Updated on 2022/04/15

写真a

 
SUZUKI Miho
 
Organization
Graduate School of Medicine Center for Neurological Diseases and Cance Division Assistant Professor
Graduate School
Graduate School of Medicine
Undergraduate School
School of Medicine Department of Medicine
Title
Assistant Professor
External link

Degree 1

  1. 博士(理学) ( 2001.3   京都大学 ) 

Research Interests 2

  1. long non-coding RNA

  2. 長鎖非翻訳RNA

Research Areas 2

  1. Life Science / Molecular biology

  2. Life Science / Molecular biology

Current Research Project and SDGs 1

  1. 核内RNAとエピジェネティクス制御

Research History 1

  1. Nagoya University   Graduate School of Medicine Center for Neural Disease and Cancer Division   Assistant Professor

    2018.12

Education 1

  1. Nagoya University

    1992.4 - 1996.3

Professional Memberships 4

  1. THE JAPANASE SOCIETY FOR EPIGENETICS

      More details

  2. THE MOLECULAR BIOLOGY SOCIETY OF JAPAN

      More details

  3. 日本エピジェネティクス研究会

  4. 日本癌学会

Awards 1

  1. 2021 JB審査員賞

    2021.11   日本生化学会  

     More details

    Award type:Honored in official journal of a scientific society, scientific journal 

 

Papers 10

  1. Cancer-Specific Targeting of Taurine-Upregulated Gene 1 Enhances the Effects of Chemotherapy in Pancreatic Cancer.

    Tasaki Y, Suzuki M, Katsushima K, Shinjo K, Iijima K, Murofushi Y, Naiki-Ito A, Hayashi K, Qiu C, Takahashi A, Tanaka Y, Kawaguchi T, Sugawara M, Kataoka T, Naito M, Miyata K, Kataoka K, Noda T, Gao W, Kataoka H, Takahashi S, Kimura K, Kondo Y

    Cancer research     2021.3

  2. Structural dynamics of DNA depending on methylation pattern Invited Reviewed

      Vol. 103 ( 1-1 ) page: 012404   2021.1

  3. Autocrine HGF/c-Met signaling pathway confers aggressiveness in lymph node adult T-cell leukemia/lymphoma.

    Totani H, Shinjo K, Suzuki M, Katsushima K, Mase S, Masaki A, Ito A, Ri M, Kusumoto S, Komatsu H, Ishida T, Inagaki H, Iida S, Kondo Y

    Oncogene   Vol. 39 ( 35 ) page: 5782 - 5794   2020.8

  4. A novel sensitive detection method for DNA methylation in circulating free DNA of pancreatic cancer. Reviewed International journal

    Keiko Shinjo, Kazuo Hara, Genta Nagae, Takayoshi Umeda, Keisuke Katsushima, Miho Suzuki, Yoshiteru Murofushi, Yuta Umezu, Ichiro Takeuchi, Satoru Takahashi, Yusuke Okuno, Keitaro Matsuo, Hidemi Ito, Shoji Tajima, Hiroyuki Aburatani, Kenji Yamao, Yutaka Kondo

    PloS one   Vol. 15 ( 6 ) page: e0233782 - e0233782   2020

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Public Library of Science (PLoS)  

    Despite recent advances in clinical treatment, pancreatic cancer remains a highly lethal malignancy. In order to improve the survival rate of patients with pancreatic cancer, the development of non-invasive diagnostic methods using effective biomarkers is urgently needed. Here, we developed a highly sensitive method to detect DNA methylation in cell-free (cf)DNA samples based on the enrichment of methyl-CpG binding (MBD) protein coupled with a digital PCR method (MBD-ddPCR). Five DNA methylation markers for the diagnosis of pancreatic cancer were identified through DNA methylation microarray analysis in 37 pancreatic cancers. The sensitivity and specificity of the five markers were validated in another independent cohort of pancreatic cancers (100% and 100%, respectively; n = 46) as well as in The Cancer Genome Atlas data set (96% and 90%, respectively; n = 137). MBD-ddPCR analysis revealed that DNA methylation in at least one of the five markers was detected in 23 (49%) samples of cfDNA from 47 patients with pancreatic cancer. Further, a combination of DNA methylation markers and the KRAS mutation status improved the diagnostic capability of this method (sensitivity and specificity, 68% and 86%, respectively). Genome-wide MBD-sequencing analysis in cancer tissues and corresponding cfDNA revealed that more than 80% of methylated regions were overlapping; DNA methylation profiles of cancerous tissues and cfDNA significantly correlated with each other (R = 0.97). Our data indicate that newly developed MBD-ddPCR is a sensitive method to detect cfDNA methylation and that using five marker genes plus KRAS mutations may be useful for the detection of pancreatic cancers.

