Updated on 2024/09/24

写真a

 
MIZUTA Yoko
 
Organization
Institute of Transformative Bio-Molecules Assistant Professor
Title
Assistant Professor
External link

Degree 1

  1. 博士（理学） （ 2010.3   総合研究大学院大学 ） 

To the head of Degree.▲

Research Interests 10

  1. 植物生殖

  2. イメージング

  3. 花粉管

  4. ゲノム編集

  5. 二光子励起顕微鏡

  6. 生殖的隔離

  7. 植物分子生物学

  8. 花粉

  9. Development

  10. 非対称分裂

To the head of Research Interests.▲

Research Areas 4

  1. Life Science / Morphology and anatomical structure

  2. Life Science / Plant molecular biology and physiology

  3. Environmental Science/Agriculture Science / Science in plant genetics and breeding

  4. Life Science / Cell biology

To the head of Research Areas.▲

Current Research Project and SDGs 3

  1. イメージングを基盤とした植物の生殖機構の解明

  2. 花粉による新植物育種技術の開発

  3. 花粉の発生と受精能を決定する分子メカニズムの解明

To the head of Current Research Project and SDGs.▲

Research History 7

  1. Nagoya University   ITbM   Assistant Professor

    2024.4

  2. Nagoya University   Institute of Transformative Bio-Molecules   Jr. PI

    2022.9

  3. Nagoya University

    2019.4

    　 More details

    Country：Japan

  4. JST   ITbM, Nagoya University   JST PRESTO

    2016.4 - 2019.3

    　 More details

    Country：Japan

  5. 名古屋大学大学院   理学研究科　ERATO東山ライブホロニクスプロジェクト   研究員

    2011.4 - 2016.3

    　 More details

    Country：Japan

  6. 情報・システム研究機構　国立遺伝学研究所   植物遺伝研究室   特任研究員

    2010.4 - 2011.3

    　 More details

    Country：Japan

  7. 日本学術振興会特別研究員DC1

    2007.4 - 2010.3

    　 More details

    Country：Japan

▼display all

To the head of Research History.▲

Education 2

  1. The Graduate University for Advanced Studies

    2005.4 - 2010.3

    　 More details

    Country： Japan

  2. Niigata University   Faculty of Agriculture

    2001.4 - 2005.3

    　 More details

    Country： Japan

To the head of Education.▲

Professional Memberships 3

  1. THE JAPANESE SOCIETY OF PLANT MORPHOLOGY

  2. THE BOTANICAL SOCIETY OF JAPAN

  3. THE JAPANESE SOCIETY OF PLANT PHYSIOLOGISTS

To the head of Professional Memberships.▲

Committee Memberships 4

  1. The Japanese Society of Plant Physiologists   Plant Science New Technology Working Group  

    2023.4   

    　 More details

    Committee type：Academic society

  2.   The 84th Annual Meeting of the Botanical Society of Japan  

    2020.9   

    　 More details

    Committee type：Academic society

  3. Frontiers in Genome editing   Review Editor, Editorial Board of Genome Editing in Plants  

    2020.7   

    　 More details

    Committee type：Academic society

  4. 文部科学省 科学技術・学術政策研究所 科学技術予測センター（NISTEP）   専門調査員  

    2020.4 - 2021.3   

To the head of Committee Memberships.▲

Awards 11

  1. Hirase Award

    2024.9   The Japanese Society of Plant Morphology   Deep imaging reveals dynamics and signaling in one-to-one pollen tube guidance

    Yoko Mizuta, Daigo Sakakibara, Shiori Nagahara, Ikuma Kaneshiro, Takuya T Nagae, Daisuke Kurihara, Tetsuya Higashiyama

    　More details

    Award type：Award from Japanese society, conference, symposium, etc. 

  2. Best presentation

    2021.9   The 140th Meeting of the Japanese Society of Plant Breeding   Spatiotemporal analysis of growth phase transition and gibberellin biosynthesis in rice

    　More details

    Award type：Award from Japanese society, conference, symposium, etc. 

  3. NIKON JOICO AWARD 2019 優秀賞

    2020.1   株式会社ニコンインステック   花のなかの秘密

    水多陽子

    　More details

    Country：Japan

  4. Best presentation

    2018.12   The 4th Annual Meeting of the Agricultural Nakate symposisum   Pollen tube mediated plant modification

    Yoko Mizuta

    　More details

    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

  5. Second prize

    2017.4   The 11th Panel Exhibition of Beauty of Science and Technology   Deep imaging of Arabidopsis thaliana pollinated pistil using plant transparency reagent

    Yoko Mizuta

    　More details

    Country：Japan

  6. 平瀬賞

    2016.9   日本植物形態学会   ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging. Development 142: 4168-4179

    D. Kurihara, Y. Mizuta, Y. Sato and T. Higashiyama.

    　More details

    Award type：Honored in official journal of a scientific society, scientific journal  Country：Japan

    http://square.umin.ac.jp/pl-morph/pdf/170810_Hirase_papers.pdf

  7. Cell Picture Show (顕微鏡写真コンテスト、ZEISS社 2016年 国際カレンダー採用）

    2016   Cell Press and Zeiss   Where the Magic Happens

    Yoko Mizuta, Daisuke Kurihara Tetsuya Higashiyama

    　More details

    Country：Germany

    https://www.cell.com/pictureshow/biology-of-sex

  8. 日本植物学会第79回大会 ロゴマーク採用

    2014.11   日本植物学会第79回大会   ユキツバキ

    水多陽子

    　More details

    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

    http://bsj.or.jp/jpn/members/information/622.php

  9. 最優秀ポスター賞

    2014.1   新学術領域研究　動植物に共通するアロ認証機構の解明　第8回領域会議   2光子顕微鏡を用いた花粉管の受精競争と多精拒否のin vivoライブイメージング

    水多陽子

    　More details

    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

  10. 優秀発表賞

    2010.3   日本育種学会第117回講演会   イネ亜種間交雑で生殖的隔離を引き起こすDOPPELGANGER(DPL)1とDPL2の解析

    水多 陽子、春島 嘉章、倉田 のり

    　More details

    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

    https://www.nacos.com/jsb/07/07prize_2010.html

  11. Best Paper賞

    2009.9   日本遺伝学会第81回大会   イネ亜種間交雑において生殖的隔離を引き起こす相互作用遺伝子座DOPPELGANGER1と2の単離・解析

    水多陽子、春島嘉章、倉田のり

    　More details

    Award type：Award from Japanese society, conference, symposium, etc.  Country：Japan

▼display all

To the head of Awards.▲
 

Papers 35

  1. Deep imaging reveals dynamics and signaling in one-to-one pollen tube guidance Reviewed

    Yoko Mizuta, Daigo Sakakibara, Shiori Nagahara, Ikuma Kaneshiro, Takuya T Nagae, Daisuke Kurihara, Tetsuya Higashiyama

    EMBO Reports   Vol. 25 ( 6 ) page： 2529 - 2549   2024.6

    　More details

    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    In the pistil of flowering plants, each ovule usually associates with a single pollen tube for fertilization. This one-to-one pollen tube guidance, which contributes to polyspermy blocking and efficient seed production, is largely different from animal chemotaxis of many sperms to one egg. However, the functional mechanisms underlying the directional cues and polytubey blocks in the depths of the pistil remain unknown. Here, we develop a two-photon live imaging method to directly observe pollen tube guidance in the pistil of Arabidopsis thaliana, clarifying signaling and cellular behaviors in the one-to-one guidance. Ovules are suggested to emit multiple signals for pollen tubes, including an integument-dependent directional signal that reaches the inner surface of the septum and adhesion signals for emerged pollen tubes on the septum. Not only FERONIA in the septum but ovular gametophytic FERONIA and LORELEI, as well as FERONIA- and LORELEI-independent repulsion signal, are involved in polytubey blocks on the ovular funiculus. However, these funicular blocks are not strictly maintained in the first 45 min, explaining previous reports of polyspermy in flowering plants.

    DOI： 10.1038/s44319-024-00151-4

    Web of Science

    Scopus

    PubMed

    Other Link： https://www.nagoya-u.ac.jp/researchinfo/result/2024/05/post-665.html

  2. Blue Light Irradiation Induces Pollen Tube Rupture in Various Flowering Plants Reviewed

    Naoya Sugi, Daichi Susaki, Yoko Mizuta, Tetsu Kinoshita, Daisuke Maruyama

    Plant And Cell Physiology   Vol. 65 ( 5 ) page： 704 - 707   2024.5

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    DOI： 10.1093/pcp/pcae018

    Web of Science

    Scopus

    PubMed

  3. Blue light irradiation induces pollen tube rupture in various flowering plants

    Naoya Sugi, Daichi Susaki, Yoko Mizuta, Tetsu Kinoshita, Daisuke Maruyama

    bioRxiv     2023.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1101/2023.12.06.570347

  4. Deep imaging revealed dynamics and signaling in one-to-one pollen tube guidance

    Yoko Mizuta, Daigo Sakakibara, Shiori Nagahara, Ikuma Kaneshiro, Takuya T. Nagae, Daisuke Kurihara, Tetsuya Higashiyama

    bioRxiv     2023.4

    　More details

    Authorship：Lead author,　Corresponding author   Publisher：Cold Spring Harbor Laboratory  

    ABSTRACT

    In angiosperms, pollen tube guidance allows sperm cell delivery to the female gametes within the ovule, which are deeply embedded in a flower. However, when an ovary includes multiple pollen tubes and ovules, it is unclear how each ovule is fertilized one-to-one by a pollen tube. Here, our two-photon imaging revealed the pollen tube dynamics in living ovaries. The number of pollen tubes and ovule maturity affected the target selection among multiple ovules. On the inner surface of the septum epidermis within the transmitting tract, pollen tube behavior and emergence were regulated by the ovular sporophytic signals. In funicular guidance, the second pollen tube was strictly repelled by the FERONIA and LORELEI-dependent gametophytic signal, especially more than 45 minutes after the first pollen tube had passed. Such highly spatiotemporal regulation mechanisms in the one-to-one pollen tube guidance may allow angiosperms to produce more offspring in nature.

