研究者詳細 - 井上　愛子

写真a

イノウエ　アイコ

井上　愛子

INOUE Aiko

所属

未来社会創造機構ナノライフシステム研究所マイクロ・ナノメカトロニクス研究部門特任講師
大学院医学系研究科特任講師

学位 1

博士（医学）（ 2017年3月名古屋大学）

学位の先頭へ▲

学歴 2

名古屋大学大学院医学系研究科　総合医学専攻博士課程

- 2017年3月

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国名：日本国
浜松医科大学大学院医学系研究科看護学専攻修士課程

- 2003年3月

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国名：日本国

学歴の先頭へ▲

所属学協会 2

日本サルコペニア・フレイル学会評議員
日本老年医学会

所属学協会の先頭へ▲

委員歴 4

豊山町地域包括支援センター運営協議会委員

2121年8月
豊山町健康づくり審議会委員

2022年7月
豊山町地域包括支援センター豊山町地域包括ケアシステム推進協議会

2022年7月

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団体区分：自治体
豊山町総合計画審議会委員

2018年11月

委員歴の先頭へ▲

受賞 2

第4回日本サルコペニア・フレイル学会大会最優秀演題賞

2017年10月第4回日本サルコペニア・フレイル学会大会

井上愛子、成憲武、朴麗梅、五藤大貴、小笠原真雄、葛谷雅文

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受賞区分：国内学会・会議・シンポジウム等の賞受賞国：日本国
第3回日本自律神経学会賞（基礎部門優秀論文賞）

2006年11月日本自律神経学会食事摂取と体位が健常者の核心温ならびに血圧に及ぼす影響―炭水化物摂取と仰臥位・長坐位における検討―

長谷川愛子、伊藤剛、石津みゑ子、花輪壽彦

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受賞区分：国内学会・会議・シンポジウム等の賞受賞国：日本国

受賞の先頭へ▲

論文 57

Young bone marrow transplantation prevents aging-related muscle atrophy in a senescence-accelerated mouse prone 10 model

Inoue Aiko, Piao Limei, Yue Xueling, Huang Zhe, Hu Lina, Wu Hongxian, Meng Xiangkun, Xu Wenhu, Yu Chenglin, Sasaki Takeshi, Itakura Kohji, Umegaki Hiroyuki, Kuzuya Masafumi, Cheng Xian Wu

JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE 2022年9月

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記述言語：日本語出版者・発行元：Journal of Cachexia, Sarcopenia and Muscle

Background: Young bone marrow transplantation (YBMT) has been shown to stimulate vascular regeneration in pathological conditions, including ageing. Here, we investigated the benefits and mechanisms of the preventive effects of YBMT on loss of muscle mass and function in a senescence-associated mouse prone 10 (SAMP10) model, with a special focus on the role of growth differentiation factor 11 (GDF-11). Methods: Nine-week-old male SAMP10 mice were randomly assigned to a non-YBMT group (n = 6) and a YBMT group (n = 7) that received the bone marrow of 8-week-old C57BL/6 mice. Results: Compared to the non-YBMT mice, the YBMT mice showed the following significant increases (all P < 0.05 in 6–7 mice): endurance capacity (>61.3%); grip strength (>37.9%), percentage of slow myosin heavy chain fibres (>14.9–15.9%). The YBMT also increased the amounts of proteins or mRNAs for insulin receptor substrate 1, p-Akt, p-extracellular signal-regulated protein kinase1/2, p-mammalian target of rapamycin, Bcl-2, peroxisom proliferator-activated receptor-γ coactivator (PGC-1α), plus cytochrome c oxidase IV and the numbers of proliferating cells (n = 5–7, P < 0.05) and CD34+/integrin-α7+ muscle stem cells (n = 5–6, P < 0.05). The YMBT significantly decreased the levels of gp91phox, caspase-9 proteins and apoptotic cells (n = 5–7, P < 0.05) in both muscles; these beneficial changes were diminished by the blocking of GDF-11 (n = 5–6, P < 0.05). An administration of mouse recombinant GDF-11 improved the YBMT-mediated muscle benefits (n = 5–6, P < 0.05). Cell therapy with young bone marrow from green fluorescent protein (GFP) transgenic mice exhibited GFP+ myofibres in aged muscle tissues. Conclusions: These findings suggest that YBMT can prevent muscle wasting and dysfunction by mitigating apoptosis and proliferation via a modulation of GDF-11 signalling and mitochondrial dysfunction in SAMP10 mice.

DOI： 10.1002/jcsm.13058

Web of Science

Scopus

PubMed
Cathepsin K Deficiency Prevented Kidney Damage and Dysfunction in Response to 5/6 Nephrectomy Injury in Mice With or Without Chronic Stress

Yue Xueling, Piao Limei, Wang Hailong, Huang Zhe, Meng Xiangkun, Sasaki Takeshi, Inoue Aiko, Nakamura Kae, Wan Ying, Xu Shengnan, Shi Guo-Ping, Kim Weon, Murohara Toyoaki, Kuzuya Masafumi, Cheng Xian Wu

HYPERTENSION 79 巻 ( 8 ) 頁： 1713 - 1723 2022年8月

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記述言語：日本語出版者・発行元：Hypertension

Background: Chronic psychological stress is a risk factor for kidney disease, including kidney dysfunction and hypertension. Lysosomal CatK (cathepsin K) participates in various human pathobiologies. We investigated the role of CatK in kidney remodeling and hypertension in response to 5/6 nephrectomy injury in mice with or without chronic stress. Methods: Male 7-week-old WT (wild type; CatK+/+) and CatK-deficient (CatK-/-) mice that were or were not subjected to chronic stress underwent 5/6 nephrectomy. At 8 weeks post-stress/surgery, the stress was observed to have accelerated injury-induced glomerulosclerosis, proteinuria, and blood pressure elevation. Results: Compared with the nonstressed mice, the stressed mice showed increased levels of TLR (Toll-like receptor)-2/4, p22phox, gp91phox, CatK, MMP (matrix metalloproteinase)-2/9, collagen type I and III genes, PPAR-γ (peroxisome proliferator-activated receptor-gamma), NLRP-3 (NOD-like receptor thermal protein domain associated protein 3), p21, p16, and cleaved caspase-8 proteins, podocyte foot process effacement, macrophage accumulation, apoptosis, and decreased levels of Bcl-2 (B cell lymphoma 2) and Sirt1, as well as decreased glomerular desmin expression in the kidneys. These harmful changes were retarded by the genetic or pharmacological inhibition of CatK. Consistently, CatK inhibition ameliorated 5/6 nephrectomy-related kidney injury and dysfunction. In mesangial cells, CatK silencing or overexpression, respectively, reduced or increased the PPAR-γ and cleaved caspase-8 protein levels, providing evidence and a mechanistic explanation of CatK's involvement in PPAR-γ/caspase-8-mediated cell apoptosis in response to superoxide and stressed serum. Conclusions: These results demonstrate that CatK plays an essential role in kidney remodeling and hypertension in response to 5/6 nephrectomy or stress, possibly via a reduction of glomerular inflammation, apoptosis, and fibrosis, suggesting a novel therapeutic strategy for controlling kidney injury in mice under chronic psychological stress conditions.

DOI： 10.1161/HYPERTENSIONAHA.122.19137

Web of Science

Scopus

PubMed
Human umbilical cord-derived mesenchymal stromal cells ameliorate aging-associated skeletal muscle atrophy and dysfunction by modulating apoptosis and mitochondrial damage in SAMP10 mice

Piao Limei, Huang Zhe, Inoue Aiko, Kuzuya Masafumi, Cheng Xian Wu

STEM CELL RESEARCH & THERAPY 13 巻 ( 1 ) 頁： 226 2022年6月

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記述言語：日本語出版者・発行元：Stem Cell Research and Therapy

Background: Skeletal muscle mass and function losses in aging individuals are associated with quality of life deterioration and disability. Mesenchymal stromal cells exert immunomodulatory and anti-inflammatory effects and could yield beneficial effects in aging-related degenerative disease. Methods and results: We investigated the efficacy of umbilical cord-derived mesenchymal stromal cells (UC-MSCs) on sarcopenia-related skeletal muscle atrophy and dysfunction in senescence-accelerated mouse prone 10 (SAMP10) mice. We randomly assigned 24-week-old male SAMP10 mice to a UC-MSC treatment group and control group. At 12 weeks post-injection, the UC-MSC treatment had ameliorated sarcopenia-related muscle changes in performance, morphological structures, and mitochondria biogenesis, and it enhanced the amounts of proteins or mRNAs for myosin heavy chain, phospho-AMP-activated protein kinase, phospho-mammalian target of rapamycin, phospho-extracellular signal-regulated kinase1/2, peroxisome proliferator-activated receptor-γ coactivator, GLUT-4, COX-IV, and hepatocyte growth factor in both gastrocnemius and soleus muscles, and it reduced the levels of proteins or mRNAs for cathepsin K, cleaved caspase-3/-8, tumor necrosis factor-α, monocyte chemoattractant protein-1, and gp91phox mRNAs. The UC-MSC treatment retarded mitochondria damage, cell apoptosis, and macrophage infiltrations, and it enhanced desmin/laminin expression and proliferating and CD34+/Integrin α7+ cells in both types of skeletal muscle of the SAMP10 mice. In vitro, we observed increased levels of HGF, PAX-7, and MoyD mRNAs at the 4th passage of UC-MSCs. Conclusions: Our results suggest that UC-MSCs can improve sarcopenia-related skeletal muscle atrophy and dysfunction via anti-apoptosis, anti-inflammatory, and mitochondrial biogenesis mechanisms that might be mediated by an AMPK-PGC1-α axis, indicating that UC-MSCs may provide a promising treatment for sarcopenia/muscle diseases.

