Updated on 2021/05/22

写真a

 
OHKA Fumiharu
 
Organization
Graduate School of Medicine Program in Integrated Medicine Clinical Neurosciences Lecturer
Graduate School
Graduate School of Medicine
Undergraduate School
School of Medicine Department of Medicine
Title
Lecturer

Degree 1

  1. 博士(医学) ( 2013.3   名古屋大学 ) 

Research History 5

  1. 名古屋大学大学院医学系研究科   脳神経外科   助教

    2016.8

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    Country:Japan

  2. 名古屋大学医学部附属病院   脳神経外科   病院助教

    2015.4 - 2016.7

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    Country:Japan

  3. 日本学術振興会特別研究員(PD)

    2014.4 - 2015.3

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    Country:Japan

  4. 愛知県がんセンター研究所   リサーチレジデント

    2012.4 - 2014.3

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    Country:Japan

  5. 名古屋大学医学部附属病院   医員

    2009.4 - 2010.3

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    Country:Japan

Education 2

  1. Nagoya University   Graduate School, Division of Medical Sciences

    - 2013.3

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    Country: Japan

  2. Nagoya University   Faculty of Medicine

    - 2004.3

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    Country: Japan

Professional Memberships 5

  1. 日本脳神経外科学会

  2. 日本癌学会

  3. 日本脳腫瘍学会

  4. 日本脳腫瘍病理学会

  5. Society of Neuro-Oncology

Awards 4

  1. 第26回日本脳神経外科学会奨励賞

    2020.10   日本脳神経外科学会  

    大岡史治

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

  2. Young Investigator Clinical Research Abstract Award

    2016.11   Society of Neuro-Oncology  

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    Award type:Award from international society, conference, symposium, etc.  Country:United States

  3. 日本脳腫瘍病理学会・学会賞

    2018.9   日本脳腫瘍病理学会  

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    Award type:International academic award (Japan or overseas)  Country:Japan

  4. 名古屋大学医師会研究奨励賞

    2012   名古屋大学医師会  

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    Country:Japan

 

Papers 47

  1. Urinary MicroRNA-Based Diagnostic Model for Central Nervous System Tumors Using Nanowire Scaffolds.

    Kitano Y, Aoki K, Ohka F, Yamazaki S, Motomura K, Tanahashi K, Hirano M, Naganawa T, Iida M, Shiraki Y, Nishikawa T, Shimizu H, Yamaguchi J, Maeda S, Suzuki H, Wakabayashi T, Baba Y, Yasui T, Natsume A

    ACS applied materials & interfaces   Vol. 13 ( 15 ) page: 17316 - 17329   2021.4

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  2. Expression of programmed cell death ligand-1 by immune cells in the microenvironment is a favorable prognostic factor for primary diffuse large B-cell lymphoma of the central nervous system.

    Tsuyuki Y, Ishikawa E, Kohno K, Shimada K, Ohka F, Suzuki Y, Mabuchi S, Satou A, Takahara T, Kato S, Miyagi S, Ozawa H, Kawano T, Takagi Y, Hiraga J, Wakabayashi T, Nakamura S

    Neuropathology : official journal of the Japanese Society of Neuropathology   Vol. 41 ( 2 ) page: 99 - 108   2021.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/neup.12705

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  3. Trautmann-focused mastoidectomy for a simple, safe presigmoid approach: technical note.

    Tanahashi K, Uda K, Araki Y, Takeuchi K, Choo J, Chalise L, Motomura K, Ohka F, Wakabayashi T, Natsume A

    Journal of neurosurgery   Vol. 134 ( 3 ) page: 843 - 847   2021.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3171/2020.1.JNS193179

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  4. Neurod4 converts endogenous neural stem cells to neurons with synaptic formation after spinal cord injury

    Fukuoka Toshiki, Kato Akira, Hirano Masaki, Ohka Fumiharu, Aoki Kosuke, Awaya Takayuki, Adilijiang Alimu, Sachi Maeda, Tanahashi Kuniaki, Yamaguchi Junya, Motomura Kazuya, Shimizu Hiroyuki, Nagashima Yoshitaka, Ando Ryo, Wakabayashi Toshihiko, Lee-Liu Dasfne, Larrain Juan, Nishimura Yusuke, Natsume Atsushi

    ISCIENCE   Vol. 24 ( 2 ) page: 102074   2021.2

  5. Intraoperative seizure outcome of levetiracetam combined with perampanel therapy in patients with glioma undergoing awake brain surgery.

