研究者詳細 - 内田　広夫

論文 - 内田　広夫

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Experimental small bowel transplantation using a newborn intestine in rats: IV. Effect of cold preservation on graft neovascularization 査読有り

H Uchida, K Tahara, T Takizawa, K Inose, T Yashiro, K Hashizume, H Ikeda, M Takahashi, E Kobayashi

JOURNAL OF PEDIATRIC SURGERY 36 巻 ( 12 ) 頁： 1805 - 1810 2001年12月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：W B SAUNDERS CO

Background/Purpose: University of Wisconsin (UW) solution is one of the most superior organ preservation solutions for liver, kidney, and pancreas; however, it still is controversial for intestinal preservation. Here, the authors studied the efficacy of preservation with 2 kinds of solutions, UW and modified TOM (m-TOM) solutions in our experimental newborn intestinal transplantation model. UW solution was used as a standard intracellular and m-TOM solution as an extracellular preservation solution. Lactated ringer (LR) solution was used as a control.
Methods. Newborn intestine, which were preserved in these solutions for 24 or 48 hours, were transplanted in the subcutaneous spaces of the syngeneic recipients without surgical vascular anastomosis and histologically examined 14 days after grafting. The preserved grafts were evaluated histologically by use of light and electron microscopy just after preservation. The biochemical parameters such as LDH and serotonin also were measured in the supernatants of preservation solutions.
Results: Fresh newborn grafts were revascularized successfully at a rate of 80% (16 of 20). After 24 hours of preservation, 65% (13 of 20), 75% (15 of 20), and 85% (17 of 20) of the grafts were observed to be revascularized in LR, m-TOM, and UW solutions, respectively. After 48 hours of preservation, 60% (12 of 20), 80% (16 of 20), and 80% (16 of 20) of the grafts also were revascularized in the respective solutions (no statistic difference among the groups). The cold-preservation did not affect the neovascularization of newborn intestine until 48 hours. Histologic findings of the preserved intestine and biochemical analyses showed that UW and m-TOM solutions kept villous architectures of the preserved grafts, however, might be harmful to enterochromaffin cells.
Conclusion: Long-time preservation of newborn intestine did not interfere with neovascularization and maturation. Copyright (C) 2001 by W.B. Saunders Company.

Web of Science

High-dose tacrolimus and lengthy survival of the combined rat pancreas/spleen graft in a high-responder combination. 査読有り

Sakuma Y, Uchida H, Nagai H, Kobayashi E

Transplant immunology 9 巻 ( 1 ) 頁： 37-42 2001年10月

High-dose tacrolimus and lengthy survival of the combined rat pancreas/spleen graft in a high-responder combination 査読有り

Y Sakuma, H Uchida, H Nagai, E Kobayashi

TRANSPLANT IMMUNOLOGY 9 巻 ( 1 ) 頁： 37 - 42 2001年10月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：ELSEVIER SCIENCE BV

Pancreas grafts do not survive for lengthy periods, especially in a high-responder rat combination. Recent data indicated that a combined spleen/pancreas graft protects against acute graft rejection and induces donor-specific tolerance. In this study, we performed a combination spleen/pancreas transplantation using high-dose tacrolimus in a high-responder rat combination of DA (RTla) to LEW(RT1(l)) and induced permanent survival in the few recipient rats. In these recipients, there was no difference in the mixed lymphocyte reaction (MLR) of the recipients when compared with that of the naive LEW splenic cells, but MLR inhibition by the serum from the recipients was significantly decreased. We also performed immunoblotting and detected a protein that has an affinity for the anti-DA class antibody. This protein may be an anti-idiotypic antibody and contribute to donor- and tissue-specific tolerance. (C) 2001 Elsevier Science BN. All rights reserved.