    DOI： 10.1101/2023.04.19.537439

  5. Biolistic-delivery-based pollen visualization and genome editing Reviewed

    Yoko Mizuta

    BSJ-Review   Vol. 14 ( A ) page： 21 - 29   2023.3

    　More details

    Authorship：Lead author,　Last author,　Corresponding author   Language：Japanese   Publisher：THE BOTANICAL SOCIETY OF JAPAN  

    DOI： 10.24480/bsj-review.14a4.00239

    CiNii Research

  6. 植物細胞の分化運命の制御と可塑性 Reviewed

    丸山 大輔, 水多 陽子, 山岡 尚平

    植物科学の最前線（BSJ-Review）   Vol. 14 ( A ) page： 1 - 2   2023

    　More details

    Language：Japanese   Publisher：公益社団法人 日本植物学会  

    DOI： 10.24480/bsj-review.14a1.00236

    CiNii Research

  7. Target pollen isolation using automated infrared laser-mediated cell disruption Reviewed

    Ikuma Kaneshiro, Masako Igarashi, Tetsuya Higashiyama, Yoko Mizuta

    Quantitative Plant Biology   Vol. 3   page： e30   2022.12

    　More details

    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Cambridge University Press (CUP)  

    Abstract

    Single-cell analysis is important to understand how individual cells work and respond at the cell population level. Experimental single-cell isolation techniques, including dilution, fluorescence-activated cell sorting, microfluidics, and micromanipulation, have been developed in recent decades. However, such applications typically require large cell populations and skilled professionals. Additionally, these methods are unsuitable for sequential analysis before and after cell isolation. In this study, we propose a method for target cell isolation using automated infrared laser-mediated disruption of pollen grains in pollen populations. Germination of the target pollen was observed at the same location as that before laser irradiation, and germinated pollen grains were enriched in the cell population. Pollination of laser-irradiated bulk pollen populations also showed that the target pollen preferentially germinated on the stigma. This method is expected to facilitate physiological analyses of target cells at the single-cell level and effectively produce seeds derived from target pollen.

    DOI： 10.1017/qpb.2022.24

    Web of Science

    Scopus

    PubMed

    Other Link： https://www.microscope.healthcare.nikon.com/ja_JP/resources/application-notes/automatic-isolation-of-pollen-using-nis-elements-general-analysis-ga-and-jobs-imaging-workflow-tools

  8. Deep fluorescence observation in rice shoots via clearing technology Reviewed International journal

    Yoko Niimi, Keisuke Nagai, Motoyuki Ashikari, Yoko Mizuta

    Journal of Visualized Experiments   Vol. 2022 ( 184 )   2022.6

    　More details

    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    The recently developed clearing technology that eliminates refractive index mismatches and diminishes auto-fluorescent material has made it possible to observe plant tissues in three dimensions (3D) while preserving their internal structures. In rice (Oryza sativa L.), a monocot model plant and a globally important crop, clearing technology has been reported in organs that are relatively easy to observe, such as the roots and leaves. Applications of clearing technology in shoot apical meristem (SAM) and stems have also been reported, but only to a limited degree because of the poor penetration of the clearing solution (CS) in these tissues. The limited efficiency of the clearing solutions in these tissues has been attributed to auto-fluorescence, thickening, and hardening of the tissues in the stem as the vascular bundles and epidermis develop and layering of the SAM with water-repellent leaves. The present protocol reports the optimization of a clearing approach for continuous and 3D observation of gene expression from the SAM/young panicle to the base of the shoots during development. Fixed tissue samples expressing a fluorescent protein reporter were trimmed into sections using a vibrating micro-slicer. When an appropriate thickness was achieved, the CS was applied. By specifically targeting the central tissue, the penetration rate and uniformity of the CS increased, and the time required to make the tissue transparent decreased. Additionally, clearing of the trimmed sections enabled the observation of the internal structure of the whole shoot from a macro perspective. This method has potential applications in deep imaging of tissues of other plant species that are difficult to clear.

    DOI： 10.3791/64116

    Web of Science

    Scopus

    PubMed

  9. Optical Clearing of Plant Tissues for Fluorescence Imaging Reviewed

    Daisuke Kurihara, Yoko Mizuta, Shiori Nagahara, Yoshikatsu Sato, Tetsuya Higashiyama

    Journal of Visualized Experiments   Vol. 2021 ( 179 )   2022.1

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：MyJove Corporation  

    DOI： 10.3791/63428

    Web of Science

    Scopus

    PubMed

  10. Detection of a biolistic delivery of fluorescent markers and CRISPR/Cas9 to the pollen tube Reviewed

    Shiori Nagahara, Tetsuya Higashiyama, Yoko Mizuta

    Plant Reproduction   Vol. 34 ( 3 ) page： 191 - 205   2021.9

    　More details

    Authorship：Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

    Abstract

    Key message

    Biolistic delivery into pollen. Abstract

    In recent years, genome editing techniques, such as the CRISPR/Cas9 system, have been highlighted as a new approach to plant breeding. Agrobacterium-mediated transformation has been widely utilized to generate transgenic plants by introducing plasmid DNA containing CRISPR/Cas9 into plant cells. However, this method has general limitations, such as the limited host range of Agrobacterium and difficulties in tissue culture, including callus induction and regeneration. To avoid these issues, we developed a method to genetically modify germ cells without the need for Agrobacterium-mediated transfection and tissue culture using tobacco as a model. In this study, plasmid DNA containing sequences of Cas9, guide RNA, and fluorescent reporter was introduced into pollen using a biolistic delivery system. Based on the transient expression of fluorescent reporters, the Arabidopsis UBQ10 promoter was found to be the most suitable promoter for driving the expression of the delivered gene in pollen tubes. We also evaluated the delivery efficiency in male germ cells in the pollen by expression of the introduced fluorescent marker. Mutations were detected in the target gene in the genomic DNA extracted from CRISPR/Cas9-introduced pollen tubes, but were not detected in the negative control. Bombarded pollen germinated pollen tubes and delivered their contents into the ovules in vivo. Although it is necessary to improve biolistic delivery efficiency and establish a method for the screening of genome-modified seeds, our findings provide important insights for the detection and production of genome-modified seeds by pollen biolistic delivery.

    DOI： 10.1007/s00497-021-00418-z

    Web of Science

    Scopus

    PubMed

    Other Link： https://link.springer.com/article/10.1007/s00497-021-00418-z/fulltext.html

  11. ClearSeeAlpha: Advanced Optical Clearing for Whole-Plant Imaging Reviewed

    Daisuke Kurihara, Yoko Mizuta, Shiori Nagahara, Tetsuya Higashiyama

    Plant and Cell Physiology   Vol. 62 ( 8 ) page： 1302 - 1310   2021.8

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    To understand how the body of plants is made, it is essential to observe the morphology, structure and arrangement of constituent cells. However, the opaque nature of the plant body makes it difficult to observe the internal structures directly under a microscope. To overcome this problem, we developed a reagent, ClearSee, that makes plants transparent, allowing direct observation of the inside of a plant body without inflicting damage on it, e.g. through physical cutting. However, because ClearSee is not effective in making some plant species and tissues transparent, in this study, we further improved its composition to prevent oxidation, and have developed ClearSeeAlpha, which can be applied to a broader range of plant species and tissues. Sodium sulfite, one of the reductants, prevented brown pigmentation due to oxidation during clearing treatment. Using ClearSeeAlpha, we show that it is possible to obtain clear chrysanthemum leaves, tobacco and Torenia pistils and fertilized Arabidopsis thaliana fruits—tissues that have hitherto been challenging to clear. Moreover, we show that the fluorescence intensity of purified fluorescent proteins emitting light of various colors was unaffected in the ClearSeeAlpha solution; only the fluorescence intensity of TagRFP was reduced by about half. ClearSeeAlpha should be useful in the discovery and analysis of biological phenomena occurring deep inside the plant tissues.

    DOI： 10.1093/pcp/pcab033

    Web of Science

    Scopus

    PubMed

    Other Link： https://academic.oup.com/pcp/article-pdf/62/8/1302/41119229/pcab033.pdf

  12. Advances in Two-Photon Imaging in Plants Reviewed

    Yoko Mizuta

    Plant and Cell Physiology   Vol. 62 ( 8 ) page： 1224 - 1230   2021.8

    　More details

    Authorship：Lead author,　Last author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

    Abstract

    Live and deep imaging play a significant role in the physiological and biological study of organisms. Two-photon excitation microscopy (2PEM), also known as multiphoton excitation microscopy, is a fluorescent imaging technique that allows deep imaging of living tissues. Two-photon lasers use near-infrared (NIR) pulse lasers that are less invasive and permit deep tissue penetration. In this review, recent advances in two-photon imaging and their applications in plant studies are discussed. Compared to confocal microscopy, NIR 2PEM exhibits reduced plant-specific autofluorescence, thereby achieving greater depth and high-resolution imaging in plant tissues. Fluorescent proteins with long emission wavelengths, such as orange–red fluorescent proteins, are particularly suitable for two-photon live imaging in plants. Furthermore, deep- and high-resolution imaging was achieved using plant-specific clearing methods. In addition to imaging, optical cell manipulations can be performed using femtosecond pulsed lasers at the single cell or organelle level. Optical surgery and manipulation can reveal cellular communication during development. Advances in in vivo imaging using 2PEM will greatly benefit biological studies in plant sciences.