DOI： 10.1186/s13287-022-02895-z

Web of Science

Scopus

PubMed
Cathepsin K activity controls cachexia-induced muscle atrophy via the modulation of IRS1 ubiquitination

Meng Xiangkun, Huang Zhe, Inoue Aiko, Wang Hailong, Wan Ying, Yue Xueling, Xu Shengnan, Jin Xueying, Shi Guo-Ping, Kuzuya Masafumi, Cheng Xian Wu

JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE 13 巻 ( 2 ) 頁： 1197 - 1209 2022年4月

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記述言語：日本語

DOI： 10.1002/jcsm.12919

Web of Science
Short- and long-term effects of different exercise programs on the gait performance of older adults with subjective cognitive decline: A randomized controlled trial

Fujita Kosuke, Umegaki Hiroyuki, Makino Taeko, Uemura Kazuki, Hayashi Takahiro, Inoue Aiko, Uno Chiharu, Kitada Tomoharu, Huang Chi Hsien, Shimada Hiroyuki, Kuzuya Masafumi

EXPERIMENTAL GERONTOLOGY 156 巻 2021年12月

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記述言語：日本語

DOI： 10.1016/j.exger.2021.111590

Web of Science
Proliferin-1 Ameliorates Cardiotoxin-Related Skeletal Muscle Repair in Mice

Goto Hiroki, Inoue Aiko, Piao Limei, Hu Lina, Huang Zhe, Meng Xiangkun, Suzuki Yusuke, Umegaki Hiroyuki, Kuzuya Masafumi, Cheng Xian Wu

STEM CELLS INTERNATIONAL 2021 巻 2021年11月

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記述言語：日本語

DOI： 10.1155/2021/9202990

Web of Science
Sequestration of RBM10 in Nuclear Bodies: Targeting Sequences and Biological Significance

Wang Ling-Yu, Xiao Sheng-Jun, Kunimoto Hiroyuki, Tokunaga Kazuaki, Kojima Hirotada, Kimura Masatsugu, Yamamoto Takahiro, Yamamoto Naoki, Zhao Hong, Nishio Koji, Tani Tokio, Nakajima Koichi, Sunami Kishiko, Inoue Akira

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22 巻 ( 19 ) 2021年10月

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記述言語：日本語出版者・発行元：International Journal of Molecular Sciences

RBM10 is an RNA-binding protein that regulates alternative splicing (AS). It localizes to the extra-nucleolar nucleoplasm and S1-1 nuclear bodies (NBs) in the nucleus. We investigated the biological significance of this localization in relation to its molecular function. Our analyses, employing deletion mutants, revealed that RBM10 possesses two S1-1 NB-targeting sequences (NBTSs), one in the KEKE motif region and another in the C2H2 Zn finger (ZnF). These NBTSs act synergistically to localize RBM10 to S1-1 NBs. The C2H2 ZnF not only acts as an NBTS, but is also essential for AS regulation by RBM10. Moreover, RBM10 does not participate in S1-1 NB formation, and without alterations of RBM10 protein levels, its NB-localization changes, increasing as cellular transcriptional activity declines, and vice versa. These results indicate that RBM10 is a transient component of S1-1 NBs and is sequestered in NBs via its NBTSs when cellular transcription decreases. We propose that the C2H2 ZnF exerts its NB-targeting activity when RBM10 is unbound by pre-mRNAs, and that NB-localization of RBM10 is a mechanism to control its AS activity in the nucleus.

DOI： 10.3390/ijms221910526

Web of Science

Scopus
Statins Mitigate Stress-Related Vascular Aging and Atherosclerosis in apoE-Deficient Mice Fed High Fat-Diet: The Role of Glucagon-Like Peptide-1/Adiponectin Axis

Lei Yanna, Cui Qingsong, Yang Guang, Piao Limei, Inoue Aiko, Wu Hongxian, Li Xiang, Kuzuya Masafumi, Cheng Xian Wu

FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY 9 巻 2021年7月

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記述言語：日本語

DOI： 10.3389/fcell.2021.687868

Web of Science
Effect of Various Exercises on Intrinsic Capacity in Older Adults With Subjective Cognitive Concerns

Huang Chi Hsien, Umegaki Hiroyuki, Makino Taeko, Uemura Kazuki, Hayashi Takahiro, Kitada Tomoharu, Inoue Aiko, Shimada Hiroyuki, Kuzuya Masafumi

JOURNAL OF THE AMERICAN MEDICAL DIRECTORS ASSOCIATION 22 巻 ( 4 ) 頁： 780 - + 2021年4月

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記述言語：日本語

DOI： 10.1016/j.jamda.2020.06.048

Web of Science
Mental Health Status of the Older Adults in Japan During the COVID-19 Pandemic

Fujita Kosuke, Inoue Aiko, Kuzuya Masafumi, Uno Chiharu, Huang Chi Hsien, Umegaki Hiroyuki, Onishi Joji

JOURNAL OF THE AMERICAN MEDICAL DIRECTORS ASSOCIATION 22 巻 ( 1 ) 頁： 220 - 221 2021年1月

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記述言語：日本語

DOI： 10.1016/j.jamda.2020.11.023

Web of Science
Effect of various exercises on frailty among older adults with subjective cognitive concerns: a randomised controlled trial

Huang Chi Hsien, Umegaki Hiroyuki, Makino Taeko, Uemura Kazuki, Hayashi Takahiro, Kitada Tomoharu, Inoue Aiko, Shimada Hiroyuki, Kuzuya Masafumi

AGE AND AGEING 49 巻 ( 6 ) 頁： 1011 - 1019 2020年11月

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記述言語：日本語

DOI： 10.1093/ageing/afaa086

Web of Science
Increased dipeptidyl peptidase-4 accelerates chronic stress-related thrombosis in a mouse carotid artery model

Jin Xianglan, Jin Chunzi, Nakamura Kae, Jin Tiefeng, Xin Minglong, Wan Ying, Yue Xueling, Jin Shengyu, Wang Hailong, Inoue Aiko, Nan Yongshan, Lin Zhenhua, Kuzuya Masafumi, Cheng Xian Wu

JOURNAL OF HYPERTENSION 38 巻 ( 8 ) 頁： 1504 - 1513 2020年8月

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記述言語：日本語

DOI： 10.1097/HJH.0000000000002418

Web of Science
Deficiency of cysteinyl cathepsin K suppresses the development of experimental intimal hyperplasia in response to chronic stress

Meng Xiangkun, Piao Limei, Wang Hailong, Inoue Aiko, Huang Zhe, Jiang Haiying, Nakamura Kae, Sasaki Takeshi, Li Xiang, Xu Wenhu, Yu Chenglin, Hu Lina, Wu Hongxian, Murohara Toyoaki, Shi Guo-Ping, Kuzuya Masafumi, Cheng Xian Wu

JOURNAL OF HYPERTENSION 38 巻 ( 8 ) 頁： 1514 - 1524 2020年8月

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記述言語：日本語

DOI： 10.1097/HJH.0000000000002424

Web of Science
Combined Impact of Physical Frailty and Social Isolation on Rate of Falls in Older Adults

Hayashi T., Umegaki Hiroyuki, Makino T., Huang C. H., Inoue A., Shimada H., Kuzuya M.

