Updated on 2022/04/12

写真a

 
ABE Naoko
 
Organization
Graduate School of Science Designated associate professor
Title
Designated associate professor
Contact information
メールアドレス

Degree 1

  1. 博士(薬科学) ( 2015.3   北海道大学 ) 

 

Papers 13

  1. Antisense oligonucleotide modified with disulfide units induces efficient exon skipping in mdx myotubes through enhanced membrane permeability and nucleus internalization.

    Hiraoka H, Shu Z, Le BT, Masuda K, Nakamoto K, Lyu F, Abe N, Hashiya F, Kimura Y, Shimizu Y, Veedu RN, Abe H

    Chembiochem : a European journal of chemical biology     2021.10

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    Language:English  

    DOI: 10.1002/cbic.202100413

    PubMed

  2. Completely Chemically Synthesized Long DNA Can be Transcribed in Human Cells

    Yamaoka Kazuki, Oikawa Ryota, Abe Naoko, Nakamoto Kosuke, Tomoike Fumiaki, Hashiya Fumitaka, Kimura Yasuaki, Abe Hiroshi

    CHEMBIOCHEM     2021.10

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  3. Phosphorothioate Modification of mRNA Accelerates the Rate of Translation Initiation to Provide More Efficient Protein Synthesis

    Kawaguchi Daisuke, Kodama Ayumi, Abe Naoko, Takebuchi Kei, Hashiya Fumitaka, Tomoike Fumiaki, Nakamoto Kosuke, Kimura Yasuaki, Shimizu Yoshihiro, Abe Hiroshi

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   Vol. 59 ( 40 ) page: 17403 - 17407   2020.9

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    Publisher:Angewandte Chemie - International Edition  

    DOI: 10.1002/anie.202007111

    Web of Science

    Scopus

    PubMed

  4. Chemically synthesized circular RNAs with phosphoramidate linkages enable rolling circle translation

    Nakamoto Kosuke, Abe Naoko, Tsuji Genichiro, Kimura Yasuaki, Tomoike Fumiaki, Shimizu Yoshihiro, Abe Hiroshi

    CHEMICAL COMMUNICATIONS   Vol. 56 ( 46 ) page: 6217 - 6220   2020.6

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    Publisher:Chemical Communications  

    DOI: 10.1039/d0cc02140g

    Web of Science

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    PubMed

  5. Translational control by secondary-structure formation in mRNA in a eukaryotic system

    Kawaguchi Daisuke, Shimizu Saaya, Abe Naoko, Hashiya Fumitaka, Tomoike Fumiaki, Kimura Yasuaki, Abe Hiroshi

    NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   Vol. 39 ( 1-3 ) page: 195 - 203   2020.2

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    Publisher:Nucleosides, Nucleotides and Nucleic Acids  

    DOI: 10.1080/15257770.2019.1671593

    Web of Science

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    PubMed

  6. Intracellular Delivery of Antisense DNA and siRNA with Amino Groups Masked with Disulfide Units

    Shu Zhaoma, Ota Azumi, Takayama Yukiya, Katsurada Yuri, Kusamori Kosuke, Abe Naoko, Nakamoto Kosuke, Tomoike Fumiaki, Tada Seiichi, Ito Yoshihiro, Nishikawa Makiya, Kimura Yasuaki, Abe Hiroshi

    CHEMICAL & PHARMACEUTICAL BULLETIN   Vol. 68 ( 2 ) page: 129 - 132   2020.2

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  7. Intracellular build-up RNAi with single-strand circular RNAs as siRNA precursors

    Kimura Yasuaki, Shu Zhaoma, Ito Mika, Abe Naoko, Nakamoto Kosuke, Tomoike Fumiaki, Shuto Satoshi, Ito Yoshihiro, Abe Hiroshi

    CHEMICAL COMMUNICATIONS   Vol. 56 ( 3 ) page: 466 - 469   2020.1

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    Publisher:Chemical Communications  

    DOI: 10.1039/c9cc04872c

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    PubMed

  8. N-6-methyl adenosine in siRNA evades immune response without reducing RNAi activity

    Imaeda Akihiro, Tomoike Fumiaki, Hayakawa Mayu, Nakamoto Kosuke, Kimura Yasuaki, Abe Naoko, Abe Hiroshi

    NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS   Vol. 38 ( 12 ) page: 972 - 979   2019.12

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    Publisher:Nucleosides, Nucleotides and Nucleic Acids  

    DOI: 10.1080/15257770.2019.1641205

    Web of Science

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    PubMed

  9. Disulfide-Unit Conjugation Enables Ultrafast Cytosolic Internalization of Antisense DNA and siRNA

    Shu Zhaoma, Tanaka Iku, Ota Azumi, Fushihara Daichi, Abe Naoko, Kawaguchi Saki, Nakamoto Kosuke, Tomoike Fumiaki, Tada Seiichi, Ito Yoshihiro, Kimura Yasuaki, Abe Hiroshi

    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION   Vol. 58 ( 20 ) page: 6611 - 6615   2019.5

