Updated on 2022/04/01

写真a

 
IKEDA Katsuhide
 
Organization
Graduate School of Medicine Associate professor
Graduate School
Graduate School of Medicine
Undergraduate School
School of Health Sciences
Title
Associate professor
External link

Degree 1

  1. 博士(医学) ( 2014.1   昭和大学 ) 

Research Interests 3

  1. 免疫組織細胞化学

  2. 細胞診断検査学

  3. 人体病理学

Research Areas 2

  1. Life Science / Medical systems

  2. Life Science / Human pathology

Research History 5

  1. Nagoya University   Graduate School of Medicine   Associate professor

    2020.4

  2. 名古屋大学医学部   保健学科   准教授

    2020.4

  3. International University of Health and Welfare   Lecturer

    2016.4 - 2020.3

  4. 国立がん研究センター中央病院   病理・臨床検査科

    2011.5 - 2016.3

  5. 昭和大学藤が丘病院   中央臨床検査部・病理診断科

    2000.4 - 2011.4

Education 1

  1. 昭和大学大学院医学研究科

    2006.4 - 2014.1

Professional Memberships 9

  1. 日本臨床細胞学会東海連合会

    2020.4

  2. 愛知県臨床細胞学会

    2020.4

  3. 千葉県臨床細胞学会   理事

    2018.4 - 2020.3

  4. 千葉県臨床検査技師会   理事

    2017.5 - 2021.4

  5. 千葉県細胞検査士会   理事

    2016.4

  6. 日本組織細胞化学会

    2012

  7. The International Academy of Cytology   CMIAC

    2011

  8. 日本臨床衛生検査技師会

    2002

  9. 日本臨床細胞学会   評議員

    2000

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Committee Memberships 1

  1. 千葉県臨床検査技師会   細胞診研究班  

    2018.5 - 2020.4   

 

Papers 35

  1. Characterizing the Effect of Processing Technique and Solution Type on Cytomorphology Using Liquid-Based Cytology. Reviewed International journal

    Katsuhide Ikeda, Wataru Oboshi, Yusuke Hashimoto, Tetsuya Komene, Yoshitaka Yamaguchi, Shouichi Sato, Sayumi Maruyama, Nozomi Furukawa, Nanako Sakabe, Kohzo Nagata

    Acta cytologica   Vol. 66 ( 1 ) page: 55 - 60   2021.10

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

    INTRODUCTION: Liquid-based cytology (LBC) is increasingly used for nongynecologic applications. However, the cytological preparation of LBC specimens is influenced by the processing technique and the preservative used. In this study, the influence of the processing techniques and preservatives on cell morphology was examined mathematically and statistically. METHODS: Cytological specimens were prepared using the ThinPrep (TP), SurePath (SP), and AutoSmear methods, with 5 different preservative solutions. The cytoplasmic and nuclear areas of Papanicolaou-stained specimens were measured for all samples. RESULTS: The cytoplasmic and nuclear areas were smaller in cells prepared using the 2 LBC methods, compared to that prepared using the AutoSmear method, irrespective of the preservative used. The cytoplasmic and nuclear areas of cells prepared using the SP method were smaller than those of cells prepared using the TP method, irrespective of the preservative used. There were fewer differences among the cytoplasmic areas of cells prepared with different preservative solutions using the TP method; however, the cytoplasmic areas of cells prepared using the SP method changed with the preservative solution used. CONCLUSIONS: The most significant difference affecting the cytoplasmic and nuclear areas was the processing technique. The TP method increased the cytoplasmic and nuclear areas, while the methanol-based PreservCyt solution enabled the highest enlargement of the cell. LBC is a superior preparation technique for standardization of the specimens. Our results offer a better understanding of methods suitable for specimen preparation for developing precision AI-based diagnosis in cytology.

    DOI: 10.1159/000519335

    Web of Science

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  2. Periosteum-derived podoplanin-expressing stromal cells regulate nascent vascularization during epiphyseal marrow development. Reviewed

    Tamura S, Mukaide M, Katsuragi Y, Fujii W, Odaira K, Suzuki N, Tsukiji N, Okamoto S, Suzuki A, Kanematsu T, Katsumi A, Takagi A, Ikeda K, Ueyama J, Hirayama M, Suzuki-Inoue K, Matsushita T, Kojima T, Hayakawa F

    The Journal of biological chemistry     page: 101833   2022.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.jbc.2022.101833

    PubMed

  3. The prebiotic fiber inulin ameliorates cardiac, adipose tissue, and hepatic pathology, but exacerbates hypertriglyceridemia in rats with metabolic syndrome Reviewed

    Komatsu Y, Aoyama K, Yoneda M, Ashikawa S, Nakano S, Kawai Y, Cui X, Furukawa N, Ikeda K, Nagata K.

    AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY   Vol. 320 ( 1 ) page: 281 - 295   2021.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1152/ajpheart.00657.2020

  4. Surgical ablation of whitened interscapular brown fat ameliorates cardiac pathology in salt-loaded metabolic syndrome rats Reviewed

    Komatsu Y, Aoyama K, Yoneda M, Ito S, Sano Y, Kawai Y, Cui X, Yamada Y, Furukawa N, Ikeda K, Nagata K.

    ANNALS OF THE NEW YORK ACADEMY OF SCIENCES     2020.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/nyas.14546

  5. MicroRNA-150 suppresses p27 Kip1 expression and promotes cell proliferation in HeLa human cervical cancer cells Reviewed

    Oboshi W, Hayashi K, Takeuchi H, Ikeda K, Yamaguchi Y, Kimura A, Nakamura T, Yukimasa N.

    ONCOLOGY LETTERS   Vol. 20 ( 5 ) page: 210   2020.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3892/ol.2020.12073

  6. Effects of the Storage Solution Type and Prolonged Storage on the Immunoreactivity of Cells. Reviewed

    Ikeda K, Sato S, Chigira H, Shibuki Y, Hiraoka N

    ACTA CYTOLOGICA   Vol. 64 ( 4 ) page: 352 - 359   2020.7

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1159/000503268

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  7. Characterizing the Effect of Automated Cell Sorting Solutions on Cytomorphological Changes. Reviewed

    Ikeda K, Sato S, Chigira H, Shibuki Y, Hiraoka N

    ACTA CYTOLOGICA   Vol. 64 ( 3 ) page: 232 - 240   2020.5

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1159/000500769

    PubMed

  8. Multiple immunoenzyme labeling using heat treatment combined with the polymer method: an analysis of the appropriate inactivation conditions of primary antibodies Reviewed

    Ikeda K, Suzuki T, Tate G, Mitsuya T.

    Acta Histochem   Vol. 113 ( 2 ) page: 117 - 124   2011.2

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.acthis.2009.08.007

  9. A case of dysgerminoma of the ovary with early carcinomatous features Reviewed

      Vol. 51 ( 2 ) page: 269 - 271   2007.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1365-2559.2007.02742.x

    Web of Science

  10. A novel multicolor immunofluorescence method using heat treatment. Reviewed

      Vol. 59 ( 4 ) page: 145 - 51   2005.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.18926/AMO/31955

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  11. Proximal-type epithelioid sarcoma in a 36-year-old man: closer immunoelectron-microscopic resemblance of the tumor cells to epithelial cells than to mesenchymal cells. Reviewed

      Vol. 54 ( 8 ) page: 616 - 22   2004.8

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    DOI: 10.1111/j.1440-1827.2004.01671.x

    PubMed

  12. A modified multicolor immunofluorescence method using boiling technique Reviewed

    SUZUKI Takao, IKEDA Katsuhide, TATE Genshu, MITSUYA Toshiyuki

      Vol. 53 ( 2 ) page: 112 - 116   2004.2

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    Language:Japanese  

  13. An immunoenzyme triple-staining method using highly sensitive indirect technique Reviewed

    IKEDA Katsuhide, SUZUKI Takao, KISHIMOTO Koji, KITAMURA Takashi, MASUNAGA Atsuko, TATE Genshu, MITSUYA Toshiyuki, TAKENAGA Masato

