Updated on 2022/08/16

写真a

 
SUGIURA Daisuke
 
Organization
Graduate School of Bioagricultural Sciences Department of Plant Production Sciences Lecturer
Graduate School
Graduate School of Bioagricultural Sciences
Undergraduate School
School of Agricultural Sciences Department of Bioresource Sciences
Title
Lecturer
External link

Degree 1

  1. 博士(理学) ( 2012.3   東京大学 ) 

Research Areas 4

  1. Environmental Science/Agriculture Science / Crop production science

  2. Life Science / Ecology and environment

  3. Life Science / Ecology and environment

  4. Environmental Science/Agriculture Science / Crop production science

Professional Memberships 3

  1. Crop Science Society of Japan

  2. The Botanical Society of Japan

  3. The Ecological Society of Japan

 

Papers 34

  1. Cost-benefit analysis of mesophyll conductance - Diversities of anatomical, biochemical and environmental determinants. Reviewed

    Mizokami Y, Oguchi R, Sugiura D, Yamori W, Noguchi K, Terashima I

    Annals of botany     2022.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/aob/mcac100

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  2. Root-specific activation of plasma membrane H<sup>+</sup>-ATPase 1 enhances plant growth and shoot accumulation of nutrient elements under nutrient-poor conditions in Arabidopsis thaliana. Reviewed

    Monden K, Kamiya T, Sugiura D, Suzuki T, Nakagawa T, Hachiya T

    Biochemical and biophysical research communications   Vol. 621   page: 39 - 45   2022.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2022.06.097

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  3. Waterlogged Conditions Influence the Nitrogen, Phosphorus, Potassium, and Sugar Distribution in Sago Palm (Metroxylon sagu Rottb.) at Seedling Stages Reviewed

    Azhar Aidil, Asano Koki, Sugiura Daisuke, Kano-Nakata Mana, Ehara Hiroshi

    PLANTS-BASEL   Vol. 11 ( 5 )   2022.3

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/plants11050710

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  4. Rapid stomatal closure contributes to higher water use efficiency in major C4 compared to C3 Poaceae crops. Reviewed

    Ozeki K, Miyazawa Y, Sugiura D

    Plant physiology     2022.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/plphys/kiac040

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  5. Cross-locational experiments to reveal yield potential and yield-determining factors of the rice cultivar 'Hokuriku 193' and climatic factors to achieve high brown rice yield over 1.2kg m(-2) at Nagano in central inland of Japan Reviewed

    Masaki Okamura, Jun Hosoi, Kenji Nagata, Kentaro Koba, Daisuke Sugiura, Yumiko Arai-Sanoh, Nobuya Kobayashi, Motohiko Kondo

    PLANT PRODUCTION SCIENCE     2021.10

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:TAYLOR & FRANCIS LTD  

    Understanding the yield potential and yield-determining factors of recent high-yielding cultivars is essential for further increasing rice yield. In this study, a cross-locational field experiment was conducted across 3 years using 'Hokuriku 193MODIFIER LETTER PRIME (H193), a high-yielding cultivar, at four sites including one in Nagano Prefecture, which is the highest-yielding region in Japan. The highest mean yields of 3 years, 1214 g m(-2) for brown rice grains and 1586 g m(-2) for rough grains, were recorded at the Nagano site. The yields from the 17 environments were strongly correlated with spikelet number per square meter while percentage of filled grain was relatively stable, suggesting that sink capacity is the primary determining factor for grain yield of H193. The climatic factors for high spikelet number at the Nagano site can be explained by the high cumulative radiation before heading associated with longer duration until heading by low night temperature. In addition, a large increase in shoot dry weight during grain filling (Delta W) and high radiation use efficiency (Delta W/rad) at the Nagano site could satisfy large source demand by the large sink size. The high Delta W/rad at the Nagano site associated with low night temperature. This study demonstrated high yield potential of H193 and revealed an environment that achieves extra-high yields in H193, which provided insight to attain further increase in rice yield.[GRAPHICS].

    DOI: 10.1080/1343943X.2021.1981140

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  6. Excessive ammonium assimilation by plastidic glutamine synthetase causes ammonium toxicity in Arabidopsis thaliana. Reviewed International journal

    Takushi Hachiya, Jun Inaba, Mayumi Wakazaki, Mayuko Sato, Kiminori Toyooka, Atsuko Miyagi, Maki Kawai-Yamada, Daisuke Sugiura, Tsuyoshi Nakagawa, Takatoshi Kiba, Alain Gojon, Hitoshi Sakakibara

    Nature communications   Vol. 12 ( 1 ) page: 4944 - 4944   2021.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Plants use nitrate, ammonium, and organic nitrogen in the soil as nitrogen sources. Since the elevated CO2 environment predicted for the near future will reduce nitrate utilization by C3 species, ammonium is attracting great interest. However, abundant ammonium nutrition impairs growth, i.e., ammonium toxicity, the primary cause of which remains to be determined. Here, we show that ammonium assimilation by GLUTAMINE SYNTHETASE 2 (GLN2) localized in the plastid rather than ammonium accumulation is a primary cause for toxicity, which challenges the textbook knowledge. With exposure to toxic levels of ammonium, the shoot GLN2 reaction produced an abundance of protons within cells, thereby elevating shoot acidity and stimulating expression of acidic stress-responsive genes. Application of an alkaline ammonia solution to the ammonium medium efficiently alleviated the ammonium toxicity with a concomitant reduction in shoot acidity. Consequently, we conclude that a primary cause of ammonium toxicity is acidic stress.

