央・浜島りな・小林迪弘・池田素子

    日本蚕糸学会第91回学会  2021.3.19 

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  33. Cloning and characterization of Bombyx mori homologs of bonus, mdm2, rad6, SCE, and synoviolin

    Jusitine Bennette H. Millado・Rina Hamajima・Shizuka Makino・Michihiro Kobayashi・Motoko Ikeda

    日本蚕糸学会中部支部第 76 回・東海支部第 72 回大会  2021.1.8 

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    Event date: 2021.1

    Language:English   Presentation type:Oral presentation (general)  

    Venue:オンライン  

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  34. カイコ細胞におけるカイコ核多角体病ウイルスの bm-iap1 と bm-iap2 の機能解析

    髙田 史緒里・浜島 りな・斎藤 綾・小林 迪弘・池田 素子

    日本蚕糸学会中部支部第 76 回・東海支部第 72 回大会  2021.1.8 

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    Event date: 2021.1

    Presentation type:Oral presentation (general)  

    Venue:オンライン  

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  35. カイコ p53 タンパク質の局在性及びアポトーシス誘導活性を担うドメインの探索

    杉浦 和香・浜島 りな・牧野 静花・Millado, Justine Bennette H.・小林 迪弘・ 池田 素子

    日本蚕糸学会中部支部第 76 回・東海支部第 72 回大会  2021.1.8 

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    Event date: 2021.1

    Presentation type:Oral presentation (general)  

    Venue:オンライン  

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  36. rRNA 分解に関わる Ac-P143 機能領域の決定と rRNA 分解実行因子の探索

    太田 綾香・浜島 りな・Millado, Justine Bennette H.・小林 迪弘・池田 素子

    日本蚕糸学会中部支部第 76 回・東海支部第 72 回大会  2021.1.8 

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    Event date: 2021.1

    Presentation type:Oral presentation (general)  

    Venue:オンライン  

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  37. 核多角体病ウイルス感染カイコ細胞におけるカイコ STING 相同体の機能解析

    久土目 奈央・浜島 りな・小林 迪弘・池田 素子

    日本蚕糸学会中部支部第 76 回・東海支部第 72 回大会  2021.1.8 

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    Event date: 2021.1

    Presentation type:Oral presentation (general)  

    Venue:オンライン  

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  38. ロタウイルス感染における多様な細胞表面糖鎖レセプターの意義

    山﨑萌子, 金井祐太, 浜島りな, 南昌平, Pannacha Pimfhun, 納田遼太郎, Jeffry Nurdin・Tina Lusiany, 松本真依, 大西未紗, 小林剛

    第163回日本獣医学会学術集会  2020.9.1 

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    Event date: 2020.9

    Presentation type:Oral presentation (general)  

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  39. Development of rotavirus vector as vaccine platform for intestinal pathogens. International conference

    Kanai, Y., Hamajima, R., Pannacha, P., Nouda, R., Nurdin, J., Nomura, K., Lusiany, T., Yamasaki, M., Onishi, M., Kawagishi, T., Kobayashi, T.

    ASV2020: 39th Annual Meeting for the American Society for Virology (cancelled)  2020.6.13 

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    Event date: 2020.6 - 2020

    Language:English   Presentation type:Poster presentation  

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  40. AcMNPV P143のrRNA分解とアポトーシスの誘導を引き起こす最小配列の決定

    太田綾香, 浜島りな, MILLADO Justine Bennette H., 小林迪弘, 池田素子

    日本蚕糸学会中部支部第75回・東海支部第71回大会  2019.12.1 

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    Event date: 2019.12

    Presentation type:Oral presentation (general)  

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  41. NPV感染マイマイガ細胞におけるcld-iap1減少機構の解析

    下山敦志 浜島りな, 小林迪弘, 山田早人, 池田素子

    日本蚕糸学会中部支部第75回・東海支部第71回大会  2019.12.1 

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  42. カイコ細胞における抗ウイルス応答に関与するp143とep32遺伝子を欠損させたHycuMNPVバクミドの作成と感染性の調査

    奥野文人, 浜島りな, 橘亜美, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第75回・東海支部第71回大会  2019.12.1 

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    Presentation type:Oral presentation (general)  

