Updated on 2022/04/15

写真a

 
KITO Seiji
 
Organization
Administrative Support Organizations Animal Research Support Center Professor
Title
Professor

Degree 3

  1. Doctor of Veterinary Science ( 1996.8   University of Wisconsin-Madison ) 

  2. Master of Science ( 1991.9   Kyoto University ) 

  3. Bachelor of Science ( 1989.3   Kyoto University ) 

Research Interests 3

  1. preimplantation embryo, gamete

  2. Fertilization

  3. Assisted reproductive technology

Research Areas 2

  1. Life Science / Laboratory animal science

  2. Life Science / Animal production science

Research History 6

  1. Nagoya University   Administrative Support Organizations Center for Animal Research and Education   Professor

    2017.4

      More details

    Country:Japan

  2. Nagoya University   Administrative Support Organizations Animal Research Support Center   Manager of Higashiyama Laboratory Animal Facility

    2017.4

  3. The National Institutes for Quantum and Radiological Science and Technology   Department of Information Technology, Research Information Section   Chief Researcher

    2016.7 - 2017.3

      More details

    Country:Japan

  4. MEXT   Research Promotion Bereau   Administrative Researcher

    2010.4 - 2011.3

      More details

    Country:Japan

  5. National Institute of Radiological Sciences   Department of Engineering and Safety, Laboratory Animal and Genome Sciences Section   Researcher

    1998.4 - 2016.6

      More details

    Country:Japan

  6. Cornell University   Department of Physiology   Post-Doctoral Research Associate

    1996.9 - 1998.2

      More details

    Country:United States

▼display all

Education 3

  1. University of Wisconsin-Madison   Department of Animal Health and Biomedical Science   Reproductive Physiology

    1991.9 - 1996.8

      More details

    Country: United States

  2. Kyoto University   Graduate School, Division of Agriculture   Department of Animal Science

    1989.4 - 1991.9

      More details

    Country: Japan

  3. Kyoto University   Faculty of Agriculture   Department of Animal Science

    1984.4 - 1989.3

      More details

    Country: Japan

Professional Memberships 6

  1. Japan Association for Laboratory Animal Science

    2018.4

  2. Japan Society for Ova Reseach

  3. Society for the Study of Reproduction

  4. The Society for Reproduction and Development

  5. The Japanese Association for Experimental Animal Technologists

  6. The Japanese Association for Experimental Animal Technologists

▼display all

Committee Memberships 1

  1. 国立大学法人動物実験施設協議会   組織委員会委員  

    2018.11 - 2020.4   

      More details

    Committee type:Academic society

 

Papers 28

  1. Synergistic Effects of Chronic Restraint-Induced Stress and Low-Dose 56Fe-particle Irradiation on Induction of Chromosomal Aberrations in Trp53-Heterozygous Mice. Reviewed International journal

    Katsube T, Wang B, Tanaka K, Ninomiya Y, Hirakawa H, Liu C, Maruyama K, Vares G, Liu Q, Kito S, Nakajima T, Fujimori A, Nenoi M

    Radiation research     2021.4

     More details

    Authorship:Lead author, Last author, Corresponding author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1667/RADE-20-00218.1

    PubMed

  2. Detection of Alzheimer's disease-related neuroinflammation by a PET ligand selective for glial versus vascular translocator protein. Reviewed International journal

    Ji B, Ono M, Yamasaki T, Fujinaga M, Zhang MR, Seki C, Aoki I, Kito S, Sawada M, Suhara T, Sahara N, Higuchi M

    Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     page: 271678X21992457   2021.2

     More details

    Authorship:Lead author, Last author, Corresponding author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0271678X21992457

    PubMed

  3. Development of Human Sodium/Iodide Symporter (hNIS) Transgenic Mouse as Resource of Stem Cells for in vivo Cell Tracking Reviewed International journal

    Inubushi M., Michikawa Y., Kito S., Takeuchi Y., Kitagawa Y., Saga T.

    EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING   Vol. 45   page: S624 - S625   2018.10

     More details

    Authorship:Lead author, Last author, Corresponding author   Publishing type:Research paper (scientific journal)  

    Web of Science

  4. Regulation of Anxiety and Depression by Mitochondrial Translocator Protein-Mediated Steroidogenesis: the Role of Neurons Reviewed

    Barron AM, Higuchi M, Hattori S, Kito S, Suhara T, Ji B

    Molecular neurobiology   Vol. 58 ( 2 ) page: 550 - 563   2021.2

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s12035-020-02136-5

    Other Link: https://link.springer.com/article/10.1007/s12035-020-02136-5

  5. Digenic mutations in ALDH2 and ADH5 impair formaldehyde clearance and cause a multisystem disorder, AMeD syndrome Reviewed

    Oka Yasuyoshi, Hamada Motoharu, Nakazawa Yuka, Muramatsu Hideki, Okuno Yusuke, Higasa Koichiro, Shimada Mayuko, Takeshima Honoka, Hanada Katsuhiro, Hirano Taichi, Kawakita Toshiro, Sakaguchi Hirotoshi, Ichimura Takuya, Ozono Shuichi, Yuge Kotaro, Watanabe Yoriko, Kotani Yuko, Yamane Mutsumi, Kasugai Yumiko, Tanaka Miyako, Suganami Takayoshi, Nakada Shinichiro, Mitsutake Norisato, Hara Yuichiro, Kato Kohji, Mizuno Seiji, Miyake Noriko, Kawai Yosuke, Tokunaga Katsushi, Nagasaki Masao, Kito Seiji, Isoyama Keiichi, Onodera Masafumi, Kaneko Hideo, Matsumoto Naomichi, Matsuda Fumihiko, Matsuo Keitaro, Takahashi Yoshiyuki, Mashimo Tomoji, Kojima Seiji, Ogi Tomoo

    SCIENCE ADVANCES   Vol. 6 ( 51 )   2020.12

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.1126/sciadv.abd7197

    Web of Science

  6. Effects of high calcium levels on the disturbed extrusion of the second polar body duringin vitrofertilization in C3H/He mouse substrains Reviewed

    Ohta-Takada Y., Nagao Y., Kito S.

    ZYGOTE   Vol. 28 ( 1 ) page: 83 - 85   2020.2

     More details

    Publishing type:Research paper (scientific journal)  

    DOI: 10.1017/S0967199419000662

    Web of Science

  7. Effects of high calcium levels on the disturbed extrusion of the second polar body during in vitro fertilization in C3H/He mouse substrains Reviewed

    Y. Ohta-Takada, Y. Nagao and S. Kito

    Zygote     2019.10

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: : 10.1017/S096719941900066

    PubMed

  8. Steroidogenic abnormalities in translocator protein knockout mice and significance in the aging male. Reviewed

    Barron AM, Ji B, Kito S, Suhara T, Higuchi M.

    Biochemical Journal   Vol. 475 ( 1 ) page: 75-85   2018.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1042/BCJ20170645

    PubMed

  9. Multimodal Imaging for DREADD-Expressing Neurons in Living Brain and Their Application to Implantation of iPSC-Derived Neural Progenitors. Reviewed

    Ji B, Kaneko H, Minamimoto T, Inoue H, Takeuchi H, Kumata K, Zhang MR, Aoki I, Seki C, Ono M, Tokunaga M, Tsukamoto S, Tanabe K, Shin RM, Minamihisamatsu T, Kito S, Richmond BJ, Suhara T, Higuchi M

    The Journal of neuroscience : the official journal of the Society for Neuroscience   Vol. 36 ( 45 ) page: 11544-11558   2016.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1523/JNEUROSCI.1279-16.2016

    PubMed

  10. Deficiencies in extrusion of the second polar body due to high calcium concentrations during in vitro fertilization in inbred C3H/He mice. Reviewed

    Ohta Y, Nagao Y, Minami N, Tsukamoto S, Kito S.

    Zygote.   Vol. 24 ( 4 ) page: 603-616   2016.8

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1017/S096719941500060X

    PubMed

  11. Ionizing radiation, inflammation, and their interactions in colon carcinogenesis in Mlh1-deficient mice. Reviewed

    Morioka T, Miyoshi-Imamura T, Blyth BJ, Kaminishi M, Kokubo T, Nishimura M, Kito S, Tokairin Y, Tani S, Murakami-Murofushi K, Yoshimi N, Shimada Y, Kakinuma S

    Cancer science   Vol. 106 ( 3 ) page: 217-26   2015.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/cas.12591

    PubMed

  12. Fluorescence-based visualization of autophagic activity predicts mouse embryo viability. Reviewed

    Tsukamoto S, Hara T, Yamamoto A, Kito S, Minami N, Kubota T, Sato K, Kokubo T

    Scientific reports   Vol. 4   page: 4533   2014.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/srep04533

    PubMed

  13. Disruption of Aspm causes microcephaly with abnormal neuronal differentiation Reviewed

    Fujimori A, Itoh K, Goto S, Hirakawa H, Wang B, Kokubo T, Kito S, Tsukamoto S, Fushiki S.