JOURNAL OF NUTRITION HEALTH & AGING 24 巻 ( 3 ) 頁： 312 - 318 2020年3月

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記述言語：日本語

DOI： 10.1007/s12603-020-1316-5

Web of Science
PLF-1 (Proliferin-1) Modulates Smooth Muscle Cell Proliferation and Development of Experimental Intimal Hyperplasia

Hu Lina, Huang Zhe, Ishii Hideki, Wu Hongxian, Suzuki Susumu, Inoue Aiko, Kim Weon, Jiang Haiying, Li Xiang, Zhu Enbo, Piao Limei, Zhao Guangxian, Lei Yanna, Okumura Kenji, Shi Guo-Ping, Murohara Toyoaki, Kuzuya Masafumi, Cheng Xian Wu

JOURNAL OF THE AMERICAN HEART ASSOCIATION 8 巻 ( 24 ) 2019年12月

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記述言語：日本語

DOI： 10.1161/JAHA.117.005886

Web of Science
Cathepsin S-Mediated Negative Regulation of Wnt5a/SC35 Activation Contributes to Ischemia-Induced Neovascularization in Aged Mice

Xu Wenhu, Yu Chenglin, Piao Limei, Inoue Aiko, Wang Hailong, Meng Xiangkun, Li Xiang, Cui Lan, Umegaki Hiroyuki, Shi Guo-Ping, Murohara Toyoaki, Kuzuya Masafumi, Cheng Xian Wu

CIRCULATION JOURNAL 83 巻 ( 12 ) 頁： 2537 - + 2019年12月

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記述言語：日本語

DOI： 10.1253/circj.CJ-19-0325

Web of Science
Cathepsin S Deficiency Mitigated Chronic Stress-Related Neointimal Hyperplasia in Mice

Wang Hailong, Meng Xiangkun, Piao Limei, Inoue Aiko, Xu Wenhu, Yu Chenglin, Nakamura Kae, Hu Lina, Sasaki Takeshi, Wu Hongxian, Unno Kazumasa, Umegaki Hiroyuki, Murohara Toyoaki, Shi Guo-Ping, Kuzuya Masafumi, Cheng Xian Wu

JOURNAL OF THE AMERICAN HEART ASSOCIATION 8 巻 ( 14 ) 2019年7月

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記述言語：日本語

DOI： 10.1161/JAHA.119.011994

Web of Science
Cathepsin S Deficiency Impaired Noevascularization in Response to Ischemia via the Activation of Wnt5-SC35 Signaling Pathway in Advanced Age

Xu Wenhu, Yu Chenglin, Piao Limei, Inoue Aiko, Kuzuya Masafumi, Cheng Xian Wu

CIRCULATION 138 巻 2018年11月

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記述言語：日本語

Web of Science
ACDF-1Modulates Smooth Muscle Cell Proliferation and Development of Experimental Intimal Hyperplasia

Hu Lina, Ishii Hideki, Wu Hongxian, Suzuki Susumu, Inoue Aiko, Shi Guo-Ping, Murohara Toyoaki, Kuzuya Masafumi, Cheng Xian Wu

CIRCULATION 138 巻 2018年11月

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記述言語：日本語

Web of Science
Adiponectin/AdipoR1 Signal Inactivation Contributes to Impaired Angiogenesis in Mice of Advanced Age

Piao Limei, Yu Chenglin, Xu Wenhu, Inoue Aiko, Shibata Rei, Ouchi Noriyuki, Murohara Toyoaki, Kuzuya Masafumi, Cheng Xian Wu

CIRCULATION 138 巻 2018年11月

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記述言語：日本語

Web of Science
Apoptotic Cell-Derived Growth Factor-1 Modulates Noevascularization in Response to Ischemia via the activation of PI3K/Akt/p38MAPK-Depended and -Independent mTOR Signaling Cascades

Yu Chenglin, Piao Limei, Xu Wenhu, Inoue Aiko, Kuzuya Masafumi, Cheng Xian Wu

CIRCULATION 138 巻 2018年11月

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記述言語：日本語

Web of Science
Cathepsin S Controls Injury-Related Neointimal Formation in Mice Under Chronic Stress via the Modulation of Inflammation and Immune Action

Wang Hailong, Li Limei, Meng Xiangkun, Inoue Aiko, Shi Guo-Ping, Kuzuya Masafumi, Cheng Xian Wu

CIRCULATION 138 巻 2018年11月

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記述言語：日本語

Web of Science
Adiponectin/AdiopR1 signal inactivation contributes to impaired angiogenesis in mice of advanced age

Piao Limei, Yu Chenglin, Xu Wenhu, Inoue Aiko, Shibata Rei, Li Xiang, Nan Yongshan, Zhao Guangxian, Wang Hailong, Meng Xiangkun, Lei Yanna, Goto Hiroki, Ouchi Noriyuki, Murohara Toyoaki, Kuzuya Masafumi, Cheng Xian Wu

INTERNATIONAL JOURNAL OF CARDIOLOGY 267 巻頁： 150-155 2018年9月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.ijcard.2018.05.089

Web of Science
Response to letter "DPP-4 inhibition as a therapeutic strategy to ameliorate diabetic metabolic memory"

Cheng Xian Wu, Lei Yanna, Piao Limei, Inoue Aiko, Yang Guang, Zhu Enbo, Kuzuya Masafumi

INTERNATIONAL JOURNAL OF CARDIOLOGY 256 巻頁： 17-17 2018年4月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.ijcard.2017.08.041

Web of Science
Cathepsin K activity controls cardiotoxin-induced skeletal muscle repair in mice

Ogasawara Shinyu, Cheng Xian Wu, Inoue Aiko, Hu Lina, Piao Limei, Yu Chenglin, Goto Hiroki, Xu Wenhu, Zhao Guangxian, Lei Yanna, Yang Guang, Kimura Kaoru, Umegaki Hiroyuki, Shi Guo-Ping, Kuzuya Masafumi

JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE 9 巻 ( 1 ) 頁： 160 - 175 2018年2月

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記述言語：日本語掲載種別：研究論文（学術雑誌）

DOI： 10.1002/jcsm.12248

Web of Science
サルコペニアの分子メカニズム

井上愛子, 成憲武, 五籐大貴, 葛谷雅文

日本老年医学会雑誌 55 巻 ( 1 ) 頁： 13 - 24 2018年

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記述言語：日本語出版者・発行元：一般社団法人日本老年医学会

<p>サルコペニア（sarcopenia）は，加齢に伴うさまざまな要因により，骨格筋蛋タンパクの合成と分解のアンバランス，筋肉修復能低下などが生じることより発症すると考えているが，その詳細に関して多くは不明である．サルコペニアをはじめとした骨格筋萎縮と機能低下の臨床的重要性が認知されつつあるとともに，骨格筋疾患に関する研究も新たな転換期を迎えている．加齢に伴う骨格筋の形態ならびに機能的変化に基づいたオリジナリティーの高い基礎研究が続々と発表され，従来では考えもし得なかった目覚しい発展を遂げている状況である．加齢による骨格筋疾患（サルコペニア）には骨格筋障害を伴っていることがしばしば多く，治療には骨格筋障害へのアプローチが必要である．サルコペニアの発症機序は多因子である．サルコペニア発症・進展プロセスにおいて骨格筋リモデリングと再生不全は重要であり，蛋白質分解システムの異常，そしてタンパク質合成と分解のアンバランスや骨格筋幹細胞の機能不全など様々な要因が関わっている．本稿では，プロテアーゼ·細胞外マトリックス代謝異常，骨格筋タンパク質合成と分解のアンバランス，骨格筋幹細胞老化・機能不全，炎症亢進，骨格筋細胞増殖とアポトーシスアンバランスならびにミトコンドリア機能不全の多方面から前3者に焦点を当てて，本研究グループの研究成果を交えてサルコペニアの新たな分子機構に関する最近の知見について概説する．</p>

DOI： 10.3143/geriatrics.55.13

PubMed

CiNii Research
Chronic Psychological Stress Accelerates Vascular Senescence and Impairs Ischemia-Induced Neovascularization: The Role of Dipeptidyl Peptidase-4/Glucagon-Like Peptide-1-Adiponectin Axis

Piao Limei, Zhao Guangxian, Zhu Enbo, Inoue Aiko, Shibata Rei, Lei Yanna, Hu Lina, Yu Chenglin, Yang Guang, Wu Hongxian, Xu Wenhu, Okumura Kenji, Ouchi Noriyuki, Murohara Toyoaki, Kuzuya Masafumi, Cheng Xian Wu

JOURNAL OF THE AMERICAN HEART ASSOCIATION 6 巻 ( 10 ) 2017年10月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1161/JAHA.117.006421

Web of Science
Increased dipeptidyl peptidase-4 accelerates diet-related vascular aging and atherosclerosis in ApoE-deficient mice under chronic stress

Lei Yanna, Yang Guang, Hu Lina, Piao Limei, Inoue Aiko, Jiang Haiying, Sasaki Takeshi, Zhao Guangxian, Yisireyili Maimaiti, Yu Chenglin, Xu Wenhu, Takeshita Kyosuke, Okumura Kenji, Kuzuya Masafumi, Cheng Xian Wu

INTERNATIONAL JOURNAL OF CARDIOLOGY 243 巻頁： 413-420 2017年9月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.ijcard.2017.05.062

Web of Science
Exenatide mitigated diet-induced vascular aging and atherosclerotic plaque growth in ApoE-deficient mice under chronic stress