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  43. カイコ核多角体病ウイルスがコードするbm-iap1とbm-iap2の機能解析

    髙田史緒里, 浜島りな, 牧野静花, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第75回・東海支部第71回大会  2019.12.1 

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    Event date: 2019.12

    Presentation type:Oral presentation (general)  

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  44. 核多角体病ウイルスとカイコ細胞が繰り広げる攻防の分子機構 Invited

    浜島りな

    第77回昆虫病理研究会  2019.9.21 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

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  45. Autographa californica核多角体病ウイルスP143のrRNA分解とアポトーシスの誘導に関与する領域の探索

    浜島りな, 太田綾香, MILLADO Justine, Bennette H, 小林迪弘, 池田素子

    日本蚕糸学会第89回大会  2019.3.1 

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    Event date: 2019.3

    Presentation type:Oral presentation (general)  

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  46. カイコ細胞におけるBm-p53の制御機構の解析

    牧野静花, 浜島りな, MILLADO Justine, Bennette H, 富崎萌, 小林迪弘, 池田素子

    日本蚕糸学会第89回大会  2019.3.1 

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    Event date: 2019.3

    Presentation type:Oral presentation (general)  

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  47. AcMNPV感染カイコ細胞におけるNPV の感染現象と抗ウイルス応答誘導の関係性の調査

    浜島りな, 斎藤 綾, 牧野静花, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第74回・東海支部第70回大会  2018.12.1 

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    Event date: 2018.12

    Presentation type:Oral presentation (general)  

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  48. BmNPV感染カイコ細胞におけるカイコ p53を介したアポトーシス誘導機構の解析

    牧野静花, 浜島りな, 富崎萌, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第74回・東海支部第70回大会  2018.12.1 

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    Event date: 2018.12

    Presentation type:Oral presentation (general)  

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  49. マイマイガ細胞における Ld-p53 の同定と機能解析

    下山敦志, 今泉明敏, 浜島りな, 小林迪弘, 山田早人, 池田素子

    日本蚕糸学会中部支部第74回・東海支部第70回大会  2018.12.1 

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    Event date: 2018.12

    Presentation type:Oral presentation (general)  

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  50. HycuMNPV 感染カイコ細胞が誘導する抗ウイルス応答に関与する hycu-p143 と hycu-ep32 の機能解析

    奥野文人, 浜島りな, 橘亜美, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第74回・東海支部第70回大会  2018.12.1 

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    Event date: 2018.12

    Presentation type:Oral presentation (general)  

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  51. HycuMNPV感染カイコ細胞における抗ウイルス応答誘導遺伝子p143とep32の機能解析

    奥野文人, 浜島りな, 橘亜美, 小林迪弘, 池田素子

    第13回昆虫病理研究会シンポジウム  2018.9.1 

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    Event date: 2018.9

    Presentation type:Poster presentation  

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  52. マイマイガ細胞におけるp53相同体の同定と機能解析

    下山敦志, 今泉明敏, 浜島りな, 小林迪弘, 山田早人, 池田素子

    第13回昆虫病理研究会シンポジウム  2018.9.1 

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    Event date: 2018.9

    Presentation type:Poster presentation  

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  53. NPV感染カイコ細胞のアポトーシス誘導におけるBm-p53の機能解析

    牧野静花, 浜島りな, 富崎萌, 小林迪弘, 池田素子

    第13回昆虫病理研究会シンポジウム  2018.9.1 

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    Event date: 2018.9

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  54. 機能不全リボソーム品質管理機構25S NRDにおけるプロテアソーム標的因子の探索

    浜島りな, 酒井朗恵, 藤井耕太郎, 北畠真, 大野睦人

    第5回Ribosome Meeting  2018.9.1 

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    Event date: 2018.9

    Presentation type:Poster presentation  

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  55. 機能不全25S rRNA分解機構におけるプロテアソーム標的因子の探索

    浜島りな, 酒井朗恵, 藤井耕太郎, 北畠真, 大野睦人

    第20回日本RNA学会年会  2018.7.1 

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    Event date: 2018.7

    Presentation type:Poster presentation  

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  56. 核多角体病ウイルス感染カイコ細胞のアポトーシス誘導におけるBm-P53の機能解析

