担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：American Journal of Clinical Pathology  

Objectives: Cytomorphology is known to differ depending on the processing technique, and these differences pose a problem for automated diagnosis using deep learning. We examined the as-yet unclarified relationship between cell detection or classification using artificial intelligence (AI) and the AutoSmear (Sakura Finetek Japan) and liquid-based cytology (LBC) processing techniques. Methods: The “You Only Look Once” (YOLO), version 5x, algorithm was trained on the AutoSmear and LBC preparations of 4 cell lines: lung cancer (LC), cervical cancer (CC), malignant pleural mesothelioma (MM), and esophageal cancer (EC). Detection and classification rates were used to evaluate the accuracy of cell detection. Results: When preparations of the same processing technique were used for training and detection in the 1-cell (1C) model, the AutoSmear model had a higher detection rate than the LBC model. When different processing techniques were used for training and detection, detection rates of LC and CC were significantly lower in the 4-cell (4C) model than in the 1C model, and those of MM and EC were approximately 10% lower in the 4C model. Conclusions: In AI-based cell detection and classification, attention should be paid to cells whose morphologies change significantly depending on the processing technique, further suggesting the creation of a training model.

DOI： 10.1093/ajcp/aqac178

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担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1159/000526131

担当区分：最終著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Acta Cytologica  

Introduction: Liquid-based cytology (LBC)-fixed samples can be used for preparing multiple specimens of the same quality and for immunocytochemistry (ICC); however, LBC fixing solutions affect immunoreactivity. Therefore, in this study, we examined the effect of LBC fixing solutions on immunoreactivity. Methods: Samples were cell lines, and specimens were prepared from cell blocks of 10% neutral buffered formalin (NBF)-fixed samples and the four types of LBC-fixed samples: PreservCyt®, CytoRich™ Red, CytoRich™ Blue, and TACAS™ Ruby, which were post-fixed with NBF. ICC was performed using 24 different antibodies, and immunocytochemically stained specimens were analyzed for the percentage of positive cells. Results: Immunoreactivity differed according to the type of antigen detected. For nuclear antigens, the highest percentage of positive cells of Ki-67, WT-1, ER, and p63 was observed in the NBF-fixed samples, and the highest percentage of positive cells of p53, TTF-1, and PgR was observed in the TACAS™ Ruby samples. For cytoplasmic antigens, the percentage of positive cells of CK5/6, Vimentin, and IMP3 in LBC-fixed samples was higher than or similar to that in NBF-fixed samples. The percentage of positive cells of CEA was significantly lower in CytoRich™ Red and CytoRich™ Blue samples than in the NBF-fixed sample (p < 0.01). Among the cell membrane antigens, the percentage of positive cells of Ber-EP4, CD10, and D2-40 was the highest in NBF-fixed samples and significantly lower in CytoRich™ Red and CytoRich™ Blue samples than that in NBF-fixed samples (p < 0.01). The NBF-fixed and LBC-fixed samples showed no significant differences in the percentage of positive cells of CA125 and EMA. Discussion/Conclusion: ICC using LBC-fixed samples showed the same immunoreactivity as NBF-fixed samples when performed on cell block specimens post-fixed with NBF. The percentage of positive cells increased or decreased based on the type of fixing solution depending on the amount of antigen in the cells. Further, the detection rate of ICC with LBC-fixed samples varied according to the type of antibody and the amount of antigen in the cells. Therefore, we propose that ICC using LBC-fixed samples, including detection methods, should be carefully performed.

DOI： 10.1159/000526131

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： https://doi.org/10.1159/000526098

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Journal of Biological Chemistry  

Bone marrow development and endochondral bone formation occur simultaneously. During endochondral ossification, periosteal vasculatures and stromal progenitors invade the primary avascular cartilaginous anlage, which induces primitive marrow development. We previously determined that bone marrow podoplanin (PDPN)-expressing stromal cells exist in the perivascular microenvironment and promote megakaryopoiesis and erythropoiesis. In this study, we aimed to examine the involvement of PDPN-expressing stromal cells in postnatal bone marrow generation. Using histological analysis, we observed that periosteum-derived PDPN-expressing stromal cells infiltrated the cartilaginous anlage of the postnatal epiphysis and populated on the primitive vasculature of secondary ossification center. Furthermore, immunophenotyping and cellular characteristic analyses indicated that the PDPN-expressing stromal cells constituted a subpopulation of the skeletal stem cell lineage. In vitro xenovascular model cocultured with human umbilical vein endothelial cells and PDPN-expressing skeletal stem cell progenies showed that PDPN-expressing stromal cells maintained vascular integrity via the release of angiogenic factors and vascular basement membrane-related extracellular matrices. We show that in this process, Notch signal activation committed the PDPN-expressing stromal cells into a dominant state with basement membrane-related extracellular matrices, especially type IV collagens. Our findings suggest that the PDPN-expressing stromal cells regulate the integrity of the primitive vasculatures in the epiphyseal nascent marrow. To the best of our knowledge, this is the first study to comprehensively examine how PDPN-expressing stromal cells contribute to marrow development and homeostasis.

