Updated on 2023/10/12

写真a

 
NATORI Yujin
 
Organization
Graduate School of Medicine Program in Integrated Medicine Social Life Science Assistant Professor
Graduate School
Graduate School of Medicine
Undergraduate School
School of Medicine Department of Medicine
Title
Assistant Professor
Contact information
メールアドレス

Degree 1

  1. 博士(薬学) ( 2017.10   帝京大学 ) 

Research Interests 10

  1. メタボロミクス

  2. シアリダーゼ

  3. メタボローム解析

  4. 脂肪細胞

  5. 分子生物学

  6. Forensic Toxicology

  7. LC/MS/MS

  8. GC/MS/MS

  9. 神経細胞

  10. カンナビノイド

Research Areas 3

  1. Life Science / Pharmaceutical hygiene and biochemistry

  2. Life Science / Pharmaceutical hygiene and biochemistry

  3. Life Science / Forensics medicine  / Forensic Toxicology

Research History 4

  1. 名古屋大学大学院   医学系研究科   助教

    2018.9

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    Country:Japan

  2. Nagoya University   Graduate School of Medicine   Assistant Professor

    2018.9

  3. Teikyo University   Faculty of Pharmaceutical Sciences   Assistant Professor

    2017.11 - 2018.8

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    Country:Japan

  4. Teikyo University   Faculty of Pharma-Science   Assistant

    2011.4 - 2017.10

Committee Memberships 1

  1. 応用物理学会 教育分科会   リフレッシュ理科教室実行委員  

    2012.4 - 2018.9   

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    Committee type:Academic society

 

Papers 10

  1. A simple and rapid method for quantifying aconitines and their metabolites in whole blood by modified QuEChERS and liquid chromatography/tandem mass spectrometry (LC/MS/MS) Reviewed

    Yujin Natori ⁎ , Shoki Kamioka, Takashi Yoshimoto, Akira Ishii

    Forensic Science International     2022.9

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    Authorship:Lead author, Corresponding author   Language:English  

    DOI: https://doi.org/10.1016/j.forsciint.2022.111475

  2. A simple method for the determination of glyphosate, glufosinate and their metabolites in biological specimen by liquid chromatography/tandem mass spectrometry: an application for forensic toxicology. Reviewed

    Tomomi Ohara, Takashi Yoshimoto, Yujin Natori, Akira Ishii

    Nagoya journal of medical science   Vol. 83 ( 3 ) page: 567 - 587   2021.8

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Glyphosate (GLYP) and glufosinate (GLUF) are phosphorus-containing amino acid type herbicides that are used worldwide. With their rising consumptions, fatal intoxication cases due to these herbicides, whether accidental or intentional, cannot be ignored. Both compounds are difficult to detect, and their pretreatment for instrumental analysis are complicated and time-consuming. Our aim was to develop a simple and rapid quantification method for the two herbicides and their metabolites with liquid chromatography/tandem mass spectrometry (LC/MS/MS). We also compared 2-amino-4-phosphonobutyric acid and DL-2-amino-5-phosphonopentanoic acid as alternative internal standards (IS) to GLYP13C2 15N. Herbicide-containing specimens were highly diluted, evaporated to dryness, and derivatized with acetate/acetic anhydride and trimethyl orthoacetate for 30 min. at 120°C. Our optimized LC conditions successfully separated the target analytes, with acceptable linearities (R 2>0.98) and matrix effects (65%-140%). Accuracy and precision ranged from 80.2 % to 111 %, and from 1.3 % to 13 % at the higher concentration, respectively.The concentration of the herbicides and their metabolites were investigated in a postmortem case of suspected herbicide poisoning cases, in which we detected GLYP and its metabolites. Using one of the three ISs, the GLYP concentrations ranged from 3.1 to 3.5 mg/mL, and 3.3 to 4.5 mg/mL in plasma and urine, respectively; GLYP metabolite concentrations in plasma and urine were 18 to 20 μg/mL and 44 to 54 μg/mL. We thus succeeded in developing a rapid method without extraction for measuring GLYP and GLUF along with their metabolites, and demonstrated its practical applicability.

