Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1159/000526131

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1159/000526098

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

DOI： 10.1016/j.jbc.2022.101833

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Phosphatidylinositol 3-kinase (PI3K) signaling promotes the differentiation and proliferation of regulatory B (Breg) cells, and the lipid phosphatase phosphatase and tensin homolog deleted on chromosome 10 (PTEN) antagonizes the PI3K-Akt signaling pathway. We previously demonstrated that cardiac Akt activity is increased and that restraint stress exacerbates hypertension and both heart and adipose tissue (AT) inflammation in DS/obese rats, an animal model of metabolic syndrome (MetS). We here examined the effects of restraint stress and pharmacological inhibition of PTEN on heart and AT pathology in such rats. Nine-week-old animals were treated with the PTEN inhibitor bisperoxovanadium-pic [bpV(pic)] or vehicle in the absence or presence of restraint stress for 4 weeks. BpV(pic) treatment had no effect on body weight or fat mass but attenuated hypertension in DS/obese rats subjected to restraint stress. BpV(pic) ameliorated left ventricular (LV) inflammation, fibrosis, and diastolic dysfunction as well as AT inflammation in the stressed rats. Restraint stress reduced myocardial capillary density, and this effect was prevented by bpV(pic). In addition, bpV(pic) increased the proportions of Breg and B-1 cells as well as reduced those of CD8+ T and B-2 cells in AT of stressed rats. Our results indicate that inhibition of PTEN by bpV(pic) alleviated heart and AT inflammation in stressed rats with MetS. These positive effects of bpV(pic) are likely due, at least in part, to a reduction in blood pressure, an increase in myocardial capillary formation, and an altered distribution of immune cells in fat tissue that result from the activation of PI3K-Akt signaling.

DOI： 10.14814/phy2.15165

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Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.14814/phy2.15165

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：S. Karger AG  

<b><i>Introduction:</i></b> Liquid-based cytology (LBC) is increasingly used for nongynecologic applications. However, the cytological preparation of LBC specimens is influenced by the processing technique and the preservative used. In this study, the influence of the processing techniques and preservatives on cell morphology was examined mathematically and statistically. <b><i>Methods:</i></b> Cytological specimens were prepared using the ThinPrep (TP), SurePath (SP), and AutoSmear methods, with 5 different preservative solutions. The cytoplasmic and nuclear areas of Papanicolaou-stained specimens were measured for all samples. <b><i>Results:</i></b> The cytoplasmic and nuclear areas were smaller in cells prepared using the 2 LBC methods, compared to that prepared using the AutoSmear method, irrespective of the preservative used. The cytoplasmic and nuclear areas of cells prepared using the SP method were smaller than those of cells prepared using the TP method, irrespective of the preservative used. There were fewer differences among the cytoplasmic areas of cells prepared with different preservative solutions using the TP method; however, the cytoplasmic areas of cells prepared using the SP method changed with the preservative solution used. <b><i>Conclusions:</i></b> The most significant difference affecting the cytoplasmic and nuclear areas was the processing technique. The TP method increased the cytoplasmic and nuclear areas, while the methanol-based PreservCyt solution enabled the highest enlargement of the cell. LBC is a superior preparation technique for standardization of the specimens. Our results offer a better understanding of methods suitable for specimen preparation for developing precision AI-based diagnosis in cytology.

DOI： 10.1159/000519335

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DOI： 10.1152/ajpheart.00657.2020

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DOI： 10.1111/nyas.14546

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DOI： 10.3892/ol.2020.12073

Language：English   Publishing type：Research paper (scientific journal)  

