Updated on 2021/10/25

写真a

 
MURAKAMI Hiroshi
 
Organization
Graduate School of Engineering Biomolecular Engineering 1 Professor
Graduate School
Graduate School of Engineering
Undergraduate School
School of Engineering Chemistry and Biotechnology
Title
Professor
Contact information
メールアドレス

Degree 1

  1. 博士(工学) ( 2000.3   岡山大学 ) 

Research Interests 11

  1. Antibody-like protein

  2. リボソーム

  3. 遺伝暗号

  4. 非天然アミノ酸

  5. ペプチド

  6. タンパク質

  7. 翻訳反応

  8. 翻訳反応

  9. 遺伝暗号

  10. リボソーム

  11. Antibody-like protein

Current Research Project and SDGs 3

  1. Development of in vitro selection method for obtaining antibody-like proteins

  2. Development of anti-SARS-CoV-2 antibody-like proteins

  3. Development of mutant ribosomes for incorporating various non-natural amino acids

Research History 6

  1. Nagoya University   Institute of Innovation for Future Society

    2019.4

  2. Professor, Nagoya University

    2015.4

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    Country:Japan

  3. Associate Professor, The University of Tokyo

    2009.4 - 2015.3

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    Country:Japan

  4. Associate Professor, The University of Tokyo

    2009.4 - 2015.3

  5. Assistant professor, The University of Tokyo

    2003.4 - 2009.3

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    Country:Japan

  6. Assistant professor, The University of Tokyo

    2003.4 - 2009.3

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Education 2

  1. Okayama University   Graduate School, Division of Science and Technology

    1997.4 - 2000.3

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    Country: Japan

  2. Okayama University   Graduate School, Division of Engineering

    1995.4 - 1997.3

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    Country: Japan

Professional Memberships 8

  1. 日本RNA学会

  2. 日本分子生物学会

  3. 日本ペプチド学会

  4. アメリカ化学会

  5. 日本化学会

  6. 日本ペプチド学会

  7. 日本分子生物学会

  8. 日本化学会

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Committee Memberships 1

  1. 日本化学会 生体機能関連部会   幹事  

    2017.9   

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    Committee type:Academic society

Awards 1

  1. 第13回(平成28年度)日本学術振興会賞

    2016.2   独立行政法人日本学術振興会   非天然アミノ酸を含むペプチドの翻訳合成

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    Country:Japan

 

Papers 68

  1. cDNA TRAP display for rapid and stable in vitro selection of antibody-like proteins. International journal

    Taishi Kondo, Minori Eguchi, Seita Kito, Tomoshige Fujino, Gosuke Hayashi, Hiroshi Murakami

    Chemical communications (Cambridge, England)   Vol. 57 ( 19 ) page: 2416 - 2419   2021.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    We developed a cDNA TRAP display for the rapid selection of antibody-like proteins in various conditions. By modifying the original puromycin linker in the TRAP display, a monobody was covalently attached to the cDNA. As a proof-of-concept, we demonstrated a rapid model selection of an anti-EGFR1 monobody in a solution containing ribonuclease.

    DOI: 10.1039/d0cc07541h

    PubMed

  2. Antibody-like proteins that capture and neutralize SARS-CoV-2. Reviewed

    Kondo, T.; Iwatani, Y.; Matsuoka, K.; Fujino, T.; Umemoto, S.; Yokomaku, Y.; Ishizaki, K.; Kito, S.; Sezaki, T.; Hayashi, G.; Murakami, H.*

      Vol. 6 ( 42 ) page: eabd3916   2020.10

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  3. An amino acid-swapped genetic code. Reviewed

    Fujino, T.; Tozaki, M.; Murakami, H.*

    ACS Synthetic Biology   Vol. 9 ( 10 ) page: 2703 - 2713   2020.9

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  4. Ribosomal Synthesis of Peptides with Multiple β-Amino Acids. Reviewed

    Fujino T, Goto Y, Suga H, Murakami H

    Journal of the American Chemical Society   Vol. 138 ( 6 ) page: 1962-1969   2016.2

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/jacs.5b12482

    PubMed

  5. TRAP Display: A High-Speed Selection Method for the Generation of Functional Polypeptides Reviewed

    Takahiro Ishizawa, Takashi Kawakami, Patrick C. Reid, Hiroshi Murakami

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   Vol. 135 ( 14 ) page: 5433 - 5440   2013.4

