Updated on 2024/04/02

写真a

 
OBARA Keisuke
 
Organization
Graduate School of Science Associate professor
Graduate School
Graduate School of Science
Undergraduate School
School of Science Department of Biological Science
Title
Associate professor

Degree 1

  1. 博士(理学) ( 2004.3   東京大学 ) 

Research Interests 3

  1. Biomembrane

  2. lipid

  3. Proteolysis

Research Areas 3

  1. Life Science / Molecular biology

  2. Life Science / Cell biology

  3. Life Science / Functional biochemistry

Current Research Project and SDGs 1

  1. Homeostasis of biomembrane

Research History 5

  1. Nagoya University   Associate professor

    2024.4

  2. Nagoya University   Lecturer

    2022.7 - 2024.3

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    Country:Japan

  3. Nagoya University   Assistant Professor

    2017.7 - 2022.7

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    Country:Japan

  4. Hokkaido University   Faculty of Pharmaceutical Sciences   Assistant Professor

    2008.5 - 2017.6

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    Country:Japan

  5. National Institute for Basic Biology   Postdoctoral fellow

    2004.4 - 2008.5

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    Country:Japan

Education 2

  1. The University of Tokyo   Graduate School, Division of Science

    1999.4 - 2004.3

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    Country: Japan

  2. The University of Tokyo   Faculty of Science

    1995.4 - 1999.3

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    Country: Japan

Professional Memberships 4

  1. Yeast Genetics Society of Japan

  2. Japan Society for Cell Biology

  3. The Japanease Biochemical Society

  4. The Molecular Biology Society of Japan

Awards 1

  1. 日本細胞生物学会若手最優秀発表賞

    2013.6   日本細胞生物学会  

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

 

Papers 53

  1. Proteolysis of adaptor protein Mmr1 during budding is necessary for mitochondrial homeostasis in <i>Saccharomyces cerevisiae</i> Reviewed

    Obara, K; Yoshikawa, T; Yamaguchi, R; Kuwata, K; Nakatsukasa, K; Nishimura, K; Kamura, T

    NATURE COMMUNICATIONS   Vol. 13 ( 1 )   2022.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41467-022-29704-8

    Web of Science

    PubMed

  2. E3 ligases regulate organelle inheritance in yeast. Invited Reviewed

    Obara K, Nishimura K, and Kamura T

    Cells   Vol. 13   2024.2

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.3390/cells13040292

  3. Targeted protein degradation systems: Controlling protein stability using E3 ubiquitin ligases in eukaryotic species. Invited Reviewed

    Ogawa Y, Ueda TP, Obara K, Nishimura K, and Kamura T

    Cells   Vol. 13   2024.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.3390/cells13020175

  4. Development of AlissAID system targeting GFP or mCherry fusion protein. Reviewed

    Ogawa Y, Nishimura K, Obara K, Kamura T

    PLoS Genetics   Vol. 19 ( 6 )   2023.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1371/journal.pgen.1010731

  5. Regulator of Awn Elongation 3, an E3 ubiquitin ligase, is responsible for loss of awns during African rice domestication Reviewed International coauthorship

    1) Bessho-Uehara K, Masuda K, Wang DR, Angeles-Shim RB, Obara K, Nagai K, Murase R, Aoki S, Furuta T, Miura K, Wu J, Yamagata Y, Yasui H, Kantar MB, Yoshimura A, Kamura T, McCouch SR, and Ashikari M

    Proc Natl Acad Sci USA   Vol. 120   2023.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1073/pnas.2207105120

  6. Development of AlissAID system targeting GFP or mCherry fusion protein

    Ogawa Y, Nishimura K, Obara K, Kamura T

    bioRxive     2022.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1101/2022.12.12.520189

  7. Breaking the clip for cargo unloading from motor proteins ~its mechanism and significance~ Invited Reviewed International journal

    Obara K and Kamura T

    Microbial Cell   Vol. 9 ( 6 ) page: 133 - 135   2022.5

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.15698/mic2022.06.779

  8. Dot6/Tod6 degradation fine-tunes the repression of ribosome biogenesis under nutrient-limited conditions Reviewed

    Kusama K, Suzuki Y, Kurita E, Kawarasaki T, Obara K, Okumura F, Kamura T, and Nakatsukasa K

    iScience   Vol. 25 ( 3 )   2022.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1016/j.isci.2022.103986

  9. The Rim101 pathway mediates adaptation to external alkalization and altered lipid asymmetry: hypothesis describing the detection of distinct stresses by the Rim21 sensor protein

    Obara, K; Kamura, T

    CURRENT GENETICS   Vol. 67 ( 2 ) page: 213 - 218   2021.4

  10. 細胞膜脂質非対称の感知と細胞応答

    小原 圭介

    アグリバイオ   Vol. 57   page: 48 - 50   2021.1

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    Authorship:Lead author, Last author, Corresponding author   Language:Japanese   Publishing type:Research paper (scientific journal)  

