Updated on 2021/10/25
博士（医学） （ 2007.3 名古屋大学 ）
Autophagy
Regeneration
Axon
糖鎖
Life Science / Medical biochemistry
Life Science / Pathological biochemistry
Life Science / Pathophysiologic neuroscience
神経軸索再生阻害のメカニズム
Nagoya University Institute for Glyco-core Research Associate professor
2021.1
Nagoya University Associate professor
2019.10 - 2020.12
名古屋大学大学院医学系研究科 附属神経疾患・腫瘍分子医学研究センター 先端応用医学部門 助教
2016.5 - 2019.9
Country：Japan
Nagoya University
2013.4 - 2016.4
Country：Japan
名古屋大学大学院医学系研究科 生物化学講座 研究員
2012.4 - 2013.3
Country：Japan
財団法人・精神・神経科学振興財団 リサーチ・レジデント
2011.10 - 2012.3
Country：Japan
名古屋大学大学院医学系研究科 生物化学講座 研究員
2011.4 - 2011.9
Country：Japan
Nagoya University Graduate School, Division of Medical Sciences
2007.4 - 2011.3
Country： Japan
Nagoya University Faculty of Medicine
2001.4 - 2007.3
Country： Japan
日本生化学会
日本神経科学会
日本糖質学会
日本糖質学会奨励賞
2020 日本糖質学会
第6回領域班会議 最優秀発表賞
2014 新学術領域研究「神経糖鎖生物学」
つるま賞
2007 名古屋大学医学部医学科
Dermatan sulfate is an activating ligand of anaplastic lymphoma kinase. Reviewed
Machino M, Gong Y, Ozaki T, Suzuki Y, Watanabe E, Imagama S, Kadomatsu K, Sakamoto K
Journal of biochemistry 2021.7
Axonal Regeneration by Glycosaminoglycan Invited Reviewed
Sakamoto Kazuma, Ozaki Tomoya, Kadomatsu Kenji
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY Vol. 9 page： 702179 2021.6
Enoxaparin promotes functional recovery after spinal cord injury by Reviewed
Ito Sadayuki, Ozaki Tomoya, Morozumi Masayoshi, Imagama Shiro, Kadomatsu Kenji, Sakamoto Kazuma
EXPERIMENTAL NEUROLOGY Vol. 340 page： 113679 2021.6
Type IIa RPTPs and Glycans: Roles in Axon Regeneration and Synaptogenesis Invited Reviewed
Sakamoto Kazuma, Ozaki Tomoya, Suzuki Yuji, Kadomatsu Kenji
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES Vol. 22 ( 11 ) 2021.6
Identification of PTPR sigma-interacting proteins by proximity-labelling assay Reviewed
Gong Yuanhao, Abudureyimu Shaniya, Kadomatsu Kenji, Sakamoto Kazuma
JOURNAL OF BIOCHEMISTRY Vol. 169 ( 2 ) page： 187 - 194 2021.2
Glycan sulfation patterns define autophagy flux at axon tip via PTPR sigma-cortactin axis Reviewed International coauthorship
Sakamoto Kazuma, Ozaki Tomoya, Ko Yen-Chun, Tsai Cheng-Fang, Gong Yuanhao, Morozumi Masayoshi, Ishikawa Yoshimoto, Uchimura Kenji, Nadanaka Satomi, Kitagawa Hiroshi, Zulueta Medel Manuel L., Bandaru Anandaraju, Tamura Jun-ichi, Hung Shang-Cheng, Kadomatsu Kenji
NATURE CHEMICAL BIOLOGY Vol. 15 ( 7 ) page： 699 - + 2019.7
Mechanisms of axon regeneration: The significance of proteoglycans Invited Reviewed
Sakamoto Kazuma, Kadomatsu Kenji
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS Vol. 1861 ( 10 ) page： 2435 - 2441 2017.10