    Motomura K, Chalise L, Shimizu H, Yamaguchi J, Nishikawa T, Ohka F, Aoki K, Tanahashi K, Hirano M, Wakabayashi T, Natsume A

    Journal of neurosurgery     page: 1 - 10   2021.1

  6. INFANTILE HEMISPHERIC BRAIN TUMOR WITH A GOPC-ROS1 FUSION GENE: A CASE REPORT

    Taniguchi Rieko, Natsume Atsushi, Kawashima Nozomu, Tanioka Shinji, Muramatsu Hideki, Hamada Motoharu, Ichikawa Daisuke, Imaya Masayuki, Narita Kotaro, Kurimoto Michihiro, Shiba Yoshiki, Aoki Kosuke, Ohka Fumiharu, Hirato Junko, Wakabayashi Toshihiko, Takahashi Yoshiyuki

    NEURO-ONCOLOGY   Vol. 22   page: 377 - 377   2020.12

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  7. Solitary fibrous tumor/hemangiopericytoma treated with temozolomide plus bevacizumab: a report of four cases and literature review

    Maeda Osamu, Ohka Fumiharu, Maesawa Satoshi, Matsuoka Ayumu, Shimokata Tomoya, Mitsuma Ayako, Urakawa Hiroshi, Nakamura Shota, Shimoyamas Yoshic, Nakaguro Masato S., Wakabayashi Toshihiko, Ando Yuichi

    NAGOYA JOURNAL OF MEDICAL SCIENCE   Vol. 82 ( 4 ) page: 631 - 644   2020.11

  8. Long-term survival in patients with primary intracranial germ cell tumors treated with surgery, platinum-based chemotherapy, and radiotherapy: a single-institution study.

    Shimizu H, Motomura K, Ohka F, Aoki K, Tanahashi K, Hirano M, Chalise L, Nishikawa T, Yamaguchi J, Yoshida J, Natsume A, Wakabayashi T

    Journal of neurosurgery     page: 1 - 9   2020.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3171/2020.6.JNS20638

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  9. Neurocognitive and functional outcomes in patients with diffuse frontal lower-grade gliomas undergoing intraoperative awake brain mapping.

    Motomura K, Chalise L, Ohka F, Aoki K, Tanahashi K, Hirano M, Nishikawa T, Yamaguchi J, Shimizu H, Wakabayashi T, Natsume A

    Journal of neurosurgery   Vol. 132 ( 6 ) page: 1683 - 1691   2020.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3171/2019.3.JNS19211

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  10. Steroid-responsive recurrent tumefactive demyelination with multiple petechial hemorrhages along non-displaced medullary veins.

    Tsuboi T, Harada Y, Suzuki M, Ando T, Atsuta N, Ohka F, Takeuchi K, Taoka T, Ohba S, Nakaguro M, Abe M, Nakashima I, Yoshida M, Katsuno M

    Clinical neurology and neurosurgery   Vol. 193   page: 105764   2020.6

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.clineuro.2020.105764

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  11. Genetic analysis in patients with newly diagnosed glioblastomas treated with interferon-beta plus temozolomide in comparison with temozolomide alone

    Natsume Atsushi, Aoki Kosuke, Ohka Fumiharu, Maeda Sachi, Hirano Masaki, Adilijiang Alimu, Motomura Kazuya, Sumi Minako, Nishikawa Ryo, Narita Yoshitaka, Muragaki Yoshihiro, Maruyama Takashi, Ito Tamio, Beppu Takaaki, Nakamura Hideo, Kayama Takamasa, Sato Shinya, Nagane Motoo, Mishima Kazuhiko, Nakasu Yoko, Kurisu Kaoru, Yamasaki Fumiyuki, Sugiyama Kazuhiko, Onishi Takanori, Iwadate Yasuo, Terasaki Mizuhiko, Kobayashi Hiroyuki, Matsumura Akira, Ishikawa Eiichi, Sasaki Hikaru, Mukasa Akitake, Matsuo Takayuki, Hirano Hirofumi, Kumabe Toshihiro, Shinoura Nobusada, Hashimoto Naoya, Aoki Tomokazu, Asai Akio, Abe Tatsuya, Yoshino Atsuo, Arakawa Yoshiki, Asano Kenichiro, Yoshimoto Koji, Shibui Soichiro, Okuno Yusuke, Wakabayashi Toshihiko

    JOURNAL OF NEURO-ONCOLOGY   Vol. 148 ( 1 ) page: 17 - 27   2020.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s11060-020-03505-9

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  12. H3F3A mutant allele specific imbalance in an aggressive subtype of diffuse midline glioma, H3 K27M-mutant

    Maeda Sachi, Ohka Fumiharu, Okuno Yusuke, Aoki Kosuke, Motomura Kazuya, Takeuchi Kazuhito, Kusakari Hironao, Yanagisawa Nobuyuki, Sato Shinya, Yamaguchi Junya, Tanahashi Kuniaki, Hirano Masaki, Kato Akira, Shimizu Hiroyuki, Kitano Yotaro, Yamazaki Shintaro, Yamashita Shinji, Takeshima Hideo, Shinjo Keiko, Kondo Yutaka, Wakabayashi Toshihiko, Natsume Atsushi

    ACTA NEUROPATHOLOGICA COMMUNICATIONS   Vol. 8 ( 1 ) page: 8   2020.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1186/s40478-020-0882-4

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  13. Multiple metastases to the bone and bone marrow from a 1p/19q-codeleted and <i>IDH2</i>-mutant anaplastic oligodendroglioma: a case report and literature review.

