Updated on 2022/10/31

写真a

 
MATSUYAMA Shuichi
 
Organization
Graduate School of Bioagricultural Sciences Field Science Center Division Associate professor
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Bioresource Sciences
Title
Associate professor

Degree 1

  1. 博士(農学) ( 2005.6   名古屋大学 ) 

Research Interests 3

  1. 繁殖

  2. 神経内分泌

  3. 子宮

Research Areas 3

  1. Life Science / Animal production science

  2. Life Science / Animal production science

  3. Life Science / Animal production science

Current Research Project and SDGs 1

  1. 牛の低受胎要因解明と生産性向上技術の開発

Professional Memberships 3

  1. 日本繁殖生物学会

  2. 日本畜産学会

  3. Society for the Study of Reproduction

Committee Memberships 11

  1. 公益社団法人 日本繁殖生物学会   評議員  

    2021.9 - 2023.8   

  2. 公益社団法人 日本繁殖生物学会   将来計画検討委員  

    2021.9 - 2023.8   

  3. 公益社団法人 日本繁殖生物学会   広報委員  

    2021.9 - 2023.8   

  4. 公益社団法人 日本繁殖生物学会   庶務幹事  

    2021.9 - 2023.8   

  5. 東海畜産学会   評議員  

    2021.4 - 2023.3   

  6. 公益社団法人 日本繁殖生物学会   評議員  

    2019.9 - 2021.8   

  7. 公益社団法人 日本繁殖生物学会   将来計画検討委員  

    2019.9 - 2021.8   

  8. 公益社団法人 日本繁殖生物学会   庶務幹事  

    2019.9 - 2021.8   

  9. 公益社団法人 日本繁殖生物学会   広報委員  

    2019.9 - 2021.8   

  10. 畜産学教育協議会   幹事  

    2019.4 - 2021.3   

  11. 東海畜産学会   評議員  

    2019.4 - 2021.3   

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Awards 2

  1. NARO RESEARCH PRIZE

    2016.9  

  2. 第94回日本繁殖生物学会大会 優秀ポスター賞

    2001.9   日本繁殖生物学会  

    松山秀一

 

Papers 53

  1. A Survey of Genome-Wide Genetic Characterizations of Crossbred Dairy Cattle in Local Farms in Cambodia

    Mam Somony, Tep Bengthay, Rin Soriya, Uenoyama Yoshihisa, Matsuyama Shuichi, Ohkura Satoshi, Murase Tetsuma, Nunome Mitsuo, Morita Yasuhiro

    ANIMALS   Vol. 12 ( 16 )   2022.8

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    Language:Japanese   Publisher:Animals  

    To improve the dairy sector in Cambodia in the future, we aimed to reveal the genetic variation and the milk production in Cambodian crossbred dairy cattle. We calculated the percent (%) milk fat content and the average milk yield per cow (L/day) for two farms (Farm R and M) based on the farmers’ records and interviews. The crossbred cows originated from Cambodian local farmers and Thailand breeders in Farm R, whereas the crossbred cows originated in Thailand breeders in Farm M. Then, we performed genetic characterization for 75 individuals from the two farms and an individual Japanese pure Holstein-Friesian cow based on 133,705 single nucleotide polymorphisms (SNPs) obtained by the GRAS-Di method. The milk fat contents in the bulk milk in the dry season and the average milk yield per cow on Farm R were 3.77 ± 0.98% and 7.81 ± 2.66 L/day, respectively, and were higher than those on Farm M (3.35 ± 0.54% and 6.5–7.5 L/day). Cattle originating in Cambodia in Farm R possessed a unique genetic character different from cattle from Thailand in Farm M. The present study suggests that the differences in milk fat content between the two farms might be explained by the genetic differences in crossbred cows.

    DOI: 10.3390/ani12162072

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  2. Gene-expression profile and postpartum transition of bovine endometrial side population cells†

    Ryoki Tatebayashi, Sho Nakamura, Shiori Minabe, Tadashi Furusawa, Ryoya Abe, Miki Kajisa, Yasuhiro Morita, Satoshi Ohkura, Koji Kimura, Shuichi Matsuyama

    Biology of Reproduction   Vol. 104 ( 4 ) page: 850 - 860   2021.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    <title>Abstract</title>
    The mechanism of bovine endometrial regeneration after parturition remains unclear. Here, we hypothesized that bovine endometrial stem/progenitor cells participate in the postpartum regeneration of the endometrium. Flow cytometry analysis identified the presence of side population (SP) cells among endometrial stromal cells. Endometrial SP cells were shown to differentiate into osteoblasts and adipocytes. RNA-seq data showed that the gene expression pattern was different between bovine endometrial SP cells and main population cells. Gene Set Enrichment Analysis identified the enrichment of stemness genes in SP cells. Significantly (false discovery rate &amp;lt; 0.01) upregulated genes in SP cells contained several stem cell marker genes. Gene Ontology (GO) analysis of the upregulated genes in SP cells showed enrichment of terms related to RNA metabolic process and transcription. Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis of upregulated genes in SP cells revealed enrichment of signaling pathways associated with maintenance and differentiation of stem/progenitor cells. The terms involved in TCA cycles were enriched in GO and KEGG pathway analysis of downregulated genes in SP cells. These results support the assumption that bovine endometrial SP cells exhibit characteristics of somatic stem/progenitor cells. The ratio of SP cells to endometrial cells was lowest on days 9–11 after parturition, which gradually increased thereafter. SP cells were shown to differentiate into epithelial cells. Collectively, these results suggest that bovine endometrial SP cells were temporarily reduced immediately after calving possibly due to their differentiation to provide new endometrial cells.

    DOI: 10.1093/biolre/ioab004

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  3. Neurokinin 3 receptor–selective agonist, senktide, decreases core temperature in Japanese Black cattle Reviewed

    S. Nakamura, M. Miwa, Y. Morita, S. Ohkura, T. Yamamura, Y. Wakabayashi, S. Matsuyama

    Domestic Animal Endocrinology   Vol. 74   page: 106522 - 106522   2021.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.domaniend.2020.106522

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  4. Seasonal changes in the reproductive performance in local cows receiving artificial insemination in the Pursat province of Cambodia. Reviewed International journal

    Bengthay Tep, Yasuhiro Morita, Shuichi Matsuyama, Satoshi Ohkura, Naoko Inoue, Hiroko Tsukamura, Yoshihisa Uenoyama, Vutha Pheng

    Asian-Australasian journal of animal sciences   Vol. 33 ( 12 ) page: 1922 - 1929   2020.12

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    Objective: The present study aimed to survey seasonal changes in reproductive performance in local cows receiving artificial insemination (AI) in the Pursat province of Cambodia, a tropical country, to investigate if ambient conditions affect the reproductive performance of cows as to better understand the major problems regarding cattle production. Methods: The number of cows receiving AI, resultant number of calving and calving rate were analyzed for those receiving the first AI from 2016 to 2017. The year was divided into three seasons: cool/dry (from November to February), hot/dry (from March to June), and wet (from July to October), based on the maximal temperature and rainfall in Pursat, to analyze the relationship between ambient conditions and the reproductive performance of cows. Body condition scores (BCS) and feeding schemes were also analyzed in these seasons. Results: The number of cows receiving AI was significantly higher in the cool/dry season than the wet season. The number of calving and calving rate were significantly higher in cows receiving AI in the cool/dry season compared with the hot/dry and wet seasons. The cows showed higher BCSs in the cool/dry season compared to the hot/dry and wet seasons probably due to the seasonal changes in the feeding schemes: These cows grazed on wild grasses in the cool/dry season but fed with a limited amount of grasses and straw in the hot/dry and wet seasons. Conclusion: The present study suggests that the low number of cows receiving AI, low number of calving and low calving rate could be mainly due to poor body condition as a result of the poor feeding schemes during the hot/dry and wet seasons. The improvement of body condition by the refinement of feeding schemes may contribute to an increase in the reproductive performance in cows during the hot/dry and wet seasons in Cambodia.

    DOI: 10.5713/ajas.19.0893

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  5. Facilitatory and inhibitory role of central amylin administration in the regulation of the gonadotropin-releasing hormone pulse generator activity in goats. Reviewed International journal

    Yuri Kitagawa, Takuya Sasaki, Reika Suzumura, Ai Morishima, Ryoki Tatebayashi, Assadullah, Nahoko Ieda, Yasuhiro Morita, Shuichi Matsuyama, Naoko Inoue, Yoshihisa Uenoyama, Hiroko Tsukamura, Satoshi Ohkura

    Neuroscience letters   Vol. 736   page: 135276 - 135276   2020.9

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    Pulsatile gonadotropin-releasing hormone (GnRH) secretion is essential for regulating reproductive functions in mammals. GnRH pulses are governed by a neural mechanism that is termed the GnRH pulse generator. In the present study, we investigated the role of central calcitonin receptor (CTR) signaling in the regulation of the GnRH pulse generator activity in ovariectomized goats by administering amylin, an endogenous ligand for CTR, into the lateral ventricle. GnRH pulse generator activity was measured using multiple unit activity (MUA) recordings in the mediobasal hypothalamus. We analyzed changes in the interval of characteristic increases in MUA (MUA volleys). The MUA volley interval shortened immediately after amylin administration, followed by prolonged intervals. Double in situ hybridization for KISS1 (kisspeptin gene) and CALCR (CTR gene) revealed that low expression levels of CALCR were found in the arcuate kisspeptin neurons, which is suggested as the main population of neurons, involved in GnRH pulse generator activity. These results suggest that central amylin-CTR signaling has a biphasic role in the regulation of GnRH pulse generator activity by acting on cells other than the arcuate kisspeptin neurons in goats.