    牧野静花, 浜島りな, 富崎萌, 小林迪弘, 山田早人, 池田素子

    日本蚕糸学会第88回大会  2018.3.1 

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    Event date: 2018.3

    Presentation type:Oral presentation (general)  

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  57. BmNPV感染カイコ細胞のアポトーシス誘導におけるBm-P53の機能解析

    牧野静花, 浜島りな, 富崎萌, 小林迪弘, 山田早人, 池田素子

    日本蚕糸学会中部支部第73回・東海支部第69回大会  2017.12.1 

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    Event date: 2017.12

    Presentation type:Oral presentation (general)  

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  58. カイコ細胞が誘導するrRNA分解とアポトーシスに関与する核多角体病ウイルスP143の機能解析

    浜島りな, 小林迪弘, 山田早人, 池田素子

    日本蚕糸学会第87回大会  2017.3.1 

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    Event date: 2017.3

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  59. 核多角体病ウイルス感染カイコ細胞が誘導するアポトーシスにおけるカイコのP53およびIAP antagonistの機能解析

    牧野静花, 浜島りな, 富崎萌, 小林迪弘, 山田早人, 池田素子

    日本蚕糸学会第87回大会  2017.3.1 

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    Event date: 2017.3

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  60. 遺伝子組換えHycuMNPVバクミドを用いた AcMNPV hcf-1 遺伝子の機能解析

    橘亜美, 浜島りな, 富崎萌, 近藤拓也, 小林迪弘, 池田素子

    日本蚕糸学会第87回大会  2017.3.1 

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  61. HycuMNPVバクミドを用いた hcf-1 遺伝子の機能ドメイン解析

    橘亜美, 浜島りな, 富崎萌, 近藤拓也, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第72回・東海支部第68回大会  2016.12.1 

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    Event date: 2016.12

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  62. NPV感染カイコ細胞に誘導されるアポトーシスにおけるBmP53とIAPアンタゴニストの機能解析

    牧野静花, 浜島りな, 富崎萌, 小林迪弘, 山田早人, 池田素子

    日本蚕糸学会中部支部第72回・東海支部第68回大会  2016.12.1 

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  63. 核多角体病ウイルス感染カイコ細胞が発動するリボソームRNA 分解による抗ウイルス応答 Invited

    浜島りな

    日本蚕糸学会中部支部第72回・東海支部第68回大会  2016.12.1 

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  64. AcMNPV hcf-1遺伝子のTn368細胞におけるNPV感染への影響

    橘亜美, 浜島りな, 富崎萌, 近藤拓也, 小林迪弘, 池田素子

    第12回昆虫病理研究会シンポジウム  2016.9.1 

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    Event date: 2016.9

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  65. hcf-1 gene of AcMNPV is an essential viral factor required for productive infection of HycuMNPV in Tn368 cells. International conference

    Tachibana, A, Hamajima, R, Tomizaki, M, Kondo, T, Nanba, Y, Kobayashi, M, Ikeda, M

    2016.9.1 

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    Event date: 2016.9

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  66. チョウ目昆虫細胞における核多角体病ウイルスP143タンパク質のアポトーシス誘導能の解析

    浜島りな, 小林迪弘, 山田早人, 池田素子

    第12回昆虫病理研究会シンポジウム  2016.9.1 

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  67. RNA-seq transcriptome analysis of Bombyx mori cells infected with B. mori nucleopolyhedrovirus (NPV) and Autographa californica multiple NPV International conference

    Hamajima, R, Sato, M, Kobayashi, M, Ikeda, M

    2016.9.1 

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    Language:English   Presentation type:Oral presentation (general)  

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  68. 核多角体病ウイルス感染カイコ細胞のアポトーシス誘導における P53 および IAP アンタゴニスト相同体の機能解析

    牧野静花, 浜島りな, 富崎萌, 小林迪弘, 山田早人, 池田素子

    第12回昆虫病理研究会シンポジウム  2016.9.1 

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  69. RNA-seq法を用いた核多角体病ウイルス感染カイコ細胞のトランスクリプトーム解析

    浜島りな, 佐藤昌直, 小林迪弘, 池田素子

    日本蚕糸学会第86回大会  2016.3.1 

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  70. AcMNPV HCF-1によるHycuMNPVのTn368細胞における増殖促進