DOI： 10.1016/j.jbc.2022.101833

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Physiological Reports  

Phosphatidylinositol 3-kinase (PI3K) signaling promotes the differentiation and proliferation of regulatory B (Breg) cells, and the lipid phosphatase phosphatase and tensin homolog deleted on chromosome 10 (PTEN) antagonizes the PI3K–Akt signaling pathway. We previously demonstrated that cardiac Akt activity is increased and that restraint stress exacerbates hypertension and both heart and adipose tissue (AT) inflammation in DS/obese rats, an animal model of metabolic syndrome (MetS). We here examined the effects of restraint stress and pharmacological inhibition of PTEN on heart and AT pathology in such rats. Nine-week-old animals were treated with the PTEN inhibitor bisperoxovanadium-pic [bpV(pic)] or vehicle in the absence or presence of restraint stress for 4 weeks. BpV(pic) treatment had no effect on body weight or fat mass but attenuated hypertension in DS/obese rats subjected to restraint stress. BpV(pic) ameliorated left ventricular (LV) inflammation, fibrosis, and diastolic dysfunction as well as AT inflammation in the stressed rats. Restraint stress reduced myocardial capillary density, and this effect was prevented by bpV(pic). In addition, bpV(pic) increased the proportions of Breg and B-1 cells as well as reduced those of CD8+ T and B-2 cells in AT of stressed rats. Our results indicate that inhibition of PTEN by bpV(pic) alleviated heart and AT inflammation in stressed rats with MetS. These positive effects of bpV(pic) are likely due, at least in part, to a reduction in blood pressure, an increase in myocardial capillary formation, and an altered distribution of immune cells in fat tissue that result from the activation of PI3K–Akt signaling.

DOI： 10.14814/phy2.15165

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その他リンク： https://onlinelibrary.wiley.com/doi/full-xml/10.14814/phy2.15165

担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Acta Cytologica  

Introduction: Liquid-based cytology (LBC) is increasingly used for nongynecologic applications. However, the cytological preparation of LBC specimens is influenced by the processing technique and the preservative used. In this study, the influence of the processing techniques and preservatives on cell morphology was examined mathematically and statistically. Methods: Cytological specimens were prepared using the ThinPrep (TP), SurePath (SP), and AutoSmear methods, with 5 different preservative solutions. The cytoplasmic and nuclear areas of Papanicolaou-stained specimens were measured for all samples. Results: The cytoplasmic and nuclear areas were smaller in cells prepared using the 2 LBC methods, compared to that prepared using the AutoSmear method, irrespective of the preservative used. The cytoplasmic and nuclear areas of cells prepared using the SP method were smaller than those of cells prepared using the TP method, irrespective of the preservative used. There were fewer differences among the cytoplasmic areas of cells prepared with different preservative solutions using the TP method; however, the cytoplasmic areas of cells prepared using the SP method changed with the preservative solution used. Conclusions: The most significant difference affecting the cytoplasmic and nuclear areas was the processing technique. The TP method increased the cytoplasmic and nuclear areas, while the methanol-based PreservCyt solution enabled the highest enlargement of the cell. LBC is a superior preparation technique for standardization of the specimens. Our results offer a better understanding of methods suitable for specimen preparation for developing precision AI-based diagnosis in cytology.

DOI： 10.1159/000519335

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Acta Cytologica  

Introduction: Deep learning is a subset of machine learning that has contributed to significant changes in feature extraction and image classification and is being actively researched and developed in the field of cytopathology. Liquid-based cytology (LBC) enables standardized cytological preparation and is also applied to artificial intelligence (AI) research, but cytological features differ depending on the LBC preservative solution types. In this study, the relationship between cell detection by AI and the type of preservative solution used was examined. Methods: The specimens were prepared from five preservative solutions of LBC and stained using the Papanicolaou method. The YOLOv5 deep convolutional neural network algorithm was used to create a deep learning model for each specimen, and a BRCPT model from five specimens was also created. Each model was compared to the specimen types used for detection. Results: Among the six models, a difference in the detection rate of approximately 25% was observed depending on the detected specimen, and within specimens, a difference in the detection rate of approximately 20% was observed depending on the model. The BRCPT model had little variation in the detection rate depending on the type of the detected specimen. Conclusions: The same cells were treated with different preservative solutions, the cytologic features were different, and AI clarified the difference in cytologic features depending on the type of solution. The type of preservative solution used for training and detection had an extreme influence on cell detection using AI. Although the accuracy of the deep learning model is important, it is necessary to understand that cell morphology differs depending on the type of preservative solution, which is a factor affecting the detection rate of AI.