    DOI: 10.1016/j.neulet.2020.135276

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  6. Peripheral administration of SB223412, a selective neurokinin-3 receptor antagonist, suppresses pulsatile luteinizing hormone secretion by acting on the gonadotropin-releasing hormone pulse generator in estrogen-treated ovariectomized female goats.

    Takuya Sasaki, Tomoya Sonoda, Ryoki Tatebayashi, Yuri Kitagawa, Shinya Oishi, Koki Yamamoto, Nobutaka Fujii, Naoko Inoue, Yoshihisa Uenoyama, Hiroko Tsukamura, Kei-Ichiro Maeda, Fuko Matsuda, Yasuhiro Morita, Shuichi Matsuyama, Satoshi Ohkura

    The Journal of reproduction and development   Vol. 66 ( 4 ) page: 351 - 357   2020.8

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    Accumulating evidence suggests that KNDy neurons located in the hypothalamic arcuate nucleus (ARC), which are reported to express kisspeptin, neurokinin B, and dynorphin A, are indispensable for the gonadotropin-releasing hormone (GnRH) pulse generation that results in rhythmic GnRH secretion. The aims of the present study were to investigate the effects of peripheral administration of the neurokinin 3 receptor (NK3R/TACR3, a receptor for neurokinin B) antagonist, SB223412, on GnRH pulse-generating activity and pulsatile luteinizing hormone (LH) secretion in ovariectomized Shiba goats treated with luteal phase levels of estrogen. The NK3R antagonist was infused intravenously for 4 h {0.16 or 1.6 mg/(kg body weight [BW]·4 h)} during which multiple unit activity (MUA) in the ARC was recorded, an electrophysiological technique commonly employed to monitor GnRH pulse generator activity. In a separate experiment, the NK3R antagonist (40 or 200 mg/[kg BW·day]) was administered orally for 7 days to determine whether the NK3R antagonist could modulate pulsatile LH secretion when administered via the oral route. Intravenous infusion of the NK3R antagonist significantly increased the interval of episodic bursts of MUA compared with that of the controls. Oral administration of the antagonist for 7 days also significantly prolonged the interpulse interval of LH pulses. The results of this study demonstrate that peripheral administration of an NK3R antagonist suppresses pulsatile LH secretion by acting on the GnRH pulse generator, suggesting that NK3R antagonist administration could be used to modulate reproductive functions in ruminants.

    DOI: 10.1262/jrd.2019-145

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  7. Peripheral administration of SB223412, a selective neurokinin-3 receptor antagonist, suppresses pulsatile luteinizing hormone secretion by acting on the gonadotropin-releasing hormone pulse generator in estrogen-treated ovariectomized female goats Reviewed

    Sasaki Takuya, Sonoda Tomoya, Tatebayashi Ryoki, Kitagawa Yuri, Oishi Shinya, Yamamoto Koki, Fujii Nobutaka, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko, Maeda Kei-ichiro, Matsuda Fuko, Morita Yasuhiro, Matsuyama Shuichi, Ohkura Satoshi

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   Vol. 66 ( 4 ) page: 351 - 357   2020.8

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  8. Establishment of immortalised cell lines derived from female Shiba goat KNDy and GnRH neurones Reviewed

    Yuta Suetomi, Ryoki Tatebayashi, Shuhei Sonoda, Arisa Munetomo, Shuichi Matsuyama, Naoko Inoue, Yoshihisa Uenoyama, Yukari Takeuchi, Hiroko Tsukamura, Satoshi Ohkura, Fuko Matsuda

    JOURNAL OF NEUROENDOCRINOLOGY   Vol. 32 ( 6 ) page: e12857   2020.6

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    Kisspeptin plays a critical role in governing gonadotrophin-releasing hormone (GnRH)/gonadotrophin secretion and subsequent reproductive function in mammals. The hypothalamic arcuate nucleus (ARC) kisspeptin neurones, which co-express neurokinin B (NKB) and dynorphin A (Dyn) and are referred to as KNDy neurones, are considered to be involved in GnRH generation. The present study aimed to establish cell lines derived from goat KNDy and GnRH neurones. Primary-cultured cells of female Shiba goat foetal hypothalamic ARC and preoptic area (POA) tissues were immortalised with the infection of lentivirus containing the simian virus 40 large T-antigen gene. Clones of the immortalised cells were selected by the gene expression of a neuronal marker, and then the neurone-derived cell clones were further selected by the gene expression of KNDy or GnRH neurone markers. As a result, we obtained a KNDy neurone cell line (GA28) from the ARC, as well as two GnRH neurone cell lines (GP11 and GP31) from the POA. Immunocytochemistry revealed the expression of kisspeptin, NKB and Dyn in GA28 cells, as well as GnRH in GP11 and GP31 cells. GnRH secretion from GP11 and GP31 cells into the media was confirmed by an enzyme immunoassay. Moreover, kisspeptin challenge increased intracellular Ca2+ levels in subsets of both GP11 and GP31 cells. Kisspeptin mRNA expression in GA28 cells, which expressed the oestrogen receptor alpha gene, was significantly reduced by 17 beta-oestradiol treatment. Furthermore, the transcriptional core promoter and repressive regions of the goat NKB gene were detected using GA28 cells. In conclusion, we have established goat KNDy and GnRH neurone cell lines that could be used to analyse molecular and cellular mechanisms regulating KNDy and GnRH neurones in vitro, facilitating the clarification of reproductive neuroendocrine mechanisms in ruminants.

    DOI: 10.1111/jne.12857

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  9. Peripheral administration of SB223412, a selective neurokinin-3 receptor antagonist, suppresses pulsatile luteinizing hormone secretion by acting on the gonadotropin-releasing hormone pulse generator in estrogen-treated ovariectomized female goats Reviewed

    Sasaki Takuya, Sonoda Tomoya, Tatebayashi Ryoki, Kitagawa Yuri, Oishi Shinya, Yamamoto Koki, Fujii Nobutaka, Inoue Naoko, Uenoyama Yoshihisa, Tsukamura Hiroko, Maeda Kei-ichiro, Matsuda Fuko, Morita Yasuhiro, Matsuyama Shuichi, Ohkura Satoshi

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   Vol. 66 ( 3 ) page: 351 - 357   2020.6

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  10. Colocalization of GPR120 and anterior pituitary hormone-producing cells in female Japanese Black cattle.

    Sho Nakamura, Kohei Noda, Masafumi Miwa, Shiori Minabe, Teruki Hagiwara, Akira Hirasawa, Shuichi Matsuyama, Ryutaro Moriyama

    The Journal of reproduction and development   Vol. 66 ( 2 ) page: 135 - 141   2020.4

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    Negative energy balance in domestic animals suppresses their reproductive function. These animals commonly use long-chain fatty acids (LCFAs) from adipocytes as an energy source under states of malnutrition. The G-protein coupled receptor, GPR120, is a specific receptor for LCFAs, but its role in reproductive function remains unknown in domestic animals. The purpose of this study was to examine whether GPR120 is involved in the reproductive system of cattle. GPR120 mRNA expression was evaluated in brain, pituitary, and ovarian tissue samples by RT-PCR. GPR120 gene expression was detected with high intensity only in the anterior pituitary sample, and GPR120-immunoreactive cells were found in the anterior pituitary gland. Double immunohistochemistry of GPR120 in the anterior pituitary hormone-producing cells, such as gonadotropes, thyrotropes, lactotropes, somatotropes, and corticotropes, was performed to clarify the distribution of GPR120 in the anterior pituitary gland of ovariectomized heifers. Luteinizing hormone β subunit (LHβ)- and follicle-stimulating hormone β subunit (FSHβ)-immunoreactive cells demonstrated GPR120 immunoreactivity at 80.7% and 85.9%, respectively. Thyrotropes, lactotropes, somatotropes, and corticotropes coexpressed GPR120 at 21.1%, 5.4%, 13.6%, and 14.5%, respectively. In conclusion, the present study suggests that GPR120 in the anterior pituitary gland might mediate LCFA signaling to regulate gonadotrope functions, such as hormone secretion or production, in cattle.

    DOI: 10.1262/jrd.2019-111

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  11. Colocalization of GPR120 and anterior pituitary hormone-producing cells in female Japanese Black cattle Reviewed

    Nakamura Sho, Noda Kohei, Miwa Masafumi, Minabe Shiori, Hagiwara Teruki, Hirasawa Akira, Matsuyama Shuichi, Moriyama Ryutaro

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   Vol. 66 ( 2 ) page: 135 - 141   2020.4

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  12. Establishment of long-term chronic recording technique of in vivo ovarian parenchymal temperature in Japanese Black cows.