    Shimizu H, Motomura K, Ohka F, Aoki K, Tanahashi K, Hirano M, Chalise L, Nishikawa T, Yamaguchi J, Wakabayashi T, Natsume A

    Neuro-oncology advances   Vol. 2 ( 1 ) page: vdaa101   2020.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/noajnl/vdaa101

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  14. MACHINE LEARNING TO DETECT GLIOMAS IN URINE-BASED LIQUID BIOPSY

    Kitano Yotaro, Aoki Kosuke, Yasui Takao, Motomura Kazuya, Ohka Fumiharu, Tanahashi Kuniaki, Hirano Masaki, Nishikawa Tomohide, Shimizu Hiroyuki, Yamaguchi Junya, Maeda Sachi, Yamazaki Shintaro, Wakabayashi Toshihiko, Natsume Atsushi

    NEURO-ONCOLOGY   Vol. 21   page: 151 - 151   2019.11

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  15. Pathogenic Epigenetic Consequences of Genetic Alterations in IDH-wild-type Diffuse Astrocytic Gliomas.

    Cancer research   Vol. 79 ( 19 ) page: 4814 - 4827   2019.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1158/0008-5472.CAN-19-1272

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  16. Spontaneous Tumor Regression of Intracranial Solitary Fibrous Tumor Originating From the Medulla Oblongata: A Case Report and Literature Review.

    Yamaguchi J, Motomura K, Ohka F, Aoki K, Tanahashi K, Hirano M, Nishikawa T, Shimizu H, Wakabayashi T, Natsume A

    World neurosurgery   Vol. 130   page: 400 - 404   2019.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.wneu.2019.07.052

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  17. Next Generation Sequencing-Based Transcriptome Predicts Bevacizumab Efficacy in Combination with Temozolomide in Glioblastoma.

    Adilijiang A, Hirano M, Okuno Y, Aoki K, Ohka F, Maeda S, Tanahashi K, Motomura K, Shimizu H, Yamaguchi J, Wakabayashi T, Natsume A

    Molecules (Basel, Switzerland)   Vol. 24 ( 17 )   2019.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/molecules24173046

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  18. Aberrant Active cis-Regulatory Elements Associated with Downregulation of RET Finger Protein Overcome Chemoresistance in Glioblastoma

    Ranjit Melissa, Hirano Masaki, Aoki Kosuke, Okuno Yusuke, Ohka Fumiharu, Yamamichi Akane, Kato Akira, Maeda Sachi, Motomura Kazuya, Matsuo Keitaro, Enomoto Atsushi, Ino Yasushi, Todo Tomoki, Takahashi Masahide, Wakabayashi Toshihiko, Kato Takuya, Natsume Atsushi

    CELL REPORTS   Vol. 26 ( 9 ) page: 2274-+   2019.2

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    DOI: 10.1016/j.celrep.2019.01.109

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  19. Aberrant active-enhancers associated with downregulation of HDAC1-RFP complex overcome chemoresistance in glioblastoma

    Hirano Masaki, Melissa Ranjit, Ohka Fumiharu, Aoki Kosuke, Yamamichi Akane, Kato Takuya, Matsuo Keitaro, Enomoto Atsushi, Takahashi Masahide, Wakabayashi Toshihiko, Natsume Atsushi

    CANCER SCIENCE   Vol. 109   page: 207-207   2018.12

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  20. Development of anticancer agents targeting long non-coding RNA

    Katsushima Keisuke, Natsume Atsushi, Ohka Fumiharu, Shinjo Keiko, Shibata Tatsuhiro, Miyata Kanjiro, Kataoka Kazunori, Kondo Yutaka

    CANCER SCIENCE   Vol. 109   page: 352-352   2018.12

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  21. ABERRANT ACTIVE-ENHANCERS ASSOCIATED WITH DOWNREGULATION OF HDAC1-RET FINGER PROTEIN COMPLEX OVERCOME CHEMORESISTANCE IN GLIOBLASTOMA

    Hirano Masaki, Ranjit Melissa, Ohka Fumiharu, Aoki Kosuke, Yamamichi Akane, Kato Takuya, Matsuo Keitaro, Enomoto Atsushi, Takahashi Masahide, Wakabayashi Toshihiko, Natsume Atsushi

    NEURO-ONCOLOGY   Vol. 20   page: 111-111   2018.11

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  22. Validation of a novel molecular RPA classification in glioblastoma (GBM-molRPA) treated with chemoradiation: A multi-institutional collaborative study