DOI： 10.1159/000526098

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1152/ajpheart.00657.2020

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/nyas.14546

記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.3892/ol.2020.12073

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Nutrition and Diabetes  

Objectives: Evidence suggests that visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) should be considered as distinct types of white fat. Although VAT plays a key role in metabolic syndrome (MetS), the role of subcutaneous adipose tissue (SAT) has been unclear. DahlS.Z-Leprfa/Leprfa (DS/obese) rats, an animal model of MetS, develop adipocyte hypertrophy and inflammation to similar extents in SAT and VAT. We have now investigated the effects of salt loading and SAT removal on cardiac, renal, and VAT pathology in DS/obese rats. Methods: DS/obese rats were subjected to surgical removal of inguinal SAT or sham surgery at 8 weeks of age. They were provided with a 0.3% NaCl solution as drinking water or water alone for 4 weeks from 9 weeks of age. Results: Salt loading exacerbated hypertension, insulin resistance, as well as left ventricular (LV) hypertrophy, inflammation, fibrosis, and diastolic dysfunction in DS/obese rats. It also reduced both SAT and VAT mass but aggravated inflammation only in VAT. Although SAT removal did not affect LV hypertrophy in salt-loaded DS/obese rats, it attenuated hypertension, insulin resistance, and LV injury as well as restored fat mass and alleviated inflammation and the downregulation of adiponectin gene expression in VAT. In addition, whereas salt loading worsened renal injury as well as upregulated the expression of renin–angiotensin-aldosterone system-related genes in the kidney, these effects were suppressed by removal of SAT. Conclusions: SAT removal attenuated salt-induced exacerbation of MetS and LV and renal pathology in DS/obese rats. These beneficial effects of SAT removal are likely attributable, at least in part, to inhibition of both VAT and systemic inflammation.

DOI： 10.1038/s41387-020-00132-1

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1159/000503268

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1159/000500769

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： http://doi.org/10.1038/s41387-020-00132-1

担当区分：最終著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：The Society of Analytical Bio-Science [編]  

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Journal of Cytology  

Background: Small round cell tumors (SRCTs) are a group of tumors composed of small, round, and uniform cells with high nuclear/cytoplasmic (N/C) ratios. The appearance of SRCT neoplastic cells in the effusion fl uid is very rare. We reported the cytomorphological fi ndings of SRCTs in effusion cytology, and performed statistical and mathematical analyses for a purpose to distinguish SRCTs. Materials and Methods: We analyzed the cytologic fi ndings of effusion samples from 40 SRCT cases and measured the lengths of the nuclei, cytoplasms, and the cell cluster areas. The SRCT cases included 14 Ewing sarcoma (EWS)/primitive neuroectodermal tumor cases, 5 synovial sarcoma cases, 6 rhabdomyosarcoma cases, 9 small cell lung carcinoma (SCLC) cases, and 6 diffuse large B-cell lymphoma (DLBL) cases. Results: Morphologically, there were no signifi cant differences in the nuclear and cytoplasmic lengths in cases of EWS, synovial sarcoma, and rhabdomyosarcoma. The cytoplasmic lengths in cases of SCLC and DLBL were smaller than those of EWS, synovial sarcoma, and rhabdomyosarcoma. The nuclear density of the cluster in SCLC was higher than that in other SRCTs, and cases of DLBL showed a lack of anisokaryosis and anisocytosis. Conclusion: We believe that it might be possible to diagnose DLBL and SCLC from cytologic analysis of effusion samples but it is very diffi cult to use this method to distinguish EWS, synovial sarcoma, and rhabdomyosarcoma. Statistical andmathematical analyses indicated that nuclear density and dispersion of nuclear and cytoplasmic sizes are useful adjuncts to conventional cytologic diagnostic criteria, which are acquired from experience.

DOI： 10.4103/0970-9371.182529

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/his.12356

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Oncology Reports  

The Working Group of the Japanese Society of Clinical Cytology was assembled to assess the current status of breast cytology in Japan by conducting a large-scale survey regarding the accuracy of fine-needle aspiration biopsy (FNAB) in Japan. We collected data and investigated the status of breast cytological diagnosis at 12 different cooperating facilities in Japan, and re-evaluated their false-negative and false-positive cases. Among 30,535 individuals who underwent a breast cytological examination, analyses were conducted on 10,890 individuals (35.7%) in whom cytological diagnoses were confirmed by histology. Among these patients, the cytological diagnosis had an inadequate rate of 17.7%, an indeterminate rate of 7.8%, a positive predictive value of 'malignancy suspected' cells of 92.4%, an absolute sensitivity of 76.7%, a complete sensitivity of 96.7%, a specificity of 84.3%, a positive predictive value of 'malignant' cells of 99.5%, a false-negative value of 3.31%, a false-positive value of 0.25% and an accuracy rate of 88.0%. Subsequently, 297 false-negative and 23 false-positive cases were re-evaluated and several factors were characterized (i.e. histological type, tumor size and misread points). This survey collected data from a large number of cases for breast FNAB. Based on our survey, the accuracy of FNAB in Japan was relatively high compared with the goal of assessment of diagnostic accuracy. However, there were some false-negative and false-positive cases. Improvements in accuracy resulting from the learning points in the present study will lead to more useful and reliable diagnostic tools in clinical practice.

DOI： 10.3892/or.2012.2014

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：American Journal of Clinical Pathology  

The most commonly used fixative in effusion cytology is formalin. In the present study, the immunocytochemical properties of formalin-fixed and alcohol-fixed specimens were compared to evaluate the usefulness of alcohol-fixed cytocentrifuged preparations for routine cytologic diagnosis. A total of 269 effusion samples and 17 primary antibodies were used. The sensitivity of immunocytochemical studies in alcoholfixed specimens was similar and correlated to that of formalin-fixed specimens, suggesting that alcohol-fixed cytocentrifuged preparations are useful in effusion cytology. Pretreatment with or without heat-induced antigen retrieval revealed that antigen retrieval was unnecessary for immunocytochemical studies with most primary antibodies in alcohol-fixed cytocentrifuged preparations. The present study describes the use of immunocytochemical studies with alcohol-fixed cytocentrifuged preparations for diagnosis in routine effusion cytology. © American Society for Clinical Pathology.

