Updated on 2023/10/12

写真a

 
HASHIYA Fumitaka
 
Organization
Research Center for Materials Science Assistant Professor
Graduate School
Graduate School of Science
Title
Assistant Professor

Degree 1

  1. PhD （ 2019.3   Kyoto University ） 

To the head of Degree.▲

Research Interests 4

  1. nucleosome

  2. 生化学

  3. 核酸化学

  4. ケミカルバイオロジー

To the head of Research Interests.▲

Research Areas 1

  1. Life Science / Molecular biology

To the head of Research Areas.▲

Research History 2

  1. Nagoya University   Assistant Professor

    2019.7

  2. Nagoya University   Researcher

    2019.4 - 2019.7

To the head of Research History.▲

Education 3

  1. Kyoto University

    2016.4 - 2019.3

  2. Kyoto University

    2014.4 - 2016.3

  3. Kyoto University

    2010.4 - 2014.3

To the head of Education.▲

Professional Memberships 5

  1. 日本分子生物学会   会員

    2022.10

  2. 日本光生物・光医学会

  3. 日本核酸学会

  4. 日本ケミカルバイオロジー学会

  5. 日本化学会

To the head of Professional Memberships.▲

Awards 2

  1. 奨励賞

    2021.1   日本光医学・光生物学会   5-ブロモウラシルと光反応を利用した新規ヌクレオソーム形成部位の特定手法

    橋谷文貴、杉山弘、阿部洋

    　More details

    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

  2. ISNAC Outstanding Oral Presentation Award for Young Scientist in 2021 (Ohtsuka Award)

    2021.11   ISNAC   Chemically modified PCR primer aiming accurate and efficient DNA assembly

To the head of Awards.▲
 

Papers 24

  1. Topological capture of mRNA for silencing gene expression Reviewed International journal

    Lyu Fangjie, Tomita Takashi, Abe Naoko, Hiraoka Haruka, Hashiya Fumitaka, Nakashima Yuko, Kajihara Shiryu, Tomoike Fumiaki, Shu Zhaoma, Onizuka Kazumitsu, Kimura Yasuaki, Abe Hiroshi

    CHEMICAL COMMUNICATIONS     2023.9

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1039/d2cc06189

    Web of Science

  2. The effect of γ phosphate modified deoxynucleotide substrates on PCR activity and fidelity. Reviewed International journal

    Fumitaka Hashiya, Hirotaka Murase, Akash Chandela, Haruka Hiraoka, Masahito Inagaki, Yuko Nakashima, Naoko Abe, Mayu Nakamura, Goro Terai, Yasuaki Kimura, Kaori Ando, Natsuhisa Oka, Kiyoshi Asai, Hiroshi Abe

    Chembiochem : a European journal of chemical biology   Vol. 24 ( 14 ) page： e202200572   2023.6

    　More details

    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Controlling PCR fidelity is an important issue for molecular biology and high-fidelity PCR is essential for gene cloning. In general, fidelity control is achieved by protein engineering of polymerases. In contrast, only a few studies have reported controlling fidelity using chemically modified nucleotide substrates. In this report, we synthesized nucleotide substrates possessing a modification on Pγ and evaluated the effect of this modification on PCR fidelity. One of the substrates, nucleotide tetraphosphate, caused a modest decrease in Taq DNA polymerase activity and the effect on PCR fidelity was dependent on the type of mutation. The use of deoxyadenosine tetraphosphate enhanced the A:T→G:C mutation dramatically, which is common when using Taq polymerase. Conversely, deoxyguanosine tetraphosphate (dG4P) suppressed this mutation but increased the G:C→A:T mutation during PCR. Using an excess amount of dG4P suppressed both mutations successfully and total fidelity was improved.

    DOI： 10.1002/cbic.202200572

    Web of Science

    Scopus

    PubMed

  3. Cap analogs with a hydrophobic photocleavable tag enable facile purification of fully capped mRNA with various cap structures

    Inagaki Masahito, Abe Naoko, Li Zhenmin, Nakashima Yuko, Acharyya Susit, Ogawa Kazuya, Kawaguchi Daisuke, Hiraoka Haruka, Banno Ayaka, Meng Zheyu, Tada Mizuki, Ishida Tatsuma, Lyu Pingxue, Kokubo Kengo, Murase Hirotaka, Hashiya Fumitaka, Kimura Yasuaki, Uchida Satoshi, Abe Hiroshi

    NATURE COMMUNICATIONS   Vol. 14 ( 1 ) page： 2657   2023.5

    　More details

    Language：English   Publisher：Nature Communications  

    Starting with the clinical application of two vaccines in 2020, mRNA therapeutics are currently being investigated for a variety of applications. Removing immunogenic uncapped mRNA from transcribed mRNA is critical in mRNA research and clinical applications. Commonly used capping methods provide maximum capping efficiency of around 80–90% for widely used Cap-0- and Cap-1-type mRNAs. However, uncapped and capped mRNA possesses almost identical physicochemical properties, posing challenges to their physical separation. In this work, we develop hydrophobic photocaged tag-modified cap analogs, which separate capped mRNA from uncapped mRNA by reversed-phase high-performance liquid chromatography. Subsequent photo-irradiation recovers footprint-free native capped mRNA. This approach provides 100% capping efficiency even in Cap-2-type mRNA with versatility applicable to 650 nt and 4,247 nt mRNA. We find that the Cap-2-type mRNA shows up to 3- to 4-fold higher translation activity in cultured cells and animals than the Cap-1-type mRNA prepared by the standard capping method.