    DOI： 10.1093/pcp/pcab062

    Web of Science

    Scopus

    PubMed

    Other Link： https://academic.oup.com/pcp/article-pdf/62/8/1224/41119175/pcab062.pdf

  13. A Peptide Pair Coordinates Regular Ovule Initiation Patterns with Seed Number and Fruit Size Reviewed International journal

    Nozomi Kawamoto, Dunia Pino Del Carpio, Alexander Hofmann, Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama, Naoyuki Uchida, Keiko U. Torii, Lucia Colombo, Georg Groth, Rüdiger Simon

    Current Biology   Vol. 30 ( 22 ) page： 4352 - +   2020.11

    　More details

    Language：English   Publishing type：Research paper (scientific journal)   Publisher：Elsevier BV  

    Ovule development in Arabidopsis thaliana involves pattern formation, which ensures that ovules are regularly arranged in the pistils to reduce competition for nutrients and space. Mechanisms underlying pattern formation in plants, such as phyllotaxis, flower morphogenesis, or lateral root initiation, have been extensively studied, and genes controlling the initiation of ovules have been identified. However, the fundamental patterning mechanism that determines the spacing of ovule anlagen within the placenta remained unexplored. Using natural variation analysis combined with quantitative trait locus analysis, we found that the spacing of ovules in the developing gynoecium and fruits is controlled by two secreted peptides, EPFL2 and EPFL9 (also known as Stomagen), and their receptors from the ERECTA (ER) family that act from the carpel wall and the placental tissue. We found that a signaling pathway controlled by EPFL9 acting from the carpel wall through the LRR-receptor kinases ER, ERL1, and ERL2 promotes fruit growth. Regular spacing of ovules depends on EPFL2 expression in the carpel wall and in the inter-ovule spaces, where it acts through ERL1 and ERL2. Loss of EPFL2 signaling results in shorter gynoecia and fruits and irregular spacing of ovules or even ovule twinning. We propose that the EPFL2 signaling module evolved to control the initiation and regular, equidistant spacing of ovule primordia, which may serve to minimize competition between seeds or facilitate equal resource allocation. Together, EPFL2 and EPFL9 help to coordinate ovule patterning and thereby seed number with gynoecium and fruit growth through a set of shared receptors.

    DOI： 10.1016/j.cub.2020.08.050

    Web of Science

    Scopus

    PubMed

  14. Technologies for the understanding and control of biological phenomena in field-grown plants Invited Reviewed

    Takaki Yamauchi, Sota Fujii, Masanori Okamoto, Yu Tanaka, Yoko Mizuta, Kei Hiruma, Kentaro Yoshida, Eiji Yamamoto, Takayuki Ohnishi, Yoshiaki Inukai

    Breeding Research   Vol. 22 ( 1 ) page： 75 - 82   2020

    　More details

    Language：Japanese   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society of Breeding  

    DOI： 10.1270/jsbbr.22.w03

    CiNii Research

  15. Chemical signaling for pollen tube guidance at a glance Invited Reviewed

    Yoko Mizuta, Tetsuya Higashiyama

    Journal of Cell Science   Vol. 131 ( 2 ) page： jcs208447   2018.1

    　More details

    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)   Publisher：Company of Biologists Ltd  

    Pollen tube guidance is a unique navigating system that is required for the successful sexual reproduction of plants. As plant sperm cells are non-motile and egg cells are embedded deep inside the female tissues, a pollen tube delivers the two sperm cells that it contains by growing towards the ovule, in which the egg cell resides. Pollen tube growth towards the ovule is precisely controlled and divided into two stages, preovular and ovular guidance. In this Cell Science at a Glance article and accompanying poster, we provide a comprehensive overview of pollen tube guidance and highlight some of the attractant peptides used during ovular guidance. We further discuss the precise one-to-one guidance system that exists in multi-ovular plants. The pollen tube-blocking system, which is mediated by male-female crosstalk communication, to avoid attraction of multiple pollen tubes, is also reviewed.

    DOI： 10.1242/jcs.208447

    Web of Science

    Scopus

    PubMed

    CiNii Research

  16. Three-Dimensional Multiphoton Imaging of Transcription Factor by ClearSee. Reviewed

    Yoko Mizuta, Katsutoshi Tsuda

    Methods in molecular biology (Clifton, N.J.)   Vol. 1830   page： 257 - 268   2018

    　More details

    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/978-1-4939-8657-6_15

    Web of Science

    Scopus

    PubMed

  17. Plant tissue clearing for fluorescence imaging Invited

    Daisuke Kurihara, Yoko Mizuta

    Plant Morphology   Vol. 29 ( 1 ) page： 81-86   2017.4

    　More details

    Language：Japanese  

  18. Plant tissue clearing for fluorescence imaging Invited

    Daisuke Kurihara, Yoko Mizuta

    Plant Morphology   Vol. 29 ( 1 ) page： 81 - 86   2017.4

    　More details

    Authorship：Last author   Language：Japanese   Publisher：The Japanese Society of Plant Morphology  

    <p>Fluorescent proteins enable us to visualize not only single cells but also organelles and molecules. However, plants have opaque bodies, complicated tissue structures containing air spaces, and autofluorescent compounds, which hinder fluorescence imaging of plants without mechanical sectioning. Recently, various clearing techniques have been developed for plant tissues to reduce the mismatch of refractive index and to remove the colored components. In this review, we will describe various clearing techniques for fluorescence imaging in plants.</p>

    DOI： 10.5685/plmorphol.29.81

    CiNii Research

  19. On the Cover

    Mizuta Yoko, Kurihara Daisuke

    PLANT MORPHOLOGY   Vol. 29 ( 1 ) page： 0 - 0   2017

    　More details

    Language：Japanese   Publisher：The Japanese Society of Plant Morphology  

    DOI： 10.5685/plmorphol.29.0

    CiNii Research

  20. Visualization of plant sexual reproduction in the whole-mount pistil by Clearsee Reviewed

    Yoko Mizuta,Daisuke Kurihara, Tetsuya Higashiyama

    Cytologia   Vol. 81   page： １−２   2016.1

    　More details

    Authorship：Lead author,　Corresponding author   Language：English  

  21. ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging. Reviewed

    Daisuke Kurihara, Yoko Mizuta, Yoshikatsu Sato, Tetsuya Higashiyama

    Development   Vol. 142 ( 23 ) page： 4168-4179   2015.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1242/dev.127613

    PubMed

  22. Two-photon imaging with longer wavelength excitation in intact Arabidopsis tissues. Reviewed

    Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama

    Protoplasma   Vol. 252 ( 5 ) page： 1231-1240   2015.9

    　More details

    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1007/s00709-014-0754-5

    PubMed

  23. In vivo deep imaging and optical manipulation by two-photon excitation microscopy(<Feature Articles>Live-cell imaging) Invited

    Mizuta Yoko, Kurihara Daisuke

    Regulation of Plant Growth & Development   Vol. 49 ( 2 ) page： 96-103   2014.12

    　More details

    Authorship：Lead author   Language：Japanese  

  24. Antisense gene inhibition by phosphorothioate antisense oligonucleotide in Arabidopsis pollen tubes. Reviewed

    Yoko Mizuta, Tetsuya Higashiyama

    The Plant journal   Vol. 78 ( 3 ) page： 516-526   2014.5

    　More details

    Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1111/tpj.12461

    PubMed

  25. Growth assay of individual pollen tubes arrayed by microchannel device

    Mitsuhiro Horade, Naoki Yanagisawa, Yoko Mizuta, Tetsuya Higashiyama, Hideyuki Arata

    Microelectronic Engineering   Vol. 118   page： 25-28   2014.4

    　More details

    Language：English  

  26. 花粉管のin vivoイメージングで観えてきた植物生殖の実態—2光子顕微鏡を用いたアプローチ— Invited Reviewed

    水多陽子, 栗原大輔, 東山哲也

    PLANT MORPHOLOGY   Vol. 26 ( 1 ) page： 71   2014.4

    　More details

    Authorship：Lead author   Language：Japanese  

  27. In vivo live-cell imaging in plant tissues by two-photon excitation microscopy Invited

    Mizuta Yoko, Kurihara Daisuke, Higashiyama Tetsuya

    PLANT MORPHOLOGY   Vol. 26 ( 1 ) page： 25-30   2014

    　More details

    Authorship：Lead author,　Corresponding author   Language：Japanese  

  28. Plant-on-a-chip microfluidic-system for quantitative analysis of pollen tube guidance by signaling molecule: Towards cell-to-cell communication study Reviewed

    Mitsuhiro Horade, Yoko Mizuta, Noritada Kaji, Tetsuya Higashiyama, Hideyuki Arata

    Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012     page： 1027-1029   2012.1

    　More details

    Language：English  

  29. Rice expression atlas in reproductive development. Reviewed

    Fujita M, Horiuchi Y, Ueda Y, Mizuta Y, Kubo T, Yano K, Yamaki S, Tsuda K, Nagata T, Niihama M, Kato H, Kikuchi S, Hamada K, Mochizuki T, Ishimizu T, Iwai H, Tsutsumi N, Kurata N

    Plant & cell physiology   Vol. 51 ( 12 ) page： 2060-2081   2010.12

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcq165

    PubMed

  30. Rice pollen hybrid incompatibility caused by reciprocal gene loss of duplicated genes. Reviewed

    Yoko Mizuta, Yoshiaki Harushima, Nori Kurata

    Proceedings of the National Academy of Sciences of the United States of America   Vol. 107 ( 47 ) page： 20417-20422   2010.11

    　More details

    Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1073/pnas.1003124107

    PubMed

  31. Separated transcriptomes of male gametophyte and tapetum in rice: validity of a laser microdissection (LM) microarray. Reviewed

    Suwabe K, Suzuki G, Takahashi H, Shiono K, Endo M, Yano K, Fujita M, Masuko H, Saito H, Fujioka T, Kaneko F, Kazama T, Mizuta Y, Kawagishi-Kobayashi M, Tsutsumi N, Kurata N, Nakazono M, Watanabe M

    Plant & cell physiology   Vol. 49 ( 10 ) page： 1407-1416   2008.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcn124

    PubMed

  32. Various spatiotemporal expression profiles of anther-expressed genes in rice. Reviewed

    Hobo T, Suwabe K, Aya K, Suzuki G, Yano K, Ishimizu T, Fujita M, Kikuchi S, Hamada K, Miyano M, Fujioka T, Kaneko F, Kazama T, Mizuta Y, Takahashi H, Shiono K, Nakazono M, Tsutsumi N, Nagamura Y, Kurata N, Watanabe M, Matsuoka M

    Plant & cell physiology   Vol. 49 ( 10 ) page： 1417-1428   2008.10

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

    DOI： 10.1093/pcp/pcn128

    PubMed

  33. Morphological Characterization of Three Intergeneric Hybrids Among Gloriosa superba `Lutea', Littonia modesta, and Sandersonia aurantiaca (Colchicaceae) Reviewed

