Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1007/s10265-010-0319-4

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1242/dev.058891

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/aob/mcr137

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/j.1469-8137.2010.03535.x

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1104/pp.110.167502

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1371/journal.pone.0026162

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1094/MPMI-01-12-0011-R

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1038/ncomms1726

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/jxb/ers117

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pct029

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/pce.12097

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pct133

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/aob/mct305

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/tpj.12614

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/nph.12577

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/jxb/eru424

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/pcp/pcu209

PubMed

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/aob/mcv018

Language：English   Publishing type：Research paper (scientific journal)  

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1186/s12284-016-0124-3

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1111/pce.12849

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DOI： 10.3732/ajb.1600303

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Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1093/aob/mcx205

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PubMed

Language：English   Publisher：Plant, Cell and Environment  

Lack of O2 and high concentrations of iron (Fe) are common in flooded soils where Rice (Oryza sativa L.) is cultivated. We tested the hypothesis that growing in stagnant or high Fe conditions might induce the formation of apoplastic barriers in roots with different properties and chemical compositions. We measured radial O2 loss (ROL) from intact roots, the chemical composition of roots and the formation of root iron plaques in Fe-sensitive and Fe-tolerant rice genotypes grown in aerated, deoxygenated stagnant solutions or in aerated solutions containing high concentrations of Fe. Roots of plants grown in stagnant conditions developed tight barriers to ROL, while those grown in high Fe conditions developed only partial barriers. Chemical analysis of enzymatically isolated sclerenchyma/exodermal root cells indicated that roots grown in stagnant conditions showed increased amounts of suberin and glycerol esters. In contrast, roots in high Fe conditions showed an increase in suberization along with formation of iron plaques covering the roots. In high Fe conditions, exodermal suberization and the formation of partial ROL barriers were not influenced by the genotype's tolerance to Fe. The amount of O2 diffused from roots is influenced by the various layers that impede O2 diffusion. Specifically, increased amounts of glycerol esters in the suberized exodermis provide the greater resistance to ROL.

DOI： 10.1111/pce.15319

Scopus

PubMed

Language：English   Publisher：Theoretical and Applied Genetics  

Key message: We proposed models to predict the effects of genomic and environmental factors on daily soybean growth and applied them to soybean growth data obtained with unmanned aerial vehicles. Abstract: Advances in high-throughput phenotyping technology have made it possible to obtain time-series plant growth data in field trials, enabling genotype-by-environment interaction (G × E) modeling of plant growth. Although the reaction norm is an effective method for quantitatively evaluating G × E and has been implemented in genomic prediction models, no reaction norm models have been applied to plant growth data. Here, we propose a novel reaction norm model for plant growth using spline and random forest models, in which daily growth is explained by environmental factors one day prior. The proposed model was applied to soybean canopy area and height to evaluate the influence of drought stress levels. Changes in the canopy area and height of 198 cultivars were measured by remote sensing using unmanned aerial vehicles. Multiple drought stress levels were set as treatments, and their time-series soil moisture was measured. The models were evaluated using three cross-validation schemes. Although accuracy of the proposed models did not surpass that of single-trait genomic prediction, the results suggest that our model can capture G × E, especially the latter growth period for the random forest model. Also, significant variations in the G × E of the canopy height during the early growth period were visualized using the spline model. This result indicates the effectiveness of the proposed models on plant growth data and the possibility of revealing G × E in various growth stages in plant breeding by applying statistical or machine learning models to time-series phenotype data.

DOI： 10.1007/s00122-024-04565-5

Open Access

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PubMed

Language：English  

DOI： 10.3389/fpls.2022.1047563

Web of Science

PubMed

DOI： 10.1002/tpg2.20244

Web of Science

Language：English  

DOI： 10.3389/fpls.2022.828864

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PubMed

Language：Japanese  

DOI： 10.3389/fpls.2022.830577

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PubMed

Language：Japanese  

DOI： 10.1073/pnas.2101846119

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PubMed

Language：Japanese  

DOI： 10.1111/nph.16452

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PubMed

Language：Japanese  

DOI： 10.1270/jsbbs.20126

Web of Science

PubMed

Language：Japanese  

DOI： 10.17660/ActaHortic.2021.1309.4

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Language：Japanese  

DOI： 10.1093/aob/mcaa094

Web of Science

PubMed

DOI： 10.1080/1343943X.2020.1740600

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Oxford University Press (OUP)  