    DOI： 10.1038/s41467-023-38244-8

    Web of Science

    Scopus

    PubMed

  4. Development of an Electric Pegboard (e-Peg) for Hand Dexterity Improvement and Cognitive Rehabilitation: A Preliminary Study Reviewed

    Okahashi Sayaka, Sakamoto Kenta, Hashiya Fumitaka, Kumasaka Keisuke, Yamaguchi Taro, Seiyama Akitoshi, Utsumi Jun

    Advanced Biomedical Engineering   Vol. 12 ( 0 ) page： 81 - 90   2023

    　More details

    Language：English   Publisher：Japanese Society for Medical and Biological Engineering  

    <p>Fine motor dysfunction and cognitive impairments commonly develop after stroke, which greatly impact the daily lives of patients. In current occupational therapy, hand dexterity and cognitive functions are evaluated individually (e.g., by manipulation of small objects with fingers, or a paper-and-pencil test), which is insufficient for therapists to grasp the total ability of combined dexterity and cognition in everyday situations. Additionally, the traditional methods require a tester to measure the completion time manually and tend to be monotonous for patients. These problems would be solved using technology. This study aimed to develop a new electric pegboard (e-Peg) prototype and to investigate preliminary utility in healthy adults. The system judges the peg insertion accuracy based on magnetism and records the time course and scores, which are linked to human object manipulation ability. The e-Peg executes three types of tasks: a basic color matching task (BT), a color comparison task using a pattern sheet (CT), and a visual memory task (MT), with one/two-color sample patterns. Six older and nine younger healthy adults performed the e-Peg tasks, functional tests, and responded to questionnaires. As a result, the number of correct answers in a bicolor symmetrical MT were significantly greater in the younger group than in the older group. The older group required a significantly longer completion time for BT and CT than the younger group. Significant correlations were found between one-color BT/CT and dexterity tests, between bicolor BT/CT and dexterity/cognitive tests, and between a bicolor MT and a cognitive test. Questionnaire results revealed that participants regarded BT/CT as easy/interesting tasks, whereas MT was considered a difficult/challenging task. In conclusion, our e-Peg is potentially a useful rehabilitation device that facilitates many tasks related to hand manipulation and attention/executive functions, and a valuable tool for personalized therapy.</p>

    DOI： 10.14326/abe.12.81

    Web of Science

    Scopus

    CiNii Research

  5. A 2'-modified uridine analog, 2'-O-(methylthiomethoxy)methyl uridine, for siRNA applications Reviewed International journal

    Lyu Fangjie, Seongjin An, Yoshiaki Kobayashi, Kohei Nomura, Rintaro Baba, Naoko Abe, Haruka Hiraoka, Fumitaka Hashiya, Zhaoma Shu, Kumiko Ui-Tei, Yasuaki Kimura, Hiroshi Abe

    Bioorganic &amp; Medicinal Chemistry Letters   Vol. 74   page： 128939 - 128939   2022.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    DOI： 10.1016/j.bmcl.2022.128939

    Web of Science

    Scopus

    PubMed

  6. Cap analogs with a hydrophobic photocleavable tag enable facile purification of fully capped mRNA with various cap structures.

    Masahito Inagaki, Naoko Abe, Zhenmin Li, Yuko Nakashima, Susit Acharyya, Kazuya Ogawa, Daisuke Kawaguchi, Haruka Hiraoka, Ayaka Banno, Zheyu Meng, Mizuki Tada, Tatsuma Ishida, Pingxue Lyu, Kengo Kokubo, Hirotaka Murase, Fumitaka Hashiya, Yasuaki Kimura, Satoshi Uchida, Hiroshi Abe

        2022.9

    　More details

    Publisher：American Chemical Society (ACS)  

    Removing immunogenic uncapped mRNA from in vitro transcribed mRNA is critical in mRNA research and clinical applications. Commonly used capping methods provide a maximum capping efficiency of around 80-90% for widely used Cap-0- and Cap-1-type mRNAs. However, uncapped and capped mRNA possesses almost identical physicochemical properties, posing challenges to their physical separation. Herein, we developed hydrophobic photocaged tag-modified cap analogs, which separated capped mRNA from uncapped mRNA by reversed-phase HPLC. Subsequent photo-irradiation recovers footprint-free native capped mRNA. This approach provided 100% capping efficiency even in Cap-2-type mRNA with versatility applicable to 650 nt and 4,247 nt mRNA. The Cap-2-type mRNA showed up to 3 to 4-fold higher translational activity in cultured cells and animals than mRNA prepared by the standard capping method. Notably, the purification process simultaneously removed immunogenic double-stranded mRNA, another major contaminant of in vitro transcribed mRNA, drastically reducing mRNA immunogenicity in cultured cells.

    DOI： 10.26434/chemrxiv-2022-vfjt6

  7. Development of Fluorophosphoramidate as a Biocompatibly Transformable Functional Group and its Application as a Phosphate Prodrug for Nucleoside Analogs. Reviewed International journal

    Yoshida, Y, Zheng, T, Tanabe, W, Tomoike, F, Hashiya, F, Suzuki, T, Hirota, S, Saiki, Y, Horii, A, Hirayama, A, Soga, T, Kimura, Y, Abe, H

    ChemMedChem   Vol. 17 ( 17 ) page： e202200188   2022.9

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Synthetic phosphate-derived functional groups are important for controlling the function of bioactive molecules in vivo. Herein we describe the development of a new type of biocompatible phosphate analog, a fluorophosphoramidate (FPA) functional group that has characteristic P-F and P-N bonds. We found that FPA with a primary amino group was relatively unstable in aqueous solution and was converted to a monophosphate, while FPA with a secondary amino group was stable. Furthermore, by improving the molecular design of FPA, we developed a reaction in which a secondary amino group is converted to a primary amino group in the intracellular environment and clarified that the FPA group functions as a phosphate prodrug of nucleoside. Various FPA-gemcitabine derivatives were synthesized and their toxicity to cancer cells were evaluated. One of the FPA-gemcitabine derivatives showed superior toxicity compared with gemcitabine and its ProTide prodrug, which methodology is widely used in various nucleoside analogs, including anti-cancer and anti-virus drugs.

