Updated on 2024/04/19

写真a

 
SUGIURA Daisuke
 
Organization
Graduate School of Bioagricultural Sciences Department of Plant Production Sciences Lecturer
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Bioresource Sciences
Title
Lecturer
External link

Degree 1

  1. 博士(理学) ( 2012.3   東京大学 ) 

Research Areas 3

  1. Life Science / Ecology and environment

  2. Environmental Science/Agriculture Science / Crop production science

  3. Life Science / Ecology and environment

Professional Memberships 3

  1. The Ecological Society of Japan

  2. Crop Science Society of Japan

  3. The Botanical Society of Japan

 

Papers 40

  1. Control of root nodule formation ensures sufficient shoot water availability in <i>Lotus japonicus</i> Reviewed

    Kensuke Kawade, Daisuke Sugiura, Akira Oikawa, Masayoshi Kawaguchi

    Plant Physiology     2024.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Oxford University Press (OUP)  

    Abstract

    Leguminous plants provide carbon to symbiotic rhizobia in root nodules to fuel the energy-consuming process of nitrogen fixation. The carbon investment pattern from the acquired sources is crucial for shaping the growth regime of the host plants. The autoregulation of nodulation (AON) signaling pathway tightly regulates the number of nodules that form. AON disruption leads to excessive nodule formation and stunted shoot growth. However, the physiological role of AON in adjusting the carbon investment pattern is unknown. Here, we show that AON plays an important role in sustaining shoot water availability, which is essential for promoting carbon investment in shoot growth in Lotus japonicus. We found that AON-defective mutants exhibit substantial accumulation of nonstructural carbohydrates, such as sucrose. Consistent with this metabolic signature, resilience against water-deficit stress was enhanced in the shoots of the AON-defective mutants. Furthermore, the water uptake ability was attenuated in the AON-defective mutants, likely due to the increased ratio of nodulation zone, which is covered with hydrophobic surfaces, on the roots. These results increase our physiological understanding of legume–rhizobia symbiosis by revealing a trade-off between root nodule formation and shoot water availability.

    DOI: 10.1093/plphys/kiae126

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  2. Exploring the responses of crop photosynthesis to CO2 elevation at the molecular, physiological, and morphological levels toward increasing crop production Reviewed

    Daisuke Sugiura, Wang Yin, Masaru Kono, yusuke mizokami

    Crop and Environment     2023.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.crope.2023.11.006

  3. Sink–source imbalance triggers delayed photosynthetic induction: Transcriptomic and physiological evidence Reviewed

    Yui Ozawa, Aiko Tanaka, Takamasa Suzuki, Daisuke Sugiura

    Physiologia Plantarum   Vol. 175 ( 5 ) page: e14000   2023.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    <jats:title>Abstract</jats:title><jats:p>Sink–source imbalance causes accumulation of nonstructural carbohydrates (NSCs) and photosynthetic downregulation. However, despite numerous studies, it remains unclear whether NSC accumulation or N deficiency more directly decreases steady‐state maximum photosynthesis and photosynthetic induction, as well as underlying gene expression profiles. We evaluated the relationship between photosynthetic capacity and NSC accumulation induced by cold girdling, sucrose feeding, and low nitrogen treatment in <jats:italic>Glycine max</jats:italic> and <jats:italic>Phaseolus vulgaris</jats:italic>. In <jats:italic>G</jats:italic>. <jats:italic>max</jats:italic>, changes in transcriptome profiles were further investigated, focusing on the physiological processes of photosynthesis and NSC accumulation. NSC accumulation decreased the maximum photosynthetic capacity and delayed photosynthetic induction in both species. In <jats:italic>G</jats:italic>. <jats:italic>max</jats:italic>, such photosynthetic downregulation was explained by coordinated downregulation of photosynthetic genes involved in the Calvin cycle, Rubisco activase, photochemical reactions, and stomatal opening. Furthermore, sink–source imbalance may have triggered a change in the balance of sugar‐phosphate translocators in chloroplast membranes, which may have promoted starch accumulation in chloroplasts. Our findings provide an overall picture of photosynthetic downregulation and NSC accumulation in <jats:italic>G</jats:italic>. <jats:italic>max</jats:italic>, demonstrating that photosynthetic downregulation is triggered by NSC accumulation and cannot be explained solely by N deficiency.</jats:p>

    DOI: 10.1111/ppl.14000

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  4. Tight relationship between two photosystems is robust in rice leaves under various nitrogen conditions Reviewed

    Hiroshi Ozaki, Yusuke Mizokami, Daisuke Sugiura, Takayuki Sohtome, Chikahiro Miyake, Hidemitsu Sakai, Ko Noguchi

    Journal of Plant Research   Vol. 136 ( 2 ) page: 201 - 210   2023.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Science and Business Media LLC  

    DOI: 10.1007/s10265-022-01431-7

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    Other Link: https://link.springer.com/article/10.1007/s10265-022-01431-7/fulltext.html

  5. 3D reconstruction of rice leaf tissue for proper estimation of surface area of mesophyll cells and chloroplasts facing intercellular airspaces from 2D section images. Reviewed International journal

    Rachana Ouk, Takao Oi, Daisuke Sugiura, Mitsutaka Taniguchi

    Annals of botany   Vol. 130 ( 7 ) page: 991 - 998   2022.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    BACKGROUND AND AIMS: The surface area of mesophyll cells (Smes) and chloroplasts (Sc) facing the intercellular airspace (IAS) are important parameters for estimating photosynthetic activity from leaf anatomy. Although the Smes and Sc are estimated based on the shape assumption of mesophyll cells (MC), it is questionable if the assumption is correct for rice MC with concave-convex surfaces. Therefore, in this study, we establish the reconstruction method for the 3D representation of the IAS in rice leaf tissue to calculate the actual Smes and Sc with 3D images and to determine the correct shape assumption for the estimation of Smes and Sc based on 2D section images. METHODS: Here, we used serial section light microscopy (ssLM) to reconstruct the 3D representations of the IAS, MC, and chloroplasts in rice leaf tissue. Actual Smes and Sc values obtained from the 3D representation were compared with those estimated from the 2D images to find out the correct shape-specific assumption (oblate or prolate spheroid) on different orientations (longitudinal and transversal sections) using the same leaf sample. KEY RESULTS: The 3D representation method revealed that volumes of the IAS and MC accounted for 30% and 70% of rice leaf tissue excluding epidermis, respectively, and volume of chloroplasts accounted for 44% of MC. The shape-specific assumption on the sectioning orientation affected the estimation of Smes and Sc using 2D section images with discrepancies of 10‒38%. CONCLUSIONS: The 3D representation of rice leaf tissue was successfully reconstructed using ssLM and suggested that estimation of Smes and Sc of the rice leaf is more accurate using longitudinal sections with MC assumed as oblate spheroid than transversal sections with MC as prolate spheroid.

    DOI: 10.1093/aob/mcac133

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  6. Cost-benefit analysis of mesophyll conductance - Diversities of anatomical, biochemical and environmental determinants. Reviewed International journal

    Yusuke Mizokami, Riichi Oguchi, Daisuke Sugiura, Wataru Yamori, Ko Noguchi, Ichiro Terashima

    Annals of botany   Vol. 130 ( 3 ) page: 265 - 283   2022.9

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    BACKGROUND: Plants invest photosynthates in construction and maintenance of their structures and functions. Such investments are considered costs. These costs are recovered by CO2 assimilation rate (A) in the leaves and thus A is regarded as the immediate, short-term benefit. In photosynthesising leaves, CO2 diffusion from the air to the carboxylation site is hindered by several structural and biochemical barriers. CO2 diffusion from the intercellular air space to the chloroplast stroma is obstructed by the mesophyll resistance. The inverses is the mesophyll conductance (gm). Whether various plants realize an optimal gm, and how much investments are needed for relevant gm, remain unsolved. SCOPE: This review examines relationships among leaf construction costs (CC), leaf maintenance costs (MC) and gm in various plants under diverse growth conditions. Through a literature survey, we demonstrate a strong linear relationship between leaf mass per area (LMA) and leaf CC. The overall correlation of CC versus gm across plant phylogenetic groups is weak, but significant trends are evident within specific groups and/or environments. Investment in CC is necessary for increase in LMA and mesophyll cell surface area (Smes). This allows the leaf to accommodate more chloroplasts, thus increasing A. However, increases in LMA and/or Smes often accompany other changes, such as cell wall thickening, which diminishes gm. Such factors that make the correlations of CC-gm elusive are identified. CONCLUSIONS: For evaluation of contribution of gm to recover CC, leaf life-span is the key factor. The estimation of MC in relation to gm, especially in terms of costs required to regulate aquaporins, could be essential for efficient control of gm over the short-term. Over the long-term, costs are mainly reflected in CC, while benefits also include ultimate fitness attributes in terms of integrated carbon gain over the life of a leaf, plant survival and reproductive output.

    DOI: 10.1093/aob/mcac100

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  7. Root-specific activation of plasma membrane H<SUP>+</SUP>-ATPase 1 enhances plant growth and shoot accumulation of nutrient elements under nutrient-poor conditions in <i>Arabidopsis thaliana</i> Reviewed

    Monden, K; Kamiya, T; Sugiura, D; Suzuki, T; Nakagawa, T; Hachiya, T

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 621   page: 39 - 45   2022.9

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    DOI: 10.1016/j.bbrc.2022.06.097

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  8. Rapid stomatal closure contributes to higher water use efficiency in major C4 compared to C3 Poaceae crops. Reviewed International journal

    Kengo Ozeki, Yoshiyuki Miyazawa, Daisuke Sugiura

    Plant Physiology   Vol. 189 ( 1 ) page: 188 - 203   2022.5

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    Understanding water use characteristics of C3 and C4 crops is important for food security under climate change. Here, we aimed to clarify how stomatal dynamics and water use efficiency (WUE) differ in fluctuating environments in major C3 and C4 crops. Under high and low nitrogen conditions, we evaluated stomatal morphology and kinetics of stomatal conductance (gs) at leaf and whole-plant levels in controlled fluctuating light environments in four C3 and five C4 Poaceae species. We developed a dynamic photosynthesis model, which incorporates C3 and C4 photosynthesis models that consider stomatal dynamics, to evaluate the contribution of rapid stomatal opening and closing to photosynthesis and WUE. C4 crops showed more rapid stomatal opening and closure than C3 crops, which could be explained by smaller stomatal size and higher stomatal density in plants grown at high nitrogen conditions. Our model analysis indicated that accelerating the speed of stomatal closure in C3 crops to the level of C4 crops could enhance WUE up to 16% by reducing unnecessary water loss during low light periods, whereas accelerating stomatal opening only minimally enhanced photosynthesis. The present results suggest that accelerating the speed of stomatal closure in major C3 crops to the level of major C4 crops is a potential breeding target for the realization of water-saving agriculture.