    Yasuhiro Morita, Riho Ozaki, Akihisa Mukaiyama, Takuya Sasaki, Ryoki Tatebayashi, Ai Morishima, Yuri Kitagawa, Reika Suzumura, Ryoya Abe, Hiroko Tsukamura, Shuichi Matsuyama, Satoshi Ohkura

    The Journal of reproduction and development   Vol. 66 ( 3 ) page: 271 - 275   2020

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    The reproductive performance of cattle can be suppressed by heat stress. Reproductive organ temperature, especially ovarian temperature, may affect follicle development and ovulation. The establishment of a technique for long-term measurement of ovarian temperature could prove useful in understanding the mechanisms underlying the temperature-dependent changes in follicular development and subsequent ovulation in cows. Here we report a novel method facilitating long-term and continuous recording of ovarian parenchymal temperature in cows. The method revealed that the ovarian temperature in the luteal phase was constantly maintained lower than the vaginal temperature, and that the diurnal temperature variation in the ovary was significantly greater than that in the vagina, suggesting that the ovaries may require a lower temperature than other organs to maintain their functions. This novel method could be used for the further understanding of ovarian functions during estrous cycles in cows.

    DOI: 10.1262/jrd.2019-097

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  13. Prepartum change in ventral tail base surface temperature in beef cattle: comparison with vaginal temperature and behavior indices, and effect of ambient temperature

    MIWA Masafumi, MATSUYAMA Shuichi, NAKAMURA Sho, NODA Kohei, SAKATANI Miki

    Journal of Reproduction and Development   Vol. 65 ( 6 ) page: 515 - 525   2019.12

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    <p> Prediction of parturition is essential for sustainable production in beef and dairy cattle, yet the present methods are limited by their high invasiveness and low utility. Here we compared prepartum changes in ventral tail base surface temperature (ST) with changes in vaginal temperature (VT) and behavioral indices. We analyzed 22 parturitions from 22 beef cows. Changes in daily values of ST, VT, and behavioral indices over the 7 days before parturition were investigated. Hourly values were calculated as the actual values minus the mean values for the same hour over a 3-day period, and the changes in hourly values over the 48 h before parturition were investigated. To test the effect of ambient temperature, tested cows were assigned to two season-groups based on the ambient temperature to which they were exposed (warm: n = 13; cool: n = 9), and the daily and hourly values of the indices were compared between seasons. A decrease in ST occurred approximately 30 h before parturition, which was similar to the time of the decrease in VT and earlier than the increase of behavioral indices. In addition, a unique fluctuation of ST observed in the last few hours before parturition indicates that ST could provide a sign for parturition not only in the long-term like VT, but also in the short-term like behavioral indices. Although ST was more sensitive to ambient temperature than VT or the behavioral indices, the day of parturition could be predicted from ST in both the warm and cool seasons.</p>

    DOI: 10.1262/jrd.2019-087

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  14. Prepartum change in ventral tail base surface temperature in beef cattle: comparison with vaginal temperature and behavior indices, and effect of ambient temperature Reviewed

    Miwa Masafumi, Matsuyama Shuichi, Nakamura Sho, Noda Kohei, Sakatani Miki

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   Vol. 65 ( 6 ) page: 515 - 525   2019.12

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  15. ウシ主席卵胞および次席卵胞の顆粒層細胞において発現する遺伝子の網羅的解析

    大下 雪奈, 渡辺 雄貴, 春日 崇, 中平 陽子, 棟朝 亜理紗, 木村 康二, 松山 秀一, 大蔵 聡, 真方 文絵, 松田 二子

    日本獣医学会学術集会講演要旨集   Vol. 162回   page: 439 - 439   2019.8

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  16. Characterisation of bovine embryos following prolonged culture in embryonic stem cell medium containing leukaemia inhibitory factor. Reviewed International journal

    Misa Hosoe, Tadashi Furusawa, Ken-Go Hayashi, Toru Takahashi, Yutaka Hashiyada, Keiichiro Kizaki, Kazuyoshi Hashizume, Tomoyuki Tokunaga, Shuichi Matsuyama, Ryosuke Sakumoto

    Reproduction, fertility, and development   Vol. 31 ( 6 ) page: 1157 - 1165   2019.5

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    In order to help elucidate the process of epiblast and trophoblast cell differentiation in bovine embryos invitro, we attempted to develop a suitable culture medium to allow extended embryo culture. Day 7 bovine blastocysts developed in conventional medium were cultured further in embryonic stem cell medium with or without leukaemia inhibitory factor (LIF) until Day 23. At Day 14, the expression of octamer-binding transcription factor 3/4 (OCT3/4) and VIMENTIN was significantly higher in embryos cultured with than without LIF, but embryonic disc formation was not observed. Although expression of SRY (sex determining region Y)-box 17 (SOX17) mRNA was significantly lower in Day 14 embryos cultured with and without LIF than in invivo embryos, hypoblast cells formed just inside the trophoblast cells of the invitro-cultured embryos. On Day 23, expression of placental lactogen (PL) and prolactin-related protein 1 (PRP1) was not affected by LIF in invitro-cultured embryos, levels of both genes were significantly lower in the invitro than invivo embryos. Similar to invivo embryos, binucleate cell clusters seen in Day 23invitro-cultured embryos were composed of PL-negative and -positive cells. These results suggest that our culture system partially reproduced the differentiation process of trophoblast cells invivo.

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  17. ウシにおける心拍変動解析を用いた環境ストレスの評価

    森田 康広, 向山 晃永, 佐々木 拓弥, 尾崎 理穂, 舘林 亮輝, 北川 悠梨, 森島 愛, 阿部 良哉, 鈴村 玲香, 松山 秀一, 大蔵 聡

    日本畜産学会大会講演要旨集   Vol. 125回   page: 192 - 192   2019.3

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  18. ヤギのGnRHパルス生成活性に対するカルシトニン受容体シグナル伝達の役割(The role of calcitonin receptor signaling on the GnRH pulse generator activity in goats)

    北川 悠梨, 佐々木 拓弥, 森島 愛, 舘林 亮輝, 森田 康広, 松山 秀一, 井上 直子, 上野山 賀久, 束村 博子, 大蔵 聡

    日本畜産学会大会講演要旨集   Vol. 125回   page: 124 - 124   2019.3

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  19. Age-related changes in the bovine corpus luteum function and progesterone secretion. Reviewed

    Hori K, Matsuyama S, Nakamura S, Iwata H, Kuwayama T, Miyamoto A, Shirasuna K

    Reproduction in domestic animals = Zuchthygiene   Vol. 54 ( 1 ) page: 23 - 30   2019.1

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    DOI: 10.1111/rda.13303

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  20. Propionate and butyrate induce gene expression of monocarboxylate transporter 4 (MCT4) and cluster of differentiation 147 (CD147) in cultured rumen epithelial cells derived from pre-weaning dairy calves Reviewed International journal

    Sho Nakamura, Satoshi Haga, Koji Kimura, Shuichi Matsuyama

    Journal of Animal Science   Vol. 96 ( 11 ) page: 4902 - 4911   2018.11

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    Short-chain fatty acids (SCFAs) are the main source of energy for postweaning ruminants. The monocarboxylic acid transporters, MCT1 and MCT4, are thought to contribute to the absorption of SCFAs from the surface of the rumen following weaning. The present study measured changes in MCT1 and MCT4 expression in ruminal epithelial cells isolated from male preweaning (22 to 34 d old, n = 6) and postweaning (55 to 58 d old, n = 8) calves after euthanasia and sought to examine whether SCFAs stimulate the expression of these transporters. In the current study, cluster of differentiation 147 (CD147) gene expression in the rumen was also investigated since CD147 has been considered to act as ancillary protein for MCT1 and MCT4 to express their correct function. The gene expression levels of MCT1, MCT4, and CD147 in the rumen were found to be significantly higher in postweaning calves than in preweaning calves. Strong MCT1 immunoreactivity was detected in both the stratum basale (SB) and the stratum spinosum (SS) in postweaning ruminal epithelium. Expression of MCT1 in preweaning calves was localized to a specific region of the SB and of the SS. MCT4-immunopositive cells were detected in the stratum corneum (SC) of the ruminal epithelium in postweaning calves. However, only a low level of signal was detected in the SC of preweaning animals. Furthermore, in vitro experiments, ruminal epithelial cells were incubated for 24 h with acetate (0.04, 0.4, and 4 mM), propionate (0.2, 2, and 20 mM), butyrate (0.1, 1, and 10 mM), or β-hydroxybutyrate (BHBA; 0.1, 1, and 10 mM), respectively. Both propionate and butyrate induced an increase in the gene expression levels of MCT4 and CD147, but did not affect MCT1 gene expression. There are no significant effects of acetate and BHBA treatment on these gene expressions. Taken together, these results suggest that an increase in MCT4 and CD147 gene expression in the ruminal epithelium of postweaning calves is likely to be due to the effects of propionate and butyrate derived from a solid-based diet, which may contribute to ruminal development following weaning.