    Amano J, Kuwayama S, Mizuta Y, Nakano M, Godo T, Okuno H

    Hort. Science   Vol. 43   page： 115-118   2008

    　More details

    Language：English   Publishing type：Research paper (scientific journal)  

  34. Mapping of a pair of reproductive barrier loci observed in a cross between Nipponbare and Kasalath

    Yoko Mizuta, Yoshiaki Harushima, Nori Kurata

    Rice Genetics Newsletter   Vol. 23   page： 33-35   2007

    　More details

    Authorship：Lead author   Language：English  

  35. Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses

    Amano Junji, Kuwayama Sachiko, Mizuta Yoko, Oomiya Tomo, Nakamura Toru, Nakano Masaru

    Journal of the Japanese Society for Horticultural Science   Vol. 76 ( 1 ) page： 73-78   2007

    　More details

    Language：English  

▼display all

To the head of Papers.▲

Books 6

  1. ゲノム編集技術 ～実験上のポイント／産業利用に向けた研究開発動向と安全性周知

    水多陽子（ Role： Contributor ,  第3節 第1項 ゲノム編集酵素のデリバリーと植物の特性改良）

    株式会社 情報機構  2023.1  （ ISBN:9784865022421 ）

  2. エッセンシャル遺伝学・ゲノム科学 (原著第7版) 第9章 Reviewed

    植田 美那子, 栗原 大輔, 水多 陽子（ Role： Joint translator ,  第9章）

    化学同人  2021.2  （ ISBN:9784759820485 ）

    　More details

    Language：Japanese Book type：Textbook, survey, introduction

  3. Plant Transcription Factor

    Yoko Mizuta, Katsutoshi Tsuda, Yamaguchi Nobutoshi（ Role： Contributor ,  Three-dimensional multiphoton imaging of transcription factor by ClearSee.）

    Springer protocols  2018.7  （ ISBN:9781493986569 ）

    　More details

    Responsible for pages：257-268   Language：English Book type：Scholarly book

  4. THE SECRET LIFE OF PLANTS

    Daisuke Kurihara and Yoko Mizuta（ Role： Joint author）

    National geographic  2017.4 

    　More details

    Language：English Book type：Image

    Other Link： https://www.amazon.co.jp/National-Geographic-US-May-2017/dp/B072L2RDWM

  5. 植物の中まで丸見え

    栗原 大輔、水多 陽子（ Role： Joint author）

    ナショナルジオグラフィック　日本版  2016.6 

    　More details

  6. 植物の透明化

    東山 哲也、栗原 大輔、水多 陽子（ Role： Joint author）

    太田出版  2016.4  （ ISBN:9784778315160 ）

    　More details

    Language：Japanese Book type：Image

▼display all

To the head of Books.▲

MISC 4

  1. 植物細胞の分化運命の制御と可塑性 Reviewed

    丸山 大輔, 水多 陽子, 山岡 尚平

    植物科学の最前線   Vol. 14 ( A ) page： 1 - 2   2023

    　More details

    Language：Japanese   Publisher：公益社団法人 日本植物学会  

    DOI： 10.24480/bsj-review.14a1.00236

    CiNii Research

  2. 特集「異分野融合が推進するイメージング研究」

    水多陽子、多喜正泰

    Plant Morphology   Vol. 33   page： 1 - 2   2021.5

    　More details

    Authorship：Lead author,　Corresponding author   Language：Japanese  

  3. Plant imaging research promoted by interdisciplinary collaborations

    Mizuta Yoko, Taki Masayasu

    PLANT MORPHOLOGY   Vol. 33 ( 1 ) page： 1 - 2   2021

    　More details

    Language：Japanese   Publisher：The Japanese Society of Plant Morphology  

    <p>In recent decades, various interdisciplinary technologies promote plant imaging research, such as MEMS (Micro-Electro-Mechanical-Systems), organic synthesis, genomics, and mathematical modeling. We have organized a symposium entitled ‘Plant imaging research promoted by interdisciplinary collaborations’ at the 84th Annual Meeting of the Botanical Society of Japan on September 21<sup>st</sup>, 2020. This symposium was co-sponsored by The Birth of New Plant Species (Scientific Research on Innovative Areas, a MEXT Grant-in-Aid Project), Integrated Imaging Research Support (IIRS), and The Japanese Society of Plant Morphology. We had five young expert researchers’ presentations to introduce their innovative research with cutting-edge techniques for imaging. Current issues in the plant research, their solutions by the new imaging technologies, and plant imaging potential were interactively discussed involving speakers and audiences. This symposium was held using the online conference tool ‘Zoom’ under the first trial of the online Annual Meeting of the Botanical Society of Japan due to the influence of the COVID-19.</p>

    DOI： 10.5685/plmorphol.33.1

    CiNii Research

  4. 卓上型走査型電子顕微鏡によるシロイヌナズナめしべの観察像

    水多 陽子, 栗原 大輔

    ＰＬＡＮＴ　ＭＯＲＰＨＯＬＯＧＹ   Vol. 29 ( 1 ) page： 0-0   2017

    　More details

    Authorship：Lead author   Language：Japanese  

    <p>卓上型走査型電子顕微鏡によるシロイヌナズナめしべの観察像 めしべの片側の子房壁を取り除いて，キーエンスVHX-2000 およびVHX-D510 により撮影．</p>

    DOI： 10.5685/plmorphol.29.0

To the head of MISC.▲

Presentations 87

  1. 花粉発生過程のライブイメージングと一過的導入による分裂誘導

    永原 史織, 丸山 大輔, 山岡 尚平, 水多 陽子

    日本植物学会第88回大会  2024.9.16 

    　More details

    Event date： 2024.9

    Presentation type：Poster presentation  

  2. 花粉特異的ROPGEFsはC末モチーフを介したPRK6受容体との相互作用により花粉管の挙動を調節する

    内木 希美, 長江 拓也, 四方 明格, 海老根 一生, 水多 陽子, 東山 哲也, 武内 秀憲

    日本植物学会第88回大会  2024.9.16 

    　More details

    Event date： 2024.9

    Presentation type：Poster presentation  

  3. 花粉発生過程のライブイメージングと一過的導入による分裂誘導

    永原史織, 丸山大輔, 山岡尚平, 水多陽子

    日本植物形態学会第36回大会  2024.9.13 

    　More details

    Event date： 2024.9

    Presentation type：Poster presentation  

  4. 青色光照射による効率的な花粉管破裂誘導法の開発

    杉直也, 須崎大地, 水多陽子, 木下哲, 丸山大輔

    日本植物生理学会第65回年会  2024.3.18 

    　More details

    Event date： 2024.3

    Presentation type：Oral presentation (general)  

  5. 花粉発生過程のライブイメージングと分裂誘導による雄原細胞の分化機構の解明

    永原史織, 丸山大輔, 山岡尚平, 水多陽子

    日本植物生理学会第65回年会  2024.3.19 

    　More details

    Event date： 2024.3

    Presentation type：Oral presentation (general)  

  6. 新規モデル植物のゲノムを"編集する・解析する"I 「一過的な遺伝子導入による植物生殖細胞のゲノム編集と遺伝子発現制御」 Invited

    水多陽子

    基礎生物学研究所 新規モデル生物開発トレーニングコース  2024.3.5  基礎生物学研究所　超階層生物学センター・新規モデル生物開発室

    　More details

    Event date： 2024.3

    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

    Venue：基礎生物学研究所  

  7. 新規モデル植物のゲノムを"編集する・解析する"I 「一過的な遺伝子導入による植物生殖細胞のゲノム編集と遺伝子発現制御」 Invited

    水多陽子

    基礎生物学研究所 新規モデル生物開発トレーニングコース  2024.3.5 

    　More details

    Event date： 2024.3

    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  8. 二光子イメージングによる一対一の花粉管誘引ダイナミクスと多花粉管拒否機構の解析

    水多 陽子, 榊原 大悟, 永原 史織, 金城 行真, 長江 拓也, 栗原 大輔, 東山 哲也

    日本植物学会第87回大会  2023.9.7 

    　More details

    Event date： 2023.9

    Presentation type：Oral presentation (general)  

  9. 助細胞における花粉管誘引シグナルの極性分泌ダイナミクスの解析

    永原 史織, 丸山 大輔, 東山 哲也, 水多 陽子, 武内 秀憲

    日本植物学会第87回大会  2023.9.7 

    　More details

    Event date： 2023.9

    Presentation type：Oral presentation (general)  

  10. Half-valve法で明らかになった多段階の多花粉管拒否メカニズム

    水多陽子, 榊原大悟, 永原史織, 金城行真, 長江拓也, 栗原大輔, 東山哲也

    日本植物形態学会第35回大会  2023.9.6 

    　More details

    Event date： 2023.9

    Presentation type：Poster presentation  

  11. アブラナ科植物の花粉管誘導過程における異種と同種を見分ける認証機構の解明

    長江 拓也, 武内 秀憲, 永原 史織, 水多 陽子, 東山 哲也

    日本植物学会第87回大会  2023.9.4 

    　More details

    Event date： 2023.9

    Presentation type：Poster presentation  

  12. ライブイメージングで解明するコムギ配偶子致死 遺伝子Gc2の作用機構

    角井 宏行, 村田 和樹, 内野 智樹, 佐藤 良勝, 水多 陽子, 那須田 周平

    日本育種学会第143回講演会  2023.3.17 

    　More details

    Event date： 2023.3

    Presentation type：Oral presentation (general)  

  13. 花粉によるゲノム編集酵素のデリバリーと周辺技術の開発 Invited

    水多陽子, 皆川吉, 田中左恵子, 江面浩

    第64回日本植物生理学会年会  2023.3.15 

    　More details

    Event date： 2023.3

    Presentation type：Symposium, workshop panel (nominated)  

  14. コムギ配偶子致死遺伝子Gcの機能解析 Invited

    角井宏行, 村田和樹, 佐藤良勝, 水多陽子, 那須田周平

    第17回ムギ類研究会  2022.12.17 

    　More details

    Event date： 2022.12

    Presentation type：Oral presentation (general)  