    DOI： 10.1002/cmdc.202200188

    Web of Science

    Scopus

    PubMed

  8. Complete Chemical Synthesis of Minimal Messenger RNA by Efficient Chemical Capping Reaction. Reviewed International journal

    Naoko Abe, Akihiro Imaeda, Masahito Inagaki, Zhenmin Li, Daisuke Kawaguchi, Kaoru Onda, Yuko Nakashima, Satoshi Uchida, Fumitaka Hashiya, Yasuaki Kimura, Hiroshi Abe

    ACS chemical biology   Vol. 17 ( 6 ) page： 1308 - 1314   2022.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Site-specific chemical modification of mRNA can improve its translational efficiency and stability. For this purpose, it is desirable to develop a complete chemical synthesis method for chemically modified mRNA. The key is a chemical reaction that introduces a cap structure into the chemically synthesized RNA. In this study, we developed a fast and quantitative chemical capping reaction between 5'-phosphorylated RNA and N7-methylated GDP imidazolide in the presence of 1-methylimidazole in the organic solvent dimethyl sulfoxide. It enabled quantitative preparation of capping RNA within 3 h. We prepared chemically modified 107-nucleotide mRNAs, including N6-methyladenosine, insertion of non-nucleotide linkers, and 2'-O-methylated nucleotides at the 5' end and evaluated their effects on translational activity in cultured HeLa cells. The results showed that mRNAs with non-nucleotide linkers in the untranslated regions were sufficiently tolerant to translation and that mRNAs with the Cap_2 structure had higher translational activity than those with the Cap_0 structure.

    DOI： 10.1021/acschembio.1c00996

    Web of Science

    Scopus

    PubMed

  9. Antisense Oligonucleotide Modified with Disulfide Units Induces Efficient Exon Skipping in mdx Myotubes through Enhanced Membrane Permeability and Nucleus Internalization Reviewed International journal

    Hiraoka, Haruka, Shu, Zhaoma, Tri Le, Bao, Masuda, Keiko, Nakamoto, Kosuke, Fangjie, Lyu, Abe, Naoko, Hashiya, Fumitaka, Kimura, Yasuaki, Shimizu, Yoshihiro, Veedu, Rakesh N., Abe, Hiroshi

    Chembiochem   Vol. 22 ( 24 ) page： 3437 - 3442   2021.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    We have found that antisense oligonucleotides and siRNA molecules modified with repeat structures of disulfide units can be directly introduced into the cytoplasm and exhibit a suppressive effect on gene expression. In this study, we analyzed the mechanism of cellular uptake of these membrane-permeable oligonucleotides (MPONs). Time-course analysis by confocal microscopy showed that the uptake of MPONs from the plasma membrane to the cytoplasm reached 50 % of the total uptake in about 5 min. In addition, analysis of the plasma membrane proteins to which MPONs bind, identified several proteins, including voltage-dependent anion channel. Next, we analyzed the behavior of MPONs in the cell and found them to be abundant in the nucleus as early as 24 h after addition with the amount increasing further after 48 and 72 h. The amount of MPONs was 2.5-fold higher than that of unmodified oligonucleotides in the nucleus after 72 h. We also designed antisense oligonucleotides and evaluated the effect of MPONs on mRNA exon skipping using DMD model cells; MPONs caused exon skipping with 69 % efficiency after 72 h, which was three times higher than the rate of the control. In summary, the high capacity for intracytoplasmic and nuclear translocation of MPONs is expected to be useful for therapeutic strategies targeting exon skipping.

    DOI： 10.1002/cbic.202100413

    Web of Science

    Scopus

    PubMed

  10. Complete chemical synthesis of long DNA transcriptable in human cells

    Kazuki Yamaoka, Ryota Oikawa, Naoko Abe, Kosuke Nakamoto, Fumiaki Tomoike, Fumitaka Hashiya, Yasuaki Kimura, Hiroshi Abe

    ChemBioChem   Vol. 22 ( 23 ) page： 3273 - 3276   2021.12

    　More details

    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1002/cbic.202100312

    Web of Science

    Scopus

    PubMed

  11. Variety of nucleotide polymerase mutants aiming to synthesize modified RNA

    Sana Ohashi, Fumitaka Hashiya, Hiroshi Abe

    ChemBioChem   Vol. 22 ( 14 ) page： 2398 - 2406   2021.7

    　More details

    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Wiley  

    DOI： 10.1002/cbic.202100004

    Web of Science

    Scopus

    PubMed

  12. A novel method for locating nucleosomes by exploiting 5-bromouracil, pyrene-modified histone, and photoirradiation Reviewed

    Fumitaka Hashiya, Hiroshi Sugiyama

    Photomedicine and Photobiology   Vol. 42   page： 13 - 16   2021

    　More details

    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

  13. Direct Observation of Dynamic Interactions between Orientation-Controlled Nucleosomes in a DNA Origami Frame. International journal

    Yihong Feng, Fumitaka Hashiya, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo

    Chemistry (Weinheim an der Bergstrasse, Germany)   Vol. 26 ( 66 ) page： 15282 - 15289   2020.11

    　More details

    Language：Japanese   Publishing type：Research paper (scientific journal)  

    The nucleosome is one of the most fundamental units involved in gene expression and consequent cell development, differentiation, and expression of cell functions. We report here a method to place reconstituted nucleosomes into a DNA origami frame for direct observation using high-speed atomic-force microscopy (HS-AFM). By using this method, multiple nucleosomes can be incorporated into a DNA origami frame and real-time movement of nucleosomes can be visualized. The arrangement and conformation of nucleosomes and the distance between two nucleosomes can be designed and controlled. In addition, four nucleosomes can be placed in a DNA frame. Multiple nucleosomes were well accessible in each conformation. Dynamic movement of the individual nucleosomes were precisely monitored in the DNA frame, and their assembly and interaction were directly observed. Neither mica surface modification nor chemical fixation of nucleosomes is used in this method, meaning that the DNA frame not only holds nucleosomes, but also retains their natural state. This method offers a promising platform for investigating nucleosome interactions and for studying chromatin structure.