    DOI: 10.1038/s41467-021-25238-7

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  7. International Biological Flora: Nervilia nipponica Reviewed

    Stephan W. Gale, Ayako Maeda, Ayana Miyashita, Daisuke Sugiura, Yuki Ogura-Tsujita, Akihiko Kinoshita, Shohei Fujimori, Michael J. Hutchings, Tomohisa Yukawa

    Journal of Ecology   Vol. 109 ( 7 ) page: 2780 - 2799   2021.7

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1111/1365-2745.13683

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  8. Cell wall thickness and composition are involved in photosynthetic limitation. Reviewed International journal

    Jaume Flexas, María J Clemente-Moreno, Josefina Bota, Tim J Brodribb, Jorge Gago, Yusuke Mizokami, Miquel Nadal, Alicia V Perera-Castro, Margalida Roig-Oliver, Daisuke Sugiura, Dongliang Xiong, Marc Carriquí

    Journal of experimental botany   Vol. 72 ( 11 ) page: 3971 - 3986   2021.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    The key role of cell walls in setting mesophyll conductance to CO2 (gm) and, consequently, photosynthesis, is reviewed. First, the theoretical properties of cell walls that can affect gm are presented. Then, we focus on cell wall thickness (Tcw) reviewing empirical evidence showing that Tcw varies strongly among species and phylogenetic groups in a way that correlates with gm and photosynthesis i.e. the thicker the mesophyll cell walls, the lower gm and photosynthesis. Potential interplays of gm, Tcw, dehydration tolerance and hydraulic properties of leaves are also discussed. Dynamic variations of Tcw in response to the environment and their implications in the regulation of photosynthesis are discussed, and recent evidence suggesting an influence of cell wall composition on gm are presented. We then propose a hypothetical mechanism for the influence of cell walls on photosynthesis, combining the effects of thickness and composition, particularly pectins. Finally, we discuss the prospects for using biotechnology for enhancing photosynthesis by means of altering cell wall-related genes.

    DOI: 10.1093/jxb/erab144

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  9. Novel technique for non-destructive LAI estimation by continuous measurement of NIR and PAR in rice canopy Reviewed

    Shota Fukuda, Kentaro Koba, Masaki Okamura, Yuichi Watanabe, Jun Hosoi, Koji Nakagomi, Hideo Maeda, Motohiko Kondo, Daisuke Sugiura

    Field Crops Research   Vol. 263   2021.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Although leaf area index (LAI) is a useful index of crop growth, evaluating LAI by destructive sampling is laborious and time-consuming. In the present study, we developed a novel technique to determine rice LAI non-destructively and accurately throughout growth period in paddy field. Near-infrared radiation (NIR) and photosynthetically active radiation (PAR) were measured by a pair of optical sensors inside and outside rice canopy at 1-min intervals. Simple ratio (SR) and NIR to PAR ratio (N/P) were measured depending on growth stage, and the criteria to extract valid SR and N/P that represent LAI accurately were determined. SR and N/P obtained under cloudy conditions were found to represent LAI with high accuracy (R = 0.91), and seasonal dynamics of canopy growth curves were successfully drawn for two rice cultivars at two experimental fields. Mathematical analysis revealed that not cumulative PAR but cumulative temperature could trigger exponential canopy growth. The present technique can be used as a powerful tool for evaluating, modelling, and phenotyping crop growth characteristics in various rice cultivars as well as other crops. 2

    DOI: 10.1016/j.fcr.2021.108070

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  10. A simple method to observe water distribution in tracheid-bearing wood of subalpine conifer Reviewed

    Haruhiko Taneda, Kenich Yazaki, Tokiyoshi Hiramatsu, Bunnichi Shimizu, Daisuke Sugiura, Yoshiyuki Miyazawa

    TREES-STRUCTURE AND FUNCTION   Vol. 35 ( 2 ) page: 697 - 707   2021.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER HEIDELBERG  

    Key message Distributions of water- and air-filled conduits can be distinguished by observing a xylem cross-section of frozen conifer stem. This method is applicable to field observation in cold winter. Xylem embolism resulting from summer drought and frost drought is one of the critical stresses responsible for the dieback of stems and individuals of subalpine evergreen conifers. The occurrence of xylem embolism in conifers depends not only on the species but also on the plant microhabitats, causing difficulties in understanding possible adaptive strategies against xylem embolism. This study examines a simple method of using a digital camera to photograph the xylem water distribution (CXW method) in cross-sections of frozen stems. Light is transmitted through the water-filled tracheid lumen but reflects and scatters at the surface of embolized tracheids, resulting in contrast in wood color between darker (water-filled tracheids) and lighter (air-filled tracheids) colored regions. The CXW method was effective in detecting water distribution in conifers, although the colored xylem in latewood and reaction wood decreased the color contrast between air- and water-filled regions. By cutting the frozen stem with a cryostat, sequential changes in the water distribution of stem xylem were easily monitored. In the cold winter of the subalpine region, the spatial distribution of embolized conduits can be detected when a branch is collected. If a cryostat is available, this method is applicable to other tracheid-bearing wood collected in any season and does not require additional instruments or time-consuming intensive labor in the field. Information about the hydraulics of conifers growing in extreme environments contributes to the understanding of their adaptive strategy and facilitates accurate prediction of forest dynamics under future climatic conditions.

    DOI: 10.1007/s00468-020-02070-y

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  11. The impact of different soil water levels on nitrogen, phosphorus, and potassium uptake, photosynthetic performance, and sugar distribution of sago palm seedlings Reviewed

    Aidil Azhar, Fitri Audia, Koki Asano, Daigo Makihara, Hitoshi Naito, Daisuke Sugiura, Hiroshi Ehara

    Agrosystems, Geosciences & Environment   Vol. 4 ( 3 )   2021

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:Wiley  

    DOI: 10.1002/agg2.20191

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  12. Genotypic Variation of Endophytic Nitrogen-Fixing Activity and Bacterial Flora in Rice Stem Based on Sugar Content Reviewed

    Takanori Okamoto, Rina Shinjo, Arisa Nishihara, Kazuma Uesaka, Aiko Tanaka, Daisuke Sugiura, Motohiko Kondo