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:AMER CHEMICAL SOC  

    Here, we describe a novel method that enables highspeed in vitro selection of functional peptides, peptidomimetics, and proteins via a simple procedure. We first developed a new cell-free translation system, the TRAP system (transcription translation coupled with association of Euromycin linker), which automatically produces a polypeptide library through a series of sequential reactions: transcription, association of puromycin-DNA linker, translation, and conjugation between the nascent polypeptide and puromycin-DNA linker. We then applied the TRAP system for the selection of macrocyclic peptides against human serum albumin. Six rounds of selection using TRAP display were performed in approximately 14 h, yielding macrocyclic peptides with nanomolar affinity to their target protein. Because TRAP display enables high-speed selection of functional polypeptides, it will facilitate the generation of various polypeptides that are useful for biological and therapeutic applications.

    DOI: 10.1021/ja312579u

    Web of Science

    PubMed

  6. Construction of a Highly Diverse mRNA Library for in vitro Selection of Monobodies.

    Kondo T, Eguchi M, Tsuzuki N, Murata N, Fujino T, Hayashi G, Murakami H

    Bio-protocol   Vol. 11 ( 16 ) page: e4125   2021.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.21769/BioProtoc.4125

    PubMed

  7. Exploring the Minimal RNA Substrate of Flexizymes. Reviewed

    Fujino, T.; Kondo, T.; Suga, H.; Murakami, H.

    Chembiochem   Vol. 20 ( 15 ) page: 1959-1965   2019.4

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1002/cbic.201900150

  8. Multiple Site-Directed and Saturation Mutagenesis by the Patch Cloning Method. Reviewed

    Naohiro Taniguchi, Hiroshi Murakami

    Methods Mol Biol   Vol. 1498   page: 339-347   2017

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/978-1-4939-6472-7_22

  9. Expanding the amino acid repertoire of ribosomal polypeptide synthesis via the artificial division of codon boxes. Reviewed

    Iwane Y, Hitomi A, Murakami H, Katoh T, Goto Y, Suga H

    Nature chemistry   Vol. 8 ( 4 ) page: 317-325   2016.4

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/nchem.2446

    PubMed

  10. In Vitro Selection Combined with Ribosomal Translation Containing Non-proteinogenic Amino Acids. Reviewed

    Fujino T, Murakami H

    Chemical record (New York, N.Y.)   Vol. 16 ( 1 ) page: 365-377   2016.2

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/tcr.201500239

    PubMed

  11. Incorporation of electrically charged N-alkyl amino acids into ribosomally synthesized peptides via post-translational conversion. Reviewed

    Kawakami, T.*; Sasaki, T; Reid, P. C.; Murakami, H.

    Chemical Science   Vol. 5   page: 887-893   2014.11

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1039/C3SC52744A

  12. ZFP36L1 and ZFP36L2 control LDLR mRNA stability via the ERK-RSK pathway. Reviewed

    Adachi S, Homoto M, Tanaka R, Hioki Y, Murakami H, Suga H, Matsumoto M, Nakayama KI, Hatta T, Iemura S, Natsume T

    Nucleic acids research   Vol. 42 ( 15 ) page: 10037-10049   2014.9

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nar/gku652

    PubMed

  13. Nonstandard peptide expression under the genetic code consisting of reprogrammed dual sense codons. Reviewed

    Goto Y, Iseki M, Hitomi A, Murakami H, Suga H

    ACS chemical biology   Vol. 8 ( 12 ) page: 2630-2634   2013.12

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/cb400549p

    PubMed

  14. Reevaluation of the D-amino acid compatibility with the elongation event in translation. Reviewed

    Fujino T, Goto Y, Suga H, Murakami H

    Journal of the American Chemical Society   Vol. 135 ( 5 ) page: 1830-1837   2013.2

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/ja309570x

    PubMed

  15. In vitro selection of multiple libraries created by genetic code reprogramming to discover macrocyclic peptides that antagonize VEGFR2 activity in living cells. Reviewed

    Kawakami T, Ishizawa T, Fujino T, Reid PC, Suga H, Murakami H

    ACS chemical biology   Vol. 8 ( 6 ) page: 1205-1214   2013

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/cb300697h

    PubMed

  16. Patch cloning method for multiple site-directed and saturation mutagenesis Reviewed

    Taniguchi, N; Nakayama, S; Kawakami, T.; Murakami, H.

