Organization
Institute for Glyco-core Research Lecturer
Graduate School
Graduate School of Medicine
Graduate School of Medicine
Graduate School of Medicine
Title
Lecturer
TASHIMA Yuko
Degree 2
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博士（医学） （ 2006.3 大阪大学 ）
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修士（薬学） （ 2002.3 武庫川女子大学 ）
Research Interests 2
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Glycobiology
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Molecular biology
Research Areas 2
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Life Science / Molecular biology
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Life Science / Cell biology / Glycobiology
Current Research Project and SDGs 1
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糖鎖修飾によるタンパク質の機能制御や発現制御の機構の解明
Research History 6
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Nagoya University Lecturer
2022.5
Country：Japan
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Nagoya University Assistant Professor
2016.10 - 2022.4
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大阪大学微生物病研究所 免疫不全疾患研究分野 助教
2014.4 - 2016.9
Country：Japan
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国立循環器病研究センター研究所 分子病態部 流動研究員
2012.4 - 2014.3
Country：Japan
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Albert Einstein College of Medicine of Yeshiva University Dep. Cell Biology
2007.4 - 2012.2
Country：United States
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大阪大学微生物病研究所 免疫不全疾患研究分野 特任研究員
2006.4 - 2007.3
Country：Japan
Education 2
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Osaka University Graduate School, Division of Medical Sciences
- 2006.3
Country： Japan
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Mukogawa Women's University Faculty of Pharmaceutical Science
- 1996.4
Country： Japan
Professional Memberships 4
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Society for Glycobiology
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日本血栓止血学会
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The molecular biology society of Japan
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The Japanese Society of Carbohydrate Research
Committee Memberships 1
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日本生化学会中部支部 幹事(第3)
2020.9 - 2022.8
Committee type：Academic society
Awards 1
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第40回日本血栓止血学会学術集会、若手奨励賞
2018.6 日本血栓止血学会
田嶌優子
Papers 24
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Characterization of galactosyltransferase and sialyltransferase genes mediating the elongation of the extracellular O-GlcNAc glycans.
Tsukamoto Y, Tsukamoto N, Saiki W, Tashima Y, Furukawa JI, Kizuka Y, Narimatsu Y, Clausen H, Takeuchi H, Okajima T
Biochemical and biophysical research communications Vol. 703 page： 149610 2024.4
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LYSET is a New Factor Critical for Lysosomal Enzyme Transport and Viral Infection
Tashima, Y
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY Vol. 35 ( 207 ) page： E86 - E86 2023.9
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Secretory expression of mammalian NOTCH tandem epidermal growth factor-like repeats based on increased O-glycosylation
Zhang Ailing, Tsukamoto Yohei, Takeuchi Hideyuki, Nishiwaki Kimitoshi, Tashima Yuko, Okajima Tetsuya
ANALYTICAL BIOCHEMISTRY Vol. 656 page： 114881 2022.11
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Proteostatic Function of N-glycan on Procollagen
Tashima Yuko
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY Vol. 34 ( 200 ) page： E81 - E82 2022.7
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Glycoproteomics of NOTCH1 EGF repeat fragments overexpressed with different glycosyltransferases in HEK293T cells reveals insights into O-GlcNAcylation of NOTCH1.
Tsukamoto Y, Ogawa M, Yogi K, Tashima Y, Takeuchi H, Okajima T
Glycobiology 2022.3
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Contribution of Glucosylceramide Synthase to the Proliferation of Mouse Osteoblasts.
Mishima Y, Hamamura K, Kato H, Furukawa K, Tashima Y, Okajima T, Kondo H, Sato T, Miyazawa K, Goto S, Togari A
In vivo (Athens, Greece) Vol. 35 ( 6 ) page： 3111 - 3123 2021.11
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Differential use of p24 family members as cargo receptors for the transport of glycosylphosphatidylinositol-anchored proteins and Wnt1.
Tashima Y, Hirata T, Maeda Y, Murakami Y, Kinoshita T
Journal of biochemistry 2021.10
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Bioinformatics and Functional Analyses Implicate Potential Roles for EOGT and L-fringe in Pancreatic Cancers
Barua Rashu, Mizuno Kazuyuki, Tashima Yuko, Ogawa Mitsutaka, Takeuchi Hideyuki, Taguchi Ayumu, Okajima Tetsuya
MOLECULES Vol. 26 ( 4 ) 2021.2
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Enzyme assay of Fringe.