OBJECTIVES: Evidence suggests that visceral adipose tissue (VAT) and subcutaneous adipose tissue (SAT) should be considered as distinct types of white fat. Although VAT plays a key role in metabolic syndrome (MetS), the role of subcutaneous adipose tissue (SAT) has been unclear. DahlS.Z-Leprfa/Leprfa (DS/obese) rats, an animal model of MetS, develop adipocyte hypertrophy and inflammation to similar extents in SAT and VAT. We have now investigated the effects of salt loading and SAT removal on cardiac, renal, and VAT pathology in DS/obese rats. METHODS: DS/obese rats were subjected to surgical removal of inguinal SAT or sham surgery at 8 weeks of age. They were provided with a 0.3% NaCl solution as drinking water or water alone for 4 weeks from 9 weeks of age. RESULTS: Salt loading exacerbated hypertension, insulin resistance, as well as left ventricular (LV) hypertrophy, inflammation, fibrosis, and diastolic dysfunction in DS/obese rats. It also reduced both SAT and VAT mass but aggravated inflammation only in VAT. Although SAT removal did not affect LV hypertrophy in salt-loaded DS/obese rats, it attenuated hypertension, insulin resistance, and LV injury as well as restored fat mass and alleviated inflammation and the downregulation of adiponectin gene expression in VAT. In addition, whereas salt loading worsened renal injury as well as upregulated the expression of renin-angiotensin-aldosterone system-related genes in the kidney, these effects were suppressed by removal of SAT. CONCLUSIONS: SAT removal attenuated salt-induced exacerbation of MetS and LV and renal pathology in DS/obese rats. These beneficial effects of SAT removal are likely attributable, at least in part, to inhibition of both VAT and systemic inflammation.

DOI： 10.1038/s41387-020-00132-1

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1159/000503268

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DOI： 10.1159/000500769

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DOI： http://doi.org/10.1038/s41387-020-00132-1

Authorship：Last author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：The Society of Analytical Bio-Science  

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Background: Small round cell tumors (SRCTs) are a group of tumors composed of small, round, and uniform cells with high nuclear/cytoplasmic (N/C) ratios. The appearance of SRCT neoplastic cells in the effusion fluid is very rare. We reported the cytomorphological findings of SRCTs in effusion cytology, and performed statistical and mathematical analyses for a purpose to distinguish SRCTs.
Materials and Methods: We analyzed the cytologic findings of effusion samples from 40 SRCT cases and measured the lengths of the nuclei, cytoplasms, and the cell cluster areas. The SRCT cases included 14 Ewing sarcoma (EWS)/primitive neuroectodermal tumor cases, 5 synovial sarcoma cases, 6 rhabdomyosarcoma cases, 9 small cell lung carcinoma (SCLC) cases, and 6 diffuse large B-cell lymphoma (DLBL) cases.
Results: Morphologically, there were no significant differences in the nuclear and cytoplasmic lengths in cases of EWS, synovial sarcoma, and rhabdomyosarcoma. The cytoplasmic lengths in cases of SCLC and DLBL were smaller than those of EWS, synovial sarcoma, and rhabdomyosarcoma. The nuclear density of the cluster in SCLC was higher than that in other SRCTs, and cases of DLBL showed a lack of anisokaryosis and anisocytosis.
Conclusion: We believe that it might be possible to diagnose DLBL and SCLC from cytologic analysis of effusion samples but it is very difficult to use this method to distinguish EWS, synovial sarcoma, and rhabdomyosarcoma. Statistical and mathematical analyses indicated that nuclear density and dispersion of nuclear and cytoplasmic sizes are useful adjuncts to conventional cytologic diagnostic criteria, which are acquired from experience.

DOI： 10.4103/0970-9371.182529

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：WILEY-BLACKWELL  

AIMS: Lobular neoplasias (LNs) are typically small, clinically undetectable breast lesions, but some LNs are of clinical significance. The aim of this study was to clarify the histopathological characteristics of clinically overt (symptomatic) LNs and early invasive LNs. METHODS AND RESULTS: Sixty-two surgically resected LNs, including eight with early invasion (≤10 mm), were classified into the following groups: (i) symptomatic and occult; and (ii) early invasive and non-invasive. Six histopathological factors, including the Ki67 labelling index (LI), were assessed and analysed by logistic regression models. On multivariate analysis, tumour size (P = 0.008), mitotic counts (P = 0.006) and Ki67 LI (P = 0.035) were risk factors for symptomatic features, and tumour size (P = 0.009) and Ki67 LI (P = 0.015) were risk factors for early invasive lesions. In the eight LNs with invasion, the symptomatic and occult subgroups showed differing nuclear atypia and structural patterns, but both lesions extended widely (22-96 mm). CONCLUSIONS: Wide extension and higher proliferation activity were characteristic features of symptomatic LNs and LNs with early invasion.