    DOI: 10.1093/jas/sky334

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  21. Publisher Correction: Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle. Reviewed

    Ikeda M, Matsuyama S, Akagi S, Ohkoshi K, Nakamura S, Minabe S, Kimura K, Hosoe M

    Scientific reports   Vol. 8 ( 1 ) page: 1470   2018.1

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    DOI: 10.1038/s41598-018-19725-z

  22. Correction of a Disease Mutation using CRISPR/Cas9-assisted Genome Editing in Japanese Black Cattle Reviewed

    Mitsumi Ikeda, Shuichi Matsuyama, Satoshi Akagi, Katsuhiro Ohkoshi, Sho Nakamura, Shiori Minabe, Koji Kimura, Misa Hosoe

    SCIENTIFIC REPORTS   Vol. 7 ( 1 ) page: 17827   2017.12

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    Isoleucyl-tRNA synthetase (IARS) syndrome is a recessive disease of Japanese Black cattle caused by a single nucleotide substitution. To repair the mutated IARS gene, we designed clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated protein 9 (Cas9) to create a double-strand break near the mutation site. CRISPR/Cas9 and donor DNA that contained a synonymous codon for the correct amino acid and an Aequorea coerulescens Green Fluorescent Protein (AcGFP) cassette with a piggyBac transposase recognition site at both ends were introduced into bovine fetal fibroblast (BFF) cells isolated from a homozygous mutant calf. Recombinant cells were enriched on the basis of expression of AcGFP, and two cell lines that contained the repaired allele were subcloned. We generated somatic cell nuclear transfer (SCNT) embryos from the repaired cells and transferred 22 blastocysts to recipient cows. In total, five viable fetuses were retrieved at Days 34 and 36. PiggyBac transposase mRNA was introduced into BFF cells isolated from cloned foetuses and AcGFP-negative cells were used for second round of cloning. We transferred nine SCNT embryos to recipient cows and retrieved two fetuses at Day 34. Fetal genomic DNA analysis showed correct repair of the IARS mutation without any additional DNA footprint.

    DOI: 10.1038/s41598-017-17968-w

    Web of Science

    PubMed

  23. Production of calves by the transfer of cryopreserved bovine elongating conceptuses and possible application for preimplantation genomic selection Reviewed

    Takashi Fujii, Hiroki Hirayama, Akira Naito, Masashi Kashima, Hitomi Sakai, Shigeo Fukuda, Hitomi Yoshino, Satoru Moriyasu, Soichi Kageyama, Yoshikazu Sugimoto, Shuichi Matsuyama, Hiroyuki Hayakawa, Koji Kimura

    JOURNAL OF REPRODUCTION AND DEVELOPMENT   Vol. 63 ( 5 ) page: 497 - 504   2017.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SOCIETY REPRODUCTION & DEVELOPMENT-SRD  

    Preimplantation genomic selection based on single nucleotide polymorphism (SNP) genotypes is expected to accelerate genetic improvement in cattle. However, genome-wide genotyping at the early embryonic stage has several limitations, such as the technical difficulty of embryonic biopsy and low accuracy of genotyping resulting from a limited number of biopsied cells. After hatching from the zona pellucida, the morphology of the bovine embryo changes from spherical to filamentous, in a process known as elongation. The bovine nonsurgical elongating conceptus transfer technique was recently developed and applied for sexing without requiring specialized skills for biopsy. In order to develop a bovine preimplantation genomic selection system combined with the elongating conceptus transfer technique, we examined the accuracy of genotyping by SNP chip analysis using the DNA from elongating conceptuses (Experiment 1) and optimal cryopreservation methods for elongating conceptuses (Experiment 2). In Experiment 1, the call rates of SNP chip analysis following whole genome amplification in biopsied cells from two elongating conceptuses were 95.14% and 99.32%, which were sufficient for estimating genomic breeding value. In Experiment 2, the rates of dead cells in elongating conceptuses cryopreserved by slow freezing were comparable to those in fresh elongating conceptuses. In addition, we obtained healthy calves by the transfer of elongating conceptuses cryopreserved by slow freezing. Our findings indicate that the elongating conceptus transfer technology enables preimplantation genomic selection in cattle based on SNP chip analysis. Further studies on the optimization of cryopreservation methods for elongating conceptuses are required for practical application of the selection system.

    DOI: 10.1262/jrd.2017-025

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    PubMed

  24. Interferon Tau Regulates Cytokine Production and Cellular Function in Human Trophoblast Cell Line Reviewed

    Nao Tanikawa, Kotomi Seno, Ryouka Kawahara-Miki, Koji Kimura, Shuichi Matsuyama, Hisataka Iwata, Takehito Kuwayama, Koumei Shirasuna

    JOURNAL OF INTERFERON AND CYTOKINE RESEARCH   Vol. 37 ( 10 ) page: 456 - 466   2017.10

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:MARY ANN LIEBERT, INC  

    Type I interferons (IFN), including IFN-beta (IFNB), activate multiple STAT signaling to drive various biological responses. Another type I IFN, IFN-tau (IFNT), secreted by ruminant embryonic trophoblast cells, has multiple functions with low cytotoxicity. Here, we examined the effects of IFNT on human trophoblast cell functions. First, we performed next-generation sequencing and demonstrated that IFNT-dependent changes in the human Sw.71 trophoblast cell line are partly mediated by proinflammatory as well as IFN signaling. Next, we validated candidate genes, and data confirmed that IFNT stimulated interleukin-6 (IL-6) and IL-8 mRNA expression and secretion. However, human IFNB did not affect IL-6 and IL-8 mRNA expression and secretion. IFNT-induced cytokine secretion was dependent on STAT3 signaling, but not STAT1 signaling. In addition, treatment with IFNT, IL-6, or IL-8 increased cell proliferation, and IFNT also stimulated cell migration in human trophoblast cells. Although IFNT did not affect superoxide dismutase (SOD) 1 mRNA expression, it clearly increased mitochondrial SOD2 mRNA expression, resulting in the acceleration of SOD activity. We demonstrated that in addition to IFN signaling, IFNT also regulated inflammation-related signaling as well as cell proliferation, migration, and redox signaling in human trophoblast cells.

    DOI: 10.1089/jir.2017.0057

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    PubMed

  25. Age-associated mRNA expression changes in bovine endometrial cells in vitro Reviewed

    Nao Tanikawa, Ayaka Ohtsu, Ryouka Kawahara-Miki, Koji Kimura, Shuichi Matsuyama, Hisataka Iwata, Takehito Kuwayama, Koumei Shirasuna

    REPRODUCTIVE BIOLOGY AND ENDOCRINOLOGY   Vol. 15 ( 1 ) page: 63   2017.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:BIOMED CENTRAL LTD  

    Background: Endometrial cells secrete various cytokines and the dysfunction of endometrial cells may directly lead to infertility. Interferon tau (IFNT) secreted by trophoblast cells, a well-known pregnancy recognition signal in ruminants, acts on the uterus to prepare for pregnancy. Aging causes cellular and organ dysfunction, and advanced maternal age is associated with reduced fertility. However, few studies have investigated age-dependent changes in the uterus.
    Methods: Using next generation sequencing and real-time PCR, we examined mRNA expression in bovine endometrial cells in vitro obtained from young (mean 45.2 months) and aged (mean 173.5 months) animals and the effects of IFNT depending on the age.
    Results: We showed that inflammation-related (predicted molecules are IL1A, C1Qs, DDX58, NFKB, and CCL5) and interferon-signaling (predicted molecules are IRFs, IFITs, STATs, and IFNs) pathways were activated in endometrial cells obtained from aged compared to young cows. Also, the activation of "DNA damage checkpoint regulation" and the inhibition of "mitotic mechanisms" in endometrial cells obtained from aged cows were evident. Moreover, we showed lower cell viability levels in endometrial cells obtained from aged compared to young cows. Although treatment with IFNT upregulated various types of interferon stimulated genes both in endometrial cells obtained from young and aged cows, the rate of increase by IFNT stimulus was obviously lower in endometrial cells obtained from aged compared to young cows.
    Conclusions: Endometrial cells obtained from aged cows exhibited higher levels of inflammatory- and IFN-signaling, and dysfunction of cell division compared with young cows. In addition, a high basal level of IFN-related genes in endometrial cells of aged cows is suggested a concept of "inflammaging".

    DOI: 10.1186/s12958-017-0284-z

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    PubMed

  26. A neurokinin 3 receptor-selective agonist accelerates pulsatile luteinizing hormone secretion in lactating cattle Reviewed

    Sho Nakamura, Yoshihiro Wakabayashi, Takashi Yamamura, Satoshi Ohkura, Shuichi Matsuyama

    BIOLOGY OF REPRODUCTION   Vol. 97 ( 1 ) page: 81 - 90   2017.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS INC  

    Pulsatile gonadotropin-releasing hormone (GnRH) secretion, which is indispensable for follicular development, is suppressed in lactating dairy and beef cattle. Neurokinin B (NKB) neurons in the arcuate nucleus of the hypothalamus are considered to play an essential role in generating the pulsatile mode of GnRH/luteinizing hormone (LH) secretion. The present study aimed to clarify the role of NKB-neurokinin 3 receptor (NK3R) signaling in the pulsatile pattern of GnRH/gonadotropin secretion in postpartum lactating cattle. We examined the effects of the administration of an NK3R-selective agonist, senktide, on gonadotropin secretion in lactating cattle. The lactating cattle, at approximately 7 days postpartum, were intravenously infused with senktide (30 or 300 nmol/min) or vehicle for 24 h. The administration of 30 or 300 nmol/min senktide significantly increased LH pulse frequency compared to in the control group during 0-4 or 20-24 h after infusion, respectively. Moreover, LH and follicle-stimulating hormone levels were gradually increased by 300 nmol/min administration of senktide during the 0-4-h sampling period. Ultrasonography of the ovaries was performed to identify the first postpartum ovulation in senktide-administered lactating cattle. The interval from calving to first postpartum ovulation was significantly shorter in the 300 nmol/min senktide-administered group than in the control group. Taken together, these findings suggest that senktide infusion elicits an increase in LH pulse frequency that may stimulate follicular development and, in turn, induce the first postpartum ovulation in lactating cattle.
    Summary Sentence
    Administration of senktide, an NK3R-selective agonist, elicits an increase in LH pulse frequency that may stimulate follicular development and, in turn, induce the first postpartum ovulation in lactating cattle.