    FRONTIERS IN PLANT SCIENCE   Vol. 12   page: 719259   2021

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:FRONTIERS MEDIA SA  

    Enhancement of the nitrogen-fixing ability of endophytic bacteria in rice is expected to result in improved nitrogen use under low-nitrogen conditions. Endophytic nitrogen-fixing bacteria require a large amount of energy to fix atmospheric nitrogen. However, it is unknown which carbon source and bacteria would affect nitrogen-fixing activity in rice. Therefore, this study examined genotypic variations in the nitrogen-fixing ability of rice plant stem as affected by non-structural carbohydrates and endophytic bacterial flora in field-grown rice. In the field experiments, six varieties and 10 genotypes of rice were grown in 2017 and 2018 to compare the acetylene reduction activity (nitrogen-fixing activity) and non-structural carbohydrates (glucose, sucrose, and starch) concentration in their stems at the heading stage. For the bacterial flora analysis, two genes were amplified using a primer set of 16S rRNA and nitrogenase (NifH) gene-specific primers. Next, acetylene reduction activity was correlated with sugar concentration among genotypes in both years, suggesting that the levels of soluble sugars influenced stem nitrogen-fixing activity. Bacterial flora analysis also suggested the presence of common and genotype-specific bacterial flora in both 16S rRNA and nifH genes. Similarly, bacteria classified as rhizobia, such as Bradyrhizobium sp. (Alphaproteobacteria) and Paraburkholderia sp. (Betaproteobacteria), were highly abundant in all rice genotypes, suggesting that these bacteria make major contributions to the nitrogen fixation process in rice stems. Gammaproteobacteria were more abundant in CG14 as well, which showed the highest acetylene reduction activity and sugar concentration among genotypes and is also proposed to contribute to the higher amount of nitrogen-fixing activity.

    DOI: 10.3389/fpls.2021.719259

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  13. The promoted lateral root 1 (plr1) mutation is involved in reduced basal shoot starch accumulation and increased root sugars for enhanced lateral root growth in rice. Reviewed International journal

    Nonawin Lucob-Agustin, Daisuke Sugiura, Mana Kano-Nakata, Tomomi Hasegawa, Roel R Suralta, Jonathan M Niones, Mayuko Inari-Ikeda, Akira Yamauchi, Yoshiaki Inukai

    Plant science : an international journal of experimental plant biology   Vol. 301   page: 110667 - 110667   2020.12

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    Lateral roots (LRs) are indispensable for plant growth, adaptability and productivity. We previously reported a rice mutant, exhibiting a high density of thick and long LRs (L-type LRs) with long parental roots and herein referred to as promoted lateral root1 (plr1). In this study, we describe that the mutant exhibited decreased basal shoot starch accumulation, suggesting that carbohydrates might regulate the mutant root phenotype. Further analysis revealed that plr1 mutation gene regulated reduced starch accumulation resulting in increased root sugars for the regulation of promoted LR development. This was supported by the exogenous glucose application that promoted L-type LRs. Moreover, nitrogen (N) application was found to reduce basal shoot starch accumulation in both plr1 mutant and wild-type seedlings, which was due to the repressed expression of starch biosynthesis genes. However, unlike the wild-type that responded to N treatment only at seedling stage, the plr1 mutant regulated LR development under low to increasing N levels, both at seedling and higher growth stages. These results suggest that plr1 mutation gene is involved in reduced basal shoot starch accumulation and increased root sugar level for the promotion of L-type LR development, and thus would be very useful in improving rice root architecture.

    DOI: 10.1016/j.plantsci.2020.110667

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  14. A Decrease in Mesophyll Conductance by Cell-Wall Thickening Contributes to Photosynthetic Downregulation(1) Reviewed

    Daisuke Sugiura, Ichiro Terashima, John R. Evans

    PLANT PHYSIOLOGY   Vol. 183 ( 4 ) page: 1600 - 1611   2020.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:AMER SOC PLANT BIOLOGISTS  

    It has been argued that accumulation of nonstructural carbohydrates triggers a decrease in Rubisco content, which downregulates photosynthesis. However, a decrease in the sink-source ratio in several plant species leads to a decrease in photosynthesis and increases in both structural and nonstructural carbohydrate content. Here, we tested whether increases in cell-wall materials, rather than starch content, impact directly on photosynthesis by decreasing mesophyll conductance. We measured various morphological, anatomical, and physiological traits in primary leaves of soybean (Glycine max) and French bean (Phaseolus vulgaris) grown under high- or low-nitrogen conditions. We removed other leaves 2 weeks after sowing to decrease the sink-source ratio and conducted measurements 0, 1, and 2 weeks after defoliation.Cell-wall thickening, rather than starch accumulation, leads to a decrease in mesophyll conductance after a reduction in the sink-source ratio in soybean and French bean.

    DOI: 10.1104/pp.20.00328

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  15. Nitrogen and Water Demands for Maximum Growth of Solanum tuberosum under Doubled CO2: Interaction with Phosphorus Based on the Demands Reviewed

    Yan Yi, Daisuke Sugiura, Katsuya Yano

    ENVIRONMENTAL AND EXPERIMENTAL BOTANY   Vol. 176   2020.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    Crop growth promotion utilizing elevated carbon dioxide concentrations (e[CO2]) may be limited by soil nutrient availability. Although numerous studies have suggested the importance of nitrogen (N) for the promotion of growth under e[CO2], N requirement for maximum plant growth is rarely examined. We have found that increase in potato (Solarium tuberosum L.) biomass depends on phosphorus (P) availability under doubled [CO2] conditions. To address whether the N requirement for maximum growth under e[CO2] is dependent on P supply or not in potatoes, we quantified potato growth and water consumption in response to five N supply rates at low P (LP) and high P (HP) conditions. A pot experiment was conducted in controlled-environment chambers with ambient CO2 concentrations (a[CO2]) and an e[CO2] level of double a[CO2]. Foliar critical N concentration per area (critical [N](area)), the minimum N requirement for 90% maximum plant growth, was similar (1.43 g m(-2)) regardless of [CO2] conditions under LP. Under HP, however, the critical [N](area) increased under e[CO2] conditions (1.65 g m(-2)) compared with a[CO2] conditions (1.52 g m(-2)). Water use did not change with e[CO2] under HP conditions, whereas it decreased with e[CO2] under LP conditions despite the increase in biomass owing to higher water-use efficiency (WUE). Although WUE with e[CO2] or HP was independent of N supply, biomass increment with e[CO2] or HP depended on N supply. We concluded that the N and water required by potato plants under e[CO2] would be dependent on P supply. Although under HP, e[CO2] increased N but not water required to obtain maximum growth during the early growth stage, N demand was unchanged and water demand decreased by e[CO2] under LP conditions, probably owing to growth limited by P availability.