  15. 花粉を用いた植物生殖細胞のゲノム編集と周辺技術の開発 Invited

    水多 陽子

    2022年度植物科学シンポジウム「植物科学で挑む、社会実装への道」  2022.12.6 

    　More details

    Event date： 2022.12

    Presentation type：Symposium, workshop panel (nominated)  

  16. イメージングで解き明かす花粉の一生と受精メカニズム Invited

    水多陽子

    京大植物縦横無尽の会  2022.10.21 

    　More details

    Event date： 2022.10

    Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  17. 雌しべの中の道標を辿る花粉管の旅：被子植物が獲得したPRKファミリー受容体の機能

    武内秀憲, 別所-上原奏子, 長江拓也, 井本美紀, 内木希美, 永原史織, 水多陽子, 東山哲也

    日本植物学会第86回大会  2022.9.19 

    　More details

    Event date： 2022.9

    Presentation type：Symposium, workshop panel (nominated)  

  18. アブラナ科植物の花粉管誘導過程における異種と同種を見分ける認証機構の解析

    長江拓也, 武内秀憲, 水多陽子, 東山哲也

    日本植物学会第86回大会  2022.9.19 

    　More details

    Event date： 2022.9

    Presentation type：Oral presentation (general)  

  19. 一過的発現による花粉の分化運命の制御

    水多陽子

    日本植物学会第86回大会  2022.9.17 

    　More details

    Event date： 2022.9

    Presentation type：Symposium, workshop panel (nominated)  

  20. 花粉のゲノム編集とRNP導入

    皆川 吉, 田中 左恵子, 大島 崇彰, 水多 陽子, 東山 哲也, 江面 浩, 間 和彦

    第39回日本植物バイオテクノロジー学会大会  2022.9.13 

    　More details

    Event date： 2022.9

    Presentation type：Oral presentation (general)  

  21. 花粉を用いた植物生殖細胞のゲノム編集 Invited

    水多陽子

    日本ゲノム編集学会第7回大会, シンポジウム「ゲノム編集植物の科学」  2022.6.8 

    　More details

    Event date： 2022.6

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  22. 二光子顕微鏡を用いて生殖過程を定量的に捉え、異種と同種を見分ける認証機構に迫る

    長江 拓也, 武内 秀憲, 水多陽子, 東山 哲也

    第44回日本分子生物学会年会  2021.12.1 

    　More details

    Event date： 2021.12

  23. イネにおける成長相転換とジベレリン生合成の時空間的解析

    (12) 新美陽子, 永井啓祐, 保浦徳昇, 水多陽子, 竹林 裕美子, 小嶋美紀子, 榊原均, 島谷善平, 寺田理枝, 辻寛之, 芦苅基行

    日本育種学会第140回講演会 

    　More details

    Event date： 2021.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Venue：弘前大学  

  24. 花粉管を用いた生殖細胞のゲノム編集と導入細胞の可視化

    永原史織, 東山哲也, 水多陽子

    日本植物学会第85回大会  2021.9.9 

    　More details

    Event date： 2021.9

    Language：Japanese   Presentation type：Poster presentation  

    Venue：東京都立大学  

  25. Genetic modification of plant germ cells by using delivery of genome-editing tools via pollen tubes Invited

    Yoko Mizuta

    The 38th Annnual Meeting of The Japanese Society for Plant Biotechnology – Made in Japan: The frontiers of genome editing technology development from japan, driving the world forward  2021.9.11 

    　More details

    Event date： 2021.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  26. 花粉管発生におけるライブイメージングと生殖細胞の改変

    水多陽子

    第62回日本植物生理学会年会・関連集会「植物生殖改変ワークショップ」  2021.3.14 

    　More details

    Event date： 2021.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

  27. イネ花粉成熟期における糖化機構の解明

    (9) 葉山旺，河合恭甫，杉原諒彦，森井南美，竹原清日，水多陽子，上口美弥子

    第62回日本植物生理学会年会  2021.3.14 

    　More details

    Event date： 2021.3

    Language：Japanese   Presentation type：Poster presentation  

    Venue：島根大学  

  28. 観る、知る、利用する。植物の花に秘められたいのちのしくみ Invited

    水多陽子

    第7回名古屋大学の卓越・先端・次世代 研究シンポジウム「学問の意義と貢献：研究のインパクトと社会との対話」  2021.1.22 

    　More details

    Event date： 2021.1

    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  29. 極彩色で観る 植物がいのちをつなぐしくみ Invited

    水多陽子

    サイエンスカフェ（KMI x ITbM Mix Cafe）集まれ！話そう！科学のワクワク！  2020.9.30 

    　More details

    Event date： 2020.9

    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  30. 花粉不発芽を引き起こすDPL遺伝子の機能解析

    水多陽子, 栗原大輔

    日本植物学会第84回大会  2020.9.19  日本植物学会

    　More details

    Event date： 2020.9

    Language：Japanese   Presentation type：Oral presentation (general)  

  31. Ca2+に着目した花粉管の破裂制御における種認証機構の解析

    五郎丸輝明, 長江拓也, 水多陽子, 東山哲也

    日本植物学会第84回大会  2020.9.21 

    　More details

    Event date： 2020.9

    Language：Japanese   Presentation type：Poster presentation  

  32. 花粉管誘引において同種認証機構を担う雌しべ組織の検討

    長江拓也, 武内秀憲, 水多陽子, 須崎大地, 東山哲也

    日本植物学会第84回大会  2020.9.21 

    　More details

    Event date： 2020.9

    Language：Japanese   Presentation type：Poster presentation  

  33. コロナ禍における植物研究者の変容と進化 Invited

    水多陽子

    第1回 名古屋大学高等研究院ウェビナー（高等研究院×未来社会創造機構） 「未来を見据えるコロナ禍の研究者たち」  2020.6.2 

    　More details

    Event date： 2020.6

    Language：Japanese   Presentation type：Public lecture, seminar, tutorial, course, or other speech  

  34. 花粉管をベクターとしたゲノム編集酵素のデリバリー Invited

    水多陽子

    プロジェクト横断型公開シンポジウム「植物のゲノム編集基盤技術開発の現状と展望」 

    　More details

    Event date： 2020.2

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Country：Japan  

  35. 「花のなかを観る」植物のタネができるしくみ Invited

    水多陽子

    次世代研究者シンポジウム2019  2019.10.31 

    　More details

    Event date： 2019.10

    Language：English   Presentation type：Oral presentation (general)  

  36. 花粉管破裂制御におけるCa2+動態と種認証機構の関係

    五郎丸輝明，東山哲也，水多陽子，長江拓也

    日本植物学会第83回大会 

    　More details

    Event date： 2019.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  37. 深部イメージングで探る植物生殖の謎

    水多陽子, 栗原大輔, 東山 哲也

    日本植物学会第83回大会  

    　More details

    Event date： 2019.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  38. 二光子イメージングによる一対一受精機構の解明

    水多 陽子, 栗原 大輔, 東山 哲也

    日本植物形態学会第31回大会 

    　More details

    Event date： 2019.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  39. 花粉管をベクターとした生殖細胞の遺伝子改変 Invited

    水多陽子、永原史織、東山哲也

    日本育種学会第136回講演会 

    　More details

    Event date： 2019.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Country：Japan  

  40. 花粉への一過的遺伝子導入系による花粉管および精細胞の動態解析

    永原史織, 栗原大輔, 東山哲也, 水多陽子

    第60回日本植物生理学会年会  2019.3.14 

    　More details

    Event date： 2019.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  41. 花粉管をベクターとした生殖細胞の遺伝子改変と植物生殖機構の解明

    水多陽子, 永原史織, 栗原大輔, 東山哲也

    第60回日本植物生理学会年会 シンポジウム  2019.3.13 

    　More details

    Event date： 2019.3

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Country：Japan  

  42. 二光子励起顕微鏡で解く植物生殖の謎

    水多陽子

    第7回 植物イメージングの会  2019.2.22 

    　More details

    Event date： 2019.2

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Country：Japan  

  43. 花粉のゲノム編集

    水多陽子, 永原史織, 東山哲也

    日本植物学会第82回大会  2018.9.16 

    　More details

    Event date： 2018.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  44. 植物透明化試薬ClearSeeの改良

    栗原大輔, 水多陽子, 永原史織, 東山哲也

    日本植物学会第82回大会  2018.9.15 

    　More details

    Event date： 2018.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  45. 花粉のゲノム編集法

    水多陽子, 永原史織, 東山哲也

    日本植物形態学会第30回大会  2018.9.13 

    　More details

    Event date： 2018.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  46. さらなる植物透明化に向けて

    栗原大輔, 水多陽子, 永原史織, 東山哲也

    日本植物形態学会第30回大会  2018.9.13 

    　More details

    Event date： 2018.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  47. Two-photon imaging reveals spatio-temporal regulation on the one-to-one pollen tube guidance International conference

    Yoko Mizuta, Daisuke Kurihara, Shiori Nagahara, Tetsuya Higashiyama

    The 25th International Congress on Sexual Plant Reproduction (ICSPR2018)  2018.6.12 

    　More details

    Event date： 2018.6

    Language：English   Presentation type：Poster presentation  

    Country：Japan  

  48. パーティクルボンバードメント法による植物生殖細胞へのCRISPR/Cas9の導入

    永原 史織, 栗原 大輔, 東山 哲也, 水多 陽子

    2017年度生命科学系学会合同年次大会（ConBio2017）  2017.12.9 

    　More details

    Event date： 2017.12

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  49. In vivo deep imaging revealed the mystery of pollen tube guidance Invited

    Yoko Mizuta

    Consortium of Biological Sciences 2017 (ConBio2017) – Frontiers in Reproductive Science –  2017.12.9 

    　More details

    Event date： 2017.12

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Country：Japan  

  50. Deeper, more detailed, more beautiful – Deep imaging inside the living plants – Invited

    Yoko Mizuta

    The 2nd Frontier Bioimaging Conference  2017.11.7 

    　More details

    Event date： 2017.11

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Country：Japan  

  51. 3Dプリンタを用いた植物組織の新しい3D画像データ提示手法の開発

    小笠原希実, 水多陽子, 東山哲也

    第58回 日本植物生理学会年会  2017.3.17 

    　More details

    Event date： 2017.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  52. ClearSee: a rapid optical clearing reagent for whole-plant fluorescence imaging.