  11. Rapid turnover of transcription factor Rim101 confirms a flexible adaptation mechanism against environmental stress in<i>Saccharomyces cerevisiae</i> Reviewed

    Obara, K; Higuchi, M; Ogura, Y; Nishimura, K; Kamura, T

    GENES TO CELLS   Vol. 25 ( 10 ) page: 651 - 662   2020.10

  12. <i>N</i>-glycosylation of Rim21 at an Unconventional Site Fine-tunes Its Behavior in the Plasma Membrane Reviewed

    Obara, K; Kotani, T; Nakatogawa, H; Kihara, A; Kamura, T

    CELL STRUCTURE AND FUNCTION   Vol. 45 ( 1 ) page: 1 - 8   2020

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1247/csf.19021

    Web of Science

    PubMed

  13. Sphingolipids activate the endoplasmic reticulum stress surveillance pathway

    Piña, F; Yagisawa, F; Obara, K; Gregerson, JD; Kihara, A; Niwa, M

    JOURNAL OF CELL BIOLOGY   Vol. 217 ( 2 ) page: 495 - 505   2018.2

  14. Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2

    Okumura, F; Joo-Okumura, A; Obara, K; Petersen, A; Nishikimi, A; Fukui, Y; Nakatsukasa, K; Kamura, T

    MOLECULAR BIOLOGY OF THE CELL   Vol. 28 ( 24 ) page: 3532 - 3541   2017.11

  15. Systematic analysis of Ca<SUP>2+</SUP> homeostasis in Saccharomyces cerevisiae based on chemical-genetic interaction profiles

    Ghanegolmohammadi, F; Yoshida, M; Ohnuki, S; Sukegawa, Y; Okada, H; Obara, K; Kihara, A; Suzuki, K; Kojima, T; Yachie, N; Hirata, D; Ohya, Y

    MOLECULAR BIOLOGY OF THE CELL   Vol. 28 ( 23 ) page: 3415 - 3427   2017.11

  16. Loop 5 region is important for the activity of the long-chain base transporter Rsb1

    Makuta, H; Obara, K; Kihara, A

    JOURNAL OF BIOCHEMISTRY   Vol. 161 ( 2 ) page: 207 - 213   2017.2

  17. The Rim101 pathway contributes to ER stress adaptation through sensing the state of plasma membrane

    Obara, K; Kihara, A

    BIOCHEMICAL JOURNAL   Vol. 474   page: 51 - 63   2017.1

  18. Loop 5 region is important for the activity of the long-chain base transporter Rsb1. Reviewed

    Makuta H, Obara K, and Kihara A

    J Biochem   Vol. 161   page: 207-213   2017

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    Language:English   Publishing type:Research paper (scientific journal)  

  19. Ubiquitin ligase SPSB4 diminishes cell repulsive responses mediated by EphB2. Reviewed

    Okumura F, Joo-Okumura A, Obara K, Petersen A, Nishikimi A, Fukui Y, Nakatsukasa K, Kamura T

    Mol Biol Cell   Vol. 28   page: 3532-3541   2017

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    Language:English   Publishing type:Research paper (scientific journal)  

  20. Systematic analysis of Ca2+ homoeostasis in Saccharomyces cerevisiae based on chemical-genetic interaction profiles. Reviewed

    Ghanegolmohammadi F, Yoshida M, Ohnuki S, Sukegawa Y, Okada H, Obara K, Kihara A, Suzuki K, Kojima T, Yachie N, Hirata D, and Ohya Y

    Mol Biol Cell   Vol. 28   page: 3415-3427   2017

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    Language:English   Publishing type:Research paper (scientific journal)  

  21. The Rim101 pathway contributes to ER stress adaptation through sensing the state of plasma membrane. Reviewed

    Obara K and Kihara A

    Biochem J   Vol. 474   page: 51-63   2017

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    Language:English   Publishing type:Research paper (scientific journal)  

  22. 医学・農学的応用を見据えた細胞膜脂質非対称センサーの分子解剖及びエンジニアリング Reviewed

    小原圭介

      Vol. 30   page: 137-138   2016

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    Language:Japanese   Publishing type:Research paper (bulletin of university, research institution)  

  23. Opt2 mediates the exposure of phospholipids during cellular adaptation to altered lipid asymmetry. Reviewed

    Yamauchi S, Obara K, Uchibori K, Kamimura A, Azumi K, and Kihara A

    J Cell Sci   Vol. 128   page: 61-69   2015

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    Language:English   Publishing type:Research paper (scientific journal)  

  24. Mechanism for sensing lipid asymmetry of the plasma membrane and external alkalization. Reviewed

    Obara K, Nishino K and Kihara A

    Yeast   Vol. 32   page: 195   2015

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    Language:English  

  25. The C-terminal cytosolic region of Rim21 senses alterations in plasma membrane lipid composition: Insights into sensing mechanisms for plasma membrane lipid asymmetry. Reviewed