    DOI： 10.1002/chem.202003071

    Web of Science

    Scopus

    PubMed

  14. Phosphorothioate Modification of mRNA Accelerates the Rate of Translation Initiation to Provide More Efficient Protein Synthesis. International journal

    Daisuke Kawaguchi, Ayumi Kodama, Naoko Abe, Kei Takebuchi, Fumitaka Hashiya, Fumiaki Tomoike, Kosuke Nakamoto, Yasuaki Kimura, Yoshihiro Shimizu, Hiroshi Abe

    Angewandte Chemie (International ed. in English)   Vol. 59 ( 40 ) page： 17403 - 17407   2020.9

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Messenger RNAs (mRNAs) with phosphorothioate modification (PS-mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were prepared, and the translation reaction was evaluated using an E. coli cell-free translation system. Protein synthesis from PS-mRNA increased in 12 of 15 patterns when compared with that of unmodified mRNA. The protein yield increased 22-fold when the phosphorothioate modification at A/C sites was introduced into the region from the 5'-end to the initiation codon. Single-turnover analysis of PS-mRNA translation showed that phosphorothioate modification increases the number of translating ribosomes, thus suggesting that the rate of translation initiation (rate of ribosome complex formation) is positively affected by the modification. The method provides a new strategy for improving translation by using non-natural mRNA.

    DOI： 10.1002/anie.202007111

    Web of Science

    Scopus

    PubMed

  15. A synthetic transcription factor pair mimic for precise recruitment of an epigenetic modifier to the targeted DNA locus. International journal

    Zutao Yu, Mengting Ai, Soumen K Samanta, Fumitaka Hashiya, Junichi Taniguchi, Sefan Asamitsu, Shuji Ikeda, Kaori Hashiya, Toshikazu Bando, Ganesh N Pandian, Lyle Isaacs, Hiroshi Sugiyama

    Chemical communications (Cambridge, England)   Vol. 56 ( 15 ) page： 2296 - 2299   2020.2

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    We developed an epigenetically active, cooperative DNA binding transcription factor platform assisted by cucurbit[7]uril (CB7) host-guest modules. This new type of molecule termed ePIP-HoGu not only mimics the operation of transcription factors as a pair but also recruits the epigenetic modifier to a particular DNA locus.

    DOI： 10.1039/c9cc09608f

    PubMed

  16. Translational control by secondary-structure formation in mRNA in a eukaryotic system. International journal

    Daisuke Kawaguchi, Saaya Shimizu, Naoko Abe, Fumitaka Hashiya, Fumiaki Tomoike, Yasuaki Kimura, Hiroshi Abe

    Nucleosides, nucleotides & nucleic acids   Vol. 39 ( 1-3 ) page： 195 - 203   2020.2

    　More details

    Language：Japanese   Publishing type：Research paper (scientific journal)  

    Eukaryotic mRNA has a cap structure at the 5' end and a poly(A) tail at the 3' end. The cap and poly(A) tail form a complex with multiple translation factors, and mRNA forms a circularized structure called a closed-loop model. This circularized structure reportedly not only stabilizes mRNA but also promotes ribosome recycling during translation, which improves translation efficiency. We designed an artificial mRNA that forms a circularized structure without a cap structure and poly(A) tail and found that its translational efficiency was improved compared with that of a sequence without the circularized structure in a eukaryotic translation system.

    DOI： 10.1080/15257770.2019.1671593

    Web of Science

    Scopus

    PubMed

  17. Electron injection from mitochondrial transcription factor A to DNA associated with thymine dimer photo repair. Reviewed International journal

    Fumitaka Hashiya, Shinji Ito, Hiroshi Sugiyama

    Bioorganic & medicinal chemistry   Vol. 27 ( 2 ) page： 278 - 284   2019.1

    　More details

    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    Electron transfer through π-stacked arrays of double-stranded DNA contributes to the redox chemistry of bases, including guanine oxidation and thymine-thymine dimer repair by photolyase. 5-Bromouracil is an attractive photoreactive thymine analogue that can be used to investigate electron transfer in DNA, and is a useful probe for protein-DNA interaction analysis. In the present study using BrU we found that UV irradiation facilitated electron injection from mitochondrial transcription factor A into DNA. We also observed that this electron injection could lead to repair of a thymine-thymine dimer.

    DOI： 10.1016/j.bmc.2018.11.044

    PubMed

  18. Approach to the Investigation of Nucleosome Structure by Using the Highly Emissive Nucleobase th dG-tC FRET Pair. International journal

    Ji Hoon Han, Soyoung Park, Fumitaka Hashiya, Hiroshi Sugiyama

    Chemistry (Weinheim an der Bergstrasse, Germany)   Vol. 24 ( 64 ) page： 17091 - 17095   2018.11

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    A distance- and orientation-factor-dependent FRET system is a useful and attractive approach to the investigation of the conformational dynamics of nucleosomes. In this study, the application of the highly emissive nucleobase th dG-tC FRET pair to 601 nucleosomes is reported. It was found that the th dG-tC FRET pair was successfully incorporated to 145 bp 601 sequences, and different FRET efficiencies were obtained for the designated donor and acceptor positions in the nucleosome.

    DOI： 10.1002/chem.201803382

    PubMed

  19. Direct Observation of H3-H4 Octasome by High-Speed AFM. International journal

    Tingting Zou, Fumitaka Hashiya, Yulei Wei, Zutao Yu, Ganesh N Pandian, Hiroshi Sugiyama

    Chemistry (Weinheim an der Bergstrasse, Germany)   Vol. 24 ( 60 ) page： 15998 - 16002   2018.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    Despite evidence that histone H3 and H4 proteins may act as the precursor for orientating the DNA sequence to form nucleosome structures, there is no direct evidence of the formed compact structure. Here, it is demonstrated that a histone H3-H4 octasome could be constructed without the involvement of histone H2A-H2B under in vitro reconstitution conditions. Atomic force microscopy was used to obtain the first direct observation of the octasome structure, which exhibited a similar core-protein size as that of a nucleosome but with a shorter core histone-binding DNA region. The octasome also displayed a one-step histone-dissociation pattern under heat treatment, distinct micrococcal nuclease and peplomycin accessibility, which suggests a different wrapping pattern to that in nucleosomes.