DOI： 10.1016/S0966-3274(01)00049-1

Web of Science

Scopus

その他リンク： http://orcid.org/0000-0002-1818-6697

Green fluorescent protein-transgenic rat: a tool for organ transplantation research. 査読有り

Hakamata Y, Tahara K, Uchida H, Sakuma Y, Nakamura M, Kume A, Murakami T, Takahashi M, Takahashi R, Hirabayashi M, Ueda M, Miyoshi I, Kasai N, Kobayashi E

Biochemical and biophysical research communications 286 巻 ( 4 ) 頁： 779-85 2001年8月

Experimental small bowel transplantation using newborn intestine in rats: III. Long-term cryopreservation of rat newborn intestine. 査読有り

Tahara K, Uchida H, Kawarasaki H, Hasizume K, Kobayashi E

Journal of pediatric surgery 36 巻 ( 4 ) 頁： 602-4 2001年4月

Experimental small bowel transplantation using newborn intestine in rats: III. Long-term cryopreservation of rat newborn intestine 査読有り

K Tahara, H Uchida, H Kawarasaki, K Hasizume, E Kobayashi

JOURNAL OF PEDIATRIC SURGERY 36 巻 ( 4 ) 頁： 602 - 604 2001年4月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：W B SAUNDERS CO

Background: If long-term organ cryopreservation can be attained, a significant achievement will have been made to address the problem for donor shortage. Fetal intestine has been known to revascularize naturally without vascular anastmosis. The authors have confirmed previously that the newborn intestine also could develop to maturity in the host omentum. Here, the authors examined whether the cryopreserved newborn intestine could revascularize in the syngeneic combination using the 2 different solutions and whether cryopreservation affect their antigenicity in the allogeneic combination.
Methods: Inbred rat strains of LEW (MHC haplotype; RT1(1)) and BN (RT1(n)) were used. LEW newborn intestinal grafts were stored in RPMI-1640 or University of Wisconsin solution with 10% DEMSO (n = 10 in each group). The grafts were placed into a cold (4 degreesC) preservation solution for 30 minutes and then placed into a freezing chamber and cooled to -80 degreesC at -1 degreesC/min after 12 hours quenched to -180 degreesC in liquid nitrogen for longer than 30 days. Then, the cryopreserved grafts under the 2 different solutions were transplanted syngenicaly (LEW to LEW). The cryopreserved BN grafts also were implanted into the LEW omentum pouch. The allotransplantation was received with a 14-day high-dose course of tacrolimus (0.64 mg/kg, intramuscularly). The grafts were evaluated histologically at 4 weeks after transplantation. Fresh newborn intestines implanted in this syngeneic and allogeneic combination were evaluated as each control group.
Result: In the syngeneic combination, more than 90% of the mature intestine were obtained. There was no significant difference among the different solution and the fresh group. However, in the allogeneic combination, both fresh and cryo preserved grafts were histologically poor.
Conclusions: This is the first report showing that long-term cryopreservation was not harmful for neovascularization of newborn intestine. Long-term cryopreservation did not reduce the antigenicity of the newborn intestine.

Web of Science

Rat auxiliary liver transplantation without portal vein reconstruction: comparison with the portal vein-arterialized model. 査読有り

Rong Xiu D, Hishikawa S, Sato M, Nagai H, Uchida H, Kobayashi E

Microsurgery 21 巻 ( 5 ) 頁： 189-95 2001年

Dosing time-dependent pharmacological effects of anti-metabolites for rat cardiac graft. 査読有り

To H, Xiu DR, Hishikawa S, Uchida H, Sudoh T, Sunaga K, Sugimoto K, Higuchi S, Fujimura A, Kobayashi E

Research communications in molecular pathology and pharmacology 110 巻 ( 5-6 ) 頁： 319-32 2001年

Orthotopic intestinal transplantation using the cuff method in rats: a histopathological evaluation of the anastomosis. 査読有り

Nakao A, Ogino Y, Tahara K, Uchida H, Kobayashi E

Microsurgery 21 巻 ( 1 ) 頁： 12-5 2001年

Simplified method of heterotopic rat heart transplantation using the cuff technique: application to sublethal dose protocol of methotrexate on allograft survival. 査読有り