    Brain and Development   Vol. 36 ( 8 ) page: 661-669   2013.11

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.braindev.2013.10.006

    PubMed

  14. The promoter of the oocyte-specific gene, Oog1, functions in both male and female meiotic germ cells in transgenic mice. Reviewed

    Ishida M, Okazaki E, Tsukamoto S, Kimura K, Aizawa A, Kito S, Imai H, Minami N

    PloS one   Vol. 8 ( 7 ) page: e68686   2013

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0068686

    PubMed

  15. High osmolality vitrification: a new method for the simple and temperature-permissive cryopreservation of mouse embryos. Reviewed

    Mochida K, Hasegawa A, Li MW, Fray MD, Kito S, Vallelunga JM, Lloyd KC, Yoshiki A, Obata Y, Ogura A

    PloS one   Vol. 8 ( 1 ) page: e49316   2013

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0049316

    PubMed

  16. Genomic and gene expression signatures of radiation in medulloblastomas after low-dose irradiation in Ptch1 heterozygous mice. Reviewed

    Ishida Y, Takabatake T, Kakinuma S, Doi K, Yamauchi K, Kaminishi M, Kito S, Ohta Y, Amasaki Y, Moritake H, Kokubo T, Nishimura M, Nishikawa T, Hino O, Shimada Y

    Carcinogenesis   Vol. 31 ( 9 ) page: 1694-701   2010.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/carcin/bgq145

    PubMed

  17. Superovulatory response, oocyte spontaneous activation, and embryo development in WMN/Nrs inbred rats. Reviewed

    Kito S, Yano H, Ohta Y, Tsukamoto S

    Experimental animals   Vol. 59 ( 1 ) page: 35-45   2010

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PubMed

  18. In vitro fertilization in inbred BALB/c mice II: effects of lactate, osmolarity and calcium on in vitro capacitation. Reviewed

    Kito S, Ohta Y

    Zygote (Cambridge, England)   Vol. 16 ( 3 ) page: 259-70   2008.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1017/S0967199408004619

    PubMed

  19. In vitro fertilization in inbred BALB/c mice I: isotonic osmolarity and increased calcium-enhanced sperm penetration through the zona pellucida and male pronuclear formation. Reviewed

    Kito S, Ohta Y

    Zygote (Cambridge, England)   Vol. 16 ( 3 ) page: 249-57   2008.8

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1017/S0967199408004607

    PubMed

  20. Differential effects of low- and high-dose X-rays on N-ethyl-N-nitrosourea-induced mutagenesis in thymocytes of B6C3F1 gpt-delta mice. Reviewed

    Yamauchi K, Kakinuma S, Sudo S, Kito S, Ohta Y, Nohmi T, Masumura K, Nishimura M, Shimada Y

    Mutation research   Vol. 640 ( 1-2 ) page: 27-37   2008.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.mrfmmm.2007.12.001

    PubMed

  21. Developmental responses of 2-cell embryos to oxygen tension and bovine serum albumin in Wistar rats. Reviewed

    Kito S, Kaneko Y, Yano H, Tateno S, Ohta Y

    Experimental animals   Vol. 57 ( 2 ) page: 123-8   2008.4

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PubMed

  22. Medium effects on capacitation and sperm penetration through the zona pellucida in inbred BALB/c spermatozoa. Reviewed

    Kito S, Ohta Y

    Zygote (Cambridge, England)   Vol. 13 ( 2 ) page: 145-53   2005.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PubMed

  23. Severe growth retardation and short life span of double-mutant mice lacking Xpa and exon 15 of Xpg. Reviewed

    Shiomi N, Mori M, Kito S, Harada YN, Tanaka K, Shiomi T

    DNA repair   Vol. 4 ( 3 ) page: 351-7   2005.3

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.dnarep.2004.10.009

    PubMed

  24. Improved in vitro fertilization and development by use of modified human tubal fluid and applicability of pronucleate embryos for cryopreservation by rapid freezing in inbred mice. Reviewed

    Kito S, Hayao T, Noguchi-Kawasaki Y, Ohta Y, Hideki U, Tateno S

    Comparative medicine   Vol. 54 ( 5 ) page: 564-70   2004.10

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PubMed

  25. Identification of the XPG region that causes the onset of Cockayne syndrome by using Xpg mutant mice generated by the cDNA-mediated knock-in method. Reviewed

    Shiomi N, Kito S, Oyama M, Matsunaga T, Harada YN, Ikawa M, Okabe M, Shiomi T.