    DOI： 10.1002/chem.201804010

    PubMed

  20. Biomimetic Artificial Epigenetic Code for Targeted Acetylation of Histones. International journal

    Junichi Taniguchi, Yihong Feng, Ganesh N Pandian, Fumitaka Hashiya, Takuya Hidaka, Kaori Hashiya, Soyoung Park, Toshikazu Bando, Shinji Ito, Hiroshi Sugiyama

    Journal of the American Chemical Society   Vol. 140 ( 23 ) page： 7108 - 7115   2018.6

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    While the central role of locus-specific acetylation of histone proteins in eukaryotic gene expression is well established, the availability of designer tools to regulate acetylation at particular nucleosome sites remains limited. Here, we develop a unique strategy to introduce acetylation by constructing a bifunctional molecule designated Bi-PIP. Bi-PIP has a P300/CBP-selective bromodomain inhibitor (Bi) as a P300/CBP recruiter and a pyrrole-imidazole polyamide (PIP) as a sequence-selective DNA binder. Biochemical assays verified that Bi-PIPs recruit P300 to the nucleosomes having their target DNA sequences and extensively accelerate acetylation. Bi-PIPs also activated transcription of genes that have corresponding cognate DNA sequences inside living cells. Our results demonstrate that Bi-PIPs could act as a synthetic programmable histone code of acetylation, which emulates the bromodomain-mediated natural propagation system of histone acetylation to activate gene expression in a sequence-selective manner.

    DOI： 10.1021/jacs.8b01518

    PubMed

  21. A novel detection technique of polyamide binding sites by photo-induced electron transfer in (Br)U substituted DNA. Invited Reviewed

    Chemical Communications   Vol. 51 ( 77 ) page： 14485 - 14488   2015.10

    　More details

    Language：English  

  22. Locating the uracil-5-yl radical formed upon photoirradiation of 5-bromouracil-substituted DNA. Invited Reviewed

    Nucleic acids research   Vol. 42 ( 22 ) page： 13469 - 13473   2014.12

    　More details

    Authorship：Lead author   Language：English  

  23. Sequence-specific DNA alkylation and transcriptional inhibition by long-chain hairpin pyrrole-imidazole polyamide-chlorambucil conjugates targeting CAG/CTG trinucleotide repeats. Invited Reviewed

    Bioorganic & medicinal chemistry.   Vol. 22 ( 17 ) page： 4646 - 4657   2014.9

    　More details

    Language：English  

  24. Sequence-specific electron injection into DNA from an intermolecular electron donor. Reviewed

    Hironobu Morinaga, Tomohiro Takenaka, Fumitaka Hashiya, Seiichiro Kizaki, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama.

    Nucleic acids research   Vol. 41 ( 8 ) page： 4724 - 4728   2013.4

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

▼display all

To the head of Papers.▲

Presentations 50

  1. Chemically modified PCR primer aiming accurate and efficient DNA assembly International conference

    Fumitaka Hashiya, Kaoru Onda, Kohei Nomura, Gao Yiuno, Hirotaka Murase, Kosuke Nakamoto, Masahito Inagaki, Haruka Hiraoka, Naoko Abe, Yasuaki Kimura, Natsuhisa Oka, Goro Terai, Kiyoshi Asai, Hiroshi Abe

    2021.11.11 

    　More details

    Event date： 2021.11

    Language：English   Presentation type：Oral presentation (general)  

    Country：Japan  

  2. 5-ブロモウラシルと光反応を利用した新規ヌクレオソーム形成部位の特定手法 Invited

    橋谷文貴、杉山弘、阿部洋

    第４２回日本光医学・光生物学会  2021.1.22 

    　More details

    Event date： 2021.1

    Language：Japanese   Presentation type：Oral presentation (invited, special)  

  3. 光照射による細胞内ビルドアップ制御を志向したポスト切断PCRプライマーの開発

    平岡陽花、村瀬裕貴、阿部奈保子、吉田祐希、橋谷文貴、阿部洋

    日本薬学会第143年会 

    　More details

    Event date： 2023.3

    Presentation type：Oral presentation (general)  

  4. 光反応性保護基をもつキャップ化合物を利用した高純度キャップ化mRNA調製法の開発

    ○阿部 奈保子、稲垣 雅仁、Li Zhenmin、中嶋 裕子、Acharyya Susit、平岡 陽花、橋谷 文貴、木村 康明、内田 智士、阿部 洋

    日本薬学会第143年会 

    　More details

    Event date： 2023.3

    Presentation type：Poster presentation  

  5. 人工mＲＮＡ調製を可能にする新規化学リン酸化試薬の開発

    乙竹真美、恩田馨、稲垣雅仁、阿部奈保子、橋谷文貴、木村康明、阿部洋

    日本薬学会第143年会 

    　More details

    Event date： 2023.3

    Presentation type：Oral presentation (general)  

  6. Genome scale DNA assembly using modified primers

    〇橋谷 文貴・恩田 馨・野村 浩平・Gao Yiuno・村瀬 裕貴・中本 航介・稲垣 雅仁・平岡 陽花・阿部 奈保子・木村 康明・岡 夏央・寺井 悟朗・浅井 潔・阿部 洋

    　More details

    Event date： 2023.3

    Presentation type：Oral presentation (general)  

  7. 完全キャップ化メッセンジャーRNAの製造を可能にする共転写用PureCapアナログの開発

    ○稲垣 雅仁1・阿部 奈保子1・Zhenmin Li1・中嶋 裕子1,2・Susit Acharyya1・小川 和哉1・川口 大輔1・平岡 陽花1・坂野 文香1・Zheyu Meng1・多田 瑞紀1・石田 竜真1・Pingxue Lyu1・小久保 建吾1・村瀬 裕貴1・橋谷 文貴2・木村 康明1・内田 智士3,4・阿部 洋

    日本化学会 第103回春季年会 

    　More details

    Event date： 2023.3

    Presentation type：Oral presentation (general)  

