Updated on 2022/05/12

写真a

 
HASHIYA Fumitaka
 
Organization
Research Center for Materials Science Assistant Professor
Graduate School
Graduate School of Science
Title
Assistant Professor

Degree 1

  1. PhD ( 2019.3   Kyoto University ) 

Research Interests 4

  1. nucleosome

  2. 生化学

  3. 核酸化学

  4. ケミカルバイオロジー

Research Areas 1

  1. Life Science / Molecular biology

Research History 2

  1. Nagoya University   Assistant Professor

    2019.7

  2. Nagoya University   Researcher

    2019.4 - 2019.7

Education 3

  1. Kyoto University

    2016.4 - 2019.3

  2. Kyoto University

    2014.4 - 2016.3

  3. Kyoto University

    2010.4 - 2014.3

Professional Memberships 4

  1. 日本光生物・光医学会

  2. 日本核酸学会

  3. 日本ケミカルバイオロジー学会

  4. 日本化学会

Awards 2

  1. 奨励賞

    2021.1   日本光医学・光生物学会   5-ブロモウラシルと光反応を利用した新規ヌクレオソーム形成部位の特定手法

    橋谷文貴、杉山弘、阿部洋

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  2. ISNAC Outstanding Oral Presentation Award for Young Scientist in 2021 (Ohtsuka Award)

    2021.11   ISNAC   Chemically modified PCR primer aiming accurate and efficient DNA assembly

 

Papers 18

  1. Development of Fluorophosphoramidate as a Biocompatibly Transformable Functional Group and its Application as a Phosphate Prodrug for Nucleoside Analogs.

    Yoshida Y, Zheng T, Tanabe W, Tomoike F, Hashiya F, Suzuki T, Hirota S, Saiki Y, Horii A, Hirayama A, Soga T, Kimura Y, Abe H

    ChemMedChem     page: e202200188   2022.4

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    Language:English  

    DOI: 10.1002/cmdc.202200188

    PubMed

  2. Antisense Oligonucleotide Modified with Disulfide Units Induces Efficient Exon Skipping in mdx Myotubes through Enhanced Membrane Permeability and Nucleus Internalization

    Hiraoka Haruka, Shu Zhaoma, Tri Le Bao, Masuda Keiko, Nakamoto Kosuke, Fangjie Lyu, Abe Naoko, Hashiya Fumitaka, Kimura Yasuaki, Shimizu Yoshihiro, Veedu Rakesh N., Abe Hiroshi

    CHEMBIOCHEM   Vol. 22 ( 24 ) page: 3437 - 3442   2021.12

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    Language:Japanese   Publisher:ChemBioChem  

    We have found that antisense oligonucleotides and siRNA molecules modified with repeat structures of disulfide units can be directly introduced into the cytoplasm and exhibit a suppressive effect on gene expression. In this study, we analyzed the mechanism of cellular uptake of these membrane-permeable oligonucleotides (MPONs). Time-course analysis by confocal microscopy showed that the uptake of MPONs from the plasma membrane to the cytoplasm reached 50 % of the total uptake in about 5 min. In addition, analysis of the plasma membrane proteins to which MPONs bind, identified several proteins, including voltage-dependent anion channel. Next, we analyzed the behavior of MPONs in the cell and found them to be abundant in the nucleus as early as 24 h after addition with the amount increasing further after 48 and 72 h. The amount of MPONs was 2.5-fold higher than that of unmodified oligonucleotides in the nucleus after 72 h. We also designed antisense oligonucleotides and evaluated the effect of MPONs on mRNA exon skipping using DMD model cells; MPONs caused exon skipping with 69 % efficiency after 72 h, which was three times higher than the rate of the control. In summary, the high capacity for intracytoplasmic and nuclear translocation of MPONs is expected to be useful for therapeutic strategies targeting exon skipping.