    BMC Biotechnology     page: 13:91   2013

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  17. Extensive reprogramming of the genetic code for genetically encoded synthesis of highly N-alkylated polycyclic peptidomimetics. Reviewed

    Kawakami, T.; Ishizawa, T.; Murakami, H

    Journal of the American Chemical Society   Vol. 135   page: 12297-304   2013

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    Language:English   Publishing type:Research paper (scientific journal)  

  18. Diverse backbone-cyclized peptides via codon reprogramming. Reviewed

    Kawakami T, Ohta A, Ohuchi M, Ashigai H, Murakami H, Suga H

    Nature chemical biology   Vol. 5 ( 12 ) page: 888-890   2009.12

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/nchembio.259

    PubMed

  19. A flexizyme that selectively charges amino acids activated by a water-friendly leaving group. Reviewed

    Niwa N, Yamagishi Y, Murakami H, Suga H

    Bioorganic & medicinal chemistry letters   Vol. 19 ( 14 ) page: 3892-3894   2009.7

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bmcl.2009.03.114

    PubMed

  20. Ribosomal synthesis of cyclic peptides with a fluorogenic oxidative coupling reaction. Reviewed

    Yamagishi Y, Ashigai H, Goto Y, Murakami H, Suga H

    Chembiochem : a European journal of chemical biology   Vol. 10 ( 9 ) page: 1469-1472   2009.6

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cbic.200900021

    PubMed

  21. Ribosomal synthesis of dehydrobutyrine- and methyllanthionine-containing peptides. Reviewed

    Goto Y, Iwasaki K, Torikai K, Murakami H, Suga H

    Chemical communications (Cambridge, England)   ( 23 ) page: 3419-3421   2009.6

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1039/b904314d

    PubMed

  22. Ribosomal synthesis of peptides with C-terminal lactams, thiolactones, and alkylamides. Reviewed

    Nakajima E, Goto Y, Sako Y, Murakami H, Suga H

    Chembiochem : a European journal of chemical biology   Vol. 10 ( 7 ) page: 1186-1192   2009.5

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cbic.200900058

    PubMed

  23. Bases in the anticodon loop of tRNA(Ala)(GGC) prevent misreading. Reviewed

    Murakami H, Ohta A, Suga H

    Nature structural & molecular biology   Vol. 16 ( 4 ) page: 353-358   2009.4

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/nsmb.1580

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  24. Ribosomal synthesis of polypeptoids and peptoid-peptide hybrids. Reviewed

    Kawakami T, Murakami H, Suga H

    Journal of the American Chemical Society   Vol. 130 ( 50 ) page: 16861-16863   2008.12

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/ja806998v

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  25. Polymerization of alpha-hydroxy acids by ribosomes. Reviewed

    Ohta A, Murakami H, Suga H

    Chembiochem : a European journal of chemical biology   Vol. 9 ( 17 ) page: 2773-2778   2008.11

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cbic.200800439

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  26. Initiating translation with D-amino acids. Reviewed

    Goto Y, Murakami H, Suga H

    RNA (New York, N.Y.)   Vol. 14 ( 7 ) page: 1390-1398   2008.7

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1261/rna.1020708

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  27. Structural basis of specific tRNA aminoacylation by a small in vitro selected ribozyme. Reviewed

    Xiao H, Murakami H, Suga H, Ferré-D'Amaré AR

    Nature   Vol. 454 ( 7202 ) page: 358-361   2008.7

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/nature07033

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  28. Ribosomal synthesis of bicyclic peptides via two orthogonal inter-side-chain reactions. Reviewed

    Sako Y, Morimoto J, Murakami H, Suga H

    Journal of the American Chemical Society   Vol. 130 ( 23 ) page: 7232-7234   2008.6

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/ja800953c

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  29. Ribosomal synthesis of peptidase-resistant peptides closed by a nonreducible inter-side-chain bond. Reviewed

    Sako Y, Goto Y, Murakami H, Suga H

    ACS chemical biology   Vol. 3 ( 4 ) page: 241-249   2008.4

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/cb800010p

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  30. Reprogramming the translation initiation for the synthesis of physiologically stable cyclic peptides. Reviewed

