Updated on 2024/10/03

写真a

 
MIYAKE Yasuyuki
 
Organization
Graduate School of Medicine Designated associate professor
Title
Designated associate professor
Contact information
メールアドレス
External link

Degree 3

  1. Ph.D. （ 2006.3   Osaka University ） 

  2. Master(Integrated Biosciences) （ 2002.3   The University of Tokyo ） 

  3. BSc （ 2000.3   Shinshu University ） 

To the head of Degree.▲

Research Interests 12

  1. RNA virus

  2. Influenzavirus

  3. vRNPs

  4. ubiquitin

  5. HDAC6

  6. molecular structure

  7. molecular structure

  8. Ubiquitin

  9. Histone Deacetylase

  10. influenza virus

  11. RNA virus

  12. molecular function

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Research Areas 4

  1. Life Science / Molecular biology  / Molecular Biology

  2. Life Science / Virology  / Virology

  3. Life Science / Cell biology

  4. Life Science / Structural biochemistry

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Current Research Project and SDGs 1

  1. ウイルス感染における宿主因子の動態と分子機能の解明

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Research History 9

  1. Nagoya University   Department of Virology   Designated associate professor

    2023.4

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    Country：Japan

  2. Nagoya University   Graduate School of Medicine   Assistant Professor

    2020.4 - 2023.3

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    Country：Japan

  3. University of Bristol   School of Cellular and Molecular Medicine   Researcher

    2018.5 - 2020.3

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    Country：United Kingdom

  4. University of Bristol   Faculty of Life Sciences, School of Cellular and Molecular Medicine   Visiting Scientist

    2018.5 - 2020.3

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    Country：United Kingdom

  5. Nagoya University   Graduate School of Medicine   JSPS Research Fellow

    2018.4 - 2020.3

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    Country：Japan

  6. Shinshu University   Researcher

    2016.4 - 2018.3

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    Country：Japan

  7. Friedrich Miescher Institute for Biomedical Research   Epigenetics group   Researcher

    2010.3 - 2015.12

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    Country：Switzerland

  8. Kyoto University   Graduate School of Biostudies   Researcher

    2006.4 - 2010.3

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    Country：Japan

  9. RIKEN   Laboratory of Cellular Physiology   Student Trainee

    2002.4 - 2006.3

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    Country：Japan

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Education 3

  1. Osaka University   Graduate School, Division of Medical Sciences

    - 2006.3

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    Country： Japan

  2. The University of Tokyo

    - 2002.3

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    Country： Japan

  3. Shinshu University   Faculty of Science   Biological Science

    - 2000.3

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    Country： Japan

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Professional Memberships 6

  1. 日本分子生物学会

    2000.12

  2. 日本ウイルス学会

    2020.9

  3. 日本細胞生物学会

    2022.4

  4. 日本細胞生物学会

    2022.3

  5. 日本ウイルス学会

    2020.10

  6. THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

    2002

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Awards 2

  1. Pfizer Forschungspreis (Infektiologie, Rheumatologie und Immunologie)

    2016.1   Pfizer  

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    Award type：International academic award (Japan or overseas)  Country：Switzerland

  2. CMM Awayday Best Poster Prize

    2019.1   University of Bristol   Influenza virus uses transportin 1 for vRNP debundling during cell entry

    Miyake Yasuyuki

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Papers 10

  1. Influenza virus uses transportin 1 for vRNP debundling during cell entry Reviewed International coauthorship

    Miyake Y, Keusch JJ, Decamps L, Ho-Xuan H, Iketani S, Gut H, Kutay U, Helenius A, Yamauchi Y

    Nature Microbiology   Vol. 4 ( 4 ) page： 578 - 586   2019.4

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/s41564-018-0332-2

  2. Structural insights into HDAC6 tubulin deacetylation and its selective inhibition Reviewed

    Miyake Y, Keusch JJ, Wang L, Saito M, Hess D, Wang X, Melancon BJ, Helquist P, Gut H, Matthias P

    Nature Chemical Biology   Vol. 12 ( 9 ) page： 748 - 754   2016.9

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/nchembio.2140

  3. Influenza A virus uses the aggresome processing machinery for host cell entry Reviewed

    Banerjee I, Miyake Y, Nobs SP, Schneider C, Horvath P, Kopf M, Matthias P, Helenius A, Yamauchi Y