      Vol. 52 ( 7 ) page: 944 - 949   2003.7

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    Authorship:Lead author, Corresponding author   Language:Japanese  

  14. Pharmacological inhibition of the lipid phosphatase PTEN ameliorates heart damage and adipose tissue inflammation in stressed rats with metabolic syndrome. Reviewed International journal

    Sao Ashikawa, Yuki Komatsu, Yumeno Kawai, Kiyoshi Aoyama, Shiho Nakano, Xixi Cui, Misaki Hayakawa, Nanako Sakabe, Nozomi Furukawa, Katsuhide Ikeda, Toyoaki Murohara, Kohzo Nagata

    Physiological reports   Vol. 10 ( 1 ) page: e15165   2022.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Phosphatidylinositol 3-kinase (PI3K) signaling promotes the differentiation and proliferation of regulatory B (Breg) cells, and the lipid phosphatase phosphatase and tensin homolog deleted on chromosome 10 (PTEN) antagonizes the PI3K-Akt signaling pathway. We previously demonstrated that cardiac Akt activity is increased and that restraint stress exacerbates hypertension and both heart and adipose tissue (AT) inflammation in DS/obese rats, an animal model of metabolic syndrome (MetS). We here examined the effects of restraint stress and pharmacological inhibition of PTEN on heart and AT pathology in such rats. Nine-week-old animals were treated with the PTEN inhibitor bisperoxovanadium-pic [bpV(pic)] or vehicle in the absence or presence of restraint stress for 4 weeks. BpV(pic) treatment had no effect on body weight or fat mass but attenuated hypertension in DS/obese rats subjected to restraint stress. BpV(pic) ameliorated left ventricular (LV) inflammation, fibrosis, and diastolic dysfunction as well as AT inflammation in the stressed rats. Restraint stress reduced myocardial capillary density, and this effect was prevented by bpV(pic). In addition, bpV(pic) increased the proportions of Breg and B-1 cells as well as reduced those of CD8+ T and B-2 cells in AT of stressed rats. Our results indicate that inhibition of PTEN by bpV(pic) alleviated heart and AT inflammation in stressed rats with MetS. These positive effects of bpV(pic) are likely due, at least in part, to a reduction in blood pressure, an increase in myocardial capillary formation, and an altered distribution of immune cells in fat tissue that result from the activation of PI3K-Akt signaling.

    DOI: 10.14814/phy2.15165

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  15. Alleviation of salt-induced exacerbation of cardiac, renal, and visceral fat pathology in rats with metabolic syndrome by surgical removal of subcutaneous fat. Reviewed International journal

    Kiyoshi Aoyama, Yuki Komatsu, Mamoru Yoneda, Shiho Nakano, Sao Ashikawa, Yumeno Kawai, Xixi Cui, Katsuhide Ikeda, Kohzo Nagata

    Nutrition & diabetes   Vol. 10 ( 1 ) page: 28 - 28   2020.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    OBJECTIVES: Evidence suggests that visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) should be considered as distinct types of white fat. Although VAT plays a key role in metabolic syndrome (MetS), the role of subcutaneous adipose tissue (SAT) has been unclear. DahlS.Z-Leprfa/Leprfa (DS/obese) rats, an animal model of MetS, develop adipocyte hypertrophy and inflammation to similar extents in SAT and VAT. We have now investigated the effects of salt loading and SAT removal on cardiac, renal, and VAT pathology in DS/obese rats. METHODS: DS/obese rats were subjected to surgical removal of inguinal SAT or sham surgery at 8 weeks of age. They were provided with a 0.3% NaCl solution as drinking water or water alone for 4 weeks from 9 weeks of age. RESULTS: Salt loading exacerbated hypertension, insulin resistance, as well as left ventricular (LV) hypertrophy, inflammation, fibrosis, and diastolic dysfunction in DS/obese rats. It also reduced both SAT and VAT mass but aggravated inflammation only in VAT. Although SAT removal did not affect LV hypertrophy in salt-loaded DS/obese rats, it attenuated hypertension, insulin resistance, and LV injury as well as restored fat mass and alleviated inflammation and the downregulation of adiponectin gene expression in VAT. In addition, whereas salt loading worsened renal injury as well as upregulated the expression of renin-angiotensin-aldosterone system-related genes in the kidney, these effects were suppressed by removal of SAT. CONCLUSIONS: SAT removal attenuated salt-induced exacerbation of MetS and LV and renal pathology in DS/obese rats. These beneficial effects of SAT removal are likely attributable, at least in part, to inhibition of both VAT and systemic inflammation.

    DOI: 10.1038/s41387-020-00132-1

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  16. A case of simultaneous occurrence of double cancer (tongue cancer and breast cancer) as two separate primary tumor

    International journal of analytical bio-science   Vol. 6 ( 4 ) page: 55 - 59   2018.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:The Society of Analytical Bio-Science  

  17. Effusion cytomorphology of small round cell tumors Reviewed

    Katsuhide Ikeda, Koji Tsuta

    JOURNAL OF CYTOLOGY   Vol. 33 ( 2 ) page: 85 - 92   2016

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:MEDKNOW PUBLICATIONS & MEDIA PVT LTD  

    Background: Small round cell tumors (SRCTs) are a group of tumors composed of small, round, and uniform cells with high nuclear/cytoplasmic (N/C) ratios. The appearance of SRCT neoplastic cells in the effusion fluid is very rare. We reported the cytomorphological findings of SRCTs in effusion cytology, and performed statistical and mathematical analyses for a purpose to distinguish SRCTs.
    Materials and Methods: We analyzed the cytologic findings of effusion samples from 40 SRCT cases and measured the lengths of the nuclei, cytoplasms, and the cell cluster areas. The SRCT cases included 14 Ewing sarcoma (EWS)/primitive neuroectodermal tumor cases, 5 synovial sarcoma cases, 6 rhabdomyosarcoma cases, 9 small cell lung carcinoma (SCLC) cases, and 6 diffuse large B-cell lymphoma (DLBL) cases.
    Results: Morphologically, there were no significant differences in the nuclear and cytoplasmic lengths in cases of EWS, synovial sarcoma, and rhabdomyosarcoma. The cytoplasmic lengths in cases of SCLC and DLBL were smaller than those of EWS, synovial sarcoma, and rhabdomyosarcoma. The nuclear density of the cluster in SCLC was higher than that in other SRCTs, and cases of DLBL showed a lack of anisokaryosis and anisocytosis.
    Conclusion: We believe that it might be possible to diagnose DLBL and SCLC from cytologic analysis of effusion samples but it is very difficult to use this method to distinguish EWS, synovial sarcoma, and rhabdomyosarcoma. Statistical and mathematical analyses indicated that nuclear density and dispersion of nuclear and cytoplasmic sizes are useful adjuncts to conventional cytologic diagnostic criteria, which are acquired from experience.

    DOI: 10.4103/0970-9371.182529

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  18. Wide local extension and higher proliferation indices are characteristic features of symptomatic lobular neoplasias (LNs) and LNs with an early invasive component. Reviewed International journal

    Yuka Katsurada, Masayuki Yoshida, Akiko Miyagi Maeshima, Katsuhide Ikeda, Tatsuhiro Shibata, Takayuki Kinoshita, Osamu Matsubara, Hitoshi Tsuda

    Histopathology   Vol. 64 ( 7 ) page: 994 - 1003   2014.6

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    AIMS: Lobular neoplasias (LNs) are typically small, clinically undetectable breast lesions, but some LNs are of clinical significance. The aim of this study was to clarify the histopathological characteristics of clinically overt (symptomatic) LNs and early invasive LNs. METHODS AND RESULTS: Sixty-two surgically resected LNs, including eight with early invasion (≤10 mm), were classified into the following groups: (i) symptomatic and occult; and (ii) early invasive and non-invasive. Six histopathological factors, including the Ki67 labelling index (LI), were assessed and analysed by logistic regression models. On multivariate analysis, tumour size (P = 0.008), mitotic counts (P = 0.006) and Ki67 LI (P = 0.035) were risk factors for symptomatic features, and tumour size (P = 0.009) and Ki67 LI (P = 0.015) were risk factors for early invasive lesions. In the eight LNs with invasion, the symptomatic and occult subgroups showed differing nuclear atypia and structural patterns, but both lesions extended widely (22-96 mm). CONCLUSIONS: Wide extension and higher proliferation activity were characteristic features of symptomatic LNs and LNs with early invasion.