    DOI: 10.1016/j.envexpbot.2020.104089

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  16. Comprehensive analysis of the mechanisms underlying enhanced growth and root N acquisition in rice by the endophytic diazotroph, Burkholderia vietnamiensis RS1 Reviewed

    Rina Shinjo, Aiko Tanaka, Daisuke Sugiura, Takamasa Suzuki, Kazuma Uesaka, Yumiko Takebayashi, Mikiko Kojima, Hitoshi Sakakibara, Daigo Takemoto, Motohiko Kondo

    PLANT AND SOIL   Vol. 450 ( 1-2 ) page: 537 - 555   2020.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:SPRINGER  

    Aims The aim of this study was to provide a comprehensive understanding of the mechanisms underlying response of rice (Oryza sativa L.) to the endophytic N-2-fixing bacterium Burkholderia vietnamiensis strain RS1 in growth and nitrogen (N) accumulation using transcriptome and hormonome analyses. Methods The effects of B. vietnamiensis RS1 on rice growth and root architecture were examined in pot and field conditions. To identify candidate plant genes that affect the growth of plants inoculated with B. vietnamiensis RS1, transcriptome analysis was conducted. We also examined the hormonal changes by hormonome analysis in relation to the morphological changes in roots. The contribution of N-2 fixation by B. vietnamiensis RS1 was estimated by the acetylene reduction activity (ARA). Results The inoculation of B. vietnamiensis RS1 resulted in increased shoot and root growth of rice plants accompanied by an increased root N absorption rate, especially under high N conditions. The contribution from N-2 fixation was minor because no effect of B. vietnamiensis RS1 on ARA was detected. Transcriptome analysis revealed an increase in the expression of genes related to the transportation and assimilation of N compounds, supporting the enhanced N absorption by roots. The expression of indole-3-acetic acid (IAA) signaling genes and concentrations of IAA increased, which may be related to the increased root development. Conclusions B. vietnamiensis RS1 enhanced rice growth by promoting N accumulation through enhanced root morphological development and N absorption ability; this outcome was probably related to the increased activities of N transportation and assimilation in the presence of a rich N supply.

    DOI: 10.1007/s11104-020-04506-3

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  17. Quantifying Phosphorus and Water Demand to Attain Maximum Growth of Solanum tuberosum in a CO2-Enriched Environment Reviewed

    Yan Yi, Daisuke Sugiura, Katsuya Yano

    FRONTIERS IN PLANT SCIENCE   Vol. 10   page: 1417   2019.11

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    Language:Japanese   Publishing type:Research paper (scientific journal)   Publisher:FRONTIERS MEDIA SA  

    Growth promotion by ambient CO2 enrichment may be advantageous for crop growth but this may be influenced by soil nutrient availability. Therefore, we quantified potato (Solanum tuberosum L.) growth responses to phosphorus (P) supply under ambient (a[CO2]) and elevated (doubled) CO2 concentration (e[CO2]). A pot experiment was conducted in controlled-environment chambers with a[CO2] and e[CO2] combined with six P supply rates. We obtained response curves of biomass against P supply rates under a[CO2] and e[CO2] (R-2 = 0.996 and R-2 = 0.992, respectively). A strong interaction between [CO2] and P was found. Overall, e[CO2] enhanced maximum biomass accumulation (1.5-fold) and water-use efficiency (WUE) (1.5-fold), but not total water use. To reach these maxima, minimum P supply rate at both [CO2] conditions was similar. Foliar critical P concentration (i.e., minimum [P] to reach 90% of maximum growth) was also similar at nearly 110 mg P m(-2). Doubling [CO2] did not increase P and water demand of potato plants, thus enabling the promotion of maximum growth without additional P or water supply, but via a significant increase in WUE (9.6 g biomass kg(-1) water transpired), presumably owing to the interaction between CO2 and P.

    DOI: 10.3389/fpls.2019.01417

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  18. Elevated CO2-induced changes in mesophyll conductance and anatomical traits in wild type and carbohydrate-metabolism mutants of Arabidopsis. Reviewed

    Mizokami Y, Sugiura D, Watanabe CKA, Betsuyaku E, Inada N, Terashima I

    Journal of experimental botany   Vol. 70 ( 18 ) page: 4807-4818   2019.9

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    DOI: 10.1093/jxb/erz208

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  19. How can we quantitatively study insects whose larvae live beneath the forest floor? A case study at an experimental long-term log-removal site in Japan Reviewed

    Tsunoda Tomonori, Hyodo Fujio, Sugiura Daisuke, Kaneko Nobuhiro, Suzuki Satoshi N.

    ENTOMOLOGICAL SCIENCE   Vol. 22 ( 3 ) page: 275-282   2019.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/ens.12362

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  20. QTL analysis for carbon assimilate translocation-related traits during maturity in rice (<i>Oryza sativa</i> L.). Reviewed

    Phung HD, Sugiura D, Sunohara H, Makihara D, Kondo M, Nishiuchi S, Doi K

    Breeding science   Vol. 69 ( 2 ) page: 289-296   2019.6

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    DOI: 10.1270/jsbbs.18203

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  21. Interspecific differences in how sink-source imbalance causes photosynthetic downregulation among three legume species Reviewed

    Sugiura Daisuke, Betsuyaku Eriko, Terashima Ichiro

    ANNALS OF BOTANY   Vol. 123 ( 4 ) page: 715-726   2019.3

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    DOI: 10.1093/aob/mcy204

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  22. Shoot nitrate underlies a perception of nitrogen satiety to trigger local and systemic signaling cascades in Arabidopsis thaliana Reviewed

    Okamoto Yuki, Suzuki Takamasa, Sugiura Daisuke, Kiba Takatoshi, Sakakibara Hitoshi, Hachiya Takushi

    SOIL SCIENCE AND PLANT NUTRITION   Vol. 65 ( 1 ) page: 56 - 64   2019.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1080/00380768.2018.1537643

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  23. Effects of elevated atmospheric CO2 on respiratory rates in mature leaves of two rice cultivars grown at a free-air CO2 enrichment site and analyses of the underlying mechanisms.