    DOI: 10.1093/plphys/kiac040

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  9. Cross-locational experiments to reveal yield potential and yield-determining factors of the rice cultivar 'Hokuriku 193' and climatic factors to achieve high brown rice yield over 1.2kg m(-2) at Nagano in central inland of Japan Reviewed

    Masaki Okamura, Jun Hosoi, Kenji Nagata, Kentaro Koba, Daisuke Sugiura, Yumiko Arai-Sanoh, Nobuya Kobayashi, Motohiko Kondo

    PLANT PRODUCTION SCIENCE   Vol. 25 ( 2 ) page: 131 - 147   2022.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS LTD  

    Understanding the yield potential and yield-determining factors of recent high-yielding cultivars is essential for further increasing rice yield. In this study, a cross-locational field experiment was conducted across 3 years using 'Hokuriku 193MODIFIER LETTER PRIME (H193), a high-yielding cultivar, at four sites including one in Nagano Prefecture, which is the highest-yielding region in Japan. The highest mean yields of 3 years, 1214 g m(-2) for brown rice grains and 1586 g m(-2) for rough grains, were recorded at the Nagano site. The yields from the 17 environments were strongly correlated with spikelet number per square meter while percentage of filled grain was relatively stable, suggesting that sink capacity is the primary determining factor for grain yield of H193. The climatic factors for high spikelet number at the Nagano site can be explained by the high cumulative radiation before heading associated with longer duration until heading by low night temperature. In addition, a large increase in shoot dry weight during grain filling (Delta W) and high radiation use efficiency (Delta W/rad) at the Nagano site could satisfy large source demand by the large sink size. The high Delta W/rad at the Nagano site associated with low night temperature. This study demonstrated high yield potential of H193 and revealed an environment that achieves extra-high yields in H193, which provided insight to attain further increase in rice yield.[GRAPHICS].

    DOI: 10.1080/1343943X.2021.1981140

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  10. Waterlogged Conditions Influence the Nitrogen, Phosphorus, Potassium, and Sugar Distribution in Sago Palm (Metroxylon sagu Rottb.) at Seedling Stages. Reviewed International journal

    Aidil Azhar, Koki Asano, Daisuke Sugiura, Mana Kano-Nakata, Hiroshi Ehara

    Plants (Basel, Switzerland)   Vol. 11 ( 5 )   2022.3

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    Sago palm (Metroxylon sagu Rottb.) grows in well-drained mineral soil and in peatland with high groundwater levels until complete submersion. However, the published information on nutrient uptake and carbohydrate content in sago palms growing under waterlogging remains unreported. This experiment observed sago palm growth performance under normal soil conditions (non-submerged conditions) as a control plot and extended waterlogged conditions. Several parameters were analyzed: Plant morphological growth traits, nitrogen, phosphorus, potassium, and sugar concentration in the plant organ, including sucrose, glucose, starch, and non-structural carbohydrate. The analysis found that sago palm morphological growth traits were not significantly affected by extended waterlogging. However, waterlogging reduced carbohydrate levels in the upper part of the sago palm, especially the petiole, and increased sugar levels, especially glucose, in roots. Waterlogging also reduced N concentration in roots and leaflets and P in petioles. The K level was independent of waterlogging as the sago palm maintained a sufficient level in all of the plant organs. Long duration waterlogging may reduce the plant's economic value as the starch level in the trunk decreases, although sago palm can grow while waterlogged.

    DOI: 10.3390/plants11050710

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  11. Excessive ammonium assimilation by plastidic glutamine synthetase causes ammonium toxicity in Arabidopsis thaliana. Reviewed International journal

    Takushi Hachiya, Jun Inaba, Mayumi Wakazaki, Mayuko Sato, Kiminori Toyooka, Atsuko Miyagi, Maki Kawai-Yamada, Daisuke Sugiura, Tsuyoshi Nakagawa, Takatoshi Kiba, Alain Gojon, Hitoshi Sakakibara

    Nature communications   Vol. 12 ( 1 ) page: 4944 - 4944   2021.8

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    Plants use nitrate, ammonium, and organic nitrogen in the soil as nitrogen sources. Since the elevated CO2 environment predicted for the near future will reduce nitrate utilization by C3 species, ammonium is attracting great interest. However, abundant ammonium nutrition impairs growth, i.e., ammonium toxicity, the primary cause of which remains to be determined. Here, we show that ammonium assimilation by GLUTAMINE SYNTHETASE 2 (GLN2) localized in the plastid rather than ammonium accumulation is a primary cause for toxicity, which challenges the textbook knowledge. With exposure to toxic levels of ammonium, the shoot GLN2 reaction produced an abundance of protons within cells, thereby elevating shoot acidity and stimulating expression of acidic stress-responsive genes. Application of an alkaline ammonia solution to the ammonium medium efficiently alleviated the ammonium toxicity with a concomitant reduction in shoot acidity. Consequently, we conclude that a primary cause of ammonium toxicity is acidic stress.

    DOI: 10.1038/s41467-021-25238-7

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  12. Genotypic Variation of Endophytic Nitrogen-Fixing Activity and Bacterial Flora in Rice Stem Based on Sugar Content Reviewed

    Takanori Okamoto, Rina Shinjo, Arisa Nishihara, Kazuma Uesaka, Aiko Tanaka, Daisuke Sugiura, Motohiko Kondo

    FRONTIERS IN PLANT SCIENCE   Vol. 12   page: 719259   2021.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FRONTIERS MEDIA SA  

    Enhancement of the nitrogen-fixing ability of endophytic bacteria in rice is expected to result in improved nitrogen use under low-nitrogen conditions. Endophytic nitrogen-fixing bacteria require a large amount of energy to fix atmospheric nitrogen. However, it is unknown which carbon source and bacteria would affect nitrogen-fixing activity in rice. Therefore, this study examined genotypic variations in the nitrogen-fixing ability of rice plant stem as affected by non-structural carbohydrates and endophytic bacterial flora in field-grown rice. In the field experiments, six varieties and 10 genotypes of rice were grown in 2017 and 2018 to compare the acetylene reduction activity (nitrogen-fixing activity) and non-structural carbohydrates (glucose, sucrose, and starch) concentration in their stems at the heading stage. For the bacterial flora analysis, two genes were amplified using a primer set of 16S rRNA and nitrogenase (NifH) gene-specific primers. Next, acetylene reduction activity was correlated with sugar concentration among genotypes in both years, suggesting that the levels of soluble sugars influenced stem nitrogen-fixing activity. Bacterial flora analysis also suggested the presence of common and genotype-specific bacterial flora in both 16S rRNA and nifH genes. Similarly, bacteria classified as rhizobia, such as Bradyrhizobium sp. (Alphaproteobacteria) and Paraburkholderia sp. (Betaproteobacteria), were highly abundant in all rice genotypes, suggesting that these bacteria make major contributions to the nitrogen fixation process in rice stems. Gammaproteobacteria were more abundant in CG14 as well, which showed the highest acetylene reduction activity and sugar concentration among genotypes and is also proposed to contribute to the higher amount of nitrogen-fixing activity.

    DOI: 10.3389/fpls.2021.719259

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  13. International Biological Flora: Nervilia nipponica Reviewed

    Stephan W. Gale, Ayako Maeda, Ayana Miyashita, Daisuke Sugiura, Yuki Ogura-Tsujita, Akihiko Kinoshita, Shohei Fujimori, Michael J. Hutchings, Tomohisa Yukawa

    Journal of Ecology   Vol. 109 ( 7 ) page: 2780 - 2799   2021.7

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    This account presents information on all aspects of the biology of Nervilia nipponica Makino (mukago-saishin) that are relevant to understanding its ecological characteristics and behaviour. The main topics are presented within the standard framework of the International Biological Flora: distribution, habitat, communities, responses to biotic factors, responses to the environment, structure and physiology, phenology, floral and seed characters, herbivores and disease, history, conservation and global heterogeneity.Nervilia nipponica is a small, stoloniferous, seasonally dormant herb that grows in the understorey of evergreen forests in the humid subtropical zone of central and western Japan, with a few outlying populations on Jeju Island in South Korea. Its northern extent is defined by the 0 degrees C winter isotherm, and its occurrence is also limited by site aspect and incline. It is a weak competitor that occupies species-poor microsites in which bare ground and leaf litter predominate. Plant numbers tend to decline as percentage ground cover of surrounding understorey vegetation increases.The inflorescence sprouts from a short-lived, subterranean tuber in late spring and leaf-flush occurs after fruit-set. However, most tubers do not flower in any one annual growth cycle. Long-term monitoring of individually marked plants suggests that tubers are resource-limited and that flowering constrains future genet growth. Nervilia nipponica is exclusively autogamous and has a strong capacity for vegetative propagation. The species is genetically depauperate but exhibits significant differentiation between populations, which comprise clonal clusters in phalanx formation.The level of mycorrhizal infection differs between plant parts and through successive phenological stages. Stable isotope signatures indicate that the species is partially mycoheterotrophic, with fungal partners supporting growth particularly at lower light intensities. Despite this, falling light availability associated with forest succession can lead to population decline.Populations tend to be small and prone to extirpation, but the species is probably under-recorded as a result of its ephemeral emergence above-ground and inconspicuous habit. Management interventions likely to benefit the species at the site level include thinning dense forest canopy and removing encroaching ground cover.

