Updated on 2021/10/20

写真a

 
MIZUTA Yoko
 
Organization
Institute for Advanced Research Designated assistant professor
Institute of Transformative Bio-Molecules Designated assistant professor
Title
Designated assistant professor

Degree 1

  1. 博士(理学) ( 2010.3   総合研究大学院大学 ) 

Research Interests 8

  1. 植物生殖

  2. イメージング

  3. 花粉管

  4. ゲノム編集

  5. 二光子励起顕微鏡

  6. 生殖的隔離

  7. 植物分子生物学

  8. 花粉

Research Areas 4

  1. Life Science / Morphology and anatomical structure

  2. Life Science / Plant molecular biology and physiology

  3. Environmental Science/Agriculture Science / Science in plant genetics and breeding

  4. Life Science / Cell biology

Current Research Project and SDGs 3

  1. イメージングを基盤とした植物の生殖機構の解明

  2. 花粉による新植物育種技術の開発

  3. 花粉の発生と受精能を決定する分子メカニズムの解明

Research History 5

  1. Nagoya University

    2019.4

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    Country:Japan

  2. Japan Science and Technology Agency

    2016.4 - 2019.3

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    Country:Japan

  3. 名古屋大学大学院   理学研究科 ERATO東山ライブホロニクスプロジェクト   研究員

    2011.4 - 2016.3

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    Country:Japan

  4. 情報・システム研究機構 国立遺伝学研究所   植物遺伝研究室   特任研究員

    2010.4 - 2011.3

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    Country:Japan

  5. 日本学術振興会特別研究員DC1

    2007.4 - 2010.3

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    Country:Japan

Education 2

  1. The Graduate University for Advanced Studies

    2005.4 - 2010.3

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    Country: Japan

  2. Niigata University   Faculty of Agriculture

    2001.4 - 2005.3

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    Country: Japan

Professional Memberships 3

  1. THE JAPANESE SOCIETY OF PLANT MORPHOLOGY

  2. THE BOTANICAL SOCIETY OF JAPAN

  3. THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS

Committee Memberships 2

  1. Frontiers in Genome editing   Review Editor, Editorial Board of Genome Editing in Plants  

    2020.7   

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    Committee type:Academic society

  2. 文部科学省 科学技術・学術政策研究所 科学技術予測センター(NISTEP)   専門調査員  

    2020.4 - 2021.3   

Awards 9

  1. NIKON JOICO AWARD 2019 優秀賞

    2020.1   株式会社ニコンインステック   花のなかの秘密

    水多陽子

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    Country:Japan

  2. 中手賞2018

    2018.12   農学中手の会 第4回研究集会   花粉管をベクターとして植物を変える

    水多 陽子

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

    http://www.nakate.website

  3. 第11回 科学技術の「美」パネル展 優秀賞

    2017.4   科学技術団体連合   「めしべを丸ごと透明化」

    水多 陽子

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    Award type:Award from publisher, newspaper, foundation, etc.  Country:Japan

    https://www.jst.go.jp/report/2017/170418.html

  4. 平瀬賞

    2016.9   日本植物形態学会   ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging. Development 142: 4168-4179

    D. Kurihara, Y. Mizuta, Y. Sato and T. Higashiyama.

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    Award type:Honored in official journal of a scientific society, scientific journal  Country:Japan

    http://square.umin.ac.jp/pl-morph/pdf/170810_Hirase_papers.pdf

  5. Cell Picture Show (顕微鏡写真コンテスト、ZEISS社 2016年 国際カレンダー採用)

    2016   Cell Press and Zeiss   Where the Magic Happens

    Yoko Mizuta, Daisuke Kurihara Tetsuya Higashiyama

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    Country:Germany

    https://www.cell.com/pictureshow/biology-of-sex

  6. 日本植物学会第79回大会 ロゴマーク採用

    2014.11   日本植物学会第79回大会   ユキツバキ

    水多陽子

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

    http://bsj.or.jp/jpn/members/information/622.php

  7. 最優秀ポスター賞

    2014.1   新学術領域研究 動植物に共通するアロ認証機構の解明 第8回領域会議   2光子顕微鏡を用いた花粉管の受精競争と多精拒否のin vivoライブイメージング

    水多陽子

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  8. 優秀発表賞

    2010.3   日本育種学会第117回講演会   イネ亜種間交雑で生殖的隔離を引き起こすDOPPELGANGER(DPL)1とDPL2の解析

    水多 陽子、春島 嘉章、倉田 のり

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

    https://www.nacos.com/jsb/07/07prize_2010.html

  9. Best Paper賞

    2009.9   日本遺伝学会第81回大会   イネ亜種間交雑において生殖的隔離を引き起こす相互作用遺伝子座DOPPELGANGER1と2の単離・解析

    水多陽子、春島嘉章、倉田のり

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

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Papers 24

  1. Detection of a biolistic delivery of fluorescent markers and CRISPR/Cas9 to the pollen tube Reviewed

    Shiori Nagahara, Tetsuya Higashiyama, Yoko Mizuta

    Plant Reproduction   Vol. 34 ( 3 ) page: 191 - 205   2021.9

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    Authorship:Last author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    <title>Abstract</title><sec>
    <title>Key message</title>
    <bold><italic>Biolistic delivery into pollen.</italic></bold>