Abstract

CALCIUM-DEPENDENT PROTEIN KINASE (CDPK) stimulates reactive oxygen species (ROS)-dependent signaling by activating RESPIRATORY BURST OXIDASE HOMOLOG (RBOH). The lysigenous aerenchyma is a gas space created by cortical cell death that facilitates oxygen diffusion from the shoot to the root tips. Previously, we showed that RBOHH is indispensable for the induction of aerenchyma formation in rice (Oryza sativa) roots under low-oxygen conditions. Here, we showed that CDPK5 and CDPK13 localize to the plasma membrane where RBOHH functions. Mutation analysis of the serine at residues 92 and 107 of RBOHH revealed that these residues are required for CDPK5- and CDPK13-mediated activation of ROS production. The requirement of Ca2+ for CDPK5 and CDPK13 function was confirmed using in vitro kinase assays. CRISPR/Cas9-based mutagenesis of CDPK5 and/or CDPK13 revealed that the double knockout almost completely suppressed inducible aerenchyma formation, whereas the effects were limited in the single knockout of either CDPK5 or CDPK13. Interestingly, the double knockout almost suppressed the induction of adventitious root formation, which is widely conserved in vascular plants, under low-oxygen conditions. Our results suggest that CDPKs are essential for the acclimation of rice to low-oxygen conditions, and also for many other plant species conserving CDPK-targeted phosphorylation sites in RBOH homologues.

DOI： 10.1093/plphys/kiae293

Open Access

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

Rice (Oryza sativa L.) and many other wetland plants form an apoplastic barrier in the outer parts of the roots to restrict radial O2 loss to the rhizosphere during soil flooding. This barrier facilitates longitudinal internal O2 diffusion via gas-filled tissues from shoot to root apices, enabling root growth in anoxic soils. We tested the hypothesis that Leaf Gas Film 1 (LGF1), which influences leaf hydrophobicity in rice, plays a crucial role in tight outer apoplastic barriers formation in rice roots. We examined the roots of a rice mutant (dripping wet leaf 7, drp7) lacking functional LGF1, its wild type, and an LGF1 overexpression line for their capacity to develop outer apoplastic barriers that restrict radial O2 loss. We quantified the chemical composition of the outer part of the root and measured radial O2 diffusion from intact roots. The drp7 mutant exhibited a weak barrier to radial O2 loss compared to the wild type. However, introducing functional LGF1 into the mutant fully restored tight barrier function. The formation of a tight barrier to radial O2 loss was associated with increased glycerol ester levels in exodermal cells, rather than differences in total root suberization or lignification. These results demonstrate that, in addition to its role in leaf hydrophobicity regulation, LGF1 plays an important role in controlling the function of the outer apoplastic barriers in roots. Our study suggests that increased deposition of glycerol esters in the suberized root exodermis establishes a tight barrier to radial O2 loss in rice roots.

DOI： 10.1093/plphys/kiae458

Open Access

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

Nutritropism is a positive tropism toward nutrients in plant roots. An NH4+ gradient is a nutritropic stimulus in rice (Oryza sativa L.). When rice roots are exposed to an NH4+ gradient generated around nutrient sources, root tips bend toward and coil around the sources. The molecular mechanisms are largely unknown. Here, we analyzed the transcriptomes of the inside and outside of bending root tips exhibiting nutritropism to reveal nutritropic signal transduction. Tissues facing the nutrient sources (inside) and away (outside) were separately collected by laser microdissection. Principal component analysis revealed distinct transcriptome patterns between the two tissues. Annotations of 153 differentially expressed genes implied that auxin, gibberellin and ethylene signaling were activated differentially between the sides of the root tips under nutritropism. Exogenous application of transport and/or biosynthesis inhibitors of these phytohormones largely inhibited the nutritropism. Thus, signaling and de novo biosynthesis of the three phytohormones are necessary for nutritropism. Expression patterns of IAA genes implied that auxins accumulated more in the inside tissues, meaning that ammonium stimulus is transduced to auxin signaling in nutritropism similar to gravity stimulus in gravitropism. SAUR and expansin genes, which are known to control cell wall modification and to promote cell elongation in shoot gravitropism, were highly expressed in the inside tissues rather than the outside tissues, and our transcriptome data are unexplainable for differential elongation in root nutritropism.