Nishihara S, Angata K, Aoki-Kinoshita KF, Hirabayashi J, Tashima Y
2021
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細胞外O-GlcNAcの構造と機能 Invited Reviewed
田嶌 優子、岡島徹也
生化学 みにれびゅう Vol. 92 ( 6 ) page： 833 - 837 2020.12
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N-glycans on EGF domain-specific O-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization
Alam Sayad Md Didarul, Tsukamoto Yohei, Ogawa Mitsutaka, Senoo Yuya, Ikeda Kazutaka, Tashima Yuko, Takeuchi Hideyuki, Okajima Tetsuya
GLYCOBIOLOGY Vol. 30 ( 12 ) page： 1031 - 1032 2020.12
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Glycoproteomic analysis identifies cryptdin-related sequence 1 as O-glycosylated protein modified with alpha 1,2-fucose in the small intestine
Hashiguchi Hiroki, Tsukamoto Yohei, Ogawa Mitsutaka, Tashima Yuko, Takeuchi Hideyuki, Nakamura Masanao, Kawashima Hiroki, Fujishiro Mitsuhiro, Okajima Tetsuya
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS Vol. 695 page： 108653 2020.11
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N-Glycans on EGF domain-specificO-GlcNAc transferase (EOGT) facilitate EOGT maturation and peripheral endoplasmic reticulum localization
Alam Sayad Md. Didarul, Tsukamoto Yohei, Ogawa Mitsutaka, Senoo Yuya, Ikeda Kazutaka, Tashima Yuko, Takeuchi Hideyuki, Okajima Tetsuya
JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 295 ( 25 ) page： 8560 - 8574 2020.6
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Contribution of extracellular O-GlcNAc to the stability of folded epidermal growth factor-like domains and Notch1 trafficking
Ogawa Mitsutaka, Tashima Yuko, Sakaguchi Yamato, Takeuchi Hideyuki, Okajima Tetsuya
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Vol. 526 ( 1 ) page： 184 - 190 2020.5
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Congenital diseases caused by defective O-glycosylation of Notch receptors
Tashima Yuko, Okajima Tetsuya
NAGOYA JOURNAL OF MEDICAL SCIENCE Vol. 80 ( 3 ) page： 299-307 2018.8
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Plasminogen Tochigi mice exhibit phenotypes similar to wild-type mice under experimental thrombotic conditions
Tashima Yuko, Banno Fumiaki, Kita Toshiyuki, Matsuda Yasuyuki, Yanamoto Hiroji, Miyata Toshiyuki
PLOS ONE Vol. 12 ( 7 ) page： e0180981 2017.7
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Influence of ADAMTS13 deficiency on venous thrombosis in mice Invited Reviewed
Y.Tashima, F.Banno, M.Akiyama, T.Miyata
Thrombosis and Haemostasis Vol. 114 ( 1 ) page： 206 - 207 2015
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Antibodies that Detect O-GlcNAc on the Extracellular Domain of Cell Surface Glycoproteins Reviewed
Y.Tashima, P.Stanley
The Journal of Biological Chemistry Vol. 289 ( 16 ) page： 11132 - 11142 2014
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Galactose differentially modulates lunatic and manic Fringe effects on Delta1-induced NOTCH signaling Reviewed
X.Hou, *Y.Tashima, P.Stanley
The Journal of Biological Chemistry Vol. 287 ( 1 ) page： 474 - 483 2012
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Roles of Pofut1 and O-fucose in mammalian Notch signaling Reviewed
The Journal of Biological Chemistry Vol. 283 ( 20 ) page： 13638 - 13651 2008
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Fatty acid remodeling of GPI-anchored proteins is required for their raft association Reviewed
Y.Maeda, Y.Tashima, T.Houjou, M.Fujita, T.Yoko-O, Y.Jigami, R.Taguchi, T.Kinoshita
Molecular Biology of the Cell Vol. 18 ( 4 ) page： 1497 - 1506 2007
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PGAP2 is essential for correct processing and stable expression of GPI-anchored proteins Reviewed
Y.Tashima, R.Taguchi, C.Murata, H.Ashida, T.Kinoshita, Y.Maeda
Molecular Biology of the Cell Vol. 17 ( 3 ) page： 1410 - 1420 2006
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The initial enzyme for glycosylphosphatidylinositol biosynthesis requires PIG-Y, a seventh component Reviewed
Y.Murakami, U.Siripanyaphinyo, Y.Hong, Y.Tashima, Y.Maeda, T.Kinoshita
Molecular Biology of the Cell Vol. 16 ( 11 ) page： 5236 - 5246 2005
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Inositol-deacylation of glycosylphosphatidylinositol-anchored proteins is mediated by mammalian PGAP1 and yeast Bst1p Reviewed
S.Tanaka, Y.Maeda, Y.Tashima, T.Kinoshita
The Journal of Biological Chemistry Vol. 279 ( 14 ) page： 14256 - 14263 2004
Books 3
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新・止血血栓血管学 凝固と炎症、 ２部 ショック・敗血症と凝血・炎症系
木下タロウ、田嶌優子（ Role： Sole author , １８凝固系と補体系反応：PNHを含む）
金芳堂 2015 （ ISBN:978-4-7653-1646-0 ）
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プリンシプル血液疾患の臨床、よくわかる血栓•止血異常の診療
宮田敏行、田嶌優子（ Role： Sole author , 第１章 血栓止血異常症を理解するために、B 凝固反応を理解する）
中山書店 2014.11 （ ISBN:978-4-521-73780-5 ）
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実験医学別冊 タンパク質の翻訳後修飾解析プロトコール
田嶌優子、前田祐輔（ Role： Joint author , ６章 脂質修飾の解析、５. GPIアンカー型タンパク質の解析）
羊土社 2005.12 （ ISBN:978-4-89706-418-5 ）
Presentations 7
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Roles of O-glycans on NOTCH receptors for folding control in the endoplasmic reticulum
Yuko Tashima