    DOI: 10.18999/nagjms.83.3.567

    Web of Science

    Scopus

    PubMed

  3. NEU1 sialidase controls gene expression and secretion of IL-6 and MCP-1 through NF-κB pathway in 3T3-L1 adipocytes. Reviewed International journal

    Yujin Natori, Miwako Nasui, Kiyoto Edo, Shogo Sato, Takuya Sakurai, Takako Kizaki, Fumiko Kihara-Negishi

    Journal of biochemistry   Vol. 162 ( 2 ) page: 137 - 143   2017.8

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    A sialidase NEU1 that removes sialic acids from glycoconjugates has been implicated in diverse cellular functions. Aberrant NEU1 activity is associated with various pathologies including lysosomal storage disorder sialidosis, autoimmune diseases and the malignancy and metastasis of cancer cells. We recently reported that NEU1 activity increases during 3T3-L1 adipogenesis and that it is higher in the epididymal fat of obese and diabetic mice. However, the precise functions of NEU1 in adipocytes have not been elucidated. Knockdown of NEU1 using siRNA transfection in 3T3-L1 adipocytes significantly decreased the mRNA expression and protein secretion of IL-6 and MCP-1 induced by LPS. The promoter activities of both IL-6 and MCP-1 as well as nuclear factor-kappa B (NF-κB) nuclear translocation were reduced in adipocytes transfected with an siRNA sequence that targets NEU1(siNEU1). NEU1 suppression using siNEU1 affected TLR4 sialylation. These findings suggest that NEU1 is involved in the production of IL-6 and MCP-1 in adipocytes possibly through TLR4/NF-κB signalling.

    DOI: 10.1093/jb/mvx006

    PubMed

  4. Neu1 sialidase interacts with perilipin 1 on lipid droplets and inhibits lipolysis in 3T3-L1 adipocytes. Reviewed International journal

    Yujin Natori, Miwako Nasui, Fumiko Kihara-Negishi

    Genes to cells : devoted to molecular & cellular mechanisms   Vol. 22 ( 5 ) page: 485 - 492   2017.5

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Fatty acids are stored within adipocytes in lipid droplets (LDs) as triacylglycerol (TG), which is converted to free fatty acid (FFA) and glycerol via lipolysis. Increased plasma FFA levels in obesity are associated with several clinical conditions. We previously found that Neu1 activity is aberrant in the epididymal fat and liver of obese and diabetic mice. Here, we examined involvement of Neu1 in lipolysis in 3T3-L1 adipocytes. Small interfering RNA against Neu1 was introduced into adipocytes, and glycerol concentrations were measured in the culture medium. We then assessed the effects of Neu1 knockdown on lipolytic protein expression and phosphorylation, as well as interactions between perilipin 1 (Plin1) and hormone-sensitive lipase (HSL) after isoproterenol (IS) stimulation. Interactions between Neu1 and Plin1 were analyzed by immunoprecipitation and immunofluorescent imaging using adipocytes transfected with pCMV6-mNeu1-myc-DYKDDDDK (mNeu1DDK). Neu1 knockdown increased glycerol concentrations in culture media and Plin1 phosphorylation in whole lysates of IS-stimulated cells. Neu1 knockdown increased interaction between Plin1 and HSL after IS stimulation whereas that between Neu1 and Plin1 on LD observed under basal conditions was lost. These results suggest that Neu1 inhibits lipolysis induced by β-adrenergic stimulation in adipocytes via interactions with Plin1 on LD.

    DOI: 10.1111/gtc.12490

    PubMed

  5. Acidic sialidase activity is aberrant in obese and diabetic mice. Reviewed

    Yujin Natori, Naoki Ohkura, Miwako Nasui, Gen-ichi Atsumi, Fumiko Kihara-Negishi

    Biological & pharmaceutical bulletin   Vol. 36 ( 6 ) page: 1027 - 31   2013

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    Mammalian sialidases (NEU1, NEU2, NEU3 and NEU4) that remove sialic acids from glycoconjugates have been implicated in diverse cellular functions. Human sialidases are involved in the development of various disease states such as cancer, diabetes and arteriosclerosis. Unregulated acidic sialidase NEU1 activity is associated with the pathogenesis of lysosomal storage disorder (LSD) sialidosis, abnormal immune responses and cancer progression. Obesity is closely related to several chronic diseases such as diabetes, cardiovascular diseases, hyperlipidemia or hypertension that are associated with metabolic syndrome. We examined fluctuations in mRNA levels and sialidase activities of NEU1 in two strains of obese and diabetic mice to assess the involvement of NEU1 in obesity. The activity of NEU1 was preferentially higher in epididymal fat and lower in the livers of two strains of obese and diabetic mice. Fluctuations in NEU1 activity might be associated with the pathological status of these tissues in obesity.