  8. Development of a DNA assembly technology using modified nucleic acids

    　More details

    Event date： 2023.3

    Presentation type：Oral presentation (general)  

  9. 化学修飾プライマーとライゲーション反応を用いたランダム配列を有するDNAライブラリーの構築

    ○高橋 南帆、野村 浩平、恩田 馨、鈴木 大輔、村瀬 裕貴、稲垣 雅仁、平岡 陽花、阿部 奈保子、橋谷 文貴、木村 康明、阿部 洋

    日本化学会 第103回春季年会 

    　More details

    Event date： 2023.3

    Presentation type：Oral presentation (general)  

  10. 化学修飾プライマーを用いた任意の接着末端作成と長鎖DNAの連結

    橋谷 文貴・恩田 馨・野村 浩平・Gao Yiuno・村瀬 裕貴・中本 航介・稲垣 雅仁・平岡 陽花・阿部 奈保子・木村 康明・岡 夏央・寺井 悟朗・浅井 潔・阿部 洋

    第45回日本分子生物学会年会 

    　More details

    Event date： 2022.11 - 2022.12

    Presentation type：Poster presentation  

  11. 長鎖DNAの細胞内ビルドアップを志向した、細胞内光切断が可能なポスト切断PCRプライマーの開発

    平岡陽花、村瀬裕貴、阿部奈保子、吉田祐希、橋谷文貴、阿部洋

    第45回日本分子生物学会年会 

    　More details

    Event date： 2022.11 - 2022.12

    Presentation type：Poster presentation  

  12. フルオロリン酸アミデート基を用いた新規リン酸プロドラッグの開発

    吉田 祐希・Ti Zheng・田辺 航・友池 史明・橋谷 文貴・廣田 嵩人・齋木 由利子・堀井 明・木村 康明・阿部 洋

    第39回メディシナルケミストリーシンポジウム 

    　More details

    Event date： 2022.11

    Presentation type：Poster presentation  

  13. 新規２′修飾核酸によるｓｉＲＮＡの標的特異性の向上

    馬場 麟太郎・野村浩平・阿部 奈保子・安 成鎮・小林 芳明・程 久美子・橋谷 文貴・木村 康明・阿部 洋

    第39回メディシナルケミストリーシンポジウム 

    　More details

    Event date： 2022.11

    Presentation type：Poster presentation  

  14. 化学修飾プライマーを利用したゲノムスケールDNAアセンブリ

    橋谷 文貴・恩田 馨・野村 浩平・Gao Yiuno・村瀬 裕貴・中本 航介・稲垣 雅仁・平岡 陽花・阿部 奈保子・木村 康明・岡 夏央・寺井 悟朗・浅井 潔・阿部 洋

    第17回無細胞生命科学研究会 

    　More details

    Event date： 2022.11

    Presentation type：Oral presentation (general)  

  15. Complete Chemical Synthesis of Minimal Messenger RNA by Efficient Chemical Capping Reaction International conference

    　More details

    Event date： 2022.11

    Presentation type：Poster presentation  

  16. 化学修飾プライマーを利用した突出末端作成とDNA連結

    橋谷 文貴・恩田 馨・野村 浩平・Gao Yiuno・村瀬 裕貴・中本 航介・稲垣 雅仁・平岡 陽花・阿部 奈保子・木村 康明・岡 夏央・寺井 悟朗・浅井 潔・阿部 洋

    中部化学関係学協会支部連合秋季大会 

    　More details

    Event date： 2022.11

    Presentation type：Oral presentation (general)  

  17. フルオロリン酸アミデート基を用いた新規リン酸プロドラッグの開発 International conference

    吉田 祐希・Ti Zheng・田辺 航・友池 史明・橋谷 文貴・廣田 嵩人・齋木 由利子・堀井 明・木村 康明・阿部 洋

    ISNAC2022 第49回国際化学シンポジウム 日本核酸化学会第6回年会 

    　More details

    Event date： 2022.11

    Presentation type：Poster presentation  

  18. Complete Chemical Synthesis of mRNA by Chemical Capping Reaction International conference

    Yasuaki Kimura, Naoko Abe, Akihiro Imaeda, Masahito Inagaki, Fumitaka Hashiya, Satoshi Uchida, Hiroto Iwai, Masakazu Honma, Junichiro Yamamoto, Hiroshi Abe

    　More details

    Event date： 2022.11

    Presentation type：Oral presentation (general)  

  19. 3'チオ化核酸の合成と新規長鎖オリゴ核酸合成法開発

    加瀬光希弥、稲垣雅仁、平岡陽花、橋谷文貴、阿部奈保子、木村康明、阿部洋

    日本化学会CSJ化学フェスタ 

    　More details

    Event date： 2022.10

    Presentation type：Poster presentation  

  20. mRNAの化学修飾位置の特定技術の開発

    大橋咲南、橋谷文貴、阿部洋

    「細胞を創る」研究会15.0 

    　More details

    Event date： 2022.10

    Presentation type：Poster presentation  

  21. 長鎖DNAの細胞内ビルドアップを志向したポスト切断PCRプライマー

    平岡陽花、村瀬裕貴、阿部奈保子、吉田祐希、橋谷文貴、阿部洋

    「細胞を創る」研究会15.0 

    　More details

    Event date： 2022.10

    Presentation type：Poster presentation  

  22. 3´修飾核酸を用いた新規アセンブリ技術開発

    加瀬光希弥、稲垣雅仁、平岡陽花、橋谷文貴、阿部奈保子、木村康明、阿部洋

    学際統合物質機構 若手の会 

    　More details

    Event date： 2022.10

    Presentation type：Poster presentation  

  23. mRNAの化学修飾位置の特定技術の開発

    大橋咲南、橋谷文貴、阿部洋

    学際統合物質機構 若手の会 

    　More details

    Event date： 2022.10

    Presentation type：Poster presentation  

  24. メッセンジャーRNAの完全化学合成と構造活性相関研究

    MENG Zheyu、阿部奈保子、稲垣雅人、LI Zhenmin中嶋裕子、橋谷文貴 、木村 康明、阿部洋

    第43回生体膜と薬物の相互作用シンポジウム 

    　More details

    Event date： 2022.10

    Presentation type：Oral presentation (general)  

