Updated on 2022/04/11

写真a

 
HAYASHI Yumi
 
Organization
Graduate School of Medicine Lecturer
Graduate School
Graduate School of Medicine
Undergraduate School
School of Health Sciences
Title
Lecturer
Contact information
メールアドレス

Degree 1

  1. 博士(医学) ( 2012.3   名古屋大学 ) 

Research Areas 3

  1. Life Science / Hygiene and public health (laboratory)  / DOHaD

  2. Life Science / Nutrition science and health science  / 妊娠期低栄養

  3. Life Science / Nutrition science and health science  / メタボロミクス

Research History 2

  1. Nagoya University   Institute for Advanced Research

    2018.4

  2. Nagoya University   Institute for Advanced Research

    2014.12 - 2018.3

Education 2

  1. Nagoya University   Graduate School, Division of Medical Sciences

    2009.4 - 2012.3

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    Country: Japan

  2. Nagoya University   Graduate School, Division of Medical Sciences

    2007.4 - 2009.3

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    Country: Japan

Awards 2

  1. Royal Society of Chemistry Tokyo International Conference 2016 Best Poster Award

    2016.9   Royal Society of Chemistry   Intact metabolome analysis of mice biological tissues by probe electrospray ionization-tandem mass spectrometry (PESI-MS/MS) and its application to real-time analysis

    Yumi Hayashi, Kei Zaitsu, Tasuku Murata, Hiroki Nakajima, Maiko Kusano, Hitoshi Tsuchihashi, Akira Ishii, Tetsuya Ishikawa

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    Award type:Award from international society, conference, symposium, etc.  Country:Japan

  2. 若手優秀ポスター賞

    2015.9   日本医用マススペクトル学会  

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    Country:Japan

 

Papers 43

  1. Association of self-reported moderate vegetable juice intake with small decline in kidney function in a five-year prospective study

    Fujii Ryosuke, Kondo Takaaki, Tsukamoto Mineko, Kawai Sayo, Sasakabe Tae, Naito Mariko, Kubo Yoko, Okada Rieko, Tamura Takashi, Hishida Asahi, Ueyama Jun, Hayashi Yumi, Ohnishi Joji, Hamajima Nobuyuki, Takeuchi Kenji, Wakai Kenji

    NUTRITION   Vol. 84   page: 111114   2021.4

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  2. RECiQ: A Rapid and Easy Method for Determining Cyanide Intoxication by Cyanide and 2-Aminothiazoline-4-carboxylic Acid Quantification in the Human Blood Using Probe Electrospray Ionization Tandem Mass Spectrometry

    Hisatsune Kazuaki, Murata Tasuku, Ogata Koretsugu, Hida Minemasa, Ishii Akira, Tsuchihashi Hitoshi, Hayashi Yumi, Zaitsu Kei

    ACS OMEGA   Vol. 5 ( 36 ) page: 23351 - 23357   2020.9

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  3. A preliminary study of rapid-fire high-throughput metabolite analysis using nano-flow injection/Q-TOFMS

    Taki Kentaro, Noda Saki, Hayashi Yumi, Tsuchihashi Hitoshi, Ishii Akira, Zaitsu Kei

    ANALYTICAL AND BIOANALYTICAL CHEMISTRY   Vol. 412 ( 17 ) page: 4127 - 4134   2020.7

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  4. Short-term high-fat diet intake leads to exacerbation of concanavalin A-induced liver injury through the induction of procoagulation state

    Nanizawa Eri, Tamaki Yuki, Sono Reika, Miyashita Rintaro, Hayashi Yumi, Kanbe Ayumu, Ito Hiroyasu, Ishikawa Tetsuya

    BIOCHEMISTRY AND BIOPHYSICS REPORTS   Vol. 22   page: 100736   2020.7

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  5. In utero exposure to di(2-ethylhexyl)phthalate suppresses blood glucose and leptin levels in the offspring of wild-type mice.