    Noguchi K, Tsunoda T, Miyagi A, Kawai-Yamada M, Sugiura D, Miyazawa SI, Tokida T, Usui Y, Nakamura H, Sakai H, Hasegawa T

    Plant & cell physiology     2018.2

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    DOI: 10.1093/pcp/pcy017

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  24. Sink-Source Balance and Down-Regulation of Photosynthesis in Raphanus sativus: Effects of Grafting, N and CO2

    Sugiura Daisuke, Watanabe Chihiro K. A., Betsuyaku Eriko, Terashima Ichiro

    PLANT AND CELL PHYSIOLOGY   Vol. 58 ( 12 ) page: 2043-2056   2017.12

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    DOI: 10.1093/pcp/pcx132

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  25. Arabidopsis Phosphatidic Acid Phosphohydrolases Are Essential for Growth under Nitrogen-Depleted Conditions

    Yoshitake Yushi, Sato Ryoichi, Madoka Yuka, Ikeda Keiko, Murakawa Masato, Suruga Ko, Sugiura Daisuke, Noguchi Ko, Ohta Hiroyuki, Shimojima Mie

    FRONTIERS IN PLANT SCIENCE   Vol. 8   2017.10

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    DOI: 10.3389/fpls.2017.01847

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  26. Enhanced leaf photosynthesis as a target to increase grain yield: insights from transgenic rice lines with variable Rieske FeS protein content in the cytochrome b6 /f complex. Reviewed

    Yamori W, Kondo E, Sugiura D, Terashima I, Suzuki Y, Makino A

    Plant, cell & environment   Vol. 39 ( 1 ) page: 80-7   2016.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/pce.12594

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  27. Phytohormonal Regulation of Biomass Allocation and Morphological and Physiological Traits of Leaves in Response to Environmental Changes in Polygonum cuspidatum. Reviewed

    Sugiura D, Kojima M, Sakakibara H

    Frontiers in plant science   Vol. 7   page: 1189   2016

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3389/fpls.2016.01189

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  28. Manipulation of the hypocotyl sink activity by reciprocal grafting of two Raphanus sativus varieties: its effects on morphological and physiological traits of source leaves and whole-plant growth Reviewed

    Daisuke Sugiura, Eriko Betsuyaku, Ichiro Terashima

    PLANT CELL AND ENVIRONMENT   Vol. 38 ( 12 ) page: 2629 - 40   2015.12

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:WILEY-BLACKWELL  

    To reveal whether hypocotyl sink activities are regulated by the aboveground parts, and whether physiology and morphology of source leaves are affected by the hypocotyl sink activities, we conducted grafting experiments using two Raphanus sativus varieties with different hypocotyl sink activities. Comet (C) and Leafy (L) varieties with high and low hypocotyl sink activities were reciprocally grafted and resultant plants were called by their scion and stock such as CC, LC, CL and LL. Growth, leaf mass per area (LMA), total non-structural carbohydrates (TNCs) and photosynthetic characteristics were compared among them. Comet hypocotyls in CC and LC grew well regardless of the scions, whereas Leafy hypocotyls in CL and LL did not. Relative growth rate was highest in LL and lowest in CC. Photosynthetic capacity was correlated with Rubisco (ribulose 15-bisphosphate carboxylase/oxygenase) content but unaffected by TNC. High C/N ratio and accumulation of TNC led to high LMA and structural LMA. These results showed that the hypocotyl sink activity was autonomously regulated by hypocotyl and that the down-regulation of photosynthesis was not induced by TNC. We conclude that the change in the sink activity alters whole-plant growth through the changes in both biomass allocation and leaf morphological characteristics in R. sativus.

    DOI: 10.1111/pce.12573

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  29. Roles of gibberellins and cytokinins in regulation of morphological and physiological traits in Polygonum cuspidatum responding to light and nitrogen availabilities

    Daisuke Sugiura, Koichiro Sawakami, Mikiko Kojima, Hitoshi Sakakibara, Ichiro Terashima, Masaki Tateno

    FUNCTIONAL PLANT BIOLOGY   Vol. 42 ( 4 ) page: 397 - 409   2015.4

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    Publishing type:Research paper (scientific journal)   Publisher:CSIRO PUBLISHING  

    We evaluated the roles of gibberellins (GAs) and cytokinins (CKs) in regulation of morphological traits such as biomass allocation and leaf mass per area (LMA). Seedlings of Polygonum cuspidatum Siebold & Zucc. were grown under various light and N availabilities. We exogenously sprayed solutions of gibberellin (GA(3)), benzyl adenine (BA), uniconazole (an inhibitor of GA biosynthesis) or their mixtures on the aboveground parts, and changes in morphological and physiological traits and relative growth rate (RGR) were analysed. Endogenous levels of GAs and CKs in the control plants were also quantified. The morphological traits were changed markedly by the spraying. Biomass allocation to leaves was increased by GA(3) and BA, whereas it decreased by uniconazole. GA(3) decreased LMA, whereas uniconazole increased it. We found close relationships among morphological and physiological traits such as photosynthetic rate and net assimilation rate, and RGR under all growth conditions. Seedlings with high levels of endogenous GAs or CKs and low levels of endogenous GAs or CKs showed morphologies similar to those sprayed with GA(3) or BA, and those sprayed with uniconazole, respectively. Thus we concluded these phytohormones are involved in the regulation of biomass allocation responding to either light or N availability.

    DOI: 10.1071/FP14212

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    PubMed

  30. Effects of the experimental alteration of fine roots on stomatal conductance and photosynthesis: Case study of devil maple (Acer diabolicum) in a cool temperate region

    Daisuke Sugiura, Masaki Tateno

    ENVIRONMENTAL AND EXPERIMENTAL BOTANY   Vol. 100   page: 105 - 113   2014.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    Water is absorbed by fine roots and transported with essential substances to conduct photosynthesis in leaves. To determine how many fine roots are required to absorb adequate water and maximise photosynthesis, the effects of decreasing fine root biomass on stomatal conductance (G(s)) and photosynthetic rate (A) were evaluated using theoretical simulations and laboratory experiments. We used saplings of devil maple (Acer diabolicum), a typical woody species in the cool temperate regions of Japan, grown in high- and low-light environments. A-G(s) relationships and whole-plant hydraulic conductance (K-w) were determined and used for the simulations. Gradual changes in K-w, A, and G(s) were also evaluated with a stepwise decrease in fine roots in the laboratory experiments. The model predicted that K-w, G(s), and A decreased moderately with a decrease in fine roots. For example, A decreased by only 12% when fine roots were decreased by 50%. The model predictions were nearly consistent with the results from laboratory experiments. In conclusion, saplings of devil maple produced fine roots that were more than sufficient in meeting the water demands of photosynthesis. These characteristics may be beneficial in surviving severe drought and in maintaining adequate hydraulic conductance under conditions of moderate water stress. (C) 2014 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.envexpbot.2013.12.020