    Molecular and cellular Biology   Vol. 24 ( 9 ) page: 3712-9   2004.5

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1128/MCB.24.9.3712-3719.2004

    PubMed

  26. Growth retardation and skin abnormalities of the Recql4-deficient mouse. Reviewed

    Hoki Y, Araki R, Fujimori A, Ohhata T, Koseki H, Fukumura R, Nakamura M, Takahashi H, Noda Y, Kito S, Abe M

    Human molecular genetics   Vol. 12 ( 18 ) page: 2293-9   2003.9

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/hmg/ddg254

    PubMed

  27. Evaluation of developmental competence of vitrified-warmed early cleavage stage embryos and their application for chimeric mouse production. Reviewed

    Kito S, Noguchi Y, Ohta Y, Ohhata T, Abe M, Shiomi N, Shiomi T.

    Experimental Animals   Vol. 52 ( 2 ) page: 179-83   2003.3

     More details

    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1538/expanim.52.179

    PubMed

  28. Developmental responses of two substrains of in vitro fertilized C57BL/6J mouse embryos to oxygen and amino acids. Reviewed

    Kito S, Noguchi Y, Ohta Y

    Experimental animals   Vol. 52 ( 1 ) page: 63-6   2003.1

     More details

    Language:English   Publishing type:Research paper (scientific journal)  

    PubMed

▼display all

MISC 1

  1. Functional analysis of lysosomes during mouse preimplantation embryo development. Reviewed

    Tsukamoto S, Hara T, Yamamoto A, Ohta Y, Wada A, Ishida Y, Kito S, Nishikawa T, Minami N, Sato K, Kokubo T

    The Journal of reproduction and development   Vol. 59 ( 1 ) page: 33-9   2013

     More details

    Language:English  

    PubMed

Presentations 1

  1. 配偶子・初期胚培養液の開発 -黎明期、暗黒時代、ルネッサンス、そして現在の安定期まで- Invited

    鬼頭 靖司

    実験動物技術者協会 関東支部 REG部会 第20回特別講演会 

     More details

    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:慶應義塾大学医学部   Country:Japan  

    本講演では、今は一般的に市販品として入手可能な初期胚培養用の培養液がどのような経緯を経て開発されてきたかについて主にげっ歯類を中心に紹介する。

KAKENHI (Grants-in-Aid for Scientific Research) 6

  1. Molecular Imaging of Transplanted Stem Cells Derived from Original NIS Transgenic Mouse

    Grant number:26293282  2014.4 - 2018.3

    INUBUSHI Masayuki

      More details

    Authorship:Coinvestigator(s) 

    Using a transgenic mouse systemically and stabley expressing NIS genes (NIS-Tg mouse), we collaborated on the following 3 studies with specialists. (1) We succeeded in changing over its breeding from hetero-crossing to homo-crossing that increases possibility for higher gene expression, and almost established its inbred line (Kawasaki Med School). (2) We succeeded in in-vivo quantitative analysis of the function of stem-cell sheets made with stem cells derived from the NIS-Tg mouse, which contributed to the development of a novel iPS-sheet therapy for myocardial infarction in humans (NCVC). (3) We performed transplantation of stem cells derived from the NIS-Tg mouse to irradiated mice, and investigated how and with which specific character of stem cells the mice could be survived after high-dose radiation exposure (NIRS).

  2. Development of hereditary non-polyposis colon cancer model using the Mlh1-deficient heterozygous mice

    Grant number:22501002  2010 - 2012

    KAKINUMA Shizuko

      More details

    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid) 

    Heterozygous germline mutations of mismatch repair (MMR) genes cause human hereditary non-polyposis colon cancer, and homozygous mutations are manifested by early onset of childhood T- or B-cell leukemia. In order to develop models for both colon cancer and lymphoma, we examined induction protocols with radiation and an inflammatory agent in Mlh1-/- homozygous and Mlh1+/- heterozygous mice. The protocols we established reproducibly induced colon cancer in Mlh1-/- homozygous and Mlh1+/- heterozygous mice, and lymphoma in Mlh1-/- homozygous mice using radiation and/or an inflammatory agent.