    DOI: 10.1111/his.12356

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  19. Diagnostic accuracy of fine-needle aspiration cytology of the breast in Japan: report from the Working Group on the Accuracy of Breast Fine-Needle Aspiration Cytology of the Japanese Society of Clinical Cytology. Reviewed International journal

    Rin Yamaguchi, Shin-ιchi Tsuchiya, Takashi Koshikawa, Akinori Ishihara, Shinobu Masuda, Ichiro Maeda, Masafumi Takimoto, Masashi Kawamoto, Haruaki Satoh, Michihiko Narita, Hitoshi Itoh, Takashi Kitamura, Yoshiko Tsuda, Naoki Ogane, Eiji Abe, Katsuhide Ikeda, Takeshi Nakamura, Harumi Kamaguchi, Yoshiro Tokoro

    Oncology reports   Vol. 28 ( 5 ) page: 1606 - 12   2012.11

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPANDIDOS PUBL LTD  

    The Working Group of the Japanese Society of Clinical Cytology was assembled to assess the current status of breast cytology in Japan by conducting a large-scale survey regarding the accuracy of fine-needle aspiration biopsy (FNAB) in Japan. We collected data and investigated the status of breast cytological diagnosis at 12 different cooperating facilities in Japan, and re-evaluated their false-negative and false-positive cases. Among 30,535 individuals who underwent a breast cytological examination, analyses were conducted on 10,890 individuals (35.7%) in whom cytological diagnoses were confirmed by histology. Among these patients, the cytological diagnosis had an inadequate rate of 17.7%, an indeterminate rate of 7.8%, a positive predictive value of 'malignancy suspected' cells of 92.4%, an absolute sensitivity of 76.7%, a complete sensitivity of 96.7%, a specificity of 84.3%, a positive predictive value of 'malignant' cells of 99.5%, a false-negative value of 3.31%, a false-positive value of 0.25% and an accuracy rate of 88.0%. Subsequently, 297 false-negative and 23 false-positive cases were re-evaluated and several factors were characterized (i.e. histological type, tumor size and misread points). This survey collected data from a large number of cases for breast FNAB. Based on our survey, the accuracy of FNAB in Japan was relatively high compared with the goal of assessment of diagnostic accuracy. However, there were some false-negative and false-positive cases. Improvements in accuracy resulting from the learning points in the present study will lead to more useful and reliable diagnostic tools in clinical practice.

    DOI: 10.3892/or.2012.2014

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  20. Comparison of immunocytochemical sensitivity between formalin-fixed and alcohol-fixed specimens reveals the diagnostic value of alcohol-fixed cytocentrifuged preparations in malignant effusion cytology. Reviewed International journal

    Katsuhide Ikeda, Genshu Tate, Takao Suzuki, Toshiyuki Mitsuya

    American journal of clinical pathology   Vol. 136 ( 6 ) page: 934 - 942   2011.12

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC CLINICAL PATHOLOGY  

    The most commonly used fixative in effusion cytology is formalin. In the present study, the immunocytochemical properties of formalin-fixed and alcohol-fixed specimens were compared to evaluate the usefulness of alcohol-fixed cytocentrifuged preparations for routine cytologic diagnosis. A total of 269 effusion samples and 17 primary antibodies were used. The sensitivity of immunocytochemical studies in alcohol-fixed specimens was similar and correlated to that of formalin-fixed specimens, suggesting that alcohol-fixed cytocentrifuged preparations are useful in effusion cytology. Pretreatment with or without heat-induced antigen retrieval revealed that antigen retrieval was unnecessary for immunocytochemical studies with most primary antibodies in alcohol-fixed cytocentrifuged preparations. The present study describes the use of immunocytochemical studies with alcohol-fixed cytocentrifuged preparations for diagnosis in routine effusion cytology.

    DOI: 10.1309/AJCPAH5WR2LFTUSI

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  21. Diagnostic usefulness of EMA, IMP3, and GLUT-1 for the immunocytochemical distinction of malignant cells from reactive mesothelial cells in effusion cytology using cytospin preparations. Reviewed International journal

    Katsuhide Ikeda, Genshu Tate, Takao Suzuki, Takashi Kitamura, Toshiyuki Mitsuya

    Diagnostic cytopathology   Vol. 39 ( 6 ) page: 395 - 401   2011.6

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:WILEY  

    To differentiate reactive mesothelial cells (RMs) from metastatic carcinoma and malignant mesothelioma (MM) in effusion cytology is crucial for the cytologic diagnosis and the management of the patients. In the present study, the immunocytochemical staining profile of the epithelial membrane antigen (EMA), the insulin-like growth factor-II mRNA-binding protein 3 (IMP3), and the glucose transporter-1 (GLUT-1) was examined to distinguish RMs from malignant cells. A total of 171 pleural (n = 87) and peritoneal (n = 84) effusion specimens, including 50 benign effusions with RMs, 11 MM effusions, and 110 metastatic malignant effusions, were evaluated for immunocytochemistry. EMA, IMP3, monoclonal GLUT-1, and polyclonal GLUT-1 immunoreactivity were observed in 26.0%, 6.0%, 20.0%, and 18.0% of RMs, respectively. In contrast to RMs, the immunoreactivity in MM was 100%, 36.4%, 100%, and 90.9%; adenocarcinoma (AC) was 100%, 80.8%, 81.7%, and 72.1%; squamous-cell carcinoma was 83.3%, 83.3%, 83.3%, and 66.7%. EMA, IMP3, mGLUT-1, and pGLUT-1 expressions were observed in 98.4%, 65.6%, 88.5%, and 75.4% in the pleural effusion with malignant cells, and 100%, 88.3%, 78.3%, and 71.7% in ascites containing malignant cells, respectively. The findings of the present study indicate that the immunocytochemical staining for EMA, IMP3, and GLUT-1 is a useful diagnostic tool for distinguishing effusions containing malignant cells from those that contain benign cells, and in particular, we suggest that the combination of mGLUT-1 and EMA, and IMP3 and EMA are extremely useful in pleural effusion and in ascites, respectively.

    DOI: 10.1002/dc.21398

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  22. 耳下腺基底細胞腺腫の2例

    池田勝秀, 北村隆司, 鈴木孝夫, 田尻琢磨, 増永敦子, 楯玄秀, 光谷俊幸

    日本臨床細胞学会神奈川県支部会誌   Vol. 15 ( 1 ) page: 54 - 59   2010.7

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

    J-GLOBAL

  23. IMP3/L523S, a novel immunocytochemical marker that distinguishes benign and malignant cells: the expression profiles of IMP3/L523S in effusion cytology. Reviewed International journal

    Katsuhide Ikeda, Genshu Tate, Takao Suzuki, Takashi Kitamura, Toshiyuki Mitsuya

    Human pathology   Vol. 41 ( 5 ) page: 745 - 750   2010.5

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    Differentiating reactive mesothelial cells from metastatic carcinoma and malignant mesothelioma is critical in effusion cytology. Numerous immunohistochemical/cytochemical reports use various antibodies in effusion samples, and most antibodies differentiate metastatic adenocarcinoma from malignant mesothelioma, but no antibodies help distinguish malignant mesothelioma from reactive mesothelial cells. A mouse monoclonal antibody (IMP3/L523S) against KOC is a 580-amino acid oncofetal RNA-binding protein containing 4 K homology domains. IMP3/L523S has been identified in several human malignant tumors. The immunocytochemical staining profile of IMP3 was determined in 95% alcohol-fixed cytologic effusion specimens. A total of 229 cases of pleural and peritoneal effusion cytospecimens were evaluated for the study, including 39 benign effusions with reactive mesothelial cells and 190 metastatic malignant effusions. IMP3 immunoreactivity was observed in 2 (5.1%) of 39 cases of reactive mesothelial cells, 138 (72.6%) of 190 cases of malignant effusion, 4 (36.4%) of 11 cases of malignant mesothelioma, 106 (75.7%) of 140 cases of metastatic adenocarcinoma, and 8 (100%) of 8 cases of squamous cell carcinoma. The overall specificity for the diagnosis of malignancy was 94.9%, whereas the sensitivity was 72.6%. In the peritoneal effusions, the sensitivity for the diagnosis of metastatic adenocarcinoma to distinguish reactive mesothelial cells was 92.3%. In conclusion, IMP3 staining is present in many carcinomas and is not a useful marker for distinguishing between carcinomas arising in different organs. However, the IMP3 antibody is a highly specific marker for malignant lesions, and thus, IMP3 staining is useful for distinguishing neoplastic cells from reactive mesothelial cells in effusion samples.