    Daisuke Kurihara, Yoko Mizuta, Yoshikatsu Sato, Tetsuya Higashiyama

    The 1st ABiS Symposium -Towards the Future of Advanced Bioimaging for Life Sciences-  2017.2.19 

    　More details

    Event date： 2017.2

    Presentation type：Symposium, workshop panel (nominated)  

  53. 植物深部に侵入した微生物を光学顕微鏡で観察する

    栗原大輔, 大津美奈, 水多陽子, 東山哲也

    日本植物学会 第80回大会 

    　More details

    Event date： 2016.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Country：Japan  

  54. ClearSee で観る植物生殖のメカニズム

    水多陽子, 栗原大輔, 東山哲也

    日本植物学会 第80回大会 

    　More details

    Event date： 2016.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  55. Live imaging of membrane traffic in Arabidopsis pollen tubes

    Live imaging of membrane traffic in Arabidopsis pollen tubes 

    　More details

    Event date： 2016.6

    Presentation type：Poster presentation  

    Country：Japan  

  56. 花粉管と胚珠の相互作用に関する シミュレーション解析 International conference

    鈴木孝征、水多陽子、東山哲也

    第 57 回 日本植物生理学会年会 

    　More details

    Event date： 2016.3

    Presentation type：Oral presentation (general)  

    Country：Japan  

  57. 深部観察で Whole plant imaging を目指す

    栗原大輔、水多陽子、東山哲也

    日本植物学会第79回大会 

    　More details

    Event date： 2015.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Country：Japan  

  58. 一本の花粉管が一つの胚珠にたどりつくには？　〜深部イメージングで探る花粉管ガイダンスの謎〜 Invited

    水多陽子、栗原大輔、東山哲也

    日本植物学会第79回大会 

    　More details

    Event date： 2015.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Country：Japan  

  59. Two-photon imaging of pollen tube guidance in the Arabidopsis pistil

    Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama

    NIG Biological Symposium 

    　More details

    Event date： 2015.2

    Language：English  

    Country：Japan  

  60. ２光子顕微鏡を用いた花粉管ガイダンスのin vivoライブイメージング

    水多陽子、栗原大輔、東山哲也

    日本植物学会第78回大会 

    　More details

    Event date： 2014.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  61. 生体深部イメージングによる花粉管ガイダンスの「形」と「通り道」の解析

    水多陽子、栗原大輔、東山哲也

    日本植物形態学会第26回大会 

    　More details

    Event date： 2014.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  62. Deep imaging of pollen tube guidance by two-photon microscopy International conference

    Yoko Mizuta

    Deep imaging of pollen tube guidance by two-photon microscopy 

    　More details

    Event date： 2014.7

    Language：English   Presentation type：Symposium, workshop panel (nominated)  

    Country：Japan  

  63. Two-photon imaging of pollen tube growth and guidance in the pistil International conference

    Y Mizuta, D Kurihara, T Higashiyama

    23rd ICSPR: International Conference on Sexual Plant Reproduction 

    　More details

    Event date： 2014.7

    Language：English   Presentation type：Poster presentation  

    Country：Portugal  

  64. 2光子顕微鏡を用いた花粉管の受精競争と多精拒否の in vivo ライブイメージング

    水多陽子、栗原大輔、東山哲也

    動植物に共通する アロ認証機構の解明　第8回領域会議 

    　More details

    Event date： 2014.1

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  65. 2 光子顕微鏡による深部イメージングで観えてきた植物生殖の実態 Invited

    水多陽子、栗原大輔、東山哲也

    日本植物学会第77回大会 

    　More details

    Event date： 2013.9

    Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

    Country：Japan  

  66. 花粉管のin vivoイメージングで観えてきた植物生殖の実態　-2光子顕微鏡を用いたアプローチ-

    水多陽子、栗原大輔、東山哲也

    日本植物形態学会第 25 回大会 

    　More details

    Event date： 2013.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  67. 植物生殖プロセスにおける花粉管ガイダンスのオンチップ定量解析

    佐藤良勝, 洞出光洋, 水多陽子, 加地範匡, 東山哲也, 新田英之

    日本分析化学会年会講演要旨集  2013.8.27 

    　More details

    Event date： 2013.8

    Language：Japanese   Presentation type：Oral presentation (general)  

  68. Phosphorothioate antisense oligodeoxynucleotides to transiently supress gene expression in living pollen tubes International conference

    Y Mizuta, T Higashiyama

    24th International Conference on Arabidopsis Research (ICAR 2013) 

    　More details

    Event date： 2013.6

    Language：English   Presentation type：Poster presentation  

    Country：Australia  

  69. マイクロ流路を用いた花粉管細胞配列アッセイ

    洞出光洋, 水多陽子, 東山哲也, 新田英之

    化学とマイクロ・ナノシステム学会研究会講演要旨集  2013.5.23 

    　More details

    Event date： 2013.5

    Language：Japanese   Presentation type：Oral presentation (general)  

  70. マイクロ流路デバイスを用いた花粉管誘引物質の1分子ライブイメージング

    水多陽子, 洞出光洋, 後藤宏旭, 加地範匡, 新田英之, 東山哲也

    日本植物学会第76回大会 

    　More details

    Event date： 2012.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  71. 花粉管ガイダンスの分子実体解明に向けてーマイクロ流体デバイスを用いた試み

    水多陽子, 洞出光洋, 後藤宏旭, 加地範匡, 新田英之, 東山哲也

    日本植物形態学会第24回大会 

    　More details

    Event date： 2012.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  72. PDMSマイクロチャネルを用いた花粉管細胞伸長の計測と制御

    洞出光洋, 水多陽子, 加地範匡, 新田英之, 東山哲也

    化学とマイクロ・ナノシステム研究会講演要旨集  2012.5.17 

    　More details

    Event date： 2012.5

    Language：Japanese   Presentation type：Oral presentation (general)  

  73. 花粉管内標的遺伝子の機能阻害と花粉管誘引アッセイに対する新アプローチ

    水多陽子, 洞出光洋, 加地範匡, 後藤宏旭, 新田英之, 東山哲也

    第53回日本植物生理学会年会 

    　More details

    Event date： 2012.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  74. イネの生殖的隔離およびシロイヌナズナの生殖に関わる花粉側因子の同定

    水多陽子, 東山哲也, 春島嘉章, 倉田のり

    日本植物学会第75回大会 

    　More details

    Event date： 2011.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  75. S化オリゴを用いた花粉内標的遺伝子の機能阻害と受精に関わる花粉側因子の探索

    水多陽子, 東山哲也

    日本植物形態学会第23回大会 

    　More details

    Event date： 2011.9

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  76. イネ亜種間交雑で生殖的隔離を引き起こす重複遺伝子DPL1, 2の解析 Invited

    水多　陽子

    日本遺伝学会第82回大会 

    　More details

    Event date： 2010.9

    Language：Japanese  

    Country：Japan  

  77. イネ亜種間交雑で生殖的隔離を引き起こすDOPPELGANGER(DPL)1とDPL2の解析

    水多陽子, 春島嘉章, 倉田のり

    日本育種学会第117回講演会 

    　More details

    Event date： 2010.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  78. イネ亜種間交雑で生殖的隔離を引き起こすDOPPELGANGER(DPL)1およびDPL2遺伝子の解析

    水多陽子, 春島嘉章, 倉田のり

    第51回日本植物生理学会年会 

    　More details

    Event date： 2010.3

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  79. Analysis of a pair of genes, DOPPELGANGER(DPL)1 and DPL2 responsible for reproductive isolation between two rice subspecies International conference

    Yoko Mizuta, Yoshiaki Harushima, Nori Kurata

    International Symposium of Cell-Cell Communication in Plant Reproduction : from pollination to fertilization 

    　More details

    Event date： 2010.3

    Language：English   Presentation type：Poster presentation  

    Country：Japan  

  80. イネ亜種間交雑で生殖的隔離を引き起こすDOPPELGANGER1(DPL1)とDPL2の単離・解析

    水多陽子, 春島嘉章, 倉田のり

    第32回日本分子生物学会年会 

    　More details

    Event date： 2009.12

    Language：Japanese   Presentation type：Poster presentation  

    Country：Japan  

  81. イネ亜種間交雑において生殖的隔離を引き起こす相互作用遺伝子座DOPPELGANGER1と2の単離・解析

    水多陽子, 春島嘉章, 倉田のり

    日本遺伝学会第81回大会 

    　More details

    Event date： 2009.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  82. イネ亜種間交雑で生殖的隔離障壁となる重複遺伝子の解析

    水多陽子, 春島嘉章, 倉田のり

    日本育種学会114回講演会 

    　More details

    Event date： 2008.10

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  83. Positional Cloning of a Pair of Interactive Genes Causing Reproductive Barrier in the Hybrid Pollen of Rice International conference

    Yoko Mizuta, Yoshiaki Harushima, Nori Kurata

    XX International congress of Genetics 

    　More details

    Event date： 2008.7

    Language：English   Presentation type：Poster presentation  

    Country：Germany  

  84. イネ雑種花粉で作用する生殖的隔離障壁遺伝子のポジショナルクローニング

    水多陽子, 春島嘉章, 倉田のり

    第112回育種学会 

    　More details

    Event date： 2007.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  85. イネ雑種花粉で相互作用する2遺伝子座に起因する生殖的隔離

    水多陽子, 春島嘉章, 倉田のり

    日本遺伝学会第79回大会 

    　More details

    Event date： 2007.9

    Language：Japanese   Presentation type：Oral presentation (general)  

    Country：Japan  

  86. Production of interspecific and intergeneric hybrids among Colchicaceous ornamentals via ovule culture : 6. Characterization of intergeneric hybrid plants of Sandersonia aurantiaca × Gloriosa superba 'Lutea'