    Science   Vol. 346 ( 6208 ) page： 473 - 477   2014.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1126/science.1257037

  4. RPA-like mammalian Ctc1-Stn1-Ten1 complex binds to single-stranded DNA and protects telomeres independently of the Pot1 pathway Reviewed

    Miyake Y, Nakamura M, Nabetani A, Shimamura S, Tamura M, Yonehara S, Saito M, Ishikawa F

    Molecular cell   Vol. 36 ( 2 ) page： 193 - 206   2009.10

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1016/j.molcel.2009.08.009

  5. Influenza A Virus: Cellular Entry. Reviewed

    Miyake Y, Hara Y, Umeda M, Banerjee I

    Sub-cellular biochemistry   Vol. 106   page： 387 - 401   2023

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Subcellular Biochemistry  

    The frequent emergence of pathogenic viruses with pandemic potential has posed a significant threat to human health and economy, despite enormous advances in our understanding of infection mechanisms and devising countermeasures through developing various prophylactic and therapeutic strategies. The recent coronavirus disease (COVID-19) pandemic has re-emphasised the importance of rigorous research on virus infection mechanisms and highlighted the need for our preparedness for potential pandemics. Although viruses cannot self-replicate, they tap into host cell factors and processes for their entry, propagation and dissemination. Upon entering the host cells, viruses ingeniously utilise the innate biological functions of the host cell to replicate themselves and maintain their existence in the hosts. Influenza A virus (IAV), which has a negative-sense, single-stranded RNA as its genome, is no exception. IAVs are enveloped viruses with a lipid bilayer derived from the host cell membrane and have a surface covered with the spike glycoprotein haemagglutinin (HA) and neuraminidase (NA). Viral genome is surrounded by an M1 shell, forming a “capsid” in the virus particle. IAV particles use HA to recognise sialic acids on the cell surface of lung epithelial cells for their attachment. After attachment to the cell surface, IAV particles are endocytosed and sorted into the early endosomes. Subsequently, as the early endosomes mature into late endosomes, the endosomal lumen becomes acidified, and the low pH of the late endosomes induces conformational reaggangements in the HA to initiate fusion between the endosomal and viral membranes. Upon fusion, the viral capsid disintegrates and the viral ribonucleoprotein (vRNP) complexes containing the viral genome are released into the cytosol. The process of viral capsid disintegration is called “uncoating”. After successful uncoating, the vRNPs are imported into the nucleus by importin α/β (IMP α/β), where viral replication and transcription take place and the new vRNPs are assembled. Recently, we have biochemically elucidated the molecular mechanisms of the processes of viral capsid uncoating subsequent viral genome dissociation. In this chapter, we present the molecular details of the viral uncoating process.

    DOI： 10.1007/978-3-031-40086-5_14

    Scopus

    PubMed

  6. Disrupting the HDAC6-ubiquitin interaction impairs infection by influenza and Zika virus and cellular stress pathways Reviewed

    Longlong Wang, Etori Aguiar Moreira, Georg Kempf, Yasuyuki Miyake, Blandina I. Oliveira Esteves, Amal Fahmi, Jonas V. Schaefer, Birgit Dreier, Yohei Yamauchi, Marco P. Alves, Andreas Plückthun, Patrick Matthias

    Cell Reports   Vol. 39 ( 4 ) page： 110736 - 110736   2022.4

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.celrep.2022.110736

    Web of Science

    Scopus

    PubMed

  7. A STING inhibitor suppresses EBV‐induced B cell transformation and lymphomagenesis Reviewed

    Shouhei Miyagi, Takahiro Watanabe, Yuya Hara, Masataka Arata, Md. Kamal Uddin, Keisuke Mantoku, Ken Sago, Yusuke Yanagi, Takeshi Suzuki, H. M. Abdullah Al Masud, Jun‐ichi Kawada, Shigeo Nakamura, Yasuyuki Miyake, Yoshitaka Sato, Takayuki Murata, Hiroshi Kimura

    Cancer Science   Vol. 112 ( 12 ) page： 5088 - 5099   2021.12

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    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1111/cas.15152

    Web of Science

    Scopus

    PubMed

    Other Link： https://onlinelibrary.wiley.com/doi/full-xml/10.1111/cas.15152

  8. Acetylation site specificities of lysine deacetylase inhibitors in human cells Reviewed International coauthorship

    Schölz C, Weinert BT, Wagner SA, Beli P, Miyake Y, Qi J, Jensen LJ, Streicher W, McCarthy AR, Westwood NJ, Lain S, Cox J, Matthias P, Mann M, Bradner JE, Choudhary C