    Nishino K, Obara K, and Kihara A

    J Biol Chem   Vol. 290   page: 30797-30805   2015

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    Language:English   Publishing type:Research paper (scientific journal)  

  26. Rim21 senses alteration in plasma membrane lipid asymmetry and elicits the signal at the plasma membrane in a ubiquitination-dependent manner. Reviewed

    Obara K, Nishino K and Kihara A

    FEBS J   Vol. 281   page: 207   2014

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    Language:English   Publishing type:Research paper (scientific journal)  

  27. Signaling events of the Rim101 pathway occur at the plasma membrane in a ubiquitination-dependent manner. Reviewed

    Obara K and Kihara A

    Mol Cell Biol   Vol. 34   page: 3525-3534   2014

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    Language:English   Publishing type:Research paper (scientific journal)  

  28. Yeast and mammalian autophagosomes exhibit distinct phosphatidylinositol 3-phosphate asymmetries. Reviewed

    Cheng J, Fujita A, Yamamoto H, Tatematsu T, Kakuta S, Obara K, Ohsumi Y, and Fujimoto T

    Nat Commun   Vol. 5   2014

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi: 10.1038/ncomms4207.

  29. Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids Reviewed

    Obara Keisuke, Kojima Ryo, Kihara Akio

    JOURNAL OF LIPID RESEARCH   Vol. 54 ( 3 ) page: 831 - 842   2013.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1194/jlr.M034678

    Web of Science

  30. Effects on vesicular transport pathways at the late endosome in cells with limited very long-chain fatty acids. Reviewed

    Obara K, Kojima R and Kihara A

    J Lipid Res   Vol. 54   page: 831-842   2013

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    Language:English   Publishing type:Research paper (scientific journal)  

  31. オートファジーに関わるタンパク質とその役割 Reviewed

    小林孝史、小原圭介、大隅良典

    ファルマシア   Vol. 49   page: 508-512   2013

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    Language:Japanese  

  32. Study on the function of sphingolipids during the starvation response.

    Obara K

    Report of the Noda Institute for Scientific research   Vol. 57   page: 48-50   2013

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    Language:English  

  33. Unperverted synthesis of complex sphingolipids is essential for cell survival under nitrogen starvation. Reviewed

    Yamagata M, Obara K and Kihara A

    Genes Cells   Vol. 18   page: 650-659   2013

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    Language:English   Publishing type:Research paper (scientific journal)  

  34. オートファゴソーム形成におけるホスファチジルイノシトール3−キナーゼの役割 Invited

    小原圭介

    生体の科学   Vol. 63   page: 486-487   2012

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    Language:Japanese  

  35. Membrane protein Rim21 plays a central role in sensing ambient pH in Saccharomyces cerevisiae. Reviewed

    Obara K, Yamamoto H, and Kihara A

    J Biol Chem   Vol. 287   page: 38473-38481   2012

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    Language:English   Publishing type:Research paper (scientific journal)  

  36. Guidelines for the use and interpretation of assays for monitoring autophagy. Reviewed

    Klionsky DJ et al.

    Autophagy   Vol. 8   page: 445-544   2012

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  37. Sphingolipid synthesis is involved in autophagy in Saccharomyces cerevisiae. Reviewed

    Yamagata M, Obara K and Kihara A

    Biochem Biophys Res Commun   Vol. 410   page: 786-791   2011

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    Language:English   Publishing type:Research paper (scientific journal)  

  38. Involvement of sphingolipids in autophagy in Saccharomyces cerevisiae. Reviewed

    Yamagata M, Obara K and Kihara A

    Chem Phys Lipids   Vol. 164   page: 34-35   2011

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    Language:English  

  39. Atg14, a key player in orchestrating autophagy. Reviewed

    Obara K and Ohsumi Y

    Int J Cell Biol     2011

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    Language:English  

    DOI: doi: 10.1155/2011/713435

  40. PtdIns 3-kinase orchestrates autophagosome formation in yeast. Reviewed

    Obara K and Ohsumi Y

    J Lipids     2011

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: doi: 10.1155/2011/498768

  41. Dynamics and function of PtdIns(3)P in autophagy. Reviewed

    Obara K and Ohsumi Y

    Autophagy   Vol. 4   page: 952-954   2008

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  42. The Atg18-Atg2 complex is recruited to autophagic membranes via PtdIns(3)P and exerts an essential function. Reviewed

    Obara K, Sekito T, Niimi K and Ohsumi Y

    J Biol Chem   Vol. 283   page: 23972-23980   2008

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  43. Transport of phosphatidylinositol 3-phosphate into the vacuole via autophagic membranes in S. cerevisiae. Reviewed

    Obara K, Noda T, Niimi K and Ohsumi Y

    Genes Cells   Vol. 13   page: 537-547   2008

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  44. PI3K signaling of autophagy is required for starvation tolerance and virulence of Cryptococcus neoformans. Reviewed