    DOI: 10.1002/cbic.202100413

    Web of Science

    Scopus

    PubMed

  3. Complete chemical synthesis of long DNA transcriptable in human cells

    Kazuki Yamaoka, Ryota Oikawa, Naoko Abe, Kosuke Nakamoto, Fumiaki Tomoike, Fumitaka Hashiya, Yasuaki Kimura, Hiroshi Abe

    ChemBioChem   Vol. 22 ( 23 ) page: 3273 - 3276   2021.12

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/cbic.202100312

    Web of Science

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  4. Variety of nucleotide polymerase mutants aiming to synthesize modified RNA

    Sana Ohashi, Fumitaka Hashiya, Hiroshi Abe

    ChemBioChem   Vol. 22 ( 14 ) page: 2398 - 2406   2021.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/cbic.202100004

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  5. A novel method for locating nucleosomes by exploiting 5-bromouracil, pyrene-modified histone, and photoirradiation Reviewed

    Fumitaka Hashiya, Hiroshi Sugiyama

    Photomedicine and Photobiology   Vol. 42   page: 13 - 16   2021

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  6. A novel method for locating nucleosomes by exploiting 5-Bromouracil. pyrene-modified histone, and photoirradiation Reviewed

    Photomedicine and photobiology   Vol. 42   page: 13 - 16   2021

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  7. Direct Observation of Dynamic Interactions between Orientation-Controlled Nucleosomes in a DNA Origami Frame. International journal

    Yihong Feng, Fumitaka Hashiya, Kumi Hidaka, Hiroshi Sugiyama, Masayuki Endo

    Chemistry (Weinheim an der Bergstrasse, Germany)   Vol. 26 ( 66 ) page: 15282 - 15289   2020.11

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    The nucleosome is one of the most fundamental units involved in gene expression and consequent cell development, differentiation, and expression of cell functions. We report here a method to place reconstituted nucleosomes into a DNA origami frame for direct observation using high-speed atomic-force microscopy (HS-AFM). By using this method, multiple nucleosomes can be incorporated into a DNA origami frame and real-time movement of nucleosomes can be visualized. The arrangement and conformation of nucleosomes and the distance between two nucleosomes can be designed and controlled. In addition, four nucleosomes can be placed in a DNA frame. Multiple nucleosomes were well accessible in each conformation. Dynamic movement of the individual nucleosomes were precisely monitored in the DNA frame, and their assembly and interaction were directly observed. Neither mica surface modification nor chemical fixation of nucleosomes is used in this method, meaning that the DNA frame not only holds nucleosomes, but also retains their natural state. This method offers a promising platform for investigating nucleosome interactions and for studying chromatin structure.

    DOI: 10.1002/chem.202003071

    Web of Science

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  8. Phosphorothioate Modification of mRNA Accelerates the Rate of Translation Initiation to Provide More Efficient Protein Synthesis. International journal

    Daisuke Kawaguchi, Ayumi Kodama, Naoko Abe, Kei Takebuchi, Fumitaka Hashiya, Fumiaki Tomoike, Kosuke Nakamoto, Yasuaki Kimura, Yoshihiro Shimizu, Hiroshi Abe

    Angewandte Chemie (International ed. in English)   Vol. 59 ( 40 ) page: 17403 - 17407   2020.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Messenger RNAs (mRNAs) with phosphorothioate modification (PS-mRNA) to the phosphate site of A, G, C, and U with all 16 possible combinations were prepared, and the translation reaction was evaluated using an E. coli cell-free translation system. Protein synthesis from PS-mRNA increased in 12 of 15 patterns when compared with that of unmodified mRNA. The protein yield increased 22-fold when the phosphorothioate modification at A/C sites was introduced into the region from the 5'-end to the initiation codon. Single-turnover analysis of PS-mRNA translation showed that phosphorothioate modification increases the number of translating ribosomes, thus suggesting that the rate of translation initiation (rate of ribosome complex formation) is positively affected by the modification. The method provides a new strategy for improving translation by using non-natural mRNA.