DOI： 10.1111/his.12356

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Language：English   Publishing type：Research paper (scientific journal)   Publisher：SPANDIDOS PUBL LTD  

The Working Group of the Japanese Society of Clinical Cytology was assembled to assess the current status of breast cytology in Japan by conducting a large-scale survey regarding the accuracy of fine-needle aspiration biopsy (FNAB) in Japan. We collected data and investigated the status of breast cytological diagnosis at 12 different cooperating facilities in Japan, and re-evaluated their false-negative and false-positive cases. Among 30,535 individuals who underwent a breast cytological examination, analyses were conducted on 10,890 individuals (35.7%) in whom cytological diagnoses were confirmed by histology. Among these patients, the cytological diagnosis had an inadequate rate of 17.7%, an indeterminate rate of 7.8%, a positive predictive value of 'malignancy suspected' cells of 92.4%, an absolute sensitivity of 76.7%, a complete sensitivity of 96.7%, a specificity of 84.3%, a positive predictive value of 'malignant' cells of 99.5%, a false-negative value of 3.31%, a false-positive value of 0.25% and an accuracy rate of 88.0%. Subsequently, 297 false-negative and 23 false-positive cases were re-evaluated and several factors were characterized (i.e. histological type, tumor size and misread points). This survey collected data from a large number of cases for breast FNAB. Based on our survey, the accuracy of FNAB in Japan was relatively high compared with the goal of assessment of diagnostic accuracy. However, there were some false-negative and false-positive cases. Improvements in accuracy resulting from the learning points in the present study will lead to more useful and reliable diagnostic tools in clinical practice.

DOI： 10.3892/or.2012.2014

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Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：AMER SOC CLINICAL PATHOLOGY  

The most commonly used fixative in effusion cytology is formalin. In the present study, the immunocytochemical properties of formalin-fixed and alcohol-fixed specimens were compared to evaluate the usefulness of alcohol-fixed cytocentrifuged preparations for routine cytologic diagnosis. A total of 269 effusion samples and 17 primary antibodies were used. The sensitivity of immunocytochemical studies in alcohol-fixed specimens was similar and correlated to that of formalin-fixed specimens, suggesting that alcohol-fixed cytocentrifuged preparations are useful in effusion cytology. Pretreatment with or without heat-induced antigen retrieval revealed that antigen retrieval was unnecessary for immunocytochemical studies with most primary antibodies in alcohol-fixed cytocentrifuged preparations. The present study describes the use of immunocytochemical studies with alcohol-fixed cytocentrifuged preparations for diagnosis in routine effusion cytology.

DOI： 10.1309/AJCPAH5WR2LFTUSI

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To differentiate reactive mesothelial cells (RMs) from metastatic carcinoma and malignant mesothelioma (MM) in effusion cytology is crucial for the cytologic diagnosis and the management of the patients. In the present study, the immunocytochemical staining profile of the epithelial membrane antigen (EMA), the insulin-like growth factor-II mRNA-binding protein 3 (IMP3), and the glucose transporter-1 (GLUT-1) was examined to distinguish RMs from malignant cells. A total of 171 pleural (n = 87) and peritoneal (n = 84) effusion specimens, including 50 benign effusions with RMs, 11 MM effusions, and 110 metastatic malignant effusions, were evaluated for immunocytochemistry. EMA, IMP3, monoclonal GLUT-1, and polyclonal GLUT-1 immunoreactivity were observed in 26.0%, 6.0%, 20.0%, and 18.0% of RMs, respectively. In contrast to RMs, the immunoreactivity in MM was 100%, 36.4%, 100%, and 90.9%; adenocarcinoma (AC) was 100%, 80.8%, 81.7%, and 72.1%; squamous-cell carcinoma was 83.3%, 83.3%, 83.3%, and 66.7%. EMA, IMP3, mGLUT-1, and pGLUT-1 expressions were observed in 98.4%, 65.6%, 88.5%, and 75.4% in the pleural effusion with malignant cells, and 100%, 88.3%, 78.3%, and 71.7% in ascites containing malignant cells, respectively. The findings of the present study indicate that the immunocytochemical staining for EMA, IMP3, and GLUT-1 is a useful diagnostic tool for distinguishing effusions containing malignant cells from those that contain benign cells, and in particular, we suggest that the combination of mGLUT-1 and EMA, and IMP3 and EMA are extremely useful in pleural effusion and in ascites, respectively.