Yang Guang, Lei Yanna, Inoue Aiko, Piao Limei, Hu Lina, Jiang Haiying, Sasaki Takeshi, Wu Hongxian, Xu Wenhu, Yu Chenglin, Zhao Guangxian, Ogasawara Shinyu, Okumura Kenji, Kuzuya Masafumi, Cheng Xian-Wu

ATHEROSCLEROSIS 264 巻頁： 1-10 2017年9月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1016/j.atherosclerosis.2017.07.014

Web of Science
Dipeptidyl Peptidase-4 Regulates Hematopoietic Stem Cell Activation in Response to Chronic Stress

Zhu Enbo, Hu Lina, Wu Hongxian, Piao Limei, Zhao Guangxian, Inoue Aiko, Kim Weon, Yu Chenglin, Xu Wenhu, Bando Yasuko K., Li Xiang, Lei Yanna, Hao Chang-Ning, Takeshita Kyosuke, Kim Woo-Shik, Okumura Kenji, Murohara Toyoaki, Kuzuya Masafumi, Cheng Xian Wu

JOURNAL OF THE AMERICAN HEART ASSOCIATION 6 巻 ( 7 ) 2017年7月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1161/JAHA.117.006394

Web of Science
Exercise restores muscle stem cell mobilization, regenerative capacity and muscle metabolic alterations via adiponectin/AdipoR1 activation in SAMP10 mice 査読有り

Aiko Inoue, Xian Wu Cheng, Zhe Huang, Lina Hu, Ryosuke Kikuchi, Haiying Jiang, Limei Piao,Takeshi Sasaki, Kohji Itakura, Hongxian Wu, Guangxian Zhao, Yanna Lei, Guang Yang, Enbo Zhu, Xiang Li, Kohji Sato, Teruhiko Koike, Masafumi Kuzuya.

Journal of Cachexia, Sarcopenia and Muscle 8 巻 ( 3 ) 頁： 370-385 2017年6月

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担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.1002/jcsm.12166.

PubMed
Exercise restores muscle stem cell mobilization, regenerative capacity and muscle metabolic alterations via adiponectin/AdipoR1 activation in SAMP10 mice

Inoue Aiko, Cheng Xian Wu, Huang Zhe, Hu Lina, Kikuchi Ryosuke, Jiang Haiying, Piao Limei, Sasaki Takeshi, Itakura Kohji, Wu Hongxian, Zhao Guangxian, Lei Yanna, Yang Guang, Zhu Enbo, Li Xiang, Sato Kohji, Koike Teruhiko, Kuzuya Masafumi

JOURNAL OF CACHEXIA SARCOPENIA AND MUSCLE 8 巻 ( 3 ) 頁： 370 - 385 2017年6月

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記述言語：日本語掲載種別：研究論文（学術雑誌）

DOI： 10.1002/jcsm.12166

Web of Science
The Soluble VEGF Receptor sFlt-1 Contributes to Impaired Neovascularization in Aged Mice

Zhao Guangxian, Cheng Xian W., Piao Limei, Hu Lina, Lei Yanna, Yang Guang, Inoue Aiko, Ogasawara Shinyu, Wu Hongxian, Hao Chang-Ning, Okumura Kenji, Kuzuya Masafumi

AGING AND DISEASE 8 巻 ( 3 ) 頁： 287-300 2017年6月

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記述言語：英語掲載種別：研究論文（学術雑誌）

DOI： 10.14336/AD.2016.0920

Web of Science
Cathepsin S Activity Controls Injury-Related Vascular Repair in Mice via the TLR2-Mediated p38MAPK and PI3K-Akt/p-HDAC6 Signaling Pathway

Wu Hongxian, Cheng Xian Wu, Hu Lina, Takeshita Kyosuke, Hu Chen, Du Qiuna, Li Xiang, Zhu Enbo, Huang Zhe, Yisireyili Maimaiti, Zhao Guangxian, Piao Limei, Inoue Aiko, Jiang Haiying, Lei Yanna, Zhang Xiaohong, Liu Shaowen, Dai Qiuyan, Kuzuya Masafumi, Shi Guo-Ping, Murohara Toyoaki

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 36 巻 ( 8 ) 頁： 1549 - 1557 2016年8月

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記述言語：日本語出版者・発行元：Arteriosclerosis, Thrombosis, and Vascular Biology

Objective - Cathepsin S (CatS) participates in atherogenesis through several putative mechanisms. The ability of cathepsins to modify histone tail is likely to contribute to stem cell development. Histone deacetylase 6 (HDAC6) is required in modulating the proliferation and migration of various types of cancer cells. Here, we investigated the cross talk between CatS and HADC6 in injury-related vascular repair in mice. Approach and Results - Ligation injury to the carotid artery in mice increased the CatS expression, and CatS-deficient mice showed reduced neointimal formation in injured arteries. CatS deficiency decreased the phosphorylation levels of p38 mitogen-activated protein kinase, Akt, and HDAC6 and toll-like receptor 2 expression in ligated arteries. The genetic or pharmacological inhibition of CatS also alleviated the increased phosphorylation of p38 mitogen-activated protein kinase, Akt, and HDAC6 induced by platelet-derived growth factor BB in cultured vascular smooth muscle cells (VSMCs), and p38 mitogen-activated protein kinase inhibition and Akt inhibition decreased the phospho-HDAC6 levels. Moreover, CatS inhibition caused decrease in the levels of the HDAC6 activity in VSMCs in response to platelet-derived growth factor BB. The HDAC6 inhibitor tubastatin A downregulated platelet-derived growth factor-induced VSMC proliferation and migration, whereas HDAC6 overexpression exerted the opposite effect. Tubastatin A also decreased the intimal VSMC proliferation and neointimal hyperplasia in response to injury. Toll-like receptor 2 silencing decreased the phosphorylation levels of p38 mitogen-activated protein kinase, Akt, and HDAC6 and VSMC migration and proliferation. Conclusions - This is the first report detailing cross-interaction between toll-like receptor 2-mediated CatS and HDAC6 during injury-related vascular repair. These data suggest that CatS/HDAC6 could be a potential therapeutic target for the control of vascular diseases that are involved in neointimal lesion formation.

DOI： 10.1161/ATVBAHA.115.307110

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Cathepsin S activity controls ischemia-induced neovascularization in mice

Li Xiang, Cheng Xian Wu, Hu Lina, Wu Hongxian, Guo-Ping, Hao Chang-Ning, Jiang Haiying, Zhu Enbo, Huang Zhe, Inoue Aiko, Sasaki Takeshi, Du Qiuna, Takeshita Kyosuke, Okumura Kenji, Murohara Toyoaki, Kuzuya Masafumi

INTERNATIONAL JOURNAL OF CARDIOLOGY 183 巻頁： 198 - 208 2015年3月

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記述言語：日本語出版者・発行元：International Journal of Cardiology

Abstract Background Evidence from human and animal studies has demonstrated elevated levels of the cysteine protease cathepsin S (CatS) in hypoxic atherosclerotic lesions. We hypothesized that silencing of CatS gene would suppress ischemia-induced angiogenic action. Methods and results Left femoral artery ligation-induced ischemia in mice showed the increased expression and activity of CatS in the ischemic muscle. The CatS-deficiency (CatS-/-) mice showed impaired functional recovery following hindlimb ischemia and reduced levels of peroxisome proliferator-activated receptor-γ (PPAR-γ), phospho-Akt (p-Akt), p-endothelial nitric oxide synthase, p-extracellular signal-regulated kinase1/2 (Erk1/2), p-p38 mitogen-activated protein kinase, and vascular endothelial growth factor (VEGF) proteins, as well as reduced levels of matrix metalloproteinase-9 and macrophage infiltration in the ischemic muscles. In vitro, CatS silencing reduced the levels of these targeted essential molecules for angiogenesis and vasculogenesis. Together, the results indicated that the effects of CatS knockdown led to defective endothelial cell invasion, proliferation, and tube formation. This notion was reinforced by the finding that CatS inhibition led to a decreased PPAR-γ level and VEGF/Erk1/2 signaling activation in response to ischemia. CatS-/- resulted in decreased circulating EPC-like CD31+/c-Kit+ cells, accompanied by the reduction of the cellular levels of PPAR-γ, p-Akt, and VEGF induced by ischemic stress. Transplantation of bone-marrow-derived mononuclear cells from CatS+/+ mice restored neovascularization in CatS-/- mice. Conclusions CatS activity controls ischemia-induced neovascularization partially via the modulation of PPAR-γ and VEGF/Akt signaling activation.