    DOI: 10.1371/journal.pone.0233782

    PubMed

    researchmap

  5. Pathogenic Epigenetic Consequences of Genetic Alterations in IDH-Wild-Type Diffuse Astrocytic Gliomas. Reviewed International journal

    Fumiharu Ohka, Keiko Shinjo, Shoichi Deguchi, Yusuke Matsui, Yusuke Okuno, Keisuke Katsushima, Miho Suzuki, Akira Kato, Noboru Ogiso, Akane Yamamichi, Kosuke Aoki, Hiromichi Suzuki, Shinya Sato, Nirmala Arul Rayan, Shyam Prabhakar, Jonathan Göke, Teppei Shimamura, Reo Maruyama, Satoru Takahashi, Akio Suzumura, Hiroshi Kimura, Toshihiko Wakabayashi, Hui Zong, Atsushi Natsume, Yutaka Kondo

    Cancer research   Vol. 79 ( 19 ) page: 4814 - 4827   2019.10

     More details

    Language:English  

    Gliomas are classified by combining histopathologic and molecular features, including isocitrate dehydrogenase (IDH) status. Although IDH-wild-type diffuse astrocytic glioma (DAG) shows a more aggressive phenotype than IDH-mutant type, lack of knowledge regarding relevant molecular drivers for this type of tumor has hindered the development of therapeutic agents. Here, we examined human IDH-wild-type DAGs and a glioma mouse model with a mosaic analysis with double markers (MADM) system, which concurrently lacks p53 and NF1 and spontaneously develops tumors highly comparable with human IDH-wild-type DAG without characteristic molecular features of glioblastoma (DAG-nonMF). During tumor formation, enhancer of zeste homolog (EZH2) and the other polycomb repressive complex 2 (PRC2) components were upregulated even at an early stage of tumorigenesis, together with an increased number of genes with H3K27me3 or H3K27me3 and H3K4me3 bivalent modifications. Among the epigenetically dysregulated genes, frizzled-8 (Fzd8), which is known to be a cancer- and stem cell reprogramming-related gene, was gradually silenced during tumorigenesis. Genetic and pharmacologic inhibition of EZH2 in MADM mice showed reactivation of aberrant H3K27me3 target genes, including Fzd8, together with significant reduction of tumor size. Our study clarifies a pathogenic molecular pathway of IDH-wild-type DAG-nonMF that depends on EZH2 activity and provides a strong rationale for targeting EZH2 as a promising therapeutic approach for this type of glioma. SIGNIFICANCE: EZH2 is involved in the generation of IDH-wild-type diffuse astrocytic gliomas and is a potential therapeutic target for this type of glioma. GRAPHICAL ABSTRACT: http://cancerres.aacrjournals.org/content/canres/79/19/4814/F1.large.jpg.

    DOI: 10.1158/0008-5472.CAN-19-1272

    PubMed

    researchmap

  6. The Ciona intestinalis cleavage clock is independent of DNA methylation Reviewed

    Miho M. Suzuki, Tomoko Mori, Noriyuki Satoh

    GENOMICS   Vol. 108 ( 3-4 ) page: 168 - 176   2016.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ACADEMIC PRESS INC ELSEVIER SCIENCE  

    The initiation of embryonic gene expression in ascidian embryos appears to be tightly regulated by the number of DNA replication cycles. DNA methylation is thought to contribute to the clock mechanism that counts the rounds of DNA replication. We used mass spectrometry and whole genome bisulfite sequencing to characterize DNA methylation changes that occur in early developmental stages of the ascidian, Ciona intestinalis. We found that global DNA methylation in early Ciona development was static, and a base-wise comparison between the genomes of consecutive developmental stages found no DNA demethylation that was related to zygotic gene activation. Additionally, 5hmC was hardly detected by mass spectrometry in the developmental samples, suggesting a lack of demethylation mediated by ten eleven translocation (TET) methylcytosine dioxygenase in C. intestinalis. We conclude that DNA methylation is not involved in regulating DNA replication-dependent transcriptional activation. (C) 2016 Elsevier Inc. All rights reserved.