    DOI: 10.1248/bpb.b12-00995

    PubMed

  6. Identification and quantification of diphenhydramine, haloperidol, and its metabolite, reduced haloperidol in a saponified brain specimen that was immersed in the sea water for more than 10 years Reviewed

    Yujin Natori, Takashi Yoshimoto, Toshimichi Yamamoto, Akira Ishii

    Legal Medicine   Vol. 61   2023.3

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  7. Comparisons between Japanese and Han Chinese populations for 261 autosomal STR loci Reviewed

    T. Yamamoto, K. Doi, R. Fukami, T. Yoshimoto, Y. Natori, A. Ishii

    Forensic Science International: Genetics Supplement Series   Vol. 8 ( 1 ) page: 117 - 119   2022.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.fsigss.2022.10.003

    Web of Science

    Scopus

  8. Triiodothyronine Aggravates Global Cerebral Ishemia-Reperfusion Injury in Mice Reviewed

    Masaru Doshi, Shiro Watanabe, Yujin Natori, Makoto Hosoyamada, and Yutaka Hirashima-Akae

    Biological and Pharmaceutical Bulletin   Vol. 44 ( 12 ) page: 1823 - 1831   2021.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1248/bpb.b21-00424

  9. 労働現場で発生したトルエン中毒による死亡の二事例

    石井 晃、名取 雄人

    産業医学ジャーナル   Vol. 43 ( 5 ) page: 32 - 35   2020.9

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

  10. Development of "Quick-DB forensic": A total workflow from QuEChERS-dSPE method to GC-MS/MS quantification of forensically relevant drugs and pesticides in whole blood. Reviewed International journal

    Maiko Kusano, Yuki Sakamoto, Yujin Natori, Haruhiko Miyagawa, Hitoshi Tsuchihashi, Akira Ishii, Kei Zaitsu

    Forensic science international   Vol. 300   page: 125 - 135   2019.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    Trends in forensic toxicology show the advancement of rapid and sensitive analytical methods for qualitative and quantitative analysis of drugs of abuse. However, forensic toxicologists are continuously faced with the challenges of identifying and quantifying drug blood concentration while simultaneously struggling with manpower shortage. In view of developing a simple and productive toxicological analysis method encompassing total workflow from sample preparation to quantitative analysis, here we describe a simple, robust, and sensitive method for the simultaneous determination and quantification of 63 forensically relevant drugs and pesticides in human whole blood. The method is based on sample preparation by a modified QuEChERS extraction and dispersive solid-phase extraction (dSPE) clean-up followed by gas chromatography-tandem mass spectrometry (GC-MS/MS) analysis. Limits of detection of the target analytes in whole blood ranged in the few ng/mL-order levels. Intra- and inter-day validation result ranges were 0-24% for accuracy (% error) and 0.8-26% for precision (%RSD). Recovery rates ranged from 66% to 84% for barbiturates, 36% to 110% for benzodiazepines, 41% to 86% for tri/tetracyclic antidepressants, 15% to 81% for drugs of abuse, 28% to 44% for phenethylamines, and 25% to 118% for pesticides. The validated results were used to develop a user-friendly, systematic, and quantitative toxicological GC/MS/MS system and software "Quick-DB Forensic".