    </sec><sec>
    <title>Abstract</title>
    In recent years, genome editing techniques, such as the CRISPR/Cas9 system, have been highlighted as a new approach to plant breeding. <italic>Agrobacterium</italic>-mediated transformation has been widely utilized to generate transgenic plants by introducing plasmid DNA containing CRISPR/Cas9 into plant cells. However, this method has general limitations, such as the limited host range of <italic>Agrobacterium</italic> and difficulties in tissue culture, including callus induction and regeneration. To avoid these issues, we developed a method to genetically modify germ cells without the need for <italic>Agrobacterium</italic>-mediated transfection and tissue culture using tobacco as a model. In this study, plasmid DNA containing sequences of Cas9, guide RNA, and fluorescent reporter was introduced into pollen using a biolistic delivery system. Based on the transient expression of fluorescent reporters, the <italic>Arabidopsis UBQ10</italic> promoter was found to be the most suitable promoter for driving the expression of the delivered gene in pollen tubes. We also evaluated the delivery efficiency in male germ cells in the pollen by expression of the introduced fluorescent marker. Mutations were detected in the target gene in the genomic DNA extracted from CRISPR/Cas9-introduced pollen tubes, but were not detected in the negative control. Bombarded pollen germinated pollen tubes and delivered their contents into the ovules in vivo. Although it is necessary to improve biolistic delivery efficiency and establish a method for the screening of genome-modified seeds, our findings provide important insights for the detection and production of genome-modified seeds by pollen biolistic delivery.


    </sec>

    DOI: 10.1007/s00497-021-00418-z

    Web of Science

    Scopus

    PubMed

    Other Link: https://link.springer.com/article/10.1007/s00497-021-00418-z/fulltext.html

  2. Advances in Two-Photon Imaging in Plants. Reviewed

    Yoko Mizuta

    Plant & cell physiology     2021.5

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Live and deep imaging play a significant role in the physiological and biological studies of organisms. Two-photon excitation microscopy (2PEM), also known as multiphoton excitation microscopy, is a fluorescent imaging technique that allows deep imaging of living tissues. Two-photon lasers use near-infrared pulse lasers that are less invasive and permit deep tissue penetration. In this review, recent advances in two-photon imaging and their applications in plant studies are discussed. Compared to confocal microscopy, near-infrared 2PEM exhibits reduced plant-specific autofluorescence, thereby achieving greater depth and high-resolution imaging in plant tissues. Fluorescent proteins with long emission wavelengths, such as orange-red fluorescent proteins, are particularly suitable for two-photon live imaging in plants. Furthermore, deep- and high-resolution imaging was achieved using plant-specific clearing methods. In addition to imaging, optical cell manipulations can be performed using femtosecond pulsed lasers at the single cell or organelle level. Optical surgery and manipulation can reveal cellular communication during development. Advances in in vivo imaging using 2PEM will greatly benefit biological studies in plant sciences.

    DOI: 10.1093/pcp/pcab062

    PubMed

  3. ClearSeeAlpha: Advanced Optical Clearing for Whole-Plant Imaging Reviewed

    Daisuke Kurihara, Yoko Mizuta, Shiori Nagahara, Tetsuya Higashiyama

    Plant And Cell Physiology     2021.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    DOI: 10.1093/pcp/pcab033

    PubMed

  4. A Peptide Pair Coordinates Regular Ovule Initiation Patterns with Seed Number and Fruit Size Reviewed

    Nozomi Kawamoto, Dunia Pino Del Carpio, Alexander Hofmann, Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama, Naoyuki Uchida, Keiko U. Torii, Lucia Colombo, Georg Groth, Rüdiger Simon

    Current Biology   Vol. 30 ( 22 ) page: 4352 - +   2020.11

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.cub.2020.08.050

    Web of Science

    Scopus

    PubMed

  5. Technologies for the understanding and control of biological phenomena in field-grown plants Reviewed

    Yamauchi Takaki, Inukai Yoshiaki, Fujii Sota, Okamoto Masanori, Tanaka Yu, Mizuta Yoko, Hiruma Kei, Yoshida Kentaro, Yamamoto Eiji, Ohnishi Takayuki

    Breeding Research   Vol. 22 ( 1 ) page: 75-82   2020

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1270/jsbbr.22.W03

    CiNii Article

  6. Chemical signaling for pollen tube guidance at a glance Invited Reviewed

    Yoko Mizuta, Tetsuya Higashiyama

    Journal of Cell Science   Vol. 131 ( 2 ) page: jcs208447   2018.1

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Company of Biologists Ltd  

    Pollen tube guidance is a unique navigating system that is required for the successful sexual reproduction of plants. As plant sperm cells are non-motile and egg cells are embedded deep inside the female tissues, a pollen tube delivers the two sperm cells that it contains by growing towards the ovule, in which the egg cell resides. Pollen tube growth towards the ovule is precisely controlled and divided into two stages, preovular and ovular guidance. In this Cell Science at a Glance article and accompanying poster, we provide a comprehensive overview of pollen tube guidance and highlight some of the attractant peptides used during ovular guidance. We further discuss the precise one-to-one guidance system that exists in multi-ovular plants. The pollen tube-blocking system, which is mediated by male-female crosstalk communication, to avoid attraction of multiple pollen tubes, is also reviewed.