    Wee Chan Woo, Kim Il Han, Park Chul-Kee, Kim Jin Wook, Dho Yun-Sik, Ohka Fumiharu, Aoki Kosuke, Motomura Kazuya, Natsume Atsushi, Kim Nalee, Suh Chang-Ok, Chang Jong Hee, Kim Se Hoon, Cho Won Kyung, Lim Do Hoon, Nam Do-Hyun, Choi Jung Won, Kim In Ah, Kim Chae-Yong, Oh Young-Taek, Cho Oyeon, Chung Woong-Ki, Kim Sung-Hwan, Kim Eunji

    RADIOTHERAPY AND ONCOLOGY   Vol. 129 ( 2 ) page: 347 - 351   2018.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.radonc.2018.09.001

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  23. Supratotal Resection of Diffuse Frontal Lower Grade Gliomas with Awake Brain Mapping, Preserving Motor, Language, and Neurocognitive Functions

    Motomura Kazuya, Chalise Lushun, Ohka Fumiharu, Aoki Kosuke, Tanahashi Kuniaki, Hirano Masaki, Nishikawa Tomohide, Wakabayashi Toshihiko, Natsume Atsushi

    WORLD NEUROSURGERY   Vol. 119   page: 30-39   2018.11

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    DOI: 10.1016/j.wneu.2018.07.193

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  24. Characterization of lntraoperative Motor Evoked Potential Monitoring for Surgery of the Pediatric Population with Brain Tumors

    Motomura Kazuya, Sumita Kayo, Chalise Lushun, Nishikawa Tomohide, Tanahashi Kuniaki, Ohka Fumiharu, Aoki Kosuke, Hirano Masaki, Nakamura Tomohiko, Matsushita Tadashi, Wakabayashi Toshihiko, Natsume Atsushi

    WORLD NEUROSURGERY   Vol. 119   page: E1052-E1059   2018.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.wneu.2018.08.039

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  25. Neuroendoscopic Cylinder Surgery and 5-Aminolevulinic Acid Photodynamic Diagnosis of Deep-Seated Intracranial Lesions

    Choo Jungsu, Takeuchi Kazuhito, Nagata Yuichi, Ohka Fumiharu, Kishida Yugo, Watanabe Tadashi, Satoh Yusuke, Nagatani Tetsuya, Kato Kyozo, Wakabayashi Toshihiko, Natsume Atsushi

    WORLD NEUROSURGERY   Vol. 116   page: E35-E41   2018.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.wneu.2018.03.112

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  26. Immunohistochemical ATRX expression is not a surrogate for 1p19q codeletion

    Yamamichi Akane, Ohka Fumiharu, Aoki Kosuke, Suzuki Hiromichi, Kato Akira, Hirano Masaki, Motomura Kazuya, Tanahashi Kuniaki, Chalise Lushun, Maeda Sachi, Wakabayashi Toshihiko, Kato Yukinari, Natsume Atsushi

    BRAIN TUMOR PATHOLOGY   Vol. 35 ( 2 ) page: 106-113   2018.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10014-018-0312-5

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  27. A novel high-sensitivity assay to detect a small fraction of mutant IDH1 using droplet digital PCR.

    Hirano M, Ohka F, Maeda S, Chalise L, Yamamichi A, Aoki K, Kato A, Tanahashi K, Motomura K, Nishimura Y, Hara M, Shinjo K, Kondo Y, Wakabayashi T, Natsume A

    Brain tumor pathology   Vol. 35 ( 2 ) page: 97 - 105   2018.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10014-018-0310-7

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  28. Prognostic relevance of genetic alterations in diffuse lower-grade gliomas

    Aoki Kosuke, Nakamura Hideo, Suzuki Hiromichi, Matsuo Keitaro, Kataoka Keisuke, Shimamura Teppei, Motomura Kazuya, Ohka Fumiharu, Shiina Satoshi, Yamamoto Takashi, Nagata Yasunobu, Yoshizato Tetsuichi, Mizoguchi Masahiro, Abe Tatsuya, Momii Yasutomo, Muragaki Yoshihiro, Watanabe Reiko, Ito Ichiro, Sanada Masashi, Yajima Hironori, Morita Naoya, Takeuchi Ichiro, Miyano Satoru, Wakabayashi Toshihiko, Ogawa Seishi, Natsume Atsushi

    NEURO-ONCOLOGY   Vol. 20 ( 1 ) page: 66-77   2018.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/neuonc/nox132

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  29. Aberrant super-enhancers associated with downregulation of RET finger protein overcomes chemoresistance in glioblastoma

    Hirano Masaki, Ranjit Melissa, Yamamichi Akane, Aoki Kosuke, Ohka Fumiharu, Kato Takuya, Enomoto Atsushi, Takahashi Masahide, Wakabayashi Toshihiko, Natsume Atsushi

    CANCER SCIENCE   Vol. 109   page: 461-461   2018.1

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  30. Targeting Dysregulation of EZH2-H3K27me3 as an Effective Treatment for IDH-wildtype Lower Grade Glioma

    Ohka Fumiharu, Natsume Atsushi, Suzuki Hiromichi, Aoki Kosuke, Deguchi Shoichi, Katsushima Keisuke, Shinjo Keiko, Wakabayashi Toshihiko, Kondo Yutaka

    CANCER SCIENCE   Vol. 109   page: 463-463   2018.1

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  31. Does ATRX immunohistochemistry deserve a surrogate of 1p/19q codel in grade II, III gliomas?