    橘亜美, 浜島りな, 富崎萌, 近藤拓也, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第71回・東海支部第67回大会  2015.12.1 

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  71. Apsupとマイマイガイニシエーターカスパーゼとの相互作用解析

    斎藤綾, 浜島りな, 山田早人, 北口晃司, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第71回・東海支部第67回大会  2015.12.1 

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  72. カイコとマイマイガのIbm1によるアポトーシス誘導機構の解析

    富崎萌, 浜島りな, 岩本麻子, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第71回・東海支部第67回大会  2015.12.1 

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  73. マイマイガ核多角体病ウイルスを基にしたバクミドの作製

    窪田亮介, 浜島りな, 富崎萌, 橘亜美, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第71回・東海支部第67回大会  2015.12.1 

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  74. 核多角体病ウイルス感染に伴うRNA 分解誘導のチョウ目昆虫における保存性

    浜島りな, 青木智佐, 岩永将司, 小林淳, 李在萬, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第71回・東海支部第67回大会  2015.12.1 

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  75. AcMNPV hcf-1遺伝子を持つHycuMNPVバクミドの作出と感染性の調査

    橘亜美, 浜島りな, 富崎萌, 近藤拓也, 小林迪弘, 池田素子

    日本蚕糸学会第85回大会  2015.9.1 

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    Event date: 2015.9

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  76. Autographa californica核多角体病ウイルスP143のRNA分解誘導に関わるアミノ酸の決定

    浜島りな, 永峰俊弘, 小林迪弘, 池田素子

    日本蚕糸学会第85回大会  2015.9.1 

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  77. AcMNPV感染カイコ細胞が誘導するRNA分解に関わるAcMNPV P143の機能解析

    浜島りな, 小林迪弘, 池田素子

    第75回昆虫病理研究会  2015.9.1 

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  78. カイコとマイマイガにおけるp53, sir2, reaper相同体の機能解析

    富崎萌, 浜島りな, 岩本麻子, 小林迪弘, 池田素子

    日本蚕糸学会第85回大会  2015.9.1 

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  79. Multiple amino acid residues of Autographa californica MNPV P143 are responsible for ribosomal RNA degradation in Bombyx mori cells. International conference

    Hamajima, R, Kobayashi, M, Ikeda, M

    2015.8.1 

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    Language:English   Presentation type:Poster presentation  

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  80. Identification of the region of Autographa californica MNPV P143 responsible for ribosomal RNA degradation in Bombyx mori cells International conference

    Hamajima, R., Kobayashi, M., Ikeda, M.

    2015.4.1 

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    Event date: 2015.4

    Language:English   Presentation type:Oral presentation (general)  

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  81. Autographa californica核多角体病ウイルス感染によるカイコ細胞のRNA分解誘導

    浜島りな, 小林迪弘, 池田素子

    第59回日本応用動物昆虫学会大会  2015.3.1 

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  82. HycuMNPVバクミドの作製とバクミド由来ウイルスの感染性の調査

    橘亜美, 浜島りな, 富崎萌, 近藤拓也, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第70回・東海支部第66回大会  2014.10.1 

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    Event date: 2014.10

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  83. カイコ細胞が誘導するRNA分解におけるAcMNPV-P143ScH領域の機能解析

    浜島りな, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第70回・東海支部第66回大会  2014.10.1 

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  84. カイコとマイマイガにおけるp53, sir2, RHG遺伝子の同定と機能解析

    富崎萌, 浜島りな, 岩本麻子, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第70回・東海支部第66回大会  2014.10.1 

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  85. アメリカシロヒトリ核多角体病ウイルスのバクミド作成

    橘亜美, 浜島りな, 富崎萌, 近藤拓也, 小林迪弘, 池田素子

    第11回昆虫病理研究会シンポジウム  2014.9.1 

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    Event date: 2014.9

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  86. カイコ細胞のRNA分解誘導におけるAcMNPV-P143ScH領域の機能解析

    浜島りな, 小林迪弘, 池田素子

    第11回昆虫病理研究会シンポジウム  2014.9.1 

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    Event date: 2014.9

    Presentation type:Poster presentation  

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  87. カイコとマイマイガのアポトーシス誘導遺伝子の同定と機能解析