    DOI: 10.1093/biolre/iox068

    Web of Science

    Scopus

    PubMed

  27. ウシの子宮機能と受胎性について Reviewed

    木村康二、松山秀一

    家畜感染症学会誌   Vol. 5 ( 4 ) page: 145 - 151   2016.12

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  28. Derivation of Induced Trophoblast Cell Lines in Cattle by Doxycycline-Inducible piggyBac Vectors Reviewed

    Kawaguchi T, Cho D, Hayashi M, Tsukiyama T, Kimura K, Matsuyama S, Minami N, Yamada M, Imai H.

    PLoS ONE   Vol. 11 ( 12 ) page: e0167550   2016.11

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  29. Immunohistochemical characterization of the arcuate kisspeptin/neurokinin B/dynorphin (KNDy) and preoptic kisspeptin neuronal populations in the hypothalamus during the estrous cycle in heifers. Reviewed

    Hassaneen A, Naniwa Y, Suetomi Y, Matsuyama S, Kimura K, Ieda N, Inoue N, Uenoyama Y, Tsukamura H, Maeda K, Matsuda F, Ohkura S.

    Journal of Reproduction and Development   Vol. 62 ( 5 ) page: 471 - 477   2016.10

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  30. ウシにおける胚の生存性と子宮環境 Reviewed

    松山秀一、中村翔、木村康二

    日本胚移植学雑誌   Vol. 38 ( 3 ) page: 115 - 121   2016.9

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    Authorship:Lead author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  31. Possible role of IFNT on the bovine corpus luteum and neutrophils during the early pregnancy. Reviewed

    Shirasuna K, Matsumoto H, Matsuyama S, Kimura K, Bollwein H, Miyamoto A.

    Reproduction   Vol. 150 ( 3 ) page: 217 - 225   2015.9

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  32. Generation of Naive Bovine Induced Pluripotent Stem Cells Using PiggyBac Transposition of Doxycycline-Inducible Transcription Factors. Reviewed

    Kawaguchi T, Tsukiyama T, Kimura K, Matsuyama S, Minami N, Yamada M, Imai H.

    PLoS ONE   Vol. 10 ( 8 ) page: e0135403   2015.8

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  33. Interferon-tau attenuates uptake of nanoparticles and secretion of interleukin-1β in macrophages. Reviewed

    Hara K, Shirasuna K, Usui F, Karasawa T, Mizushina Y, Kimura H, Kawashima A, Ohkuchi A, Matsuyama S, Kimura K, Takahashi M.

    PLoS ONE   Vol. 9 ( 12 ) page: e113974   2014.12

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  34. Regulation of gonadotropin secretion by monitoring energy availability. Reviewed

    Matsuyama S, Kimura K

    Reproductive Medicine and Biology   Vol. 14 ( 2 ) page: 39 - 47   2014.9

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  35. Successful nonsurgical transfer of bovine elongating conceptuses and its application to sexing. Reviewed

    Kimura K Matsuyama S.

    Journal of Reproduction and Development   Vol. 60 ( 3 ) page: 210 - 215   2014.7

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  36. Aerococcus vaginalis sp. nov., isolated from the vaginal mucosa of a beef cow, and emended descriptions of Aerococcus suis, Aerococcus viridans, Aerococcus urinaeequi, Aerococcus urinaehominis, Aerococcus urinae, Aerococcus christensenii and Aerococcus sanguinicola. Reviewed

    Tohno M, Kitahara M, Matsuyama S, Kimura K, Ohkura M, Tajima K.

    International Journal of Systematic and Evolutionary Microbiology   Vol. 64   page: 1229 - 1236   2014.4

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  37. 末梢単核球を利用したウシ胚の生存性モニタリング-妊娠早期における胚死滅のメカニズム解明に向けて- Reviewed

    松山秀一、木村康二

    日本胚移植学雑誌   Vol. 35 ( 3 ) page: 123 - 127   2013.9

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  38. Characteristics of bovine inner cell mass-derived cell lines and their fate in chimeric conceptuses. Reviewed

    Furusawa T, Ohkoshi K, Kimura K, Matsuyama S, Akagi S, Kaneda M, Ikeda M, Hosoe M, Kizaki K, Tokunaga T.

    Biology of Reproduction   Vol. 89 ( 2 ) page: 1 - 12   2013.8

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  39. Sexual dimorphism during early embryonic development in mammals. Reviewed

    Kimura K, Matsuyama S.

    J. Mamm. Ova Res.   Vol. 29   page: 103 - 112   2012.11

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  40. Relationship between plasma progesterone concentration and number of conceptuses and their growth in superovulated cattle. Reviewed

    Matsuyama S, Sakaguchi Y, Kimura K.

    Journal of Reproduction and Development   Vol. 58 ( 5 ) page: 609 - 614   2012.11

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  41. Relationship between quantity of IFNT estimated by IFN-stimulated gene expression in peripheral blood mononuclear cells and bovine embryonic mortality after AI or ET. Reviewed

    Matsuyama S, Kojima T, Kato S, Kimura K.

    Reproductive Biology and Endocrinology   Vol. 10   page: 21   2012.3

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  42. Possible involvement of IFNT in lymphangiogenesis in the corpus luteum during the maternal recognition period in the cow. Reviewed

    Nitta A, Shirasuna K, Haneda S, Matsui M, Shimizu T, Matsuyama S, Kimura K, Bollwein H, Miyamoto A.

    Reproduction   Vol. 142 ( 6 ) page: 79 - 92   2011.12

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  43. Morphological Evidence for Direct Interaction between Kisspeptin and Gonadotropin-Releasing Hormone Neurons at the Median Eminence of the Male Goat: An Immunoelectron Microscopic Study. Reviewed

    Matsuyama S, Ohkura S, Mogi K, Wakabayashi Y, Mori Y, Tsukamura H, Maeda K, Ichikawa M, Okamura H.

    Neuroendocrinology   Vol. 94 ( 4 ) page: 323 - 332   2011.11

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  44. Gonadotrophin-releasing hormone pulse generator activity in the hypothalamus of the goat. Reviewed

    Ohkura S, Takase K, Matsuyama S, Mogi K, Ichimaru T, Wakabayashi Y, Uenoyama Y, Mori Y, Steiner RA, Tsukamura H, Maeda K, Okamura H.

    Journal of Neuroendocrinology   Vol. 21 ( 10 ) page: 813 - 821   2009.10

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  45. Food deprivation induces monocarboxylate transporter 2 expression in the brainstem in female rat. Reviewed

    Matsuyama S, Ohkura S, Iwata K, Uenoyama Y, Tsukamura H, Maeda K, Kimura K.

    Journal of Reproduction and Development   Vol. 55 ( 3 ) page: 256 - 261   2009.7

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  46. Central Administration of Neuropeptide B, but not Prolactin-Releasing Peptide, Stimulates Cortisol Secretion in Sheep. Reviewed

    Mogi K, Ito S, Matsuyama S, Ohara H, Sakumoto R, Yayo K , Ohkura S, Sutoh M、Mori Y, Okamura H.

    Journal of Reproduction and Development   Vol. 54 ( 2 ) page: 138 - 141   2008.4

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  47. 既知個体および自身の映像がホルスタイン種去勢牛の隔離ストレス反応に及ぼす影響 Reviewed

    伊藤秀一、矢用健一、松山秀一、森山隆太郎、須藤まどか、粕谷悦子、大蔵聡、岡村裕昭

    応用動物行動学会報(Animal Behaviour and Management)   Vol. 42 ( 4 ) page: 203 - 208   2006.12

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  48. Acute lipoprivation suppresses pulsatile luteinizing hormone secretion without affecting food intake in female rats. Reviewed

    Shahab M, Sajapitak S, Tsukamura H, Kinoshita M, Matsuyama S, Ohkura S, Yamada S, Uenoyama Y, I’Anson H, Maeda K

    Journal of Reproduction and Development   Vol. 52 ( 6 ) page: 763 - 772   2006.12

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  49. Activation of melanocortin receptors accelerates the gonadotropin-releasing hormone pulse generator activity in goats. Reviewed

    Matsuyama S, Ohkura S, Sakurai K, Tsukamura H, Maeda K, Okamura H.

    Neuroscience Letters   Vol. 383 ( 3 ) page: 289 - 294   2005.8

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  50. Simultaneous observation of the GnRH pulse generator activity and plasma concentrations of metabolites and insulin during fasting and subsequent refeeding periods in Shiba goats. Reviewed

    Matsuyama S, Ohkura S, Ichimaru T, Sakurai K, Tsukamura H, Maeda K, Okamura H.