    DOI: 10.1016/j.humpath.2009.04.030

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  24. Effusion cytomorphology and immunocytochemistry of malignant melanoma: five cases of melanotic melanoma and one case of amelanotic melanoma. Reviewed International journal

    Katsuhide Ikeda, Genshu Tate, Keiichi Iezumi, Takao Suzuki, Takashi Kitamura, Toshiyuki Mitsuya

    Diagnostic cytopathology   Vol. 37 ( 7 ) page: 516 - 521   2009.7

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    Effusion cytological analyses of amelanotic malignant melanoma (AMM) are very rare and no concise description of AMM related cytomorphologic features using effusion have been reported. Here, we report the cytomorphological, immunohistochemical, and immunocytochemical findings in the effusion cytology of six cases of malignant melanoma (MM), one case of AMM, and five cases of melanotic malignant melanoma. Papanicolaou-stained smears exhibited conspicuous nucleoli, multinucleation, and cytoplasmic vacuolization in all of the MM cases. In addition, the AMM case displayed numerous mitotic figures and intranuclear cytoplasmic inclusions. With regard to the immunohistochemistry findings, all six cases of melanoma were positive for Melan-A/MART-1, HBME-1, and S-100. In the immunohistochemistry analyses, five of six cases of melanoma were positive for WT-1, as was the AMM specimen. Furthermore, because the effusion analysis of malignant mesothelioma proved positive for WT-1, it should be noted that WT-1 effusion analysis is not an appropriate means to distinguish between MM and malignant mesothelioma. We suggest that it is important to recognize cytomorphologic characteristics, such as melanin pigment, conspicuous nucleoli, multinucleation, and cytoplasmic vacuolization, and to choose appropriate antibodies for the correct diagnosis of MM in effusion.

    DOI: 10.1002/dc.21054

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  25. Cytomorphologic features of well-differentiated papillary mesothelioma in peritoneal effusion: a case report. Reviewed International journal

    Katsuhide Ikeda, Takao Suzuki, Genshu Tate, Toshiyuki Mitsuya

    Diagnostic cytopathology   Vol. 36 ( 7 ) page: 512 - 515   2008.7

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

    Well-differentiated papillary mesothelioma (WDPM), a distinct subtype of diffuse malignant mesothelioma, usually occurs in the peritoneum and is seen most commonly in women of reproductive age. Histologic features of WDPM include papillary growth and stout fibrous cores surrounded by a single layer of tumor cells. We present the case of a 73-year-old woman without subjective symptoms who showed signs of peritoneal effusion during a routine examination and for whom cytologic examination of the ascitic fluid was performed. Many spherical clusters, with a smooth external surface composed of a single layer of uniform cuboidal cells, were observed. Within each cluster, a collagenous ball showed light green Papanicolaou staining. Immunohistochemistry of surgical specimens showed tumor cells positive for calretinin, D(2)-40, and HBME-1 staining. The histologic diagnosis was WDPM. The identification of a collagenous ball within these clusters is a useful cytologic finding for the diagnosis of WDPM. WDPM should be suspected when numerous collagenous balls are present by effusion cytology and isolated cells are not.

    DOI: 10.1002/dc.20824

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  26. Coordinate expression of cytokeratin 8 and cytokeratin 17 immunohistochemical staining in cervical intraepithelial neoplasia and cervical squamous cell carcinoma: an immunohistochemical analysis and review of the literature. Reviewed International journal

    Katsuhide Ikeda, Genshu Tate, Takao Suzuki, Toshiyuki Mitsuya

    Gynecologic oncology   Vol. 108 ( 3 ) page: 598 - 602   2008.3

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

    OBJECTIVE: Several Cytokeratin (CK) isoforms have been analyzed in cervical intraepithelial lesions. However, previously reported numbers of specimens have been too low to evaluate any correlation between CK and CIN. METHODS: We examined the immunohistochemical staining of p16, CK8, and CK17 in 134 cervical tissues obtained by punch biopsy and graded as follows: CIN I (n=39), CIN II (n=31), CIN III (n=43), SCC (n=21). RESULTS: p16 staining was identified in 74.4% of CIN I, 93.6% of CIN II, 97.7% of CIN III, and 100% of SCC cases. CK8 and CK17 staining were identified in 12.8% and 33.3% of CIN I, 22.6% and 58.1% of CIN II, 62.8% and 81.4% of CIN III, and 71.4% and 95.2% of SCC cases, respectively. Interestingly, positivity for CK8 and CK17 correlated with increasing lesion grade of the intraepithelial lesions and also correlated with p16 staining (p16, p=0.0008; CK8, p<0.0001, and CK17, p<0.0001), and a coordinate expression profile of CK8[+]/CK17[+] correlates with increasing CIN grade and carcinoma (likewise, a coordinate expression profile of CK8[-]/CK17[-] correlates with decreasing CIN grade and absence of carcinoma), but expression of just CK8 (CK8[+]/CK17[-]) or just CK17 (CK8[-]/CK17[+]) does not correlate with increasing CIN grade and carcinoma. CONCLUSIONS: Results of the present study showed that p16, CK8, and CK17 immunostaining differed according to the degree of cervical intraepithelial lesions and SCC, and surprisingly, that staining was significantly correlated with increasing lesion grade of CIN and SCC.

    DOI: 10.1016/j.ygyno.2007.11.042

    Web of Science

    PubMed

  27. Clear cell adenocarcinoma arising from a giant cystic adenomyosis: a case report with immunohistochemical analysis of laminin-5 gamma2 chain and p53 overexpression. Reviewed International journal

    Yoshiki Ohta, Shigeharu Hamatani, Takao Suzuki, Katsuhide Ikeda, Kaoru Kiyokawa, Akira Shiokawa, Miki Kushima, Hidekazu Ota

    Pathology, research and practice   Vol. 204 ( 9 ) page: 677 - 682   2008

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    We report a case of a clear cell adenocarcinoma arising from a giant cystic adenomyosis, with immunohistochemical analysis of p53 and laminin-5 gamma2 chain overexpression. Microscopically, not only clear cell adenocarcinoma showing myometrial invasion but also single-layered clear cell adenocarcinoma cells lining the cyst wall were observed. Transition from these single-layered tumor cells to papillary proliferative lesions of various degrees was recognized. Moreover, these tumor cells were continuous with minimal atypical cells. Although the tumor cells within the uterus showed a low positive cell ratio for p53, the metastatic foci showed a remarkable p53 overexpression. Laminin-5 gamma2 chain expression was low in papillary proliferation and high in myometrial invasion and metastatic foci. The single-layered tumor cells showing non-invasive proliferation also contained laminin-5 gamma2 chain-positive cells. When non-invasive tumor cells were considered to be at an early stage in tumor progression, some tumor cells had already acquired an invasive feature. p53 overexpression was not related to expression of the laminin-5 gamma2 chain.