    DOI: 10.1016/j.forsciint.2019.03.048

    Web of Science

    Scopus

    PubMed

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Books 2

  1. NeuroPsychopharmacotherapy

    Akira Ishii, Yujin Natori( Role: Joint author ,  International Regulations and Regulating Laws in Japan)

    Springer  2022.11  ( ISBN:9783030620585

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    Total pages:4784   Language:Japanese

    ASIN

  2. Neuropsychopharmacotherapy: International Regulations and Regulating Laws in Japan

    Ishii A., Natori Y.( Role: Sole author)

    NeuroPsychopharmacotherapy  2022.1  ( ISBN:9783030620585

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    Language:Japanese

    Psychopharmaceuticals contain various drugs of which chemical structures and pharmacological effects are different. Each substance varies in usefulness for medication purposes as well as risk of abuse. Various substance abuse countermeasures have thus been implemented internationally and within each country. In this chapter, the three main International Conventions for controlling and regulating narcotics and psychotropics are outlined: the Single Convention on Narcotic Drugs of 1961, the Convention on Psychotropic Drugs of 1971, and the Convention against Illicit Traffic in Narcotic Drugs and Psychotropic Substances of 1988. The history on the abuse of narcotics and psychotropics in Japan is explained, and their countermeasures including the four main domestic laws are then presented: the Stimulants Control Act, the Narcotics and Psychotropic Drugs Control Act, the Cannabis Control Act, and the Opium Control Act. Finally, specific topics including the issues on benzodiazepines including etizolam and on synthetic cannabinoids in Japan are described.

    DOI: 10.1007/978-3-030-62059-2_386

    Scopus

MISC 29

  1. A simple and rapid method for quantifying aconitines and their metabolites in whole blood by modified QuEChERS and liquid chromatography/tandem mass spectrometry (LC/MS/MS). International journal

    Yujin Natori, Shoki Kamioka, Takashi Yoshimoto, Akira Ishii

    Forensic science international   Vol. 341   page: 111475 - 111475   2022.12

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    Authorship:Lead author, Corresponding author   Language:English  

    Aconitum contains highly toxic alkaloids such as aconitine, hypaconitine, jesaconitine, and mesaconitine. Since Aconitum ingestion causes fatal intoxication, it is important to analyze aconitines and their metabolites in the blood. In forensic toxicology, postmortem drug redistribution is known as one factor that would hamper accurate evaluation of concentrations. Therefore, it is recommended to collect multiple blood samples from various sites and compare the results to avoid miss identification of causative compounds for intoxication. In this study, we evaluated aconitines and their metabolites in postmortem blood specimens from ten sites by QuEChERS extraction and liquid chromatography-tandem mass spectrometry (LC/MS/MS). The recovery rates and matrix effects of analytes were approximately 74-80% and 94-100%, respectively. The correlation coefficients were over 0.99. The validation studies revealed that accuracies and precisions were around 97-2% (intraday) and 100-4% (interday), respectively. Finally, the concentrations of aconitine and jesaconitine were from 2.72 to 7.20 ng/mL and from 14.9 to 26.3 ng/mL, respectively. The concentrations of mesaconitine were from 0.32 to 0.88 ng/mL in four samples and detected in two. The concentrations were highest in the right atrium and lowest in the femoral vein. Our results suggest that aconitine and jesaconitne are accumulated in right atrium blood after death, and that right atrium specimen is suitable for measuring aconitine compounds in fatal intoxication cases.

    DOI: 10.1016/j.forsciint.2022.111475

    Web of Science

    Scopus

    PubMed

  2. The effect of endocannabinoid and synthetic cannabinoid in metabolome profiles for the neuroblastoma cell line

    Yujin NATORI, Akira ISHII

        2023.3

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    Authorship:Lead author  

  3. 脳虚血マウスの海馬および血清のメタボローム解析

    道志 勝, 名取 雄人, 石井 晃, 渡辺 志朗, 細山田 真, 赤江 豊

    日本薬学会要旨集 第143年会     2023.3

  4. トリカブト根喫食による死亡例における全血中アコニチン類とその代謝物の死後再分布の分析

    名取雄人, 上岡翔輝, 吉本高士, 石井晃

    日本法医学会学術全国集会講演要旨集   Vol. 106th   2022.6

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    J-GLOBAL

  5. 約20年前の性的暴行事件の再審請求に関する鑑定

    山本敏充, 吉本高士, 名取雄人, 石井晃

    日本法医学会学術全国集会講演要旨集   Vol. 106th   2022.6

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  6. 液体クロマトグラフィータンデム質量分析計(LC/MS/MS)を用いた血中アコニチン類の簡便な分析方法の確立と本方法を用いた死後再分布の検証