    Hu G, Hacham M, Waterman SR, Panepinto J, Shin S, Liu X, Gibbons J, Valyi-Nagy T, Obara K, Jaffe HA, Ohsumi Y and Williamson PR

    J Clin Invest   Vol. 118   page: 1186-1197   2008

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  45. Structure of Atg5-Atg16, a complex essential for autophagy. Reviewed

    Matsushita M, Suzuki NN, Obara K, Fujioka Y, Ohsumi Y and Inagaki F

    J Biol Chem   Vol. 282   page: 6763-6772   2007

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  46. Assortment of phosphatidylinositol 3-kinase complexes - Atg14p directs association of complex I to the pre-autophagosomal structure in S. cerevisiae -. Reviewed

    Obara K, Sekito T and Ohsumi Y

    Mol Biol Cell   Vol. 17   page: 1527-1539   2006

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  47. Characterization of yeast Atg proteins.

    Nakatogawa H, Hanada T, Kamada Y, Obara K and Sekito T

      Vol. 51   page: 1457-1463   2006

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    Language:Japanese  

  48. Programmed cell death in xylem differentiation. Invited

    Obara K and Fukuda H

    Programmed cell death in plants (J. Gray eds.)     page: 131-154   2003

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  49. Plant (Zinnia elegans) vessel elements transdifferentiated from mesophyll cells in vitro. Reviewed

    Obara K

    Nat Rev Mol Cell Biol   Vol. 4   page: 10-11   2003

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  50. Plant vessel elements trans-differentiated from mesophyll cells in vitro. Reviewed

    Obara K

    Nat Cell Biol   Vol. 5   page: 15   2003

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  51. The vacuole is a key player of developmentally programmed cell death in plants. Reviewed

    Fukuda H, Yamamoto R, Obara K, Kuriyama H, Ito J

    Eur J Cell Biol   Vol. 82   page: 55-56   2003

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    Language:English  

  52. The use of multiple transcription starts causes the dual targeting of Arabidopsis putative monodehydroascorbate reductase to both mitochondria and chloroplasts. Reviewed

    Obara K, Sumi K and Fukuda H

    Plant Cell Physiol   Vol. 43   page: 697-705   2002

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  53. Direct evidence of active and rapid nuclear degradation triggered by vacuole rupture during programmed cell death in Zinnia. Reviewed

    Obara K, Kuriyama H and Fukuda H

    Plant Physiol   Vol. 125   page: 615-626   2001

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▼display all

Presentations 41

  1. ミトコンドリアの正常な遺伝と機能に欠かせないアダプタータンパク質Mmr1の選択的分解

    小原圭介、吉川拓、山口竜、中務邦夫、西村浩平、嘉村巧

    第45回日本分子生物学会年会  2022.11.30  日本分子生物学会

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    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:幕張  

  2. 細胞膜脂質非対称と細胞外物理化学変数の関係

    小原圭介、安田有那、嘉村巧

    酵母遺伝学フォーラム第55回研究報告会  2022.9.8  酵母遺伝学フォーラム

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:沖縄  

  3. 小分子抗体を利用した標的タンパク質分解系「AlissAID」システムの開発

    小川佳孝、西村浩平、小原圭介、嘉村巧

    第46回日本分子生物学会年会  2023.12 

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    Event date: 2023.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:兵庫県神戸市   Country:Japan  

  4. 翻訳後修飾および液-液相分離を介した新規細胞壁合成調節機構の発見

    加藤黎、小原圭介、小倉佑季、小林恵理花、嘉村巧

    第46回日本分子生物学会年会  2023.12 

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    Event date: 2023.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:兵庫県神戸市   Country:Japan  

  5. Snf1/AMPK依存性糖シグナル経路におけるHxk2のリン酸化機構

    野中一輝、深瀬文奈子、小原圭介、西村浩平、嘉村巧

    第46回日本分子生物学会年会  2023.12 

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    Event date: 2023.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:兵庫県神戸市   Country:Japan  

  6. 最後の機能未知必須タンパク質Pbr1による膜タンパク質の折り畳み補助

    小原圭介

    第16回小胞体ストレス研究会  2023.9.30 

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:金沢   Country:Japan  

  7. 遺伝子工学を用いた脂肪滴形成機構の解明と油脂の増産

    小原圭介

    第13回リピッド合同コンファレンス  2023.9.28 

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:御殿場   Country:Japan  

  8. 出芽酵母を用いた油脂生産の試み

    小原圭介、飯嶋大佑、Joya EL Khatib、嘉村巧

    酵母遺伝学フォーラム第56回研究報告会  2023.8.31 

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    Event date: 2023.8 - 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  9. 分解及び局在に着目した細胞壁合成関連因子Dse3の動態制御機構の解析