    Journal of Reproduction and Development   Vol. 50 ( 6 ) page: 697 - 704   2005.1

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  51. Further evidence for the role of glucose as a metabolic regulator of hypothalamic gonadotropin-releasing hormone pulse generator activity in goats. Reviewed

    Ohkura S, Ichimaru T, Ito F, Matsuyama S, Okamura H.

    Endocrinology   Vol. 145 ( 7 ) page: 3239 - 3246   2004.7

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  52. Body growth and plasma concentrations of metabolites and metabolic hormones during the pubertal period in female Shiba goats. Reviewed

    Sakurai K, Ohkura S, Matsuyama S, Kato K, Obara Y, Okamura H.

    Journal of Reproduction and Development   Vol. 50 ( 2 ) page: 197 - 205   2004.4

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  53. Central cholecystokinin-octapeptide accelerates the activity of the hypothalamic gonadotropin-releasing hormone pulse generator in goats. Reviewed

    Ichimaru T, Matsuyama S, Ohkura S, Mori Y, Okamura H.

    Journal of Neuroendocrinology   Vol. 15 ( 1 ) page: 80 - 86   2003.1

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▼display all

MISC 4

  1. Improvement of post-partum reproductive performance by Rumen protected niacin supplementation in cattle

    森田康広, 松山秀一

    食肉に関する助成研究調査成果報告書   Vol. 39   2021

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  2. 雌ヤギ由来KNDyニューロンおよびGnRHニューロン不死化細胞株の樹立

    松田二子, 末富祐太, 舘林亮輝, 園田修平, 棟朝亜理紗, 松山秀一, 井上直子, 上野山賀久, 武内ゆかり, 束村博子, 大蔵聡

    日本内分泌学会雑誌   Vol. 96 ( 4 (Web) )   2020

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  3. シバヤギのGnRHパルス発生メカニズムにおけるセロトニンの役割

    森島愛, 佐々木拓弥, 舘林亮輝, 北川悠梨, 森田康広, 松山秀一, 井上直子, 上野山賀久, 束村博子, 大蔵聡

    日本下垂体研究会学術集会プログラム・講演要旨集   Vol. 33rd   2018

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  4. シバヤギにおけるパルス状GnRH分泌調節メカニズムに対するアミリンの作用

    北川悠梨, 佐々木拓弥, 森島愛, 舘林亮輝, 森田康広, 松山秀一, 井上直子, 上野山賀久, 束村博子, 大蔵聡

    日本下垂体研究会学術集会プログラム・講演要旨集   Vol. 33rd   2018

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Presentations 35

  1. ウシ過剰排卵処置時におけるニューロキニンB受容体作動薬の静脈内投与が回収胚の発育ステージおよび遺伝子発現に及ぼす影響

    松山秀一、中村 翔、美辺詩織、大石真也、大蔵 聡、木村康二

    日本受精卵移植関連合同研究会東京大会 第32回東日本家畜受精卵移植技術研究会大会、第23回日本胚移植研究会大会  2016.9 

  2. 泌乳期ウシにおけるニューロキニンB受容体作動薬が黄体形成ホルモンのパルス状分泌に及ぼす影響

    中村 翔、若林嘉浩、山村 崇、大蔵 聡、松山秀一

    日本下垂体研究会  2017.8 

  3. ウシ過剰排卵処置時におけるニューロキニンB受容体作動薬の末梢投与が黄体形成ホルモン分泌および回収胚の品質に及ぼす影響

    中村 翔, 木村 康二, 美辺 詩織, 大石 真也, 大蔵 聡, 松山 秀一

    第110回日本繁殖生物学会大会  2016.9 

  4. ニューロキニンB受容体作動薬投与が泌乳期ウシの分娩後初回排卵へ及ぼす影響

    中村 翔、若林嘉浩、山村 崇、大蔵 聡、松山秀一

    日本受精卵移植関連合同研究会京都大会(第24回日本胚移植研究会大会、第33回東日本家畜受精卵移植技術研究会大会)  2017.9 

  5. ウシ子宮内膜におけるミトコンドリア機能の亢進は受胎率を飛躍的に向上させる

    松山秀一、中村 翔、美辺詩織、岩田尚孝、木村康二

    日本受精卵移植関連合同研究会京都大会(第24回日本胚移植研究会大会、第33回東日本家畜受精卵移植技術研究会大会)  2017.9 

  6. 着床前ゲノム選抜技術開発に向けたウシ伸長胚保存法の検討

    藤井 貴志, 平山 博樹, 陰山 聡一, 内藤 学, 福田 茂夫, 吉野 仁美, 松山 秀一, 木村 康二, 早川 宏之, 津田 栄, 森安 悟

    第110回日本繁殖生物学会大会  2016.9 

  7. ニューロキニンB受容体作動薬投与が夏季のウシ体温に及ぼす影響

    中村翔, 三輪雅史, 森田康広, 山村崇, 若林嘉浩, 大蔵聡, 松山秀一

    第112回日本繁殖生物学会大会  2019.9 

  8. 低受胎牛の子宮環境と改善技術 Invited

    松山秀一

    第3回日本胚移植技術研究会和歌山大会 シンポジウム「畜産領域におけるリキッドバイオプシーの展開」  2019.8 

  9. Understanding the uterine environment in subfertile cattle Invited

    MATSUYAMA S, NAKAMURA S, OHKURA S, KIMURA K

    2019.9 

  10. ウシ子宮内膜Side population細胞における幹細胞マーカーの発現解析

    館林亮輝、中村翔、美辺詩織、古澤軌、阿部良哉、森田康広、大蔵聡、木村康二、松山秀一

    第112回日本繁殖生物学会大会  2019.9 

  11. パルス状GnRH分泌制御機構におけるカルシトニン受容体の役割

    北川 悠梨, 佐々木 拓弥, 森島 愛, 舘林 亮輝, 森田 康広, 松山 秀一, 井上 直子, 上野山 賀久, 束村 博子, 大蔵 聡

    第112回日本繁殖生物学会大会  2019.9 

  12. シバヤギのパルス状GnRH分泌制御メカニズムにおけるセロトニンの役割

    佐々木 拓弥, 森島 愛, 中西 真莉菜, 鈴村 玲香, 舘林 亮輝, 北川 悠梨, 森田 康広, 松山 秀一, 井上 直子, 上野山 賀久, 束村 博子, 大蔵 聡

    第112回日本繁殖生物学会大会  2019.9 

  13. 長鎖脂肪酸への曝露がウシおよびブタ精子の運動性に与える影響

    森山 隆太郎, 平井 直明, 野田 航平, 山口 直哉, 三輪 雅史, 中村 翔, 松山 秀一

    第111回日本繁殖生物学会大会  2018.9 

  14. 体表温センサによる肉用繁殖牛の分娩時刻予知の検討

    三輪 雅史, 中村 翔, 松山 秀一

    第111回日本繁殖生物学会大会  2018.9 

  15. ニューロキニンB受容体拮抗剤の経口投与はパルス状LH分泌を抑制する

    佐々木 拓弥, 園田 朋也, 大石 真也, 藤井 信孝, 森田 康広, 松山 秀一, 井上 直子, 上野山 賀久, 束村 博子, 前多 敬一郎, 松田 二子, 大蔵 聡

    第111回日本繁殖生物学会大会  2018.9 

  16. ニューロキニン作動薬の持続投与がウシの性腺刺激ホルモン分泌に及ぼす影響

    山村 崇, 松山 秀一, 中村 翔, 遠藤 なつ美, 田中 知己, 若林 嘉浩

    第111回日本繁殖生物学会大会  2018.9 

  17. ニューロキニンB受容体作動薬投与がウシの体温に及ぼす影響

    中村 翔, 三輪 雅史, 山村 崇, 若林 嘉浩, 松山 秀一

    第111回日本繁殖生物学会大会  2018.9 

  18. Effect of aging on the corpus luteum function in beef and dairy cows

    Konomi Hori, Shuichi Matsuyama, Sho Nakamura, Aoba Naito, Mayumi Matsushita, Wakana Arihara, Haruka Kawahara, Takehito Kuwayama, Hisataka Iwata, Akio Miyamoto and Koumei Shirasuna

    2017.9 

  19. Inflammation, interferon signaling, and cell cycle-related genes are changed with aging in bovine endometrial cells

    Nao Tanikawa, Ayaka Ohtsu, Ryoka Kawahara-Miki, Koji Kimura, Shuichi Matsuyama, Hisataka Iwata, Takehito Kuwayama and Koumei Shirasuna

    2017.9 

  20. Mitochondrial malfunction in the endometrium of subfertile cattle

    Shuichi Matsuyama, Sho Nakamura, Shiori Minabe and Koji Kimura

    2017.9 

  21. Establishment of immortalized GnRH neuronal cell lines derived from the cattle brain

    Riho OZAKI, Yoshihisa Uenoyama, Yuta Suetomi, Shuichi Matsuyama, Koji Kimura, Nahoko Ieda, Naoko Inoue, Hiroko Tsukamura, Satoshi Ohkura and Fuko Matsuda

    2017.9 

  22. A Neurokinin 3 Receptor-Selective Agonist, Senktide, Stimulates Pulsatile Luteinizing Hormone Secretion in Lactating Cattle