    Goto Y, Ohta A, Sako Y, Yamagishi Y, Murakami H, Suga H

    ACS chemical biology   Vol. 3 ( 2 ) page: 120-129   2008.2

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/cb700233t

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  31. Messenger RNA-programmed incorporation of multiple N-methyl-amino acids into linear and cyclic peptides. Reviewed

    Kawakami T, Murakami H, Suga H

    Chemistry & biology   Vol. 15 ( 1 ) page: 32-42   2008.1

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.chembiol.2007.12.008

    PubMed

  32. Synthesis of polyester by means of genetic code reprogramming. Reviewed

    Ohta A, Murakami H, Higashimura E, Suga H

    Chemistry & biology   Vol. 14 ( 12 ) page: 1315-1322   2007.12

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.chembiol.2007.10.015

    PubMed

  33. The flexizyme system: a highly flexible tRNA aminoacylation tool for the translation apparatus. Reviewed

    Ohuchi M, Murakami H, Suga H

    Current opinion in chemical biology   Vol. 11 ( 5 ) page: 537-542   2007.10

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.cbpa.2007.08.011

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  34. [Reprogramming the genetic code using flexizyme]. Reviewed

    Sako Y, Murakami H, Suga H

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   Vol. 52 ( 13 Suppl ) page: 1643-1648   2007.10

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    PubMed

  35. Ribosomal Polyester Synthesis Using Reprogrammed Genetic Code Reviewed

    Atsushi Ohta, Hiroshi Murakami, Hiroaki Suga

    Kobunshi   Vol. 56 ( 4 ) page: 196 - 199   2007

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    Language:English   Publishing type:Research paper (scientific journal)  

    The translation apparatus is sophisticated machinery that polymerizes amino acids to polypeptide according to the genetic triplets encoded on messenger RNA (mRNA) sequence. Because of this property, ribosome generally acts as a superb template-directed polymer synthesizer and therefore translated polypeptide shows narrow distribution of molecular weights with complete controlled sequence. Unfortunately, this superior system is limited to polypeptide synthesis because of the lack of genetic code which converts genetic triplets to any monomers except for 20 natural amino acids. To expand the application of ribosome, we utilized the concept of genetic code reprogramming to assign several codons as hydroxy acids and performed mRNA-templated polyester synthesis. We have demonstrated the sequence and length of polyesters can be controlled by the mRNA sequence as designed. This is a novel technology for the synthesis of polyester and polyester-polypeptide copolymer for the investigation of new materials. © 2007, The Society of Polymer Science, Japan. All rights reserved.

    DOI: 10.1295/kobunshi.56.196

    Scopus

  36. In situ generation of aminoacyl-tRNAs assisted by ribozymes in translation apparatus. Reviewed

    Ohuchi M, Murakami H, Suga H

    Nucleic acids symposium series (2004)   ( 51 ) page: 115-116   2007

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nass/nrm058

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  37. Exploration of incorporation of Nalpha-methylated amino acids into peptides by sense-suppression method. Reviewed

    Kawakami T, Murakami H, Suga H

    Nucleic acids symposium series (2004)   ( 51 ) page: 361-362   2007

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nass/nrm181

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  38. [Expansion and reprogramming of genetic code by Flexizyme]. Reviewed

    Murakami H, Suga H

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   Vol. 51 ( 16 Suppl ) page: 2496-2501   2006.12

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    PubMed

  39. フレキシザイムを用いた遺伝暗号の拡張とリプログラミング (RNAと生命) -- (RNAテクノロジーと創薬)

    村上 裕, 菅 裕明

    蛋白質核酸酵素   Vol. 51 ( 16 ) page: 2496-2501   2006.12

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    CiNii Article

  40. Leucyl/Phenylalanyl-tRNA-protein transferase-mediated chemoenzymatic coupling of N-terminal Arg/Lys units in post-translationally processed proteins with non-natural amino acids. Reviewed

    Taki M, Kuno A, Matoba S, Kobayashi Y, Futami J, Murakami H, Suga H, Taira K, Hasegawa T, Sisido M

    Chembiochem : a European journal of chemical biology   Vol. 7 ( 11 ) page: 1676-1679   2006.11