    DOI: 10.1002/anie.202007111

    Web of Science

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  9. A synthetic transcription factor pair mimic for precise recruitment of an epigenetic modifier to the targeted DNA locus. International journal

    Zutao Yu, Mengting Ai, Soumen K Samanta, Fumitaka Hashiya, Junichi Taniguchi, Sefan Asamitsu, Shuji Ikeda, Kaori Hashiya, Toshikazu Bando, Ganesh N Pandian, Lyle Isaacs, Hiroshi Sugiyama

    Chemical communications (Cambridge, England)   Vol. 56 ( 15 ) page: 2296 - 2299   2020.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    We developed an epigenetically active, cooperative DNA binding transcription factor platform assisted by cucurbit[7]uril (CB7) host-guest modules. This new type of molecule termed ePIP-HoGu not only mimics the operation of transcription factors as a pair but also recruits the epigenetic modifier to a particular DNA locus.

    DOI: 10.1039/c9cc09608f

    PubMed

  10. Translational control by secondary-structure formation in mRNA in a eukaryotic system. International journal

    Daisuke Kawaguchi, Saaya Shimizu, Naoko Abe, Fumitaka Hashiya, Fumiaki Tomoike, Yasuaki Kimura, Hiroshi Abe

    Nucleosides, nucleotides & nucleic acids   Vol. 39 ( 1-3 ) page: 195 - 203   2020.2

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Eukaryotic mRNA has a cap structure at the 5' end and a poly(A) tail at the 3' end. The cap and poly(A) tail form a complex with multiple translation factors, and mRNA forms a circularized structure called a closed-loop model. This circularized structure reportedly not only stabilizes mRNA but also promotes ribosome recycling during translation, which improves translation efficiency. We designed an artificial mRNA that forms a circularized structure without a cap structure and poly(A) tail and found that its translational efficiency was improved compared with that of a sequence without the circularized structure in a eukaryotic translation system.

    DOI: 10.1080/15257770.2019.1671593

    Web of Science

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  11. Electron injection from mitochondrial transcription factor A to DNA associated with thymine dimer photo repair. International journal

    Fumitaka Hashiya, Shinji Ito, Hiroshi Sugiyama

    Bioorganic & medicinal chemistry   Vol. 27 ( 2 ) page: 278 - 284   2019.1

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    Authorship:Lead author   Publishing type:Research paper (scientific journal)  

    Electron transfer through π-stacked arrays of double-stranded DNA contributes to the redox chemistry of bases, including guanine oxidation and thymine-thymine dimer repair by photolyase. 5-Bromouracil is an attractive photoreactive thymine analogue that can be used to investigate electron transfer in DNA, and is a useful probe for protein-DNA interaction analysis. In the present study using BrU we found that UV irradiation facilitated electron injection from mitochondrial transcription factor A into DNA. We also observed that this electron injection could lead to repair of a thymine-thymine dimer.

    DOI: 10.1016/j.bmc.2018.11.044

    PubMed

  12. Approach to the Investigation of Nucleosome Structure by Using the Highly Emissive Nucleobase th dG-tC FRET Pair. International journal

    Ji Hoon Han, Soyoung Park, Fumitaka Hashiya, Hiroshi Sugiyama

    Chemistry (Weinheim an der Bergstrasse, Germany)   Vol. 24 ( 64 ) page: 17091 - 17095   2018.11

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    Publishing type:Research paper (scientific journal)  

    A distance- and orientation-factor-dependent FRET system is a useful and attractive approach to the investigation of the conformational dynamics of nucleosomes. In this study, the application of the highly emissive nucleobase th dG-tC FRET pair to 601 nucleosomes is reported. It was found that the th dG-tC FRET pair was successfully incorporated to 145 bp 601 sequences, and different FRET efficiencies were obtained for the designated donor and acceptor positions in the nucleosome.