    Sho Nakamura, Yoshihiro Wakabayashi, Takashi Yamamura, Satoshi Ohkura and Shuichi Matsuyama

    2017.9 

  23. 分娩後のウシ子宮内膜におけるSide population細胞の発現挙動

    松山 秀一, 美辺 詩織, 中村 翔, 古澤 軌, 池田 光美, 木村 康二

    第110回日本繁殖生物学会大会  2016.9 

  24. ウシ伸長胚を用いた着床前ゲノム選抜技術開発に向けたSNP解析精度の検討

    平山 博樹, 内藤 学, 鹿島 聖志, 藤井 貴志, 陰山 聡一, 坂井 ひとみ, 杉本 喜憲, 松山 秀一, 木村 康二, 早川 宏之, 森安 悟

    第110回日本繁殖生物学会大会  2016.9 

  25. シバヤギにおけるGnRHパルス発生中枢の制御に関わるセロトニン受容体サブタイプの検討

    鈴村 玲香, 森田 康広, 松山 秀一, 佐々木 拓弥, 北川 悠梨, 井上 直子, 上野山 賀久, 束村 博子, 大蔵 聡

    第113回日本繁殖生物学会大会  2020.9 

  26. ウシ子宮内膜side population(SP)細胞の遺伝子発現解析と分娩後におけるSP細胞割合の推移

    舘林 亮輝, 中村 翔, 美辺 詩織, 古澤 軌, 阿部 良哉, 加治佐 実希, 森田 康広, 大蔵 聡, 木村 康二, 松山 秀一

    第113回日本繁殖生物学会大会  2020.9 

  27. メチルグリオキサールがウシ子宮内膜細胞に及ぼす影響

    阿部 良哉, 舘林 亮輝, 加治佐 実希, 森田 康広, 大蔵 聡, 松山 秀一

    第113回日本繁殖生物学会大会  2020.9 

  28. 分娩後のウシ子宮内膜における老化細胞の蓄積レベルの変化

    加治佐 実希, 舘林 亮輝, 阿部 良哉, 森田 康広, 大蔵 聡, 松山 秀一

    第113回日本繁殖生物学会大会  2020.9 

  29. 暑熱ストレス下におけるウシの排卵不全メカニズムの解明

    向山 晃永, 館林 亮輝, 北川 悠梨, 阿部 良哉, 鈴村 玲香, 松山 秀一, 大蔵 聡, 森田 康広

    第113回日本繁殖生物学会大会  2020.9 

  30. ポビドンヨード子宮内投与後のウシ子宮内膜における遺伝子発現変化およびside population細胞の変遷

    舘林亮輝,中村 翔,北川悠梨,加治佐実希,市川 怜,森田康広,大蔵 聡,木村康二,松山秀一

    第114回日本繁殖生物学会大会  2021.9 

  31. Kiss1ノックアウトヤギを用いたパルス状性腺刺激ホルモン分泌制御機構の解析

    近藤由梨,森田康広,松山秀一,稲谷結花,長尾勇佑,大蔵 聡

    令和3年度愛知県農学系4機関研究交流会  2021.12 

  32. ウシにおける呼吸音解析による熱中症の早期発見

    井上清詞,萬井裕己,北川悠梨,松山秀一,大蔵 聡,森田康広

    令和3年度愛知県農学系4機関研究交流会  2021.12 

  33. ウシ子宮腔内エクソソームに含まれるmiRNAが受胎性に及ぼす影響

    市川 怜,加治佐実希,大蔵 聡,森田康広,松山秀一

    令和3年度愛知県農学系4機関研究交流会  2021.12 

  34. 周産期の牛子宮内膜における老化細胞の蓄積レベルの変化

    加治佐実希,舘林亮輝,森田康広,大蔵 聡,松山秀一

    令和3年度東海畜産学会大会  2021.12 

  35. ルーメンバイパスナイアシン給与が分娩後のウシにおける子宮への血流変化及び子宮修復に与える影響

    萬井裕己,向山晃永,井上清詞,北川悠梨,松山秀一,大蔵 聡,森田康広

    第114回日本繁殖生物学会大会  2021.9 

▼display all

Research Project for Joint Research, Competitive Funding, etc. 5

  1. レスベラトロール子宮内投与による人工授精受胎率向上技術の開発

    2019.9 - 2020.3

    大垣共立銀行  第6回 OKBアグリビジネス助成金 

    松山秀一

  2. AIを活用した家畜疾病の早期発見技術の開発

    2017.4

    農林水産省  革新的技術開発・緊急展開事業(うち人工知能未来創造プロジェクト) 

    松山秀一

  3. ゲノム編集による家畜系統造成の加速化

    2015.4 - 2018.3

    農林水産省  農林水産業・食品産業科学技術研究推進事業 

    松山秀一

  4. 生体センシング技術を活用した次世代精密家畜個体管理システムの開発

    2014.4 - 2017.3

    内閣府  戦略的イノベーション創造プログラム(次世代農林水産業創造技術) 

    松山秀一

  5. 繁殖サイクルの短縮や受胎率向上のための技術開発

    2012.4 - 2017.3

    農林水産省  委託プロジェクト研究 

    松山秀一

KAKENHI (Grants-in-Aid for Scientific Research) 11

  1. 家畜の繁殖制御技術開発に資する卵胞発育中枢の神経メカニズムの解明

    Grant number:22H00397  2022.4 - 2027.3

    科学研究費助成事業  基盤研究(A)

    大蔵 聡, 若林 嘉浩, 松山 秀一

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    Authorship:Coinvestigator(s) 

    本研究では、家畜の繁殖機能制御の最上位中枢である卵胞発育制御中枢「性腺刺激ホルモン放出ホルモン(GnRH)パルスジェネレーター」に着目し、GnRHパルスジェネレーターがパルス状GnRH分泌を制御する神経メカニズムを解明する。ターゲットトキシンの弓状核内局所投与と投与部位近傍の神経活動の同時解析法や、オプトジェネティクス技術により、GnRHパルスジェネレーター本体のメカニズムを解明するとともに、ニューロキニンBおよびダイノルフィンAがGnRHパルスジェネレーターを調節するメカニズムを明らかにする。

  2. 家畜の繁殖制御技術開発に資する卵胞発育中枢の神経メカニズムの解明

    Grant number:22486583  2022.4 - 2026.3

    基盤研究(A)

    大蔵 聡, 松山 秀一, 若林嘉浩

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    Authorship:Coinvestigator(s) 

  3. ゲノム編集ヤギを活用したコンディショナルノックアウトによる排卵制御中枢の解明

    Grant number:21K19187  2021.7 - 2024.3

    科学研究費助成事業  挑戦的研究(萌芽)

    大蔵 聡, 松山 秀一, 森田 康広

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    Authorship:Coinvestigator(s) 

    本研究では、ヤギをウシのモデル動物として用い、ヤギ受精卵におけるゲノム編集技術により作出したゲノム編集ヤギを用いてキスペプチン遺伝子を視床下部内局所でノックアウトし、卵胞嚢腫の病態モデルを作出することに挑戦する。ウシの受胎率は年々低下しており、畜産現場において解決すべき喫緊の課題である。受胎率低下の要因のひとつである卵胞嚢腫は、多くの卵胞が排卵することなく卵巣に存在することから、排卵を調節する視床下部神経機構の機能不全が原因と想定される。本研究では、ゲノム編集ヤギを活用した卵胞嚢腫病態モデルにより、家畜の卵胞嚢腫発症の分子機構、ひいては排卵制御中枢のメカニズムを明らかにすることをめざす。

  4. Development of technique to decrease voluntary waiting period of cattle using ozone gel

    Grant number:20K21372  2020.7 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

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    Authorship:Coinvestigator(s) 

  5. 最終糖化産物がウシ子宮、卵管および胚発育に及ぼす影響とその作用機序の解明

    Grant number:20H03129  2020.4 - 2024.3

    科学研究費助成事業  基盤研究(B)

    松山 秀一, 木村 康二

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    Authorship:Principal investigator 

    Grant amount:\17680000 ( Direct Cost: \13600000 、 Indirect Cost:\4080000 )