    DOI: 10.1242/jcs.208447

    Web of Science

    Scopus

    PubMed

  7. Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee. Reviewed

    Yoko Mizuta, Katsutoshi Tsuda

    Methods in molecular biology (Clifton, N.J.)   Vol. 1830   page: 257 - 268   2018

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/978-1-4939-8657-6_15

    Web of Science

    Scopus

    PubMed

  8. Plant tissue clearing for fluorescence imaging Invited

    Daisuke Kurihara, Yoko Mizuta

    Plant Morphology   Vol. 29 ( 1 ) page: 81-86   2017.4

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    Language:Japanese  

  9. Visualization of plant sexual reproduction in the whole-mount pistil by Clearsee Reviewed

    Yoko Mizuta,Daisuke Kurihara, Tetsuya Higashiyama

    Cytologia   Vol. 81   page: 1−2   2016.1

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    Authorship:Lead author, Corresponding author   Language:English  

  10. ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging. Reviewed

    Daisuke Kurihara, Yoko Mizuta, Yoshikatsu Sato, Tetsuya Higashiyama

    Development   Vol. 142 ( 23 ) page: 4168-4179   2015.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1242/dev.127613

    PubMed

  11. Two-photon imaging with longer wavelength excitation in intact Arabidopsis tissues. Reviewed

    Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama

    Protoplasma   Vol. 252 ( 5 ) page: 1231-1240   2015.9

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00709-014-0754-5

    PubMed

  12. In vivo deep imaging and optical manipulation by two-photon excitation microscopy(<Feature Articles>Live-cell imaging) Invited

    Mizuta Yoko, Kurihara Daisuke

    Regulation of Plant Growth & Development   Vol. 49 ( 2 ) page: 96-103   2014.12

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    Authorship:Lead author   Language:Japanese  

  13. Antisense gene inhibition by phosphorothioate antisense oligonucleotide in Arabidopsis pollen tubes. Reviewed

    Yoko Mizuta, Tetsuya Higashiyama

    The Plant journal   Vol. 78 ( 3 ) page: 516-526   2014.5

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/tpj.12461

    PubMed

  14. Growth assay of individual pollen tubes arrayed by microchannel device

    Mitsuhiro Horade, Naoki Yanagisawa, Yoko Mizuta, Tetsuya Higashiyama, Hideyuki Arata

    Microelectronic Engineering   Vol. 118   page: 25-28   2014.4

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  15. 花粉管のin vivoイメージングで観えてきた植物生殖の実態—2光子顕微鏡を用いたアプローチ— Invited Reviewed

    水多陽子, 栗原大輔, 東山哲也

    PLANT MORPHOLOGY   Vol. 26 ( 1 ) page: 71   2014.4

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    Authorship:Lead author   Language:Japanese  

  16. In vivo live-cell imaging in plant tissues by two-photon excitation microscopy Invited

    Mizuta Yoko, Kurihara Daisuke, Higashiyama Tetsuya

    PLANT MORPHOLOGY   Vol. 26 ( 1 ) page: 25-30   2014

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    Authorship:Lead author, Corresponding author   Language:Japanese  

  17. Plant-on-a-chip microfluidic-system for quantitative analysis of pollen tube guidance by signaling molecule: Towards cell-to-cell communication study Reviewed

    Mitsuhiro Horade, Yoko Mizuta, Noritada Kaji, Tetsuya Higashiyama, Hideyuki Arata

    Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012     page: 1027-1029   2012.1

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    Language:English  

  18. Rice expression atlas in reproductive development. Reviewed

    Fujita M, Horiuchi Y, Ueda Y, Mizuta Y, Kubo T, Yano K, Yamaki S, Tsuda K, Nagata T, Niihama M, Kato H, Kikuchi S, Hamada K, Mochizuki T, Ishimizu T, Iwai H, Tsutsumi N, Kurata N

    Plant & cell physiology   Vol. 51 ( 12 ) page: 2060-2081   2010.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcq165

    PubMed

  19. Rice pollen hybrid incompatibility caused by reciprocal gene loss of duplicated genes. Reviewed

    Yoko Mizuta, Yoshiaki Harushima, Nori Kurata

    Proceedings of the National Academy of Sciences of the United States of America   Vol. 107 ( 47 ) page: 20417-20422   2010.11

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1073/pnas.1003124107

    PubMed

  20. Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray. Reviewed

    Suwabe K, Suzuki G, Takahashi H, Shiono K, Endo M, Yano K, Fujita M, Masuko H, Saito H, Fujioka T, Kaneko F, Kazama T, Mizuta Y, Kawagishi-Kobayashi M, Tsutsumi N, Kurata N, Nakazono M, Watanabe M

    Plant & cell physiology   Vol. 49 ( 10 ) page: 1407-1416   2008.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcn124