    名取 雄人, 上岡 翔輝, 吉本 高士, 石井 晃

    日本薬学会年会要旨集   Vol. 142年会   page: 28PO10 - 18   2022.3

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    Authorship:Lead author   Language:Japanese   Publisher:(公社)日本薬学会  

  7. 甲状腺ホルモン投与がマウス脳虚血再灌流後の海馬TGF-β1遺伝子発現に及ぼす影響

    道志 勝, 渡辺 志朗, 名取 雄人, 細山田 真, 赤江 豊

    日本薬学会年会要旨集   Vol. 142年会   page: 28PO8 - 12   2022.3

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  8. マウス脳虚血再灌流後の神経細胞死の発生に対するトリヨードチロニンの悪化作用

    道志 勝, 渡辺 志朗, 名取 雄人, 細山田 真, 赤江 豊

    日本薬学会年会要旨集   Vol. 141年会   page: 28P02 - 145   2021.3

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    Language:Japanese   Publisher:(公社)日本薬学会  

  9. Y-STRsハプロタイプから日本人に由来する男性であることを推定した例

    山本敏充, 吉本高士, 名取雄人, 安達登, 石井晃

    日本法医学会学術全国集会講演要旨集   Vol. 105th   2021

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    Language:Japanese   Publishing type:Research paper, summary (national, other academic conference)  

    J-GLOBAL

  10. LC/MS/MSを用いたグリホサート,グルホシネートとその代謝物の簡便な定量方法の構築

    金春子, 大原倫美, 吉本高士, 名取雄人, 石井晃

    日本法医学会学術全国集会講演要旨集   Vol. 105th   2021

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  11. ロボットアームによる頸部圧迫の一例

    石井 晃, 吉本 高士, 山本 敏充, 財津 桂, 名取 雄人

    日本法医学雑誌   Vol. 74 ( 1 ) page: 88 - 88   2020.8

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    Language:Japanese   Publisher:(NPO)日本法医学会  

  12. 甲状腺ホルモンがマウス脳虚血再灌流後の神経細胞死の発生に及ぼす影響

    道志 勝, 渡辺 志朗, 名取 雄人, 細山田 真, 赤江 豊

    日本薬学会年会要旨集   Vol. 140年会   page: 27P - pm176   2020.3

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  13. Comparison of internal standards for determining glyphosate, glufosinate and their metabolites in human Plasma by LC-MS/MS Reviewed

    Ishii A, Zaitsu K, Natori Y, Tsuchihashi H

    The 57th Annual Meeting of The International Association of Forensic Toxicologists (TIAFT)     2019.9

  14. Non-target screening method for drugs in blood by combinational use of LC/Q-TOFMS and Micro Volume QuEChERS kit Reviewed

    Yujin Natori, Eishi Imoto, Kengo Matsumoto, Mikaël Levi, Hitoshi Tsuchihashi, Akira Ishii, Tairo Ogura, Kei Zaitsu

    The 57th Annual Meeting of The International Association of Forensic Toxicologists (TIAFT)     2019.9

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    Authorship:Lead author   Language:English   Publishing type:Research paper, summary (international conference)  

  15. LC/Q-TOFMS と Micro Volume QuEChERS を用いた全血中薬物ノンターゲットスクリーニ ング分析法の構築

    名取 雄人, 井本 英志, Levi Mikaël, 土橋 均, 石井 晃, 小倉 泰郎, 財津 桂

    日本法中毒学会第38年会要旨集     2019.7

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    Authorship:Lead author   Language:Japanese   Publishing type:Research paper, summary (national, other academic conference)  

  16. Microflow-LC/MS/MS および Micro volume QuEChERS による極少量血液を用いた薬物の分析

    松本 謙吾, Levi Mikaël, 井本 英志, 名取 雄人, 土橋 均, 石井 晃, 小倉 泰郎財津

    日本法中毒学会第38年会要旨集     2019.7

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  17. Evaluation of Micro Volume Sample Preparation Technology Newly Designed for Forensic Toxicology with High Resolution Accurate Mass Spectrometry

    Eishi Imoto, Yujin Natori, Jun Watanabe, Hitoshi Tsuchihashi, Kei Zaitsu, Ichiro Hirano

    67th ASMS Conference on Mass Spectrometry     2019.6

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    Language:English   Publishing type:Research paper, summary (international conference)  