Mechanisms of axon regeneration and its inhibition: Roles of sulfated glycans Invited Reviewed
Kadomatsu Kenji, Sakamoto Kazuma
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS Vol. 558 page： 36 - 41 2014.9
Midkine in the pathology of cancer, neural disease, and inflammation Invited Reviewed
Sakamoto Kazuma, Kadomatsu Kenji
PATHOLOGY INTERNATIONAL Vol. 62 ( 7 ) page： 445 - 455 2012.7
Keratan Sulfate Restricts Neural Plasticity after Spinal Cord Injury Reviewed
Imagama Shiro, Sakamoto Kazuma, Tauchi Ryoji, Shinjo Ryuichi, Ohgomori Tomohiro, Ito Zenya, Zhang Haoqian, Nishida Yoshihiro, Asami Nagamasa, Takeshita Sawako, Sugiura Nobuo, Watanabe Hideto, Yamashita Toshihide, Ishiguro Naoki, Matsuyama Yukihiro, Kadomatsu Kenji
JOURNAL OF NEUROSCIENCE Vol. 31 ( 47 ) page： 17091 - 17102 2011.11
Keratan Sulfate in Neuronal Network Reconstruction Invited Reviewed
Sakamoto Kazuma, Kadomatsu Kenji
TRENDS IN GLYCOSCIENCE AND GLYCOTECHNOLOGY Vol. 23 ( 133 ) page： 212 - 220 2011.9
Premature Ligand-Receptor Interaction during Biosynthesis Limits the Production of Growth Factor Midkine and Its Receptor LDL Receptor-related Protein 1 Reviewed International coauthorship
Sakamoto Kazuma, Bu Guojun, Chen Sen, Takei Yoshifumi, Hibi Kenji, Kodera Yasuhiro, McCormick Lynn M., Nakao Akimasa, Noda Masaharu, Muramatsu Takashi, Kadomatsu Kenji
JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 286 ( 10 ) page： 8405 - 8413 2011.3
N-Acetylglucosamine 6-O-Sulfotransferase-1-Deficient Mice Show Better Functional Recovery after Spinal Cord Injury Reviewed
Ito Zenya, Sakamoto Kazuma, Imagama Shiro, Matsuyama Yukihiro, Zhang Haoqian, Hirano Kenichi, Ando Kei, Yamashita Toshihide, Ishiguro Naoki, Kadomatsu Kenji
JOURNAL OF NEUROSCIENCE Vol. 30 ( 17 ) page： 5937 - 5947 2010.4
Axonal regeneration and keratan sulfate Reviewed
Kadomatsu Kenji, Imagama Shiro, Ito Zenya, Sakamoto Kazuma, Matsuyama Yukihiro
NEUROSCIENCE RESEARCH Vol. 61 page： S17 - S17 2008
Midkine and LDL-receptor-related protein 1 contribute to the anchorage-independent cell growth of cancer cells
Chen Sen, Bu Guojun, Takei Yoshifumi, Sakamoto Kazuma, Ikematsu Shinya, Muramatsu Takashi, Kadomatsu Kenji
JOURNAL OF CELL SCIENCE Vol. 120 ( 22 ) page： 4009 - 4015 2007.11
Midkine Interaction with Chondroitin Sulfate Model Synthetic Tetrasaccharides and Their Mimetics: The Role of Aromatic Interactions Reviewed International coauthorship
Garcia-Jimenez Maria Jose, Gil-Caballero Sergio, Maza Susana, Corzana Francisco, Juarez-Vicente Francisco, Miles Jonathan R., Sakamoto Kazuma, Kadomatsu Kenji, Garcia-Dominguez Mario, de Paz Jose L., Nieto Pedro M.