    PubMed

  21. Various spatiotemporal expression profiles of anther-expressed genes in rice. Reviewed

    Hobo T, Suwabe K, Aya K, Suzuki G, Yano K, Ishimizu T, Fujita M, Kikuchi S, Hamada K, Miyano M, Fujioka T, Kaneko F, Kazama T, Mizuta Y, Takahashi H, Shiono K, Nakazono M, Tsutsumi N, Nagamura Y, Kurata N, Watanabe M, Matsuoka M

    Plant & cell physiology   Vol. 49 ( 10 ) page: 1417-1428   2008.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcn128

    PubMed

  22. Morphological Characterization of Three Intergeneric Hybrids Among Gloriosa superba `Lutea', Littonia modesta, and Sandersonia aurantiaca (Colchicaceae) Reviewed

    Amano J, Kuwayama S, Mizuta Y, Nakano M, Godo T, Okuno H

    Hort. Science   Vol. 43   page: 115-118   2008

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    Language:English   Publishing type:Research paper (scientific journal)  

  23. Mapping of a pair of reproductive barrier loci observed in a cross between Nipponbare and Kasalath

    Yoko Mizuta, Yoshiaki Harushima, Nori Kurata

    Rice Genetics Newsletter   Vol. 23   page: 33-35   2007

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    Authorship:Lead author   Language:English  

  24. Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses

    Amano Junji, Kuwayama Sachiko, Mizuta Yoko, Oomiya Tomo, Nakamura Toru, Nakano Masaru

    Journal of the Japanese Society for Horticultural Science   Vol. 76 ( 1 ) page: 73-78   2007

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    Language:English  

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Books 5

  1. エッセンシャル遺伝学・ゲノム科学 (原著第7版) 第9章 Reviewed

    植田 美那子, 栗原 大輔, 水多 陽子( Role: Joint translator ,  第9章)

    化学同人  2021.2  ( ISBN:9784759820485

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    Language:Japanese Book type:Textbook, survey, introduction

  2. Plant Transcription Factor

    Yoko Mizuta, Katsutoshi Tsuda, Yamaguchi Nobutoshi( Role: Contributor ,  Three-dimensional multiphoton imaging of transcription factor by ClearSee.)

    Springer protocols  2018.7  ( ISBN:9781493986569

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    Responsible for pages:257-268   Language:English Book type:Scholarly book

  3. THE SECRET LIFE OF PLANTS

    Daisuke Kurihara and Yoko Mizuta( Role: Joint author)

    National geographic  2017.4 

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    Language:English Book type:Image

    Other Link: https://www.amazon.co.jp/National-Geographic-US-May-2017/dp/B072L2RDWM

  4. 植物の中まで丸見え

    栗原 大輔、水多 陽子( Role: Joint author)

    ナショナルジオグラフィック 日本版  2016.6 

  5. 植物の透明化

    東山 哲也、栗原 大輔、水多 陽子( Role: Joint author)

    太田出版  2016.4  ( ISBN:9784778315160

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    Language:Japanese Book type:Image

MISC 2

  1. 特集「異分野融合が推進するイメージング研究」

    水多陽子、多喜正泰

    Plant Morphology   Vol. 33   page: 1 - 2   2021.5

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    Authorship:Lead author, Corresponding author   Language:Japanese  

  2. 卓上型走査型電子顕微鏡によるシロイヌナズナめしべの観察像

    水多 陽子, 栗原 大輔

    PLANT MORPHOLOGY   Vol. 29 ( 1 ) page: 0-0   2017

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    Authorship:Lead author   Language:Japanese  

    <p>卓上型走査型電子顕微鏡によるシロイヌナズナめしべの観察像 めしべの片側の子房壁を取り除いて,キーエンスVHX-2000 およびVHX-D510 により撮影.</p>

    DOI: 10.5685/plmorphol.29.0

    CiNii Article

Presentations 64

  1. イネにおける成長相転換とジベレリン生合成の時空間的解析

    (12) 新美陽子, 永井啓祐, 保浦徳昇, 水多陽子, 竹林 裕美子, 小嶋美紀子, 榊原均, 島谷善平, 寺田理枝, 辻寛之, 芦苅基行

    日本育種学会第140回講演会 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:弘前大学  

  2. 花粉管を用いた生殖細胞のゲノム編集と導入細胞の可視化

    永原史織, 東山哲也, 水多陽子

    日本植物学会第85回大会  2021.9.9 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京都立大学  

  3. 花粉管を用いたゲノム編集酵素のデリバリーと生殖細胞の遺伝子改変 Invited

    水多陽子

    第38回日本植物バイオテクノロジー学会 シンポジウム「Made in Japan:世界を駆ける日本発のゲノム編集技術開発の最前線」  2021.9.11 

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    Event date: 2021.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:筑波大学  

  4. 花粉管発生におけるライブイメージングと生殖細胞の改変

    水多陽子

    第62回日本植物生理学会年会・関連集会「植物生殖改変ワークショップ」  2021.3.14 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  5. イネ花粉成熟期における糖化機構の解明

    (9) 葉山旺,河合恭甫,杉原諒彦,森井南美,竹原清日,水多陽子,上口美弥子

    第62回日本植物生理学会年会  2021.3.14 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:島根大学  