DOI： 10.1002/dc.21398

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DOI： 10.1016/j.acthis.2009.08.007

Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

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Differentiating reactive mesothelial cells from metastatic carcinoma and malignant mesothelioma is critical in effusion cytology. Numerous immunohistochemical/cytochemical reports use various antibodies in effusion samples, and most antibodies differentiate metastatic adenocarcinoma from malignant mesothelioma, but no antibodies help distinguish malignant mesothelioma from reactive mesothelial cells. A mouse monoclonal antibody (IMP3/L523S) against KOC is a 580-amino acid oncofetal RNA-binding protein containing 4 K homology domains. IMP3/L523S has been identified in several human malignant tumors. The immunocytochemical staining profile of IMP3 was determined in 95% alcohol-fixed cytologic effusion specimens. A total of 229 cases of pleural and peritoneal effusion cytospecimens were evaluated for the study, including 39 benign effusions with reactive mesothelial cells and 190 metastatic malignant effusions. IMP3 immunoreactivity was observed in 2 (5.1%) of 39 cases of reactive mesothelial cells, 138 (72.6%) of 190 cases of malignant effusion, 4 (36.4%) of 11 cases of malignant mesothelioma, 106 (75.7%) of 140 cases of metastatic adenocarcinoma, and 8 (100%) of 8 cases of squamous cell carcinoma. The overall specificity for the diagnosis of malignancy was 94.9%, whereas the sensitivity was 72.6%. In the peritoneal effusions, the sensitivity for the diagnosis of metastatic adenocarcinoma to distinguish reactive mesothelial cells was 92.3%. In conclusion, IMP3 staining is present in many carcinomas and is not a useful marker for distinguishing between carcinomas arising in different organs. However, the IMP3 antibody is a highly specific marker for malignant lesions, and thus, IMP3 staining is useful for distinguishing neoplastic cells from reactive mesothelial cells in effusion samples.

DOI： 10.1016/j.humpath.2009.04.030

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Effusion cytological analyses of amelanotic malignant melanoma (AMM) are very rare and no concise description of AMM related cytomorphologic features using effusion have been reported. Here, we report the cytomorphological, immunohistochemical, and immunocytochemical findings in the effusion cytology of six cases of malignant melanoma (MM), one case of AMM, and five cases of melanotic malignant melanoma. Papanicolaou-stained smears exhibited conspicuous nucleoli, multinucleation, and cytoplasmic vacuolization in all of the MM cases. In addition, the AMM case displayed numerous mitotic figures and intranuclear cytoplasmic inclusions. With regard to the immunohistochemistry findings, all six cases of melanoma were positive for Melan-A/MART-1, HBME-1, and S-100. In the immunohistochemistry analyses, five of six cases of melanoma were positive for WT-1, as was the AMM specimen. Furthermore, because the effusion analysis of malignant mesothelioma proved positive for WT-1, it should be noted that WT-1 effusion analysis is not an appropriate means to distinguish between MM and malignant mesothelioma. We suggest that it is important to recognize cytomorphologic characteristics, such as melanin pigment, conspicuous nucleoli, multinucleation, and cytoplasmic vacuolization, and to choose appropriate antibodies for the correct diagnosis of MM in effusion.

DOI： 10.1002/dc.21054

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Well-differentiated papillary mesothelioma (WDPM), a distinct subtype of diffuse malignant mesothelioma, usually occurs in the peritoneum and is seen most commonly in women of reproductive age. Histologic features of WDPM include papillary growth and stout fibrous cores surrounded by a single layer of tumor cells. We present the case of a 73-year-old woman without subjective symptoms who showed signs of peritoneal effusion during a routine examination and for whom cytologic examination of the ascitic fluid was performed. Many spherical clusters, with a smooth external surface composed of a single layer of uniform cuboidal cells, were observed. Within each cluster, a collagenous ball showed light green Papanicolaou staining. Immunohistochemistry of surgical specimens showed tumor cells positive for calretinin, D(2)-40, and HBME-1 staining. The histologic diagnosis was WDPM. The identification of a collagenous ball within these clusters is a useful cytologic finding for the diagnosis of WDPM. WDPM should be suspected when numerous collagenous balls are present by effusion cytology and isolated cells are not.