    AMANO J, KUWAYAMA S, SUGAWARA S, MIZUTA Y, GODO T, NAKANO M

    園芸学研究. 別冊, 園芸学会大会研究発表要旨  2007.3.24 

    　More details

    Event date： 2007.3

    Language：Japanese   Presentation type：Oral presentation (general)  

  87. Production of interspecific and intergeneric hybrids among Colchicaceous ornamentals via ovule culture : 4. Characterization of plants derived from Littonia modesta × Sandersonia aurantiaca

    KUWAYAMA S, AMANO J, SUGAWARA S, NAKAMURA T, MIZUTA Y, OOMIYA T, NAKANO M

    園芸学会雑誌. 別冊, 園芸学会大会研究発表  2005.10.1 

    　More details

    Event date： 2005.10

    Language：Japanese   Presentation type：Oral presentation (general)  

▼display all

To the head of Presentations.▲

Works 7

  1. National Geographic Magazine

    Daisuke Kurihara, Yoko Mizuta

    2017.4

    　More details

    Work type：Artistic work  

  2. The Secret life of Plants, National Geographic Magazine, May 2017

    Daisuke Kurihara, Yoko Mizuta

    2016.7

    　More details

    Work type：Artistic work  

  3. 植物の透明化（ケトル Vol.30）

    東山哲也

    2016.4

    　More details

    Work type：Artistic work   Location：ケトル Vol.30（太田出版）135ページ  

  4. めしべを丸ごと透明化（科学技術の美パネル展）

    水多 陽子

    2016

    　More details

    Work type：Artistic work   Location：科学技術の美パネル展  

    File： めしべを丸ごと透明化（科学技術の美パネル展）.pdf

  5. Where the Magic Happens (Cell Picture Show)

    Yoko Mizuta, Daisuke Kurihara, Tetsuya Higashiyama

    2015

    　More details

    Work type：Artistic work   Location：Cell Picture Show: The Biology of Sex  

    File： Where the Magic Happens (Cell Picture Show).pdf

  6. ロゴマーク ユキツバキ（日本植物学会第79回大会）

    水多 陽子

    2015

    　More details

    Work type：Artistic work   Location：日本植物学会第79回大会  

    File： ロゴマーク ユキツバキ（日本植物学会第79回大会）.pdf

  7. 生きている花粉管の花火（科学技術の美パネル展）

    水多 陽子

    2014

    　More details

    Work type：Artistic work   Location：科学技術の美パネル展  

    File： 生きている花粉管の花火（科学技術の美パネル展） .pdf

▼display all

To the head of Works.▲

KAKENHI (Grants-in-Aid for Scientific Research) 22

  1. Mechanisms of asymmetric division and differentiation during pollen development

    2024.10 - 2031.9

    Japan Science and Technology Agency (JST)  Fusion Oriented Research for disruptive Science and Technology (FOREST) 

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\26000000 （ Direct Cost: \20000000 、 Indirect Cost：\6000000 ）

  2. 非対称分裂による花粉の発生と分化機構の解明

    Grant number：24K02031  2024.4 - 2027.3

    日本学術振興会  科学研究費助成事業  基盤研究(B)

    水多 陽子

    　 More details

    Authorship：Principal investigator 

    Grant amount：\18590000 （ Direct Cost: \14300000 、 Indirect Cost：\4290000 ）

  3. Research on the regulatory mechanism of pollen male germ cell differentiation

    Grant number：2024-10  2024.4 - 2026.3

    The Asahi Glass Foundation  Chemistry & Life Sciences, Research Encouragement Grants 

    Yoko Mizuta

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\3000000 （ Direct Cost: \3000000 ）

  4. 植物の一対一受精を制御する雌雄細胞間シグナル伝達機構の解明

    2023.11 - 2025.10

    公益財団法人 大隅基礎科学創成財団  公益財団法人 大隅基礎科学創成財団　第7期（2023年度）研究助成 

    水多陽子

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  5. 花粉管と胚珠の1対1誘引を制御する雌雄細胞間シグナル分子の解析

    2023.11 - 2024.11

    公益財団法人 住友財団  公益財団法人 住友財団 2023年度基礎科学研究助成 

    水多陽子

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

    Grant amount：\1500000 （ Direct Cost: \1500000 ）

  6. Development of electrical particle bombardment for in planta gene delivery in the non-model plants

    2023.6 - 2024.3

    Nagoya University  Grant in aid for a research project to strengthen interdepartmental research collaboration 

    Yoko Mizuta, Daisuke Ichihara

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  7. Autonomous de novo polarity determination mechanism in pollen

    Grant number：22K19328  2022.6 - 2024.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

    　 More details

    Authorship：Principal investigator 

    Grant amount：\6500000 （ Direct Cost: \5000000 、 Indirect Cost：\1500000 ）

  8. シロイヌナズナ花粉管の伸長および誘引に関するライブイメージング研究

    2022.4

    国立研究開発法人 科学技術振興機構（JST）  CREST 

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Competitive

  9. Visualization and regulation of plant hunger and uptake signals for root to shoot communication (PI: Ryo Tabata, Nagoya University)

    2022.4 - 2025.3

    The Program for Promoting the Enhancement of Research Universities (Nagoya University Program for Research Enhancement), Young Researcher Units for the Advancement of New and Undeveloped Fields (B3) 

    Yoko Mizuta, Shunsuke Oishi, Ryo Tabata

    　 More details

    Authorship：Principal investigator 

    Plants grow by absorbing nutrients from the soil using vascular that are stretched throughout plant bodies. However, when nutrients are scarce, “How do plants sense hunger and uptake nutrients ?” is still largely unknown. In this research unit, we will focus on the “Hunger signals,” which are signals that convey hunger from root to shoot, and the “Uptake signals,” which are signals that promote nutrient absorption from shoot to root, to clarify the molecular mechanism of flexible adaptation to nutrition deficiency stresses through communication between the roots and shoots. We aim to create a new academic discipline that analyzes and controls plant stress on an unprecedented cross-sectional scale, from molecules to individuals, and from individuals to environment.

  10. UK-Japan joint research to elucidate the birth of plant sex chromosomes and the evolution of sex-determining systems

    Grant number：21KK0128  2021.10 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

    　 More details

    Authorship：Coinvestigator(s) 

  11. イメージングと遺伝子発現を基盤とした植物の受精に必須な遺伝子の解析

    2020.12 - 2022.3

    公益財団法人 木下記念事業団  公益財団法人 木下記念事業団 学術研究活動助成事業 

    水多陽子

    　 More details

    Authorship：Principal investigator 

  12. Remodeling plant reproduction system by cell fate manipulations.

    Grant number：20H05778  2020.10 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (B)

    Maruyama Daisuke

    　 More details

    Authorship：Coinvestigator(s)  Grant type：Competitive

    In plant reproduction, haploid tissues or gametophytes produce gametes after rounds of cell/nuclear proliferation and differentiation of gamete progenitor cells. Interestingly, several evidences have supported the plasticity or flexibility of cell fate in male and female gametophytic cells. Recent studies have begun to identify key factors regulating the identities of gametophytic cells. In this research project, we examined the mechanisms of the determination or conversion of cell fates in reproductive cells, via ectopic expression or mutations of the key regulatory factors. As a result, we could show representatives of "Remodeling of plant reproduction", through a production of anuclear pollen tubes. Establishing new protocols of cell fate manipulation, as well as preparation of new and unique plant materials, our approach will become the first step to uncover the principles of reproduction and possibly contribute plant breeding in the future.

  13. Molecular mechanism of cell fate and fertility determination of pollen sperm cells by single-cell tracking

    Grant number：20H05779  2020.10 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Transformative Research Areas (B)

    Mizuta Yoko

    　 More details

    Authorship：Principal investigator 

    Grant amount：\47710000 （ Direct Cost: \36700000 、 Indirect Cost：\11010000 ）

    In this study, some Nicotiana benthamiana fluorescent marker lines were developed. We established a method to observe and analyze pollen and pollen tube development using confocal microscopy with flourescent markers for a long period. We also transiently introduced genes into pollen and evaluated their effects on gene function and phenotype. Our results indicate that the position of the pollen nucleus at the first division is important for the differentiation of generative cell and vegetative cell. Gene-introduced pollen grains were isolated and gene expression analysis from a small number of gene-introduced pollen grains was performed by RNA-seq analysis. Furthermore, a method for automated isolation of transiently gene-introduced pollen was established. Pollination of the isolated pollen was used to evaluate pollen tube germination and following aspects. Some of the results of this research were reported in several articles and at conferences.

  14. Whole-Cell Dynamics of Membrane Traffic Driving Chemotropism（Tetsuya Higashiyama, Research Director）

    2020 - 2025

    JST CREST 

    　 More details

    Authorship：Coinvestigator(s) 

  15. 花粉による新植物育種技術の開発

    Grant number：JPMJTR194H  2019.10 - 2023.3

    国立研究開発法人 科学技術振興機構（JST）  研究成果展開事業 A-STEP 産学共同フェーズ（シーズ育成タイプ） 

    皆川吉、水多陽子

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  16. RNPを導入した花粉による新育種技術の開発

    Grant number：AS3011910U  2018.10 - 2019.9

    国立研究開発法人 科学技術振興機構（JST）  研究成果展開事業 A-STEP 産学共同フェーズ（シーズ育成タイプFS） 

    皆川吉、水多陽子

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  17. 雌雄のクロストークによる花粉管誘引のON/OFFスイッチの解明

    Grant number：18K14741  2018.4 - 2020.3

    日本学術振興会  科学研究費助成事業 若手研究 (B)  若手研究(B)

    水多 陽子

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  18. 花粉管をベクターとした遺伝子改変技術の開発

    Grant number：JPMJPR15QC  2016.4 - 2019.3

    国立研究開発法人 科学技術振興機構（JST）  JST さきがけ 

    水多陽子

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  19. 一分子ライブイメージングによる花粉管誘引ペプチドと花粉管の相互作用の解析