DOI： 10.1309/AJCPAH5WR2LFTUSI

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Diagnostic Cytopathology  

To differentiate reactive mesothelial cells (RMs) from metastatic carcinoma and malignant mesothelioma (MM) in effusion cytology is crucial for the cytologic diagnosis and the management of the patients. In the present study, the immunocytochemical staining profile of the epithelial membrane antigen (EMA), the insulin-like growth factor-II mRNA-binding protein 3 (IMP3), and the glucose transporter-1 (GLUT-1) was examined to distinguish RMs from malignant cells. A total of 171 pleural (n = 87) and peritoneal (n = 84) effusion specimens, including 50 benign effusions with RMs, 11 MM effusions, and 110 metastatic malignant effusions, were evaluated for immunocytochemistry. EMA, IMP3, monoclonal GLUT-1, and polyclonal GLUT-1 immunoreactivity were observed in 26.0%, 6.0%, 20.0%, and 18.0% of RMs, respectively. In contrast to RMs, the immunoreactivity in MM was 100%, 36.4%, 100%, and 90.9%; adenocarcinoma (AC) was 100%, 80.8%, 81.7%, and 72.1%; squamous-cell carcinoma was 83.3%, 83.3%, 83.3%, and 66.7%. EMA, IMP3, mGLUT-1, and pGLUT-1 expressions were observed in 98.4%, 65.6%, 88.5%, and 75.4% in the pleural effusion with malignant cells, and 100%, 88.3%, 78.3%, and 71.7% in ascites containing malignant cells, respectively. The findings of the present study indicate that the immunocytochemical staining for EMA, IMP3, and GLUT-1 is a useful diagnostic tool for distinguishing effusions containing malignant cells from those that contain benign cells, and in particular, we suggest that the combination of mGLUT-1 and EMA, and IMP3 and EMA are extremely useful in pleural effusion and in ascites, respectively. © 2010 Wiley-Liss, Inc.

DOI： 10.1002/dc.21398

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1016/j.acthis.2009.08.007

担当区分：筆頭著者,　責任著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：神奈川県臨床細胞学会  

背景:良性腫瘍としては比較的まれな腫瘍である基底細胞腺腫2例の細胞形態学的特徴とともに、細胞学的鑑別所見について文献的考察を加え報告する。症例:(1)68歳、女性。右耳下部に硬結を自覚し、当院耳鼻咽喉科を受診。穿刺吸引細胞診が施行された。細胞標本では、血管間質を伴い、重積性を有する結合性の強い集塊を認めた。個々の腫瘍細胞は小型、類円形〜楕円形核を有し、大小不同および核異型に乏しかった。(2)36歳、女性。左耳下部に10年以上前より腫瘤を感じていたが放置、手術目的のため当院耳鼻咽喉科を紹介受診され、穿刺吸引細胞診が施行された。細胞標本では、結合性が強く、境界明瞭で、palisadingをうかがわせる細胞集塊を認め、集塊辺縁にはライトグリーン好性の基底膜様物質を伴っていた。個々の腫瘍細胞は小型で大小不同に乏しく、メイギムザ染色では集塊辺縁および内部に異染性が認められた。結論:基底細胞腺腫は増殖形態から組織、細胞像ともに多様である。細胞診断に際しては、小型・裸核状で、多形性・異型性に乏しい基底細胞様細胞の、結合性の強い細胞集塊と、メイギムザ染色を併用した基底膜様物質の確認は診断の一助になると考えられた。(著者抄録)

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Human Pathology  

Differentiating reactive mesothelial cells from metastatic carcinoma and malignant mesothelioma is critical in effusion cytology. Numerous immunohistochemical/cytochemical reports use various antibodies in effusion samples, and most antibodies differentiate metastatic adenocarcinoma from malignant mesothelioma, but no antibodies help distinguish malignant mesothelioma from reactive mesothelial cells. A mouse monoclonal antibody (IMP3/L523S) against KOC is a 580-amino acid oncofetal RNA-binding protein containing 4 K homology domains. IMP3/L523S has been identified in several human malignant tumors. The immunocytochemical staining profile of IMP3 was determined in 95% alcohol-fixed cytologic effusion specimens. A total of 229 cases of pleural and peritoneal effusion cytospecimens were evaluated for the study, including 39 benign effusions with reactive mesothelial cells and 190 metastatic malignant effusions. IMP3 immunoreactivity was observed in 2 (5.1%) of 39 cases of reactive mesothelial cells, 138 (72.6%) of 190 cases of malignant effusion, 4 (36.4%) of 11 cases of malignant mesothelioma, 106 (75.7%) of 140 cases of metastatic adenocarcinoma, and 8 (100%) of 8 cases of squamous cell carcinoma. The overall specificity for the diagnosis of malignancy was 94.9%, whereas the sensitivity was 72.6%. In the peritoneal effusions, the sensitivity for the diagnosis of metastatic adenocarcinoma to distinguish reactive mesothelial cells was 92.3%. In conclusion, IMP3 staining is present in many carcinomas and is not a useful marker for distinguishing between carcinomas arising in different organs. However, the IMP3 antibody is a highly specific marker for malignant lesions, and thus, IMP3 staining is useful for distinguishing neoplastic cells from reactive mesothelial cells in effusion samples. © 2010 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.humpath.2009.04.030