    DOI: 10.1016/j.ygeno.2016.10.001

    Web of Science

    researchmap

  7. Identical sets of methylated and nonmethylated genes in Ciona intestinalis sperm and muscle cells.

    Suzuki MM, Yoshinari A, Obara M, Takuno S, Shigenobu S, Sasakura Y, Kerr AR, Webb S, Bird A, Nakayama A

    Epigenetics & chromatin   Vol. 6 ( 1 ) page: 38   2013.11

     More details

  8. Maternal factor-mediated epigenetic gene silencing in the ascidian Ciona intestinalis.

    Sasakura Y, Suzuki MM, Hozumi A, Inaba K, Satoh N

    Molecular genetics and genomics : MGG   Vol. 283 ( 1 ) page: 99 - 110   2010.1

  9. CpG methylation is targeted to transcription units in an invertebrate genome.

    Suzuki MM, Kerr AR, De Sousa D, Bird A

    Genome research   Vol. 17 ( 5 ) page: 625 - 31   2007.5

     More details

  10. Transposable element in fish

    Koga A, Suzuki M, Inagaki H, Bessho Y, Hori H

    NATURE   Vol. 383 ( 6595 ) page: 30 - 30   1996.9

     More details

▼display all

Books 2

  1. 動物の事典

    末光 隆志( Role: Joint author ,  第3章 動物遺伝子の発現)

    朝倉書店  2020.11  ( ISBN:4254171668

     More details

    Language:Japanese Book type:Dictionary, encyclopedia

  2. 遺伝子発現制御機構

    田村隆明 浦聖恵( Role: Joint author ,  第1部 クロマチンの構造とその変換)

    東京化学同人  2017.3  ( ISBN:9784807909179

     More details

    Language:Japanese Book type:Textbook, survey, introduction

MISC 3

  1. ヒストンメチル化制御阻害剤

    鈴木美穂 近藤豊

    遺伝子医学MOOK 36号「エピゲノムで新たな解明 が進む『先天性疾患』」     2021.4

     More details

    Authorship:Lead author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  2. Operating principle of EZH2 inhibitor and current development situation

      Vol. 266 ( 11 ) page: 834 - 839   2018.9

     More details

    Language:Japanese  

    CiNii Books

    researchmap

  3. 長鎖非コード RNA をターゲットにした膠芽腫の治療.

    鈴木美穂, 勝島啓佑, 近藤豊

    Medical Science Digest   Vol. 44   page: 238 - 40   2018.5

     More details

    Authorship:Lead author   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    researchmap

Presentations 4

  1. 長鎖非翻訳RNA TUG1は核スペックル近傍で複製フォークを保護する Invited

    鈴木美穂

    第14回日本エピジェネティクス研究会年会  2021.3.31 

     More details

    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:オンライン  

  2. Spatiotemporal regulation of long non-coding RNA to propagate Cancer Cells Invited

    2021.3.30 

     More details

    Event date: 2021.3

    Presentation type:Symposium, workshop panel (nominated)  

  3. Spatiotemporal regulation of replication stress by TUG1 as a prerequisite for cancer cell proliferation Invited

    Miho Suzuki

    2020.12.3 

     More details

    Event date: 2020.12

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

  4. 長鎖非翻訳RNA TUG1による時空間的DNA複製ストレス制御 Invited

    鈴木美穂

    日本環境変異原学会第49回大会  2020.11.26 

     More details

    Event date: 2020.11

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:沼津  

Research Project for Joint Research, Competitive Funding, etc. 1

  1. DDS技術を基盤とした革新的がん治療法の開発

    Grant number:21cm0106202h0006  2016.4 - 2022.3

    次世代がん医療創生研究事業 

    鈴木美穂

      More details

    Authorship:Coinvestigator(s)  Grant type:Competitive

KAKENHI (Grants-in-Aid for Scientific Research) 6

  1. がん細胞の複製ストレスを解消する新規lncRNAの機能解明

    Grant number:20K07570  2020.4 - 2023.3

    日本学術振興会  科学研究費助成事業  基盤研究(C)

    鈴木 美穂

      More details

    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

    がん細胞に特徴的な高い複製ストレスは、ゲノム不安定性をもたらし、発がんの促進やがんの進展を加速させることがよく知られている。しかし、がん細胞が高い複製ストレスに晒されながらも、複製を完了させ細胞増殖していく機構は完全に解明されていない。本研究では、①がん細胞の複製ストレスの解消に関わる新規lncRNAの同定 ②lncRNAと相互作用するタンパク質の同定から、未知のがん細胞複製ストレス解消メカニズムを明らかにする。