    Web of Science

  31. High CO2 Triggers Preferential Root Growth of Arabidopsis thaliana Via Two Distinct Systems Under Low pH and Low N Stresses Reviewed

    Takushi Hachiya, Daisuke Sugiura, Mikiko Kojima, Shigeru Sato, Shuichi Yanagisawa, Hitoshi Sakakibara, Ichiro Terashima, Ko Noguchi

    PLANT AND CELL PHYSIOLOGY   Vol. 55 ( 2 ) page: 269 - 80   2014.2

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:OXFORD UNIV PRESS  

    Biomass allocation between shoots and roots is an important strategy used by plants to optimize growth in various environments. Root to shoot mass ratios typically increase in response to high CO2, a trend particularly evident under abiotic stress. We investigated this preferential root growth (PRG) in Arabidopsis thaliana plants cultivated under low pH/high CO2 or low nitrogen (N)/high CO2 conditions. Previous studies have suggested that changes in plant hormone, carbon (C) and N status may be related to PRG. We therefore examined the mechanisms underlying PRG by genetically modifying cytokinin (CK) levels, C and N status, and sugar signaling, performing sugar application experiments and determining primary metabolites, plant hormones and expression of related genes. Both low pH/high CO2 and low N/high CO2 stresses induced increases in lateral root (LR) number and led to high C/N ratios; however, under low pH/high CO2 conditions, large quantities of C were accumulated, whereas under low N/high CO2 conditions, N was severely depleted. Analyses of a CK-deficient mutant and a starchless mutant, in conjunction with sugar application experiments, revealed that these stresses induce PRG via different mechanisms. Metabolite and hormone profile analysis indicated that under low pH/high CO2 conditions, excess C accumulation may enhance LR number through the dual actions of increased auxin and decreased CKs.

    DOI: 10.1093/pcp/pcu001

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    PubMed

  32. Concentrative nitrogen allocation to sun-lit branches and the effects on whole-plant growth under heterogeneous light environments Reviewed

    Sugiura D

    Oecologia   Vol. 172 ( 4 ) page: 949-960   2013

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s00442-012-2558-7

    Scopus

  33. Long-term, short-interval measurements of the frequency distributions of the photosynthetically active photon flux density and net assimilation rate of leaves in a cool-temperate forest

    Ayana Miyashita, Daisuke Sugiura, Koichiro Sawakami, Ryuji Ichihashi, Tomokazu Tani, Masaki Tateno

    AGRICULTURAL AND FOREST METEOROLOGY   Vol. 152   page: 1 - 10   2012.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:ELSEVIER SCIENCE BV  

    Long-term, short-interval measurements of incident photosynthetically active photon flux density (PPFD; mu mol m(-2) s(-1)) on the forest floor are essential for estimating the leaf carbon gain of understory plants. Such PPFD data, however, are scarce. We measured PPFD at 1-min intervals for more than 12 months in cool-temperate forest sites and reported the data as a PPFD frequency distribution. We chose five sites: an open site (OPN), the understory of a deciduous broad-leaved tree stand with no visible gaps (DCD), that of an evergreen conifer stand (EVG), that of a deciduous broad-leaved tree stand with a gap of approximately 80 m(2) (GAPDCD), and that of an evergreen conifer stand with a gap of approximately 100 m(2) (GAPEVG). DCD were divided into three sub sites (DCD1-3) to investigate variation within a small area. GAP-sites were consisted of two sub sites (GAPDCD1-2 and GAPEVG1-2) differing in the distance from the gap center. Using the PPFD data, we estimated the summer seasonal (May-October) net assimilation rate of leaves (NAR(L)) at each site for various photosynthetic capacities (A(max): mu mol m(-2) s(-1)) and other parameters of a light response curve of CO2 assimilation rates. At OPN, the average daily accumulated PPFD (mol m(-2) day(-1)) was highest in May (28.2) and lowest in December (8.2). Even at OPN, the class of instantaneous PPFD that contributed most to NAR(L) was 250-300 mu mol m(-2) s(-1). Such a large contribution of lower PPFD is suggested to be an important feature of a field light-availability. At DCD, the relative PPFD (RPPFD, %) to OPN was 7.2 during canopy closure and 49.4 after leaf shedding (averaged for 3 sites). EVG had the lowest light environment throughout the year. Its average RPPFD was 3%. For GAP sites, summer seasonal RPPFD (%) was 15.6, 18.8, 6.4 and 15.6 for GAPDCD1, GAPDCD2, GAPEVG1 and GAPEVG2, respectively. At OPN, the NAR(L) increased with A(max) (which ranged from 1 to 40), suggesting that plants at OPN do not maximize NAR(L). In contrast, at DCD and EVG, A(max) values were attained that did maximize NAR(L), suggesting that plants at these sites could maximize the NAR(L). A(max)-NAR(L) relationships for GAPDCD and GAPEVG showed similar trend to closed canopy sites, DCD and EVG, while NAR(L) and A(max)* of GAP sites were larger than at these sites. Among DCD1-3, the daily accumulated PPFD (mol m-2 day-1) averaged in summer ranged 1.3-1.8 and the maximum NAR(L) value differed up to 1.5 times. It indicates that A(max) and NAR(L) can be various among plants under a similar canopy conditions. (C) 2011 Elsevier B.V. All rights reserved.