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Diagnostic Cytopathology  

Effusion cytological analyses of amelanotic malignant melanoma (AMM) are very rare and no concise description of AMM related cytomorphologic features using effusion have been reported. Here, we report the cytomorphological, immunohistochemical, and immunocytochemical findings in the effusion cytology of six cases of malignant melanoma (MM), one case of AMM, and five cases of melanotic malignant melanoma. Papanicolaou-stained smears exhibited conspicuous nucleoli, multinucleation, and cytoplasmic vacuolization in all of the MM cases. In addition, the AMM case displayed numerous mitotic figures and intranuclear cytoplasmic inclusions. With regard to the immunohistochemistry findings, all six cases of melanoma were positive for Melan-A/MART-1, HBME-1, and S-100. In the immunohistochemistry analyses, five of six cases of melanoma were positive for WT-1, as was the AMM specimen. Furthermore, because the effusion analysis of malignant mesothelioma proved positive for WT-1, it should be noted that WT-1 effusion analysis is not an appropriate means to distinguish between MM and malignant mesothelioma. We suggest that it is important to recognize cytomorphologic characteristics, such as melanin pigment, conspicuous nucleoli, multinucleation, and cytoplasmic vacuolization, and to choose appropriate antibodies for the correct diagnosis of MM in effusion. © 2009 Wiley-Liss, Inc.

DOI： 10.1002/dc.21054

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Diagnostic Cytopathology  

Well-differentiated papillary mesothelioma (WDPM), a distinct subtype of diffuse malignant mesothelioma, usually occurs in the peritoneum and is seen most commonly in women of reproductive age. Histologic features of WDPM include papillary growth and stout fibrous cores surrounded by a single layer of tumor cells. We present the case of a 73-year-old woman without subjective symptoms who showed signs of peritoneal effusion during a routine examination and for whom cytologic examination of the ascitic fluid was performed. Many spherical clusters, with a smooth external surface composed of a single layer of uniform cuboidal cells, were observed. Within each cluster, a collagenous ball showed light green Papanicolaou staining. Immunohistochemistry of surgical specimens showed tumor cells positive for calretinin, D2-40, and HBME-1 staining. The histologic diagnosis was WDPM. The identification of a collagenous ball within these clusters is a useful cytologic finding for the diagnosis of WDPM. WDPM should be suspected when numerous collagenous balls are present by effusion cytology and isolated cells are not. © 2008 Wiley-Liss, Inc.

DOI： 10.1002/dc.20824

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Gynecologic Oncology  

Objective: Several Cytokeratin (CK) isoforms have been analyzed in cervical intraepithelial lesions. However, previously reported numbers of specimens have been too low to evaluate any correlation between CK and CIN. Methods: We examined the immunohistochemical staining of p16, CK8, and CK17 in 134 cervical tissues obtained by punch biopsy and graded as follows: CIN I (n = 39), CIN II (n = 31), CIN III (n = 43), SCC (n = 21). Results: p16 staining was identified in 74.4% of CIN I, 93.6% of CIN II, 97.7% of CIN III, and 100% of SCC cases. CK8 and CK17 staining were identified in 12.8% and 33.3% of CIN I, 22.6% and 58.1% of CIN II, 62.8% and 81.4% of CIN III, and 71.4% and 95.2% of SCC cases, respectively. Interestingly, positivity for CK8 and CK17 correlated with increasing lesion grade of the intraepithelial lesions and also correlated with p16 staining (p16, p = 0.0008; CK8, p < 0.0001, and CK17, p < 0.0001), and a coordinate expression profile of CK8[+]/CK17[+] correlates with increasing CIN grade and carcinoma (likewise, a coordinate expression profile of CK8[-]/CK17[-] correlates with decreasing CIN grade and absence of carcinoma), but expression of just CK8 (CK8[+]/CK17[-]) or just CK17 (CK8[-]/CK17[+]) does not correlate with increasing CIN grade and carcinoma. Conclusions: Results of the present study showed that p16, CK8, and CK17 immunostaining differed according to the degree of cervical intraepithelial lesions and SCC, and surprisingly, that staining was significantly correlated with increasing lesion grade of CIN and SCC.

DOI： 10.1016/j.ygyno.2007.11.042

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記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Pathology Research and Practice  

We report a case of a clear cell adenocarcinoma arising from a giant cystic adenomyosis, with immunohistochemical analysis of p53 and laminin-5 gamma2 chain overexpression. Microscopically, not only clear cell adenocarcinoma showing myometrial invasion but also single-layered clear cell adenocarcinoma cells lining the cyst wall were observed. Transition from these single-layered tumor cells to papillary proliferative lesions of various degrees was recognized. Moreover, these tumor cells were continuous with minimal atypical cells. Although the tumor cells within the uterus showed a low positive cell ratio for p53, the metastatic foci showed a remarkable p53 overexpression. Laminin-5 gamma2 chain expression was low in papillary proliferation and high in myometrial invasion and metastatic foci. The single-layered tumor cells showing non-invasive proliferation also contained laminin-5 gamma2 chain-positive cells. When non-invasive tumor cells were considered to be at an early stage in tumor progression, some tumor cells had already acquired an invasive feature. p53 overexpression was not related to expression of the laminin-5 gamma2 chain. © 2008 Elsevier GmbH. All rights reserved.