    加藤黎、小原圭介、嘉村巧

    酵母遺伝学フォーラム第56回研究報告会  2023.8.31 

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    Event date: 2023.8 - 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:新潟大学:新潟県新潟市   Country:Japan  

  10. Some applications of auxin dependent degradation system in eukaryotic species. International conference

    Ogawa Y, Nishimura K, Obara K, Kamura T

    Chromatin Architecture: Structure and Function  2023.1.24 

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    Event date: 2023.1

    Language:English   Presentation type:Oral presentation (general)  

    Venue:online  

  11. 真核生物種におけるオーキシン依存的なタンパク質分解系の利用法

    西村浩平、小川佳孝、福原佳乃、小原圭介、嘉村巧

    第45回日本分子生物学会年会  2022.11.30  日本分子生物学会

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    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Poster presentation  

  12. 迅速かつ低毒性に標的タンパク質を分解するTPB-ssAIDシステムの開発

    小川佳孝、小原圭介、西村浩平、嘉村巧

    第45回日本分子生物学会年会  2022.12.2  日本分子生物学会

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    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Poster presentation  

  13. HECT型E3リガーゼMtn1によるリボソーム生合成の調節機構の解析

    永本愛奈、酒井洋二、西村浩平、小原圭介、嘉村巧

    第45回日本分子生物学会年会  2022.12.1  日本分子生物学会

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    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Poster presentation  

  14. 出芽酵母短寿命タンパク質Nih1によるグルコース飢餓応答遺伝子の発現調節

    野中一輝、小原圭介、西村浩平、嘉村巧

    第45回日本分子生物学会年会  2022.12.1  日本分子生物学会

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    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Poster presentation  

  15. ヒト培養細胞におけるピルビン酸キナーゼの活性制御機構の解明

    福原佳乃、西村浩平、小原圭介、嘉村巧

    第45回日本分子生物学会年会  2022.12.1  日本分子生物学会

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    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Poster presentation  

  16. 最後の機能未知必須タンパク質による新生膜タンパク質の折り畳み補助

    小原圭介

    第15回小胞体ストレス研究会  2022.7.30  小胞体ストレス研究会

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    Event date: 2022.7

    Language:Japanese   Presentation type:Poster presentation  

    Venue:京都  

  17. アダプタータンパク質Mmr1の選択的分解を介したミトコンドリア遺伝の制御

    小原圭介、吉川拓、嘉村巧

    酵母遺伝学フォーラム第54回研究報告会  2021.9.1  酵母遺伝学フォーラム

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    Event date: 2021.8 - 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:Web開催   Country:Japan  

  18. アダプタータンパク質Mmr1の分解を介したミトコンドリア遺伝の制御

    小原圭介、吉川拓、西村浩平、嘉村巧

    第73回日本細胞生物学会大会  2021.7.1  日本細胞生物学会

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    Event date: 2021.6 - 2021.7

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:Web開催   Country:Japan  

  19. 転写因子Rim101の迅速な分解からみたストレス応答経路同士の相互作用

    小原圭介、樋口舞、嘉村巧

    酵母遺伝学フォーラム第53回研究報告会  2020.9 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:Covid-19禍によりWeb開催   Country:Japan  

  20. 脂質非対称性の感知機構から見えてきた細胞膜の新たな役割 Invited

    小原 圭介

    第72回日本生物工学会大会  2020.9.4 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:Covid-19禍によりWeb開催   Country:Japan  

  21. 細胞膜脂質非対称バイオセンサーの開発

    小原圭介、安田有那、嘉村巧

    第19回日本蛋白質学会年会・第71回日本細胞生物学会大会 合同年次大会  2019.6 

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    Event date: 2019.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:兵庫県神戸市   Country:Japan  

  22. 細胞膜脂質非対称センサーが細胞内外のストレスを感知する仕組み Invited

    小原圭介

    日本遺伝学会第89回大会  2017.9 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:岡山県岡山市   Country:Japan  

  23. Sensing mechanism of alterations in plasma membrane lipid asymmetry and external alkalization. International conference

    Obara K, Nishino K and Kihara A

    14th International Congress on Yeasts (ICY14)  2016.11 

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    Event date: 2016.11

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Venue:Awaji, Hyogo   Country:Japan  

  24. 細胞膜脂質非対称センシング機構と細胞応答 Invited

    小原圭介、西野佳菜子、内堀健矢、山内佐織、木原章雄

    第89回日本生化学会大会  2016.9 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:宮城県仙台市   Country:Japan  

  25. ユビキチンとESCRT複合体による細胞膜脂質非対称および外界アルカリ化シグナルの伝達

    小原圭介、西野佳菜子、木原章雄

    第67回日本細胞生物学会大会  2015.6 

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    Event date: 2015.6 - 2015.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京都   Country:Japan  

  26. 脂質非対称センサーの解剖 Invited

    8) 小原圭介

    第187回酵母細胞研究会  2014.12.12 

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    Event date: 2014.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東京都   Country:Japan  