CHEMISTRY-A EUROPEAN JOURNAL Vol. 27 ( 48 ) page： 12395 - 12409 2021.8
Generation and characterization of antagonistic anti-human interleukin (IL)-18 monoclonal antibodies with high affinity: Two types of monoclonal antibodies against full-length IL-18 and the neoepitope of inflammatory caspase-cleaved active IL-18 Reviewed
Nariai Yuko, Kamino Hiroki, Obayashi Eiji, Kato Hiroaki, Sakashita Gyosuke, Sugiura Tomoko, Migita Kiyoshi, Koga Tomohiro, Kawakami Atsushi, Sakamoto Kazuma, Kadomatsu Kenji, Nakakido Makoto, Tsumoto Kouhei, Urano Takeshi
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS Vol. 663 page： 71 - 82 2019.3
Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice Reviewed
Takemoto Yoshio, Horiba Mitsuru, Harada Masahide, Sakamoto Kazuma, Takeshita Kyosuke, Murohara Toyoaki, Kadomatsu Kenji, Kamiya Kaichiro
CIRCULATION JOURNAL Vol. 82 ( 1 ) page： 19 - 27 2018.1
Neurocan, an extracellular chondroitin sulfate proteoglycan, stimulates neuroblastoma cells to promote malignant phenotypes
Su Zhendong, Kishida Satoshi, Tsubota Shoma, Sakamoto Kazuma, Cao Dongliang, Kiyonari Shinichi, Ohira Miki, Kamijo Takehiko, Narita Atsushi, Xu Yinyan, Takahashi Yoshiyuki, Kadomatsu Kenji
ONCOTARGET Vol. 8 ( 63 ) page： 106296 - 106310 2017.12
Dissecting the interaction between tissue inhibitor of metalloproteinases-3 (TIMP-3) and low density lipoprotein receptor-related protein-1 (LRP-1): Development of a "TRAP" to increase levels of TIMP-3 in the tissue Reviewed International coauthorship
Scilabra Simone D., Yamamoto Kazuhiro, Pigoni Martina, Sakamoto Kazuma, Mueller Stephan A., Papadopoulou Alkmini, Lichtenthaler Stefan F., Troeberg Linda, Nagase Hideaki, Kadomatsu Kenji
MATRIX BIOLOGY Vol. 59 page： 69 - 79 2017.5
Therapeutic potential of midkine in cardiovascular disease Invited Reviewed
Kadomatsu Kenji, Bencsik Peter, Goerbe Aniko, Csonka Csaba, Sakamoto Kazuma, Kishida Satoshi, Ferdinandy Peter
BRITISH JOURNAL OF PHARMACOLOGY Vol. 171 ( 4 ) page： 936 - 944 2014.2
Keratan sulfate expression in microglia is diminished in the spinal cord in experimental autoimmune neuritis Reviewed
Matsui H., Ohgomori T., Natori T., Miyamoto K., Kusunoki S., Sakamoto K., Ishiguro N., Imagama S., Kadomatsu K.
CELL DEATH & DISEASE Vol. 4 2013.12
Ablation of Keratan Sulfate Accelerates Early Phase Pathogenesis of ALS Reviewed
Hirano Kenichi, Ohgomori Tomohiro, Kobayashi Kazuyoshi, Tanaka Fumiaki, Matsumoto Tomohiro, Natori Takamitsu, Matsuyama Yukihiro, Uchimura Kenji, Sakamoto Kazuma, Takeuchi Hideyuki, Hirakawa Akihiro, Suzumura Akio, Sobue Gen, Ishiguro Naoki, Imagama Shiro, Kadomatsu Kenji
PLOS ONE Vol. 8 ( 6 ) page： e66969 2013.6
Minocycline selectively inhibits M1 polarization of microglia Reviewed
Kobayashi K., Imagama S., Ohgomori T., Hirano K., Uchimura K., Sakamoto K., Hirakawa A., Takeuchi H., Suzumura A., Ishiguro N., Kadomatsu K.