DOI： 10.1016/j.prp.2008.02.005

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/j.1365-2559.2007.02742.x

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Diagnostic Cytopathology  

We describe the fine-needle aspiration cytology features of a primary parathyroid cancer and of the local recurrent and distant metastatic lesions. The presence of prognostic factors Ki-67 and proliferating cell nuclear antigen (PCNA) was compared immunohistochemically between primary parathyroid carcinoma and related metastatic and recurrent foci. Flow cytometric DNA analysis was also performed to investigate any chromosomal abnormality of the parathyroid carcinoma. Cytologic examination of the endocrine tumor showed that it comprised a loose cohesive cluster and tumor cells with granular cytoplasm and mild nuclear atypia, but for purposes of cytodiagnosis, it is difficult to determine whether such a neoplasm is malignant on the basis of morphology alone. Immunohistochemical analysis showed that Ki-67 and PCNA labeling indices were higher in the recurrent and metastasized carcinomas than in the primary cancer, suggesting that neoplastic cells become more malignant in the recurrent and metastasized foci. To our knowledge, this is the first report describing not only cytopathologic but also immunocytologic differences between primary parathyroid cancer and the metastatic lesion. © 2005 Wiley-Liss, Inc.

DOI： 10.1002/dc.20399

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.18926/AMO/31955

PubMed

記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Acta Medica Okayama  

We describe a novel method for immunofluorescent detection of multiple antigens in a single paraffin-embedded tissue section. We hypothesized that if fluorescent dyes are resistant to heat treatment, then thermal inactivation of immunoglobulins during antigen detection procedures might make it possible to use multicolor immunofluorescence detection even if the primary antibodies are from the same species. We found that several fluorescent dyes, including fluorescein isothiocyanate (FITC), Cy3 and Cy5, were resistant to heating at 90°C for 15 min, whereas the antigenicities of the primary antibodies were lost completely. This novel method, which uses heat treatment between staining steps, has great advantages for multicolor immunofluorescence because unlabeled primary antibodies from the same species can be used. Therefore, by using this method not only 3 unlabeled mouse monoclonal antibodies but also 3 unlabeled rabbit antisera can be used as primary antibodies for multicolor immunofluorescence. Copyright©2005 by Okayama University Medical School.

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Diagnostic Cytopathology  

We describe unusual cytologic features of the peritoneal fluid in a patient with immature ovarian teratoma. Immature embryonal and neuroectodermal components of such tumors are rarely observed in the ascites; to our knowledge, there have been only three reports of cytologic findings of immature teratoma cells in the ascites. Our patient was a 26-yr-old woman who presented with a huge pelvic mass. A grade 3 immature right ovarian teratoma was diagnosed pathologically. Cytologic examination of the ascitic fluid revealed a variety of neoplastic cells, including immature neuroepithelial cells forming rosette-like structures, keratinized squamous cells, squamoid metaplastic cells, and immature glial-appearing cells, Immunohistochemical analysis revealed that immature gastrointestinal elements and yolk sac elements were positive for α-fetoprotein. This is the first description of the cytologic features of non-neuroepithelial elements in the ascitic fluid in a patient with immature ovarian teratoma. © 2005 Wiley-Liss, Inc.

DOI： 10.1002/dc.20259

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担当区分：筆頭著者,　責任著者   記述言語：英語   掲載種別：研究論文（学術雑誌）   出版者・発行元：Gynecologic Oncology  

Objective. Expression of Matrix metalloproteinase 7 (MMP-7) and Ki-67 by carcinoma components (CCs) and sarcoma components (SCs) in carcinosarcoma of the female reproductive organs has been investigated by conventional methods, but analysis with immunohistochemical staining of multiple antigens has not been reported. Methods. We report the profiles of expression of MMP-7 and Ki-67 in carcinosarcoma determined with immunohistochemical staining techniques. We used antibodies against epithelial antigen (EA), epithelial membrane antigen (EMA), and vimentin for immunofluorescence double staining of ascitic fluid in eight cases of carcinosarcoma of female reproductive organs. We also used immunohistochemical triple staining to compare MMP-7 and Ki-67 expression between CCs and SCs in the primary site of carcinosarcoma. Results. Immunofluorescence analysis revealed that all neoplastic cells in the ascitic fluid were positive for EA or EMA, indicating that these cells were CCs. Immunohistochemical analyses of the primary organ of carcinosarcoma revealed that MMP-7 was expressed on CCs in four of eight cases of carcinosarcoma, whereas MMP-7 was not expressed on SCs. The average Ki-67 labeling index (LI) in CCs and SCs was 51.8% and 28.6%, respectively. The difference in Ki-67 LI between CCs and SCs was statistically significant (t test for paired samples, P = 0.0173). Conclusions. This is the first study to examine carcinosarcoma of the female reproductive organ by immunohistochemical staining for multiple antigens, which allows analysis of mixed tumor elements. In addition, we found that expression of MMP-7 and the average Ki-67 LI differ between CCs and SCs in carcinosarcoma. The predominance of CCs as the malignant cells in the ascitic fluid may be due to cytological differences between CCs and SCs of carcinosarcoma. © 2004 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.ygyno.2004.12.024

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担当区分：筆頭著者   記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1159/000326155