    DOI: 10.1016/j.prp.2008.02.005

    Web of Science

    PubMed

  28. Cytologic comparison of a primary parathyroid cancer and its metastatic lesions: a case report. Reviewed International journal

    Katsuhide Ikeda, Genshu Tate, Takao Suzuki, Toshiyuki Mitsuya

    Diagnostic cytopathology   Vol. 34 ( 1 ) page: 50 - 55   2006.1

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    We describe the fine-needle aspiration cytology features of a primary parathyroid cancer and of the local recurrent and distant metastatic lesions. The presence of prognostic factors Ki-67 and proliferating cell nuclear antigen (PCNA) was compared immunohistochemically between primary parathyroid carcinoma and related metastatic and recurrent foci. Flow cytometric DNA analysis was also performed to investigate any chromosomal abnormality of the parathyroid carcinoma. Cytologic examination of the endocrine tumor showed that it comprised a loose cohesive cluster and tumor cells with granular cytoplasm and mild nuclear atypia, but for purposes of cytodiagnosis, it is difficult to determine whether such a neoplasm is malignant on the basis of morphology alone. Immunohistochemical analysis showed that Ki-67 and PCNA labeling indices were higher in the recurrent and metastasized carcinomas than in the primary cancer, suggesting that neoplastic cells become more malignant in the recurrent and metastasized foci. To our knowledge, this is the first report describing not only cytopathologic but also immunocytologic differences between primary parathyroid cancer and the metastatic lesion.

    DOI: 10.1002/dc.20399

    Web of Science

    PubMed

  29. Cytomorphologic features of immature ovarian teratoma in peritoneal effusion: a case report. Reviewed International journal

    Katsuhide Ikeda, Genshu Tate, Takao Suzuki, Toshiyuki Mitsuya

    Diagnostic cytopathology   Vol. 33 ( 1 ) page: 39 - 42   2005.7

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    We describe unusual cytologic features of the peritoneal fluid in a patient with immature ovarian teratoma. Immature embryonal and neuroectodermal components of such tumors are rarely observed in the ascites; to our knowledge, there have been only three reports of cytologic findings of immature teratoma cells in the ascites. Our patient was a 26-yr-old woman who presented with a huge pelvic mass. A grade 3 immature right ovarian teratoma was diagnosed pathologically. Cytologic examination of the ascitic fluid revealed a variety of neoplastic cells, including immature neuroepithelial cells forming rosette-like structures, keratinized squamous cells, squamoid metaplastic cells, and immature glial-appearing cells. Immunohistochemical analysis revealed that immature gastrointestinal elements and yolk sac elements were positive for alpha-fetoprotein. This is the first description of the cytologic features of non-neuroepithelial elements in the ascitic fluid in a patient with immature ovarian teratoma.

    DOI: 10.1002/dc.20259

    Web of Science

    PubMed

  30. Effusion cytodiagnosis of carcinosarcoma derived from the female genital tract: immunohistochemical features of MMP-7 and Ki-67 and immunofluorescence double staining analyses of eight cases. Reviewed International journal

    Katsuhide Ikeda, Genshu Tate, Takao Suzuki, Toshiyuki Mitsuya

    Gynecologic oncology   Vol. 97 ( 2 ) page: 323 - 329   2005.5

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    OBJECTIVE: Expression of Matrix metalloproteinase 7 (MMP-7) and Ki-67 by carcinoma components (CCs) and sarcoma components (SCs) in carcinosarcoma of the female reproductive organs has been investigated by conventional methods, but analysis with immunohistochemical staining of multiple antigens has not been reported. We report the profiles of expression of MMP-7 and Ki-67 in carcinosarcoma determined with immunohistochemical staining techniques. METHODS: We used antibodies against epithelial antigen (EA), epithelial membrane antigen (EMA), and vimentin for immunofluorescence double staining of ascitic fluid in eight cases of carcinosarcoma of female reproductive organs. We also used immunohistochemical triple staining to compare MMP-7 and Ki-67 expression between CCs and SCs in the primary site of carcinosarcoma. RESULTS: Immunofluorescence analysis revealed that all neoplastic cells in the ascitic fluid were positive for EA or EMA, indicating that these cells were CCs. Immunohistochemical analyses of the primary organ of carcinosarcoma revealed that MMP-7 was expressed on CCs in four of eight cases of carcinosarcoma, whereas MMP-7 was not expressed on SCs. The average Ki-67 labeling index (LI) in CCs and SCs was 51.8% and 28.6%, respectively. The difference in Ki-67 LI between CCs and SCs was statistically significant (t test for paired samples, P = 0.0173). CONCLUSIONS: This is the first study to examine carcinosarcoma of the female reproductive organ by immunohistochemical staining for multiple antigens, which allows analysis of mixed tumor elements. In addition, we found that expression of MMP-7 and the average Ki-67 LI differ between CCs and SCs in carcinosarcoma. The predominance of CCs as the malignant cells in the ascitic fluid may be due to cytological differences between CCs and SCs of carcinosarcoma.

    DOI: 10.1016/j.ygyno.2004.12.024

    Web of Science

    PubMed

  31. Fine needle aspiration cytology of primary proximal-type epithelioid sarcoma of the perineum: a case report. Reviewed

    Katsuhide Ikeda, Genshu Tate, Takao Suzuki, Toshiyuki Mitsuya

    Acta cytologica   Vol. 49 ( 3 ) page: 314 - 8   2005.5

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1159/000326155

    PubMed

  32. An immunoenzyme triple-staining method using highly sensitive indirect technique

    池田勝秀, 鈴木孝夫, 岸本浩次, 北村隆司, 増永敦子, 楯玄秀, 光谷俊幸, 武永正人

    医学検査   Vol. 52 ( 7 ) page: 944 - 949   2003.7

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    J-GLOBAL

  33. A benign disease of the breast which raises the possibility of positive diagnosis : Intraductal papilloma

    KITAMURA Takashi, NASU Naomi, IKEDA Katsuhide, MASUNAGA Atsuko, TATE Genshu, MITSUYA Toshiyuki, WATANABE Tadashi, OTA Hidekazu

    The Journal of the Japanese Society of Clinical Cytology   Vol. 42 ( 2 ) page: 149 - 154   2003.3

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  34. A benign disease of the breast which raises the possibility of positive diagnosis-Intraductal papilloma-:Intraductal papilloma

    KITAMURA Takashi, NASU Naomi, IKEDA Katsuhide, MASUNAGA Atsuko, TATE Genshu, MITSUYA Toshiyuki, WATANABE Tadashi, OTA Hidekazu

    The Journal of the Japanese Society of Clinical Cytology   Vol. 42 ( 2 ) page: 149 - 154   2003

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    Language:Japanese   Publisher:The Japanese Society of Clinical Cytology  

    <B><I>Purpose</I>:</B> Cytologic features of intraductal papilloma (IDP) and differential diagnosis between IDP and well-differentiated breast carcinoma.<BR><I>Diagnosis</I>: Typical IDP shows papillary and cohesive clusters composed of duct epithelium, myoepithelium, and stroma, and discohesive cells are rare in the background. In IDP with necrosis, necrotic debris is massive and epithelial cells show degenerated nuclei, but there are no differences in the N/C ratio between IDP cells and normal columnar epithelia.<BR><B><I>Differential Diagnosis</I>:</B> To differentiate IDP from papillary carcinoma, it is important to observe 1) cohesiveness among epithelial cells and between epithelial cells and stromal tissues; 2) presence or absence of myoepithelial cells in clusters ; and 3) cytologic atypia of discohesive clusters (in particular, the features of chromatin and N/C ratio). Important differential diagnostic considerations are to distinguish cohesive clusters of IDP from those of well-differentiated breast cancer, it is important to pay attentions to 1) cellular polarity toward the lumen, 2) cohesiveness of clusters, and 3) presence or absence of metaplastic changes.<BR><B><I>Conclusion</I>:</B> Because cytological features of IDP are quite similar to those of well-differentiated breast carcinoma, it is important for correct diagnosis to keep in mind and image cytological characteristics of IDP and cytologic differences from welldifferentiated breast carcinoma.