Xiu D, Uchida H, To H, Sugimoto K, Kasahara K, Nagai H, Fujimura A, Kobayashi E

Microsurgery 21 巻 ( 1 ) 頁： 16-21 2001年

Chronopharmacology of oral prednisolone in rats. 査読有り

Tomizawa N, Uchida H, Xiu DR, To H, Fujimura A, Kobayashi E

Journal of medicine 32 巻 ( 3-4 ) 頁： 135-51 2001年

Simplified method of heterotopic rat heart transplantation using the cuff technique: Application to sublethal dose protocol of methotrexate on allograft survival 査読有り

Xiu DR, Uchida H, To H, Sugimoto K, Kasahara K, Nagai H, Fujimura A, Kobayashi E

MICROSURGERY 21 巻 ( 1 ) 頁： 16 - 21 2001年

Rat auxiliary liver transplantation without portal vein reconstruction: Comparison with the portal vein-arterialized model 査読有り

Xiu DR, Hishikawa S, Sato M, Nagai H, Uchida H, Kobayashi E

MICROSURGERY 21 巻 ( 5 ) 頁： 189 - 195 2001年

Orthotopic intestinal transplantation using the cuff method in rats: A histopathological evaluation of the anastomosis 査読有り

A Nakao, Y Ogino, K Tahara, H Uchida, E Kobayashi

MICROSURGERY 21 巻 ( 1 ) 頁： 12 - 15 2001年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WILEY-LISS

Segmental small intestine transplantation (SIT) in rats, using a cuff technique, has achieved a high success rate. However, there have been few reports on the influence of the foreign body reaction to polyethylene cuff on vessel anastomoses and graft after SIT. This study involves the histopathological examination of the site of cuff anastomosis and grafts in the short- and long-term survival of segmental SIT. The data obtained from the suture anastomosis model also served as a control. One week after heterotopic segmental SIT using the cuff technique, orthotopic continuations were carried out in syngeneic combination. Twenty-five of 30 rats surviving >200 days (83.3%) were examined for vessel anastomosis. All arterial anastomoses were patent, but the portovenous anastomoses in 10 grafts (33%) were totally occluded and were associated with the formation of collateral vessels. Histopathological examination demonstrated good patency of the artery and vein anastomotic site in the short term, but granulation, fibrosis, and neovascularization at the anastomosis site surrounding the cuffs in the long-surviving group. However, the grafts appeared to be intact, with normal features of the villi. On the contrary, the site of the sutured anastomosis in the long-survival rats showed no inflammatory reaction. Although a polyethylene cuff caused foreign body reaction, the graft blood supplies were maintained by collateral vessels. Considering the low mortality and high success rate, polyethylene cuff is good for short-term study and an alternative method for long-term SIT experiments. (C) 2001 Wiley-Liss, Inc.

Web of Science

Rat auxiliary liver transplantation without portal vein reconstruction: Comparison with the portal vein-arterialized model 査読有り

DR Xiu, S Hishikawa, M Sato, H Nagai, H Uchida, E Kobayashi

MICROSURGERY 21 巻 ( 5 ) 頁： 189 - 195 2001年

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：WILEY-LISS

Auxiliary liver transplantation (ALT) has been reintroduced in clinical cases recently and is now believed to be a viable alternative to orthotopic liver transplantation. To provide a simple rat ALT model for studying the physiological and immunological aspects of the ALT graft, a new ALT was performed, and the comparison between this new model and the portal arterialized one that was reported by other investigators was carried out. At first, we confirmed that liver could tolerate the deprivation of its portal flow well, using a portosystemic shunted rat model. The new rat ALT model, in which the ALT graft obtained its blood inflow only from the hepatic artery, was then performed. Our results demonstrated that 50% of the hepatic artery-alone ALT graft showed almost normal structure histologically at 1 month after grafting, with bile secretion preserved. By contrast, only 8% 1-month graft survival was noted in the portal arterialized group, and all grafts stopped bile secretion 1 week after operation. In conclusion, with arterial blood supply-alone, the ALT graft survived and demonstrated normal bile secretion function for more than 1 month. Portal vein arterialization is not an appropriate way to establish the graft's blood supply if no pressure adjustment measures were taken in advance. (C) 2001 Wiley-Liss, Inc.