DOI： 10.1093/pcp/pcae003

Open Access

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

Floods and droughts are becoming more frequent as a result of climate change and it is imperative to find ways to enhance the resilience of staple crops to abiotic stresses. This is crucial to sustain food production during unfavourable conditions. Here, we analyse the current knowledge about suberised and lignified outer apoplastic barriers, focusing on the functional roles of the barrier to radial O2 loss formed as a response to soil flooding and we discuss whether this trait also provides resilience to multiple abiotic stresses. The barrier is composed of suberin and lignin depositions in the exodermal and/or sclerenchyma cell walls. In addition to the important role during soil flooding, the barrier can also restrict radial water loss, prevent phytotoxin intrusion, salt intrusion and the main components of the barrier can impede invasion of pathogens in the root. However, more research is needed to fully unravel the induction pathway of the outer apoplastic barriers and to address potential trade-offs such as reduced nutrient or water uptake. Nevertheless, we suggest that the outer apoplastic barriers might act as a jack of all trades providing tolerance to multiple abiotic and/or biotic stressors.

DOI： 10.1071/FP23133

Open Access

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

Understanding how nutrient stress impacts plant growth is fundamentally important to the development of approaches to improve crop production under nutrient limitation. Here we applied single-cell RNA sequencing to shoot apices of Pisum sativum grown under boron (B) deficiency. We identified up to 15 cell clusters based on the clustering of gene expression profiles and verified cell identity with cell-type-specific marker gene expression. Different cell types responded differently to B deficiency. Specifically, the expression of photosynthetic genes in mesophyll cells (MCs) was down-regulated by B deficiency, consistent with impaired photosynthetic rate. Furthermore, the down-regulation of stomatal development genes in guard cells, including homologs of MUTE and TOO MANY MOUTHS, correlated with a decrease in stomatal density under B deficiency. We also constructed the developmental trajectory of the shoot apical meristem (SAM) cells and a transcription factor interaction network. The developmental progression of SAM to MC was characterized by up-regulation of genes encoding histones and chromatin assembly and remodeling proteins including homologs of FASCIATA1 (FAS1) and SWITCH DEFECTIVE/SUCROSE NON-FERMENTABLE (SWI/SNF) complex. However, B deficiency suppressed their expression, which helps to explain impaired SAM development under B deficiency. These results represent a major advance over bulk-tissue RNA-seq analysis in which cell-type-specific responses are lost and hence important physiological responses to B deficiency are missed. The reported findings reveal strategies by which plants adapt to B deficiency thus offering breeders a set of specific targets for genetic improvement. The reported approach and resources have potential applications well beyond P. sativum species and could be applied to various legumes to improve their adaptability to multiple nutrient or abiotic stresses.

DOI： 10.1111/tpj.16487

Open Access

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：American Association for the Advancement of Science (AAAS)  

DOI： 10.34133/plantphenomics.0244

Open Access

Scopus

PubMed

Publisher：Research Square Platform LLC  

Abstract

Advances in high-throughput phenotyping technology have made it possible to obtain time-series plant growth data in field trials, enabling genotype-by-environment interaction (G×E) modeling of plant growth. Although the reaction norm is an effective method for quantitatively evaluating G×E and has been implemented in genomic prediction models, no reaction norm models have been applied to plant growth data. Here, we propose a novel reaction norm model for plant growth using spline and random forest models, in which daily growth is explained by environmental factors one day prior. The proposed model was applied to soybean canopy area and height to evaluate the influence of drought stress levels. Changes in the canopy area and height of 198 cultivars were measured by remote sensing using unmanned aerial vehicles. Multiple drought stress levels were set as treatments and their time-series soil moisture was measured. The models were evaluated using leave-one-environment-out cross-validation, in which a treatment-by-year combination was considered the environment. These results suggest that our model can capture G×E during the early growth, especially canopy height. Significant variations in the G×E of the canopy height during the early growth period were visualized using the estimated reaction norms. This result indicates the effectiveness of the proposed models on plant growth data and the possibility of revealing G×E in various growth stages in plant breeding by applying statistical or machine learning models to time-series phenotype data obtained with remote sensing.

