Updated on 2024/04/24

写真a

 
KITO Seiji
 
Organization
Administrative Support Organizations Animal Research Support Center Professor
Title
Professor

Degree 3

  1. Doctor of Veterinary Science ( 1996.8   University of Wisconsin-Madison ) 

  2. Master of Science ( 1991.9   Kyoto University ) 

  3. Bachelor of Science ( 1989.3   Kyoto University ) 

Research Interests 3

  1. preimplantation embryo, gamete

  2. Fertilization

  3. Assisted reproductive technology

Research Areas 2

  1. Life Science / Laboratory animal science

  2. Life Science / Animal production science

Research History 6

  1. Nagoya University   Administrative Support Organizations Center for Animal Research and Education   Professor

    2017.4

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    Country:Japan

  2. Nagoya University   Administrative Support Organizations Animal Research Support Center   Manager of Higashiyama Laboratory Animal Facility

    2017.4

  3. The National Institutes for Quantum and Radiological Science and Technology   Department of Information Technology, Research Information Section   Chief Researcher

    2016.7 - 2017.3

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    Country:Japan

  4. MEXT   Research Promotion Bereau   Administrative Researcher

    2010.4 - 2011.3

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    Country:Japan

  5. National Institute of Radiological Sciences   Department of Engineering and Safety, Laboratory Animal and Genome Sciences Section   Researcher

    1998.4 - 2016.6

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    Country:Japan

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Education 3

  1. University of Wisconsin-Madison   Department of Animal Health and Biomedical Science   Reproductive Physiology

    1991.9 - 1996.8

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    Country: United States

  2. Kyoto University   Graduate School, Division of Agriculture   Department of Animal Science

    1989.4 - 1991.9

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    Country: Japan

  3. Kyoto University   Faculty of Agriculture   Department of Animal Science

    1984.4 - 1989.3

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    Country: Japan

Professional Memberships 6

  1. Japan Association for Laboratory Animal Science

    2018.4

  2. Japan Society for Ova Reseach

  3. Society for the Study of Reproduction

  4. The Society for Reproduction and Development

  5. The Japanese Association for Experimental Animal Technologists

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Committee Memberships 4

  1. 国立大学法人動物実験施設協議会   組織委員会委員  

    2018.4 - 2020.3   

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    Committee type:Academic society

  2. 国立大学法人動物実験施設協議会   遺伝子組換え動物委員会  

    2022.4   

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    Committee type:Academic society

  3. 国立大学法人動物実験施設協議会   幹事校  

    2022.4   

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    Committee type:Academic society

  4. 国立大学法人動物実験施設協議会   動物実験等適正化委員会  

    2022.4   

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    Committee type:Academic society

 

Papers 29

  1. Enhanced Effects of Chronic Restraint-Induced Psychological Stress on Total Body Fe-Irradiation-Induced Hematopoietic Toxicity in <i>Trp53</i>-Heterozygous Mice Reviewed International journal

    Wang, B; Katsube, T; Tanaka, K; Ninomiya, Y; Hirakawa, H; Liu, CH; Maruyama, K; Varès, G; Kito, S; Nakajima, T; Fujimori, A; Nenoi, M

    LIFE-BASEL   Vol. 12 ( 4 )   2022.4

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    Authorship:Lead author, Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/life12040565

    Web of Science

    PubMed

  2. Synergistic Effects of Chronic Restraint-Induced Stress and Low-Dose 56Fe-particle Irradiation on Induction of Chromosomal Aberrations in Trp53-Heterozygous Mice. Reviewed International journal

    Katsube T, Wang B, Tanaka K, Ninomiya Y, Hirakawa H, Liu C, Maruyama K, Vares G, Liu Q, Kito S, Nakajima T, Fujimori A, Nenoi M

    Radiation research     2021.4

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    Authorship:Lead author, Last author, Corresponding author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1667/RADE-20-00218.1

    PubMed

  3. Detection of Alzheimer's disease-related neuroinflammation by a PET ligand selective for glial versus vascular translocator protein. Reviewed International journal

    Ji B, Ono M, Yamasaki T, Fujinaga M, Zhang MR, Seki C, Aoki I, Kito S, Sawada M, Suhara T, Sahara N, Higuchi M

    Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism     page: 271678X21992457   2021.2

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    Authorship:Lead author, Last author, Corresponding author   Publishing type:Research paper (scientific journal)  

    DOI: 10.1177/0271678X21992457

    PubMed

  4. Development of Human Sodium/Iodide Symporter (hNIS) Transgenic Mouse as Resource of Stem Cells for in vivo Cell Tracking Reviewed International journal

    Inubushi M., Michikawa Y., Kito S., Takeuchi Y., Kitagawa Y., Saga T.

    EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING   Vol. 45   page: S624 - S625   2018.10

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    Authorship:Lead author, Last author, Corresponding author   Publishing type:Research paper (scientific journal)  

    Web of Science

  5. Regulation of Anxiety and Depression by Mitochondrial Translocator Protein-Mediated Steroidogenesis: the Role of Neurons Reviewed

    Barron AM, Higuchi M, Hattori S, Kito S, Suhara T, Ji B

    Molecular neurobiology   Vol. 58 ( 2 ) page: 550 - 563   2021.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s12035-020-02136-5

    Other Link: https://link.springer.com/article/10.1007/s12035-020-02136-5

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MISC 1

  1. Functional analysis of lysosomes during mouse preimplantation embryo development. Reviewed

    Tsukamoto S, Hara T, Yamamoto A, Ohta Y, Wada A, Ishida Y, Kito S, Nishikawa T, Minami N, Sato K, Kokubo T