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cbic.200600181

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  41. テクノ・トレンド フレキシザイム--遺伝暗号の拡張とリプログラミングへの応用

    村上 裕, 菅 裕明

    バイオテクノロジージャーナル   Vol. 6 ( 6 ) page: 734-737   2006.11

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    CiNii Article

  42. A highly flexible tRNA acylation method for non-natural polypeptide synthesis. Reviewed

    Murakami H, Ohta A, Ashigai H, Suga H

    Nature methods   Vol. 3 ( 5 ) page: 357-359   2006.5

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/nmeth877

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  43. セントラルドグマをつくりかえる (特集 生命をデザインする合成生物学)

    村上 裕, 菅 裕明, 平尾 一郎

    バイオニクス   Vol. 3 ( 3 ) page: 34-39   2006.3

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    CiNii Article

  44. Flexizyme as a versatile tRNA acylation catalyst and the application for translation. Reviewed

    Murakami H, Ohta A, Goto Y, Sako Y, Suga H

    Nucleic acids symposium series (2004)   ( 50 ) page: 35-36   2006

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nass/nrl018

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  45. In vitro evolution of flexizymes that function under the conditions in translation system. Reviewed

    Ohuchi M, Murakami H, Suga H

    Nucleic acids symposium series (2004)   ( 50 ) page: 299-300   2006

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nass/nrl149

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  46. Translation initiation by using various N-acylaminoacyl tRNAs. Reviewed

    Goto Y, Ashigai H, Sako Y, Murakami H, Suga H

    Nucleic acids symposium series (2004)   ( 50 ) page: 293-294   2006

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nass/nrl146

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  47. Programmable ribozymes for mischarging tRNA with nonnatural amino acids and their applications to translation. Reviewed

    Kourouklis D, Murakami H, Suga H

    Methods (San Diego, Calif.)   Vol. 36 ( 3 ) page: 239-244   2005.7

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.ymeth.2005.04.001

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  48. Designer ribozymes: programming the tRNA specificity into flexizyme. Reviewed

    Ramaswamy K, Saito H, Murakami H, Shiba K, Suga H

    Journal of the American Chemical Society   Vol. 126 ( 37 ) page: 11454-11455   2004.9

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/ja046843y

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  49. Position-specific incorporation of dansylated non-natural amino acids into streptavidin by using a four-base codon. Reviewed

    Hohsaka T, Muranaka N, Komiyama C, Matsui K, Takaura S, Abe R, Murakami H, Sisido M

    FEBS letters   Vol. 560 ( 1-3 ) page: 173-177   2004.2

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/S0014-5793(04)00099-7

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  50. Using a solid-phase ribozyme aminoacylation system to reprogram the genetic code. Reviewed

    Murakami H, Kourouklis D, Suga H

    Chemistry & biology   Vol. 10 ( 11 ) page: 1077-1084   2003.11

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  51. [Artificial ribozymes: selection and application for nonnatural amino acid mutagenesis]. Reviewed

    Murakami H, Suga H

    Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme   Vol. 48 ( 11 Suppl ) page: 1511-1518   2003.8

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  52. 人工リボザイム--試験管内分子進化と非天然アミノ酸変異法への応用 (化学と生物学の接点がつくるNewバイオテクノロジー) -- (新規遺伝子と蛋白質を創製する)

    村上 裕, 菅 裕明

    蛋白質核酸酵素   Vol. 48 ( 11 ) page: 1511-1518   2003.8

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    CiNii Article

  53. A versatile tRNA aminoacylation catalyst based on RNA. Reviewed

    Murakami H, Saito H, Suga H

    Chemistry & biology   Vol. 10 ( 7 ) page: 655-662   2003.7

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  54. Random insertion and deletion mutagenesis. Reviewed

    Murakami H, Hohsaka T, Sisido M

    Methods in molecular biology (Clifton, N.J.)   Vol. 231   page: 53-64   2003

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    DOI: 10.1385/1-59259-395-X:53

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  55. Position-specific incorporation of a fluorophore-quencher pair into a single streptavidin through orthogonal four-base codon/anticodon pairs. Reviewed

    Taki M, Hohsaka T, Murakami H, Taira K, Sisido M

    Journal of the American Chemical Society   Vol. 124 ( 49 ) page: 14586-14590   2002.12