DOI： 10.21203/rs.3.rs-3267332/v1

Other Link： https://www.researchsquare.com/article/rs-3267332/v1.html

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Research Square Platform LLC  

Abstract

Aerenchymatous phellem (AP) is important for internal aeration and adaptation to waterlogging in plants. Herein, the extensive accumulation of triterpenoids such as lupeol and betulinic acid was identified in AP. However, the biological and physiological roles of these triterpenoids in plants are largely unknown. Lupeol is converted from 2,3-oxidosqualene by lupeol synthase (LUS) and oxidized to betulinic acid. Functional analysis of LUS genes in soybean revealed that GmLUS1 is crucial for triterpenoid biosynthesis in AP. Lupeol and betulinic acid were found to be the major components of epicuticular wax on the surface of AP cells, and they contributed to tissue hydrophobicity and oxygen transport to roots. Additionally, the lus1 mutant produced a shallow root system due to less oxygen transport via AP under waterlogged conditions. In conclusion, triterpenoid accumulation in AP aids internal aeration and root development for adaptation to waterlogging.

DOI： 10.1111/nph.18975

Scopus

PubMed

Other Link： https://www.researchsquare.com/article/rs-2230730/v1.html

Language：English   Publishing type：Research paper (scientific journal)  

The root barrier to radial O2 loss (ROL) is a key root trait preventing O2 loss from roots to anoxic soils, thereby enabling root growth into anoxic, flooded soils. We hypothesized that the ROL barrier can also prevent intrusion of hydrogen sulphide (H2 S), a potent phytotoxin in flooded soils. Using H2 S- and O2 -sensitive microsensors, we measured the apparent permeance to H2 S of rice roots, tested whether restricted H2 S intrusion reduced its adverse effects on root respiration, and whether H2 S could induce the formation of a ROL barrier. The ROL barrier reduced apparent permeance to H2 S by almost 99%, greatly restricting H2 S intrusion. The ROL barrier acted as a shield towards H2 S; O2 consumption in roots with a ROL barrier remained unaffected at high H2 S concentration (500 μM), compared to a 67% decline in roots without a barrier. Importantly, low H2 S concentrations induced the formation of a ROL barrier. In conclusion, the ROL barrier plays a key role in protecting against H2 S intrusion, and H2 S can act as an environmental signalling molecule for the induction of the barrier. This study demonstrates the multiple functions of the suberized/lignified outer part of the rice root beyond that of restricting ROL.

DOI： 10.1111/nph.18883

Open Access

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Plant Biotechnology  

<p>In plants, mitogen activated protein kinases (MPKs) are involved in various signaling pathways that lead to biotic and abiotic responses as well as that regulate developmental processes. Among them, MPK6 and its closely related homologue, MPK3, act redundantly and are known to be involved in asymmetric cell divisions of meristemoid mother cells in stomata development and of zygotes in <i>Arabidopsis</i>. Loss-of-function mutants of <i>GLE4</i>/<i>OsMPK6</i>, which is an orthologue of <i>MPK6</i> in rice, showed a defect in polarity establishment in early stage of embryogenesis. However, because of the embryo lethality of the mutations, the function of GLE4/OsMPK6 in post-embryonic development is not clarified. Here, we report the analysis of post embryonic function of GLE4/OsMPK6 in vegetative stage of rice using regenerated <i>gle4</i>/<i>osmpk6</i> homozygous plants from tissue culture. The regenerated plants are dwarf and produce multiple shoots with small leaves. These shoots never develop into reproductive stage, instead, proliferate vegetative shoots repeatedly. Leaves of <i>gle4</i>/<i>osmpk6</i> have small leaf blade at the tip and blade-sheath boundary become obscure. Stomata arrangement is also disturbed in <i>gle4</i>/<i>osmpk6</i> leaf blade. The shape of shoot apical meristem of <i>gle4</i>/<i>osmpk6</i> become disorganized. Thus, GLE4/OsMPK6 functions in shoot organization and stomata patterning in the post embryonic development in rice.</p>