    DOI: 10.1111/1365-2745.13683

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  14. Cell wall thickness and composition are involved in photosynthetic limitation. Reviewed International journal

    Jaume Flexas, María J Clemente-Moreno, Josefina Bota, Tim J Brodribb, Jorge Gago, Yusuke Mizokami, Miquel Nadal, Alicia V Perera-Castro, Margalida Roig-Oliver, Daisuke Sugiura, Dongliang Xiong, Marc Carriquí

    Journal of experimental botany   Vol. 72 ( 11 ) page: 3971 - 3986   2021.5

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    The key role of cell walls in setting mesophyll conductance to CO2 (gm) and, consequently, photosynthesis, is reviewed. First, the theoretical properties of cell walls that can affect gm are presented. Then, we focus on cell wall thickness (Tcw) reviewing empirical evidence showing that Tcw varies strongly among species and phylogenetic groups in a way that correlates with gm and photosynthesis i.e. the thicker the mesophyll cell walls, the lower gm and photosynthesis. Potential interplays of gm, Tcw, dehydration tolerance and hydraulic properties of leaves are also discussed. Dynamic variations of Tcw in response to the environment and their implications in the regulation of photosynthesis are discussed, and recent evidence suggesting an influence of cell wall composition on gm are presented. We then propose a hypothetical mechanism for the influence of cell walls on photosynthesis, combining the effects of thickness and composition, particularly pectins. Finally, we discuss the prospects for using biotechnology for enhancing photosynthesis by means of altering cell wall-related genes.

    DOI: 10.1093/jxb/erab144

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  15. Novel technique for non-destructive LAI estimation by continuous measurement of NIR and PAR in rice canopy Reviewed

    Shota Fukuda, Kentaro Koba, Masaki Okamura, Yuichi Watanabe, Jun Hosoi, Koji Nakagomi, Hideo Maeda, Motohiko Kondo, Daisuke Sugiura

    Field Crops Research   Vol. 263   2021.4

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    Although leaf area index (LAI) is a useful index of crop growth, evaluating LAI by destructive sampling is laborious and time-consuming. In the present study, we developed a novel technique to determine rice LAI non-destructively and accurately throughout growth period in paddy field. Near-infrared radiation (NIR) and photosynthetically active radiation (PAR) were measured by a pair of optical sensors inside and outside rice canopy at 1-min intervals. Simple ratio (SR) and NIR to PAR ratio (N/P) were measured depending on growth stage, and the criteria to extract valid SR and N/P that represent LAI accurately were determined. SR and N/P obtained under cloudy conditions were found to represent LAI with high accuracy (R = 0.91), and seasonal dynamics of canopy growth curves were successfully drawn for two rice cultivars at two experimental fields. Mathematical analysis revealed that not cumulative PAR but cumulative temperature could trigger exponential canopy growth. The present technique can be used as a powerful tool for evaluating, modelling, and phenotyping crop growth characteristics in various rice cultivars as well as other crops. 2

    DOI: 10.1016/j.fcr.2021.108070

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  16. A simple method to observe water distribution in tracheid-bearing wood of subalpine conifer Reviewed

    Haruhiko Taneda, Kenich Yazaki, Tokiyoshi Hiramatsu, Bunnichi Shimizu, Daisuke Sugiura, Yoshiyuki Miyazawa

    TREES-STRUCTURE AND FUNCTION   Vol. 35 ( 2 ) page: 697 - 707   2021.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER HEIDELBERG  

    Key message Distributions of water- and air-filled conduits can be distinguished by observing a xylem cross-section of frozen conifer stem. This method is applicable to field observation in cold winter. Xylem embolism resulting from summer drought and frost drought is one of the critical stresses responsible for the dieback of stems and individuals of subalpine evergreen conifers. The occurrence of xylem embolism in conifers depends not only on the species but also on the plant microhabitats, causing difficulties in understanding possible adaptive strategies against xylem embolism. This study examines a simple method of using a digital camera to photograph the xylem water distribution (CXW method) in cross-sections of frozen stems. Light is transmitted through the water-filled tracheid lumen but reflects and scatters at the surface of embolized tracheids, resulting in contrast in wood color between darker (water-filled tracheids) and lighter (air-filled tracheids) colored regions. The CXW method was effective in detecting water distribution in conifers, although the colored xylem in latewood and reaction wood decreased the color contrast between air- and water-filled regions. By cutting the frozen stem with a cryostat, sequential changes in the water distribution of stem xylem were easily monitored. In the cold winter of the subalpine region, the spatial distribution of embolized conduits can be detected when a branch is collected. If a cryostat is available, this method is applicable to other tracheid-bearing wood collected in any season and does not require additional instruments or time-consuming intensive labor in the field. Information about the hydraulics of conifers growing in extreme environments contributes to the understanding of their adaptive strategy and facilitates accurate prediction of forest dynamics under future climatic conditions.

    DOI: 10.1007/s00468-020-02070-y

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  17. The impact of different soil water levels on nitrogen, phosphorus, and potassium uptake, photosynthetic performance, and sugar distribution of sago palm seedlings Reviewed

    Aidil Azhar, Fitri Audia, Koki Asano, Daigo Makihara, Hitoshi Naito, Daisuke Sugiura, Hiroshi Ehara

    Agrosystems, Geosciences & Environment   Vol. 4 ( 3 )   2021

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    DOI: 10.1002/agg2.20191

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    Other Link: https://onlinelibrary.wiley.com/doi/full-xml/10.1002/agg2.20191

  18. The promoted lateral root 1 (plr1) mutation is involved in reduced basal shoot starch accumulation and increased root sugars for enhanced lateral root growth in rice. Reviewed International journal

    Nonawin Lucob-Agustin, Daisuke Sugiura, Mana Kano-Nakata, Tomomi Hasegawa, Roel R Suralta, Jonathan M Niones, Mayuko Inari-Ikeda, Akira Yamauchi, Yoshiaki Inukai

    Plant science : an international journal of experimental plant biology   Vol. 301   page: 110667 - 110667   2020.12

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    Lateral roots (LRs) are indispensable for plant growth, adaptability and productivity. We previously reported a rice mutant, exhibiting a high density of thick and long LRs (L-type LRs) with long parental roots and herein referred to as promoted lateral root1 (plr1). In this study, we describe that the mutant exhibited decreased basal shoot starch accumulation, suggesting that carbohydrates might regulate the mutant root phenotype. Further analysis revealed that plr1 mutation gene regulated reduced starch accumulation resulting in increased root sugars for the regulation of promoted LR development. This was supported by the exogenous glucose application that promoted L-type LRs. Moreover, nitrogen (N) application was found to reduce basal shoot starch accumulation in both plr1 mutant and wild-type seedlings, which was due to the repressed expression of starch biosynthesis genes. However, unlike the wild-type that responded to N treatment only at seedling stage, the plr1 mutant regulated LR development under low to increasing N levels, both at seedling and higher growth stages. These results suggest that plr1 mutation gene is involved in reduced basal shoot starch accumulation and increased root sugar level for the promotion of L-type LR development, and thus would be very useful in improving rice root architecture.

    DOI: 10.1016/j.plantsci.2020.110667

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  19. A Decrease in Mesophyll Conductance by Cell-Wall Thickening Contributes to Photosynthetic Downregulation(1) Reviewed

    Daisuke Sugiura, Ichiro Terashima, John R. Evans

    PLANT PHYSIOLOGY   Vol. 183 ( 4 ) page: 1600 - 1611   2020.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC PLANT BIOLOGISTS  

    It has been argued that accumulation of nonstructural carbohydrates triggers a decrease in Rubisco content, which downregulates photosynthesis. However, a decrease in the sink-source ratio in several plant species leads to a decrease in photosynthesis and increases in both structural and nonstructural carbohydrate content. Here, we tested whether increases in cell-wall materials, rather than starch content, impact directly on photosynthesis by decreasing mesophyll conductance. We measured various morphological, anatomical, and physiological traits in primary leaves of soybean (Glycine max) and French bean (Phaseolus vulgaris) grown under high- or low-nitrogen conditions. We removed other leaves 2 weeks after sowing to decrease the sink-source ratio and conducted measurements 0, 1, and 2 weeks after defoliation.Cell-wall thickening, rather than starch accumulation, leads to a decrease in mesophyll conductance after a reduction in the sink-source ratio in soybean and French bean.

    DOI: 10.1104/pp.20.00328

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  20. Nitrogen and Water Demands for Maximum Growth of Solanum tuberosum under Doubled CO2: Interaction with Phosphorus Based on the Demands Reviewed

    Yan Yi, Daisuke Sugiura, Katsuya Yano

    ENVIRONMENTAL AND EXPERIMENTAL BOTANY   Vol. 176   2020.8

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    Crop growth promotion utilizing elevated carbon dioxide concentrations (e[CO2]) may be limited by soil nutrient availability. Although numerous studies have suggested the importance of nitrogen (N) for the promotion of growth under e[CO2], N requirement for maximum plant growth is rarely examined. We have found that increase in potato (Solarium tuberosum L.) biomass depends on phosphorus (P) availability under doubled [CO2] conditions. To address whether the N requirement for maximum growth under e[CO2] is dependent on P supply or not in potatoes, we quantified potato growth and water consumption in response to five N supply rates at low P (LP) and high P (HP) conditions. A pot experiment was conducted in controlled-environment chambers with ambient CO2 concentrations (a[CO2]) and an e[CO2] level of double a[CO2]. Foliar critical N concentration per area (critical [N](area)), the minimum N requirement for 90% maximum plant growth, was similar (1.43 g m(-2)) regardless of [CO2] conditions under LP. Under HP, however, the critical [N](area) increased under e[CO2] conditions (1.65 g m(-2)) compared with a[CO2] conditions (1.52 g m(-2)). Water use did not change with e[CO2] under HP conditions, whereas it decreased with e[CO2] under LP conditions despite the increase in biomass owing to higher water-use efficiency (WUE). Although WUE with e[CO2] or HP was independent of N supply, biomass increment with e[CO2] or HP depended on N supply. We concluded that the N and water required by potato plants under e[CO2] would be dependent on P supply. Although under HP, e[CO2] increased N but not water required to obtain maximum growth during the early growth stage, N demand was unchanged and water demand decreased by e[CO2] under LP conditions, probably owing to growth limited by P availability.