    分娩後の乳牛では、泌乳にともなう負のエネルギーバランスを補うように設計された穀物主体の飼料が給与されているが、受胎率は低下の一途を辿っている。本研究では、高穀物含量の飼料によって産生されるメチルグリオキサールが受胎率低下の一因となっていることを検証する。ウシ生体を用いて、血中メチルグリオキサール濃度の上昇が受胎性に及ぼす影響を明らかにし、次いで、ウシ子宮内膜細胞、卵管細胞、卵子および胚を用いて、メチルグリオキサールが低受胎を引き起こすメカニズムを解明する。
    本研究では、高穀物含量の飼料によって産生されるメチルグリオキサール(MGO)が乳牛における受胎率低下の一因となっていることを検証する。まず、血中MGOが牛の受胎性に及ぼす影響を明らかにするため、ホルスタイン経産牛(n=110)の血中MGO濃度、月齢およびBCSと受胎成績との関係についてロジスティック回帰分析により検討した。その結果、人工授精時の血中MGO濃度と受胎成績に直接的な関係は認められなかった。今後は、人工授精以前の血中MGOを継続的に測定し、受胎性との関連を検討することとした。本年度は、次に、MGOが牛子宮内膜細胞に及ぼす影響を検討した。牛子宮内膜上皮細胞および子宮内膜間質細胞の培養液中にMGO(0.1 mMまたは1 mM)を添加し、6、12または24時間後に細胞を回収して、細胞障害性、細胞増殖率、老化細胞の割合を算出した。子宮内膜上皮細胞ではMGO添加による変化がみられなかった。一方、子宮内膜間質細胞においては、MGO添加後6、12および24時間後の各処置区で、MGO 1 mMを添加した場合の傷害性が対照区と比較して有意に増加し、増殖率は対照区と比較して有意に減少した。また、MGO添加後12時間の処置区で、MGO 1 mMを添加した場合の細胞老化率が対照区と比較して有意に上昇したことから、MGOが牛子宮内膜間質細胞の細胞老化を誘導することが示唆された。さらに、MGOが牛生体の子宮内膜に及ぼす影響を検討するため、穀物含量の高い飼料を給与されていない肉用牛を用いて、発情行動を確認した翌日にMGO溶液(5 mM)を各子宮角内に25 mLずつ投与した。MGOを投与した6日後にバイオプシー鉗子を用いて子宮内膜組織を採取し、DNAマイクロアレイ解析を行った。その結果、対照群と比較してMGO投与群で高発現となった16遺伝子、低発現となったのは6遺伝子であった。
    令和2年度は、当初予定されていなかった子宮内へのMGO単回投与が子宮内膜に及ぼす影響を検討する実験を行ったため、MGOの静脈中への長期投与が子宮内膜に及ぼす影響を検討する実験が未実施となっているものの、乳牛の受胎成績と血中MGO濃度の関連を検討する実験やMGOが子宮内膜細胞の生存性、増殖性、細胞老化に及ぼす影響を検討する実験については、当初の予定どおり実施できており、おおむね順調に研究を進めている。
    令和3年度は、MGOの静脈中への長期投与が子宮内膜に及ぼす影響について検討する実験を進めるとともに、MGOが卵管細胞や胚発生に及ぼす影響を検討する。また、MGOが子宮内膜細胞の細胞老化を誘導する機序についても検討を行う予定である。

  6. In vitro construction of bovine endometrium including uterine glands and the study on the machanisms of bovine conceptus development

    Grant number:19H03105  2019.4 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s) 

  7. ATP-プリン受容体シグナリングによる哺乳類の排卵中枢制御メカニズムの解明

    Grant number:19H03103  2019.4 - 2023.3

    科学研究費助成事業  基盤研究(B)

    井上 直子, 平林 真澄, 松山 秀一, 上野山 賀久, 中村 翔

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    Authorship:Coinvestigator(s) 

    本研究の目的は、ほ乳類の雌の排卵を制御する視床下部視索前野/前腹側室周囲核キスペプチンニューロンに発現するプリン受容体に着目し、ATP-プリン受容体シグナリングが排卵中枢であるキスペプチンニューロンを活性化し排卵に至る神経メカニズムを明らかにすることである。一般的に細胞内のエネルギー通貨として知られるATPは、脳内でプリン作動性神経リガンドとして作用するが生殖機能における役割は不明であった。本研究では、ATP-プリン受容体による排卵中枢キスペプチンニューロンの活性化メカニズムを明らかにするとともに、得られた知見の家畜への応用を検証する。
    本研究は、排卵中枢である視索前野/前腹側室周囲核キスペプチンニューロンを上位から制御する新規な神経シグナルとしてATP-プリン受容体シグナリングに着目し、プリン作動性神経の同定と、同神経の活性化メカニズムの解明により、いつ、どのようなメカニズムによりATPシグナルが前腹側室周囲核キスペプチンニューロンに入力・活性化するのかを明らかにすることで、排卵制御の新たな神経機構を解明することを目的としている。
    本年度は、ラットを用いた組織学的解析をすすめ、排卵の指標である黄体形成ホルモンのサージ状分泌が生じる前にプリン作動性神経細胞が活性化する神経核を複数特定した。また、それらの神経核のプリン作動性神経細胞には、エストロジェン受容体αが発現していることも見いだした。現在、神経トレーサー解析により、これらのプリン作動性神経細胞から前腹側室周囲核キスペプチンニューロン近傍への神経投射を解析中である。
    また本年度は、キスペプチンニューロン(Kiss1遺伝子)特異的にCre組み換え酵素を発現する遺伝子改変ラット(Kiss1-Creラット)の作出にも成功した。今後、小麦胚芽レクチンなどの神経トレーサーを発現するアデノ随伴ウィルスベクターを脳領域的特異的に投与し、前腹側室周囲核キスペプチンニューロンに直接入力する神経経路の解析をすすめる予定である。
    in vitro系による解析については、マウス前腹側室周囲核キスペプチンニューロン由来不死化細胞株を購入し、カルシウムイメージング解析を現在実施している。
    今年度、排卵の指標である黄体形成ホルモンのサージ状分泌が生じる前にプリン作動性神経細胞が活性化する神経核を特定できたことと、Kiss1-Creラットの作出に成功したことから、今後神経トレーサー解析をすすめることにより、前腹側室周囲核キスペプチンニューロンに入力するATPシグナルの同定が可能となる。このように研究は順調に進展している。
    また、マウス前腹側室周囲核キスペプチンニューロン由来不死化細胞株によるカルシウムイメージング解析の立ち上げも順調に進んでおり、今後ATP-プリン受容体シグナリングの解析をすすめる予定である。
    今後は、キスペプチンニューロン(Kiss1遺伝子)特異的にCre組み換え酵素を発現する遺伝子改変ラット(Kiss1-Creラット)に、小麦胚芽レクチンなどの神経トレーサーを発現するアデノ随伴ウィルスベクターを脳領域的特異的に投与し、前腹側室周囲核キスペプチンニューロンに直接入力する神経経路の解析をすすめる予定である。また、細胞内 Ca2+濃度変化をキスペプチン放出の指標として用い、マウス前腹側室周囲核キスペプチンニューロン由来不死化細胞へのATPまたはATP・プリン受容体拮抗薬共添加による細胞内Ca2+濃度変化を解析することで、ATP-プリン受容体シグナリングがキスペプチンニューロンを直接活性化するかどうかを検討する予定である。さらに、ラットで明らかにした知見が家畜に応用できるかを検証するため、シバヤギの視索前野近傍にATPを投与し、黄体形成ホルモン分泌を誘起できるかどうかを検証するとともに、プリン受容体拮抗薬を投与し血中LH分泌を指標として黄体形成ホルモン一過性大量放出をブロックできるかを検証する予定である。

  8. Elucidation of endometrial regeneration mechanism by mesenchymal epithelial conversion of bovine endometrial stromal SP cells

    Grant number:17K08141  2017.4 - 2020.3

    Grant-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research(C)

    Matsuyama Shuichi

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    Authorship:Principal investigator 

    Grant amount:\4810000 ( Direct Cost: \3700000 、 Indirect Cost:\1110000 )

    In this study, the RNA-seq analysis of side population (SP) cell identified in bovine endometrium revealed that SP cells have stem/progenitor cell-like properties, and some of them might be derived from bone marrow. The ratio of SP cells in endometrial cells were low immediately after parturition and then gradually increased, suggesting that SP cells are differentiated and proliferated into endometrial cells after parturition. Besides, the ratio of endometrial SP cells in low fertility cows with a high number of calving was similar to that in normal fertility cows with a low number of calving. From this result, it is possible that the ratio of SP cells is not affected by parity, and the number of SP cells is unlikely to affect fertility in cows.

  9. ウシ子宮内膜再生メカニズムの解明

    Grant number:15K18806  2015.4 - 2017.3

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

  10. Establishment and evaluation of bovine induced trophoblast cell line and contruction of in vitro implantation model using this cell line.

    Grant number:26292168  2014.4 - 2018.3

    Kimura Koji

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    Authorship:Coinvestigator(s) 

    It is necessary for improvement of pregnancy rate in cattle to clarify the mechanisms involved in its embryo development, implantation and establishment of pregnancy. The aims of the present study are to establish the bovine induced pluripotent stem cell and induced trophoblastic cells. Bovine amnion derived cells (bADC) were introduced with transposone expression vectors (piggyBac vectors) containing doxycycline (Dox)-inducible transcription factors. Colonies were selected by morphology criteria and then their characteristics were evaluated by expression of stem cell or trophectoderm markers. We established both of bovine iPS cell lines which can be differentiated to germ cells in vivo and trophoblast cell lines which processed trophoblast stem cell-like characteristics and the potential to differentiate into the extra-embryonic cell lineage.

  11. 子宮内膜再生に寄与するウシ子宮内膜幹細胞の同定

    Grant number:25850225  2013.4 - 2015.3

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

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Industrial property rights 3

  1. 受胎性促進剤

    松山秀一、木村康二

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    Application no:特願2016-207865 

  2. 牛の過排卵誘起用皮下注射剤

    木村康二、松山秀一、石井健容、関誠、濱田俊哉

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    Application no:PCT/JP2014/063672 

  3. 牛の過排卵誘起用皮下注射剤

    木村康二、松山秀一、石井健容、関誠、濱田俊哉

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    Application no:特願2013-151440