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記述言語：英語   掲載種別：研究論文（学術雑誌）  

DOI： 10.1111/j.1440-1827.2004.01671.x

PubMed

記述言語：日本語  

担当区分：筆頭著者,　責任著者   記述言語：日本語   掲載種別：研究論文（学術雑誌）  

記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：(一社)日本臨床衛生検査技師会  

微小抗原を用いて高感度に抗原検出する方法を比較検討した結果,アミノ酸ポリマーを用いているSimple stainを使用した場合が,他検出法に比べ高感度・鮮明な反応が得られ,微小抗原に対する染色性が良好であった.抗原量と色素の選択による染色性の検討から,青色のBCIP/NBT,赤色のNew fuchsin,茶色のDABを用いることにより,良好な三重染色標本を得ることが可能となった.発色後に加熱処理を行うことで青色が増強されるため,染色順はBCIP/NBT発色を一番目,もしくは二番目に行い,BCIP/NBT発色後に加熱処理を行う必要があると思われた.本法を用いることによりリンパ節のような抗原同士が近接している抗原検出に際しても鮮明な反応が得られ,同一細胞内における三種類の抗原検出においても判定が容易となると考えられた.封入に関しては,乾燥後非水溶性封入を行うとBCIP/NBTの色素が流出し,判定が困難となるため,水溶性封入が適していると思われた

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記述言語：日本語   出版者・発行元：特定非営利活動法人日本臨床細胞学会  

記述言語：日本語   掲載種別：研究論文（学術雑誌）   出版者・発行元：特定非営利活動法人日本臨床細胞学会  

CiNii Research

記述言語：日本語   出版者・発行元：特定非営利活動法人 日本臨床細胞学会  

<B>目的:</B>穿刺吸引細胞標本における乳管内乳頭腫 (IDP) の細胞学的特徴と細胞異型に乏しい高分化乳癌との鑑別点について解説した.<BR><B>診断:</B>典型的DIPでは散在性細胞は少なく, 腺上皮細胞, 筋上皮細胞, および間質結合織より構成される結合性の高い乳頭状集塊が観察される. また, 壊死を起こしたIDPでは多量の壊死物質が認められるが, 出現する腺上皮細胞は変性核でN/C比も通常のIDPにみられるものと同様である.<BR><B>鑑別:</B>乳頭癌とIDPの鑑別では,(1) 細胞相互, および間質と上皮細胞の結合性,(2) 集塊内の筋上皮細胞の有無,(3) 散在性細胞の細胞異型 (特にクロマチン形態とN/C比) の観察などが, また, 高分化な乳癌とIDPに由来した重積集団の鑑別では (1) 腺腔への細胞極性の有無,(2) 細胞重積の形態,(3) 化生変化の有無などが良・悪性の鑑別のポイントとなる.<BR><B>結語:</B>IDPと高分化乳癌では細胞学的に類似した所見がみられるが, その診断においてはIDPの細胞学的の特徴やIDPと高分化な乳癌との差異を明確に認識した上で診断に臨む必要がある.

DOI： 10.5795/jjscc.42.149

記述言語：日本語  

記述言語：日本語   掲載種別：研究論文（学術雑誌）  

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総ページ数：ix, 183p   記述言語：日本語

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総ページ数：冊   記述言語：日本語

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記述言語：日本語   出版者・発行元：(NPO)日本高血圧学会  

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記述言語：日本語   出版者・発行元：(公社)日本臨床細胞学会  

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記述言語：日本語   出版者・発行元：(公社)日本臨床細胞学会  

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記述言語：英語   出版者・発行元：(一社)日本循環器学会  

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記述言語：英語   出版者・発行元：(一社)日本循環器学会  

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記述言語：英語   出版者・発行元：(一社)日本循環器学会  

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記述言語：日本語   出版者・発行元：(NPO)日本高血圧学会  

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記述言語：日本語   出版者・発行元：(NPO)日本高血圧学会  

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記述言語：日本語   出版者・発行元：国際医療福祉大学学会  

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記述言語：日本語   出版者・発行元：(NPO)生物試料分析科学会  

ゲノムDNA由来上皮成長因子受容体(EGFR)遺伝子とcDNA由来Siglec-E遺伝子を対象に、複数生じたPCR産物の塩基配列を確認する簡易法について報告した。健常人のヒト口腔細胞より、NucleoSpin DNA RapidLyseを用いてゲノムDNAを抽出した。Aire+細胞株由来の1st strand cDNAはRNAiso Plusにてtotal RNAを抽出し、逆転写酵素SuperScript IV Reverse Transcriptaseを用いて合成した。バンド1由来PCR産物では、解読された塩基配列を相同性検索サイト「NCBI/nucleotide BLAST Search」にて検索した。その結果、ヒト7番染色体に座位するEGFRのRefseqGeneが抽出され、目的産物であることが確認された。バンド3も同様に目的産物であることが確認された。バンド2のシーケンスでは複数の波形が雑多に観察され、BLAST SearchではNo Significant Similarity Foundと相同性のある配列は検出されなかった。バンド5由来PCR産物のシーケンスデータでは、Siglec-EのmRNAが抽出され目的産物であることが確認された。本法は低電圧・長時間でのDNA断片の分離操作が必要ではあるが、特殊な装置を必要とせず、汎用試薬で複数のPCRバンドの塩基配列を決定できると考えられた。