    DOI: 10.1016/j.envexpbot.2020.104089

    Web of Science

  21. Comprehensive analysis of the mechanisms underlying enhanced growth and root N acquisition in rice by the endophytic diazotroph, Burkholderia vietnamiensis RS1 Reviewed

    Rina Shinjo, Aiko Tanaka, Daisuke Sugiura, Takamasa Suzuki, Kazuma Uesaka, Yumiko Takebayashi, Mikiko Kojima, Hitoshi Sakakibara, Daigo Takemoto, Motohiko Kondo

    PLANT AND SOIL   Vol. 450 ( 1-2 ) page: 537 - 555   2020.5

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    Aims The aim of this study was to provide a comprehensive understanding of the mechanisms underlying response of rice (Oryza sativa L.) to the endophytic N-2-fixing bacterium Burkholderia vietnamiensis strain RS1 in growth and nitrogen (N) accumulation using transcriptome and hormonome analyses. Methods The effects of B. vietnamiensis RS1 on rice growth and root architecture were examined in pot and field conditions. To identify candidate plant genes that affect the growth of plants inoculated with B. vietnamiensis RS1, transcriptome analysis was conducted. We also examined the hormonal changes by hormonome analysis in relation to the morphological changes in roots. The contribution of N-2 fixation by B. vietnamiensis RS1 was estimated by the acetylene reduction activity (ARA). Results The inoculation of B. vietnamiensis RS1 resulted in increased shoot and root growth of rice plants accompanied by an increased root N absorption rate, especially under high N conditions. The contribution from N-2 fixation was minor because no effect of B. vietnamiensis RS1 on ARA was detected. Transcriptome analysis revealed an increase in the expression of genes related to the transportation and assimilation of N compounds, supporting the enhanced N absorption by roots. The expression of indole-3-acetic acid (IAA) signaling genes and concentrations of IAA increased, which may be related to the increased root development. Conclusions B. vietnamiensis RS1 enhanced rice growth by promoting N accumulation through enhanced root morphological development and N absorption ability; this outcome was probably related to the increased activities of N transportation and assimilation in the presence of a rich N supply.

    DOI: 10.1007/s11104-020-04506-3

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    Scopus

  22. Nitrogen and Water Demands for Maximum Growth of <i>Solanum tuberosum</i> under doubled CO<sub>2</sub>: Interaction with Phosphorus Based on the Demands Reviewed

    Yi Yan, Sugiura Daisuke, Yano Katsuya

    Abstracts of Meeting of the CSSJ   Vol. 249 ( 0 ) page: 131 - 131   2020.3

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:CROP SCIENCE SOCIETY OF JAPAN  

    Crop growth promotion utilizing elevated carbon dioxide concentrations (e[CO2]) may be limited by soil nutrient availability. Although numerous studies have suggested the importance of nitrogen (N) for the promotion of growth under e[CO2], N requirement for maximum plant growth is rarely examined. We have found that increase in potato (Solanum tuberosum L.) biomass depends on phosphorus (P) availability under doubled [CO2] conditions. To address whether the N requirement for maximum growth under e[CO2] is dependent on P supply or not in potatoes, we quantified potato growth and water consumption in response to five N supply rates at low P (LP) and high P (HP) conditions. A pot experiment was conducted in controlled-environment chambers with ambient CO2 concentrations (a[CO2]) and an e[CO2] level of double a[CO2]. Foliar critical N concentration per area (critical [N]area), the minimum N requirement for 90% maximum plant growth, was similar (1.43 g m-2) regardless of [CO2] conditions under LP. Under HP, however, the critical [N]area increased under e[CO2] conditions (1.65 g m-2) compared with a[CO2] conditions (1.52 g m-2). Water use did not change with e[CO2] under HP conditions, whereas it decreased with e[CO2] under LP conditions despite the increase in biomass owing to higher water-use efficiency (WUE). Although WUE with e[CO2] or HP was independent of N supply, biomass increment with e[CO2] or HP depended on N supply. We concluded that the N and water required by potato plants under e[CO2] would be dependent on P supply. Although under HP, e[CO2] increased N but not water required to obtain maximum growth during the early growth stage, N demand was unchanged and water demand decreased by e[CO2] under LP conditions, probably owing to growth limited by P availability.

    DOI: 10.14829/jcsproc.249.0_131

    CiNii Research

  23. Quantifying Phosphorus and Water Demand to Attain Maximum Growth of Solanum tuberosum in a CO2-Enriched Environment Reviewed

    Yan Yi, Daisuke Sugiura, Katsuya Yano

    FRONTIERS IN PLANT SCIENCE   Vol. 10   page: 1417   2019.11

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    Growth promotion by ambient CO2 enrichment may be advantageous for crop growth but this may be influenced by soil nutrient availability. Therefore, we quantified potato (Solanum tuberosum L.) growth responses to phosphorus (P) supply under ambient (a[CO2]) and elevated (doubled) CO2 concentration (e[CO2]). A pot experiment was conducted in controlled-environment chambers with a[CO2] and e[CO2] combined with six P supply rates. We obtained response curves of biomass against P supply rates under a[CO2] and e[CO2] (R-2 = 0.996 and R-2 = 0.992, respectively). A strong interaction between [CO2] and P was found. Overall, e[CO2] enhanced maximum biomass accumulation (1.5-fold) and water-use efficiency (WUE) (1.5-fold), but not total water use. To reach these maxima, minimum P supply rate at both [CO2] conditions was similar. Foliar critical P concentration (i.e., minimum [P] to reach 90% of maximum growth) was also similar at nearly 110 mg P m(-2). Doubling [CO2] did not increase P and water demand of potato plants, thus enabling the promotion of maximum growth without additional P or water supply, but via a significant increase in WUE (9.6 g biomass kg(-1) water transpired), presumably owing to the interaction between CO2 and P.

    DOI: 10.3389/fpls.2019.01417

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    Scopus

    PubMed

  24. Elevated CO2-induced changes in mesophyll conductance and anatomical traits in wild type and carbohydrate-metabolism mutants of Arabidopsis. Reviewed

    Mizokami Y, Sugiura D, Watanabe CKA, Betsuyaku E, Inada N, Terashima I

    Journal of experimental botany   Vol. 70 ( 18 ) page: 4807-4818   2019.9

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    DOI: 10.1093/jxb/erz208

    PubMed

  25. How can we quantitatively study insects whose larvae live beneath the forest floor? A case study at an experimental long-term log-removal site in Japan Reviewed

    Tsunoda Tomonori, Hyodo Fujio, Sugiura Daisuke, Kaneko Nobuhiro, Suzuki Satoshi N.

    ENTOMOLOGICAL SCIENCE   Vol. 22 ( 3 ) page: 275-282   2019.9

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    DOI: 10.1111/ens.12362

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  26. QTL analysis for carbon assimilate translocation-related traits during maturity in rice (<i>Oryza sativa</i> L.). Reviewed

    Phung HD, Sugiura D, Sunohara H, Makihara D, Kondo M, Nishiuchi S, Doi K

    Breeding science   Vol. 69 ( 2 ) page: 289-296   2019.6

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    DOI: 10.1270/jsbbs.18203

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  27. Interspecific differences in how sink-source imbalance causes photosynthetic downregulation among three legume species Reviewed

    Sugiura Daisuke, Betsuyaku Eriko, Terashima Ichiro

    ANNALS OF BOTANY   Vol. 123 ( 4 ) page: 715-726   2019.3

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    DOI: 10.1093/aob/mcy204

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  28. Shoot nitrate underlies a perception of nitrogen satiety to trigger local and systemic signaling cascades in Arabidopsis thaliana Reviewed

    Okamoto Yuki, Suzuki Takamasa, Sugiura Daisuke, Kiba Takatoshi, Sakakibara Hitoshi, Hachiya Takushi

    SOIL SCIENCE AND PLANT NUTRITION   Vol. 65 ( 1 ) page: 56 - 64   2019.1

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    DOI: 10.1080/00380768.2018.1537643

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  29. Effects of elevated atmospheric CO2 on respiratory rates in mature leaves of two rice cultivars grown at a free-air CO2 enrichment site and analyses of the underlying mechanisms.

    Noguchi K, Tsunoda T, Miyagi A, Kawai-Yamada M, Sugiura D, Miyazawa SI, Tokida T, Usui Y, Nakamura H, Sakai H, Hasegawa T

    Plant & cell physiology     2018.2

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    DOI: 10.1093/pcp/pcy017

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  30. Sink-Source Balance and Down-Regulation of Photosynthesis in Raphanus sativus: Effects of Grafting, N and CO2

    Sugiura Daisuke, Watanabe Chihiro K. A., Betsuyaku Eriko, Terashima Ichiro

    PLANT AND CELL PHYSIOLOGY   Vol. 58 ( 12 ) page: 2043-2056   2017.12

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    DOI: 10.1093/pcp/pcx132

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  31. Arabidopsis Phosphatidic Acid Phosphohydrolases Are Essential for Growth under Nitrogen-Depleted Conditions

    Yoshitake Yushi, Sato Ryoichi, Madoka Yuka, Ikeda Keiko, Murakawa Masato, Suruga Ko, Sugiura Daisuke, Noguchi Ko, Ohta Hiroyuki, Shimojima Mie

    FRONTIERS IN PLANT SCIENCE   Vol. 8   2017.10

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    DOI: 10.3389/fpls.2017.01847

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  32. Enhanced leaf photosynthesis as a target to increase grain yield: insights from transgenic rice lines with variable Rieske FeS protein content in the cytochrome b6 /f complex. Reviewed

    Yamori W, Kondo E, Sugiura D, Terashima I, Suzuki Y, Makino A

    Plant, cell & environment   Vol. 39 ( 1 ) page: 80-7   2016.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/pce.12594

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  33. Phytohormonal Regulation of Biomass Allocation and Morphological and Physiological Traits of Leaves in Response to Environmental Changes in Polygonum cuspidatum. Reviewed

    Sugiura D, Kojima M, Sakakibara H

    Frontiers in plant science   Vol. 7   page: 1189   2016

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    DOI: 10.3389/fpls.2016.01189

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  34. Manipulation of the hypocotyl sink activity by reciprocal grafting of two Raphanus sativus varieties: its effects on morphological and physiological traits of source leaves and whole-plant growth Reviewed

    Daisuke Sugiura, Eriko Betsuyaku, Ichiro Terashima

    PLANT CELL AND ENVIRONMENT   Vol. 38 ( 12 ) page: 2629 - 2640   2015.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    To reveal whether hypocotyl sink activities are regulated by the aboveground parts, and whether physiology and morphology of source leaves are affected by the hypocotyl sink activities, we conducted grafting experiments using two Raphanus sativus varieties with different hypocotyl sink activities. Comet (C) and Leafy (L) varieties with high and low hypocotyl sink activities were reciprocally grafted and resultant plants were called by their scion and stock such as CC, LC, CL and LL. Growth, leaf mass per area (LMA), total non-structural carbohydrates (TNCs) and photosynthetic characteristics were compared among them. Comet hypocotyls in CC and LC grew well regardless of the scions, whereas Leafy hypocotyls in CL and LL did not. Relative growth rate was highest in LL and lowest in CC. Photosynthetic capacity was correlated with Rubisco (ribulose 15-bisphosphate carboxylase/oxygenase) content but unaffected by TNC. High C/N ratio and accumulation of TNC led to high LMA and structural LMA. These results showed that the hypocotyl sink activity was autonomously regulated by hypocotyl and that the down-regulation of photosynthesis was not induced by TNC. We conclude that the change in the sink activity alters whole-plant growth through the changes in both biomass allocation and leaf morphological characteristics in R. sativus.