    富崎萌, 浜島りな, 岩本麻子, 小林迪弘, 池田素子

    第11回昆虫病理研究会シンポジウム  2014.9.1 

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    Event date: 2014.9

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  88. Autographa californica核多角体病ウイルスP143のRNA分解誘導に関わる領域の探索

    浜島りな, 永峰俊弘, 川崎祐, 松本正吾, 長田裕之, 小林迪弘, 池田素子

    日本蚕糸学会第84回大会  2014.4.1 

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    Event date: 2014.4

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  89. カイコ核多角体病ウイルス(BmNPV)とAcMNPVの分岐(ウイルス種分化)に関する進化的考察

    永峰俊弘, 浜島りな, 川崎祐, 松本正吾, 長田裕之, 今西重雄, 岩永将司, 小林迪弘, 池田素子

    日本蚕糸学会第84回大会  2014.4.1 

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    Event date: 2014.4

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  90. 核多角体病ウイルス感染カイコ細胞におけるBmp53, BmSirt2の機能解析

    富崎萌, 浜島りな, 岩本麻子, 小林迪弘, 池田素子

    日本蚕糸学会第84回大会  2014.4.1 

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    Event date: 2014.4

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  91. カイコアポトーシス関連遺伝子p53, sir2, ibm1のクローニングと機能解析

    富崎萌, 浜島りな, 岩本麻子, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第69回・東海支部第65回大会  2013.11.1 

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    Event date: 2013.11

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  92. カイコ細胞のrRNA分解誘導と宿主特異性決定に関与する核多角体病ウイルスP143の機能解析

    浜島りな, 永峰俊弘, 川崎祐, 松本正吾, 長田裕之, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第69回・東海支部第65回大会  2013.11.1 

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    Event date: 2013.11

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  93. BM-N細胞におけるrRNA分解誘導と宿主特異性決定に関わる核多角体病ウイルスP143の機能解析

    浜島りな, 永峰俊弘, 川崎祐, 松本正吾, 長田裕之, 伊藤勇弥, 小林迪弘, 池田素子

    第74回昆虫病理研究会  2013.9.1 

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    Event date: 2013.9

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  94. DNAへリカーゼP143の解析に基づくカイコ核多角体病ウイルス誕生に関する進化的考察

    永峰俊弘, 浜島りな, 川崎祐, 松本正吾, 長田裕之, 小林迪弘, 池田素子

    第74回昆虫病理研究会  2013.9.1 

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    Event date: 2013.9

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  95. AcMNPV bacmidを用いたBM-N細胞におけるrRNA分解機構解析

    浜島りな, 伊藤勇弥, 小林迪弘, 池田素子

    日本蚕糸学会第83回大会  2013.3.1 

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    Event date: 2013.3

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  96. AcMNPV感染に伴うBM-N細胞のRNA分解機構解析

    浜島りな, 伊藤勇弥, 小林迪弘, 池田素子

    日本蚕糸学会第66回東北支部・第68回中部支部・第64回東海支部・第78回関西支部・第68回九州支部合同大会  2012.11.1 

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    Event date: 2012.11

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  97. BM-N細胞IAP1におけるBm-Droncの制御

    岩本麻子, 浜島りな, 小林迪弘, 池田素子

    日本蚕糸学会第66回東北支部・第68回中部支部・第64回東海支部・第78回関西支部・第68回九州支部合同大会  2012.11.1 

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    Event date: 2012.11

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  98. AcMNPV感染BM-N細胞におけるリボソームRNA分解の機構解析

    浜島りな, 伊藤勇弥, 小林迪弘, 池田素子

    第10回昆虫病理研究会シンポジウム  2012.9.1 

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    Event date: 2012.9

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  99. Ribosomal RNA degradation during abortive nucleopolyhedrovirus infection of BM-N cells derived from the silkworm, Bombyx mori International conference

    Hamajima, R, Ito, Y, Kobayashi, M, Ikeda, M

    2012.8.1 

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    Event date: 2012.8

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  100. BM-N細胞におけるBmNPV-P143のNPV感染増殖への影響