    DOI: 10.1002/chem.201803382

    PubMed

  13. Direct Observation of H3-H4 Octasome by High-Speed AFM. International journal

    Tingting Zou, Fumitaka Hashiya, Yulei Wei, Zutao Yu, Ganesh N Pandian, Hiroshi Sugiyama

    Chemistry (Weinheim an der Bergstrasse, Germany)   Vol. 24 ( 60 ) page: 15998 - 16002   2018.10

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    Publishing type:Research paper (scientific journal)  

    Despite evidence that histone H3 and H4 proteins may act as the precursor for orientating the DNA sequence to form nucleosome structures, there is no direct evidence of the formed compact structure. Here, it is demonstrated that a histone H3-H4 octasome could be constructed without the involvement of histone H2A-H2B under in vitro reconstitution conditions. Atomic force microscopy was used to obtain the first direct observation of the octasome structure, which exhibited a similar core-protein size as that of a nucleosome but with a shorter core histone-binding DNA region. The octasome also displayed a one-step histone-dissociation pattern under heat treatment, distinct micrococcal nuclease and peplomycin accessibility, which suggests a different wrapping pattern to that in nucleosomes.

    DOI: 10.1002/chem.201804010

    PubMed

  14. Biomimetic Artificial Epigenetic Code for Targeted Acetylation of Histones. International journal

    Junichi Taniguchi, Yihong Feng, Ganesh N Pandian, Fumitaka Hashiya, Takuya Hidaka, Kaori Hashiya, Soyoung Park, Toshikazu Bando, Shinji Ito, Hiroshi Sugiyama

    Journal of the American Chemical Society   Vol. 140 ( 23 ) page: 7108 - 7115   2018.6

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    Publishing type:Research paper (scientific journal)  

    While the central role of locus-specific acetylation of histone proteins in eukaryotic gene expression is well established, the availability of designer tools to regulate acetylation at particular nucleosome sites remains limited. Here, we develop a unique strategy to introduce acetylation by constructing a bifunctional molecule designated Bi-PIP. Bi-PIP has a P300/CBP-selective bromodomain inhibitor (Bi) as a P300/CBP recruiter and a pyrrole-imidazole polyamide (PIP) as a sequence-selective DNA binder. Biochemical assays verified that Bi-PIPs recruit P300 to the nucleosomes having their target DNA sequences and extensively accelerate acetylation. Bi-PIPs also activated transcription of genes that have corresponding cognate DNA sequences inside living cells. Our results demonstrate that Bi-PIPs could act as a synthetic programmable histone code of acetylation, which emulates the bromodomain-mediated natural propagation system of histone acetylation to activate gene expression in a sequence-selective manner.

    DOI: 10.1021/jacs.8b01518

    PubMed

  15. A novel detection technique of polyamide binding sites by photo-induced electron transfer in (Br)U substituted DNA. Invited Reviewed

    Chemical Communications   Vol. 51 ( 77 ) page: 14485 - 14488   2015.10

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    Language:English  

  16. Locating the uracil-5-yl radical formed upon photoirradiation of 5-bromouracil-substituted DNA. Invited Reviewed

    Nucleic acids research   Vol. 42 ( 22 ) page: 13469 - 13473   2014.12

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    Language:English  

  17. Sequence-specific DNA alkylation and transcriptional inhibition by long-chain hairpin pyrrole-imidazole polyamide-chlorambucil conjugates targeting CAG/CTG trinucleotide repeats. Invited Reviewed

    Bioorganic & medicinal chemistry.   Vol. 22 ( 17 ) page: 4646 - 4657   2014.9

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    Language:English  

  18. Sequence-specific electron injection into DNA from an intermolecular electron donor. Reviewed

    Hironobu Morinaga, Tomohiro Takenaka, Fumitaka Hashiya, Seiichiro Kizaki, Kaori Hashiya, Toshikazu Bando, Hiroshi Sugiyama.

