Organization
Administrative Support Organizations Animal Research Support Center Professor
Title
Professor
Homepage
KITO Seiji
Degree 3
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Doctor of Veterinary Science （ 1996.8 University of Wisconsin-Madison ）
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Master of Science （ 1991.9 Kyoto University ）
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Bachelor of Science （ 1989.3 Kyoto University ）
Research Interests 3
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preimplantation embryo, gamete
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Fertilization
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Assisted reproductive technology
Research Areas 2
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Life Science / Laboratory animal science
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Life Science / Animal production science
Research History 6
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Nagoya University Administrative Support Organizations Center for Animal Research and Education Professor
2017.4
Country：Japan
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Nagoya University Administrative Support Organizations Animal Research Support Center Manager of Higashiyama Laboratory Animal Facility
2017.4
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The National Institutes for Quantum and Radiological Science and Technology Department of Information Technology, Research Information Section Chief Researcher
2016.7 - 2017.3
Country：Japan
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MEXT Research Promotion Bereau Administrative Researcher
2010.4 - 2011.3
Country：Japan
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National Institute of Radiological Sciences Department of Engineering and Safety, Laboratory Animal and Genome Sciences Section Researcher
1998.4 - 2016.6
Country：Japan
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Cornell University Department of Physiology Post-Doctoral Research Associate
1996.9 - 1998.2
Country：United States
Education 3
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University of Wisconsin-Madison Department of Animal Health and Biomedical Science Reproductive Physiology
1991.9 - 1996.8
Country： United States
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Kyoto University Graduate School, Division of Agriculture Department of Animal Science
1989.4 - 1991.9
Country： Japan
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Kyoto University Faculty of Agriculture Department of Animal Science
1984.4 - 1989.3
Country： Japan
Professional Memberships 6
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Japan Association for Laboratory Animal Science
2018.4
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Japan Society for Ova Reseach
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Society for the Study of Reproduction
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The Society for Reproduction and Development
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The Japanese Association for Experimental Animal Technologists
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The Japanese Association for Experimental Animal Technologists
Committee Memberships 4
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国立大学法人動物実験施設協議会 組織委員会委員
2018.4 - 2020.3
Committee type：Academic society
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国立大学法人動物実験施設協議会 遺伝子組換え動物委員会
2022.4
Committee type：Academic society
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国立大学法人動物実験施設協議会 動物実験等適正化委員会
2022.4
Committee type：Academic society
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国立大学法人動物実験施設協議会 幹事校
2022.4
Committee type：Academic society
Papers 29
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Enhanced Effects of Chronic Restraint-Induced Psychological Stress on Total Body Fe-Irradiation-Induced Hematopoietic Toxicity in <i>Trp53</i>-Heterozygous Mice Reviewed International journal
Wang, B; Katsube, T; Tanaka, K; Ninomiya, Y; Hirakawa, H; Liu, CH; Maruyama, K; Varès, G; Kito, S; Nakajima, T; Fujimori, A; Nenoi, M
LIFE-BASEL Vol. 12 ( 4 ) 2022.4
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Synergistic Effects of Chronic Restraint-Induced Stress and Low-Dose 56Fe-particle Irradiation on Induction of Chromosomal Aberrations in Trp53-Heterozygous Mice. Reviewed International journal