DOI： 10.1016/j.ijcard.2015.01.058

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Cathepsin K-mediated notch1 activation contributes to neovascularization in response to hypoxia

Jiang Haiying, Cheng Xian Wu, Shi Guo-Ping, Hu Lina, Inoue Aiko, Yamamura Yumiko, Wu Hongxian, Takeshita Kyosuke, Li Xiang, Huang Zhe, Song Haizhen, Asai Masashi, Hao Chang-Ning, Unno Kazumasa, Koike Teruhiro, Oshida Yoshiharu, Okumura Kenji, Murohara Toyoaki, Kuzuya Masafumi

NATURE COMMUNICATIONS 5 巻頁： 3838 2014年6月

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記述言語：日本語出版者・発行元：Nature Communications

Cysteine proteases play important roles in pathobiology. Here we reveal that cathepsin K (CatK) has a role in ischaemia-induced neovascularization. Femoral artery ligation-induced ischaemia in mice increases CatK expression and activity, and CatK-deficient mice show impaired functional recovery following hindlimb ischaemia. CatK deficiency reduces the levels of cleaved Notch1 (c-Notch1), Hes1 Hey1, Hey2, vascular endothelial growth factor, Flt-1 and phospho-Akt proteins of the ischaemic muscles. In endothelial cells, silencing of CatK mimicked, whereas CatK overexpression enhanced, the levels of c-Notch1 and the expression of Notch downstream signalling molecules, suggesting CatK contributes to Notch1 processing and activates downstream signalling. Moreover, CatK knockdown leads to defective endothelial cell invasion, proliferation and tube formation, and CatK deficiency is associated with decreased circulating endothelial progenitor cells-like CD31 + /c-Kit + cells in mice following hindlimb ischaemia. Transplantation of bone marrow-derived mononuclear cells from CatK +/+ mice restores the impairment of neovascularization in CatK -/- mice. We conclude that CatK may be a potential therapeutic target for ischaemic disease. © 2014 Macmillan Publishers Limited.

DOI： 10.1038/ncomms4838

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beta-Hydroxy-beta-methylbutyrate facilitates PI3K/Akt-dependent mammalian target of rapamycin and FoxO1/3a phosphorylations and alleviates tumor necrosis factor alpha/interferon gamma-induced MuRF-1 expression in C2C12 cells

Kimura Kaoru, Cheng Xian Wu, Inoue Aiko, Hu Lina, Koike Teruhiko, Kuzuya Masafumi

NUTRITION RESEARCH 34 巻 ( 4 ) 頁： 368 - 374 2014年4月

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記述言語：日本語出版者・発行元：Nutrition Research

β-Hydroxy- β-methylbutyrate (HMB) prevents deleterious muscle responses under pathological conditions, including tumor- and chronic steroid therapy-related muscle losses. Here, we investigated the hypothesis that HMB may modulate the balance between protein synthesis and degradation in the PI3K/Akt-mediated mammalian target of rapamycin (mTOR) and FoxO1/FoxO3a-dependent mechanisms in differentiated C2C12 muscle cells. We also tested the effect of HMB on the expression of MuRF-1 and atrogin-1 in response to the inflammatory stress. β-Hydroxy- β-methylbutyrate up-regulated phosphorylation of Akt and mTOR, and these effects were completely abolished in the presence of PI3K inhibitor LY294002. β-Hydroxy- β-methylbutyrate also up-regulated FoxO1 and FoxO3a phosphorylation, and these changes were inhibited by LY294002. Although, unexpectedly, HMB failed to reduce the expressions of atrophy-related atrogin-1 messenger RNA and the protein response to the proinflammatory cytokines tumor necrosis factor α plus interferon γ, HMB did attenuate the MuRF-1 expression. Thus, HMB appears to restore the balance between intracellular protein synthesis and proteolysis, likely via activation of the PI3K/Akt-dependent mTOR and FoxO1/FoxO3a signaling pathway and the reduction of tumor necrosis factor α/interferon γ-induced MuRF-1 expression, thereby ameliorating aging-related muscle atrophy. © 2014 Elsevier Inc.

DOI： 10.1016/j.nutres.2014.02.003

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Cathepsin K-Mediated Noich1 Activation Contributes to Neovascularization in Response to Hypoxia

Hu Lina, Cheng Xian Wu, Shi Guo-Ping, Jang Haiying, Inoue Aiko, Yamamura Yumiko, Wu Hongxian, Takeshita Kyosuke, Okumura Kenji, Murohara Toyoaki, Kuzuya Masafumi

CIRCULATION 128 巻 ( 22 ) 2013年11月

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記述言語：日本語

Web of Science
HMG-CoA Reductase Inhibitor Pitavastatin Enhances Renoprotective Effects of Angiotensin Receptor1 Blocker Olmesartan In Salt-Sensitive Hypertensive Rats

Inoue Aiko, Cheng Xian Wu, Sasaki Takeshi, Hu Lina, Jang Haiying, Xiang Li, Kuzuya Masafumi, Murohara Toyoaki, Okumura Kenji

CIRCULATION 128 巻 ( 22 ) 2013年11月

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記述言語：日本語

Web of Science
Akt is essential for adaptive response of systolic left-ventricular function and aging-induced intolerance to exercise

Aoyama M., Bando Y. Kureishi, Monji A., Cheng X. W., Inoue A., Monji A., Mitsui T., Kuzuya M., Murohara T.

EUROPEAN HEART JOURNAL 34 巻頁： 927 - 927 2013年8月

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記述言語：日本語

Web of Science
Mechanisms Underlying the Impairment of Ischemia-Induced Neovascularization in Cathepsin K-Deficient Mic

Jiang Haiying, Cheng Xian Wu, Shi Guo-Ping, Hu Lina, Song Haizhen, Inoue Aiko, Koike Teruhiko, Kuzuya Masafumi

CIRCULATION 126 巻 ( 21 ) 2012年11月

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記述言語：日本語

Web of Science
Statins Enhance the Beneficial Effects of Olmesartan on Renal Injury in Salt-Sensitive Hypertensive Rats via the Reduction of an Angiotensin-Mineralocorticoid Receptor Signaling Interaction

Cheng Xian Wu, Inoue Aiko, Hu Lina, Song Haizhen, Sasaki Takeshi, Kuzuya Masafumi, Okumura Kenji, Murohara Toyoaki

CIRCULATION 124 巻 ( 21 ) 2011年11月

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記述言語：日本語

Web of Science
Angiotensin Type 1 Receptor Blocker Reduces Intimal Neovascularization and Plaque Growth in Apolipoprotein E-Deficient Mice

Cheng Xian Wu, Song Haizhen, Sasaki Takeshi, Hu Lina, Inoue Aiko, Bando Yasuko K., Shi Guo-Ping, Kuzuya Masafumi, Okumura Kenji, Murohara Toyoaki

HYPERTENSION 57 巻 ( 5 ) 頁： 981 - U238 2011年5月

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記述言語：日本語出版者・発行元：Hypertension

The interactions between the renin-angiotensin system and neovascularization in atherosclerotic plaque development are unclear. We investigated the effects of angiotensin II type 1 receptor antagonism in the pathogenesis of atherosclerosis in apolipoprotein E-deficient (ApoE) mice with a special focus on plaque neovascularization. ApoE mice fed a high-fat diet were randomly assigned to 1 of 2 groups and administered vehicle or olmesartan for 12 weeks. Quantification of plaque areas at the aortic root and in the thoracic and abdominal aorta revealed that, in all 3 of the regions, olmesartan reduced intimal neovessel density and the mRNA levels of toll-like receptor (TLR) 2 and TLR4. Olmesartan increased the levels of collagen and elastin, reduced the level of macrophages in the aortic root, and reduced the mRNA and the activity of matrix metalloproteinase (MMP) 2 in aortic roots and thoracic aortas. Aortic ring assay revealed that olmesartan-treated ApoE mice had a markedly lower angiogenic response than that of untreated ApoE mice. Bone marrow-derived endothelial progenitor cell-like c-Kit cells from olmesartan-treated ApoE mice showed marked impairment of cellular functions and lower expression of TLR2/TLR4 and MMP-2 compared with those of untreated controls. MMP-2 deficiency reduced intimal neovessel density and atherosclerotic lesion formation. Olmesartan and small-interfering RNA targeting TLR2 reduced the levels of TLR2, and MMP-2 mRNA induced angiotensin II in cultured endothelial cells. Angiotensin II type 1 receptor antagonism appears to inhibit intimal neovascularization in ApoE mice, partly by reducing TLR2/TLR4-mediated inflammatory action and MMP activation, thus decreasing atherosclerotic plaque growth and increasing plaque instability. © 2011 American Heart Association, Inc.