    Yamamichi Akane, Ohka Fumiharu, Aoki Kosuke, Suzuki Hiromichi, Hirano Masaki, Motomura Kazuya, Wakabayashi Toshihiko, Kato Yukinari, Natsume Atsushi

    CANCER SCIENCE   Vol. 109   page: 926-926   2018.1

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  32. Development of anticancer agents targeting long non-coding RNA TUG1

    Katsushima Keisuke, Natsume Atsushi, Ohka Fumiharu, Deguchi Shoichi, Shinjo Keiko, Shibata Tatsuhiro, Miyata Kanjiro, Kataoka Kazunori, Kondo Yutaka

    CANCER SCIENCE   Vol. 109   page: 398-398   2018.1

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  33. Significance of perivascular tumour cells defined by CD109 expression in progression of glioma

    Shiraki Yukihiro, Mii Shinji, Enomoto Atsushi, Momota Hiroyuki, Han Yi-Peng, Kato Takuya, Ushida Kaori, Kato Akira, Asai Naoya, Murakumo Yoshiki, Aoki Kosuke, Suzuki Hiromichi, Ohka Fumiharu, Wakabayashi Toshihiko, Todo Tomoki, Ogawa Seishi, Natsume Atsushi, Takahashi Masahide

    JOURNAL OF PATHOLOGY   Vol. 243 ( 4 ) page: 468-480   2017.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/path.4981

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  34. Organotypic brain explant culture as a drug evaluation system for malignant brain tumors

    Minami Noriaki, Maeda Yusuke, Shibao Shunsuke, Arima Yoshimi, Ohka Fumiharu, Kondo Yutaka, Maruyama Koji, Kusuhara Masatoshi, Sasayama Takashi, Kohmura Eiji, Saya Hideyuki, Sampetrean Oltea

    CANCER MEDICINE   Vol. 6 ( 11 ) page: 2635-2645   2017.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cam4.1174

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  35. Negative impact of leukoaraiosis on the incidence of brain metastases in patients with lung cancer

    Hayashi Nakamasa, Mitsuya Koichi, Nakasu Yoko, Naito Tateaki, Ohka Fumiharu, Takahashi Toshiaki

    JOURNAL OF NEURO-ONCOLOGY   Vol. 135 ( 2 ) page: 299-306   2017.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s11060-017-2574-x

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  36. Remote ischemic preconditioning protects human neural stem cells from oxidative stress

    Motomura Ayako, Shimizu Mikiko, Kato Akira, Motomura Kazuya, Yamamichi Akane, Koyama Hiroko, Ohka Fumiharu, Nishikawa Tomohide, Nishimura Yusuke, Hara Masahito, Fukuda Tetsuya, Bando Yasuhiko, Nishimura Toshihide, Wakabayashi Toshihiko, Natsume Atsushi

    APOPTOSIS   Vol. 22 ( 11 ) page: 1353-1361   2017.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10495-017-1425-8

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  37. ABERRANT SUPER-ENHANCERS ASSOCIATED WITH DOWNREGULATION OF RET FINGER PROTEIN OVERCOMES CHEMORESISTANCE IN GLIOBLASTOMA

    Hirano Masaki, Ranjit Melissa, Yamamichi Akane, Aoki Kosuke, Ohka Fumiharu, Kato Takuya, Enomoto Atsushi, Takahashi Masahide, Wakabayashi Toshihiko, Natsume Atsushi

    NEURO-ONCOLOGY   Vol. 19   page: 103-103   2017.11

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  38. POLYION COMPLEX-MEDIATED INTRAVENOUS DELIVERY OF ANTISENSE OLIGONUCLEOTIDES AGAINST THE NOTCH-REGULATED NON-CODING RNA TUG1 IN GLIOMA

    Natsume Atsushi, Katsushima Keisuke, Ohka Fumiharu, Shinjo Keiko, Miyata Kanjiro, Kataoka Kazunori, Kondo Yutaka

    NEURO-ONCOLOGY   Vol. 19   page: 265-265   2017.11

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  39. IS ATRX IMMUNOCHEMISTRY USEFUL AS A SUBSTITUTE FOR ANALYSIS OF 1p/19q CODELETION IN GRADES II, III GLIOMAS?