Katsube T, Wang B, Tanaka K, Ninomiya Y, Hirakawa H, Liu C, Maruyama K, Vares G, Liu Q, Kito S, Nakajima T, Fujimori A, Nenoi M
Radiation research 2021.4
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Detection of Alzheimer's disease-related neuroinflammation by a PET ligand selective for glial versus vascular translocator protein. Reviewed International journal
Ji B, Ono M, Yamasaki T, Fujinaga M, Zhang MR, Seki C, Aoki I, Kito S, Sawada M, Suhara T, Sahara N, Higuchi M
Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism page： 271678X21992457 2021.2
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Development of Human Sodium/Iodide Symporter (hNIS) Transgenic Mouse as Resource of Stem Cells for in vivo Cell Tracking Reviewed International journal
Inubushi M., Michikawa Y., Kito S., Takeuchi Y., Kitagawa Y., Saga T.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING Vol. 45 page： S624 - S625 2018.10
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Barron AM, Higuchi M, Hattori S, Kito S, Suhara T, Ji B
Molecular neurobiology Vol. 58 ( 2 ) page： 550 - 563 2021.2
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Digenic mutations in ALDH2 and ADH5 impair formaldehyde clearance and cause a multisystem disorder, AMeD syndrome Reviewed
Oka Yasuyoshi, Hamada Motoharu, Nakazawa Yuka, Muramatsu Hideki, Okuno Yusuke, Higasa Koichiro, Shimada Mayuko, Takeshima Honoka, Hanada Katsuhiro, Hirano Taichi, Kawakita Toshiro, Sakaguchi Hirotoshi, Ichimura Takuya, Ozono Shuichi, Yuge Kotaro, Watanabe Yoriko, Kotani Yuko, Yamane Mutsumi, Kasugai Yumiko, Tanaka Miyako, Suganami Takayoshi, Nakada Shinichiro, Mitsutake Norisato, Hara Yuichiro, Kato Kohji, Mizuno Seiji, Miyake Noriko, Kawai Yosuke, Tokunaga Katsushi, Nagasaki Masao, Kito Seiji, Isoyama Keiichi, Onodera Masafumi, Kaneko Hideo, Matsumoto Naomichi, Matsuda Fumihiko, Matsuo Keitaro, Takahashi Yoshiyuki, Mashimo Tomoji, Kojima Seiji, Ogi Tomoo
SCIENCE ADVANCES Vol. 6 ( 51 ) 2020.12
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Effects of high calcium levels on the disturbed extrusion of the second polar body duringin vitrofertilization in C3H/He mouse substrains Reviewed
Ohta-Takada Y., Nagao Y., Kito S.
ZYGOTE Vol. 28 ( 1 ) page： 83 - 85 2020.2
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Effects of high calcium levels on the disturbed extrusion of the second polar body during in vitro fertilization in C3H/He mouse substrains Reviewed
Y. Ohta-Takada, Y. Nagao and S. Kito
Zygote 2019.10
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Barron AM, Ji B, Kito S, Suhara T, Higuchi M.
Biochemical Journal Vol. 475 ( 1 ) page： 75-85 2018.1
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Multimodal Imaging for DREADD-Expressing Neurons in Living Brain and Their Application to Implantation of iPSC-Derived Neural Progenitors. Reviewed
Ji B, Kaneko H, Minamimoto T, Inoue H, Takeuchi H, Kumata K, Zhang MR, Aoki I, Seki C, Ono M, Tokunaga M, Tsukamoto S, Tanabe K, Shin RM, Minamihisamatsu T, Kito S, Richmond BJ, Suhara T, Higuchi M
The Journal of neuroscience : the official journal of the Society for Neuroscience Vol. 36 ( 45 ) page： 11544-11558 2016.11
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Ohta Y, Nagao Y, Minami N, Tsukamoto S, Kito S.
Zygote. Vol. 24 ( 4 ) page： 603-616 2016.8
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Ionizing radiation, inflammation, and their interactions in colon carcinogenesis in Mlh1-deficient mice. Reviewed
Morioka T, Miyoshi-Imamura T, Blyth BJ, Kaminishi M, Kokubo T, Nishimura M, Kito S, Tokairin Y, Tani S, Murakami-Murofushi K, Yoshimi N, Shimada Y, Kakinuma S
Cancer science Vol. 106 ( 3 ) page： 217-26 2015.3
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Fluorescence-based visualization of autophagic activity predicts mouse embryo viability. Reviewed
Tsukamoto S, Hara T, Yamamoto A, Kito S, Minami N, Kubota T, Sato K, Kokubo T
Scientific reports Vol. 4 page： 4533 2014.3
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Disruption of Aspm causes microcephaly with abnormal neuronal differentiation Reviewed
Fujimori A, Itoh K, Goto S, Hirakawa H, Wang B, Kokubo T, Kito S, Tsukamoto S, Fushiki S.