DOI： 10.1161/HYPERTENSIONAHA.110.168385

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Inhibition of mineralocorticoid receptor is a renoprotective effect of the 3-hydroxy-3-methylglutaryl-coenzyme A reductase inhibitor pitavastatin

Cheng Xian Wu, Kuzuya Masafumi, Sasaki Takeshi, Inoue Aiko, Hu Lina, Song Haizhen, Huang Zhe, Li Ping, Takeshita Kyosuke, Hirashiki Akihiro, Sato Kohji, Shi Guo-Ping, Okumura Kenji, Murohara Toyoaki

JOURNAL OF HYPERTENSION 29 巻 ( 3 ) 頁： 542 - 552 2011年3月

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記述言語：日本語出版者・発行元：Journal of Hypertension

Objective: The mineralocorticoid receptor has been implicated in the pathogenesis of chronic cardiorenal disease. Statins improve renal remodeling and dysfunction in patients with proteinuric kidney diseases. We aimed to clarify the beneficial effects and mechanisms of action of statins in renal insufficiency. Methods and Results: Dahl salt-sensitive rats fed a high-salt diet were treated from 12 to 20 weeks of age with vehicle, the reduced nicotinamide-adenine dinucleotide phosphate (NADPH) oxidase inhibitor apocynin, the synthetic cathepsin inhibitor E64d, or a low or high dosage of pitavastatin (1 or 3 mg/kg daily). Rats fed a low-salt diet served as controls. Rats on the high-salt diet developed massive proteinuria and glomerulosclerosis; these changes were attenuated by both doses of pitavastatin. The amounts of mRNAs or proteins for mineralocorticoid receptor, angiotensin-converting enzyme, angiotensin II type 1 receptor (AT1R), monocyte chemoattractant protein-1, osteopontin, macrophage infiltration, and NADPH subunits (gp91, p22, and Rac1) were significantly higher in the failing kidneys of vehicle-treated rats than in the kidneys of control rats. Either dose of pitavastatin significantly attenuated these changes. These effects of pitavastatin were mimicked by those of apocynin and E64d. Pretreatment with pitavastatin and apocynin inhibited mRNA and protein of mineralocorticoid receptor induced by angiotensin II in cultured podocytes. Conclusion: The beneficial effects of pitavastatin are likely attributable, at least in part, to attenuation of the mineralocorticoid receptor-dependent inflammatory mediator, matrix protein, and cathepsin expressions induced by AT1R-mediated NADPH oxidase activation in the kidneys of a salt-induced hypertensive Dahl salt-sensitive rat model. © 2011 Wolters Kluwer Health | Lippincott Williams & Wilkins.

DOI： 10.1097/HJH.0b013e328341cedf

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Statin Inhibits Apoptosis of Vascular Smooth Muscle Cells in Atherogenic Plaque via eNOs/HIF-1 alpha-Mediated IAP-2 Activation: Implication for Plaque Stability

Cheng Xian Wu, Song Haizhen, Hu Lina, Inoue Aiko, Kuzuya Masfumi, Nakamura Kae, Kim Weon, Shi Guo-Ping, Okumura Kenji, Murohara Toyoaki

CIRCULATION 122 巻 ( 21 ) 2010年11月

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記述言語：日本語

Web of Science
Superoxide-Dependent Cathepsin Activation is Associated with Hypertensive Renal Remodeling and Dysfunction and Represents a Target for Angiotensin Type 1 Receptor Blocker

Cheng Xian Wu, Sasaki Takeshi, Song Haizhen, Inoue Aiko, Fan Janglin, Hirashiki Akihiro, Shi Guo-Ping, Okumura Kenji, Kuzuya Masafumi, Murohara Toyoaki

CIRCULATION 122 巻 ( 21 ) 2010年11月

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記述言語：日本語

Web of Science
Matrix metalloproteinase-2 regulates the expression of tissue inhibitor of matrix metalloproteinase-2

Kimura Kaoru, Cheng Xian Wu, Nakamura Kae, Inoue Aiko, Hu Lina, Song Haizhen, Okumura Kenji, Iguchi Akihisa, Murohara Toyoaki, Kuzuya Masafumi

CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY 37 巻 ( 11 ) 頁： 1096 - 1101 2010年11月

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記述言語：日本語出版者・発行元：Clinical and Experimental Pharmacology and Physiology

1. Matrix metalloproteinases (MMP) are associated with the vascular remodelling seen in atherosclerosis and aneurysm. The activation and activity of MMP-2 are regulated by the intrinsic tissue inhibitor of MMP-2 (TIMP-2). The aim of the present study was to examine whether, conversely, MMP-2 can affect the gene and protein expression of TIMP-2. 2. In the present study, we examined the mRNA and protein expression of MMP-2 and TIMP-2 in cultured smooth muscle cells (SMC) from the aortas of MMP-2+/+ and MMP-2 / mice. We also examined the roles of MMP-2 in SMC cellular events. 3. Western blotting showed that less TIMP-2 protein was present in the conditioned medium of MMP-2 / SMC than in that of MMP-2+/+ SMC. Real-time reverse transcription polymerase chain reaction analysis showed that MMP-2 deficiency reduced TIMP-2 mRNA expression in SMC. Recombinant MMP-2 enhanced the expression of TIMP-2 protein in cultured SMC from MMP-2 / mice. Furthermore, a siRNA targeting MMP-2 impaired the gene and protein expression of MMP-2 in cultured SMC from MMP-2+/+ mice. MMP-2 deficiency impaired SMC invasion, but not their proliferation, adhesion or migration. 4. Our findings suggest that MMP-2 is likely to be responsible, at least in part, for regulating TIMP-2 expression and is thus a potential target, in addition to TIMP-2, for therapeutics aimed at preventing cardiovascular remodelling in response to injury. © 2010 The Authors. Clinical and Experimental Pharmacology and Physiology © 2010 Blackwell Publishing Asia Pty Ltd.

DOI： 10.1111/j.1440-1681.2010.05441.x

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Statin-Induced Prevention of Hypertensive Renal Insufficiency Through Reduction of Local Angiotensin Synthesis and NADPH Oxidase-Dependent Activation of the Mineralocorticoid Receptor

Cheng Xian Wu, Okumura Kenji, Inoue Aiko, Hu Lina, Song Haizhen, Sasaki Takeshi, Kuzuya Masafumi, Murohara Toyoaki

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 30 巻 ( 11 ) 頁： E232 - E232 2010年11月

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記述言語：日本語

Web of Science
Mechanisms Underlying the Exercise Training-Induced Improvement of Neovascularization in Response to Ischemia in Advanced Age

Cheng Xian Wu, Okumura Kenji, Hu Lina, Song Haizhen, Inoue Aiko, Kuzuya Masafumi, Murohara Toyoaki

ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY 30 巻 ( 11 ) 頁： E247 - E247 2010年11月

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記述言語：日本語

Web of Science
Exercise Training Stimulates Ischemia-Induced Neovascularization via Phosphatidylinositol 3-Kinase/Akt-Dependent Hypoxia-Induced Factor-1 alpha Reactivation in Mice of Advanced Age

Cheng Xian Wu, Kuzuya Masafumi, Kim Weon, Song Haizhen, Hu Lina, Inoue Aiko, Nakamura Kae, Di Qun, Sasaki Takeshi, Tsuzuki Michitaka, Shi Guo-Ping, Okumura Kenji, Murohara Toyoaki

CIRCULATION 122 巻 ( 7 ) 頁： 707 - 716 2010年8月

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記述言語：日本語出版者・発行元：Circulation

Background: Exercise stimulates the vascular response in pathological conditions, including ischemia; however, the molecular mechanisms by which exercise improves the impaired hypoxia-induced factor (HIF)-1α-mediated response to hypoxia associated with aging are poorly understood. Here, we report that swimming training (ST) modulates the vascular response to ischemia in aged (24-month-old) mice. Methods and Results: Aged wild-type mice (MMP-2 +/+) that maintained ST (swimming 1 h/d) from day 1 after surgery were randomly assigned to 4 groups that were treated with either vehicle, LY294002, or deferoxamine for 14 days. Mice that were maintained in a sedentary condition served as controls. ST increased blood flow, capillary density, and levels of p-Akt, HIF-1α, vascular endothelial growth factor, Fit-1, and matrix metalloproteinase-2 (MMP-2+/+) in MMP-2 mice. ST also increased the numbers of circulating endothelial progenitor cells and their function associated with activation of HIF-1α. All of these effects were diminished by LY294002, an inhibitor of phosphatidylinositol 3-kinase; enhanced by deferoxamine, an HIF-1α stabilizer; and impaired by knockout of MMP-2. Finally, bone marrow transplantation confirmed that ST enhanced endothelial progenitor cell homing to ischemic sites in aged mice. Conclusions-: ST can improve neovascularization in response to hypoxia via a phosphatidylinositol 3-kinase-dependent mechanism that is mediated by the HIF-1α/vascular endothelial growth factor/MMP-2 pathway in advanced age. © 2010 American Heart Association, Inc.