    Yamamichi Akane, Ohka Fumiharu, Aoki Kosuke, Suzuki Hiromichi, Hirano Masaki, Motomura Kazuya, Wakabayashi Toshihiko, Kato Yukinari, Natsume Atsushi

    NEURO-ONCOLOGY   Vol. 19   page: 180-180   2017.11

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  40. Comparing the Efficacy of DeVIC Therapy and High-dose Methotrexate Monotherapy with Whole-brain Radiation Therapy for Newly-diagnosed Primary Central Nervous System Lymphoma: A Single Institution Study

    Chalise Lushun, Motomura Kazuya, Ohka Fumiharu, Hirano Masaki, Hara Masahito, Nishimura Yusuke, Natsume Atsushi, Wakabayashi Toshihiko

    ANTICANCER RESEARCH   Vol. 37 ( 9 ) page: 5215-5223   2017.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.21873/anticanres.11945

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  41. Oncogenic effects of evolutionarily conserved noncoding RNA ECONEXIN on gliomagenesis

    Deguchi S., Katsushima K., Hatanaka A., Shinjo K., Ohka F., Wakabayashi T., Zong H., Natsume A., Kondo Y.

    ONCOGENE   Vol. 36 ( 32 ) page: 4629-4640   2017.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/onc.2017.88

    Web of Science

  42. PHASE I TRIAL OF TEMOZOLOMIDE PLUS BEVACIZUMAB FOR NEWLY DIAGNOSED HIGH-GRADE GLIOMAS IN THE ELDERLY: INTERIM REPORT

    Kono M., Arakawa Y., Mineharu Y., Ohka F., Kinoshita M., Nakae S., Miyashita K., Iuchi T., Hirose Y., Natsume A., Nakada M., Sasaki H.

    NEURO-ONCOLOGY   Vol. 19   page: 81-81   2017.5

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    Web of Science

  43. Significance of low mTORC1 activity in defining the characteristics of brain tumor stem cells

    Han Yi-Peng, Enomoto Atsushi, Shiraki Yukihiro, Wang Shen-Qi, Wang Xiaoze, Toyokuni Shinya, Asai Naoya, Ushida Kaori, Ara Hosne, Ohka Fumiharu, Wakabayashi Toshihiko, Ma Jie, Natsume Atsushi, Takahashi Masahide

    NEURO-ONCOLOGY   Vol. 19 ( 5 ) page: 636-647   2017.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/neuonc/now237

    Web of Science

    PubMed

  44. A novel all-in-one intraoperative genotyping system for IDH1-mutant glioma

    Ohka Fumiharu, Yamamichi Akane, Kurimoto Michihiro, Motomura Kazuya, Tanahashi Kuniaki, Suzuki Hiromichi, Aoki Kosuke, Deguchi Shoichi, Chalise Lushun, Hirano Masaki, Kato Akira, Nishimura Yusuke, Hara Masahito, Kato Yukinari, Wakabayashi Toshihiko, Natsume Atsushi

    BRAIN TUMOR PATHOLOGY   Vol. 34 ( 2 ) page: 91-97   2017.4

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    DOI: 10.1007/s10014-017-0281-0

    Web of Science

    PubMed

  45. Quantitative metabolome analysis profiles activation of glutaminolysis in glioma with IDH1 mutation

    Fumiharu Ohka, Maki Ito, Melissa Ranjit, Takeshi Senga, Ayako Motomura, Kazuya Motomura, Kaori Saito, Keiko Kato, Yukinari Kato, Toshihiko Wakabayashi, Tomoyoshi Soga, Atsushi Natsume

    Tumor Biology     2014.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s13277-014-1784-5

  46. Current trends in targeted therapies for glioblastoma multiforme

    Fumiharu Ohka, Atsushi Natsume, Toshihiko Wakabayashi

    Neurology Research International     2012.3

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    Language:English  

    DOI: 10.1155/2012/878425

  47. The Global DNA Methylation Surrogate LINE-1 Methylation Is Correlated with MGMT Promoter Methylation and Is a Better Prognostic Factor for Glioma Reviewed

    Fumiharu Ohka, Atsushi Natsume, Kazuya Motomura, Yugo Kishida, Yutaka Kondo, Tatsuya Abe, Yoko Nakasu, Hiroki Namba, Kenji Wakai, Takashi Fukui, Hiroyuki Momota, Kenichiro Iwami, Sayano Kinjo, Maki Ito, Masazumi Fujii, Toshihiko Wakabayashi

    PLoS ONE     2011.8

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    DOI: 10.1371/journal.pone.0023332

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Books 1

  1. Epegenetic Cancer Therapy, CLINICAL SIGNIFICANCE OF EPIGENETIC ALTERATIONS IN GLIOBLASTOMA

    Ohka F, Natsume A, Kondo Y( Role: Joint author)

    ACADEMIC PRESS  2015 

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    Language:English

Presentations 2

  1. Role of Dysregulation of EZH2-H3K27me3 in Gliomagenesis

    Ohka F, Natsume A, Deguchi S, Katsushima K, Shinjo K, Wakabayashi T, Kondo Y

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    Event date: 2015.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  2. 次世代型術中decision-makingのための術中分子診断法の確立