Brain and Development Vol. 36 ( 8 ) page： 661-669 2013.11
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The promoter of the oocyte-specific gene, Oog1, functions in both male and female meiotic germ cells in transgenic mice. Reviewed
Ishida M, Okazaki E, Tsukamoto S, Kimura K, Aizawa A, Kito S, Imai H, Minami N
PloS one Vol. 8 ( 7 ) page： e68686 2013
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High osmolality vitrification: a new method for the simple and temperature-permissive cryopreservation of mouse embryos. Reviewed
Mochida K, Hasegawa A, Li MW, Fray MD, Kito S, Vallelunga JM, Lloyd KC, Yoshiki A, Obata Y, Ogura A
PloS one Vol. 8 ( 1 ) page： e49316 2013
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Genomic and gene expression signatures of radiation in medulloblastomas after low-dose irradiation in Ptch1 heterozygous mice. Reviewed
Ishida Y, Takabatake T, Kakinuma S, Doi K, Yamauchi K, Kaminishi M, Kito S, Ohta Y, Amasaki Y, Moritake H, Kokubo T, Nishimura M, Nishikawa T, Hino O, Shimada Y
Carcinogenesis Vol. 31 ( 9 ) page： 1694-701 2010.9
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Superovulatory response, oocyte spontaneous activation, and embryo development in WMN/Nrs inbred rats. Reviewed
Kito S, Yano H, Ohta Y, Tsukamoto S
Experimental animals Vol. 59 ( 1 ) page： 35-45 2010
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In vitro fertilization in inbred BALB/c mice II: effects of lactate, osmolarity and calcium on in vitro capacitation. Reviewed
Kito S, Ohta Y
Zygote (Cambridge, England) Vol. 16 ( 3 ) page： 259-70 2008.8
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In vitro fertilization in inbred BALB/c mice I: isotonic osmolarity and increased calcium-enhanced sperm penetration through the zona pellucida and male pronuclear formation. Reviewed
Kito S, Ohta Y
Zygote (Cambridge, England) Vol. 16 ( 3 ) page： 249-57 2008.8
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Differential effects of low- and high-dose X-rays on N-ethyl-N-nitrosourea-induced mutagenesis in thymocytes of B6C3F1 gpt-delta mice. Reviewed
Yamauchi K, Kakinuma S, Sudo S, Kito S, Ohta Y, Nohmi T, Masumura K, Nishimura M, Shimada Y
Mutation research Vol. 640 ( 1-2 ) page： 27-37 2008.4
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Developmental responses of 2-cell embryos to oxygen tension and bovine serum albumin in Wistar rats. Reviewed
Kito S, Kaneko Y, Yano H, Tateno S, Ohta Y
Experimental animals Vol. 57 ( 2 ) page： 123-8 2008.4
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Medium effects on capacitation and sperm penetration through the zona pellucida in inbred BALB/c spermatozoa. Reviewed
Kito S, Ohta Y
Zygote (Cambridge, England) Vol. 13 ( 2 ) page： 145-53 2005.5
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Severe growth retardation and short life span of double-mutant mice lacking Xpa and exon 15 of Xpg. Reviewed
Shiomi N, Mori M, Kito S, Harada YN, Tanaka K, Shiomi T
DNA repair Vol. 4 ( 3 ) page： 351-7 2005.3
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Improved in vitro fertilization and development by use of modified human tubal fluid and applicability of pronucleate embryos for cryopreservation by rapid freezing in inbred mice. Reviewed
Kito S, Hayao T, Noguchi-Kawasaki Y, Ohta Y, Hideki U, Tateno S
Comparative medicine Vol. 54 ( 5 ) page： 564-70 2004.10
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Identification of the XPG region that causes the onset of Cockayne syndrome by using Xpg mutant mice generated by the cDNA-mediated knock-in method. Reviewed
Shiomi N, Kito S, Oyama M, Matsunaga T, Harada YN, Ikawa M, Okabe M, Shiomi T.