  6. 観る、知る、利用する。植物の花に秘められたいのちのしくみ Invited

    水多陽子

    第7回名古屋大学の卓越・先端・次世代 研究シンポジウム「学問の意義と貢献:研究のインパクトと社会との対話」  2021.1.22 

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    Event date: 2021.1

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  7. 極彩色で観る 植物がいのちをつなぐしくみ Invited

    水多陽子

    サイエンスカフェ(KMI x ITbM Mix Cafe)集まれ!話そう!科学のワクワク!  2020.9.30 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  8. 花粉管誘引において同種認証機構を担う雌しべ組織の検討

    長江拓也, 武内秀憲, 水多陽子, 須崎大地, 東山哲也

    日本植物学会第84回大会  2020.9.21 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

  9. Ca2+に着目した花粉管の破裂制御における種認証機構の解析

    五郎丸輝明, 長江拓也, 水多陽子, 東山哲也

    日本植物学会第84回大会  2020.9.21 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

  10. 花粉不発芽を引き起こすDPL遺伝子の機能解析

    水多陽子, 栗原大輔

    日本植物学会第84回大会  2020.9.19  日本植物学会

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  11. コロナ禍における植物研究者の変容と進化 Invited

    水多陽子

    第1回 名古屋大学高等研究院ウェビナー(高等研究院×未来社会創造機構) 「未来を見据えるコロナ禍の研究者たち」  2020.6.2 

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    Event date: 2020.6

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  12. 花粉管をベクターとしたゲノム編集酵素のデリバリー Invited

    水多陽子

    プロジェクト横断型公開シンポジウム「植物のゲノム編集基盤技術開発の現状と展望」 

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    Event date: 2020.2

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  13. 「花のなかを観る」植物のタネができるしくみ Invited

    水多陽子

    次世代研究者シンポジウム2019  2019.10.31 

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    Event date: 2019.10

    Language:English   Presentation type:Oral presentation (general)  

  14. 花粉管破裂制御におけるCa2+動態と種認証機構の関係

    五郎丸輝明,東山哲也,水多陽子,長江拓也

    日本植物学会第83回大会 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  15. 深部イメージングで探る植物生殖の謎

    水多陽子, 栗原大輔, 東山 哲也

    日本植物学会第83回大会  

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    Event date: 2019.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  16. 二光子イメージングによる一対一受精機構の解明

    水多 陽子, 栗原 大輔, 東山 哲也

    日本植物形態学会第31回大会 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  17. 花粉管をベクターとした生殖細胞の遺伝子改変 Invited

    水多陽子、永原史織、東山哲也

    日本育種学会第136回講演会 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  18. 花粉への一過的遺伝子導入系による花粉管および精細胞の動態解析

    永原史織, 栗原大輔, 東山哲也, 水多陽子

    第60回日本植物生理学会年会  2019.3.14 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  19. 花粉管をベクターとした生殖細胞の遺伝子改変と植物生殖機構の解明

    水多陽子, 永原史織, 栗原大輔, 東山哲也

    第60回日本植物生理学会年会 シンポジウム  2019.3.13 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  20. 二光子励起顕微鏡で解く植物生殖の謎

    水多陽子

    第7回 植物イメージングの会  2019.2.22 

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    Event date: 2019.2

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  21. 花粉のゲノム編集

    水多陽子, 永原史織, 東山哲也

    日本植物学会第82回大会  2018.9.16 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  22. 植物透明化試薬ClearSeeの改良

    栗原大輔, 水多陽子, 永原史織, 東山哲也

    日本植物学会第82回大会  2018.9.15 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  23. 花粉のゲノム編集法

    水多陽子, 永原史織, 東山哲也

    日本植物形態学会第30回大会  2018.9.13 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  24. さらなる植物透明化に向けて

    栗原大輔, 水多陽子, 永原史織, 東山哲也

    日本植物形態学会第30回大会  2018.9.13 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  25. Two-photon imaging reveals spatio-temporal regulation on the one-to-one pollen tube guidance International conference

    Yoko Mizuta, Daisuke Kurihara, Shiori Nagahara, Tetsuya Higashiyama

    The 25th International Congress on Sexual Plant Reproduction (ICSPR2018)  2018.6.12 

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    Event date: 2018.6

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  26. パーティクルボンバードメント法による植物生殖細胞へのCRISPR/Cas9の導入

    永原 史織, 栗原 大輔, 東山 哲也, 水多 陽子

    2017年度生命科学系学会合同年次大会(ConBio2017)  2017.12.9 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  27. 生体深部イメージングで解く花粉管ガイダンスの謎 Invited

    水多陽子

    2017年度生命科学系学会合同年次大会(ConBio2017)  2017.12.9 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  28. より深く、詳しく、美しく― 生きた植物の内部を観る Invited

    水多陽子

    第2回フロンティアバイオイメージング研究会  2017.11.7 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  29. 3Dプリンタを用いた植物組織の新しい3D画像データ提示手法の開発

    小笠原希実, 水多陽子, 東山哲也

    第58回 日本植物生理学会年会  2017.3.17 

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  30. 植物深部に侵入した微生物を光学顕微鏡で観察する