    The Journal of reproduction and development   Vol. 59 ( 1 ) page: 33-9   2013

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    Language:English  

    PubMed

Presentations 2

  1. 哺乳類初期胚培養液開発の歩み Invited

    鬼頭 靖司

    日本実験動物技術者協会東海北陸支部第18回技術交流会  2023.2.11  日本実験動物技術者協会東海北陸支部

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    Event date: 2023.2

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:オンライン講演会   Country:Japan  

  2. 配偶子・初期胚培養液の開発 -黎明期、暗黒時代、ルネッサンス、そして現在の安定期まで- Invited

    鬼頭 靖司

    実験動物技術者協会 関東支部 REG部会 第20回特別講演会 

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    Event date: 2019.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:慶應義塾大学医学部   Country:Japan  

    本講演では、今は一般的に市販品として入手可能な初期胚培養用の培養液がどのような経緯を経て開発されてきたかについて主にげっ歯類を中心に紹介する。

KAKENHI (Grants-in-Aid for Scientific Research) 6

  1. Molecular Imaging of Transplanted Stem Cells Derived from Original NIS Transgenic Mouse

    Grant number:26293282  2014.4 - 2018.3

    INUBUSHI Masayuki

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    Authorship:Coinvestigator(s) 

    Using a transgenic mouse systemically and stabley expressing NIS genes (NIS-Tg mouse), we collaborated on the following 3 studies with specialists. (1) We succeeded in changing over its breeding from hetero-crossing to homo-crossing that increases possibility for higher gene expression, and almost established its inbred line (Kawasaki Med School). (2) We succeeded in in-vivo quantitative analysis of the function of stem-cell sheets made with stem cells derived from the NIS-Tg mouse, which contributed to the development of a novel iPS-sheet therapy for myocardial infarction in humans (NCVC). (3) We performed transplantation of stem cells derived from the NIS-Tg mouse to irradiated mice, and investigated how and with which specific character of stem cells the mice could be survived after high-dose radiation exposure (NIRS).

  2. Development of hereditary non-polyposis colon cancer model using the Mlh1-deficient heterozygous mice

    Grant number:22501002  2010 - 2012

    KAKINUMA Shizuko

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid) 

    Heterozygous germline mutations of mismatch repair (MMR) genes cause human hereditary non-polyposis colon cancer, and homozygous mutations are manifested by early onset of childhood T- or B-cell leukemia. In order to develop models for both colon cancer and lymphoma, we examined induction protocols with radiation and an inflammatory agent in Mlh1-/- homozygous and Mlh1+/- heterozygous mice. The protocols we established reproducibly induced colon cancer in Mlh1-/- homozygous and Mlh1+/- heterozygous mice, and lymphoma in Mlh1-/- homozygous mice using radiation and/or an inflammatory agent.

  3. 精子形成期の半数体特異的遺伝子発現を利用した単性動物の作出

    Grant number:21658094  2009 - 2010

    南 直治郎

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    本研究においては、雌雄の産み分けが可能な雄を作出する目的で、22年度は下記の2点について検討を行った。
    (1) 導入遺伝子産物であるmRNAおよび翻訳後のタンパク質が精子形成期の細胞間架橋を自由に通過できないような仕組みを開発する。
    (2) 遺伝子導入された染色体を持つ精子が受精できない仕組みを開発する。
    (1)についてはSmok1遺伝子の5'および3'非翻訳領域が細胞間架橋に関与することが明らかになったが、その正確な遺伝子配列は公表されていない。そこで、それぞれの配列のクローニングを行い、実際にゲノム上およびcDNA中にその配列が存在することを確認し、遺伝子組換えマウス作製のための組換えベクターを作出した。(2)については精子アクロソーム膜上に発現し、受精に関与することが示唆されているタンパク質をコードする遺伝子にRSK3遺伝子の3'UTRを接続した融合遺伝子を構築し、この遺伝子をプロタミン制御下で発現する遺伝子組換えマウスを作製した。作出したマウスのF1個体を使って、受精に及ぼす影響および細胞間架橋の通過について検討した。いくつかの系統では正常に産子が得られたため、受精には影響していないと考えられたが、不妊傾向が認められる系統も得られており、現在解析を行っている。

  4. Analysis of oocyte-specific gene, Oog1 and its involvement in molecular basis of zygotic gene activation

    Grant number:19380158  2007 - 2009

    MINAMI Naojiro

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    In the present study, we investigated the function of oocyte-specific gene, Oog1. The gene translocates to the nucleus of fertilized embryos at the time of zygotic gene activation. In order to analyze the function of the gene, we produced the transgenic mice in which the mRNA of Oog1 is knockdown. In addition, we investigated the protein expression of Oog1 during female meiosis and it is revealed that the protein exists in the oocyte nucleus at the prophase of meiosis. In another experiments, we identified the regulatory regions of Oog1 which controls the expression of the gene.

  5. Development of the gene deletion model rats by random mutagenesis.

    Grant number:16500282  2004 - 2005

    HARADA Yoshinobu

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    Random mutagenesis with chlorambucil was performed in order to produce some new mutant rats. To identify the optimal quantity of chlorambucil and the optimal mating time for mutagenesis, we counted the number of new born rats born from female rats mated with the male rat medicated with several dose of chlorambucil. Consequently, it became clear that between 2.5mg and 15mg per kg weight of chlorambucil are good for medication, and it is good to perform mating between three weeks and six weeks after the medication of chlorambucil. We obtained several dwarfish rats from BN and F344 strans. Dwarfish rats whose sizes of the body were about 30% of normal rats were also observed. We also obtained a mutant rat with fiblosarcoma from the BN strain. This fiblosarcoma has grown up to 5cm or more in the diameter within 2 weeks. Unfortunately, we have not determined the genome region of delition of this rat by quontitative PCR analysis. Future experiments will focus on searching this genome region of delition by microarray or BAC array analyses.

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