Molecular and cellular Biology Vol. 24 ( 9 ) page： 3712-9 2004.5
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Growth retardation and skin abnormalities of the Recql4-deficient mouse. Reviewed
Hoki Y, Araki R, Fujimori A, Ohhata T, Koseki H, Fukumura R, Nakamura M, Takahashi H, Noda Y, Kito S, Abe M
Human molecular genetics Vol. 12 ( 18 ) page： 2293-9 2003.9
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Evaluation of developmental competence of vitrified-warmed early cleavage stage embryos and their application for chimeric mouse production. Reviewed
Kito S, Noguchi Y, Ohta Y, Ohhata T, Abe M, Shiomi N, Shiomi T.
Experimental Animals Vol. 52 ( 2 ) page： 179-83 2003.3
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Developmental responses of two substrains of in vitro fertilized C57BL/6J mouse embryos to oxygen and amino acids. Reviewed
Kito S, Noguchi Y, Ohta Y
Experimental animals Vol. 52 ( 1 ) page： 63-6 2003.1
MISC 1
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Functional analysis of lysosomes during mouse preimplantation embryo development. Reviewed
Tsukamoto S, Hara T, Yamamoto A, Ohta Y, Wada A, Ishida Y, Kito S, Nishikawa T, Minami N, Sato K, Kokubo T
The Journal of reproduction and development Vol. 59 ( 1 ) page： 33-9 2013
Presentations 2
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哺乳類初期胚培養液開発の歩み Invited
鬼頭 靖司
日本実験動物技術者協会東海北陸支部第18回技術交流会 2023.2.11 日本実験動物技術者協会東海北陸支部
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配偶子・初期胚培養液の開発 －黎明期、暗黒時代、ルネッサンス、そして現在の安定期まで－ Invited
鬼頭 靖司
実験動物技術者協会 関東支部 REG部会 第20回特別講演会
KAKENHI (Grants-in-Aid for Scientific Research) 6
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Molecular Imaging of Transplanted Stem Cells Derived from Original NIS Transgenic Mouse
Grant number：26293282 2014.4 - 2018.3
INUBUSHI Masayuki
Authorship：Coinvestigator(s)
Using a transgenic mouse systemically and stabley expressing NIS genes (NIS-Tg mouse), we collaborated on the following 3 studies with specialists. (1) We succeeded in changing over its breeding from hetero-crossing to homo-crossing that increases possibility for higher gene expression, and almost established its inbred line (Kawasaki Med School). (2) We succeeded in in-vivo quantitative analysis of the function of stem-cell sheets made with stem cells derived from the NIS-Tg mouse, which contributed to the development of a novel iPS-sheet therapy for myocardial infarction in humans (NCVC). (3) We performed transplantation of stem cells derived from the NIS-Tg mouse to irradiated mice, and investigated how and with which specific character of stem cells the mice could be survived after high-dose radiation exposure (NIRS).
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Development of hereditary non-polyposis colon cancer model using the Mlh1-deficient heterozygous mice
Grant number：22501002 2010 - 2012
KAKINUMA Shizuko
Authorship：Collaborating Investigator(s) (not designated on Grant-in-Aid)
Heterozygous germline mutations of mismatch repair (MMR) genes cause human hereditary non-polyposis colon cancer, and homozygous mutations are manifested by early onset of childhood T- or B-cell leukemia. In order to develop models for both colon cancer and lymphoma, we examined induction protocols with radiation and an inflammatory agent in Mlh1-/- homozygous and Mlh1+/- heterozygous mice. The protocols we established reproducibly induced colon cancer in Mlh1-/- homozygous and Mlh1+/- heterozygous mice, and lymphoma in Mlh1-/- homozygous mice using radiation and/or an inflammatory agent.