CELL DEATH & DISEASE Vol. 4 2013.3
Midkine Promotes Neuroblastoma through Notch2 Signaling Reviewed
Kishida Satoshi, Mu Ping, Miyakawa Shin, Fujiwara Masatoshi, Abe Tomoyuki, Sakamoto Kazuma, Onishi Akira, Nakamura Yoshikazu, Kadomatsu Kenji
CANCER RESEARCH Vol. 73 ( 4 ) page： 1318 - 1327 2013.2
Human dental pulp-derived stem cells promote locomotor recovery after complete transection of the rat spinal cord by multiple neuro-regenerative mechanisms Reviewed
Sakai Kiyoshi, Yamamoto Akihito, Matsubara Kohki, Nakamura Shoko, Naruse Mami, Yamagata Mari, Sakamoto Kazuma, Tauchi Ryoji, Wakao Norimitsu, Imagama Shiro, Hibi Hideharu, Kadomatsu Kenji, Ishiguro Naoki, Ueda Minoru
JOURNAL OF CLINICAL INVESTIGATION Vol. 122 ( 1 ) page： 80 - 90 2012.1
Basigin/CD147 Promotes Renal Fibrosis after Unilateral Ureteral Obstruction Reviewed
Kato Noritoshi, Kosugi Tomoki, Sato Waichi, Ishimoto Takuji, Kojima Hiroshi, Sato Yuka, Sakamoto Kazuma, Maruyama Shoichi, Yuzawa Yukio, Matsuo Seiichi, Kadomatsu Kenji
AMERICAN JOURNAL OF PATHOLOGY Vol. 178 ( 2 ) page： 572 - 579 2011.2
DRR1 is expressed in the developing nervous system and downregulated during neuroblastoma carcinogenesis Reviewed
Asano Yoshizumi, Kishida Satoshi, Mu Ping, Sakamoto Kazuma, Murohara Toyoaki, Kadomatsu Kenji
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Vol. 394 ( 3 ) page： 829 - 835 2010.4
The E-Selectin Ligand Basigin/CD147 Is Responsible for Neutrophil Recruitment in Renal Ischemia/Reperfusion Reviewed
Kato Noritoshi, Yuzawa Yukio, Kosugi Tomoki, Hobo Akinori, Sato Waichi, Miwa Yuko, Sakamoto Kazuma, Matsuo Seiichi, Kadomatsu Kenji
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY Vol. 20 ( 7 ) page： 1565 - 1576 2009.7
Transforming growth factor-beta 1 upregulates keratan sulfate and chondroitin sulfate biosynthesis in microglias after brain injury Reviewed
Yin Jiarong, Sakamoto Kazuma, Zhang Haoqian, Ito Zenya, Imagama Shiro, Kishida Satoshi, Natori Takamitsu, Sawada Makoto, Matsuyama Yukihiro, Kadomatsu Kenji
BRAIN RESEARCH Vol. 1263 page： 10 - 22 2009.3
実験医学増刊 第三の生命鎖 糖鎖の機能と疾患 「硫酸化糖鎖と神経回路再編」
坂元一真, 内村健治, 門松健治（ Role： Joint author）
羊土社 2013.6
硫酸化糖鎖によるDystrophic endball形成 機構
坂元一真 門松健治（ Role： Joint author）
日本生化学会 2020
神経軸索におけるオートファジーとその破綻
坂元一真 門松健治（ Role： Joint author）
日本生化学会 2018
グリコサミノグリカンと疾病
尾﨑 智也，坂元 一真，内村 健治，門松 健治（ Role： Joint author）
日本生化学会 2017
Dystrophic endball形成および軸索再生阻害における硫酸化糖鎖の役割 Invited
坂元一真、門松健治
第86回日本生化学会大会 2014
Glycans and dystrophic endball Invited International conference
Kazuma Sakamoto
Glyco-Neuroscience Workshop 2019.5 Acdemia Sinica
Dystrophic endball形成の細胞生物学的基盤 Invited
坂元一真 門松健治
第123回日本解剖学会総会・全国学術集会 2018
一般財団法人共済団医学研究奨励助成
2016.4 - 2017.3
学内共同研究
公益財団法人堀科学芸術振興財団研究助成
2014.4 - 2015.3
学内共同研究
オートクラインループにおける小胞体シャペロンの意義
2008.4 - 2011.3
科学研究費補助金
Authorship：Principal investigator
Establishment of substrates for receptor tyrosine phosphatase and its application
Grant number：19K07348 2019.4 - 2022.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Authorship：Principal investigator
Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）
Grant number：16K19049 2016.4 - 2018.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
Sakamoto Kazuma
Authorship：Principal investigator
Grant amount：\3900000 （ Direct Cost: \3000000 、 Indirect Cost：\900000 ）
Upon injury, the distal terminal of axon transforms into so-called dystrophic endball. This degenerative structure has been thought to be closely associated with inability of regeneration of our axons in the cetral nervous system. However, underlying molecular mechanism except neuronal cell-surface molecule, PTPRσ, has been unknown. Here I have identified Cortactin as a new substrate for PTPRσ. Cortactin was de-phosphorylated by PTPRσ, and its phosphorylated form was completely diminished in dystrophic endball. Furthermore, knock-down of Cortactin in cultured neurons led to inhibition of axonal elongation, and swollen structures with autophagosomal accumulation which was specific character of dystrophic endball.