    DOI: 10.1016/j.agrformet.2011.08.001

    Web of Science

  34. Optimal Leaf-to-Root Ratio and Leaf Nitrogen Content Determined by Light and Nitrogen Availabilities Reviewed

    Daisuke Sugiura, Masaki Tateno

    PLOS ONE   Vol. 6 ( 7 ) page: e22236   2011

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:PUBLIC LIBRARY SCIENCE  

    Plants exhibit higher leaf-to-root ratios (L/R) and lower leaf nitrogen content (N(area)) in low-light than in high-light environments, but an ecological significance of this trait has not been explained from a whole-plant perspective. This study aimed to theoretically and experimentally demonstrate whether these observed L/R and N(area) are explained as optimal biomass allocation that maximize whole-plant relative growth rate (RGR). We developed a model which predicts optimal L/R and N(area) in response to nitrogen and light availability. In the model, net assimilation rate (NAR) was determined by light-photosynthesis curve, light availability measured during experiments, and leaf temperature affecting the photosynthesis and leaf dark respiration rate in high and low-light environments. Two pioneer trees, Morus bombycis and Acer buergerianum, were grown in various light and nitrogen availabilities in an experimental garden and used for parameterizing and testing the model predictions. They were grouped into four treatment groups (relative photosynthetic photon flux density, RPPFD 100% or 10% x nitrogen-rich or nitrogen-poor conditions) and grown in an experimental garden for 60 to 100 days. The model predicted that optimal L/R is higher and N(area) is lower in low-light than high-light environments when compared in the same soil nitrogen availability. Observed L/R and N(area) of the two pioneer trees were close to the predicted optimums. From the model predictions and pot experiments, we conclude that the pioneer trees, M. bombycis and A. buergerianum, regulated L/R and N(area) to maximize RGR in response to nitrogen and light availability.

    DOI: 10.1371/journal.pone.0022236

    Web of Science

    PubMed

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Presentations 15

  1. Nitrogen and Water Demands for Maximum Growth of <i>Solanum tuberosum</i> under doubled CO<sub>2</sub>: Interaction with Phosphorus Based on the Demands

    Yi Yan, Sugiura Daisuke, Yano Katsuya

    Abstracts of Meeting of the CSSJ  2020.3.24  CROP SCIENCE SOCIETY OF JAPAN

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    Language:English   Presentation type:Oral presentation (general)  

    DOI: 10.14829/jcsproc.249.0_131

    CiNii Research

  2. Sago Palm for Starch Production and Sustainable Land Development in Peatlands

    Azhar Aidil, Asano Koki, Audia Fitri, Naito Hitoshi, Sugiura Daisuke, Kano-Nakata Mana, Makihara Daigo, Ehara Hiroshi

    Abstracts of Meeting of the CSSJ  2021.3.29  CROP SCIENCE SOCIETY OF JAPAN

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    Language:English   Presentation type:Oral presentation (general)  

    DOI: 10.14829/jcsproc.251.0_166

    CiNii Research

  3. Comparison of methods for calculating mesophyll and chloroplast surface areas facing to intercellular airspaces based on 3D reconstruction models and 2D section images

    Ouk Rachana, Oi Takao, Sugiura Daisuke, Taniguchi Mitsutaka

    Abstracts of Meeting of the CSSJ  2021.3.29  CROP SCIENCE SOCIETY OF JAPAN

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    Language:English   Presentation type:Oral presentation (general)  

    DOI: 10.14829/jcsproc.251.0_112

    CiNii Research

  4. 気孔の微気象応答メカニズムから迫るC3・C4作物の水利用戦略

    杉浦大輔、尾関健吾

    日本植物学会第84回大会  2020.9.19 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

  5. ダイズ葉に蓄積した非構造性炭水化物は気孔開口速度を低下させる"

    小澤佑依, 田中愛子, 鈴木孝征, 杉浦大輔

    日本植物学会第84回大会  2020.9.19 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

  6. 4-1-7 Nitrogen and Phosphorus Demands for Maximum Growth of <i>Solanum tuberosum</i> under Doubled CO<sub>2</sub>(4-1 植物の多量栄養素 2020年度岡山大会)

    易 燕, 杉浦 大輔, 矢野 勝也

    日本土壌肥料学会講演要旨集  2020  一般社団法人 日本土壌肥料学会

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    Presentation type:Oral presentation (general)  

  7. Elucidation of varietal and regional differences in rice canopy growth to micro climate applying non-destructive LAI measurement method

    Fukuda Shota, Hosoi Jun, Sugiura Daisuke

    Abstracts of Meeting of the CSSJ  2019  CROP SCIENCE SOCIETY OF JAPAN

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    Presentation type:Oral presentation (general)  

  8. Bacterial flora of nitrogen-fixing endophytes in stem of rice genotypes with different non-structural carbohydrates accumulation

    Okamoto Takanori, Shinjo Rina, Nishihara Arisa, Uesaka Kazuma, Tanaka Aiko, Sugiura Daisuke, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2020  CROP SCIENCE SOCIETY OF JAPAN

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    Presentation type:Oral presentation (general)  

  9. Varietal difference in the response to reductive rhizosphere environment in rice

    Hiramatsu Shuichi, Sugiura Daisuke, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2018  CROP SCIENCE SOCIETY OF JAPAN

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    Presentation type:Oral presentation (general)  

  10. Development of non-destructive LAI measuring technique using NIR/PAR ratio to evaluate rice canopy growth

    Sugiura Daisuke, Fukuda Shota, Hosoi Jun, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2019  CROP SCIENCE SOCIETY OF JAPAN

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    Presentation type:Oral presentation (general)  

  11. Evaluation of water use characteristics in wheat and finger millet under fluctuating light

    Ozeki Kengo, Daisuke Sugiura

    Abstracts of Meeting of the CSSJ  2020  CROP SCIENCE SOCIETY OF JAPAN

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    Presentation type:Oral presentation (general)  

  12. Varietal difference in the response to soil redox status in rice

    Hiramatsu Shuichi, Sugiura Daisuke, Nagano Atsushi J., Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2020  CROP SCIENCE SOCIETY OF JAPAN

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    Presentation type:Oral presentation (general)  

  13. Eco-physiological significance of down- and up-regulation of photosynthesis in soybean and French bean

    Sugiura Daisuke, Kono Masaru, Yamori Wataru, Terashima Ichiro

    Abstracts of Meeting of the CSSJ  2017  CROP SCIENCE SOCIETY OF JAPAN

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    Presentation type:Oral presentation (general)  