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  56. Aminoacyl-tRNA synthesis by a resin-immobilized ribozyme. Reviewed

    Murakami H, Bonzagni NJ, Suga H

    Journal of the American Chemical Society   Vol. 124 ( 24 ) page: 6834-6835   2002.6

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  57. Random insertion and deletion of arbitrary number of bases for codon-based random mutation of DNAs. Reviewed

    Murakami H, Hohsaka T, Sisido M

    Nature biotechnology   Vol. 20 ( 1 ) page: 76-81   2002.1

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    Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/nbt0102-76

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  58. A novel fluorescent nonnatural amino acid that can be incorporated into a specific position of streptavidin. Reviewed

    Taki M, Hohsaka T, Murakami H, Kuno A, Hasegawa T, Taira K, Sisido M

    Nucleic acids research. Supplement (2001)   ( 2 ) page: 203-204   2002

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  59. A non-natural amino acid for efficient incorporation into proteins as a sensitive fluorescent probe

    Taki, M. and Hohsaka, T. and Murakami, H. and Taira, K. and Sisido, M.

    FEBS Letters   Vol. 507 ( 1 ) page: 35-38   2001

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    Language:English   Publishing type:Research paper (scientific journal)  

  60. Five-base codons for incorporation of nonnatural amino acids into proteins

    Hohsaka, T. and Ashizuka, Y. and Murakami, H. and Sisido, M.

    Nucleic Acids Res.   Vol. 29 ( 17 ) page: 3646-3651   2001

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    Language:English   Publishing type:Research paper (scientific journal)  

  61. Incorporation of nonnatural amino acids into proteins by using various four-base codons in an Escherichia coli in vitro translation system

    Hohsaka, T. and Ashizuka, Y. and Taira, H. and Murakami, H. and Sisido, M.

    Biochemistry   Vol. 40 ( 37 ) page: 11060-11064   2001

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  62. Site-directed incorporation of fluorescent nonnatural amino acids into streptavidin for highly sensitive detection of biotin

    Hiroshi Murakami, Takahiro Hohsaka, Yuki Ashizuka, Kimiko Hashimoto, Masahiko Sisido

    Biomacromolecules   Vol. 1 ( 1 ) page: 118 - 125   2000

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Chemical Society  

    Fluorescent nonnatural amino acids were incorporated into specific positions of streptavidin. The positions of the nonnatural amino acids were directed by a CGGG/CCCG four-base codon/anticodon pair. The nonnatural mutants with a single 2-anthrylalanine at the 22nd, 43rd, 54th, and 120th positions, respectively, were found to bind biotin, indicating that the mutants retained active conformation. The fluorescence intensities of the anthryl groups were relatively insensitive to the positions and the biotin binding when excited at 265 nm. When the anthryl group at the 120th position was excited through energy transfer from tryptophan units, the fluorescence intensity markedly decreased with biotin binding, because of a suppression of the energy transfer. Amino acids carrying 7-methoxycoumarine fluorophore were also incorporated at the 120th position Their fluorescence quantum yields were very sensitive to the biotin binding. The high sensitivity of the coumarine-labeled streptavidin exemplifies potential applications of fluorescent nonnatural mutants for detecting specific molecules at very low concentrations.

    DOI: 10.1021/bm990012g

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    PubMed

  63. A chiral Eu3+-thienoyltrifluoroacetone complex on an avidin tetramer: luminescence and CD studies on the supramolecular protein-metal chelate complex

    Taki, M. and Murakami, H. and Sisido, M.

    Chem. Commun.   ( 13 ) page: 1199-1200   2000

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  64. Site-directed incorporation of fluorescent nonnatural amino acids into streptavidin for highly sensitive detection of bioti

    Murakami, H. and Hohsaka, T. and Ashizuka, Y. and Hashimoto, K. and Sisido, M.

    Biomacromolecules   Vol. 1 ( 1 ) page: 118-125   2000

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    Language:English   Publishing type:Research paper (scientific journal)  

  65. Efficient incorporation of nonnatural amino acids with large aromatic groups into streptavidin in in vitro protein synthesizing systems

    Hohsaka, T. and Kajihara, D. and Ashizuka, Y. and Murakami, H. and Sisido, M.