DOI： 10.1161/CIRCULATIONAHA.109.909218

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Exercise Training Stimulates Neovascularization in Response to Ischemia via HIF-1 alpha-VEGF-mediated Activation of MMP-2 in Advanced Age

Cheng Xian Wu, Kuzuya Masafumi, Hu Lina, Kim Weon, Song Haizhen, Inoue Aiko, Shi Guo-Ping, Okumura Kenji, Murohara Toyoaki

CIRCULATION 120 巻 ( 18 ) 頁： S532 - S532 2009年11月

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記述言語：日本語

Web of Science
TREATMENT OF APO E-DEFICIENT MICE WITH STATIN INHIBITS THE OXIDATIVE STRESS-DEPENDENT LYSOSMAL PROTEASE CATHEPSIN ACTIVATION SYSTEM: IMPLICATION FOR PLAQUE STABILITY

Cheng X. W., Kuuzya M., Sasaki T., Nakamura K., Song H., Hu L., Inoue A., Shi G-P, Murohara T., Okumura K.

ATHEROSCLEROSIS SUPPLEMENTS 10 巻 ( 2 ) 2009年6月

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記述言語：日本語

Web of Science
Treatment of ApoE-Deficient Mice with Statin Inhibits the Oxidative Stress-Dependent Lysosomal Protease Cathepsin Activation System: Implication for Plaque Stability

Cheng Xian Wu, Kuzuya Masafumi, Okumura Kenjl, Nakamura Kae, Inoue Aiko, Sasaki Takeshi, Song Haizhen, Takeshita Kyosuke, Kim Weon, Shi Guo-Ping, Murohara Toyoaki

CIRCULATION 118 巻 ( 18 ) 頁： S559 - S559 2008年10月

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記述言語：日本語

Web of Science
Angiogenic activity of bFGF and VEGF suppressed by proteolytic cleavage by neutrophil elastase

Ai Shingo, Cheng Xian Wu, Inoue Aiko, Nakamura Kae, Okumura Kenji, Iguchi Akihisa, Murohara Toyoaki, Kuzuya Masafumi

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS 364 巻 ( 2 ) 頁： 395 - 401 2007年12月

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記述言語：日本語出版者・発行元：Biochemical and Biophysical Research Communications

Neutrophil elastase (NE), a serine protease released from the azurophil granules of activated neutrophil, proteolytically cleaves multiple cytokines, and cell surface proteins. In the present study, we examined whether NE affects the biological abilities of angiogenic growth factors such as basic-fibroblast growth factor (bFGF) and vascular endothelial growth factor (VEGF). NE degraded bFGF and VEGF in a time- and concentration-dependent manner, and these degradations were suppressed by sivelestat, a synthetic inhibitor of NE. The bFGF- or VEGF-mediated proliferative activity of human umbilical vein endothelial cells was inhibited by NE, and the activity was recovered by sivelestat. Furthermore, NE reduced the bFGF- or VEGF-induced tubulogenic response of the mice aortas, ex vivo angiogenesis assay, and these effects were also recovered by sivelestat. Neutrophil-derived NE degraded potent angiogenic factors, resulting in loss of their angiogenic activity. These findings provide additional insight into the role played by neutrophils in the angiogenesis process at sites of inflammation. © 2007 Elsevier Inc. All rights reserved.

DOI： 10.1016/j.bbrc.2007.10.027

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Azelnidipine enhances beneficial effects of olmesartan on left ventricular remodeling during the development of hypertension-induced heart failure

Cheng Xian Wu, Okumura Kenji, Nagata Kohzo, Inoue Aiko, Zhang Jie, Nishizawa Takao, Yamada Takashi, Murase Yosuke, Izawa Hideo, Asano Hiroyuki, Obata Koji, Kuzuya Masafumi, Shi Guo-Ping, Murohara Toyoaki, Yokota Mitsuhiro

CIRCULATION 116 巻 ( 16 ) 頁： 557 - 557 2007年10月

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記述言語：日本語

Web of Science
Superoxide-dependent cathepsin activation system is associated with hypertensive myocardial remodeling and represents a target for angiotesin II type 1 receptor blocker therapy

Cheng Xian Wu, Okumura Kenji, Nagata Kohzo, Izawa Hideo, Obata Koji, Sasaki Takeshi, Inoue Aiko, Nishizawa Takao, Yamada Takashi, Kobayashi Masakazu, Inden Yasuya, Murase Yosuke, Kuzuya Mafumi, Shi Guo-Ping, Murohara Toyoaki, Yokota Mitsuhiro

CIRCULATION 116 巻 ( 16 ) 頁： 742 - 742 2007年10月

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記述言語：日本語

Web of Science
Mechanisms underlying the impairment of ischemia-induced Neovascularization in aging mice: the pivotal role for matirx-metalloproteinase-2

Cheng Man Wu, Kuzuya Masafumi, Okumura Kenji, Inoue Aiko, Kim Weon, Yokota Mitsuhiro, Murohara Toyoaki

CIRCULATION 116 巻 ( 16 ) 頁： 24 - 25 2007年10月

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記述言語：日本語

Web of Science

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論文の先頭へ▲

書籍等出版物 1

最先端ナノライフシステム研究

（担当：分担執筆 , 範囲: 4章　健康寿命の延伸に関する実装研究）

丸善プラネット株式会社 2022年3月（ ISBN:978-4-86345-520-7 ）

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総ページ数：215 担当ページ：63-69 記述言語：日本語著書種別：教科書・概説・概論

書籍等出版物の先頭へ▲

MISC 4

(特集老化・フレイルとサルコペニア) フレイル・サルコペニアとバイオマーカー

井上愛子、葛谷雅文、成憲武

CLINICAL CALCIUM28 巻 ( 9 ) 頁： 1191 - 1200 2018年8月

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担当区分：筆頭著者記述言語：日本語
(老年医学の展望) サルコペニアの分子メカニズム

井上愛子、成憲武、五籐大貴、葛谷雅文

日本老年医学会雑誌55 巻頁： 13 - 24 2018年

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DOI： 10.3143/geriatrics.55.13
冠動脈硬化の新しい分子機序:炎症•免疫からみた最新の知見

成憲武、井上愛子

医学の歩み259 巻 ( 6 ) 頁： 590 - 596 2016年
(特集高齢者医療におけるサルコペニア・フレイル対策) サルコペニア・フレイルのバイオマーカー

井上愛子、成憲武、葛谷雅文

医薬ジャーナル51 巻 ( 9 ) 頁： 67 - 71 2015年9月

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担当区分：筆頭著者

DOI： https://doi.org/10.20837/1201509051

MISCの先頭へ▲

講演・口頭発表等 14

Cathepsin K activity controls injury-related vascular repair in mice

Hu L., Cheng X.W., Song H., Inoue A., Jiang H., Li X., Shi G.P., Kozawa E., Okumura K., Kuzuya M.

Hypertension 2014年3月 Hypertension

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記述言語：日本語

Cathepsin K (CatK) is one of the most potent mammalian collagenases. We showed previously the increased expression of CatK in human and animal atherosclerotic lesions. Here, we hypothesized that ablation of CatK mitigates injury-induced neointimal hyperplasia. Male wild-type (CatK) and CatK-deficient (CatK) mice underwent ligation or a combination of ligation and polyethylene cuff-replacement injuries to the right common carotid artery just proximal to its bifurcation, and they were then processed for morphological and biochemical studies at specific time points. On operative day 28, CatK significantly reduced neointimal formation and neovessel formation in both single- and combination-injured arteries compared with the Cat K mice. At early time points, CatK reduced the lesion macrophage contents and medial smooth muscle cell proliferation, the mRNA levels of monocyte chemoattractant protein-1, toll-like receptor-2, toll-like receptor-4, chemokine ligand-12, and the gelatinolytic activity related to matrix metalloproteinase-2/-9. An aorta-explant assay revealed that smooth muscle cell movement was impaired in the CatK mice compared with the CatK mice. In addition, the smooth muscle cells and macrophages from CatK mice had less invasive ability through a reconstituted basement membrane barrier. This vasculoprotective effect was mimicked by Cat inhibition with trans-epoxysuccinyl-L-leucylamido-{4-guanidino} butane (E64d). These results demonstrate an essential role of CatK in neointimal lesion formation in response to injury, possibly via the reduction of toll-like receptor-2/-4-mediated inflammation and smooth muscle cell proliferation, suggesting a novel therapeutic strategy for the control of endovascular treatment-related restenosis by regulating CatK activity. © 2013 American Heart Association, Inc.

DOI： 10.1161/HYPERTENSIONAHA.113.02141

Scopus

PubMed
Mechanism of diastolic stiffening of the failing myocardium and its prevention by angiotensin receptor and calcium channel blockers

Cheng X.W., Okumura K., Kuzuya M., Jin Z., Nagata K., Obata K., Inoue A., Hirashiki A., Takeshita K., Unno K., Harada K., Shi G.P., Yokota M., Murohara T.