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    Publisher:Angewandte Chemie - International Edition  

    DOI: 10.1002/anie.201900993

    Web of Science

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    PubMed

  10. A Covalent Inhibitor for Glutathione S-Transferase Pi (GSTP(1-1)) in Human Cells

    Shishido Yuko, Tomoike Fumiaki, Kuwata Keiko, Fujikawa Haruka, Sekido Yoshitaka, Murakami-Tonami Yuko, Kameda Tomoshi, Abe Naoko, Kimura Yasuaki, Shuto Satoshi, Abe Hiroshi

    CHEMBIOCHEM   Vol. 20 ( 7 ) page: 900 - 905   2019.4

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  11. Preparation of Circular RNA In Vitro

    Abe Naoko, Kodama Ayumi, Abe Hiroshi

    CIRCULAR RNAS: METHODS AND PROTOCOLS   Vol. 1724   page: 181 - 192   2018

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    Publisher:Methods in Molecular Biology  

    DOI: 10.1007/978-1-4939-7562-4_15

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    PubMed

  12. Chemical ligation of oligonucleotides using an electrophilic phosphorothioester

    Maruyama Hideto, Oikawa Ryota, Hayakawa Mayu, Takamori Shono, Kimura Yasuaki, Abe Naoko, Tsuji Genichiro, Matsuda Akira, Shuto Satoshi, Ito Yoshihiro, Abe Hiroshi

    NUCLEIC ACIDS RESEARCH   Vol. 45 ( 12 ) page: 7042 - 7048   2017.7

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    Publisher:Nucleic Acids Research  

    DOI: 10.1093/nar/gkx459

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  13. Rolling Circle Translation of Circular RNA

    ABE Naoko, ABE Hiroshi

    Seibutsu Butsuri   Vol. 57 ( 1 ) page: 5 - 10   2017

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    Publisher:The Biophysical Society of Japan General Incorporated Association  

    <p>In an <i>E. coli</i> cell-free translation system, we found that a circular RNA containing an infinite open reading frame produced more translation product than its linear counterpart by two orders of magnitude, because a ribosome can work more effectively towards the elongation on circular RNA than it can on linear RNA. We then tested circular RNAs containing an infinite open reading frame could be translated in eukaryotic systems, in the absence of any particular element for internal ribosome entry, a poly-A tail, or a cap structure. We found that the circular RNAs also produced long peptides in eukaryotic translation systems, possibly owing to the rolling circle amplification mechanism.</p>

    DOI: 10.2142/biophys.57.005

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KAKENHI (Grants-in-Aid for Scientific Research) 1

  1. 真核系における環状RNA翻訳反応の機構解析

    Grant number:18K14357  2018.4 - 2021.3

    若手研究

    阿部 奈保子

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    Authorship:Principal investigator 

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    真核生物のmRNAは5’末端にキャップ構造、3’末端側にアデニンが連続したポリA鎖という特殊な構造を持つ。キャップに結合する翻訳開始因子であるeIF4EにeIF4Gが結合し、ポリA鎖に結合するタンパク質であるPABPがeIF4Gと相互作用する。このmRNAの環状複合体形成は、mRNAの安定化やリボソームのリサイクル促進による翻訳反応効率化に寄与することが知られている。本研究ではこの環状構造形成を2次元的に模倣することでRNAの翻訳効率を上昇させられないかと考えた。2つのUTR間で塩基対を形成しうるmRNA, ds_Rlucを設計・合成し、2つのコントロール配列ss_Rluc, cap-polyA_Rlucととも真核生物無細胞翻訳系(ウサギ網状赤血球溶解液)にてこれらを翻訳し、ルシフェラーゼアッセイ法によりその効率を比較した。その結果、ss_Rlucに比較し、ds_Rlucからはおよそ3倍の翻訳産物が生じた。加えて、ds_Rlucは、5‘キャップ構造及びポリA鎖を持つcap-polyA_Rlucとほぼ同等の翻訳効率を示した。続いてds_Rluc,およびss_Rlucの翻訳液中での安定性を評価した結果、安定性は同程度であり両者に差はなかった。これらの実験結果から、UTR間で塩基対を形成することで環状構造を取りうるds_Rlucの遺伝子発現効率が上昇したこと、およびその効率上昇はRNAの安定性向上に起因しないことが分かった。期待したとおり、mRNAが環状構造を取ることによりリボソームのリサイクリングが促進された可能性が示唆された。本研究結果を学術雑誌 Nucleosides, Nucleotides and Nucleic Acids, 1-9(2019) に発表した。
    環状RNA上の連続的翻訳反応に必要とされる配列要因の解明が、予期していたより困難であった。加えて、長鎖直鎖状RNA(1000ヌクレオチド程度)の酵素を用いた環状化反応を試みた際、条件の検討を行っても困難であった。
    長鎖RNA(1000ヌクレオチド程度)の効率的合成法、特にリガーゼを用いた連結方法の確立を試みる。これにより、近年多くの存在が明らかになってきた、内因性環状RNAの機能解明に貢献する。