    栗原大輔, 大津美奈, 水多陽子, 東山哲也

    日本植物学会 第80回大会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  31. ClearSee で観る植物生殖のメカニズム

    水多陽子, 栗原大輔, 東山哲也

    日本植物学会 第80回大会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  32. Live imaging of membrane traffic in Arabidopsis pollen tubes

    Live imaging of membrane traffic in Arabidopsis pollen tubes 

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    Event date: 2016.6

    Presentation type:Poster presentation  

    Country:Japan  

  33. 花粉管と胚珠の相互作用に関する シミュレーション解析 International conference

    鈴木孝征、水多陽子、東山哲也

    第 57 回 日本植物生理学会年会 

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    Event date: 2016.3

    Presentation type:Oral presentation (general)  

    Country:Japan  

  34. 深部観察で Whole plant imaging を目指す

    栗原大輔、水多陽子、東山哲也

    日本植物学会第79回大会 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  35. 一本の花粉管が一つの胚珠にたどりつくには? 〜深部イメージングで探る花粉管ガイダンスの謎〜 Invited

    水多陽子、栗原大輔、東山哲也

    日本植物学会第79回大会 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  36. Two-photon imaging of pollen tube guidance in the Arabidopsis pistil

    Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama

    NIG Biological Symposium 

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    Event date: 2015.2

    Language:English  

    Country:Japan  

  37. 2光子顕微鏡を用いた花粉管ガイダンスのin vivoライブイメージング

    水多陽子、栗原大輔、東山哲也

    日本植物学会第78回大会 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  38. 生体深部イメージングによる花粉管ガイダンスの「形」と「通り道」の解析

    水多陽子、栗原大輔、東山哲也

    日本植物形態学会第26回大会 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  39. Deep imaging of pollen tube guidance by two-photon microscopy International conference

    Yoko Mizuta

    Deep imaging of pollen tube guidance by two-photon microscopy 

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    Event date: 2014.7

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  40. Two-photon imaging of pollen tube growth and guidance in the pistil International conference

    Y Mizuta, D Kurihara, T Higashiyama

    23rd ICSPR: International Conference on Sexual Plant Reproduction 

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    Event date: 2014.7

    Language:English   Presentation type:Poster presentation  

    Country:Portugal  

  41. 2光子顕微鏡を用いた花粉管の受精競争と多精拒否の in vivo ライブイメージング

    水多陽子、栗原大輔、東山哲也

    動植物に共通する アロ認証機構の解明 第8回領域会議 

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    Event date: 2014.1

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  42. 2 光子顕微鏡による深部イメージングで観えてきた植物生殖の実態 Invited

    水多陽子、栗原大輔、東山哲也

    日本植物学会第77回大会 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  43. 花粉管のin vivoイメージングで観えてきた植物生殖の実態 -2光子顕微鏡を用いたアプローチ-

    水多陽子、栗原大輔、東山哲也

    日本植物形態学会第 25 回大会 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  44. 植物生殖プロセスにおける花粉管ガイダンスのオンチップ定量解析

    佐藤良勝, 洞出光洋, 水多陽子, 加地範匡, 東山哲也, 新田英之

    日本分析化学会年会講演要旨集  2013.8.27 

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    Event date: 2013.8

    Language:Japanese   Presentation type:Oral presentation (general)  

  45. Phosphorothioate antisense oligodeoxynucleotides to transiently supress gene expression in living pollen tubes International conference

    Y Mizuta, T Higashiyama

    24th International Conference on Arabidopsis Research (ICAR 2013) 

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    Event date: 2013.6

    Language:English   Presentation type:Poster presentation  

    Country:Australia  

  46. マイクロ流路を用いた花粉管細胞配列アッセイ

    洞出光洋, 水多陽子, 東山哲也, 新田英之

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2013.5.23 

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    Event date: 2013.5

    Language:Japanese   Presentation type:Oral presentation (general)  

  47. マイクロ流路デバイスを用いた花粉管誘引物質の1分子ライブイメージング

    水多陽子, 洞出光洋, 後藤宏旭, 加地範匡, 新田英之, 東山哲也

    日本植物学会第76回大会 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  48. 花粉管ガイダンスの分子実体解明に向けてーマイクロ流体デバイスを用いた試み

    水多陽子, 洞出光洋, 後藤宏旭, 加地範匡, 新田英之, 東山哲也

    日本植物形態学会第24回大会 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  49. PDMSマイクロチャネルを用いた花粉管細胞伸長の計測と制御

    洞出光洋, 水多陽子, 加地範匡, 新田英之, 東山哲也

    化学とマイクロ・ナノシステム研究会講演要旨集  2012.5.17 

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    Event date: 2012.5

    Language:Japanese   Presentation type:Oral presentation (general)  

  50. 花粉管内標的遺伝子の機能阻害と花粉管誘引アッセイに対する新アプローチ

    水多陽子, 洞出光洋, 加地範匡, 後藤宏旭, 新田英之, 東山哲也

    第53回日本植物生理学会年会 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  51. イネの生殖的隔離およびシロイヌナズナの生殖に関わる花粉側因子の同定