2023.9.7
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Roles of O-fucose and O-glucose for the Effective Transport of DLK1 to the Cell Surface Invited International conference
Yuko Tashima
Glycobiology Gordon Research Conference, Biological Roles of Glycans as Major Building Blocks of Life 0223.3.13 Glycobiology Gordon Research Conference
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Roles of O-fucose and O-glucose for the Effective Transport of DLK1 to the Cell Surface Invited International conference
Yuko Tashima
Glycobiology Gordon Research Conference, Biological Roles of Glycans as Major Building Blocks of Life 0223.3.13 Glycobiology Gordon Research Conference
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O-fucose and O-glucose modifications are important for the effective transport of DLK1 to the cell surface
2022.11.9
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O-fucose and O-glucose modifications are important for the effective transport of DLK1 to the cell surface
2022.11.9
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The deficiency of O-fucose or O-glucose delays the transport of DLK1
2022.9.29
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Roles of POFUT1 and POGLUT1 for the effective transport of DLK1 to the cell surface International conference
Yuko Tashima, Wakako Goto, Yohei Tsukamoto, Mitsutaka Ogawa, Hideyuki Takeuchi, Tetsuya Okajima
2022 Annual meeting 2022.11.14 Society for Glycobiology
Research Project for Joint Research, Competitive Funding, etc. 1
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脳小血管病CADASILのNOTCH3変異体の糖鎖修飾構造の解明
2022年度(第30回)研究助成
Authorship：Principal investigator Grant type：Competitive
Grant amount：\500000
KAKENHI (Grants-in-Aid for Scientific Research) 4
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構造異常NOTCH3がO型糖鎖を介した調節で適切に除去される仕組みの解明
Grant number：24K10075 2024.4 - 2027.3
科学研究費助成事業 基盤研究(C)
田嶌 優子, 大橋 憲太郎
Authorship：Principal investigator
Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）
脳小血管病CADASILの患者では、NOTCH3遺伝子に変異があり、血管平滑筋の近傍にNOTCH3の凝集体を生じて脳小血管の変性に至る。NOTCH3遺伝子の変異と分子病態との関係は不明で、現在も原因療法がない。申請者は、異常NOTCH3の凝集と分解にO型糖鎖修飾が重要であるという知見を得た。本研究では、構造異常NOTCH3がO型糖鎖を介した調節機構によって凝集し分解する分子機構を解明する。本研究成果で、O型糖鎖修飾を変化させて構造異常NOTCH3を分解に誘導して、CADASIL疾患を根本治療するための基盤を構築する。
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Significance of structural diversity of O-glucose glycans in Notch signaling
Grant number：19KK0195 2019.10 - 2022.3
Grants-in-Aid for Scientific Research Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))
Authorship：Coinvestigator(s)
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Grant number：16K14696 2016.4 - 2019.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research
TASHIMA YUKO
Authorship：Principal investigator
Grant amount：\3770000 （ Direct Cost: \2900000 、 Indirect Cost：\870000 ）
Glycosylphosphatidylinositol (GPI) tether a hydrophilic protein on the plasma membrane. Synthesis of GPI-anchored proteins (GPI-APs) in the ER and its lipid remodeling in the Golgi have been studied very well, but the transport process to the cell surface from the Golgi have not been known well. We newly established a method to monitor the transport of GPI-APs to the cell surface from the Golgi using retention using selective hooks system [Boncompain G et al., Nature methods, 2012] and HEK293 cells. To identify factor proteins for transport of GPI-APs to the cell surface from the Golgi, we used this method by introducing reporter cells with CRISPR/Cas9 and single guide RNA library. We sorted mutant cells that delayed transport of a reporter GPI to the cell surface from the Golgi. The candidate factor proteins to be related with transport of GPI-APs are analyzing by next generation sequencing.
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遺伝子改変マウスを用いたプラスミノーゲン栃木変異と血栓症の検討
Grant number：25860801 2013.4 - 2015.3
科学研究費補助金 若手研究(B)
Authorship：Principal investigator
Teaching Experience (On-campus) 18
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ベーシックトレーニング
2024
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生物の化学
2024
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生物学基礎I
2024
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ベーシックトレーニング
2023
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生物の化学
2023
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生物学基礎I
2023
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生化学実習
2022
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生物の化学
2022
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生物学基礎I
2022
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ベーシックトレーニング
2022
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生物の化学
2021
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生化学実習
2021
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ベーシックトレーニング
2021
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生物学基礎I
2021
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ベーシックトレーニング
2020
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生化学実習
2020
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生物の化学
2020
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生物学基礎I
2020