Taken together, these results have revealed a pivotal role of PTPRσ-Cortactin axis in axonal regeneration and its inhibition.
Neuronal cell surface mechanisms which induce dystrophic endball
Grant number：26860209 2014.4 - 2016.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
Sakamoto Kazuma
Authorship：Principal investigator
Grant amount：\3900000 （ Direct Cost: \3000000 、 Indirect Cost：\900000 ）
Heparan sulfate and chondroitin sulfate are sulfated glycans which are involved in axonal elongation in our central nervous system. Although they share the same neuronal receptor PTPσ, how they differentially regulate it is still unknown. Here, minimum essential structures of heparan sulfate and chondroitin sulfate were successfully identified. In both glycans, tetrasaccharide is the shortest structure to interact with PTPσ. However, interestingly, the probability of the structures is quite rare in chondroitin sulfate, while quite rich in heparan sulfate. These findings account for how heparan sulfate and chondroitin sulfate work in opposing manner on PTPσ and axonal elongation.
Identification of receptors for phosphacan which inhibit axonal regeneration
Grant number：24890084 2012.8 - 2014.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Research Activity Start-up
SAKAMOTO Kazuma
Authorship：Principal investigator
Grant amount：\2600000 （ Direct Cost: \2000000 、 Indirect Cost：\600000 ）
Highly purified recombinant phosphacan was purepared using anion exchange and gel filtration chromatography. Increasing gradient of recombinant phosphacan induced dystrophic endball, a morphological marker for regeneration stopping axons, to cultured dorsal root ganglion neurons. Using electron microscope and immunocytochemistry, autophagosomes were found in dystrophic endball, suggesting that autophagy is an essential cellular event for formation of dystrophic endball.
Surface plasmon resonance assay revealed that phosphacan strongly bound to PTPsigma and LAR which are known as receptors for chondroitin sulfate. Surprisingly, interaction between phosphacan and PTPsigma or LAR was two-phase manner, and not only chondroitin sulfate but also keratan sulfate were involved in interaction. Free keratan sulfate sugar also bound to PTPsigma and LAR.
PTPzeta, another member of receptors tyrosine phosphatase, was also identified to be involved in inhibition of axonal regeneration.
A new ligand for ALK and its activation mechanism
Grant number：19H03415 2019.4 - 2022.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)
Authorship：Coinvestigator(s) Grant type：Competitive
The intracellular signaling of Midkine in neuroblastoma
Grant number：16K08617 2016.4 - 2019.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Kishida Satoshi
Authorship：Coinvestigator(s) Grant type：Competitive
Midkine, a secreted growth factor, was shown to be involved in cisplatin resistance of bone marrow-metastasized neuroblastoma cells. The midkine-targetting therapy together with cisplatin could efficiently kill bone marrow-metastasized neuroblastma cells. We also found that mouse embryonic fibroblast (MEF) remarkably responded to midkine. This result suggests that midkine would signal not only to neuroblastoma cells but also stromal cells including fibroblasts.
基礎医学セミナー
2017
生物の化学
2017
生物の化学 実習
2017
生物の化学
2016
生物の化学 実習
2016
基礎医学セミナー
2016
基礎医学セミナー
2021
生物の化学 実習
2021
生物学基礎
2021
生物の化学 講義
2021
基礎医学セミナー
2020
生物学基礎
2020
生物の化学 実習
2020
生物の化学 講義
2020
生物の化学 講義
2019
生物学基礎
2019
基礎医学セミナー
2019
生物の化学 実習
2019
生物の化学 講義
2018
基礎医学セミナー
2018
生物の化学 実習
2018
プレスリリース Newspaper, magazine
2019.5
第95回日本生化学会大会 幹事補佐
Role(s)：Planning, management, etc.
日本生化学会 2021.3
日本生化学会中部支部幹事
Role(s)：Planning, management, etc.
日本生化学会 2018.9 - 2020.8
J Biochemistry誌 Editorial Assistant International contribution
Role(s)：Review, evaluation, Peer review
日本生化学会 2018