    J. Am. Chem. Soc.   Vol. 121 ( 1 ) page: 34-40   1999

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  66. Incorporation of two different nonnatural amino acids independently into a single protein through extension of the genetic code

    Hohsaka, T. and Ashizuka, Y. and Sasaki, H. and Murakami, H. and Sisido, M.

    J. Am. Chem. Soc.   Vol. 121 ( 51 ) page: 12194-12195   1999

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  67. Site-directed incorporation of p-nitrophenylalanine into streptavidin and site-to-site photoinduced electron transfer from a pyrenyl group to a nitrophenyl group on the protein framework

    Murakami, H. and Hohsaka, T. and Ashizuka, Y. and Sisido, M.

    J. Am. Chem. Soc.   Vol. 120 ( 30 ) page: 7520-7529   1998

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  68. Incorporation of nonnatural amino acids into streptavidin through in vitro frame-shift suppression

    Hohsaka, T. and Ashizuka, Y. and Murakami, H. and Sisido, M.

    J. Am. Chem. Soc.   Vol. 118 ( 40 ) page: 9778-9779   1996

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Presentations 11

  1. 新型コロナウイルスに対する迅速な人工抗体創製 Invited

    近藤大志,岩谷靖雅,松岡和弘,藤野公茂,梅本駿,横幕能行,林剛介,村上裕

    構造生物学・化学・計算科学を融合させたウイルス・パンデミックに対する取り組み  2021.4.2 

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    Event date: 2021.4

    Language:Japanese  

  2. Ru触媒を用いたリンカーヒストンH1.2とHP1αの化学合成とその翻訳後修飾の機能解析

    加茂 直己、鯨井 智也、胡桃坂 仁志、村上 裕、林 剛介、岡本 晃充

    日本化学会 第101回春期年会  2021.3.22 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

  3. セレノシステインを用いたセレノエステル合成とペプチド連結反応への応用

    橋本 雅也、林 剛介、村上 裕

    日本化学会 第101回春期年会  2021.3.21 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

  4. High-speed selection of antibody-like proteins that capture and neutralize SARS-CoV-2 International conference

    2021.3.8 

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    Event date: 2021.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  5. 高速人工抗体創製法の開発とSARS-CoV-2中和抗体作製への応用 Invited

    村上裕

    生物化学的測定研究会 第25回学術シンポジウム  2020.11.6 

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  6. SARS-CoV-2を結合・中和する人工抗体の開発

    村上裕

    第14回バイオ関連化学シンポジウム  2020.9.8 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  7. SARS-CoV-2由来スパイクタンパク質に対する高親和性人工抗体の高速創製

    近藤 太志・岩谷 靖雅・松岡 和弘・藤野 公茂・梅本 駿・横幕 能行・林 剛介・村上 裕

    第14回バイオ関連化学シンポジウム  2020.9.7 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

  8. アミノ酸入れ替え遺伝暗号の開発

    藤野 公茂・戸崎 将弘・村上 裕

    第14回バイオ関連化学シンポジウム  2020.9.7 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

  9. 低分子リガンドに特異的に結合する人工抗体の創製

    都築 成晃・吉井 達之・近藤 大志・藤野 公茂・林 剛介・築地 真也・村上 裕

    第14回バイオ関連化学シンポジウム  2020.9.7 

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    Event date: 2020.9

    Language:Japanese   Presentation type:Poster presentation  

  10. TRAP提示法を用いた機能性ペプチド•人工抗体選択 Invited

    村上裕

    化学工学会第49回秋季大会 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  11. 翻訳合成系を用いた進化分子工学・合成生物学 Invited

    村上裕

    ChemBio講演会2017-1 

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    Event date: 2017.1

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

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Research Project for Joint Research, Competitive Funding, etc. 1

  1. 人工RNA触媒を用いた革新的な創薬技術の開発

    2005.4 - 2008.3

    分野横断的公募事業 若手研究グラント 3年型 

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    Grant type:Competitive

KAKENHI (Grants-in-Aid for Scientific Research) 6

  1. 抗体医薬を真に置き換える小タンパク質医薬の開発

    Grant number:21H02061  2021.4 - 2025.3

    科学研究費助成事業  基盤研究(B)

    森 健, 村上 裕

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    Authorship:Coinvestigator(s) 