DOI： 10.1002/dc.20824

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OBJECTIVE: Several Cytokeratin (CK) isoforms have been analyzed in cervical intraepithelial lesions. However, previously reported numbers of specimens have been too low to evaluate any correlation between CK and CIN. METHODS: We examined the immunohistochemical staining of p16, CK8, and CK17 in 134 cervical tissues obtained by punch biopsy and graded as follows: CIN I (n=39), CIN II (n=31), CIN III (n=43), SCC (n=21). RESULTS: p16 staining was identified in 74.4% of CIN I, 93.6% of CIN II, 97.7% of CIN III, and 100% of SCC cases. CK8 and CK17 staining were identified in 12.8% and 33.3% of CIN I, 22.6% and 58.1% of CIN II, 62.8% and 81.4% of CIN III, and 71.4% and 95.2% of SCC cases, respectively. Interestingly, positivity for CK8 and CK17 correlated with increasing lesion grade of the intraepithelial lesions and also correlated with p16 staining (p16, p=0.0008; CK8, p<0.0001, and CK17, p<0.0001), and a coordinate expression profile of CK8[+]/CK17[+] correlates with increasing CIN grade and carcinoma (likewise, a coordinate expression profile of CK8[-]/CK17[-] correlates with decreasing CIN grade and absence of carcinoma), but expression of just CK8 (CK8[+]/CK17[-]) or just CK17 (CK8[-]/CK17[+]) does not correlate with increasing CIN grade and carcinoma. CONCLUSIONS: Results of the present study showed that p16, CK8, and CK17 immunostaining differed according to the degree of cervical intraepithelial lesions and SCC, and surprisingly, that staining was significantly correlated with increasing lesion grade of CIN and SCC.

DOI： 10.1016/j.ygyno.2007.11.042

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We report a case of a clear cell adenocarcinoma arising from a giant cystic adenomyosis, with immunohistochemical analysis of p53 and laminin-5 gamma2 chain overexpression. Microscopically, not only clear cell adenocarcinoma showing myometrial invasion but also single-layered clear cell adenocarcinoma cells lining the cyst wall were observed. Transition from these single-layered tumor cells to papillary proliferative lesions of various degrees was recognized. Moreover, these tumor cells were continuous with minimal atypical cells. Although the tumor cells within the uterus showed a low positive cell ratio for p53, the metastatic foci showed a remarkable p53 overexpression. Laminin-5 gamma2 chain expression was low in papillary proliferation and high in myometrial invasion and metastatic foci. The single-layered tumor cells showing non-invasive proliferation also contained laminin-5 gamma2 chain-positive cells. When non-invasive tumor cells were considered to be at an early stage in tumor progression, some tumor cells had already acquired an invasive feature. p53 overexpression was not related to expression of the laminin-5 gamma2 chain.

DOI： 10.1016/j.prp.2008.02.005

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DOI： 10.1111/j.1365-2559.2007.02742.x

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We describe the fine-needle aspiration cytology features of a primary parathyroid cancer and of the local recurrent and distant metastatic lesions. The presence of prognostic factors Ki-67 and proliferating cell nuclear antigen (PCNA) was compared immunohistochemically between primary parathyroid carcinoma and related metastatic and recurrent foci. Flow cytometric DNA analysis was also performed to investigate any chromosomal abnormality of the parathyroid carcinoma. Cytologic examination of the endocrine tumor showed that it comprised a loose cohesive cluster and tumor cells with granular cytoplasm and mild nuclear atypia, but for purposes of cytodiagnosis, it is difficult to determine whether such a neoplasm is malignant on the basis of morphology alone. Immunohistochemical analysis showed that Ki-67 and PCNA labeling indices were higher in the recurrent and metastasized carcinomas than in the primary cancer, suggesting that neoplastic cells become more malignant in the recurrent and metastasized foci. To our knowledge, this is the first report describing not only cytopathologic but also immunocytologic differences between primary parathyroid cancer and the metastatic lesion.

