Organization
Institute of Transformative Bio-Molecules Designated associate professor
Graduate School
Graduate School of Science
Title
Designated associate professor
NAKAMURA Masayoshi
Degree 1
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Ph.D. (Bioscience) （ 2009.3 Nara Institute of Science and Technology ）
Research Interests 1
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Cell biology, Live cell imaging, Cytoskeleton, Microtubule, SWEET transporter
Research Areas 1
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Life Science / Plant molecular biology and physiology / Cell biology, Live cell imaging, Cytoskeleton, Microtubule, SWEET transporter
Current Research Project and SDGs 3
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膜輸送タンパク質の基質選択性の解析と阻害剤の単離
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代謝物の生体内動態を可視化する蛍光プローブと代謝制御ツールの開発
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Cytoskeletal organization in response to environmental stimulus
Research History 4
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Nagoya University Institute of Transformative Bio-Molecules Designated associate professor
2021.11
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Nagoya University Institute of Transformative Bio-Molecules Designated lecturer
2017.4 - 2021.11
Country：Japan
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Carnegie Institution for Science Department of Plant Biology Researcher
2011.4 - 2017.3
Country：United States
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Nara Institute of Science and Technology Researcher
2009.4 - 2011.3
Country：Japan
Education 2
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Nara Institute of Science and Technology
- 2009.3
Country： Japan
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Kyoto University School of Agriculture
- 2003.3
Country： Japan
Professional Memberships 2
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日本分子生物学会
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日本植物生理学会
Awards 2
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Career Development Award
2017 Human Frontier Science Program
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Tanslay Medal Finalist
2014 New Phytologist Trust
Papers 25
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Plant microtubule nucleating apparatus and its potential signaling pathway Invited Reviewed International coauthorship
Noriyoshi Yagi, Satoshi Fujita, Masayoshi Nakamura
Current Opinion in Plant Biology Vol. 82 2024.9
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Near-infrared imaging of phytochrome-derived autofluorescence in plant nuclei Reviewed International coauthorship
Yoshinari, A; Isoda, R; Yagi, N; Sato, Y; Lindeboom, JJ; Ehrhardt, DW; Frommer, WB; Nakamura, M
PLANT JOURNAL 2024.3
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SWEET13 transporter of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14 Reviewed International coauthorship
Reika Isoda, Zoltan Palmai, Akira Yoshinari, Li-Qing Chen, Florence Tama, Wolf B. Frommer, Masayoshi Nakamura
Proceedings of the National Academy of Sciences of the United States of America ( 119 ) page： e2207558119 2022.10
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OsSWEET11b, a potential sixth leaf blight susceptibility gene involved in sugar transport-dependent male fertility Reviewed International coauthorship
Wu Lin-Bo, Eom Joon-Seob, Isoda Reika, Li Chenhao, Char Si Nian, Luo Dangping, Schepler-Luu Van, Nakamura Masayoshi, Yang Bing, Frommer Wolf B.
NEW PHYTOLOGIST Vol. 234 ( 3 ) page： 975 - 989 2022.5
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Finding a right place to cut: how katanin is targeted to cellular severing sites Invited Reviewed
Masayoshi Nakamura, Noriyoshi Yagi, Takashi Hashimoto
Quantitative Plant Biology ( 3 ) page： e8 2022.4
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Designs, applications and limitations of genetically encoded fluorescent sensors to explore plant biology – looking back and ahead Reviewed International coauthorship