    Nature biotechnology   Vol. 33 ( 4 ) page： 415 - 423   2015.4

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1038/nbt.3130

  9. Aberrant DNA polymerase alpha is excluded from the nucleus by defective import and degradation in the nucleus Reviewed

    Eichinger CS, Mizuno T, Mizuno K, Miyake Y, Yanagi K, Imamoto N, Hanaoka F

    The Journal of Biological Chemistry   Vol. 284 ( 44 ) page： 30604 - 30614   2009.10

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    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1074/jbc.M109.024760

  10. Novel splicing variant of mouse Orc1 is deficient in nuclear translocation and resistant for proteasome-mediated degradation Reviewed

    Miyake Y, Mizuno T, Yanagi K, Hanaoka F

    The Journal of Biological Chemistry   Vol. 280 ( 13 ) page： 12643 - 12652   2005.4

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    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1074/jbc.M413280200

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Books 4

  1. Influenza A Virus: Cellular Entry

    Yasuyuki Miyake, Yuya Hara, Miki Umeda, Indranil Banerjee（ Role： Joint author）

    Springer  2023 

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    Language：English

  2. インフルエンザウイルスの細胞内侵入の生化学

    三宅 康之, 山内 洋平（ Role： Joint author）

    日本生化学会  2022.6 

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    Language：English

  3. Molecular function of host factors involved in cellular entry of influenza A virus

    Yasuyuki Miyake（ Role： Sole author ,  Topics from special edition）

    the New Science Co., Ltd.  2022.5 

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    Language：Japanese Book type：Textbook, survey, introduction

  4. Purification of Unanchored Polyubiquitin Chains from Influenza Virions

    Miyake Yasuyuki, Matthias Patrick, Yamauchi Yohei（ Role： Contributor ,  Chapter 16）

    Humana Press  2018  （ ISBN:978-1-4939-8677-4 ）

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    Responsible for pages：329-342   Language：English Book type：Scholarly book

    DOI： 10.1007/978-1-4939-8678-1_16

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MISC 2

  1. 重症ウイルス感染症／ウイルス関連腫瘍を制御するための宿主因子探索と創薬応用

    佐藤好隆, 渡辺崇広, 三宅康之, 木村 宏

    最先端ナノライフシステム研究     page： 24 - 29   2022.3

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    Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (bulletin of university, research institution)  

  2. ヒストン脱アセチル化酵素HDAC6の結晶構造から明らかにされたチューブリンに対する特異性および選択的な阻害剤との結合 Invited International coauthorship

    三宅康之 , パトリック マティアス

    ライフサイエンス新着論文レビュー     2016.8

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    Authorship：Lead author   Language：Japanese   Publishing type：Article, review, commentary, editorial, etc. (trade magazine, newspaper, online media)  

    DOI： 10.7875/first.author.2016.083

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Presentations 16

  1. ENDOSOMAL DEUBIQUITINASE USP7 PROMOTES INFLUENZA VIRUS UNCOATING International conference

    Alina Rozanova, Takahiro Watanabe, Naoki Narita, Hiroshi Kimura, Yasuyuki Miyake, Yohei Yamauchi

    Negative Strand Virus Meeting 2022  2022.6.13 

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    Event date： 2022.6

    Language：English   Presentation type：Oral presentation (general)  

  2. A型インフルエンザウイルスの脱殻におけるゲノム集合体構造の役割 International coauthorship

    三宅康之、矢能彩、原優矢、Atzin Bolanos-Ceron、平賀健太郎、村田和義、内橋貴之、臼倉治郎、木村宏、Yohei Yamauchi

    第37回インフルエンザ研究者交流の会シンポジウム  2024.7.5  インフルエンザ研究者交流の会

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    Event date： 2024.7

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：松山   Country：Japan  

  3. Detailed insights on the interaction between Influenza A Virus Matrix Protein M1 and the host-cell factor Transportin-1 during the Influenza A Virus genome debundling

    Atzin Bolanos-Ceron1, Yasuyuki Miyake1,2, and Hiroshi Kimura1

    2024.7.5 

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    Event date： 2024.7

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  4. Molecular insights into the assembly and debundling of vRNPs of influenza A virus International conference

