研究者詳細 - 清水　一憲

論文 - 清水　一憲

分割表示 131 件中 61 - 80 件目／全件表示 >>

Morphology-based non-invasive quantitative prediction of the differentiation status of neural stem cells.

Fujitani M, Huddin NS, Kawai S, Kanie K, Kiyota Y, Shimizu K, Honda H, Kato R

Journal of bioscience and bioengineering 124 巻 ( 3 ) 頁： 351-358 2017年9月

Using size-controlled multicellular spheroids of murine adenocarcinoma cells to efficiently establish pulmonary tumors in mice.

Nishikawa T, Tanaka Y, Kusamori K, Mizuno N, Mizukami Y, Ogino Y, Shimizu K, Konishi S, Takahashi Y, Takakura Y, Nishikawa M

Biotechnology journal 12 巻 ( 8 ) 2017年8月

Effective modification of cell death-inducing intracellular peptides by means of a photo-cleavable peptide array-based screening system.

Kozaki I, Shimizu K, Honda H

Journal of bioscience and bioengineering 124 巻 ( 2 ) 頁： 209-214 2017年8月

Alcohol-tolerant mutants of cyanobacterium Synechococcus elongatus PCC 7942 obtained by single-cell mutant screening system

Arai Sayuri, Hayashihara Kayoko, Kanamoto Yuki, Shimizu Kazunori, Hirokawa Yasutaka, Hanai Taizo, Murakami Akio, Honda Hiroyuki

BIOTECHNOLOGY AND BIOENGINEERING 114 巻 ( 8 ) 頁： 1771-1778 2017年8月

Three-Dimensional Culture Model of Skeletal Muscle Tissue with Atrophy Induced by Dexamethasone. Open Access

Shimizu K, Genma R, Gotou Y, Nagasaka S, Honda H

Bioengineering (Basel, Switzerland) 4 巻 ( 2 ) 頁： 56 2017年6月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Bioengineering

Drug screening systems for muscle atrophy based on the contractile force of cultured skeletal muscle tissues are required for the development of preventive or therapeutic drugs for atrophy. This study aims to develop a muscle atrophy model by inducing atrophy in normal muscle tissues constructed on microdevices capable of measuring the contractile force and to verify if this model is suitable for drug screening using the contractile force as an index. Tissue engineered skeletal muscles containing striated myotubes were prepared on the microdevices for the study. The addition of 100 µM dexamethasone (Dex), which is used as a muscle atrophy inducer, for 24 h reduced the contractile force significantly. An increase in the expression of Atrogin-1 and MuRF-1 in the tissues treated with Dex was established. A decrease in the number of striated myotubes was also observed in the tissues treated with Dex. Treatment with 8 ng/mL Insulin-like Growth Factor (IGF-I) for 24 h significantly increased the contractile force of the Dex-induced atrophic tissues. The same treatment, though, had no impact on the force of the normal tissues. Thus, it is envisaged that the atrophic skeletal muscle tissues induced by Dex can be used for drug screening against atrophy.

DOI： 10.3390/bioengineering4020056

Open Access

Scopus

PubMed

Image-based cell quality evaluation to detect irregularities under same culture process of human induced pluripotent stem cells.

Nagasaka R, Gotou Y, Yoshida K, Kanie K, Shimizu K, Honda H, Kato R

Journal of bioscience and bioengineering 123 巻 ( 5 ) 頁： 642-650 2017年5月

Optimization of albumin secretion and metabolic activity of cytochrome P450 1A1 of human hepatoblastoma HepG2 cells in multicellular spheroids by controlling spheroid size Open Access

Nishikawa T., Tanaka Y., Nishikawa M., Ogino Y., Kusamori K., Mizuno N., Mizukami Y., Shimizu K., Konishi S., Takahashi Y., Takakura Y.

Biological and Pharmaceutical Bulletin 40 巻 ( 3 ) 頁： 334 - 338 2017年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：Biological and Pharmaceutical Bulletin

Multicellular spheroids are useful as three-dimensional cell culture systems and for cell-based therapies. Their successful application requires an understanding of the consequences of spheroid size for cellular functions. In the present study, we prepared multicellular spheroids of different sizes using the human hepatoblastoma HepG2 cells, as hepatocytes are frequently used for in vitro drug screening and cell-based therapy. Precise polydimethylsiloxane-based microwells with widths of 360, 450, 560, and 770 μm were fabricated using a micromolding technique. Incubation of HepG2 cells in cell culture plates containing the microwells resulted in the formation of HepG2 spheroids with average diameters of 195, 320, 493, and 548 μm. The cell number per spheroid positively correlated with its diameter, and the viability of HepG2 cells was 94% or above for all samples. The smallest HepG2 spheroids showed the highest albumin secretion. On the other hand, the metabolic activity of 7-ethoxyresorufin, a fluorometric substrate for CYP1A1, increased with increasing spheroid size. These results indicate that controlling spheroid size is important when preparing HepG2 spheroids and that the size of HepG2 spheroids greatly influences the cellular function of HepG2 cells in the spheroids.