  25. ポリメラーゼの基質として機能する四リン酸核酸誘導体の合成および評価

    中村真由、稲垣雅仁、橋谷文貴、木村康明、阿部洋

    第16回バイオ関連化学シンポジウム 

    　More details

    Event date： 2022.9

    Presentation type：Poster presentation  

  26. 化学修飾プライマーを用いた新規DNA連結法とライブラリー構築

    野村浩平・恩田馨・鈴木大輔・村瀬裕貴・稲垣雅仁・平岡陽花・阿部奈保子・橋谷文貴・木村康明・阿部洋

    第16回バイオ関連化学シンポジウム 

    　More details

    Event date： 2022.9

    Presentation type：Poster presentation  

  27. フルオロリン酸アミデート基を用いた新規リン酸プロドラッグの開発

    吉田 祐希・Ti Zheng・田辺 航・友池 史明・橋谷 文貴・廣田 嵩人・齋木 由利子・堀井 明・木村 康明・阿部 洋

    第16回バイオ関連化学シンポジウム 

    　More details

    Event date： 2022.9

    Presentation type：Poster presentation  

  28. 化学的キャップ化反応を用いたmRNAの完全化学合成法の開発

    木村康明・阿部奈保子・今枝昭裕・稲垣雅仁・橋谷文貴・内田智士・岩井宏徒・本間正一・山本潤一郎・阿部洋

    第16回バイオ関連化学シンポジウム 

    　More details

    Event date： 2022.9

    Presentation type：Oral presentation (general)  

  29. mRNAの化学修飾位置の特定技術の開発

    大橋咲南、橋谷文貴、阿部洋

    第16回バイオ関連化学シンポジウム 

    　More details

    Event date： 2022.9

    Presentation type：Poster presentation  

  30. 3’チオ化オリゴ核酸を用いた長鎖DNAアセンブリ技術の開発

    加瀬光希弥、稲垣雅仁、平岡陽花、橋谷文貴、木村康明、阿部洋

    第16回バイオ関連化学シンポジウム 

    　More details

    Event date： 2022.9

    Presentation type：Poster presentation  

  31. 化学的キャップ化反応を用いたmRNAの完全化学合成法の開発

    木村康明，阿部奈保子，今枝昭裕， 橋谷文貴， 内田智士， 岩井宏徒, 　本間正一，山本潤一郎，阿部洋

    日本核酸医薬学会第7回年会 

    　More details

    Event date： 2022.7 - 2022.8

    Presentation type：Poster presentation  

  32. 化学修飾プライマーを用いた長鎖DNA連結技術とライブラリーの構築法

    野村浩平、恩田馨、鈴木大輔、村瀬裕貴、稲垣雅仁、平岡陽花、阿部奈保子、橋谷文貴、木村康明、阿部洋

    日本核酸医薬学会第7回年会 

    　More details

    Event date： 2022.7 - 2022.8

    Presentation type：Poster presentation  

  33. mRNAの化学修飾位置の特定技術の開発

    大橋咲南、橋谷文貴、阿部洋

    日本ケミカルバイオロジー学会 第16回年会 

    　More details

    Event date： 2022.5 - 2022.6

    Presentation type：Poster presentation  

  34. 化学修飾プライマーを用いた長鎖DNA連結技術の開発

    野村浩平、恩田馨、鈴木大輔、Gao Yiuno、村瀬裕貴、中本航介、稲垣雅仁、平岡陽花、阿部奈保子、橋谷文貴、木村康明、阿部洋

    日本ケミカルバイオロジー学会 第16回年会 

    　More details

    Event date： 2022.5 - 2022.6

    Presentation type：Poster presentation  

  35. 貴金属ナノ粒子によるオリゴ核酸鎖切断反応と長鎖オリゴ核酸合成

    稲垣 雅仁、平岡 陽花、加瀬 光希弥、橋谷 文貴、阿部 奈保子、木村 康明、阿部 洋

    日本薬学会第142年会  2022.3.25  日本薬学会

    　More details

    Event date： 2022.3

    Language：English   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  36. 化学修飾プライマーを用いたゲノムスケールDNA合成技術の開発

    野村浩平・恩田馨・Gao Yiuno・村瀬裕貴・中本航介・稲垣雅仁・平岡陽花・阿部奈保子・橋谷文貴・木村康明・阿部洋

    日本薬学会第142年会  2022.3.25  日本薬学会

    　More details

    Event date： 2022.3

    Presentation type：Oral presentation (general)  

    Venue：オンライン  

  37. 化学キャップ化反応を用いたmRNA化学合成法の開発

    "阿部 奈保子、今枝 昭裕、木村 康明、橋谷 文貴、内田 智士、阿部 洋 "

    日本薬学会第142年会  2022.3.25  日本薬学会

    　More details

    Event date： 2022.3

    Presentation type：Oral presentation (general)  

    Venue：オンライン  

  38. 高精度かつ高効率なDNA連結を実現する光保護化学修飾プライマー

    橋谷 文貴・恩田 馨・野村 浩平・Gao Yiuno・村瀬 裕貴・中本 航介・稲垣 雅仁・平岡 陽花・阿部 奈保子・木村 康明・岡 夏央・寺井 悟朗・浅井 潔・阿部 洋

    日本化学会第102春季年会  2022.3.24  日本化学会

    　More details

    Event date： 2022.3

    Language：English   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  39. 新規２′修飾核酸によるｓｉＲＮＡの標的特異性の向上

    馬場 麟太郎・野村浩平・阿部 奈保子・安 成鎮・小林 芳明・程 久美子・橋谷 文貴・木村 康明・阿部 洋

    日本化学会第102春季年会  日本化学会

    　More details

    Event date： 2022.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  40. フルオロリン酸アミデート基を用いた新規リン酸プロドラッグの開発