    2015.4 - 2017.3

    公益財団法人　旭硝子財団  公益財団法人　旭硝子財団　研究助成　第1分野 (化学・生命科学系) 奨励研究 

    水多陽子

    　 More details

    Authorship：Principal investigator 

  20. In vivoカルシウムイメージングによる雌雄細胞間の情報伝達機構の解析

    2015.4 - 2016.3

    光科学技術研究振興財団  光科学技術研究振興財団 研究助成（第二課題） 

    水多陽子

    　 More details

    Authorship：Principal investigator  Grant type：Competitive

  21. In vivoライブイメージングと遺伝学の融合による植物受精機構の動的理解

    Grant number：26840104  2014.4 - 2016.3

    科学研究費補助金  若手研究(B)

    　 More details

    Authorship：Principal investigator 

  22. イネ亜種間交雑における生殖的隔離障壁因子の単離及び機能解析

    Grant number：18075009  2007.4 - 2010.3

    科学研究費補助金 

    　 More details

    Authorship：Principal investigator 

▼display all

To the head of KAKENHI (Grants-in-Aid for Scientific Research).▲

Industrial property rights 8

  1. Method of introducing substances into the generative cell

    Sachi Minagawa, Saeko Tanaka, Yoko Kurihara (Mizuta), Yusaku Wada, Hiroshi Ezura, Kang Seung Won, Akase Kosuke, Worarad Kanjana, Mitalo Oscar Witere

    　More details

    Application no：特願2023-057717 (PCT/JP2024/012620)  Date applied：2023.3

  2. 対象細胞の濃縮方法

    栗原（水多）陽子、金城行真、東山哲也、他2名

    　More details

    Applicant：国立大学法人東海国立大学機構、他3者

    Application no：特願2022-023027  Date applied：2022.2

  3. Target cell enritchment methods

    Yoko Kurihara (Mizuta), Ikuma Kaneshiro, Tetsuya Higashiyama, Sachi Minagawa, Yusaku Wada

    　More details

    Application no：特願2022-023027  Date applied：2022.2

  4. パーティクルボンバードメント法において用いられる、細胞内へ導入される物質で被覆された微粒子が担持された担体の製造方法

    皆川吉、栗原（水多）陽子

    　More details

    Applicant：株式会社ニップン、国立大学法人東海国立大学機構、国立大学法人　筑波大学、株式会社ファスマック

    Date applied：2020.2

    Announcement no：2021-132547  Date announced：2021.9

    Country of applicant：Domestic   Country of acquisition：Domestic

  5. A method for producing carriers bearing fine particles coated with substances to be introduced into cells for use in the particle bombardment

    Sachi Minagawa, Yoko Kurihara (Mizuta)

    　More details

    Application no：特願2020-029814  Date applied：2020.2

    Announcement no：特開2021-132547  Date announced：2021.9

  6. 花粉への物質導入方法

    栗原（水多）陽子, 皆川吉, 永原史織

    　More details

    Date applied：2019.4

    Announcement no：2020-171262  Date announced：2020.10

    Country of applicant：Domestic   Country of acquisition：Domestic

  7. A methods of introducing substances into pollen

    Yoko Kurihara (Mizuta), Sachi Minagawa, Shiori Nagahara

    　More details

    Applicant：Nagoya University

    Application no：特願2019-076650  Date applied：2019.4

    Announcement no：特開2020-171262  Date announced：2020.10

    Patent/Registration no：特許7334898  Date registered：2023.8 

  8. 植物ゲノム編集方法

    栗原（水多）陽子, 永原史織

    　More details

    Applicant：国立大学法人名古屋大学

    Application no：特願2018-79316  Date applied：2018.4

    Announcement no：2019-180373  Date announced：2019.10

    Patent/Registration no：特許第6614622号  Date registered：2019.11  Date issued：2019.12

    Country of applicant：Domestic  

▼display all

To the head of Industrial property rights.▲
 

Teaching Experience (On-campus) 1

  1. Ph.D. Professional, YM course ヤングメンター制度「花粉の顕微鏡による観察と遺伝子発現」

    2020

    　詳細を見る

    生物の生殖過程では、雄と雌の細胞のダイナミックな動態が見られる。本コースでは、そのうち植物の花粉と呼ばれる雄の生殖細胞に焦点を当てる。様々な植物の花粉を観察し、一過的な遺伝子発現を行うことで、種の多様性と植物の生殖について理解を深める。こうした解析を通じて、細胞培養や顕微鏡観察の技術についても学ぶ。

To the head of Teaching Experience (On-campus).▲
 

Social Contribution 6

  1. 花の中の駆け引きをのぞき見して、命の仕組みを解き明かす！

    Role(s)：Appearance,　Lecturer

    株式会社フロムページ  夢ナビ  2024.8

  2. 植物生殖過程の二光子イメージング

    Role(s)：Lecturer

    コヒレント・ジャパン株式会社; 株式会社ニコンソリューションズ  多光子顕微鏡最新イメージングセミナー  2023.9

  3. Automatic isolation of pollen using NIS-Elements General Analysis (GA) and JOBS imaging workflow tools

    Role(s)：Media coverage,　Informant

    Nikon Solutions Co., Ltd.  Application notes  2023

  4. 「光らせよう！見てみよう！蛍光たんぱく質で科学者体験」

    Role(s)：Lecturer

    サカエ大学Common-S.（運営：松坂屋名古屋店）  松坂屋小学校 第13回キッズサイエンス  名古屋大学 東山キャンパス NIC館  2021.11

    　More details

    Audience： Infants,　Schoolchildren

    Type：Seminar, workshop

    File： 211001_591e5814.jpg

  5. 植物の美：雌しべのなかで色とりどりの花粉管が次世代に命をつなぐ

    Role(s)：Informant

    国立科学博物館、NHK、NHKプロモーション、朝日新聞社  植物展「植物 地球を支える仲間たち」植物の美 展示パネル提供、図録写真提供  2021.7 - 2022.4

  6. 花の神秘 隠されたいのちのしくみ

    Role(s)：Appearance,　Lecturer

    サカエ大学 Common-S.（運営：松坂屋名古屋店）、名古屋大学 学術研究・産学官連携推進本部  名大カフェ "Science, and Me "  2021.4

▼display all

To the head of Social Contribution.▲

Media Coverage 11

  1. A flower’s female sex organs can speed up fertilisation Newspaper, magazine

    The Economist  2024.6

    　More details

    Author：Other 

  2. How plants choose their mates and repel other suitors Internet

    Phys.org  2024.5

    　More details

    Author：Other 

    A group of scientists from Nagoya University in Japan has used a specialized microscopic technique to observe the internal reproduction process of the Arabidopsis plant. Their findings, published in EMBO Reports, reveal the mechanism behind a female flower selectively attracting a single male counterpart. These findings provide insights that may help optimize seed production and improve agricultural breeding practices.

  3. Flowers are picky when choosing their mate for fertilization Internet

    Earth.com  2024.5

    　More details

    Author：Other 

  4. 超進化論 特別版 （１）植物からのメッセージ ～地球を彩る驚異の世界 TV or radio program

    NHK  ＮＨＫスペシャル 超・進化論  開始39分頃  2022.12

    　More details

    最先端の科学が明らかにする“新しい進化の物語”を、圧巻の映像美で描く大型シリーズ。第１集は、陸の王者、植物。「おとなしくて鈍感な生き物」のイメージを覆す、驚異的な能力の数々。植物がまるで“おしゃべり”するかのようにコミュニケーションする様子を、世界初の映像で紹介！さらに、多様な命が暮らす森の地下には“支え合いの世界”が広がっていた！特殊撮影と最先端のＶＦＸ技術で、植物の驚きの新世界を描き尽くす。

  5. 植物透明化技術によるイネの芽や茎の深部の蛍光観察 Internet

    ユサコ株式会社  国内研究者論文紹介  2022.7

  6. めしべの色彩　花火のよう Newspaper, magazine

    読売新聞社  読売新聞  夕刊4版  2021.2

    　More details

    Author：Other 

  7. THE SECRET LIFE OF PLANTS Newspaper, magazine

    National Geographic Society  National Geographic Magazine  page 26  2017.4

    　More details

    Author：Other 

  8. 植物の透明化 Newspaper, magazine

    太田出版  ケトル Vol.30  135ページ  2016.4

  9. 内部構造がまる見え！　植物を透明にする新技術を開発 Newspaper, magazine

    誠文堂新光社  子供の科学  4ページ  2015.12

    　More details

    Author：Other 

  10. Where the Magic Happens Internet

    Cell Press  Cell Picture Show, The Biology of Sex  page 10  2015

    　More details

    Author：Other 

  11. 組み換え技術使わず 名大 花粉管の遺伝子を制御 Newspaper, magazine

    科学新聞社  科学新聞  4面  2014.3

▼display all

To the head of Media Coverage.▲

Academic Activities 5

  1. 「植物細胞の分化運命の制御と可塑性」オーガナイザー

    Role(s)：Planning, management, etc.

    丸山大輔, 水多陽子, 山岡尚平. 日本植物学会第86回大会（共催：学術変革領域研究（B）「細胞運命操作による植物生殖システムのリモデリング」，学術変革領域研究（B）「植物と微生物の共創による超個体の覚醒」）  2022.9

  2. 関連集会「植物生殖改変ワークショップ」オーガナイザー

    Role(s)：Planning, management, etc.

    山岡尚平, 水多陽子, 丸山大輔. 第62回日本植物生理学会年会  2021.3

    　More details

    Type：Competition, symposium, etc. 

  3. 「異分野融合が推進するイメージング研究」オーガナイザー

    Role(s)：Planning, management, etc.

    水多陽子, 多喜正泰. 日本植物学会第84回大会（共催：新学術領域研究「植物新種誕生原理」，認定特定非営利活動法人綜合画像研究支援，日本植物形態学会）  2020.9

    　More details

    Type：Competition, symposium, etc. 

  4. EMBO Workshop "Functional Live Imaging of Plants", Instructor

    2019.5

    　More details

    Type：Academic society, research group, etc. 

  5. 「フィールドにおける植物の理解とその制御に向けた基盤技術創出」オーガナイザー

    Role(s)：Planning, management, etc.

    山口暢俊, 水多陽子, 菅野茂夫. 第60回日本植物生理学会年会（共催：JSTさきがけ「フィールド植物制御」）  2019.3

    　More details

    Type：Competition, symposium, etc. 

To the head of Academic Activities.▲