    Nucleic acids research   Vol. 41 ( 8 ) page: 4724 - 4728   2013.4

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    Language:English   Publishing type:Research paper (scientific journal)  

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Presentations 18

  1. 貴金属ナノ粒子によるオリゴ核酸鎖切断反応と長鎖オリゴ核酸合成

    稲垣 雅仁、平岡 陽花、加瀬 光希弥、橋谷 文貴、阿部 奈保子、木村 康明、阿部 洋

    日本薬学会第142年会  2022.3.25  日本薬学会

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    Event date: 2022.3

    Language:English   Presentation type:Oral presentation (general)  

    Venue:オンライン  

  2. 化学修飾プライマーを用いたゲノムスケールDNA合成技術の開発

    野村浩平・恩田馨・Gao Yiuno・村瀬裕貴・中本航介・稲垣雅仁・平岡陽花・阿部奈保子・橋谷文貴・木村康明・阿部洋

    日本薬学会第142年会  2022.3.25  日本薬学会

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    Event date: 2022.3

    Venue:オンライン  

  3. 化学キャップ化反応を用いたmRNA化学合成法の開発

    "阿部 奈保子、今枝 昭裕、木村 康明、橋谷 文貴、内田 智士、阿部 洋 "

    日本薬学会第142年会  2022.3.25  日本薬学会

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    Event date: 2022.3

    Venue:オンライン  

  4. 高精度かつ高効率なDNA連結を実現する光保護化学修飾プライマー

    橋谷 文貴・恩田 馨・野村 浩平・Gao Yiuno・村瀬 裕貴・中本 航介・稲垣 雅仁・平岡 陽花・阿部 奈保子・木村 康明・岡 夏央・寺井 悟朗・浅井 潔・阿部 洋

    日本化学会第102春季年会  2022.3.24  日本化学会

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    Event date: 2022.3

    Language:English   Presentation type:Oral presentation (general)  

    Venue:オンライン  

  5. 新規2′修飾核酸によるsiRNAの標的特異性の向上

    馬場 麟太郎・野村浩平・阿部 奈保子・安 成鎮・小林 芳明・程 久美子・橋谷 文貴・木村 康明・阿部 洋

    日本化学会第102春季年会  日本化学会

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    Event date: 2022.3

    Venue:オンライン  

  6. フルオロリン酸アミデート基を用いた新規リン酸プロドラッグの開発

    吉田 祐希・Ti Zheng・田辺 航・友池 史明・橋谷 文貴・廣田 嵩人・齋木 由利子・堀井 明・木村 康明・阿部 洋

    日本化学会第102春季年会  日本化学会

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    Event date: 2022.3

    Venue:オンライン  

  7. Chemically modified PCR primer aiming accurate and efficient DNA assembly

    Fumitaka Hashiya, Kaoru Onda, Kohei Nomura, Gao Yiuno, Hirotaka Murase, Kosuke Nakamoto, Masahito Inagaki, Haruka Hiraoka, Naoko Abe, Yasuaki Kimura, Natsuhisa Oka, Goro Terai, Kiyoshi Asai, Hiroshi Abe

    2021.11.11 

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    Event date: 2021.11

    Presentation type:Oral presentation (general)  

    Country:Japan  

  8. Chemically modified PCR primer aiming accurate and efficient DNA assembly

    Fumitaka Hashiya, Kaoru Onda, Kohei Nomura, Gao Yiuno, Hirotaka Murase, Kosuke Nakamoto, Masahito Inagaki, Haruka Hiraoka, Naoko Abe, Yasuaki Kimura, Natsuhisa Oka, Goro Terai, Kiyoshi Asai, Hiroshi Abe

    2021.11.4 

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    Event date: 2021.11

    Presentation type:Oral presentation (general)  

    Country:Japan  

  9. ホスホロチオエート修飾mRNAの合成とその翻訳活性

    竹渕慧、川口大輔、児玉亜有美、阿部奈保子、橋谷文貴、友池史明、中本航介、木村康明、清水義宏、阿部洋

    第52回 中部化学関係学協会支部連合秋季大会(静岡) 