DOI： 10.1002/dc.20399

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DOI： 10.18926/AMO/31955

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We describe unusual cytologic features of the peritoneal fluid in a patient with immature ovarian teratoma. Immature embryonal and neuroectodermal components of such tumors are rarely observed in the ascites; to our knowledge, there have been only three reports of cytologic findings of immature teratoma cells in the ascites. Our patient was a 26-yr-old woman who presented with a huge pelvic mass. A grade 3 immature right ovarian teratoma was diagnosed pathologically. Cytologic examination of the ascitic fluid revealed a variety of neoplastic cells, including immature neuroepithelial cells forming rosette-like structures, keratinized squamous cells, squamoid metaplastic cells, and immature glial-appearing cells. Immunohistochemical analysis revealed that immature gastrointestinal elements and yolk sac elements were positive for alpha-fetoprotein. This is the first description of the cytologic features of non-neuroepithelial elements in the ascitic fluid in a patient with immature ovarian teratoma.

DOI： 10.1002/dc.20259

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OBJECTIVE: Expression of Matrix metalloproteinase 7 (MMP-7) and Ki-67 by carcinoma components (CCs) and sarcoma components (SCs) in carcinosarcoma of the female reproductive organs has been investigated by conventional methods, but analysis with immunohistochemical staining of multiple antigens has not been reported. We report the profiles of expression of MMP-7 and Ki-67 in carcinosarcoma determined with immunohistochemical staining techniques. METHODS: We used antibodies against epithelial antigen (EA), epithelial membrane antigen (EMA), and vimentin for immunofluorescence double staining of ascitic fluid in eight cases of carcinosarcoma of female reproductive organs. We also used immunohistochemical triple staining to compare MMP-7 and Ki-67 expression between CCs and SCs in the primary site of carcinosarcoma. RESULTS: Immunofluorescence analysis revealed that all neoplastic cells in the ascitic fluid were positive for EA or EMA, indicating that these cells were CCs. Immunohistochemical analyses of the primary organ of carcinosarcoma revealed that MMP-7 was expressed on CCs in four of eight cases of carcinosarcoma, whereas MMP-7 was not expressed on SCs. The average Ki-67 labeling index (LI) in CCs and SCs was 51.8% and 28.6%, respectively. The difference in Ki-67 LI between CCs and SCs was statistically significant (t test for paired samples, P = 0.0173). CONCLUSIONS: This is the first study to examine carcinosarcoma of the female reproductive organ by immunohistochemical staining for multiple antigens, which allows analysis of mixed tumor elements. In addition, we found that expression of MMP-7 and the average Ki-67 LI differ between CCs and SCs in carcinosarcoma. The predominance of CCs as the malignant cells in the ascitic fluid may be due to cytological differences between CCs and SCs of carcinosarcoma.

DOI： 10.1016/j.ygyno.2004.12.024

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DOI： 10.1159/000326155

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DOI： 10.1111/j.1440-1827.2004.01671.x

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Language：Japanese  

Authorship：Lead author,　Corresponding author   Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

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Language：Japanese   Publisher：Japanese Society of Clinical Cytology  

Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Clinical Cytology  

CiNii Research

Language：Japanese   Publisher：The Japanese Society of Clinical Cytology  

<B><I>Purpose</I>:</B> Cytologic features of intraductal papilloma (IDP) and differential diagnosis between IDP and well-differentiated breast carcinoma.<BR><I>Diagnosis</I>: Typical IDP shows papillary and cohesive clusters composed of duct epithelium, myoepithelium, and stroma, and discohesive cells are rare in the background. In IDP with necrosis, necrotic debris is massive and epithelial cells show degenerated nuclei, but there are no differences in the N/C ratio between IDP cells and normal columnar epithelia.<BR><B><I>Differential Diagnosis</I>:</B> To differentiate IDP from papillary carcinoma, it is important to observe 1) cohesiveness among epithelial cells and between epithelial cells and stromal tissues; 2) presence or absence of myoepithelial cells in clusters ; and 3) cytologic atypia of discohesive clusters (in particular, the features of chromatin and N/C ratio). Important differential diagnostic considerations are to distinguish cohesive clusters of IDP from those of well-differentiated breast cancer, it is important to pay attentions to 1) cellular polarity toward the lumen, 2) cohesiveness of clusters, and 3) presence or absence of metaplastic changes.<BR><B><I>Conclusion</I>:</B> Because cytological features of IDP are quite similar to those of well-differentiated breast carcinoma, it is important for correct diagnosis to keep in mind and image cytological characteristics of IDP and cytologic differences from welldifferentiated breast carcinoma.