    Aya Yano, Yuya Hara, Yasuyuki Miyake, Atzin Bolanos-Ceron, Yohei Yamauchi, Hiroshi Kimura

    2023.12.6 

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    Event date： 2023.12

    Language：Japanese   Presentation type：Poster presentation  

  5. Role of endosomal acidification in RNA virus uncoating International conference

    Yuya Hara, Aya Yano, Miki Umeda, Atzin Bolanos-Ceron, Hiroshi Kimura, Yohei Yamauchi, Yasuyuki Miyake

    The 46th Annual Meeting of the Molecular Biology Society of Japan  2023.12.6 

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    Event date： 2023.12

    Language：Japanese   Presentation type：Symposium, workshop panel (public)  

  6. Host deubiquitinating enzyme USP7 and histone deacetylase HDAC6 function during uncoating of influenza A virus International conference

    The 70th Annual Meeting of the Japanese Society for Virology  2023.9.28 

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    Event date： 2023.9

    Language：Japanese   Presentation type：Poster presentation  

  7. 宿主タンパク質を介したウイルス脱殻反応の分子機構解明 International conference

    原 優矢, 三宅 康之, 山内 洋平, 木村 宏

    第69回日本ウイルス学会学術集会  2022.11.15 

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    Event date： 2022.11

    Language：Japanese   Presentation type：Oral presentation (general)  

  8. Elucidating the interactions between Influenza A Matrix Protein M1 and host cell factor Transportin-1 during vRNP debundling International conference

    2022.11.14 

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    Event date： 2022.11

    Language：English   Presentation type：Oral presentation (general)  

  9. Molecular mechanisms of uncoating in human coronavirus 229E International conference

    2022.11.15 

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    Event date： 2022.11

    Language：Japanese   Presentation type：Oral presentation (general)  

  10. USP7 promotes influenza A virus uncoating at late endosomes International conference

    Alina Rozanova, Takahiro Watanabe, Naoki Narita, Yasuyuki Miyake, Yohei Yamauchi

    2022.6.28 

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    Event date： 2022.6

    Language：Japanese   Presentation type：Oral presentation (general)  

  11. HOW INFLUENZA A VIRUS MATRIX PROTEIN M1 INTERACTS WITH HOST CELL FACTOR TRANSPORTIN-1 DURING VRNP DEBUNDLING International conference

    Atzin Ceron, Yasuyuki Miyake, Yohei Yamauchi, Hiroshi Kimura

    Negative Strand Virus Meeting 2022  2022.6.13 

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    Event date： 2022.6

    Language：English   Presentation type：Poster presentation  

  12. MOLECULAR MECHANISMS OF HUMAN CORONAVIRUS ENDOSOMAL UNCOATING International conference

    Miki Umeda, Tatsuki Tanito, Yoshitaka Sato, Mark Marsh, Hiroshi Kimura, Yohei Yamauchi, Yasuyuki Miyake

    Negative Strand Virus Meeting 2022  2022.6.12 

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    Event date： 2022.6

    Language：English   Presentation type：Poster presentation  

  13. INSIGHTS INTO INFLUENZA A VIRUS VRNP DEBUNDLING International conference

    Yuya Hara, Hiroshi Kimura, Yasuyuki Miyake, Yohei Yamauchi

    Negative Strand Virus Meeting 2022  2022.6.13 

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    Event date： 2022.6

    Language：English   Presentation type：Poster presentation  

  14. Molecular mechanism(s) for cellular entry of RNA viruses Invited

    Yasuyuki Miyake

    Premium Lecture  2021.11.18 

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    Event date： 2021.11

    Language：English   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Country：Japan  

  15. Molecular function of USP7 protein in cellular entry of influenza virus International coauthorship

    Alina Rozanova, Yasuyuki Miyake, and Yohei Yamauchi

    43th BMSJ 2020  2020.12.3 

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    Event date： 2020.12

    Language：English   Presentation type：Poster presentation  

    Venue：online   Country：Japan  

  16. 宿主因子を標的とした新規抗ウイルス薬の探索に向けて Invited International conference

    三宅康之

    中部地区医療・バイオ系シーズ発表会  2021.12.7 

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    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

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Research Project for Joint Research, Competitive Funding, etc. 2

  1. ウイルス感染における宿主因子の動態と分子機能の解明

    2021.4 - 2026.3

    科学技術振興機構  創発的研究支援事業 

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    Authorship：Principal investigator  Grant type：Competitive