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精子形成期の半数体特異的遺伝子発現を利用した単性動物の作出
Grant number：21658094 2009 - 2010
南 直治郎
Authorship：Coinvestigator(s)
本研究においては、雌雄の産み分けが可能な雄を作出する目的で、22年度は下記の2点について検討を行った。
(1) 導入遺伝子産物であるmRNAおよび翻訳後のタンパク質が精子形成期の細胞間架橋を自由に通過できないような仕組みを開発する。
(2) 遺伝子導入された染色体を持つ精子が受精できない仕組みを開発する。
(1)についてはSmok1遺伝子の5'および3'非翻訳領域が細胞間架橋に関与することが明らかになったが、その正確な遺伝子配列は公表されていない。そこで、それぞれの配列のクローニングを行い、実際にゲノム上およびcDNA中にその配列が存在することを確認し、遺伝子組換えマウス作製のための組換えベクターを作出した。(2)については精子アクロソーム膜上に発現し、受精に関与することが示唆されているタンパク質をコードする遺伝子にRSK3遺伝子の3'UTRを接続した融合遺伝子を構築し、この遺伝子をプロタミン制御下で発現する遺伝子組換えマウスを作製した。作出したマウスのF1個体を使って、受精に及ぼす影響および細胞間架橋の通過について検討した。いくつかの系統では正常に産子が得られたため、受精には影響していないと考えられたが、不妊傾向が認められる系統も得られており、現在解析を行っている。 -
Analysis of oocyte-specific gene, Oog1 and its involvement in molecular basis of zygotic gene activation
Grant number：19380158 2007 - 2009
MINAMI Naojiro
Authorship：Coinvestigator(s)
In the present study, we investigated the function of oocyte-specific gene, Oog1. The gene translocates to the nucleus of fertilized embryos at the time of zygotic gene activation. In order to analyze the function of the gene, we produced the transgenic mice in which the mRNA of Oog1 is knockdown. In addition, we investigated the protein expression of Oog1 during female meiosis and it is revealed that the protein exists in the oocyte nucleus at the prophase of meiosis. In another experiments, we identified the regulatory regions of Oog1 which controls the expression of the gene.
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Development of the gene deletion model rats by random mutagenesis.
Grant number：16500282 2004 - 2005
HARADA Yoshinobu
Authorship：Coinvestigator(s)
Random mutagenesis with chlorambucil was performed in order to produce some new mutant rats. To identify the optimal quantity of chlorambucil and the optimal mating time for mutagenesis, we counted the number of new born rats born from female rats mated with the male rat medicated with several dose of chlorambucil. Consequently, it became clear that between 2.5mg and 15mg per kg weight of chlorambucil are good for medication, and it is good to perform mating between three weeks and six weeks after the medication of chlorambucil. We obtained several dwarfish rats from BN and F344 strans. Dwarfish rats whose sizes of the body were about 30% of normal rats were also observed. We also obtained a mutant rat with fiblosarcoma from the BN strain. This fiblosarcoma has grown up to 5cm or more in the diameter within 2 weeks. Unfortunately, we have not determined the genome region of delition of this rat by quontitative PCR analysis. Future experiments will focus on searching this genome region of delition by microarray or BAC array analyses.
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Development of the gene deletion model rats by random mutagenesis
Grant number：14580805 2002 - 2003
HARADA Yoshinobu
Authorship：Coinvestigator(s)
Random mutagenesis with chlorambucil was performed in order to produce some new mutant rats. To identify the optimal quantity of chlorambucil and the optimal mating time for mutagenesis, we counted the number of new born rats born from female rats mated with the male rat medicated with several dose of chlorambucil. Consequently, it became clear that between 5mg and 15mg per kg weight of chlorambucil are good for medication, and it is good to perform mating between three weeks and six weeks after the medication of chlorambucil. The female (G1 generation) rat was obtained as a result of crossing between the male rat of BN strain medicated with 10mg chlorambucil and a normal F344 female rat. This female rat has the capability to conceive. A male rat (G3 generation) was obtained from a cross the female rat (G2 generation) with the male rat (G3 generation). Then we crossed these rats and obtained six rats. When these six rats were bred for about one year, one of the rats had skin cancer and another one showed diabetes symptom. It was presumed that these rats are the mutants by which mutation was occurred in some genes. In addition, the dwarfish rat whose size of the body is about 30% of a normal rat was also observed. In almost all cases, the dwarfish rats died before weaning, but a few dwarfish rats grew to the adult. Although the adult dwarfish rats was able to be conceived, they born some dwarfish rats by mating with a normal rat similarly. In order to confirm to which gene mutation has happened, gene expression analysis was performed for the G5 generation rats using DNA microarray. Consequently, it was proved that at least ten kinds of genes was not expressed in the G5 rats. These genes were presumed that the expression of these genes was suppressed by deletion type of mutation in each genes.