    浜島りな, 伊藤勇弥, 光武宏, 小林淳, 小林迪弘, 池田素子

    日本蚕糸学会第82回大会  2012.3.1 

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    Event date: 2012.3

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  101. 種々のNPVp143遺伝子によるBM-N細胞のRNA分解誘導

    浜島りな, 伊藤勇弥, 光武宏, 小林淳, 小林迪弘, 池田素子

    日本蚕糸学会中部支部第67回・東海支部第63回研究発表会  2011.11.1 

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  102. Ribosomal RNA degradation as an antiviral response in silkworm cells Invited

    2021.7.31 

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Research Project for Joint Research, Competitive Funding, etc. 1

  1. 昆虫におけるリボソームの急速な分解を開始する分子機構の解明

    2024.4 - 2025.3

    2024年度徳島大学先端酵素学研究所「酵素学研究拠点」 共同利用  昆虫におけるリボソームの急速な分解を開始する分子機構の解明

    吉川 治孝

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    Authorship:Principal investigator 

    Grant amount:\150000

KAKENHI (Grants-in-Aid for Scientific Research) 5

  1. Molecular mechanisms of ribosome degradation in host defense of insect cells

    Grant number:22H02358  2022.4 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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  2. バキュロウイルスによる昆虫細胞のタンパク質合成能制御機構の解明と発現系への応用

    Grant number:21K14862  2021.4 - 2024.3

    日本学術振興会  科学研究費助成事業  若手研究

    浜島 りな

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    Authorship:Principal investigator 

    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

    バキュロウイルス発現系は、昆虫を宿主とするバキュロウイルスと昆虫細胞を組み合わせたタンパク質大量発現系であり、様々な分野で利用されている。その一方で、大量発現が困難な事例もあり、生産性の向上が大きな課題となっている。この発現系は、バキュロウイルスによる宿主細胞のタンパク質合成能の制御を利用したものであるが、それを担う分子機構は解明されていない。本研究では、感染細胞内におけるタンパク質合成変動の包括的な理解により、「バキュロウイルスがどのように細胞のタンパク質合成能を制御しているのか」を明らかにする。そして、得られた知見を基に、生産性を飛躍的に向上させた改良型タンパク質発現系の開発を目指す。
    バキュロウイルスは、宿主細胞の機能を高度に制御し、自身の増殖を遂行する。その最たるものは、細胞タンパク質の合成の遮断と、単一のウイルスタンパク質 (ポリへドリン) の爆発的な発現誘導であり、感染の最終段階では、ポリへドリンは結晶体を形成し、その量は細胞の全タンパク質量の約30-50%という驚くべき割合になる。しかし、大量発現を担う分子機構の詳細は解明されていない。本研究は、バキュロウイルスが単一のウイルスタンパク質の大量発現をどのように達成するのかを明らかにすることを目的として行う。
    本年度は、ポリヘドリンプロモーター制御下の遺伝子の大量発現を担う分子機構について、より容易に解析を進めることを目的として、ポリヘドリンプロモーターとレポーター遺伝子を保有するプラスミドを作出し、バキュロウイルス感染によりレポーター遺伝子の大量発現が誘導されるかどうかを調査した。その結果、今回作出したプラスミドではレポーター遺伝子の大量発現が誘導されない可能性が示され、ポリヘドリンプロモーターがウイルスゲノム上に位置することが大量発現を達成する上で重要であることが考えられた。今後、さらなる検証を進めていく予定である。また、リボソームに着目した解析を行うため、ショ糖密度勾配遠心法による昆虫細胞のリボソーム精製法の検討を進めた。40S、60Sリボソームの分画については引き続き検討を続ける必要があるが、80Sリボソームについては高い再現性での分画が可能となった。
    さらに、我々の研究室で進めている昆虫細胞のバキュロウイルス感染に対する応答の解析から、バキュロウイルス感染を負に制御する機能をもつと予測される宿主タンパク質を見出し、この宿主タンパク質の発現を抑制すると、ウイルスタンパク質の発現量が有意に減少することを明らかにした。この成果をまとめた論文については、国際誌にて査読を受け、現在再投稿準備中である。
    ポリヘドリンプロモーター制御下の遺伝子の大量発現を担う分子機構を解明するために、昨年度作出したポリへドリンプロモーターによりレポーター遺伝子を発現するレポーターウイルスに加え、ポリヘドリンプロモーターとレポーター遺伝子を保有するプラスミドをベースの実験系、ショ糖密度勾配遠心法によるリボソーム精製法の確立を進めている。また、昆虫細胞のバキュロウイルス感染に対する応答の解析から、バキュロウイルス感染を負に制御する機能をもつと予測される宿主タンパク質を見出し、当初の研究計画とは異なる方向性からも、バキュロウイルスが単一のウイルスタンパク質の大量発現を達成する分子機構の解明に向けたアプローチを進めている。これらのことから、当初の研究計画よりも予備実験が必要であるものの、研究開始当初には想定していなかった宿主タンパク質の発見もあったため、研究目的の達成に向けて、おおむね順調に進展していると評価した。
    引き続き、ショ糖密度勾配遠心法によるリボソームの精製法と、昨年度取り組んだタグ抗体を用いた免疫沈降によるリボソームの精製法について、条件の検討を進めて高い再現性で分画できる手法を確立し、バキュロウイルス感染時の昆虫細胞のリボソームの挙動について調査を行う。また、ポリヘドリンプロモーターがウイルスゲノム上に位置することが大量発現を達成する上で重要である可能性について、さらなる検証を行い、ポリヘドリンプロモーター制御下の遺伝子の大量発現を担う分子機構の解明に寄与する知見を得たい。さらに、昆虫細胞のバキュロウイルス感染に対する応答の解析から見出したバキュロウイルス感染を負に制御する機能をもつと予測される宿主タンパク質について、どのような分子機構でバキュロウイルスのタンパク質産生を抑制しているのかを明らかにし、バキュロウイルスがどのように自身のタンパク質合成を促進しているのかを解明する糸口としたい。