    DOI: 10.1111/pce.12573

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  35. Roles of gibberellins and cytokinins in regulation of morphological and physiological traits in Polygonum cuspidatum responding to light and nitrogen availabilities

    Daisuke Sugiura, Koichiro Sawakami, Mikiko Kojima, Hitoshi Sakakibara, Ichiro Terashima, Masaki Tateno

    FUNCTIONAL PLANT BIOLOGY   Vol. 42 ( 4 ) page: 397 - 409   2015

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    We evaluated the roles of gibberellins (GAs) and cytokinins (CKs) in regulation of morphological traits such as biomass allocation and leaf mass per area (LMA). Seedlings of Polygonum cuspidatum Siebold & Zucc. were grown under various light and N availabilities. We exogenously sprayed solutions of gibberellin (GA(3)), benzyl adenine (BA), uniconazole (an inhibitor of GA biosynthesis) or their mixtures on the aboveground parts, and changes in morphological and physiological traits and relative growth rate (RGR) were analysed. Endogenous levels of GAs and CKs in the control plants were also quantified. The morphological traits were changed markedly by the spraying. Biomass allocation to leaves was increased by GA(3) and BA, whereas it decreased by uniconazole. GA(3) decreased LMA, whereas uniconazole increased it. We found close relationships among morphological and physiological traits such as photosynthetic rate and net assimilation rate, and RGR under all growth conditions. Seedlings with high levels of endogenous GAs or CKs and low levels of endogenous GAs or CKs showed morphologies similar to those sprayed with GA(3) or BA, and those sprayed with uniconazole, respectively. Thus we concluded these phytohormones are involved in the regulation of biomass allocation responding to either light or N availability.

    DOI: 10.1071/FP14212

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    PubMed

  36. Effects of the experimental alteration of fine roots on stomatal conductance and photosynthesis: Case study of devil maple (Acer diabolicum) in a cool temperate region

    Daisuke Sugiura, Masaki Tateno

    ENVIRONMENTAL AND EXPERIMENTAL BOTANY   Vol. 100   page: 105 - 113   2014.4

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    Water is absorbed by fine roots and transported with essential substances to conduct photosynthesis in leaves. To determine how many fine roots are required to absorb adequate water and maximise photosynthesis, the effects of decreasing fine root biomass on stomatal conductance (G(s)) and photosynthetic rate (A) were evaluated using theoretical simulations and laboratory experiments. We used saplings of devil maple (Acer diabolicum), a typical woody species in the cool temperate regions of Japan, grown in high- and low-light environments. A-G(s) relationships and whole-plant hydraulic conductance (K-w) were determined and used for the simulations. Gradual changes in K-w, A, and G(s) were also evaluated with a stepwise decrease in fine roots in the laboratory experiments. The model predicted that K-w, G(s), and A decreased moderately with a decrease in fine roots. For example, A decreased by only 12% when fine roots were decreased by 50%. The model predictions were nearly consistent with the results from laboratory experiments. In conclusion, saplings of devil maple produced fine roots that were more than sufficient in meeting the water demands of photosynthesis. These characteristics may be beneficial in surviving severe drought and in maintaining adequate hydraulic conductance under conditions of moderate water stress. (C) 2014 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.envexpbot.2013.12.020

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  37. High CO2 Triggers Preferential Root Growth of Arabidopsis thaliana Via Two Distinct Systems Under Low pH and Low N Stresses Reviewed

    Takushi Hachiya, Daisuke Sugiura, Mikiko Kojima, Shigeru Sato, Shuichi Yanagisawa, Hitoshi Sakakibara, Ichiro Terashima, Ko Noguchi

    PLANT AND CELL PHYSIOLOGY   Vol. 55 ( 2 ) page: 269 - 280   2014.2

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    Biomass allocation between shoots and roots is an important strategy used by plants to optimize growth in various environments. Root to shoot mass ratios typically increase in response to high CO2, a trend particularly evident under abiotic stress. We investigated this preferential root growth (PRG) in Arabidopsis thaliana plants cultivated under low pH/high CO2 or low nitrogen (N)/high CO2 conditions. Previous studies have suggested that changes in plant hormone, carbon (C) and N status may be related to PRG. We therefore examined the mechanisms underlying PRG by genetically modifying cytokinin (CK) levels, C and N status, and sugar signaling, performing sugar application experiments and determining primary metabolites, plant hormones and expression of related genes. Both low pH/high CO2 and low N/high CO2 stresses induced increases in lateral root (LR) number and led to high C/N ratios; however, under low pH/high CO2 conditions, large quantities of C were accumulated, whereas under low N/high CO2 conditions, N was severely depleted. Analyses of a CK-deficient mutant and a starchless mutant, in conjunction with sugar application experiments, revealed that these stresses induce PRG via different mechanisms. Metabolite and hormone profile analysis indicated that under low pH/high CO2 conditions, excess C accumulation may enhance LR number through the dual actions of increased auxin and decreased CKs.

    DOI: 10.1093/pcp/pcu001

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    PubMed

  38. Concentrative nitrogen allocation to sun-lit branches and the effects on whole-plant growth under heterogeneous light environments Reviewed

    Sugiura D

    Oecologia   Vol. 172 ( 4 ) page: 949-960   2013

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    DOI: 10.1007/s00442-012-2558-7

    Scopus

  39. Long-term, short-interval measurements of the frequency distributions of the photosynthetically active photon flux density and net assimilation rate of leaves in a cool-temperate forest

    Ayana Miyashita, Daisuke Sugiura, Koichiro Sawakami, Ryuji Ichihashi, Tomokazu Tani, Masaki Tateno

    AGRICULTURAL AND FOREST METEOROLOGY   Vol. 152   page: 1 - 10   2012.1

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    Long-term, short-interval measurements of incident photosynthetically active photon flux density (PPFD; mu mol m(-2) s(-1)) on the forest floor are essential for estimating the leaf carbon gain of understory plants. Such PPFD data, however, are scarce. We measured PPFD at 1-min intervals for more than 12 months in cool-temperate forest sites and reported the data as a PPFD frequency distribution. We chose five sites: an open site (OPN), the understory of a deciduous broad-leaved tree stand with no visible gaps (DCD), that of an evergreen conifer stand (EVG), that of a deciduous broad-leaved tree stand with a gap of approximately 80 m(2) (GAPDCD), and that of an evergreen conifer stand with a gap of approximately 100 m(2) (GAPEVG). DCD were divided into three sub sites (DCD1-3) to investigate variation within a small area. GAP-sites were consisted of two sub sites (GAPDCD1-2 and GAPEVG1-2) differing in the distance from the gap center. Using the PPFD data, we estimated the summer seasonal (May-October) net assimilation rate of leaves (NAR(L)) at each site for various photosynthetic capacities (A(max): mu mol m(-2) s(-1)) and other parameters of a light response curve of CO2 assimilation rates. At OPN, the average daily accumulated PPFD (mol m(-2) day(-1)) was highest in May (28.2) and lowest in December (8.2). Even at OPN, the class of instantaneous PPFD that contributed most to NAR(L) was 250-300 mu mol m(-2) s(-1). Such a large contribution of lower PPFD is suggested to be an important feature of a field light-availability. At DCD, the relative PPFD (RPPFD, %) to OPN was 7.2 during canopy closure and 49.4 after leaf shedding (averaged for 3 sites). EVG had the lowest light environment throughout the year. Its average RPPFD was 3%. For GAP sites, summer seasonal RPPFD (%) was 15.6, 18.8, 6.4 and 15.6 for GAPDCD1, GAPDCD2, GAPEVG1 and GAPEVG2, respectively. At OPN, the NAR(L) increased with A(max) (which ranged from 1 to 40), suggesting that plants at OPN do not maximize NAR(L). In contrast, at DCD and EVG, A(max) values were attained that did maximize NAR(L), suggesting that plants at these sites could maximize the NAR(L). A(max)-NAR(L) relationships for GAPDCD and GAPEVG showed similar trend to closed canopy sites, DCD and EVG, while NAR(L) and A(max)* of GAP sites were larger than at these sites. Among DCD1-3, the daily accumulated PPFD (mol m-2 day-1) averaged in summer ranged 1.3-1.8 and the maximum NAR(L) value differed up to 1.5 times. It indicates that A(max) and NAR(L) can be various among plants under a similar canopy conditions. (C) 2011 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.agrformet.2011.08.001

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  40. Optimal Leaf-to-Root Ratio and Leaf Nitrogen Content Determined by Light and Nitrogen Availabilities Reviewed

    Daisuke Sugiura, Masaki Tateno

    PLOS ONE   Vol. 6 ( 7 ) page: e22236   2011.7

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    Plants exhibit higher leaf-to-root ratios (L/R) and lower leaf nitrogen content (N(area)) in low-light than in high-light environments, but an ecological significance of this trait has not been explained from a whole-plant perspective. This study aimed to theoretically and experimentally demonstrate whether these observed L/R and N(area) are explained as optimal biomass allocation that maximize whole-plant relative growth rate (RGR). We developed a model which predicts optimal L/R and N(area) in response to nitrogen and light availability. In the model, net assimilation rate (NAR) was determined by light-photosynthesis curve, light availability measured during experiments, and leaf temperature affecting the photosynthesis and leaf dark respiration rate in high and low-light environments. Two pioneer trees, Morus bombycis and Acer buergerianum, were grown in various light and nitrogen availabilities in an experimental garden and used for parameterizing and testing the model predictions. They were grouped into four treatment groups (relative photosynthetic photon flux density, RPPFD 100% or 10% x nitrogen-rich or nitrogen-poor conditions) and grown in an experimental garden for 60 to 100 days. The model predicted that optimal L/R is higher and N(area) is lower in low-light than high-light environments when compared in the same soil nitrogen availability. Observed L/R and N(area) of the two pioneer trees were close to the predicted optimums. From the model predictions and pot experiments, we conclude that the pioneer trees, M. bombycis and A. buergerianum, regulated L/R and N(area) to maximize RGR in response to nitrogen and light availability.