  14. Relationship between Endophytic nitrogen fixing ability and Non-structural carbohydrate in rice varieties

    Okamoto Takanori, Shinjo Rina, Tanaka Aiko, Sugiura Daisuke, Kondo Motohiko

    Abstracts of Meeting of the CSSJ  2018  CROP SCIENCE SOCIETY OF JAPAN

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    Presentation type:Oral presentation (general)  

  15. イネ<i>plr1</i>変異はデンプン・糖蓄積パターンの変化を通して側根発育を制御する

    Lucob-Agustin Nonawin, 杉浦 大輔, 仲田(狩野) 麻奈, 長谷川 友美, 井成(池田) 真由子, 山内 章, 犬飼 義明

    日本作物学会講演会要旨集  2020  日本作物学会

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KAKENHI (Grants-in-Aid for Scientific Research) 6

  1. イネ群落成長の非破壊連続測定・微気象応答解析を通じた多収品種の収量制限要因の解明

    Grant number:20H02965  2020.4 - 2023.3

    杉浦 大輔

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    Authorship:Principal investigator 

    Grant amount:\17940000 ( Direct Cost: \13800000 、 Indirect Cost:\4140000 )

    イネ群落の様々な分光反射特性から、群落成長および生理特性の日変化を連続・非破壊的に測定する手法を開発し、様々な品種特性の微気象応答性から収量決定要因までを、定量的に解明することを目的とする。現在の気候条件下における現行イネ品種の栽培適性地や収量ポテンシャルの評価、将来の気候変動環境下でより高成長・収量を示すイネ品種デザインの提示といった、データ駆動型の育種・品種選定が可能になることが期待される。

  2. Physiological mechanisms of carbohydrate utilization by N fixing endophyte for improving N fixation capacity in rice

    Grant number:19H02941  2019.4 - 2023.3

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    Authorship:Coinvestigator(s) 

  3. Screening mutant lines showing enhanced water use efficiencies with a newly developed system for its visualization

    Grant number:18KK0170  2018.10 - 2022.3

    Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

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    Authorship:Coinvestigator(s) 

  4. Responses of gene expressions in rice plants to micro-meteorological conditions; their physiological role in absorption of water and nutrients from roots

    Grant number:17H03896  2017.4 - 2020.3

    KUWAGATA Tsuneo

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    Authorship:Coinvestigator(s) 

    We experimentally investigated how micro-meteorological conditions influence expression levels of the functional genes which contribute to absorption of water and nutrients by the rice crops, and examined the physiological roles of the environmental responses of these genes to the growth of rice. The experimental results indicated that the expression levels of many kinds of aquaporin genes both in roots and leaves increased with the transpirational demand during the all growth stages, and that expression level of about half of all genes in the rice leaves had a positive (or negative) correlation with transpiration in the early ripening period. Finally, we demonstrated that the environmental response of functional genes to day-to-day variation in micro-meteorological conditions could play important roles in several eco-physiological processes, such as physiological response of roots to decrease in soil water content and change in water use efficiency with atmospheric CO2 concentration.

  5. Investigation of interspecific differences in regulatory mechanism of photosynthesis by spectroscopic imaging and microclimate analysis

    Grant number:17K15192  2017.4 - 2020.3

    Sugiura Daisuke

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    Authorship:Principal investigator 

    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

    This study aimed at elucidating responses of photosynthetic characteristics and canopy growth to micro-climate and their interspecific or intercultivar differences in crops. Physiological mechanisms of down-regulation of photosynthesis responding to non-structural carbohydrates in soybean and French bean were closely examined. We also developed a technique to estimate rice canopy growth non-destructively by measuring near infra-red radiation (NIR) and photosynthetically active radiation (PAR) inside and outside rice canopy. This technique allowed us to trace seasonal dynamics of canopy growth from transplanting to maturity stage and to evaluate varietal and regional differences in the canopy growth characteristics.

  6. 植物の個体レベルのC・N分配調節メカニズムに関する生理生態学的研究

    Grant number:25891011  2013.8 - 2015.3

    杉浦 大輔

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    Authorship:Principal investigator 

    Grant amount:\2730000 ( Direct Cost: \2100000 、 Indirect Cost:\630000 )

    植物は個体レベルの葉、茎、根への炭水化物 (C) や窒素 (N) の分配や、葉の面積あたりの重さ (LMA) を、環境条件に応じて変化させる。本研究では、このようなC・N分配パターンや、LMAを調節するメカニズムについて、植物ホルモンであるジベレリン (GA) やサイトカイニン (CK) の役割や、シンクソースバランスに応じて葉に蓄積する糖が果たす役割を解明することを目的として行った。
    (1) 草本植物イタドリ (Polygonum cuspidatum) を用いて、光やN条件の変化に応じた個体レベルのC分配の変化と、内生GAやCKの変化の関係を解析した。強光・弱光の各条件下では、高N条件ほど、地上部全体のCK濃度が高く、同時に個体レベルの葉へのC分配比も高かった。活性型GA濃度も同様に高N条件ほど高く、強光・低N条件ではほとんど検出されなかった。外生GA、CK添加時に見られる葉へのC分配比と、内生GA、CK濃度が高いときに見られる葉へのC分配比は同様の傾向を示すため、これらの植物ホルモンが個体レベルのC分配の変化を調節するメカニズムに深く関わっていることが示唆された。
    (2) 草本植物ダイコン (Raphanus sativus) の、胚軸が肥大する品種 (コメット:貯蔵シンク活性が高い) と肥大しない品種 (葉大根:活性が低い) を接ぎ木することでシンクソースバランスを変化させ、葉へ蓄積する糖がLMAや葉の構造に与える影響を評価した。どちらの品種でも、貯蔵シンク活性が低い葉大根の胚軸を台にすると、シンク活性が高いコメットを台にした時と比べ、LMAが大幅に増加した。増加分はデンプンだけでは説明できず、葉の厚さはほとんど変化していなかったため、デンプンが蓄積するほど細胞壁成分が増加している可能性が示された。葉の横断切片の構造解析を行ったところ、細胞数や細胞密度がデンプン量に応じて変化していることが分かった。
    翌年度、交付申請を辞退するため、記入しない。
    翌年度、交付申請を辞退するため、記入しない。

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