    DOI: 10.5795/jjscc.42.149

  35. An autopsy case of extrapancreatic insulinoma in an elderly patient with symptoms resembling senile dementia Reviewed

    TAJIRI Takuma, TATE Genshu, KUNIMURA Toshiaki, SUZUKI Seiji, MITSUYA Toshiyuki, MOROHOSHI Toshio

      Vol. 17 ( 5 ) page: 500 - 505   2002.10

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Books 4

  1. 染色法のすべて

    ( Role: Contributor ,  レフレルのメチレン青染色)

    医歯薬出版  2021.3 

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    Language:Japanese Book type:Textbook, survey, introduction

  2. 組織細胞化学2019

    ( Role: Contributor ,  免疫染色の応用 -多重染色法-)

    学際企画  2019.7 

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    Language:Japanese

  3. 実践から学ぶ 婦人科細胞診

    ( Role: Contributor)

    医療科学社  2017.6 

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    Language:Japanese

  4. 組織細胞化学2012

    ( Role: Contributor ,  免疫組織化学多重染色法)

    学際企画  2012.7 

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    Language:Japanese

MISC 12

  1. ゲノムDNAにおける簡易抽出法の開発について

    橋本 優佑, 米根 鉄矢, 大星 航, 池田 勝秀, 山口 良考

    国際医療福祉大学学会誌   Vol. 25 ( 抄録号 ) page: 118 - 118   2020.11

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    Language:Japanese   Publisher:国際医療福祉大学学会  

  2. 複数生じたPCRバンドの簡易シーケンス法

    中村 蓮, 北村 浩一, 京都 敬祐, 藤井 樹, 岩淵 由起, 橋本 優佑, 米根 鉄矢, 池田 勝秀, 大星 航, 平田 雄哉, 飯塚 信義, 古賀 豊大, 田嶋 明彦, 山口 良考

    生物試料分析   Vol. 43 ( 3 ) page: 200 - 207   2020.6

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    Language:Japanese   Publisher:(NPO)生物試料分析科学会  

    ゲノムDNA由来上皮成長因子受容体(EGFR)遺伝子とcDNA由来Siglec-E遺伝子を対象に、複数生じたPCR産物の塩基配列を確認する簡易法について報告した。健常人のヒト口腔細胞より、NucleoSpin DNA RapidLyseを用いてゲノムDNAを抽出した。Aire+細胞株由来の1st strand cDNAはRNAiso Plusにてtotal RNAを抽出し、逆転写酵素SuperScript IV Reverse Transcriptaseを用いて合成した。バンド1由来PCR産物では、解読された塩基配列を相同性検索サイト「NCBI/nucleotide BLAST Search」にて検索した。その結果、ヒト7番染色体に座位するEGFRのRefseqGeneが抽出され、目的産物であることが確認された。バンド3も同様に目的産物であることが確認された。バンド2のシーケンスでは複数の波形が雑多に観察され、BLAST SearchではNo Significant Similarity Foundと相同性のある配列は検出されなかった。バンド5由来PCR産物のシーケンスデータでは、Siglec-EのmRNAが抽出され目的産物であることが確認された。本法は低電圧・長時間でのDNA断片の分離操作が必要ではあるが、特殊な装置を必要とせず、汎用試薬で複数のPCRバンドの塩基配列を決定できると考えられた。

    Other Link: https://search.jamas.or.jp/index.php?module=Default&action=Link&pub_year=2020&ichushi_jid=J01925&link_issn=&doc_id=20200709020007&doc_link_id=%2Fcu8analy%2F2020%2F004303%2F008%2F0200-0207%26dl%3D0&url=http%3A%2F%2Fwww.medicalonline.jp%2Fjamas.php%3FGoodsID%3D%2Fcu8analy%2F2020%2F004303%2F008%2F0200-0207%26dl%3D0&type=MedicalOnline&icon=https%3A%2F%2Fjk04.jamas.or.jp%2Ficon%2F00004_2.gif

  3. 臨床検査カレッジ:細胞学的検討(2)神経芽腫群腫瘍の細胞学的検討

    福留伸幸, 池田勝秀, 水口國雄

    医療と検査機器・試薬   Vol. 37 ( 6 ) page: 741 - 752   2014.12

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    J-GLOBAL

  4. 肺癌の検査・診断 検査・診断 病理検査・診断 液状検体からのセルブロック作製の意義と方法

    池田勝秀, 蔦幸治

    日本臨床   Vol. 71 ( 増刊6 最新肺癌学 ) page: 404 - 407   2013.11

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    J-GLOBAL

  5. 免疫組織化学多重染色法(A.染める!-免疫染色法の基礎と応用-,組織細胞化学の挑戦-臨床応用研究への飛躍)

    鈴木 孝夫, 池田 勝秀, 柳田 絵美衣

    組織細胞化学   Vol. 2012   page: 35 - 47   2012.7

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    CiNii Books

  6. 耳下腺浸潤が認められた濾胞性リンパ腫の1例

    佐々木 陽介, 北村 隆司, 池田 勝秀, 岸本 浩次, 楯 玄秀, 光谷 俊幸, 太田 秀一

    日本臨床細胞学会神奈川県支部会誌   Vol. 16 ( 1 ) page: 70 - 76   2011.7

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    Language:Japanese   Publisher:神奈川県臨床細胞学会  

    背景:濾胞性リンパ腫は、リンパ節に優位に浸潤し多発性リンパ節腫大を生じるが、ときに節外部位に浸潤する場合もある。今回われわれは、耳下腺浸潤が認められた濾胞性リンパ腫の1例を経験したので報告する。症例:61歳女性。左耳下腺内に辺縁不整な30mm大の腫瘤と多発性の頸部リンパ節腫大を認め、耳下腺癌、悪性リンパ腫、vichrow転移が疑われた。耳下腺腫瘤の超音波ガイド下穿刺吸引細胞診では、少数の上皮細胞を背景に核にくびれを有する小型〜中型の異型リンパ球がみられ、Class III"悪性リンパ腫の疑い"と判定された。耳下腺腫瘍摘出術、左頸部リンパ節生検が行われ、濾胞性リンパ腫grade1およびリンパ腫細胞の耳下腺浸潤と診断された。結論:本症例は出現した異型リンパ球の形態、および単調な細胞像により、唾液腺穿刺吸引細胞診にてリンパ球が多数採取される良性病変や、悪性リンパ腫を除く悪性腫瘍性病変との鑑別が可能であった。唾液腺悪性リンパ腫は、原発のほかに近接するリンパ節から浸潤する場合があることから臨床所見を考慮し判定することが重要と考えられた。(著者抄録)

  7. Fluorescent antibody technique multiple dyeing

    鈴木孝夫, 池田勝秀

    検査と技術   Vol. 34 ( 13 ) page: 1477 - 1483   2006.12

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    DOI: 10.11477/mf.1543101265

    J-GLOBAL

  8. Enzyme antibody method triple staining.

    池田勝秀, 鈴木孝夫, 光谷俊幸

    検査と技術   Vol. 33 ( 9 ) page: 803 - 808   2005.9

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    DOI: 10.11477/mf.1543100105

    J-GLOBAL

  9. 熱湯処理を用いた免疫組織化学多重染色法

    鈴木孝夫, 池田勝秀

    Med Technol   Vol. 32 ( 12 ) page: 1298 - 1301   2004.12

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    J-GLOBAL

  10. A benign disease of the breast which raises the possibility of positive diagnosis-Intraductal papilloma-

    北村隆司, 那須直美, 池田勝秀, 増永敦子, 楯玄秀, 光谷俊幸, 渡辺ただし, 太田秀一

    日本臨床細胞学会雑誌   Vol. 42 ( 2 ) page: 149 - 154   2003.3

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    Language:Japanese   Publisher:The Japanese Society of Clinical Cytology  

    <B><I>Purpose</I>:</B> Cytologic features of intraductal papilloma (IDP) and differential diagnosis between IDP and well-differentiated breast carcinoma.<BR><I>Diagnosis</I>: Typical IDP shows papillary and cohesive clusters composed of duct epithelium, myoepithelium, and stroma, and discohesive cells are rare in the background. In IDP with necrosis, necrotic debris is massive and epithelial cells show degenerated nuclei, but there are no differences in the N/C ratio between IDP cells and normal columnar epithelia.<BR><B><I>Differential Diagnosis</I>:</B> To differentiate IDP from papillary carcinoma, it is important to observe 1) cohesiveness among epithelial cells and between epithelial cells and stromal tissues; 2) presence or absence of myoepithelial cells in clusters ; and 3) cytologic atypia of discohesive clusters (in particular, the features of chromatin and N/C ratio). Important differential diagnostic considerations are to distinguish cohesive clusters of IDP from those of well-differentiated breast cancer, it is important to pay attentions to 1) cellular polarity toward the lumen, 2) cohesiveness of clusters, and 3) presence or absence of metaplastic changes.<BR><B><I>Conclusion</I>:</B> Because cytological features of IDP are quite similar to those of well-differentiated breast carcinoma, it is important for correct diagnosis to keep in mind and image cytological characteristics of IDP and cytologic differences from welldifferentiated breast carcinoma.