    大岡史治、栗本路弘、山道茜、鈴木啓道、青木恒介、近藤五郎、本村和也、若林俊彦、夏目敦至

    第20回日本脳腫瘍の外科学会 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

Research Project for Joint Research, Competitive Funding, etc. 5

  1. 悪性脳腫瘍のオルガノイドモデルを用いた新規エピゲノム治療の探索

    2020.9

    武田科学振興財団医学系研究助成 

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    Grant type:Competitive

  2. G34R/V変異小児悪性神経膠腫の腫瘍形成メカニズムの解明

    2018 - 2019

    上原記念生命科学財団リサーチフェローシップ 

    大岡史治

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    Grant type:Competitive

  3. 小児毛様細胞性星細胞腫(Pilocytic astrocytoma)のLiquid biopsyを目指した、BRAF遺伝子異常の迅速高感度解析法の確立

    2017.4 - 2018.3

    日比野基金医学研究助成 

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    Grant type:Competitive

  4. 小児脳幹グリオーマのLiquid biopsyを目指したH3F3A遺伝子変異の高感度診断法の確立

    2017.4 - 2018.3

    日本脳神経財団研究助成 

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    Grant type:Competitive

  5. 高感度迅速遺伝子解析システムを用いた、liquid biopsyによる小児、若年成人脳腫瘍の新規低侵襲診断法の開発

    2017.4 - 2018.3

    共済団医学奨励研究助成 

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    Grant type:Competitive

KAKENHI (Grants-in-Aid for Scientific Research) 9

  1. The evolutional trajection and precision-medicine to gliomas

    Grant number:20H03789  2020.4 - 2023.3

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    Authorship:Coinvestigator(s) 

  2. Novel treatment strategy targeting neoantigen induced by epigenetic alterations in malignant glioma formation

    Grant number:19KK0228  2019.10 - 2023.3

    Fund for the Promotion of Joint International Research (Fostering Joint International Research (B))

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    Authorship:Coinvestigator(s) 

  3. スーパーエンハンサー形成が誘導する悪性脳腫瘍の異常クロマチンリモデリングの解明

    2019.4 - 2022.3

    科学研究費補助金  基盤研究(C)

    大岡 史治

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  4. スーパーエンハンサー形成が誘導する悪性脳腫瘍の異常クロマチンリモデリングの解明

    Grant number:19K09478  2019.4 - 2022.3

    大岡 史治

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    Authorship:Principal investigator 

    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

    近年、がんの新しいドライバーメカニズムとしてスーパーエンハンサー異常が提唱された。腫瘍形成に寄与する異常なスーパーエンハンサー機構の全容と、その出現タイミングを明確に解明することが悪性脳腫瘍の新規治療開発に重要と考えた。本研究では悪性脳腫瘍の形成過程において、経時的にスーパーエンハンサー領域と抑制型ヒストン修飾領域の変化を解析し、EZH2とスーパーエンハンサーのクロストークメカニズムを解明することを目的とする。本研究から得られた知見から予後不良疾患であるIDH野生型悪性脳腫瘍における、EZH2阻害剤とJQ1の併用新規治療法の開発につながることを期待して上記研究計画を進める予定である。
    本研究ではin vitro、in vivo実験を併用して、IDH 野生型悪性脳腫瘍の腫瘍形成過程におけるスーパーエンハンサー領域を経時的に解析し、治療標的としての有用性を評価することが目標である。当該年度はMADMマウスから回収したGFP陽性細胞を用いたChIP-Seq、RNA-Seq解析の条件検討を行った。MADMマウスの腫瘍形成前生後8日、60日のマウスと腫瘍形成後の生後120日のマウスからGFP陽性細胞を回収し、ホルマリン固定した後に抗H3K27ac抗体、抗H3K27me3抗体を用いたクロマチン沈降法(ChIP法)を行う手法を確立した。また同様にRNAの回収も行った。バイオアナライザーにて回収したRNAのクオリティー評価を行なった後にサンプル調整を行い、次世代シークエンサーにて解析しRPKM (Read Per Kilobase Million)を算出して発現量を解析した。これらの実験から得られたRPKMについてGene Ontology(GO)解析やGene Set Enrichment Analysis (GSEA)解析を行う準備を進めた。またin vitro実験ではEZH2阻害剤であるEPZ6438を細胞株に投与6日後に細胞を回収してウエスタンブロッティング法にてH3K27me3修飾が抑制されていることを確認した。同様にH3K27ac修飾の発現解析も行う予定である。EZH2阻害剤投与後にH3K27me3, H3K27acの修飾遺伝子領域をChIP-Seqにて解析し、 H3K27me3修飾がH3K27ac修飾に置き換わった部位を中心に解析し、それぞれの変化について解析をするための準備を進めている。MADM腫瘍細胞株にEPZ6438とスーパーエンハンサーの阻害作用を持つJQ1を投与するin vitro実験の準備も進めた。
    現在in vitro, in vivo実験ともに当初の予定に近いペースで進めることができている。今後は次世代シークエンスを用いた解析を進め、その解析系の確立も進める予定である。
    現在樹立しているin vitro, in vivo実験系を用いて、より詳細に解析をすすめる予定である。in vitro, in vivoともにJQ1投与実験を進める。またスーパーエンハンサー解析のためには次世代シークエンス解析の結果を詳細に解析する技術が必要になるので、その解析法の確立の準備も進めていく予定である。