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    Event date: 2021.10

    Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  10. メッセンジャーRNAのチオ修飾による翻訳開始反応の促進

    阿部奈保子・川口大輔・児玉亜有実・橋谷文貴・友池史明・木村康明・清水義宏・阿部洋

    第15回バイオ関連化学シンポジウム 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン   Country:Japan  

  11. 化学的手法を用いた新規ゲノムスケールDNA合成技術の開発

    野村浩平・恩田馨・Gao Yiuno・村瀬裕貴・中本航介・稲垣雅仁・平岡陽花・阿部奈保子・橋谷文貴・木村康明・阿部洋

    第15回バイオ関連化学シンポジウム 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン   Country:Japan  

  12. フルオロリン酸アミデート基の開発と核酸アナログのプロドラッグへの応用

    木村康明, 吉田祐希, 友池史明,, 橋谷文貴, 鈴木哲朗, 廣田嵩人, 齋木由利子, 堀井明, 平山明由, 曽我朋義, 阿部洋

    第15回バイオ関連化学シンポジウム 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  13. Simple chemical synthesis of adenylated RNA, a substrate for template independent RNA ligation

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    Event date: 2021.7

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  14. 化学修飾プライマーを活用した高効率で精密な長鎖DNAアセンブリ―法の開発

    橋谷文貴・恩田馨・野村浩平・GaoYiuno・村瀬裕貴・中本航介・稲垣雅仁・平岡陽花・阿部奈保子・木村康明・阿部洋

    日本核酸医薬学会第6年会  2021.6.27 

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    Event date: 2021.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  15. 化学修飾プライマーを活用した高効率で精密な長鎖DNAアセンブリ―法の開発

    橋谷文貴・恩田馨・野村浩平・GaoYiuno・村瀬裕貴・中本航介・稲垣雅仁・平岡陽花・阿部奈保子・木村康明・阿部洋

    第15回ケミカルバイオロジー学会  2021.6.21 

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    Event date: 2021.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  16. ホスホロチオエート修飾mRNAの合成とその翻訳活性

    竹渕慧、川口大輔、児玉亜有美、阿部奈保子、橋谷文貴、友池史明、中本航介、木村康明、清水義宏、阿部洋

    第15回ケミカルバイオロジー学会 

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    Event date: 2021.6

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン   Country:Japan  

  17. フルオロリン酸アミデート基を用いた新規リン酸プロドラッグの開発

    吉田祐希・Zheng Ti・田辺航・友池史明・橋谷文貴・鈴木哲朗・廣田嵩人・齋木由利子・堀井明・平山明由・曽我朋義・木村康明・阿部洋

    第15回ケミカルバイオロジー学会 

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    Event date: 2021.6

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン   Country:Japan  

  18. 5-ブロモウラシルと光反応を利用した新規ヌクレオソーム形成部位の特定手法 Invited

    橋谷文貴、杉山弘、阿部洋

    第42回日本光医学・光生物学会  2021.1.22 

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    Event date: 2021.1

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

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KAKENHI (Grants-in-Aid for Scientific Research) 1

  1. Novel chromatin dynamics analysis exploiting double strand cleavage on DNA

    Grant number:21K14751  2021.4 - 2024.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

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    Authorship:Principal investigator 

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

Industrial property rights 1

  1. 認知機能及び手指運動機能の評価訓練用の電子ペグシステム

    岡橋さやか、橋谷文貴、井出慎吾、菅野明弘、内海潤、草場哲、山口太郎

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    Applicant:一般社団法人芝蘭会

    Application no:特願2019-83343(P2019-83343)  Date applied:2019.4

    Announcement no:特開2020-178851(P2020-178851A)  Date announced:2020.11

 

Teaching Experience (On-campus) 2

  1. 基礎セミナーA

    2021

  2. 基礎セミナーA

    2020