DOI： 10.5795/jjscc.42.149

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CiNii Research

Total pages：ix, 183p   Language：Japanese

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Language：Japanese   Publisher：神奈川県臨床細胞学会  

背景:濾胞性リンパ腫は、リンパ節に優位に浸潤し多発性リンパ節腫大を生じるが、ときに節外部位に浸潤する場合もある。今回われわれは、耳下腺浸潤が認められた濾胞性リンパ腫の1例を経験したので報告する。症例:61歳女性。左耳下腺内に辺縁不整な30mm大の腫瘤と多発性の頸部リンパ節腫大を認め、耳下腺癌、悪性リンパ腫、vichrow転移が疑われた。耳下腺腫瘤の超音波ガイド下穿刺吸引細胞診では、少数の上皮細胞を背景に核にくびれを有する小型〜中型の異型リンパ球がみられ、Class III"悪性リンパ腫の疑い"と判定された。耳下腺腫瘍摘出術、左頸部リンパ節生検が行われ、濾胞性リンパ腫grade1およびリンパ腫細胞の耳下腺浸潤と診断された。結論:本症例は出現した異型リンパ球の形態、および単調な細胞像により、唾液腺穿刺吸引細胞診にてリンパ球が多数採取される良性病変や、悪性リンパ腫を除く悪性腫瘍性病変との鑑別が可能であった。唾液腺悪性リンパ腫は、原発のほかに近接するリンパ節から浸潤する場合があることから臨床所見を考慮し判定することが重要と考えられた。(著者抄録)

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DOI： 10.11477/mf.1543101265

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DOI： 10.11477/mf.1543100105

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Language：Japanese   Publisher：The Japanese Society of Clinical Cytology  

<B><I>Purpose</I>:</B> Cytologic features of intraductal papilloma (IDP) and differential diagnosis between IDP and well-differentiated breast carcinoma.<BR><I>Diagnosis</I>: Typical IDP shows papillary and cohesive clusters composed of duct epithelium, myoepithelium, and stroma, and discohesive cells are rare in the background. In IDP with necrosis, necrotic debris is massive and epithelial cells show degenerated nuclei, but there are no differences in the N/C ratio between IDP cells and normal columnar epithelia.<BR><B><I>Differential Diagnosis</I>:</B> To differentiate IDP from papillary carcinoma, it is important to observe 1) cohesiveness among epithelial cells and between epithelial cells and stromal tissues; 2) presence or absence of myoepithelial cells in clusters ; and 3) cytologic atypia of discohesive clusters (in particular, the features of chromatin and N/C ratio). Important differential diagnostic considerations are to distinguish cohesive clusters of IDP from those of well-differentiated breast cancer, it is important to pay attentions to 1) cellular polarity toward the lumen, 2) cohesiveness of clusters, and 3) presence or absence of metaplastic changes.<BR><B><I>Conclusion</I>:</B> Because cytological features of IDP are quite similar to those of well-differentiated breast carcinoma, it is important for correct diagnosis to keep in mind and image cytological characteristics of IDP and cytologic differences from welldifferentiated breast carcinoma.

DOI： 10.5795/jjscc.42.149

CiNii Research

J-GLOBAL

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Language：Japanese  

J-GLOBAL

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Language：Japanese  

J-GLOBAL

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Language：Japanese   Publisher：特定非営利活動法人日本臨床細胞学会  

CiNii Books

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Event date： 2019.9

Language：Japanese   Presentation type：Oral presentation (general)  

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Event date： 2019.9

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Event date： 2018.4

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千葉県臨床検査技師会は千葉県から休日街頭HIV検査の委託事業を受けている。これまでの活動を分析したところ、HIV検査の陽性率が平日に保健所等で行われるHIV即日検査より高いという結果が得られた。また、受検者に対して行ったアンケートからは同性愛者などHIV感染ハイリスクグループへの啓発が必要であることが判明した。本事業活動はHIV感染者早期発見とHIVを主とした性感染症対策の普及啓発活動に有益であるといえる。(著者抄録)

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