    DOI: 10.1371/journal.pone.0022236

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    PubMed

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Books 1

  1. 植物と微気象 : 植物生理生態学への定量的なアプローチ

    Jones Hamlyn G., 吉藤 奈津子, 杉浦 大輔, 種子田 春彦, 田中 克典, 鎌倉 真依, 宮澤 真一, 及川 真平, 小杉 緑子, 石田 厚, 長嶋 寿江, 加藤 知道 , 久米 篤 , 大政 謙次 ( Role: Sole author)

    森北出版  2017  ( ISBN:9784627261136

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    Language:Japanese

    CiNii Books

MISC 1

  1. Young Scientist Forum of ACSAC10 ONLINE: Let’s Talk about Young Scientist’s Presents & Futures

    OI Takao, ARATANI Haruka, HONDA Sotaro, IWASA Marina, KAMOSHITA Akihiko, KANO-NAKATA Mana, KATSURA Keisuke, NGUYEN Dinh Thi Ngoc, OJHA Jesish, OKAMOTO Takanori, OUK Rachana, SIWAKOTI Shristi, SUGIURA Daisuke, SULTANZADA Mohammad Marouf, TADA Terufumi, YAMAGUCHI Tomoaki

    Japanese Journal of Crop Science   Vol. 91 ( 3 ) page: 246 - 250   2022.7

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    Language:English   Publishing type:Meeting report   Publisher:CROP SCIENCE SOCIETY OF JAPAN  

    DOI: 10.1626/jcs.91.246

    CiNii Research

Presentations 33

  1. ダイズ葉に蓄積した非構造性炭水化物は気孔開口速度を低下させる"

    小澤佑依, 田中愛子, 鈴木孝征, 杉浦大輔

    日本植物学会第84回大会  2020.9.19 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

  2. 気孔の微気象応答メカニズムから迫るC3・C4作物の水利用戦略

    杉浦大輔、尾関健吾

    日本植物学会第84回大会  2020.9.19 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  3. Elucidation of varietal and regional differences in rice canopy growth to micro climate applying non-destructive LAI measurement method

    Fukuda Shota, Hosoi Jun, Sugiura Daisuke

    Abstracts of Meeting of the CSSJ  2019  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.247.0_213

    CiNii Research

  4. Bacterial flora of nitrogen-fixing endophytes in stem of rice genotypes with different non-structural carbohydrates accumulation

    Okamoto Takanori, Shinjo Rina, Nishihara Arisa, Uesaka Kazuma, Tanaka Aiko, Sugiura Daisuke, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2020  CROP SCIENCE SOCIETY OF JAPAN

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  5. Varietal difference in the response to reductive rhizosphere environment in rice

    Hiramatsu Shuichi, Sugiura Daisuke, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2018  CROP SCIENCE SOCIETY OF JAPAN

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  6. Development of non-destructive LAI measuring technique using NIR/PAR ratio to evaluate rice canopy growth

    Sugiura Daisuke, Fukuda Shota, Hosoi Jun, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2019  CROP SCIENCE SOCIETY OF JAPAN

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    Language:Japanese   Presentation type:Oral presentation (general)  

    DOI: 10.14829/jcsproc.247.0_75

    CiNii Research

  7. Evaluation of water use characteristics in wheat and finger millet under fluctuating light

    Ozeki Kengo, Daisuke Sugiura

    Abstracts of Meeting of the CSSJ  2020.9.3  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.250.0_54

    CiNii Research

  8. Varietal difference in the response to soil redox status in rice

    Hiramatsu Shuichi, Sugiura Daisuke, Nagano Atsushi J., Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2020.3.24  CROP SCIENCE SOCIETY OF JAPAN

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    Language:Japanese   Presentation type:Oral presentation (general)  

    DOI: 10.14829/jcsproc.249.0_95

    CiNii Research

  9. Eco-physiological significance of down- and up-regulation of photosynthesis in soybean and French bean

    Sugiura Daisuke, Kono Masaru, Yamori Wataru, Terashima Ichiro

    Abstracts of Meeting of the CSSJ  2017  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.244.0_56

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  10. Relationship between Endophytic nitrogen fixing ability and Non-structural carbohydrate in rice varieties

    Okamoto Takanori, Shinjo Rina, Tanaka Aiko, Sugiura Daisuke, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2018  CROP SCIENCE SOCIETY OF JAPAN

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  11. イネ<i>plr1</i>変異はデンプン・糖蓄積パターンの変化を通して側根発育を制御する

    Lucob-Agustin Nonawin, 杉浦 大輔, 仲田(狩野) 麻奈, 長谷川 友美, 井成(池田) 真由子, 山内 章, 犬飼 義明

    日本作物学会講演会要旨集  2020  日本作物学会

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  12. Nitrogen and Water Demands for Maximum Growth of <i>Solanum tuberosum</i> under doubled CO<sub>2</sub>: Interaction with Phosphorus Based on the Demands

    Yi Yan, Sugiura Daisuke, Yano Katsuya

    Abstracts of Meeting of the CSSJ  2020.3.24  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.249.0_131

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  13. 4-1-7 Nitrogen and Phosphorus Demands for Maximum Growth of <i>Solanum tuberosum</i> under Doubled CO<sub>2</sub>(4-1 植物の多量栄養素 2020年度岡山大会)

    易 燕, 杉浦 大輔, 矢野 勝也

    日本土壌肥料学会講演要旨集  2020  一般社団法人 日本土壌肥料学会

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  14. Sago Palm for Starch Production and Sustainable Land Development in Peatlands

    Azhar Aidil, Asano Koki, Audia Fitri, Naito Hitoshi, Sugiura Daisuke, Kano-Nakata Mana, Makihara Daigo, Ehara Hiroshi

    Abstracts of Meeting of the CSSJ  2021.3.29  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.251.0_166

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  15. Comparison of methods for calculating mesophyll and chloroplast surface areas facing to intercellular airspaces based on 3D reconstruction models and 2D section images

    Ouk Rachana, Oi Takao, Sugiura Daisuke, Taniguchi Mitsutaka

    Abstracts of Meeting of the CSSJ  2021.3.29  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.251.0_112

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  16. Non-destructive LAI estimation of rice canopy throughout the growing season by continuous measurement of NIR and PAR

    Fukuda Shota, Watanabe Yuichi, Hosoi Jun, Sugiura Daisuke

    Abstracts of Meeting of the CSSJ  2020  CROP SCIENCE SOCIETY OF JAPAN

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  17. Effects of Nitrate on Yield Improvement of High Yielding Indica Rice Cultivars

    Hisamoto Mai, Hotta Yukina, Okamoto Takanori, Shinjo Rina, Sugiura Daisuke, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2022.9.18  CROP SCIENCE SOCIETY OF JAPAN

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    Language:Japanese   Presentation type:Poster presentation  

    DOI: 10.14829/jcsproc.254.0_108

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  18. Microcomputer-controlled water management system for evaluating crop water use characteristics

    Sugiura Daisuke

    Abstracts of Meeting of the CSSJ  2022.3.25  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.253.0_103

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  19. Relationship between salt tolerance and varietal differences in salt stress responses of abscisic acid and hydrogen peroxide accumulation in rice.

    Yamamoto Yumika, Sugiura Daisuke, Yamauchi Akira, Taniguchi Mitsutaka, Mitsuya Shiro

    Abstracts of Meeting of the CSSJ  2022.9.18  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.254.0_15

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  20. 3 連用圃場におけるイネの体内エンドファイト窒素固定能への窒素施肥の影響(中部支部講演会 2021年度支部講演会)

    堀田 幸奈, 岡本 卓哲, 杉浦 大輔, 近藤 始彦

    日本土壌肥料学会講演要旨集  2022.9.5  一般社団法人 日本土壌肥料学会

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    Language:Japanese   Presentation type:Poster presentation  

    DOI: 10.20710/dohikouen.68.0_189_3

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  21. Development of the method for estimating water consumption of C<sub>3</sub>, C<sub>4</sub> cropsusing Micro heat ratio sensor

    Kojima Nagisa, Miyazawa Yoshiyuki, Sugiura Kazuhiko, Sugiura Daisuke

    Abstracts of Meeting of the CSSJ  2023.3.27  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.255.0_118

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  22. 4-1-7 Nitrogen and Phosphorus Demands for Maximum Growth of <i>Solanum tuberosum</i> under Doubled CO<sub>2</sub>(4-1 植物の多量栄養素 2020年度岡山大会)

    易 燕, 杉浦 大輔, 矢野 勝也

    日本土壌肥料学会講演要旨集  2020  一般社団法人 日本土壌肥料学会

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  23. Effects of combined use of nitrate and iron oxide materials on rice yield and soil environment

    Hirokaga Koki, Hisamoto Mai, Kawase Sarasa, Tamura Hinako, Hotta Yukina, Okamoto Takanori, Sugiura Daisuke, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2023.9.12  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.256.0_66

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  24. イネ<i>plr1</i>変異はデンプン・糖蓄積パターンの変化を通して側根発育を制御する

    Lucob-Agustin Nonawin, 杉浦 大輔, 仲田(狩野) 麻奈, 長谷川 友美, 井成(池田, 真由子, 山内 章, 犬飼 義明

    日本作物学会講演会要旨集  2020  日本作物学会

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  25. Accumulation of non-structural carbohydrate and nitrogen-fixing bacterial flora in stem of <i>Oryza glaberrima</i> cv. CG14