  27. Identification of the sensor protein detecting lipid asymmetry in the plasma membrane Invited

    Obara K, Yamamoto H and Kihara A

    第65回日本細胞生物学会大会  2013.6 

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    Event date: 2013.6

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Venue:愛知県名古屋市   Country:Japan  

  28. 細胞膜脂質非対称センサーの同定

    小原圭介、山本林、木原章雄

    第65回日本細胞生物学会大会  2013.6 

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    Event date: 2013.6

    Language:Japanese  

    Venue:愛知県名古屋市   Country:Japan  

  29. 小胞輸送における極長鎖脂肪酸の機能解析

    小原圭介、小島亮、木原章雄

    酵母遺伝学フォーラム第45回研究報告会  2012.9 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都府京都市   Country:Japan  

  30. 脂質非対称の乱れに対する細胞応答の網羅的解析

    小原圭介、山内佐織、神村章子、安住薫、木原章雄

    第34回日本分子生物学会年会  2011.12 

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    Event date: 2011.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神奈川県横浜市   Country:Japan  

  31. 窒素飢餓応答におけるスフィンゴ脂質の役割

    山形麻旗、小原圭介、木原章雄

    日本薬学会北海道支部例会第134回例会  2010.5.8 

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    Event date: 2010.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北海道札幌市   Country:Japan  

  32. 脂質非対称変化による酵母スフィンゴイド塩基トランスロカーゼ発現誘導の分子機構 Invited

    木原章雄、池田未佳、小原圭介、五十嵐靖之

    第31回日本分子生物学会年会・第81回日本生化学会大会合同大会  2008.12 

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    Event date: 2008.12

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:兵庫県神戸市   Country:Japan  

  33. オートファジーにおけるPtdIns(3)Pの動態と機能

    小原圭介、野田健司、関藤孝之、新實香緒里、大隅良典

    酵母遺伝学フォーラム第41回研究報告会  2008.9 

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    Event date: 2008.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北海道札幌市   Country:Japan  

  34. PtdIns 3-kinaseが演出するオートファゴソーム形成 Invited

    小原圭介、関藤孝之、新實香緒里、野田健司、木原章雄、大隅良典

    日本生化学会北海道支部第45回例会  2008.8.8 

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    Event date: 2008.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:北海道札幌市   Country:Japan  

  35. 管状要素特異的新規ポリガラクツロナーゼの解析

    小澤靖子、小原圭介、出村拓、福田裕穂

    第46回日本植物生理学会  2005.3 

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    Event date: 2005.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:新潟県新潟市   Country:Japan  

  36. イネのモノデヒドロアスコルビン酸還元酵素(MDAR)の択一的スプライシングとオルガネラ二重移行について

    小原圭介、福田裕穂

    第45回日本植物生理学会  2004.3 

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    Event date: 2004.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京都   Country:Japan  

  37. モノデヒドロアスコルビン酸還元酵素のオルガネラ二重移行機構 Invited

    小原圭介、福田裕穂

    植物オルガネラワークショップ「植物オルガネラ研究の新局面」  2004.3.26 

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    Event date: 2004.3

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

    Venue:東京都   Country:Japan  

  38. 管状要素のプログラム細胞死および分化初期過程でのカルモジュリンの働き

    小原圭介、中名生幾子、吉良拡、福田裕穂

    日本植物学会第67回大会  2003.9 

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    Event date: 2003.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北海道札幌市   Country:Japan  

  39. シロイヌナズナのMDAR遺伝子は転写開始点の使い分けによりミトコンドリア(Mt)型、葉緑体型(Cp)酵素を作り分ける

    小原圭介、鷲見和良、福田裕穂

    第44回日本植物生理学会  2003.3 

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    Event date: 2003.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:奈良県奈良市   Country:Japan  

  40. The vacuole is a key player of developmentally programmed cell death in plants. Invited International conference

    Fukuda H, Yamamoto R, Obara K, Kuriyama H and Ito J

    A joint meeting of the German Society for Cell Biology and the German Society for Developmental Biology  2003.3.27 

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    Event date: 2003.3

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Venue:Bonn   Country:Germany  

  41. 管状要素分化に伴うプログラム細胞死の観察

    小原圭介、福田裕穂

    第41回日本植物生理学会  2000.3 

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    Event date: 2000.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:愛知県名古屋市   Country:Japan  

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Works 2

  1. 生化学辞典第4版

    2007

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    細胞内膜動態に関する約15項目を執筆

  2. 植物の生化学・分子生物学

    2005

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    第5章「細胞骨格」の章を英語から日本語に翻訳

KAKENHI (Grants-in-Aid for Scientific Research) 11

  1. 細胞膜との相互作用を介した脂質非対称センシングの分子機構

    Grant number:22K06141  2022.4 - 2025.3

    科学研究費助成事業  基盤研究(C)

    小原 圭介

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    Authorship:Principal investigator 