Mayuri Sadoine, Yuuma Ishikawa, Thomas J. Kleist, Michael M. Wudick, Masayoshi Nakamura, Guido Grossmann, Wolf B. Frommer and Cheng-Hsun Ho
PLANT PHYSIOLOGY 2021.8
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Advances in synthetic fluorescent probe labeling for live cell imaging in plants. Reviewed International coauthorship
Noriyoshi Yagi, Akira Yoshinari, Ryu J Iwatate, Reika Isoda, Wolf B Frommer, Masayoshi Nakamura
PLANT AND CELL PHYSIOLOGY 2021.7
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An anchoring complex recruits katanin for microtubule severing at the plant cortical nucleation sitesAn anchoring Reviewed International coauthorship
Noriyoshi Yagi, Takehide Kato, Sachihiro Matsunaga, David W Ehrhardt, Masayoshi Nakamura, Takashi Hashimoto
NATURE COMMUNICATIONS ( 12 ) page： 3687 2021.6
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Sensors for the quantification, localization and analysis of the dynamics of plant hormones Reviewed International coauthorship
Isoda Reika, Yoshinari Akira, Ishikawa Yuuma, Sadoine Mayuri, Simon Rudiger, Frommer Wolf B., Nakamura Masayoshi
PLANT JOURNAL Vol. 105 ( 2 ) page： 542 - 557 2021.1
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Using Genetically Encoded Fluorescent Biosensors for Quantitative In Vivo Imaging Reviewed International coauthorship
Yoshinari Akira, Moe-Lange Jacob, Kleist Thomas J., Cartwright Heather N., Quint David A., Ehrhardt David W., Frommer Wolf B., Nakamura Masayoshi
ARABIDOPSIS PROTOCOLS, 4 EDITION Vol. 2200 page： 303 - 322 2021
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Mechanistic Insights into Plant Chiral Growth Reviewed
Nakamura Masayoshi, Hashimoto Takashi
SYMMETRY-BASEL Vol. 12 ( 12 ) 2020.12
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Covalent Self-Labeling of Tagged Proteins with Chemical Fluorescent Dyes in BY-2 Cells and Arabidopsis Seedlings([OPEN]) Reviewed International coauthorship
Iwatate Ryu J., Yoshinari Akira, Yagi Noriyoshi, Grzybowski Marek, Ogasawara Hiroaki, Kamiya Mako, Komatsu Toru, Taki Masayasu, Yamaguchi Shigehiro, Frommer Wolf B., Nakamura Masayoshi
PLANT CELL Vol. 32 ( 10 ) page： 3081 - 3094 2020.10
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The sucrose transporter MdSUT4.1 participates in the regulation of fruit sugar accumulation in apple Reviewed International coauthorship
Peng Qian, Cai Yaming, Lai Enhui, Nakamura Masayoshi, Liao Liao, Zheng Beibei, Ogutu Collins, Cherono Sylvia, Han Yuepeng
BMC PLANT BIOLOGY Vol. 20 ( 1 ) 2020.5
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A Novel Katanin-Tethering Machinery Accelerates Cytokinesis Reviewed
Sasaki Takema, Tsutsumi Motosuke, Otomo Kohei, Murata Takashi, Yagi Noriyoshi, Nakamura Masayoshi, Nemoto Tomomi, Hasebe Mitsuyasu, Oda Yoshihisa
CURRENT BIOLOGY Vol. 29 ( 23 ) page： 4060 - + 2019.12
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CLASP stabilization of plus ends created by severing promotes microtubule creation and reorientation Reviewed International coauthorship
Lindeboom Jelmer J., Nakamura Masayoshi, Saltini Marco, Hibbel Anneke, Walia Ankit, Ketelaar Tijs, Emons Anne Mie C., Sedbrook John C., Kirik Viktor, Mulder Bela M., Ehrhardt David W.
JOURNAL OF CELL BIOLOGY Vol. 218 ( 1 ) page： 190 - 205 2019.1
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SPR2 protects minus ends to promote severing and reorientation of plant cortical microtubule arrays Reviewed International coauthorship
Nakamura Masayoshi, Lindeboom Jelmer J., Saltini Marco, Mulder Bela M., Ehrhardt David W.
JOURNAL OF CELL BIOLOGY Vol. 217 ( 3 ) page： 915 - 927 2018.3
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Microtubule nucleating and severing enzymes for modifying microtubule array organization and cell morphogenesis in response to environmental cues Reviewed
Masayoshi Nakamura
New Phytologist 2015.2
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GCP-WD Mediates gamma-TuRC Recruitment and the Geometry of Microtubule Nucleation in Interphase Arrays of Arabidopsis Reviewed International coauthorship