  3. 精子形成期の半数体特異的遺伝子発現を利用した単性動物の作出

    Grant number:21658094  2009 - 2010

    南 直治郎

      More details

    Authorship:Coinvestigator(s) 

    本研究においては、雌雄の産み分けが可能な雄を作出する目的で、22年度は下記の2点について検討を行った。
    (1) 導入遺伝子産物であるmRNAおよび翻訳後のタンパク質が精子形成期の細胞間架橋を自由に通過できないような仕組みを開発する。
    (2) 遺伝子導入された染色体を持つ精子が受精できない仕組みを開発する。
    (1)についてはSmok1遺伝子の5'および3'非翻訳領域が細胞間架橋に関与することが明らかになったが、その正確な遺伝子配列は公表されていない。そこで、それぞれの配列のクローニングを行い、実際にゲノム上およびcDNA中にその配列が存在することを確認し、遺伝子組換えマウス作製のための組換えベクターを作出した。(2)については精子アクロソーム膜上に発現し、受精に関与することが示唆されているタンパク質をコードする遺伝子にRSK3遺伝子の3'UTRを接続した融合遺伝子を構築し、この遺伝子をプロタミン制御下で発現する遺伝子組換えマウスを作製した。作出したマウスのF1個体を使って、受精に及ぼす影響および細胞間架橋の通過について検討した。いくつかの系統では正常に産子が得られたため、受精には影響していないと考えられたが、不妊傾向が認められる系統も得られており、現在解析を行っている。

  4. Analysis of oocyte-specific gene, Oog1 and its involvement in molecular basis of zygotic gene activation

    Grant number:19380158  2007 - 2009

    MINAMI Naojiro

      More details

    Authorship:Coinvestigator(s) 

    In the present study, we investigated the function of oocyte-specific gene, Oog1. The gene translocates to the nucleus of fertilized embryos at the time of zygotic gene activation. In order to analyze the function of the gene, we produced the transgenic mice in which the mRNA of Oog1 is knockdown. In addition, we investigated the protein expression of Oog1 during female meiosis and it is revealed that the protein exists in the oocyte nucleus at the prophase of meiosis. In another experiments, we identified the regulatory regions of Oog1 which controls the expression of the gene.

  5. Development of the gene deletion model rats by random mutagenesis.

    Grant number:16500282  2004 - 2005

    HARADA Yoshinobu

      More details

    Authorship:Coinvestigator(s) 

    Random mutagenesis with chlorambucil was performed in order to produce some new mutant rats. To identify the optimal quantity of chlorambucil and the optimal mating time for mutagenesis, we counted the number of new born rats born from female rats mated with the male rat medicated with several dose of chlorambucil. Consequently, it became clear that between 2.5mg and 15mg per kg weight of chlorambucil are good for medication, and it is good to perform mating between three weeks and six weeks after the medication of chlorambucil. We obtained several dwarfish rats from BN and F344 strans. Dwarfish rats whose sizes of the body were about 30% of normal rats were also observed. We also obtained a mutant rat with fiblosarcoma from the BN strain. This fiblosarcoma has grown up to 5cm or more in the diameter within 2 weeks. Unfortunately, we have not determined the genome region of delition of this rat by quontitative PCR analysis. Future experiments will focus on searching this genome region of delition by microarray or BAC array analyses.

  6. Development of the gene deletion model rats by random mutagenesis

    Grant number:14580805  2002 - 2003

    HARADA Yoshinobu

      More details

    Authorship:Coinvestigator(s) 

    Random mutagenesis with chlorambucil was performed in order to produce some new mutant rats. To identify the optimal quantity of chlorambucil and the optimal mating time for mutagenesis, we counted the number of new born rats born from female rats mated with the male rat medicated with several dose of chlorambucil. Consequently, it became clear that between 5mg and 15mg per kg weight of chlorambucil are good for medication, and it is good to perform mating between three weeks and six weeks after the medication of chlorambucil. The female (G1 generation) rat was obtained as a result of crossing between the male rat of BN strain medicated with 10mg chlorambucil and a normal F344 female rat. This female rat has the capability to conceive. A male rat (G3 generation) was obtained from a cross the female rat (G2 generation) with the male rat (G3 generation). Then we crossed these rats and obtained six rats. When these six rats were bred for about one year, one of the rats had skin cancer and another one showed diabetes symptom. It was presumed that these rats are the mutants by which mutation was occurred in some genes. In addition, the dwarfish rat whose size of the body is about 30% of a normal rat was also observed. In almost all cases, the dwarfish rats died before weaning, but a few dwarfish rats grew to the adult. Although the adult dwarfish rats was able to be conceived, they born some dwarfish rats by mating with a normal rat similarly. In order to confirm to which gene mutation has happened, gene expression analysis was performed for the G5 generation rats using DNA microarray. Consequently, it was proved that at least ten kinds of genes was not expressed in the G5 rats. These genes were presumed that the expression of these genes was suppressed by deletion type of mutation in each genes.

▼display all