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

    細胞膜の脂質二重層では内外層で脂質組成や役割が大きく異なる。その様な「脂質非対称」は細胞の生存に必須である。申請者は、脂質非対称の状態をモニターするセンサータンパク質Rim21を出芽酵母で同定した。本研究では、Rim21が脂質非対称の状態をモニターする分子機構の解明を行う。また、申請者はRim21を利用して、生きた細胞で脂質非対称の状態をモニターできる脂質非対称バイオセンサーの開発も行っており、本研究で有用な知見が得られる。本研究の推進により、脂質とタンパク質の相互作用に関する新たなパラダイムが提供できるほか、真菌感染症の予防や治療に寄与する知見が得られると考えられる。
    細胞膜の脂質二重層では、内外層間で脂質分子が不均一に分布している。この様な「脂質非対称」は真核生物に共通の性質であり、この維持や調節が大きく破綻すると細胞一つとして生存できない。研究代表者は、過去に脂質非対称の状態変化を感知して適応反応を引き起こす脂質非対称センサータンパク質Rim21を出芽酵母で同定している。本研究では、Rim21が脂質非対称変化を感知し、下流因子にシグナルを出力する分子機構の解明を試みた。
    今年度は、脂質非対称センサーモチーフを内包するRim21のC末端細胞質領域(Rim21C)の組換えタンパク質と脂質との相互作用を調べた。その結果、Rim21Cはホスファチジルセリンやホスファチジン酸などの負電荷を有する脂質に親和性を示し、それらの脂質の脱プロトン化を促す効果があるホスファチジルエタノールアミンがその親和性を高めることを見出した。
    また、Rim21が発する脂質非対称シグナル伝達にはユビキチンリガーゼであるRsp5によるユビキチン化が必須である。本研究では、そのユビキチン化に関わるRim21の領域やユビキチン化部位などを調査した。その結果、Rim21C内のPxxYモチーフが脂質非対称シグナル伝達に必要であることが明らかになった。PxxYモチーフはRsp5がユビキチン化のターゲットを認識して結合する際にしばしば用いられるモチーフである。Rsp5のターゲットの少なくとも1つがRim21である可能性を示す結果である。一方、Rsp5が脂質非対称シグナル伝達を媒介するのに必要な領域についても調査を開始した。
    Rim21Cと脂質との相互作用の解析には、RIm21Cの組換えタンパク質が必要である。しかし、大腸菌を用いたタンパク質発現系では、Rim21C組換えタンパク質はほとんど発現せず、十分な量が確保できなかった。そこで、様々なタンパク質発現系を試し、最終的にコムギ胚芽抽出液を用いたin vitro転写・翻訳系により、ある程度の量を精製することが出来た。しかし、大規模な実験にはまだ十分ではない。この点がネックになり、Rim21Cと脂質との相互作用の解析には遅れが生じている。
    一方、Rim21が下流の夫因子にシグナルを出力する仕組みに関しては、Rim21内の重要なモチーフを新たに見出すことが出来た。また、ユビキチン化を担うRsp5のターゲット認識部位の調査も開始した。Rsp5は機能を失うと細胞が致死になる必須タンパク質であるため、生存に必要な機能を保ちながら、脂質非対称シグナルのみを停止することが出来る点変異体が必要であった。その様な株の構築に苦労したが進展を見た。
    総合すると、前進した部分もあるものの停滞している部分もあり、やや遅れているとの判断に至った。
    引き続き、Rim21Cと脂質の相互作用の解析および下流因子へのシグナル出力機構の解析を行う。
    前者については、当面はコムギ胚芽抽出液を用いたin vitro転写・翻訳系を用いてRim21C組換えタンパク質の精製を行いながら、並行してより高収量で精製できるシステムを探索する。十分量の組換えタンパク質を調整できたら、脂質オーバーレイアッセイや人工膜小胞リポソームを用いたpull-dpwnアッセイなどで、脂質との相互作用を調査する。必要に応じて、Rim21C部分に変異を導入した変異組換えタンパク質も精製し、同様にアッセイを行うことで、脂質との相互作用に関わるアミノ酸残基を明らかにしていく。
    下流因子へのシグナル出力機構に関しては、今年度に明らかにしたRim21内の重要なモチーフであるPxxYモチーフに変異を導入した株を用いて、シグナル伝達のどの素過程が停止しているかを調べる。これにより、PxxYモチーフが機能する素過程を明らかにする。その他、Rsp5によるユビキチン化のターゲットタンパク質やユビキチン化部位の探索、Rsp5がターゲットを認識する際に用いるドメインの解明などを行う。

  2. Sensing mechanism and signal output of plasma membrane lipid asymmetry

    Grant number:19K06561  2019.4 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Obara Keisuke

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    Authorship:Principal investigator 

    Grant amount:\4420000 ( Direct Cost: \3400000 、 Indirect Cost:\1020000 )