    吉田 祐希・Ti Zheng・田辺 航・友池 史明・橋谷 文貴・廣田 嵩人・齋木 由利子・堀井 明・木村 康明・阿部 洋

    日本化学会第102春季年会  日本化学会

    　More details

    Event date： 2022.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン  

  41. Chemically modified PCR primer aiming accurate and efficient DNA assembly

    Fumitaka Hashiya, Kaoru Onda, Kohei Nomura, Gao Yiuno, Hirotaka Murase, Kosuke Nakamoto, Masahito Inagaki, Haruka Hiraoka, Naoko Abe, Yasuaki Kimura, Natsuhisa Oka, Goro Terai, Kiyoshi Asai, Hiroshi Abe

    2021.11.4 

    　More details

    Event date： 2021.11

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  42. ホスホロチオエート修飾mRNAの合成とその翻訳活性

    竹渕慧、川口大輔、児玉亜有美、阿部奈保子、橋谷文貴、友池史明、中本航介、木村康明、清水義宏、阿部洋

    第52回 中部化学関係学協会支部連合秋季大会（静岡） 

    　More details

    Event date： 2021.10

    Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  43. メッセンジャーRNAのチオ修飾による翻訳開始反応の促進

    阿部奈保子・川口大輔・児玉亜有実・橋谷文貴・友池史明・木村康明・清水義宏・阿部洋

    第15回バイオ関連化学シンポジウム 

    　More details

    Event date： 2021.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：オンライン   Country：Japan  

  44. 化学的手法を用いた新規ゲノムスケールDNA合成技術の開発

    野村浩平・恩田馨・Gao Yiuno・村瀬裕貴・中本航介・稲垣雅仁・平岡陽花・阿部奈保子・橋谷文貴・木村康明・阿部洋

    第15回バイオ関連化学シンポジウム 

    　More details

    Event date： 2021.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：オンライン   Country：Japan  

  45. フルオロリン酸アミデート基の開発と核酸アナログのプロドラッグへの応用

    木村康明, 吉田祐希, 友池史明,, 橋谷文貴, 鈴木哲朗, 廣田嵩人, 齋木由利子, 堀井明, 平山明由, 曽我朋義, 阿部洋

    第15回バイオ関連化学シンポジウム 

    　More details

    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  46. Simple chemical synthesis of adenylated RNA, a substrate for template independent RNA ligation

    　More details

    Event date： 2021.7

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  47. 化学修飾プライマーを活用した高効率で精密な長鎖DNAアセンブリ―法の開発

    橋谷文貴・恩田馨・野村浩平・GaoYiuno・村瀬裕貴・中本航介・稲垣雅仁・平岡陽花・阿部奈保子・木村康明・阿部洋

    日本核酸医薬学会第6年会  2021.6.27 

    　More details

    Event date： 2021.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  48. 化学修飾プライマーを活用した高効率で精密な長鎖DNAアセンブリ―法の開発

    橋谷文貴・恩田馨・野村浩平・GaoYiuno・村瀬裕貴・中本航介・稲垣雅仁・平岡陽花・阿部奈保子・木村康明・阿部洋

    第15回ケミカルバイオロジー学会  2021.6.21 

    　More details

    Event date： 2021.6

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：オンライン   Country：Japan  

  49. ホスホロチオエート修飾mRNAの合成とその翻訳活性

    竹渕慧、川口大輔、児玉亜有美、阿部奈保子、橋谷文貴、友池史明、中本航介、木村康明、清水義宏、阿部洋

    第15回ケミカルバイオロジー学会 

    　More details

    Event date： 2021.6

    Language：Japanese   Presentation type：Poster presentation  

    Venue：オンライン   Country：Japan  

  50. フルオロリン酸アミデート基を用いた新規リン酸プロドラッグの開発

    吉田祐希・Zheng Ti・田辺航・友池史明・橋谷文貴・鈴木哲朗・廣田嵩人・齋木由利子・堀井明・平山明由・曽我朋義・木村康明・阿部洋

    第15回ケミカルバイオロジー学会 

    　More details

    Event date： 2021.6

    Language：Japanese   Presentation type：Poster presentation  

    Venue：オンライン   Country：Japan  

▼display all

To the head of Presentations.▲

KAKENHI (Grants-in-Aid for Scientific Research) 1

  1. Novel chromatin dynamics analysis exploiting double strand cleavage on DNA

    Grant number：21K14751  2021.4 - 2024.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    　 More details

    Authorship：Principal investigator 

    Grant amount：\4680000 （ Direct Cost: \3600000 、 Indirect Cost：\1080000 ）

To the head of KAKENHI (Grants-in-Aid for Scientific Research).▲

Industrial property rights 4

  1. ポリヌクレオチド連結産物の製造方法

    阿部洋、木村泰明、橋谷文貴、阿部奈保子

    　More details

    Applicant：東海国立大学機構

    Application no：2023-033032  Date applied：2023.3

  2. ポリヌクレオチド連結産物の製造方法

    阿部洋、木村泰明、橋谷文貴、阿部奈保子

    　More details

    Applicant：東海国立大学機構

    Application no：2023-033032  Date applied：2023.3

  3. ポリヌクレオチド連結産物の製造方法

    阿部洋、木村泰明、橋谷文貴、阿部奈保子

    　More details

    Applicant：東海国立大学機構

    Application no：2023-033032  Date applied：2023.3

  4. 認知機能及び手指運動機能の評価訓練用の電子ペグシステム

    岡橋さやか、橋谷文貴、井出慎吾、菅野明弘、内海潤、草場哲、山口太郎

    　More details

    Applicant：一般社団法人芝蘭会

    Application no：特願2019-83343(P2019-83343)  Date applied：2019.4

    Announcement no：特開2020-178851(P2020-178851A)  Date announced：2020.11

To the head of Industrial property rights.▲
 

Teaching Experience (On-campus) 2

  1. 基礎セミナーA

    2021

  2. 基礎セミナーA

    2020

To the head of Teaching Experience (On-campus).▲