DOI： 10.1248/bpb.b16-00833

Open Access

Scopus

PubMed

Exploring high-affinity binding properties of octamer peptides by principal component analysis of tetramer peptides 査読有り

Kume, A., Kawai, S., Kato, R., Iwata, S., Shimizu, K., Honda, H.

Journal of Bioscience and Bioengineering 123 巻 ( 2 ) 頁： 230-238 2017年

Ex vivo culture of circulating tumor cells using magnetic force-based cell co-culture on a fibroblast feeder layer 査読有り

Yamamoto, S., Shimizu, K., Fei, J., Iwata, H., Okochi, M., Nakanishi, H., Honda, H.

Biotechnology Journal 頁： in press 2017年

Three-dimensional cultured skeletal muscle tissue atrophy model induced with dexamethasone 査読有り

Shimizu, K., Genma, R., Goto, Y., Nagasaka, S., Honda, H.

Bioengineering 4 巻 ( 2 ) 頁： 56 2017年

Morphology-based non-invasive quantitative prediction of the differentiation status of neural stem cells 査読有り

Fujitani, M., Huddin, N.S., Kawai, S., Kanie, K., Kiyota, Y., Shimizu, K., Honda, H., Kato, R.

Journal of Bioscience and Bioengineering 頁： in press 2017年

Characterization of transgene expression and pDNA distribution in the kidney by renal suction-mediated transfection method in mice 査読有り

Oyama, N., Fuchigami, Y., Fumoto, S., Sato, M., Hagimori M., Shimizu, K., Kawakami, S.

Drug Delivery 24 巻 ( 1 ) 頁： 906-917 2017年

Using size-controlled multicellular spheroids of murine adenocarcinoma cells to efficiently establish pulmonary tumors in mice 査読有り

Nishikawa, T., Tanaka, Y., Nishikawa, M., Kusamori, K., Mizuno, N., Mizukami, Y., Ogino, Y., Shimizu, K., Konishi, S., Takahashi, Y., Yoshinobu Takakura

Biotechnology Journal 頁： in press 2017年

Alcohol-tolerant mutants of cyanobacterium Synechococcus elongatus PCC7942 obtained by UV-C induced random mutagenesis and single cell screening system 査読有り

Arai, S*., Hayashihara, K*., Kanamoto, Y., Shimizu, K., Hirokawa, Y., Hanai, T., Murakami, A., Honda, H.

Biotechnology and Bioengineering 114 巻 ( 8 ) 頁： 1771-1778 2017年

Effective modification of cell death-inducing intracellular peptides by means of a photo-cleavable peptide array-based screening system 査読有り

Kozaki, I., Shimizu, K., Honda, H.

Journal of Bioscience and Bioengineering 124 巻 ( 2 ) 頁： 209-214 2017年

Image-based cell quality evaluation to detect irregularities under same culture process of human induced pluripotent stem cells 査読有り

Nagasaka, R., Gotou, Y., Yoshida, K., Kanie, K., Shimizu, K., Honda, H., Kato, R.

Journal of Bioscience and Bioengineering 123 巻 ( 5 ) 頁： 642-650 2017年

Optimization of Albumin Secretion and Metabolic Activity of Cytochrome P450 1A1 of Human Hepatoblastoma HepG2 Cells in Multicellular Spheroids by Controlling Spheroid Size 査読有り

Nishikawa T, Tanaka Y, Nishikawa M, Ogino Y, Kusamori K, Mizuno N, Mizukami Y, Shimizu K, Konishi S, Takahashi Y, Takakura Y.

Biological Pharmaceutical Bulletin 40 巻 ( 3 ) 頁： 334-338 2017年

A Single Cell Culture System Using Lectin-Conjugated Magnetite Nanoparticles and Magnetic Force to Screen Mutant Cyanobacteria 査読有り

Arai, S., Okochi, M., Shimizu, K., Hanai, T., Honda, H.

Arai, S., Okochi, M., Shimizu, K., Hanai, T., Honda, H. 113 巻 ( 1 ) 頁： 112-119 2016年

骨格筋組織培養用マイクロ流体チップの開発招待有り

清水一憲

バイオサイエンスとインダストリー 74 巻 ( 3 ) 頁： 224-225 2016年

Development of a tactical screening method to investigate the characteristics of functional peptides 査読有り

Kume, A., Okochi, M., Shimizu, K., Yoshida, Y., Honda, H.

Biotechnology and Bioprocess Engineering 21 巻 ( 1 ) 頁： 119-127 2016年

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