    We investigated into the mechanism of lipid asymmetry sensing by the sensor protein Rim21 and how activated Rim21 evokes signal transduction pathway. As a result, we suggested that some charged amino acid residues within and near the "sensor motif" which is located in the C-terminal cytosolic region of Rim21 (Rim21C) play pivotal roles in interaction between the plasma membrane inner leaflet and Rim21C. This is a great progress that may open the door for elucidation of molecular mechanism underlying lipid asymmetry sensing.
    We also found that alterations in some physicochemical variants outside the cell, e.g. pH and salt concentration, may affect the state of lipid asymmetry. Of them at least pH elevation and high salt stress, was found to be sensed by Rim21 through alterations in lipid asymmetry. This finding deserves attention in that it sheds light on a novel aspect of the plasma membrane as a sensor structure for physicochemical variants.

  3. Development of a lipid asymmetry biosensor that dramatically facilitates studies on biomembranes

    Grant number:18K19292  2018.6 - 2020.3

    Grant-in-Aid for Challenging Research (Exploratory)

    Kamura Takumi

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    Authorship:Coinvestigator(s) 

    In this study, we tried to develop a biosensor that can report the state of lipid asymmetry in the plasma membrane. For this purpose, we focused on and utilized a lipid asymmetry sensor protein Rim21 which we previously identified in yeast. As a result, we developed a prototype of lipid asymmetry biosensor that can visualize alterations in lipid asymmetry in living yeast cells. In addition, we conducted a systematic mutagenesis approach of the prototype biosensor, and succeeded in developing a series of improved biosensors with higher S/N ratio.
    Using these biosensors, we monitored the state of lipid asymmetry in yeast cells exposed to environmental stresses. Interestingly, the state of lipid symmetry seemed to be altered under alkaline and salt stresses. Cells deleted for RIM21 were hypersensitive to alkaline and salt stresses, suggesting that these environmental stresses are sensed by Rim21 through alterations in the state of lipid asymmetry in the plasma membrane.

  4. Elucidation of sensing mechanism of lipid asymmetry and establishment of bases for its application

    Grant number:16K07288  2016.4 - 2020.3

    Obara Keisuke

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    Authorship:Principal investigator 

    Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )

    In this study, we focused on Rim21, the sensor protein for altered lipid asymmetry in the plasma membrane. We suggested that Rim21 senses alterations in lipid asymmetry through interaction between lipid asymmetry sensor motifs in Rim21 and acidic lipids in the plasma membrane. We also elucidated the function of N-glycosylation of Rim21.
    A biosensor that can report the state of lipid asymmetry in living yeast cells was developed in this study. Moreover, we succeeded in improving the S/N ratio of this prototype biosensor by mutagenesis approach.
    Rim21 invokes a signal transduction pathway called the Rim101 pathway, and this pathway is a good potential drug target. We revealed that activation of this pathway is regulated by antagonistic action of ubiquitination and deubiquitination. In addition, we elucidated the mechanism and the biological significance of attenuation of the Rim101 pathway.

  5. 乱れた脂質非対称の感知および修繕機構

    Grant number:25440038  2013.4 - 2016.3

    日本学術振興会  基盤研究(C)

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\5330000 ( Direct Cost: \4100000 、 Indirect Cost:\1230000 )

  6. 脂質非対称を感知して伝達する分子機構の解明

    Grant number:25650059  2013.4 - 2015.3

    挑戦的萌芽研究

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type:Competitive

  7. 極長鎖脂肪酸産生の分子メカニズムと病態,生理機能の解明

    Grant number:23370057  2011.4 - 2014.3

    基盤研究(B)

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type:Competitive

  8. 脂質非対称センシングの分子機構と細胞応答

    Grant number:23770135  2011.4 - 2013.3

    日本学術振興会  若手研究(B)

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    Authorship:Principal investigator  Grant type:Competitive

  9. 脂質非対称を感知するセンサーと細胞内シグナルの分子機構の解明

    Grant number:23657120  2011.4 - 2013.3

    挑戦的萌芽研究

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type:Competitive

  10. 脂質分子から迫るオートファゴソーム形成機構の研究

    Grant number:21770129  2009.4 - 2011.3

    日本学術振興会  若手研究(B)

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\4550000 ( Direct Cost: \3500000 、 Indirect Cost:\1050000 )

  11. Phs1ファミリーによるホスファチジルイノシトールの細胞内輸送機構の解明

    Grant number:20687008  2008.4 - 2011.3

    若手研究(A)

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type:Competitive

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Teaching Experience (On-campus) 3

  1. 生物科学実験法及び実験VI

    2023

     詳細を見る

    学部2年生の学生に対して、大腸菌の増殖と形質転換の学生実習を行った。
    学部2年生の学生に対して、試験管内ユビキチン修飾反応の学生実習を行った。
    共にレポート作成の補助や添削、採点、フィードバックを行った。

  2. 基礎生化学IIIb

    2019

  3. 基礎生化学III

    2019