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記述言語：日本語   出版者・発行元：ラボ・サービス  

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記述言語：日本語   出版者・発行元：(株)日本臨床社  

J-GLOBAL

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記述言語：日本語   出版者・発行元：日本組織細胞化学会  

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記述言語：日本語   出版者・発行元：神奈川県臨床細胞学会  

背景:濾胞性リンパ腫は、リンパ節に優位に浸潤し多発性リンパ節腫大を生じるが、ときに節外部位に浸潤する場合もある。今回われわれは、耳下腺浸潤が認められた濾胞性リンパ腫の1例を経験したので報告する。症例:61歳女性。左耳下腺内に辺縁不整な30mm大の腫瘤と多発性の頸部リンパ節腫大を認め、耳下腺癌、悪性リンパ腫、vichrow転移が疑われた。耳下腺腫瘤の超音波ガイド下穿刺吸引細胞診では、少数の上皮細胞を背景に核にくびれを有する小型〜中型の異型リンパ球がみられ、Class III"悪性リンパ腫の疑い"と判定された。耳下腺腫瘍摘出術、左頸部リンパ節生検が行われ、濾胞性リンパ腫grade1およびリンパ腫細胞の耳下腺浸潤と診断された。結論:本症例は出現した異型リンパ球の形態、および単調な細胞像により、唾液腺穿刺吸引細胞診にてリンパ球が多数採取される良性病変や、悪性リンパ腫を除く悪性腫瘍性病変との鑑別が可能であった。唾液腺悪性リンパ腫は、原発のほかに近接するリンパ節から浸潤する場合があることから臨床所見を考慮し判定することが重要と考えられた。(著者抄録)

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記述言語：日本語   出版者・発行元：特定非営利活動法人日本臨床細胞学会  

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記述言語：日本語   出版者・発行元：(株)医学書院  

DOI： 10.11477/mf.1543101265

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記述言語：日本語   出版者・発行元：(株)医学書院  

DOI： 10.11477/mf.1543100105

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記述言語：日本語   出版者・発行元：医歯薬出版(株)  

J-GLOBAL

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記述言語：日本語   出版者・発行元：特定非営利活動法人日本臨床細胞学会  

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記述言語：日本語   出版者・発行元：特定非営利活動法人日本臨床細胞学会  

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記述言語：日本語   出版者・発行元：(公社)日本臨床細胞学会  

穿刺吸引細胞標本における乳管内乳頭腫(IDP)の細胞学的特徴と細胞異型に乏しい高分化乳癌との鑑別点について解説した.IDPでは,細胞重積或いは間質を伴う乳頭状集塊の出現など,他臓器の腺癌であれば構造異型としてとらえることができる所見がごく普通にみられる.したがって,乳腺穿刺細胞診をみるにあたっては,IDPの細胞学的特徴やIDPと高分化な乳癌との差異を明確に認識した上で診断する必要がある.又,このことがIDPを過剰診断することによる乳房切除といった不幸な結果を招かないためにも必須の条件といえる

DOI： 10.5795/jjscc.42.149

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記述言語：日本語   出版者・発行元：(一社)日本臨床衛生検査技師会  

J-GLOBAL

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記述言語：日本語   出版者・発行元：(一社)日本臨床衛生検査技師会  

J-GLOBAL

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記述言語：日本語   出版者・発行元：特定非営利活動法人日本臨床細胞学会  

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開催年月日： 2019年9月

記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2019年9月

記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2018年4月

記述言語：日本語   会議種別：口頭発表（一般）  

千葉県臨床検査技師会は千葉県から休日街頭HIV検査の委託事業を受けている。これまでの活動を分析したところ、HIV検査の陽性率が平日に保健所等で行われるHIV即日検査より高いという結果が得られた。また、受検者に対して行ったアンケートからは同性愛者などHIV感染ハイリスクグループへの啓発が必要であることが判明した。本事業活動はHIV感染者早期発見とHIVを主とした性感染症対策の普及啓発活動に有益であるといえる。(著者抄録)

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開催年月日： 2018年3月

記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2015年10月

記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2014年6月

記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2009年3月

記述言語：日本語   会議種別：口頭発表（一般）  

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開催年月日： 2008年8月

記述言語：英語   会議種別：口頭発表（一般）  

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開催年月日： 2004年

記述言語：日本語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：ポスター発表  

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記述言語：英語   会議種別：シンポジウム・ワークショップ パネル（指名）  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：口頭発表（一般）  

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記述言語：英語   会議種別：ポスター発表  

researchmap

記述言語：英語   会議種別：ポスター発表  

researchmap

記述言語：英語   会議種別：口頭発表（一般）  

researchmap

記述言語：英語   会議種別：ポスター発表  

researchmap

記述言語：英語   会議種別：口頭発表（一般）  

researchmap

記述言語：英語   会議種別：口頭発表（一般）  

researchmap

記述言語：英語   会議種別：口頭発表（一般）  

researchmap

記述言語：英語   会議種別：ポスター発表  

researchmap

記述言語：英語   会議種別：口頭発表（一般）  

researchmap

記述言語：英語   会議種別：口頭発表（一般）  

researchmap

記述言語：英語   会議種別：ポスター発表  

researchmap

記述言語：英語   会議種別：ポスター発表  

researchmap