  18. 労働現場で発生したトルエン中毒の二死亡例

    石井 晃, 吉本 高士, 名取 雄人, 山本 敏充, 財津 桂

    日本法医学雑誌   Vol. 73 ( 1 ) page: 104 - 104   2019.5

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  19. 自動前処理装置ATLASと高感度LC-MS/MSを用いた「全自動血中農薬分析システム」の構築

    丸地 正人, 松本 謙吾, 石丸 麗子, 地中 啓, 三木 昭宏, 片木 宗弘, 名取 雄人, 土橋 均, 財津 桂, 石井 晃

    日本法医学雑誌   Vol. 73 ( 1 ) page: 90 - 90   2019.5

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  20. 気づきの体験学習がもたらす協同作業認識とディスカッションスキルの認識の変化

    大竹隼生, 長谷川仁美, 高塚人志, 奥秋美香, 名取雄人, 岩澤晴代, 楯直子, 岸本成史

    第3回日本薬学教育学会大会要旨集     2018.9

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  21. 主体的な理科教室運営による学生への教育効果

    名取 雄人, 光井 俊治, 那須井 美和子

    日本薬学会年会要旨集   Vol. 138年会 ( 4 ) page: 232 - 232   2018.3

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  22. NEU1はペリリピン1とホルモン感受性リパーゼ(HSL)の相互作用を制御し脂肪分解を抑制する

    名取 雄人, 那須井 美和子, 根岸 文子

    生命科学系学会合同年次大会   Vol. 2017年度   page: [2P - 0002]   2017.12

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    Authorship:Lead author   Language:Japanese   Publisher:生命科学系学会合同年次大会運営事務局  

  23. シアリダーゼNeu1による脂肪分解制御機構の解明

    名取 雄人, 那須井 美和子, 根岸 文子

    日本薬学会年会要旨集   Vol. 137年会 ( 3 ) page: 130 - 130   2017.3

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  24. 糖分解酵素Neu1による炎症性サイトカインの分泌制御

    名取 雄人, 佐藤 章悟, 櫻井 拓也, 木崎 節子, 鈴木 光浩, 那須井 美和子[齋藤], 根岸 文子[木原]

    日本生化学会大会・日本分子生物学会年会合同大会講演要旨集   Vol. 88回・38回   page: [2P1239] - [2P1239]   2015.12

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    Authorship:Lead author   Language:Japanese   Publisher:(公社)日本生化学会  

  25. シアリダーゼNeu1の炎症性サイトカイン分泌に関わる機能の解析

    名取 雄人, 那須井 美和子, 根岸 文子

    日本薬学会年会要旨集   Vol. 135年会 ( 3 ) page: 172 - 172   2015.3

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  26. シアリダーゼNeul変動が脂肪細胞の機能と肥満に及ぼす影響

    名取 雄人, 那須井 美和子, 根岸 文子

    日本薬学会年会要旨集   Vol. 134年会 ( 4 ) page: 138 - 138   2014.3

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  27. シアリダーゼNeu1によるアディポカイン分泌と脂肪分解の制御

    名取 雄人, 那須井 美和子, 曽賀 千智, 宮城 妙子, 江戸 清人, 根岸 文子

    日本薬学会年会要旨集   Vol. 133年会 ( 3 ) page: 91 - 91   2013.3

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  28. シアリダーゼNeu1による脂肪細胞の機能調節と肥満への関与の解析

    名取雄人, 那須井美和子, 根岸文子

    日本薬学会関東支部大会講演要旨集   Vol. 57th   2013

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    J-GLOBAL

  29. 食虫植物の胃袋を手作り内視鏡で観察する!~“内視鏡による胃の観察モデル”作成の試み~

    和田洸司, 光井俊治, 馬塲養治, 杉山智恵, 渡部有佳, 石塚裕之, 高橋和子, 名取雄人, 根岸文子, 那須井美和子

    日本薬学会関東支部大会講演要旨集   Vol. 57th   2013

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KAKENHI (Grants-in-Aid for Scientific Research) 6

  1. Development of a multdimentional palatform for assessing effects and toxicity of active compounds in cannabis

    Grant number:23K18387  2023.6 - 2025.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