  5. Xenopus由来因子のエピゲノム制御による神経系細胞の系譜転換と神経再生

    Grant number:19K09453  2019.4 - 2022.3

    西村 由介

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    これまでの大規模ゲノム解析での実績からアフリカツメガエル(Xenopus)での神経再生時に高発現し、内在性神経幹細胞を誘導する分子を選定する。新規に開発した神経幹細胞嗜好性のあるリンパ球性脈絡髄膜炎ウイルス(LCMV)のエンベロープと分裂細胞に感染し恒常的に発現するレトロウイルスのハイブリッドベクターを構築し、感染効率と発現効率上昇を両立させる技術を用いる。ヒストン脱アセチル化酵素を阻害することで興奮性運動ニューロンに誘導・分化させ、エピゲノム制御により細胞運命の系譜転換を起こし、軸索伸長とシナプス再構築と機能回復をマウス脊髄損傷モデルで検討する。
    2019年度は(i)アフリカツメガエル(Xenopus)での神経再生時に高発現し、内在性神経幹細胞を誘導する分子を選定すること(ii)ハイブリッドベクター(シュードウイルス)を構築し、脊髄損傷マウスの神経管に遺伝子導入することを予定した。それぞれの進捗状況について報告する。まずXenopusにおいて神経再生時にbHLH型転写因子であるNeurod4(E-boxにTcf3とへテロダイマーを形成することにより、下流の神経特異的遺伝子を活性化するという機能を持つ。) が高発現していることを特定した。ベクターに関してはリンパ球脈絡炎ウイルスをエンベロープとし、発現系はレトロウイルスであるというハイブリッドベクターを作成した。これにNeurod4、tauAcGFPを搭載して10週のマウスに髄腔内投与し、3日後に脊髄損傷させ、その6週間後の運動機能と構造について解析を行った。遺伝子の導入効率に関してはNestin陽性細胞の70%程度にGFPの発現が見られており、高率な導入が得られていることを確認した。6週間後の運動機能においてはBMSでの評価を行い、コントロール群と比較して遺伝子導入群で有意な改善が得られていることが確認できた。また、免疫染色の結果からNeurod4導入群ではNeuN陽性細胞、 ChAT陽性細胞の有意な増加が見られた。これらはqPCRでも確認を行い、構造的にも神経細胞の再生が得られたことが証明された。
    当初予定していた実験内容をほぼ計画通りに行うことができた。
    マウスモデル、遺伝子導入法の確立はできており、今後は遺伝子導入後の脊髄についてメカニズム解明のためにさらなる解析を行う予定である。

  6. Remodeling of altered epigenetic regulation via targeting epigenetic factor during glioma formation

    Grant number:16K20003  2016.4 - 2019.3

    Ohka Fumiharu

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    Authorship:Principal investigator 

    Grant amount:\3900000 ( Direct Cost: \3000000 、 Indirect Cost:\900000 )

    We established MADM mouse model which generates malignant glioma spontaneously. From an early stage of tumor formation, expression level of EZH2 was highly upregulated. Using glioma cell lines which are derived from MADM mice tumors, we found that EZH2 inhibitor (EPZ6438) inhibited cell growth of tumor cells. Also MRI imaging revealed that both oral administration of EPZ6438 and genetic depletion of EZH2 inhibited tumor growth in MADM mice significantly. We harvested tumor tissue of EZH2 knock-out MADM mice tumors, EZH2-intact MADM mice tumors and normal mice brains. Gene expression analysis with microarray revealed that expression levels of genes which are associated with neuronal differentiation are retained by knock out of EZH2 gene. These data indicated that EZH2 plays pivotal roles in glioma formation via dysregulation of histone modifications.

  7. エピゲノム修飾因子を標的とした悪性脳腫瘍の異常エピゲノムの修復

    2016.4 - 2018.3

    科学研究費補助金  若手研究(B)

    大岡 史治

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  8. 膠芽腫形成においてエピゲノム異常を誘導する新規分子の同定

    2014.4 - 2016.3

    科学研究費補助金  若手研究(B)

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  9. 悪性脳腫瘍におけるゲノムーエピゲノムのクロストークを標的とした新規治療の開発

    2014.4 - 2015.3

    科学研究費補助金 

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    Authorship:Principal investigator 

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