    Okamoto Takanori, Hotta Yukina, Shinjo Rina, Nishihara Arisa, Uesaka Kazuma, Tanaka Aiko, Sugiura Daisuke, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2021.3.29  CROP SCIENCE SOCIETY OF JAPAN

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    Language:Japanese   Presentation type:Oral presentation (general)  

    DOI: 10.14829/jcsproc.251.0_82

    CiNii Research

  26. Nitrogen and Water Demands for Maximum Growth of <i>Solanum tuberosum</i> under doubled CO<sub>2</sub>: Interaction with Phosphorus Based on the Demands

    Yi Yan, Sugiura Daisuke, Yano Katsuya

    日本作物学会講演会要旨集  2020  日本作物学会

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    Language:English   Presentation type:Oral presentation (general)  

  27. Relationship between Endophytic nitrogen fixing ability and Non-structural carbohydrate in rice varieties

    Okamoto Takanori, Shinjo Rina, Tanaka Aiko, Sugiura Daisuke, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2018  CROP SCIENCE SOCIETY OF JAPAN

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  28. Analysis of the stomatal opening and closing characteristics and water use in 14 rice varieties under fluctuating light conditions

    Fujino Nagisa, Sugiura Daisuke

    Abstracts of Meeting of the CSSJ  2021.9.8  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.252.0_28

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  29. Analysis of photosynthetic characteristics by hierarchy in <i>sorghum</i> populations

    Ozone Gen, Oi Takao, Kono Yoshiki, Nishiuchi Shunsaku, Sazuka Takashi, Sugiura Daisuke, Taniguchi Mitsutaka

    Abstracts of Meeting of the CSSJ  2023.3.27  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.255.0_115

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  30. Downregulation and delayed induction of photosynthesis by coordinated transcriptomic changes induced by sink-source imbalance

    Sugiura Daisuke, Ozawa Yui, Tanaka Aiko, Suzuki Takamasa

    Abstracts of Meeting of the CSSJ  2023.3.27  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.255.0_112

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  31. Impact of <i>NHX1</i> on water use characteristics of rice under fluctuating light and drought conditions.

    Kataoka Yui, Mitsuya Shiro, Sugiura Daisuke

    Abstracts of Meeting of the CSSJ  2024.3.28  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.257.0_149

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  32. Higher LAI and Lower Night Respiration during Maturity Stage Contribute to Higher yield of High-Yielding Rice Varieties

    Sugiura Daisuke, Okude Satomi, Sato Mio, Okamoto Takanori, Kondo Motohiko, Yumiko Arai-Sanoh, Okamura Masaki

    Abstracts of Meeting of the CSSJ  2024.3.28  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.257.0_19

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  33. The effects of rice straw on nitrogen fixation in paddy fields and rice growth

    Tamura Hinako, Hirokaga Koki, Hisamoto Mai, Kawase Sarasa, Okamoto Takanori, Sugiura Daisuke, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2024.3.28  CROP SCIENCE SOCIETY OF JAPAN

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    DOI: 10.14829/jcsproc.257.0_85

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▼display all

KAKENHI (Grants-in-Aid for Scientific Research) 16

  1. 作物群落レベルのソース・シンク能の新規評価系開発を通じたイネの多収要因解析

    Grant number:23K26887  2024.2 - 2026.3

    科学研究費助成事業  基盤研究(B)

    杉浦 大輔

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    Authorship:Principal investigator 

    Grant amount:\9230000 ( Direct Cost: \7100000 、 Indirect Cost:\2130000 )

  2. Micro controller-based high-throughput measurement system for water use characteristics in major cerial crops.

    Grant number:23K18024  2023.6 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Research (Exploratory)

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    Authorship:Principal investigator 

    Grant amount:\6370000 ( Direct Cost: \4900000 、 Indirect Cost:\1470000 )

  3. Development of a new evaluation system for source and sink capacity in rice canopy

    Grant number:23H02194  2023.4 - 2026.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Principal investigator 

    Grant amount:\18980000 ( Direct Cost: \14600000 、 Indirect Cost:\4380000 )

  4. 過湿ストレス下の炭素動態と養水分吸収能を指標としたダイズ根系形態と機能のモデル化

    Grant number:22KK0084  2022.10 - 2025.3

    日本学術振興会  科学研究費助成事業  国際共同研究加速基金(国際共同研究強化(B))

    高橋 宏和, 野田 祐作, 杉浦 大輔, 野田 祐作, 杉浦 大輔

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    Authorship:Coinvestigator(s) 

    Direct Cost: \1000000 )

    日本においてダイズの耐湿性の向上は,未だ解決されていない長年の課題である.過湿ストレス下において植物の根は真っ先にその影響を受ける器官であることから,耐湿性向上には過湿ストレスに適応できる根系を育種する必要がある.そこで,植物の根が過湿ストレス後の根系形成過程を、これまでのような形態的な特徴だけではなく,①植物体内の生理応答、②根系の再構築,③根系機能の回復の3段階に分けて解析を行い,ダイズの過湿ストレスに対する根系形成を理解する.さらにこれらの情報を利用して,CIRADにおける海外共同研究者と連携して過湿ストレスに対するダイズの根系形成モデルを構築することで,根型育種における問題解決に挑む.
    令和2年度の日本におけるダイズは飼料用も含めるとわずか6%程度となっている.ダイズの生産性が向上しない要因の一つに過湿ストレスが挙げられる.日本においてダイズの耐湿性の向上は,未だ解決されていない長年の課題である.植物の根は,養水分の吸収を担う重要な器官であるとともに,湿害,旱害,塩害,貧栄養などの環境ストレスに真っ先に晒される器官であることから,作物はこのようなストレスに適応するために根系の再構築を行う.そのため,植物が環境ストレス耐性を獲得するためには,根系の改良が重要であり,第二の緑の革命は根型育種において起こると期待されている.そのため,耐湿性向上においても過湿ストレスに適応できる根系を育種する必要がある.そのためには,過湿ストレスに対する根系形成を理解し,根系形成モデル構築し,根型育種のための明確なストラテジーの確立が必要不可欠である.申請者らは,これまでに過湿ストレス下において,根系形態の変化よりもはるかに早く炭素動態を変化させていることを明らかにした.そこで本申請課題では,植物の根が過湿ストレス後の根系形成過程を、これまでのような形態的な特徴だけではなく,①植物体内の生理応答、②根系の再構築,③根系機能の回復の3段階に分けて解析を行い,ダイズの過湿ストレスに対する根系形成を理解する.さらにこれらの情報を利用して,CIRADにおける海外共同研究者と連携して過湿ストレスに対するダイズの根系形成モデルの構築することで,根型育種における問題解決に挑む.
    過湿ストレス,根系形態,炭素動態,水利用効率についてその関連性が明確になりつつあるが,昨年度の電気代高騰により,高崎量子応用研究所におけるポジトロンイメージングが実施できていない.しかし,2023年度においては,解析を行う目処が立っている.また,新型コロナウイルスの影響でCIRADの設備が十分に稼働しておらず,2022年度は栽培実験が行えなかった.しかしこちらも,先方との調整により2023年度は栽培実験が行える時期を確保した。
    2022年度は新型コロナウイルスの影響でCIRADの設備が十分に稼働しておらず,栽培試験を行うことができなかった.しかし,CIRADの共同研究者との調整により2023年度に使用するダイズ系統数を増やし解析を行うこととなった.これにり,9月から1ヶ月間複数のダイズ品種における過湿ストレス下の炭素動態や水利用効率,根系形態を評価する.また,日本における解析においても,引き続き,ダイズにおいて,過湿ストレス,根系形態,炭素動態,水利用効率についてその関連性についての解析を行う.特に,炭素動態は過湿ストレス後30分以内に変化し,過湿ストレス下では炭素輸送が阻害されることが明らかとなり,さらに,ストレス後18時間ごろにこの阻害が一部解除されることが明らかとなった.このことは,本研究課題における過湿ストレス後の根系形成過程には①植物体内の生理応答、②根系の再構築,③根系機能の回復の3段階があるという仮説を支持するものであった.そこで,2023年度は,リアルタイムイメージングを用いてさらに炭素動態を詳細に調査することで,この仮説に関する知見を確かなものにしていく.また,得られた根系形態,炭素動態の情報を用いて根系形成モデルの構築を試験する.

  5. Strengthening rice ripening ability through a multifaceted approach

    Grant number:22H02310  2022.4 - 2026.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s)  Grant type:Competitive

  6. Physiological and microbial analysis on endophytic N fixation in rice stem and its improvement

    Grant number:22H02324  2022.4 - 2026.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s) 

    Direct Cost: \100000 )

  7. Study on the molecular mechanism of salt removal ability in rice leaf sheath and its physiological role under salinity

    Grant number:22H02325  2022.4 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s)  Grant type:Competitive

  8. Ecophysiological study on tree mortality of conifer trees in a fir wave

    Grant number:21H02228  2021.4 - 2025.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s) 

  9. Non-destructive and continuous measurement of rice growth for elucidate yield-limiting factors of high-yielding rice cultivars

    Grant number:20H02965  2020.4 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Sugiura Daisuke

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    Authorship:Principal investigator 

    Grant amount:\17940000 ( Direct Cost: \13800000 、 Indirect Cost:\4140000 )

    We aimed to elucidate the yield determining factors of the high-yielding rice cultivars, Hokuriku 193 and Oonari, through cross-locational experiments in Japan (Nagoya, Tsukuba, Joetsu, Nagano). We developed a novel technique for non-destructive leaf area index (LAI) estimation of rice from transplanting to harvest using near-infrared light (NIR) and photosynthetically active radiation (PAR). Additionally, we developed a system to measure the nighttime respiration rate of rice plants and quantified the temperature dependence of respiration. Based on these results, it is suggested that the highest yield in Nagano was due to the maintenance of LAI until the late growth stage and the low nighttime temperatures that suppress respiration rate.