Journal of Cardiovascular Pharmacology 2009年7月 Journal of Cardiovascular Pharmacology

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記述言語：日本語

OBJECTIVE: To investigate the mechanism responsible for the increased cardiac stiffness associated with hypertensive heart failure in Dahl salt-sensitive (DS) rats and the effects of treatment with the combination of a calcium channel blocker [azelnidipine (AZE)] and angiotensin II type 1 receptor blocker [olmesartan (OLM)]. METHODS: DS rats fed a high-salt diet from 7 weeks of age were treated (or not) from 12 to 19 weeks of age with the vasodilator hydralazine, OLM plus AZE, or the reduced nicotinamide adenine dinucleotide phosphate (NADPH) oxidase inhibitor apocynin. Rats fed a low-salt diet served as controls. RESULTS: Treatment with OLM plus AZE attenuated changes in the expression of collagen isoforms and a decrease in the ratio of elastin to collagen in the left ventricle and prevented the increase in myocardial stiffness and diastolic dysfunction in DS rats in a manner independent of the hypotensive effect of these drugs. Such treatment also inhibited the expression and activation of elastolytic proteases (including cathepsins S and K and metalloproteinases-2, -9, and -12), NADPH oxidase-dependent superoxide production, and inflammatory changes in the failing myocardium. All these effects were mimicked by treatment with apocynin. CONCLUSIONS: The changes in collagen isoform expression and the decrease in the elastin to collagen ratio in the failing myocardium likely account for the increase in diastolic stiffness in this model of hypertensive heart failure. Administration of angiotensin receptor and calcium channel blockers prevented these changes in a manner independent of the hypotensive effect of these drugs by inhibiting the increase in elastolytic activity induced by activation of NADPH oxidase. © 2009 Lippincott Williams & Wilkins, Inc.

DOI： 10.1097/FJC.0b013e3181ab371d

Scopus

PubMed
食事摂取と体位が健常者の核心温ならびに血圧に及ぼす影響 : 炭水化物摂取と仰臥位・長坐位における検討

長谷川愛子, 伊藤剛, 石津みゑ子, 花輪壽彦

自律神経 = The Autonomic nervous system 2005年6月15日

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記述言語：日本語

CiNii Research
Mechanisms underlying the impairment of ischemia-induced neovascularization in MMP-2-deficient mice

Circulation journal : official journal of the Japanese Circulation Society 2007年10月20日 Japanese Circulation Society

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記述言語：英語

CiNii Research
Mechanisms underlying the impairment of ischemia-induced neovascularization in MMP-2-deficient mice

Cheng Xian-Wu, Kuzuya Masafumi, Nakamura Kae, Inoue Aiko, Hirata Makiko, Tsuzuki Michitaka, Kondo Takahisa, Kim Woen, Okumura Kenji, Yokota Mitsuhiro, Murohara Toyoaki, Iguchi Akihisa

Circulation journal : official journal of the Japanese Circulation Society 2007年3月1日 Japanese Circulation Society

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記述言語：英語

CiNii Research
裏寒と手足厥寒における四逆湯の温熱生理学的検討(EBM, 第58回日本東洋医学会学術総会)

伊藤剛, 井上愛子, 若杉安希乃, 及川哲郎, 花輪壽彦

第58回日本東洋医学会学術総会 2007年5月社団法人日本東洋医学会

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記述言語：日本語

CiNii Research
看護学科学生の東洋医学に対する意識調査

長谷川愛子, 伊藤剛, 石野尚吾, 花輪壽彦

日本東洋醫學雜誌 2001年5月1日社団法人日本東洋医学会

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記述言語：日本語

CiNii Research
温熱生理学的にみた漢方薬の生理作用 : 当帰四逆加呉茱萸生姜湯について

伊藤剛, 井上愛子, 若杉安希乃, 及川哲郎, 花輪壽彦

第57回日本東洋医学会学術総会 2006年5月31日社団法人日本東洋医学会

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記述言語：日本語

CiNii Research
サーモグラフィと尿温による冷え症のパターンと裏寒の温熱生理学的検討

伊藤剛, 井上愛子, 若杉安希乃, 及川哲郎, 花輪壽彦

第59回日本東洋医学会学術総会 2008年5月7日社団法人日本東洋医学会

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記述言語：日本語

CiNii Research
中核温からみた「冷え症」の病態解析(04病態(現代医学)(1))

伊藤剛, 長谷川愛子, 高橋裕子, 花輪壽彦

日本東洋醫學雜誌 2003年4月社団法人日本東洋医学会

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記述言語：日本語

CiNii Research
中核温ならびに自律神経機能よりみた「四肢型冷え症」の病態(03 病態(現代医学)(1))

伊藤剛, 長谷川愛子, 高橋裕子, 花輪壽彦

日本東洋醫學雜誌 2004年5月社団法人日本東洋医学会

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記述言語：日本語

CiNii Research
「食後佳眠倦怠」の病態解析(第1報) : 健常者における食後傾眠について(02病態(伝統医学)(1))

長谷川愛子, 伊藤剛, 花輪壽彦

日本東洋醫學雜誌 2003年4月社団法人日本東洋医学会

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記述言語：日本語

CiNii Research
Mechanisms Underlying the Impairment of Ischemia-induced Neovascularization in Aging Mice : the Pivotal Role for Matirx-Metalloproteinase-2(Regeneration(angiogenesis/myocardial regeneration)

Cheng Xian Wu, Kuzuya Masafumi, Okumura Kenji, Inoue Aiko, Nakamura Kae, Sasaki Takeshi, Kim Veon, Yokota Mitsuhiro, Murohara Toyoaki

The 72nd Annual Scientific Meeting of the Japanese Circulation Society 2008年3月1日 Japanese Circulation Society

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記述言語：英語

CiNii Research
Treatment of ApoE-Deficient Mice with Statin Inhibits the Oxidative Stress-dependent Lysosomal Protease Cathepsin Activation System : Implication for Plaque Stability

Cheng Xian Wu, Kuzuya Masafumi, Nakamura Kae, Okumura Kenji, Inoue Aiko, Sasaki Takeshi, Yokota Mitsuhiro, Murohara Toyoaki

The 72nd Annual Scientific Meeting of the Japanese Circulation Society 2008年3月1日 Japanese Circulation Society

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記述言語：英語

CiNii Research

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講演・口頭発表等の先頭へ▲

科研費 3

独居高齢者に対するICTを活用した包括的フレイル予防システムの運用による介入効果

研究課題/研究課題番号：21K17436 2021年4月 - 2024年3月

科学研究費助成事業若手研究

井上愛子

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担当区分：研究代表者

配分額：4680000円（直接経費：3600000円、間接経費：1080000円）

世界に類を見ないスピードで超高齢社会を迎えている我が国において、ケアが必要な高齢者の絶対数の増加と現役世代の減少により医療・介護を担う人的資源が枯渇していく中で、持続可能なフレイルへの対策が急務となっている。
本研究では、申請者らが2019年度に構築した循環型健康寿命延伸プログラムをスマートスピーカーを活用した独居高齢者向けのフレイル予防アプリケーション（独居高齢者見守りシステム）として新たに改良し、包括的フレイル予防支援システムによる独居高齢者の心身機能の維持・向上やフレイル予防効果ならびに健康行動促進要因を明らかにする。
超高齢社会における健康増進のためのICT/IoT利活用に関する研究

研究課題/研究課題番号：21K12593 2021年4月 - 2024年3月

科学研究費助成事業基盤研究(C)

浦田真由, 安田孝美, 井上愛子

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担当区分：研究分担者

超高齢社会において，高齢者の社会的孤立をいかに防ぐかは自治体の大きな課題となっているが，新型コロナウイルス感染症の影響により，地域での高齢者支援サービスの実施が困難となっている。本研究では，地域在住高齢者の健康増進のためのICT/IoT利活用を社会情報学と老年医学の学際研究として取り組み，地域への実装によってその効果を検証する。健康寿命を延ばすためのモデルを構築し，地域のICT/IoT 利活用を支える担い手の育成を行うことで，研究期間後も取り組みを継続させることを目指している。
フレイルおよびサルコペニア予防のための骨格筋リモデリングと再生機構解明

研究課題/研究課題番号：18K15414 2018年4月 - 2021年3月

科学研究費助成事業若手研究

井上愛子

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担当区分：研究代表者

配分額：4290000円（直接経費：3300000円、間接経費：990000円）

高齢期の骨格筋は障害を伴っていることが多く（サルコペニア、フレイルなど）、治療には骨格筋再生へのアプローチが必要である。細胞の分化・増殖、及びアポトーシスにおいて重要な役割を果たし、老化に伴いそのシグナル伝達の不活化が起こることが報告されているNotch シグナルとその活性化に関与するカテプシンKに着目し、サルコペニア形成、骨格筋のリモデリングや再生への関与を検討した。カテプシンKは、サルコペニアを代表とする骨格筋障害に関与することを明らかにした。
カテプシンKが、カヘキシアモデルマウスの骨格筋の萎縮と機能障害にも関与している可能性が示唆されたことにより、今後は高齢者のQOLやADLの低下予防や、フレイルなどの改善のための新たな分子標的になる可能性が示唆された。

科研費の先頭へ▲