DOI： 10.5511/plantbiotechnology.22.1117a

Open Access

Scopus

PubMed

CiNii Research

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Frontiers Media SA  

Plant response to drought is an important yield-related trait under abiotic stress, but the method for measuring and modeling plant responses in a time series has not been fully established. The objective of this study was to develop a method to measure and model plant response to irrigation changes using time-series multispectral (MS) data. We evaluated 178 soybean (Glycine max (L.) Merr.) accessions under three irrigation treatments at the Arid Land Research Center, Tottori University, Japan in 2019, 2020 and 2021. The irrigation treatments included W5: watering for 5 d followed by no watering 5 d, W10: watering for 10 d followed by no watering 10 d, D10: no watering for 10 d followed by watering 10 d, and D: no watering. To capture the plant responses to irrigation changes, time-series MS data were collected by unmanned aerial vehicle during the irrigation/non-irrigation switch of each irrigation treatment. We built a random regression model (RRM) for each of combination of treatment by year using the time-series MS data. To test the accuracy of the information captured by RRM, we evaluated the coefficient of variation (CV) of fresh shoot weight of all accessions under a total of nine different drought conditions as an indicator of plant’s stability under drought stresses. We built a genomic prediction model () using the genetic random regression coefficients of RRM as secondary traits and evaluated the accuracy of each model for predicting CV. In 2020 and 2021,the mean prediction accuracies of built in the changing irrigation treatments (r = 0.44 and 0.49, respectively) were higher than that in the continuous drought treatment (r = 0.34 and 0.44, respectively) in the same year. When the CV was predicted using the across 2020 and 2021 in the changing irrigation treatment, the mean prediction accuracy (r = 0.46) was 42% higher than that of the simple genomic prediction model (r =0.32). The results suggest that this RRM method using the time-series MS data can effectively capture the genetic variation of plant response to drought.

DOI： 10.3389/fpls.2023.1201806

Open Access

Scopus

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.3389/fpls.2023.1133009

Open Access

Scopus

PubMed

DOI： 10.1101/2022.11.16.516519

Authorship：Lead author,　Corresponding author   Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1270/jsbbs.20126

Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.3117/plantroot.14.33

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Root Research  

<p>The radial oxygen loss (ROL) barrier formed on the outer cell layers of roots of rice (<i>Oryza sativa</i>) contributes to efficient oxygen transfer through the aerenchyma from the aerial parts to the root apex. It is hypothesized that suberin accumulation in the exodermis contributes to an apoplastic barrier and plays a vital role in ROL barrier formation. A previous study reported that some genes encoding cytochrome P450 family 86 (<i>CYP86</i>) and glycerol-3-phosphate acyltransferase (<i>GPAT</i>) might be involved in suberin biosynthesis during ROL barrier development of rice roots. However, how these genes are expressed and their contribution to the sequential development of suberin accumulation in rice roots remains unclear. In this study, four <i>CYP86</i> and five <i>GPAT</i> genes of rice were identified as candidate genes involved in suberin biosynthesis in roots using sequence homology alignment with <i>Arabidopsis</i> suberin and cutin biosynthesis genes. Gene expression analyses revealed that expression of the candidate genes was induced at the region where suberin biosynthesis occurred under stagnant deoxygenated conditions. These genes showed two types of spatiotemporal expression patterns, at the regions of 5–25 mm and 25–35 mm from the root apex. Tissue-specific expression analyses using laser microdissection and histochemical GUS staining revealed that candidate gene expression was similar in the cell layer of the root exodermis. These results suggest that the selected <i>CYP86</i> and <i>GPAT</i> genes are involved in suberin biosynthesis in the exodermis, and that suberin biosynthesis in the root may be controlled by the spatiotemporal expression of two groups of genes.</p>

DOI： 10.3117/plantroot.15.19

Open Access

Web of Science

Scopus

CiNii Research

Publishing type：Research paper (scientific journal)   Publisher：Springer Science and Business Media LLC  

DOI： 10.1007/s41207-020-00209-4

Web of Science

Other Link： http://link.springer.com/article/10.1007/s41207-020-00209-4/fulltext.html

Language：English   Publishing type：Research paper (scientific journal)  

DOI： https://doi.org/10.1080/1343943X.2020.1740600

DOI： 10.2503/hortj.UTD-126

Web of Science

Language：English   Publishing type：Research paper (scientific journal)   Publisher：Japanese Society for Root Research  