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    Authorship:Coinvestigator(s) 

  2. Elusidation for Toxicity Mechanism of Synthetic Cannabinoids by Multi-omics Approach: Focusing on Cardiovascular System

    Grant number:23K09762  2023.4 - 2026.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Principal investigator 

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

  3. Elucidation of acute poisoning mechanism due to abuse of CB1 receptor agonist.

    Grant number:21K17323  2021.4 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists  Grant-in-Aid for Early-Career Scientists

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    Authorship:Principal investigator 

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

  4. 分解後プロテオミクス:法医学からの新規プロテオミクス

    Grant number:19K22756  2019.6 - 2022.3

    日本学術振興会  科学研究費助成事業 挑戦的研究(萌芽)  挑戦的研究(萌芽)

    石井 晃, 名取 雄人, 名取 雄人

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    Authorship:Coinvestigator(s) 

    本研究では、質量分析法を用いたペプチド解析を行うことにより、最初はトリプシン消化フラグメントを用いて、従来の法医生化学検査では測定不能であった試料における目的タンパクを定量し、次いで、実検体で切断されているペプチドフラグメントを同定し、法医診断に資するタンパク(ペプチド)マーカーの探索を行うことを目的とする。対象となるタンパクとしては、熱中症で上昇するミオグロビン、心不全で上昇するNT-proBNPが挙げられる。
    最初の目標は尿中ミオグロビンであり、現在、標準品ヒトミオグロビンのトリプシン消化フラグメントの解析を行うことにより、実験条件の検討を行っている。トリプシン消化により、ヒトミオグロビンは19のフラグメントに分解し、それぞれについて、MS/MS上でのトランジションの最適化を行った。次いで、これらフラグメントをセミミクロカラム及びミクロカラムで分離し、分離性能及び感度を比較した。セミミクロカラムによる分離と比較すると、ミクロカラムによる分離を行うことにより、分離時間は30分から10分と短縮でき、面積値で1.5倍から9倍程度の感度の上昇が認められ、ミクロカラムがこれらフラグメントの検出に有用であることが示された。現在、標準尿に標準ミオグロビンを添加したものを室温で放置し、その後トリプシン消化を行い、ペプチドの絶対強度を比較した。すると、ペプチド間で強度に差が見られ、定量に適したペプチドが選択できる可能性が示唆された。また、同時にSDS-PAGEによるミオグロビンの分離を行い、分解速度検討のための基礎データを採取している。

  5. Elucidation of acute poisoning mechanism due to abuse of CB1 receptor agonist.

    Grant number:19K19485  2019.4 - 2021.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Early-Career Scientists

    NATORI YUJIN

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    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    In this study, we investigated the effects of CB1 receptor agonists, which are abused, on CB1 receptor expression and metabolism using cultured cells. For the aim of this study, we used Neuro2A, a mouse-derived neuroblastoma, to investigate the effects of changes in the culture environment on CB1 receptor expression and the effects of CB1 receptor agonists on the metabolome.As a result, the mRNA expression of the CB1 receptor fluctuated due to the decrease in serum in the medium. In addition, HU-210 and AM2210 significantly changed 40 components including sugars, lipids, amino acids and their metabolites. It is considered that such fluctuations in metabolism may cause dysfunction of cells and tissues including cell death.

  6. Accurate measurement of natural toxins in body fluids using deuterated standards and search of specific markers for the rapid diagnosis

    Grant number:18H03064  2018.4 - 2021.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Ishii Akira

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    Authorship:Coinvestigator(s) 

    The purpose of the present study is to develop methods for sensitive identification and precise quantification of natural toxins in human biological specimens using deuterium labeled natural toxins by deuterium exchange reactions. Although we have not obtained deuterium labeled natural toxins, we have developed two methods for screening, identifying and quantification natural toxins in human biological specimens: a screening system for 56 natural toxins by liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-QTOFMS), and a sensitive quantification method for 12 plant toxins by LC-tandem mass spectrometry, combined with simple solid-phase extraction.

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Teaching Experience (On-campus) 4

  1. ベーシックトレーニング(薬毒物分析)

    2020

  2. 社会医学実習

    2020

  3. 社会医学実習

    2019

  4. 医学入門

    2019