  10. Physiological mechanisms of carbohydrate utilization by N fixing endophyte for improving N fixation capacity in rice

    Grant number:19H02941  2019.4 - 2023.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Kondo Motohiko

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    Authorship:Coinvestigator(s) 

    We analyzed the physiological and genetic factors related to the variation and improvement of nitrogen-fixing activity by nitrogen-fixing endophytes in rice plants. We found that stem nitrogen fixation activity is affected by the accumulation of NSCs, especially free sugars, and that activity is high in African rice CG14 and other rice cultivars. The involvement of sugars was also verified by analyzing starch synthesis mutant lines and by using shading. Furthermore, we analyzed the flora in the stem of rice cultivars with different nitrogen-fixing ability, and found that α- and β-proteobacteria were the main nitrogen-fixing bacteria. The results of the evaluation of the effect of nitrogen fertilization on the nitrogen fixation capacity in the field showed that the contribution to the total nitrogen absorption was relatively small, but nitrogen fertilization did not have a clear effect on the cumulative nitrogen fixation capacity.

  11. Screening mutant lines showing enhanced water use efficiencies with a newly developed system for its visualization

    Grant number:18KK0170  2018.10 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

    Yamori Wataru

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    Authorship:Coinvestigator(s) 

    Research on improving the water use efficiency of plants is essential. In this research project, basic research was conducted to create plants that show high productivity under water-saving conditions. By combining chlorophyll fluorescence and thermography, we succeeded to construct a prototype of a visualization device for plant water use efficiency, which can simultaneously evaluate photosynthetic rate and transpiration rate. In addition, basic research for genetic improvement of water use efficiency in plants led to the discovery of several factors effective in enhancing plant productivity under water-saving conditions.

  12. Responses of gene expressions in rice plants to micro-meteorological conditions; their physiological role in absorption of water and nutrients from roots

    Grant number:17H03896  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    KUWAGATA Tsuneo

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    Authorship:Coinvestigator(s) 

    We experimentally investigated how micro-meteorological conditions influence expression levels of the functional genes which contribute to absorption of water and nutrients by the rice crops, and examined the physiological roles of the environmental responses of these genes to the growth of rice. The experimental results indicated that the expression levels of many kinds of aquaporin genes both in roots and leaves increased with the transpirational demand during the all growth stages, and that expression level of about half of all genes in the rice leaves had a positive (or negative) correlation with transpiration in the early ripening period. Finally, we demonstrated that the environmental response of functional genes to day-to-day variation in micro-meteorological conditions could play important roles in several eco-physiological processes, such as physiological response of roots to decrease in soil water content and change in water use efficiency with atmospheric CO2 concentration.

  13. Investigation of interspecific differences in regulatory mechanism of photosynthesis by spectroscopic imaging and microclimate analysis

    Grant number:17K15192  2017.4 - 2020.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Young Scientists (B)

    Sugiura Daisuke

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    Authorship:Principal investigator 

    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

    This study aimed at elucidating responses of photosynthetic characteristics and canopy growth to micro-climate and their interspecific or intercultivar differences in crops. Physiological mechanisms of down-regulation of photosynthesis responding to non-structural carbohydrates in soybean and French bean were closely examined. We also developed a technique to estimate rice canopy growth non-destructively by measuring near infra-red radiation (NIR) and photosynthetically active radiation (PAR) inside and outside rice canopy. This technique allowed us to trace seasonal dynamics of canopy growth from transplanting to maturity stage and to evaluate varietal and regional differences in the canopy growth characteristics.

  14. 植物の個体レベルのC・N分配調節メカニズムに関する生理生態学的研究

    Grant number:14J07443  2014.4 - 2017.3

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

    杉浦 大輔

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    植物体の光合成産物のシンク活性に対して、光合成器官である葉のソース活性が過剰なとき、葉に非構造性炭水化物 (TNC、デンプンや可溶性糖) が蓄積すると、光合成速度が低下する現象は、光合成ダウンレギュレーションとして知られている。しかし、その詳細なメカニズムや種間差、生態学的意義については不明な点が多かった。本研究ではこれらの問題を解決するために、以下の実験を行った。
    アブラナ科植物5種、マメ科植物3種を用いた環境操作実験を行い、TNCが光合成速度に与える影響を評価した。その結果、TNCの蓄積による光合成速度の低下は、マメ科植物のインゲンのみにおいてしか見られなかった。
    ダイズとインゲンを用いた詳細な比較実験から、シンク・ソース比の減少に応じて、葉にTNCだけでなく、細胞壁などの構造性炭水化物も蓄積することが分かった。そこで、細胞壁量の増加が細胞壁厚さによるものであれば、葉内から葉肉細胞内へのCO2透過性(葉肉コンダクタンス)も減少していると仮説を立て、炭素安定同位体方による葉肉コンダクタンスの測定と、透過型電子顕微鏡による細胞壁厚さの解析を行った。その結果、両種において、シンク・ソース比の減少に応じて細胞壁厚さが3倍近く増加していた。さらに、葉肉コンダクタンスと細胞壁量・厚さの間には明確な負の相関がみられた。
    これらの結果から、シンク・ソース比の減少に応じた光合成ダウンレギュレーションにおいては、ルビスコなどの光合成タンパク質の減少による生理学的な調節だけでなく、細胞壁厚さの増加による形態的な調節も行われていることが示された。

  15. 植物の個体レベルのC・N分配調節メカニズムに関する生理生態学的研究

    Grant number:25891011  2013.8 - 2015.3

    日本学術振興会  科学研究費助成事業  研究活動スタート支援

    杉浦 大輔

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    Authorship:Principal investigator 

    Grant amount:\2730000 ( Direct Cost: \2100000 、 Indirect Cost:\630000 )

    植物は個体レベルの葉、茎、根への炭水化物 (C) や窒素 (N) の分配や、葉の面積あたりの重さ (LMA) を、環境条件に応じて変化させる。本研究では、このようなC・N分配パターンや、LMAを調節するメカニズムについて、植物ホルモンであるジベレリン (GA) やサイトカイニン (CK) の役割や、シンクソースバランスに応じて葉に蓄積する糖が果たす役割を解明することを目的として行った。
    (1) 草本植物イタドリ (Polygonum cuspidatum) を用いて、光やN条件の変化に応じた個体レベルのC分配の変化と、内生GAやCKの変化の関係を解析した。強光・弱光の各条件下では、高N条件ほど、地上部全体のCK濃度が高く、同時に個体レベルの葉へのC分配比も高かった。活性型GA濃度も同様に高N条件ほど高く、強光・低N条件ではほとんど検出されなかった。外生GA、CK添加時に見られる葉へのC分配比と、内生GA、CK濃度が高いときに見られる葉へのC分配比は同様の傾向を示すため、これらの植物ホルモンが個体レベルのC分配の変化を調節するメカニズムに深く関わっていることが示唆された。
    (2) 草本植物ダイコン (Raphanus sativus) の、胚軸が肥大する品種 (コメット:貯蔵シンク活性が高い) と肥大しない品種 (葉大根:活性が低い) を接ぎ木することでシンクソースバランスを変化させ、葉へ蓄積する糖がLMAや葉の構造に与える影響を評価した。どちらの品種でも、貯蔵シンク活性が低い葉大根の胚軸を台にすると、シンク活性が高いコメットを台にした時と比べ、LMAが大幅に増加した。増加分はデンプンだけでは説明できず、葉の厚さはほとんど変化していなかったため、デンプンが蓄積するほど細胞壁成分が増加している可能性が示された。葉の横断切片の構造解析を行ったところ、細胞数や細胞密度がデンプン量に応じて変化していることが分かった。
    翌年度、交付申請を辞退するため、記入しない。
    翌年度、交付申請を辞退するため、記入しない。

  16. 複合的環境要因に応じた植物個体レベルの物質分配調節メカニズムの探索

    Grant number:11J09850  2011 - 2012

    日本学術振興会  科学研究費助成事業  特別研究員奨励費

    杉浦 大輔

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    植物個体レベルの葉/根比(L/R)、葉の厚さ(LMA)や葉面積あたりのN濃度(Narea)などの炭素(C)・窒素(N)分配パターンは、光環境に応じた葉のN需要および土壌からのN供給に応じて、相対成長速度(RGR)を最大化させるように調節されていることが示唆されている(Sugiura & Tateno 2011)。本研究では、このような環境変化に応じたCN分配パターンを制御するメカニズムについて、植物ホルモンの役割に焦点を当て、(実験1)外生植物ホルモンの添加実験、(実験2)環境変化に応じた内生植物ホルモンの定量的解析、を行なった。
    (実験1)において、外生植物ホルモンとして、ジベレリン(G,GA3)、サイトカイニン(B,ベンジルアデニン)、ジベレリン合成阻害剤(U,ウニコナゾール)を用い、これらを単体、または組み合わせて植物体全体に添加する実験を行った。これらの植物ホルモン処理によって、個体レベルでの葉面積あたりの葉の重さ(LMA,gm-2)やL/Rが大きく変化した。また、このような形態的特性の変化と連動して葉のNareaが変化し、光合成速度や純生産速度といった生理的特性が変化した。個体全体のRGRは主に形態的特性の変化で説明されたことから、環境変化に応じたこれらの植物ホルモンによる形態的特性の制御によって、RGRが決定されていることが示唆された。
    (実験2)では、イタドリを、コントロール群は3段階のN条件(HN、MN、LN)で生育させたのち、HNはさらに摘葉(Def)、低N(LowN)、弱光(LowL)処理をし、1週間生育させた。これらのグループごとに、葉位ごとの、生理的形質、CN含量、バイオマス分配、内生の植物ホルモン量(GAs:ジベレリン、CKs=サイトカイニン、IAA:オーキシン)の測定を行った。コントロール群では、N条件が高いほど葉/根比が大きく、GAs量も高かった。処理群では、Def、LowL、LowNの順に、葉へのバイオマス分配比が大きいほど、GAs重も高かった。これらの結果からも、植物体はN条件や光条件の変化に対して、内生植物ホルモンの量を変化させることで葉根比を調節している可能性が示唆された.

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Teaching Experience (On-campus) 8

  1. 生命農学演習4

    2022

  2. 生命農学演習3

    2022

  3. 生命農学演習2

    2022

  4. 生命農学演習1

    2022

  5. 修士論文研究4

    2022

  6. 修士論文研究3

    2022

  7. 修士論文研究2

    2022

  8. 修士論文研究1

    2022

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