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  3. Elucudation of the significance of hidden break structure in insect ribosome

    Grant number:18K14474  2018.4 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    HAMAJIMA Rina

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\3250000 ( Direct Cost: \2500000 、 Indirect Cost:\750000 )

    The ribosome structure is highly conserved from yeast to humans, while the insect ribosome generally has a characteristic structure, called hidden break. To elucidate the significance of hidden break, I tried to construct the experimental model using yeast. I found that the growing of yeast was significantly reduced by introducing the rRNA sequence with hidden break site and the ribosomal protein located near the hidden break site, both of which derived from insect, indicating that introduced rRNA sequence and ribosomal protein have a negative influence on ribosome synthesis or stability in yeast. These results suggest that insect-specific factor(s) or mechanism(s) may be needed for the synthesis of ribosome with hidden break.

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  4. 真核生物におけるリボソームの分解経路とその制御機構の解明

    Grant number:17J00694  2017.4 - 2020.3

    日本学術振興会  科学研究費補助金  特別研究員奨励費

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

  5. 核多角体病ウイルス感染昆虫細胞におけるRNA分解による生体防御の分子機構

    Grant number:15J02649   2015.4 - 2017.3

    日本学術振興会  科学研究費補助金  特別研究員奨励費

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\2170000 ( Direct Cost: \1900000 、 Indirect Cost:\270000 )

 

Teaching Experience (On-campus) 22

  1. Insect Pathology

    2023

  2. Insect Science 2

    2023

  3. Insect Science 1

    2023

  4. 資源生物科学基盤実験実習

    2023

  5. 資源生物科学実験実習

    2023

  6. 生物学実験

    2023

  7. 農学セミナー1

    2023

  8. Insect Pathology

    2022

  9. Insect Science 2

    2022

  10. Insect Science 1

    2022

  11. 資源生物科学実験実習

    2022

  12. 生物学実験

    2022

  13. 農学セミナー1

    2022

  14. 資源生物科学基盤実験実習

    2022

  15. Insect Pathology

    2021

  16. 資源生物科学基盤実験実習

    2021

  17. 資源生物科学実験実習

    2021

  18. 生物学実験

    2021

  19. Insect Science 1

    2021

  20. Insect Science 2

    2021

  21. 農学セミナー1

    2021

  22. 資源生物科学実験実習2

    2020

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Teaching Experience (Off-campus) 1

  1. KITライフサイエンスセミナー

    2022.11 Kyoto Institute of Technology)