    DOI: 10.5795/jjscc.42.149

    CiNii Research

    J-GLOBAL

  11. 腎腫ようの細胞診 〔2〕 腎細胞癌の細胞像

    北村隆司, 池田勝秀, 岸本浩次, 光谷俊幸, 太田秀一

    医学検査   Vol. 51 ( 6 ) page: 933 - 935   2002.6

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    J-GLOBAL

  12. 自分のライブラリーを創ろう 腎腫ようの細胞診 (1) 腎細胞癌の細胞像

    北村隆司, 池田勝秀, 岸本浩次, 光谷俊幸, 太田秀一

    医学検査   Vol. 51 ( 5 ) page: 740 - 741   2002.5

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    J-GLOBAL

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Presentations 41

  1. 細胞診残余検体を用いた液状化検体細胞診標本の作製と、免疫組織化学的検討

    池田 勝秀, 梅宮 敏文, 佐藤 正一, 山口 良考

    国際医療福祉大学学会誌  2019.9  国際医療福祉大学学会

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  2. NK細胞のADCC活性および抗体医薬品の薬理効果に影響する遺伝子多型の解析

    大星 航, 佐藤 正一, 山口 良考, 池田 勝秀

    国際医療福祉大学学会誌  2019.9  国際医療福祉大学学会

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  3. 重症筋無力症における胸腺腫由来細胞株の樹立 治療法の開発にむけて

    山口 良考, 梅宮 敏文, 池田 勝秀, 木村 明佐子, 樋口 光徳, 鈴木 弘行

    国際医療福祉大学学会誌  2019.9  国際医療福祉大学学会

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  4. 千葉県臨床検査技師会が進める休日街頭HIV抗体検査事業について

    布施 義也, 池田 勝秀, 坂本 浩輝, 下条 小百合, 吉田 隆, 中山 茂

    千臨技会誌  2018.4  (一社)千葉県臨床検査技師会

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    Event date: 2018.4

    Language:Japanese   Presentation type:Oral presentation (general)  

    千葉県臨床検査技師会は千葉県から休日街頭HIV検査の委託事業を受けている。これまでの活動を分析したところ、HIV検査の陽性率が平日に保健所等で行われるHIV即日検査より高いという結果が得られた。また、受検者に対して行ったアンケートからは同性愛者などHIV感染ハイリスクグループへの啓発が必要であることが判明した。本事業活動はHIV感染者早期発見とHIVを主とした性感染症対策の普及啓発活動に有益であるといえる。(著者抄録)

  5. 小学生向け専門職体験イベントを通じた成田キャンパス周辺地域住民のニーズ調査 成田キャンパスに期待するもの

    牧原 由紀子, 澤 龍一, 清水 信輔, 二田水 彩, 森山 ますみ, 平野 大輔, 中村 美緒, 吉田 浩滋, 山口 良考, 池田 勝秀, 河野 弥季, 赤堀 ゆきこ, 佐藤 寛子

    国際医療福祉大学学会誌  2018.3  国際医療福祉大学学会

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    Event date: 2018.3

    Language:Japanese   Presentation type:Oral presentation (general)  

  6. 休日街頭HIV抗体検査 事業報告 受検状況

    布施 義也, 中山 茂, 吉田 隆, 下条 小百合, 池田 勝秀, 坂本 浩輝

    千臨技会誌  2018.1  (一社)千葉県臨床検査技師会

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    Event date: 2018.1

    Language:Japanese   Presentation type:Oral presentation (general)  

  7. 休日街頭HIV抗体検査 事業報告 受検者へのアンケート結果

    布施 義也, 中山 茂, 吉田 隆, 下条 小百合, 池田 勝秀, 坂本 浩輝

    千臨技会誌  2018.1  (一社)千葉県臨床検査技師会

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    Event date: 2018.1

    Language:Japanese   Presentation type:Oral presentation (general)  

  8. 腟断端に再発をきたした腹膜原発上衣腫(Ependymoma)の一例

    藤間 瑞穂, 佐々木 直志, 吉田 正行, 吉田 裕, 渡邊 麗子, 池田 勝秀, 澁木 康雄, 公平 誠, 温泉川 真由, 石川 光也, 池田 俊一, 加藤 友康, 平岡 伸介

    日本臨床細胞学会雑誌  2015.10  (公社)日本臨床細胞学会

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    Event date: 2015.10

    Language:Japanese   Presentation type:Oral presentation (general)  

  9. がん性腹膜炎の原発臓器および組織型推定に腹水セルブロック法は有用である

    隅蔵 智子, 吉田 正行, 吉田 裕, 池田 勝秀, 佐々木 直志, 温泉川 真由, 大井 隆照, 宮本 雄一郎, 岡田 智志, 石川 光也, 池田 俊一, 加藤 友康, 笠松 高弘, 九嶋 亮治

    日本婦人科腫瘍学会雑誌  2014.6  (公社)日本婦人科腫瘍学会

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    Event date: 2014.6

    Language:Japanese   Presentation type:Oral presentation (general)  

  10. 悪性中皮腫のプラクティカル・サイトロジー(形態学から報告書まで) 反応性中皮細胞の形態学と悪性中皮腫細胞との鑑別について

    磯崎 岳夫, 北村 隆司, 池田 勝秀, 岸本 浩次, 平林 寧子, 楯 玄秀, 光谷 俊幸, 太田 秀一

    日本臨床細胞学会雑誌  2009.3  (公社)日本臨床細胞学会

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    Event date: 2009.3

    Language:Japanese   Presentation type:Oral presentation (general)  

  11. ホルマリン固定パラフィン切片を用いた糸球体腎炎の免疫組織化学染色

    鈴木孝夫, 池田勝秀, 家泉佳一, 楯玄秀, 光谷俊幸, 太田善樹

    日本臨床検査技師会関東甲信学術集会  2008.8 

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    Event date: 2008.8

    Language:English   Presentation type:Oral presentation (general)  

  12. 改良型蛍光抗体法多重染色と共焦点レーザー顕微鏡を用いた抗原観察

    鈴木孝夫, 池田勝秀, 楯玄秀, 光谷俊幸

    日本臨床電子顕微鏡学会総会ならびに学術集会講演プログラム・予稿集  2004 

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    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

  13. 固定液による免疫染色の違い Invited

    池田勝秀

    第1回千葉県臨床細胞学会  2014.7 

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    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  14. 免疫染色の応用―多重染色法― Invited

    池田勝秀

    第44回組織細胞化学講習会  2019.8 

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  15. 体腔液細胞診技術—ここがポイント— Invited

    池田勝秀

    東京都細胞検査士会  2015.5 

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    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  16. 体腔液細胞診における良悪性の鑑別:免疫細胞化学的IMP3発現の検討

    池田勝秀, 楯玄秀, 鈴木孝夫, 北村隆司, 光谷俊幸

    第303回昭和医学会例会  2010.2 

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  17. 体腔液細胞診における良悪性の鑑別:免疫細胞化学的IMP3発現

    池田勝秀, 北村隆司, 鈴木孝夫, 田尻琢磨, 増永敦子, 楯玄秀, 光谷俊幸

    第48回日本臨床細胞学会秋季大会  2009.11 

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  18. 体腔液における小円形細胞腫瘍の細胞形態像

    池田勝秀, 澁木康雄, 佐々木直志, 吉田正行, 前島亜希子, 蔦幸治, 九嶋亮治, 津田均

    第53回日本臨床細胞学会総会  2012.6 

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  19. 会陰部に発生したEpithelioid sarcoma, proximal-typeの1例