    本研究では、二つの目的を達成する小タンパク質医薬を創製する。一つは抗体医薬の問題である高い薬価を克服する安価なタンパク質医薬でありながら、抗体と同等の細胞傷害活性を持つFc-ARMである。もう一つは、抗がん薬に対するがんの耐性獲得の問題を克服する小タンパク質医薬pH-ARMである。Fc-ARMの概念は申請者が報告してきたものであるが、本研究では、Fc親和性の高い小タンパク質を大規模な無細胞系RNAライブラリーよりスクリーニングして見いだし、用いることで抗体医薬に匹敵する性能を発揮すると期待される。また、pH-ARMは新しい概念であり、耐性がんの克服は、未解決の課題であり、本法は原理的にその問題を解決しうるものである。

  2. 高速人工抗体創製法 の開発とタンパク質 1分子単位定量への 応用

    2015.4 - 2019.3

    科学研究費補助金  基盤研究(A)

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    Authorship:Principal investigator 

  3. 新遺伝暗号の創成

    2015.4 - 2016.3

    科学研究費補助金 

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    Authorship:Principal investigator 

  4. JSPS 特殊ペプチド増幅法の開発と創薬への応

    2010.4 - 2014.3

    科学研究費補助金  最先端・次世代研究開発支援プログラ

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    Authorship:Principal investigator 

  5. ポリペプチド翻訳合成:D体アミノ酸とベータアミノ酸への新展開

    2008.4 - 2011.3

    科学研究費補助金  若手研究(A)

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    Authorship:Principal investigator 

  6. 新規アミノアシルtRNA合成リボザイムの創製

    2005.4 - 2007.3

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

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Industrial property rights 7

  1. ポリペプチドを製造するための新遺伝暗号と翻訳系、及びポリペプチドの製造方法

    村上 裕、石沢尭大

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    Application no:2015-19173  Date applied:2015.9

    Country of applicant:Domestic  

  2. 荷電性非タンパク質性アミノ酸含有ペプチドの製造方法

    村上 裕、川上 隆史、パトリック・リード、佐々木 亨

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    Applicant:国立大学法人東京大学、ペプチドリーム株式会社

    Application no:特願2013-162440  Date applied:2014.8

    Country of applicant:Domestic  

  3. ペプチドライブラリの製造方法、ペプチドライブラリ、及びスクリーニング方法

    村上 裕、川上隆史、石沢尭大、パトリック・リード、佐々木亨

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    Applicant:国立大学法人東京大学、ペプチドリーム株式会社

    Application no:特願2013-100661  Date applied:2013.5

    Country of applicant:Domestic  

  4. 血管内皮細胞増殖因子受容体阻害ペプチド

    村上 裕、川上隆史、石沢尭大、菅裕明、パトリック・リード

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    Applicant:国立大学法人東京大学、ペプチドリーム株式会社

    Application no:特願2012-193453  Date applied:2012.9

    Country of applicant:Domestic  

  5. 新規人工翻訳合成系

    菅 裕明、村上 裕、後藤 佑樹

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    Applicant:国立大学法人東京大学、ペプチドリーム株式会社

    Application no:特願2010-270958  Date applied:2011.8

    Country of applicant:Domestic  

  6. 多目的アシル化触媒とその用途

    菅裕明、村上裕

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    Applicant:国立大学法人東京大学

    Application no:特許第5119444号  Date applied:2005.12

    Country of applicant:Domestic  

  7. RIBOZYMES WITH BROAD tRNA AMINOACYLATION ACTIVITY

    SUGA, Hiroaki, MURAKAMI, Hiroshi, SAITO, Hirohide

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    Applicant:THE RESEARCH FOUNDATION OF STATE UNIVERSITY OF NEW YORK

    Application no:米国US Patent 7,622,248 B2  Date applied:2003.2

    Country of applicant:Domestic  

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Media Coverage 3

  1. 新型コロナに対する人工抗体 TV or radio program

    NHK、CBC放送  2020.9

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    Author:Other 

  2. 新型コロナ捕まえる「人工抗体」 短期間で作る手法開発 Newspaper, magazine

    読売新聞、中日新聞、朝日新聞、共同通信、等その他12社  2020.9

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    Author:Other 

  3. Vol.7 かつてない速さで抗体をつくる〜新型コロナウイルスとその先へ〜 Internet

    名古屋大学産学連携  2020.11