    水多陽子, 東山哲也, 春島嘉章, 倉田のり

    日本植物学会第75回大会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  52. S化オリゴを用いた花粉内標的遺伝子の機能阻害と受精に関わる花粉側因子の探索

    水多陽子, 東山哲也

    日本植物形態学会第23回大会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  53. イネ亜種間交雑で生殖的隔離を引き起こす重複遺伝子DPL1, 2の解析 Invited

    水多 陽子

    日本遺伝学会第82回大会 

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    Event date: 2010.9

    Language:Japanese  

    Country:Japan  

  54. イネ亜種間交雑で生殖的隔離を引き起こすDOPPELGANGER(DPL)1とDPL2の解析

    水多陽子, 春島嘉章, 倉田のり

    日本育種学会第117回講演会 

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    Event date: 2010.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  55. イネ亜種間交雑で生殖的隔離を引き起こすDOPPELGANGER(DPL)1およびDPL2遺伝子の解析

    水多陽子, 春島嘉章, 倉田のり

    第51回日本植物生理学会年会 

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    Event date: 2010.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  56. Analysis of a pair of genes, DOPPELGANGER(DPL)1 and DPL2 responsible for reproductive isolation between two rice subspecies International conference

    Yoko Mizuta, Yoshiaki Harushima, Nori Kurata

    International Symposium of Cell-Cell Communication in Plant Reproduction : from pollination to fertilization 

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    Event date: 2010.3

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  57. イネ亜種間交雑で生殖的隔離を引き起こすDOPPELGANGER1(DPL1)とDPL2の単離・解析

    水多陽子, 春島嘉章, 倉田のり

    第32回日本分子生物学会年会 

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    Event date: 2009.12

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  58. イネ亜種間交雑において生殖的隔離を引き起こす相互作用遺伝子座DOPPELGANGER1と2の単離・解析

    水多陽子, 春島嘉章, 倉田のり

    日本遺伝学会第81回大会 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  59. イネ亜種間交雑で生殖的隔離障壁となる重複遺伝子の解析

    水多陽子, 春島嘉章, 倉田のり

    日本育種学会114回講演会 

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    Event date: 2008.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  60. Positional Cloning of a Pair of Interactive Genes Causing Reproductive Barrier in the Hybrid Pollen of Rice International conference

    Yoko Mizuta, Yoshiaki Harushima, Nori Kurata

    XX International congress of Genetics 

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    Event date: 2008.7

    Language:English   Presentation type:Poster presentation  

    Country:Germany  

  61. イネ雑種花粉で作用する生殖的隔離障壁遺伝子のポジショナルクローニング

    水多陽子, 春島嘉章, 倉田のり

    第112回育種学会 

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    Event date: 2007.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  62. イネ雑種花粉で相互作用する2遺伝子座に起因する生殖的隔離

    水多陽子, 春島嘉章, 倉田のり

    日本遺伝学会第79回大会 

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    Event date: 2007.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  63. Production of interspecific and intergeneric hybrids among Colchicaceous ornamentals via ovule culture : 6. Characterization of intergeneric hybrid plants of Sandersonia aurantiaca × Gloriosa superba 'Lutea'

    AMANO J, KUWAYAMA S, SUGAWARA S, MIZUTA Y, GODO T, NAKANO M

    園芸学研究. 別冊, 園芸学会大会研究発表要旨  2007.3.24 

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    Event date: 2007.3

    Language:Japanese   Presentation type:Oral presentation (general)  

  64. Production of interspecific and intergeneric hybrids among Colchicaceous ornamentals via ovule culture : 4. Characterization of plants derived from Littonia modesta × Sandersonia aurantiaca

    KUWAYAMA S, AMANO J, SUGAWARA S, NAKAMURA T, MIZUTA Y, OOMIYA T, NAKANO M

    園芸学会雑誌. 別冊, 園芸学会大会研究発表  2005.10.1 

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    Event date: 2005.10

    Language:Japanese   Presentation type:Oral presentation (general)  

▼display all

Works 4

  1. めしべを丸ごと透明化(科学技術の美パネル展)優秀賞

    水多 陽子

    2016

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    Work type:Artistic work   Location:科学技術の美パネル展  

  2. Where the Magic Happens (Cell Picture Show)

    Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama

    2015

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    Work type:Artistic work   Location:Cell Picture Show: The Biology of Sex  

  3. ロゴマーク ユキツバキ(日本植物学会第79回大会)

    水多 陽子

    2015

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    Work type:Artistic work   Location:日本植物学会第79回大会  

  4. 生きている花粉管の花火(科学技術の美パネル展)

    水多 陽子

    2014

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    Work type:Artistic work   Location:科学技術の美パネル展  

Research Project for Joint Research, Competitive Funding, etc. 6

  1. イメージングと遺伝子発現を基盤とした植物の受精に必須な遺伝子の解析

    2020.12 - 2022.3

    公益財団法人 木下記念事業団 学術研究活動助成事業 

    水多陽子

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    Grant type:Competitive

  2. 花粉による新植物育種技術の開発

    2019.10

    研究成果展開事業 A-STEP 産学共同フェーズ(シーズ育成タイプ) 