Ankit Walia , Masayoshi Nakamura , Dorianne Moss , Viktor Kirik , Takashi Hashimoto , David W. Ehrhardt
CURRENT BIOLOGY Vol. 24 ( 21 ) 2014.11
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A Mechanism for Reorientation of Cortical Microtubule Arrays Driven by Microtubule Severing Reviewed International coauthorship
Jelmer J. Lindeboom , Masayoshi Nakamura , Anneke Hibbel , Kostya Shundyak , Ryan Gutierrez , Tijs Ketelaar , Anne Mie C. Emons , Bela M. Mulder , Viktor Kirik , David W. Ehrhardt
SCIENCE Vol. 342 ( 6163 ) 2013.12
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Arabidopsis GCP3-interacting protein 1/MOZART 1 is an integral component of the gamma-tubulin-containing microtubule nucleating complex Reviewed International coauthorship
Masayoshi Nakamura , Noriyoshi Yagi , Takehide Kato , Satoshi Fujita , Noriyuki Kawashima , David W. Ehrhardt , Takashi Hashimoto
PLANT JOURNAL Vol. 71 ( 2 ) 2012.7
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RNA Processing Bodies, Peroxisomes, Golgi Bodies, Mitochondria, and Endoplasmic Reticulum Tubule Junctions Frequently Pause at Cortical Microtubules Reviewed International coauthorship
Takahiro Hamada , Motoki Tominaga , Takashi Fukaya , Masayoshi Nakamura , Akihiko Nakano , Yuichiro Watanabe , Takashi Hashimoto , Tobias I. Baskin
PLANT AND CELL PHYSIOLOGY Vol. 53 ( 4 ) 2012.4
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A plastidial sodium-dependent pyruvate transporter Reviewed International coauthorship
Tsuyoshi Furumoto , Teppei Yamaguchi , Yumiko Ohshima-Ichie , Masayoshi Nakamura , Yoshiko Tsuchida-Iwata , Masaki Shimamura , Junichi Ohnishi , Shingo Hata , Udo Gowik , Peter Westhoff , Andrea Braeutigam , Andreas P. M. Weber , Katsura Izui
NATURE Vol. 476 ( 7361 ) 2011.8
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Microtubule and katanin-dependent dynamics of microtubule nucleation complexes in the acentrosomal Arabidopsis cortical array Reviewed International coauthorship
Masayoshi Nakamura , David W. Ehrhardt , Takashi Hashimoto
NATURE CELL BIOLOGY Vol. 12 ( 11 ) 2010.11
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A mutation in the Arabidopsis gamma-tubulin-containing complex causes helical growth and abnormal microtubule branching Reviewed
JOURNAL OF CELL SCIENCE Vol. 122 ( 13 ) 2009.7
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Low concentrations of propyzamide and oryzalin alter microtubule dynamics in Arabidopsis epidermal cells Reviewed
M Nakamura , K Naoi , T Shoji , T Hashimoto
PLANT AND CELL PHYSIOLOGY Vol. 45 ( 9 ) 2004.9
KAKENHI (Grants-in-Aid for Scientific Research) 4
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Grant number：23K27166 2024.2 - 2026.3
科学研究費助成事業 基盤研究(B)
中村 匡良
Authorship：Principal investigator
Grant amount：\12090000 （ Direct Cost: \9300000 、 Indirect Cost：\2790000 ）
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Grant number：23H02473 2023.4 - 2026.3
科学研究費助成事業 基盤研究(B)
中村 匡良
Authorship：Principal investigator
Grant amount：\18720000 （ Direct Cost: \14400000 、 Indirect Cost：\4320000 ）
遺伝学的解析により、微小管重合因子の一アミノ酸置換の変異が非つる性シロイヌナズナ植物の細胞に左巻きの微小管配置をもたらし、右巻きのねじれ伸長を根や茎などの軸組織に引き起こすことを明らかにした。しかし、この変異がどのように左巻きの微小管配置をもたらすかは理解されていない。本研究では、微小管重合因子の一アミノ酸置換がなぜ植物に右巻きねじれを引き起こすかを明らかにし、微小管重合因子複合体からの微小管形成の分子基盤の解明することで、微小管形成機構の生理的・形態的な役割を明らかにすることを目指す。
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Grant number：20K21424 2020.7 - 2022.3
科学研究費助成事業 挑戦的研究(萌芽)
中村 匡良
Authorship：Principal investigator
Grant amount：\6500000 （ Direct Cost: \5000000 、 Indirect Cost：\1500000 ）
近年の研究からγチューブリン複合体とカタニンがそれぞれ細胞分裂にも重要であり、細胞分裂中の各微小管構造体に局在することが報告されている。局在は確認され重要性は理解できるものの、紡錘体や隔膜形成体の形成過程や各構造体への移り変わりに微小管形成と微小管切断がどのように関わっているかは研究されていない。研究が困難な理由は、それぞれの構造体や移り変わりでのみタンパク質を不活化できないからである。そこで本研究では、γチューブリン複合体とカタニンの時間的空間的な機能を理解することを目的とし、ライブイメージング中のタンパク質機能阻害技術の確立に挑戦する。
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Mechanical manipulation and in vitro reconstitution of microtubule severing
Grant number：18KK0195 2018.10 - 2022.3
Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
Authorship：Principal investigator
Grant amount：\17940000 （ Direct Cost: \13800000 、 Indirect Cost：\4140000 ）