  2. the mechanism that eliminates transcription noise and maintains cell uniformity

    Grant number:17K07247  2017.4 - 2020.3

    Suzuki Miho

      More details

    Authorship:Principal investigator 

    Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )

    To elucidate the mechanism that regulates the uniformity and variation of gene expression levels among individual cells, we focused on RNA molecules retained in the nucleus and analyzed them. We found that RNAs that require more time for pre-mRNA splicing are more likely to stay in the nucleus. In addition, we found that lncRNAs stocked in the nucleus, specifically in cancer cells, are involved in the regulation of DNA replication. This study shows that nuclear RNAs play an important role in the maintenance of cellular homeostasis.

  3. 遺伝子転写領域のクロマチン構造とエピジェネティック制御の解明

    Grant number:14J40145  2014.4 - 2017.3

      More details

    Authorship:Other 

    平成27年度に、カタユウレイボヤのDNAメチル化修飾をゲノムワイドに調べた。3つの発生ステージを比較した。平成28年度は、このデータに関して種々の解析を行い、論文として発表した(Genomics 2016)。主な結果は、カタユウレイボヤのDNAメチル化修飾は発生過程を通じて常に安定であること、遺伝子の発現変化とは無関係であること、また脱メチル化に関与する5hmシトシン修飾は検出されなかったこと、である。
    また、このデータから新たな発見が得られた。カタユウレイボヤ集団内にはDNAメチル化多型が存在する。DNAメチル化多型は、全ゲノム中約20アレルに見出された。これらのアレルのDNAメチル化状態は各個体によって異なる。また、DNAメチル化多型は環境ではなく、遺伝的に決定されていることが示唆された。今後は、DNAメチル化状態を決定する遺伝情報、ゲノム配列を同定する。
    28年度が最終年度であるため、記入しない。
    28年度が最終年度であるため、記入しない。

  4. Phylogenetic analysis and rapid identification of spider mites based on RNA-sequencing data

    Grant number:25292033  2013.4 - 2017.3

    Gotoh Tetsuo

      More details

    Authorship:Coinvestigator(s) 

    Molecular information of spider mites is increasingly used to answer taxonomic and systematic questions. The COI gene and ITS2 region of nuclear ribosomal RNA have been used for phylogenetic reconstruction within the Tetranychidae, but these sequences have not consistently resolved genus-level phylogenetic relationships, because of low bootstrap values. So, we performed phylogenomics analysis for spider mites, derived from comparative RNA-Seq data for 88 strains of 87 species belonging to 15 genera. A phylogenetic tree using 652 orthologous protein-coding genes shared among taxa, was well-resolved and strongly supported for almost all nodes (67/70), allowing us to consider the phylogenetic relationships among genera of Tetranychidae. The topology presented here does not fully agree with the current taxonomic treatment, because the four genera were polyphyletic. These results indicate that the diagnostic morphological characters of the genera of Tetranychidae must be reconsidered.

  5. Methylated DNA binding protein MBD2/3 and its interacting proteins in Ciona intestinalis

    Grant number:21810039  2009 - 2010

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity start-up  Grant-in-Aid for Research Activity start-up

    SUZUKI Miho

      More details

    Authorship:Principal investigator 

    Grant amount:\2756000 ( Direct Cost: \2120000 、 Indirect Cost:\636000 )

    MBD2/3is highly expressed in early developmental stages of Ciona intestinalis. A knock down experiment depleting MBD2/3 protein in embryos caused severe developmental defects. The result indicated an important role of DNA methylation and methylated DNA binding protein in regulation of gene expression. Genome-wide distribution of MBD2/3 was limited to methylated gene body region. MBD2/3 possibly regulates expression of those more than 100gene targets.

    researchmap

  6. メダカ・トランスポゾンTo12による脊椎動物遺伝子改変系の構築

    Grant number:98J03521  1998 - 2000

    日本学術振興会  科学研究費助成事業 特別研究員奨励費  特別研究員奨励費

    鈴木 美穂

      More details

▼display all

 

Teaching Experience (On-campus) 3

  1. 遺伝と遺伝子

    2020

  2. 基礎セミナーA

    2020

  3. 病理病体学

    2020