Web of Science

22. 新生仔小腸を用いた実験的小腸移植の可能性(Session 2. 長期栄養管理のコツ : ここがポイントだ, 小児外科と代謝・栄養, 第 17 回日本小児外科学会秋季シンポジウム) 査読有り

内田広夫, 池田均, 田原和典, 小林英司

日本小児外科学会雑誌 37 巻 ( 6 ) 頁： 885 - 886 2001年

[Characterization of humoral immunosuppressive factors induced after allogeneic blood transfusion and their application for organ transplantation]. 査読有り

Uchida H, Tanaka H, Tahara K, Kobayashi E

Nihon Rinsho Men'eki Gakkai kaishi = Japanese journal of clinical immunology 23 巻 ( 6 ) 頁： 627-9 2000年12月

Hepatic lymphocyte transplantation in hyperbilirubinemic Gunn rats 査読有り

Y Yoshida, E Kobayashi, M Nozawa, H Uchida, A Fujimura, T Yamanaka, M Miyata

EUROPEAN SURGICAL RESEARCH 32 巻 ( 4 ) 頁： 223 - 227 2000年7月

記述言語：英語掲載種別：研究論文（学術雑誌）出版者・発行元：KARGER

Hepatic lymphomyeloid cells (HLCs) are thought to contain liver stem cells. We investigated whether HLCs generated enzyme-producing cells in vivo. HLCs from normal Wistar/Shi rats and rats in which liver ischemia was induced using a portal clamp 4 days previously were studed histopathologically and characterized using flow cytometry. Splenic lymphocytes obtained from these animals were compared as a control. The proliferative activity of HLCs and splenic cells from both groups was also tested by stimulation with concanavalin A. HLCs contained a significantly higher number of NK-T cells and OV6+ cells compared with the splenic cells. The HLCs from rats in which liver ischemia was induced tended to have greater proliferative activity than those from normal rats, while the proliferative activity of splenic lymphocytes was impaired by liver ischemia. The HLCs obtained from Wistar/Shi rats with liver ischemia were then injected into hereditary hyperbilirubinemic Gunn rats to determine whether the HLCs generated enzyme-producing cells. After injection of these stimulated HLCs, the titer of serum bilirubin in the recipient rats was markedly reduced over a long time course (6.80 +/- 0.93 to 4.87 +/- 0.22 mg/dl after 1 month and 3.52 +/- 1.33 mg/dl after 6 months). The results of the present study indicate that HLCs have different populations than splenic cells, and ischemia-reperfusion of the liver increased their proliferative activity. HLC transplantation successfully reduced high bilirubin levels over a long time course. Copyright (C) 2000 S. Karger AG, Basel.

DOI： 10.1159/000008768

Web of Science

Effect of erythromycin on ischemia-reperfusion injury of liver in rats. 査読有り

Ohmori M, Miyashita F, Uchida H, Kitoh Y, Tsuruoka S, Harada K, Sugimoto K, Fujimura A, Kobayashi E

Transplantation proceedings 32 巻 ( 4 ) 頁： 811-4 2000年6月

Effect of erythromycin on ischemia-reperfusion injury of liver in rats 査読有り

Ohmori M, Miyashita F, Uchida H, Kitoh Y, Tsuruoka S, Harada K, Sugimoto K, Fujimura A, Kobayashi E

TRANSPLANTATION PROCEEDINGS 32 巻 ( 4 ) 頁： 811 - 814 2000年6月

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