<p>A barrier to restrict radial oxygen loss (ROL) promotes the adaptation of plants to waterlogged soil conditions. A ROL barrier is formed in the basal parts of roots and contributes to the enhancement of the longitudinal diffusion of oxygen (O₂) via aerenchyma toward the root tips. The adventitious roots of rice (<i>Oryza sativa</i>) can form a ROL barrier in waterlogged soil; however, whether a ROL barrier can be formed in the lateral roots (LRs) of rice remains unclear. Rice possesses two types of LRs: L- and S-type. L-type LRs are generally long, thick, and capable of branching, whereas S-type LRs are short, thin, and incapable of branching. In this study, we examined whether the L- and S-type LRs of rice possess the ability to form ROL barriers. In L-type LRs, a ROL barrier was formed; the aerenchyma, which is constitutively formed under aerated conditions, was further developed under stagnant deoxygenated conditions, suggesting that these traits promote the supply of O₂ to the root tips of LRs. However, neither a ROL barrier, nor aerenchyma was formed in S-type LRs, and thus ROL was observed mostly in the basal parts. Patterns of deposition of suberin, which is thought to be one of the components of the ROL barrier, were consistent with the patterns of ROL-barrier formation in L-type and S-type LRs. These results suggest that L- and S-type LRs play distinct roles in the growth of rice plants in waterlogged soils and in oxygenating the rhizosphere.</p>

DOI： 10.3117/plantroot.14.33

Open Access

Web of Science

Scopus

CiNii Research

DOI： 10.1093/jxb/erz403

Web of Science

PubMed

DOI： 10.1111/pce.13562

Web of Science

PubMed

DOI： 10.1242/dev.176305

PubMed

DOI： 10.17660/ActaHortic.2017.1156.13

Web of Science

Language：English  

DOI： 10.1186/1939-8433-5-2

PubMed

Language：Japanese  

Language：English  

DOI： 10.1093/pcp/pcl049

PubMed

Language：Japanese  

高等植物は機能の異なる数十種類の細胞より構成されている．個々の細胞・組織の役割について理解するためには，それらの細胞・組織を純粋に単離して，DNA， RNA，タンパク質，代謝産物等を抽出し，解析する必要がある．しかし，これまでの遺伝子発現解析などの研究は，主に葉，根，茎，花といった器官レベルで行われてきた．器官レベルの解析では，特定の細胞・組織で機能する遺伝子を見逃す可能性があったが，近年，標的細胞・組織のみを単離することができるレーザーマイクロダイセクションの植物への適用が実現したことにより，細胞・組織レベルでの精度の高い遺伝子発現解析が可能となった．本稿では，このレーザーマイクロダイセクション法の概略について紹介する．

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：京都産業大学   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京農業大学   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東北大学   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：滋賀県雄琴温泉   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：滋賀県雄琴温泉   Country：Japan  

Language：Japanese   Presentation type：Poster presentation  

Venue：拓殖大学   Country：Japan  

Language：Japanese   Presentation type：Poster presentation  

Venue：愛媛大学   Country：Japan  

Language：Japanese   Presentation type：Poster presentation  

Venue：愛媛大学   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：つくば   Country：Japan  

Language：Japanese   Presentation type：Poster presentation  

Venue：畜産草地研究所   Country：Japan  

Language：Japanese   Presentation type：Poster presentation  

Venue：東京大学   Country：Japan  

Language：Japanese   Presentation type：Poster presentation  

Venue：東京大学   Country：Japan  

Language：Japanese   Presentation type：Poster presentation  

Venue：横浜市立大学   Country：Japan  

Language：Japanese   Presentation type：Poster presentation  

Venue：日本橋ライフサイエンスハブ   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：福井県立大学   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：三重大学   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：横浜市立大学   Country：Japan  

Language：English   Presentation type：Poster presentation  

Country：Japan  

Language：English   Presentation type：Poster presentation  

Country：United States  

Language：English   Presentation type：Poster presentation  

Country：Italy  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：愛媛大学   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：茨城大学   Country：Japan  

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：明治大学   Country：Japan  

Language：English   Presentation type：Oral presentation (general)  

Country：Philippines  

Language：English   Presentation type：Oral presentation (general)  

Country：Australia