    池田勝秀, 北村隆司, 増永敦子, 楯玄秀, 光谷俊幸, 土屋眞一

    第44回 日本臨床細胞学会総会  2003.6 

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  20. 人材育成ワークショップ「みんなで考えよう臨床検査のプロモーション」

    山元隆, 池田勝秀

    第66回医学検査学会  2017.6 

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  21. 乳頭状増殖を主体とした子宮体内膜癌の2例

    池田勝秀, 北村隆司, 増永敦子, 楯玄秀, 光谷俊幸, 松井成明, 佐藤育男, 塩川章

    第39回 日本臨床細胞学会秋季大会  2000.11 

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  22. アルコール固定標本を用いた免疫染色有用性

    池田勝秀, 梅宮敏文, 山口良考, 澁木康雄

    第7回国際医療福祉大学学会学術大会  2017.8 

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  23. Proximal-type epithelioid sarcoma の一例

    増永敦子, 池田勝秀, 田尻琢磨, 光谷俊幸

    第92回日本病理学会総会  2003.4 

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  24. A case of large cell neuroendocrine carcinoma of the lung

    Atsuko Masunaga, Keiichi Iezumi, Katsuhide Ikeda, Takashi Kitamura, Toshiyuki Mitsuya

    日中細胞学会  2002.11 

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  25. 細胞診残余検体を用いた液状化検体細胞診標本と、セルブロック法への応用 アルコール固定標本での基礎的検討

    池田勝秀, 梅宮敏文, 山口良考

    第8回国際医療福祉大学学会学術大会  2018.8 

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  26. 悪性中皮腫の臨床と組織診断・細胞診断 Invited

    池田勝秀

    第6回日臨技北日本支部医学検査学会  2017.10 

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  27. 免疫組織化学多重染色法 Invited

    鈴木孝夫, 池田勝秀, 柳田絵美衣

    第37回組織細胞化学講習会  2012.8 

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  28. 鼻腔原発悪性黒色腫の一例

    佐々木陽介, 北村隆司, 岸本浩次, 池田勝秀, 瀧本雅文, 光谷俊幸, 太田秀一

    第52回日本臨床細胞学会総会  2011.5 

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  29. 重症筋無力症における胸腺腫由来細胞株の樹立 治療法の開発に向けて

    山口良考, 梅宮敏文, 池田勝秀, 木村明佐子, 鈴木弘行, 樋口光徳

    第8回国際医療福祉大学学会学術大会  2018.8 

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  30. 重症筋無力症における胸腺腫由来細胞株の樹立 治療法の開発に向けて

    山口良考, 梅宮敏文, 池田勝秀, 木村明佐子, 鈴木弘行, 樋口光徳

    第7回国際医療福祉大学学会学術大会  2017.8 

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  31. 術後1年3ヶ月で再発を認めた喉頭非定型カルチノイドの1例

    神保容子, 北村隆司, 池田勝秀, 増永敦子, 楯玄秀, 光谷俊幸

    第21回 日本臨床細胞学会神奈川地方会  2002.9 

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  32. 腹水細胞診で認めた異型細胞の判定に苦慮した卵巣胚細胞腫瘍2例の検討

    千木良浩史, 澁木康雄, 池田勝秀

    第30回関東臨床細胞学会  2016.9 

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  33. 胞巣型横紋筋肉腫の一例

    池田勝秀, 北村隆司, 田尻琢磨, 増永敦子, 楯玄秀, 光谷俊幸

    第49回 日本臨床細胞学会総会  2008.6 

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  34. 細胞浮遊液を用いた免疫グロブリン軽鎖の検出に関する検討

    鈴木孝夫, 岸本浩次, 家泉佳一, 池田勝秀, 北村隆司, 増永敦子, 楯玄秀, 光谷俊幸

    第42回日本臨床細胞学会総会  2001.6 

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  35. 用手法による免疫組織化学染色のtips

    鈴木孝夫, 岸本浩次, 平山淑子, 池田勝秀, 楯玄秀, 光谷俊幸

    第18回日本臨床検査技師会関東甲信学術集会  2009.11 

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  36. 特異な細胞像を呈した腹膜原発癌の一例

    池田勝秀, 北村隆司, 田尻琢磨, 増永敦子, 楯玄秀, 光谷俊幸

    第21回 日本臨床細胞学会関東連合会学術集会  2007.9 

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  37. 悪性中皮腫の細胞診断

    磯崎岳夫, 北村隆司, 池田勝秀, 濱川真治, 津田祥子, 平林寧子, 光谷俊幸, 太田秀一

    東京都医学検査学会  2010.3 

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  38. 子宮頚部Glassy cell carcinomaの1例

    池田勝秀, 北村隆司, 家泉佳一, 岸本浩次, 鈴木孝夫, 田尻琢磨, 増永敦子, 楯玄秀, 光谷俊幸

    第19回 日本臨床細胞学会神奈川県支部地方部会・学術集会  2001.3 

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  39. 女性付属器発生 癌肉腫8例の体腔液細胞像

    池田勝秀, 北村隆司, 鈴木孝夫, 増永敦子, 楯玄秀, 光谷俊幸, 土屋眞一

    第42回日本臨床細胞学会秋季大会  2003.11 

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  40. 大腸癌培養細胞株を使ったマウス移植腫瘍形成プログラムの確立

    梅宮敏文, 池田勝秀, 山口良考, 北川元生

    第8回国際医療福祉大学学会学術大会  2018.8 

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  41. 反応性中皮細胞の形態学と悪性中皮腫細胞との鑑別について

    磯崎岳夫, 北村隆司, 池田勝秀, 岸本浩次, 平林寧子, 楯玄秀, 光谷俊幸, 太田秀一

    第50回 日本臨床細胞学会総会  2009.6 

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KAKENHI (Grants-in-Aid for Scientific Research) 1

  1. 体腔液細胞診におけるAI診断の開発-細胞像変化への対応と標本作製技術の検討-

    Grant number:21K18077  2021.4 - 2025.3

    日本学術振興会  科学研究費助成事業  若手研究

    池田勝秀

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

 

Teaching Experience (On-campus) 31

  1. データサイエンス概論Ⅰ

    2021

  2. Pathology

    2021

  3. Anatomy II

    2021

  4. Anatomy I

    2021

  5. 基礎セミナーA

    2021

  6. 生物学実験

    2021

  7. 人体構造学実習

    2021

  8. 医療英語ⅡB

    2021

  9. 医療英語ⅠB

    2021

  10. 生物学基礎Ⅱ

    2021

  11. 臨床病理学演習

    2021

  12. Laboratory Pathology Practice D

    2021

  13. Anatomy and Physiology IB

    2021

  14. Laboratory Pathology and Cytology Practice A

    2021

  15. General Pathology

    2021

  16. 解剖学Ⅱ

    2020

  17. 基礎セミナーA

    2020

  18. 医療英語ⅠB

    2020

  19. 臨床病理学演習

    2020

  20. 生物学基礎Ⅱ

    2020

  21. 生物学実験

    2020

  22. 検査技術科学特論Ⅰ

    2020

  23. Laboratory Pathology and Cytology Practice A

    2020

  24. Laboratory Pathology and Cytology I

    2020

  25. Anatomy Practice

    2020

  26. Pathology B

    2020

  27. General Pathology

    2020

  28. PathologyA

    2020

  29. Laboratory Pathology and Cytology II

    2020

  30. Laboratory Pathology Practice D

    2020

  31. Anatomy and Physiology IB

    2020

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Teaching Experience (Off-campus) 6

  1. 病理学

    2020.4 名古屋大学医学部保健学科)

  2. 組織学、組織学実習

    2020.4 名古屋大学医学部保健学科)

  3. 細胞診断検査学、細胞診断検査学実習

    2020.4 名古屋大学医学部保健学科)

  4. 病理検査学、病理検査学実習

    2020.4 名古屋大学医学部保健学科)

  5. 病理検査学、実習

    2016.4 - 2020.3 国際医療福祉大学成田保健医療学部)

  6. 細胞診断検査学

    2016.4 - 2020.3 国際医療福祉大学成田保健医療学部)

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