  2. The HDAC6/USP7 axis in enveloped RNA virus infection

    2019.12 - 2023.3

    Yasuyuki Miyake

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    Grant type：Competitive

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KAKENHI (Grants-in-Aid for Scientific Research) 5

  1. ウイルス感染における宿主因子の動態と分子機能の解明

    2021.4 - 2026.3

    科学技術新興機構  創発的研究支援事業 

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    Authorship：Principal investigator 

  2. RNAウイルス脱殻メカニズムの分子機構解明

    2023.4 - 2026.3

    科学研究費補助金  基盤研究(C)

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    Authorship：Principal investigator  Grant type：Competitive

  3. インフルエンザウイルスとヒト免疫不全症候群ウイルスの脱殻分子メカニズムの解明

    2020.4 - 2023.3

    科学研究費補助金  基盤研究(C)

    三宅康之

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    Authorship：Principal investigator 

    　ヒトの感染症を引き起こすインフルエンザ、コロナ、エボラ、デング、ジカといった脂質二重膜に包まれたエンベロープRNAウイルスは、細胞内にエンドサイトーシスと呼ばれる食作用で取り込まれる。その後エンドソーム内部の環境が変化することでエンドソーム膜に融合し、ウイルスゲノムが細胞質内へ放出される。ウイルスの細胞内への侵入の素過程における宿主因子の機能の理解は、RNAウイルスの細胞内侵入のメカニズムだけでなく、将来的に宿主因子を標的とした新たな抗ウイルス薬の開発につながることが期待される。そこで、宿主因子とウイルス殻タンパク質の複合体を生化学的に精製し、その分子構造と相互作用メカニズムを明らかにする。

  4. The HDAC6/USP7 axis in enveloped RNA virus infection

    2020.1 - 2023.3

    Yasuyuki Miyake, Takahiro Watanabe, Yohei Yamauchi

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    Authorship：Principal investigator 

  5. Molecular mechanisms of influenza virus uncoating by ubiquitin signals

    Grant number：18J40214  2018.4 - 2021.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for JSPS Fellows

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    Authorship：Other 

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Industrial property rights 1

  1. New treatment against influenza virus

    Banerjee I , Helenius A , Matthias P , Miyake Y , Yamauchi Y

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    Application no：WO2015189816 A1  Date applied：2015

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Teaching Experience (On-campus) 16

  1. 病理病態学

    2024

  2. 生体と微生物　ウイルス学実習

    2024

  3. 生体と微生物　ウイルス学総論３

    2024

  4. 生体と微生物　ウイルス学総論３

    2023

  5. 病理病態学

    2023

  6. 生体と微生物　ウイルス学実習

    2023

  7. 基盤医学特論

    2023

    　詳細を見る

    後期エンドソームから脱殻するRNAウイルスの感染分子機構

  8. Virology 3

    2022

  9. Molecular Pathology

    2022

  10. 生体と微生物　ウイルス学実習

    2022

  11. Microorganisms and Human

    2021

  12. Molecular Pathology

    2021

  13. 生体と微生物　ウイルス学実習

    2021

  14. 病理病態学

    2020

  15. 生体と微生物　ウイルス学総論３

    2020

  16. 生体と微生物　ウイルス学実習

    2020

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Teaching Experience (Off-campus) 10

  1. ウイルス学総論（RNAウイルス）

    2024.1 （Nagoya City University）

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    Level：Undergraduate (specialized)  Country：Japan

  2. TOKURON

    2023.12 （Nagoya University Graduate School of Medicine/RIEM）

  3. ウイルス学

    2023.1 （Nagoya City University）

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    Level：Undergraduate (specialized)  Country：Japan

  4. ウイルス学 (RNAウイルス)

    2022.2 （名古屋市立大学医学部）

  5. ウイルス学

    2022.1 （Nagoya City University）

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    Level：Undergraduate (specialized)  Country：Japan

  6. 基礎医学セミナー

    2020.10 （名古屋大学医学部）

  7. Practical course in virology

    2020.4 （Nagoya University）

  8. Microorganisms and Human

    2020.4 （Nagoya University School of Medicine）

  9. Molecular Pathology

    2020.4 （Nagoya University Graduate School of Medicine）

  10. Laboratory practice for summer intern

    2018 - 2019 （University of Bristol）

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