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    Grant type:Competitive

  3. RNPを導入した花粉による新育種技術の開発

    2018.10 - 2019.9

    研究成果展開事業 A-STEP 産学共同フェーズ(シーズ育成タイプFS) 

    水多陽子、他1名

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    Grant type:Competitive

  4. 花粉管をベクターとした遺伝子改変技術の開発

    2016.4 - 2019.3

    JST さきがけ 

    水多陽子

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    Grant type:Competitive

  5. 一分子ライブイメージングによる花粉管誘引ペプチドと花粉管の相互作用の解析

    2015.4 - 2016.3

    公益財団法人 旭硝子財団 研究助成 第1分野 (化学・生命科学系) 奨励研究 

    水多陽子

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    Grant type:Competitive

  6. In vivoカルシウムイメージングによる雌雄細胞間の情報伝達機構の解析

    2015.4 - 2016.3

    光科学技術研究振興財団 研究助成(第二課題) 

    水多陽子

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    Grant type:Competitive

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KAKENHI (Grants-in-Aid for Scientific Research) 5

  1. Remodeling plant reproduction system by cell fate manipulations.

    Grant number:20H05778  2020.10 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (B)

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    Authorship:Coinvestigator(s) 

  2. Molecular mechanism of cell fate and fertility determination of pollen sperm cells by single-cell tracking

    Grant number:20H05779  2020.10 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (B)

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    Authorship:Principal investigator 

    Grant amount:\47710000 ( Direct Cost: \36700000 、 Indirect Cost:\11010000 )

  3. 雌雄のクロストークによる花粉管誘引のON/OFFスイッチの解明

    Grant number:18K14741  2018.4 - 2020.3

    日本学術振興会  科学研究費助成事業 若手研究 (B)  若手研究(B)

    水多 陽子

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    Authorship:Principal investigator  Grant type:Competitive

  4. In vivoライブイメージングと遺伝学の融合による植物受精機構の動的理解

    Grant number:26840104  2014.4 - 2016.3

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

  5. イネ亜種間交雑における生殖的隔離障壁因子の単離及び機能解析

    Grant number:18075009  2007.4 - 2010.3

    科学研究費補助金 

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    Authorship:Principal investigator 

Industrial property rights 2

  1. 花粉への物質導入方法

    栗原(水多)陽子, 皆川吉, 永原史織

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    Date applied:2019.4

    Announcement no:2020-171262  Date announced:2020.10

    Country of applicant:Domestic   Country of acquisition:Domestic

  2. 植物ゲノム編集方法

    栗原(水多)陽子, 永原史織

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    Applicant:国立大学法人名古屋大学

    Application no:特願2018-79316  Date applied:2018.4

    Announcement no:2019-180373  Date announced:2019.10

    Patent/Registration no:特許第6614622号  Date registered:2019.11  Date issued:2019.12

    Country of applicant:Domestic  

 

Teaching Experience (On-campus) 1

  1. Ph.D. Professional, YM course ヤングメンター制度「花粉の顕微鏡による観察と遺伝子発現」

    2020

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    生物の生殖過程では、雄と雌の細胞のダイナミックな動態が見られる。本コースでは、そのうち植物の花粉と呼ばれる雄の生殖細胞に焦点を当てる。様々な植物の花粉を観察し、一過的な遺伝子発現を行うことで、種の多様性と植物の生殖について理解を深める。こうした解析を通じて、細胞培養や顕微鏡観察の技術についても学ぶ。

 

Social Contribution 2

  1. 「光らせよう!見てみよう!蛍光たんぱく質で科学者体験」

    Role(s):Lecturer

    サカエ大学Common-S.(運営:松坂屋名古屋店)  松坂屋小学校 第13回キッズサイエンス  名古屋大学 東山キャンパス NIC館  2021.11

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    Audience: Infants, Schoolchildren

    Type:Seminar, workshop

  2. 花の神秘 隠されたいのちのしくみ

    Role(s):Appearance, Lecturer

    サカエ大学 Common-S.(運営:松坂屋名古屋店)、名古屋大学 学術研究・産学官連携推進本部  名大カフェ "Science, and Me "  2021.4

Media Coverage 6

  1. めしべの色彩 花火のよう Newspaper, magazine

    読売新聞社  読売新聞  夕刊4版  2021.2

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    Author:Other 

  2. THE SECRET LIFE OF PLANTS Newspaper, magazine

    National Geographic Society  National Geographic Magazine  page 26  2017.4

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    Author:Other 

  3. 植物の透明化 Newspaper, magazine

    太田出版  ケトル Vol.30  135ページ  2016.4

  4. 内部構造がまる見え! 植物を透明にする新技術を開発 Newspaper, magazine

    誠文堂新光社  子供の科学  4ページ  2015.12

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    Author:Other 

  5. Where the Magic Happens Internet

    Cell Press  Cell Picture Show, The Biology of Sex  page 10  2015

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    Author:Other 

  6. 組み換え技術使わず 名大 花粉管の遺伝子を制御 Newspaper, magazine

    科学新聞社  科学新聞  4面  2014.3

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