    Hayashi Y, Ito Y, Naito H, Tamada H, Yamagishi N, Kondo T, Ishikawa T, Gonzalez FJ, Nakajima T

    Toxicology   Vol. 415   page: 49 - 55   2019.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.tox.2019.01.008

    Web of Science

    PubMed

  6. Intact metabolite profiling of mouse brain by probe electrospray ionization/triple quadrupole tandem mass spectrometry (PESI/MS/MS) and its potential use for local distribution analysis of the brain (vol 983, pg 160, 2017)

    Hayashi Yumi, Zaitsu Kei, Murata Tasuku, Ohara Tomomi, Moreau Stephane, Kusano Maiko, Tanihata Hiroshi, Tsuchihashi Hitoshi, Ishii Akira, Ishikawa Tetsuya

    ANALYTICA CHIMICA ACTA   Vol. 1031   page: 196 - 196   2018.11

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  7. Metabolome analysis of the serotonin syndrome rat model: Abnormal muscular contraction is related to metabolic alterations and hyper-thermogenesis

    Zaitsu Kei, Noda Saki, Iguchi Akira, Hayashi Yumi, Ohara Tomomi, Kimura Yuya, Koketsu Yuta, Kosaki Tomoko, Kusano Maiko, Sato Takako, Ishikawa Tetsuya, Tsuchihashi Hitoshi, Suzuki Koichi, Ishii Akira

    LIFE SCIENCES   Vol. 207   page: 550-561   2018.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.lfs.2018.06.031

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  8. In Vivo Real-Time Monitoring System Using Probe Electrospray Ionization/Tandem Mass Spectrometry for Metabolites in Mouse Brain

    Zaitsu Kei, Hayashi Yumi, Murata Tasuku, Yokota Kazumi, Ohara Tomomi, Kusano Maiko, Tsuchihashi Hitoshi, Ishikawa Tetsuya, Ishii Akira, Ogata Koretsugu, Tanihata Hiroshi

    ANALYTICAL CHEMISTRY   Vol. 90 ( 7 ) page: 4695-4701   2018.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.analchem.7b05291

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  9. Bile acid detoxifying enzymes limit susceptibility to liver fibrosis in female SHRSP5/Dmcr rats fed with a high-fat-cholesterol diet

    Yetti Husna, Naito Hisao, Yuan Yuan, Jia Xiaofang, Hayashi Yumi, Tamada Hazuki, Kitamori Kazuya, Ikeda Katsumi, Yamori Yukio, Nakajima Tamie

    PLOS ONE   Vol. 13 ( 2 ) page: e0192863   2018.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1371/journal.pone.0192863

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  10. Fatal intoxication by 5F-ADB and diphenidine: Detection, quantification, and investigation of their main metabolic pathways in humans by LC/MS/MS and LC/Q-TOFMS

    Kusano Maiko, Zaitsu Kei, Taki Kentaro, Hisatsune Kazuaki, Nakajima Jun'ichi, Moriyasu Takako, Asano Tomomi, Hayashi Yumi, Tsuchihashi Hitoshi, Ishii Akira

    DRUG TESTING AND ANALYSIS   Vol. 10 ( 2 ) page: 284-293   2018.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/dta.2215

    Web of Science

    PubMed

  11. Intact metabolite profiling of mouse brain by probe electrospray ionization/triple quadrupole tandem mass spectrometry (PESI/MS/MS) and its potential use for local distribution analysis of the brain

    Hayashi Yumi, Zaitsu Kei, Murata Tasuku, Ohara Tomomi, Moreau Stephane, Kusano Maiko, Tanihata Hiroshi, Tsuchihashi Hitoshi, Ishii Akira, Ishikawa Tetsuya

    ANALYTICA CHIMICA ACTA   Vol. 983   page: 160-165   2017.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.aca.2017.06.047

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    PubMed

  12. Intact metabolite profiling of mouse brain by probe electrospray ionization/triple quadrupole tandem mass spectrometry (PESI/MS/MS) and its potential use for local distribution analysis of the brain. Reviewed

    HAYASHI Yumi, ZAITSU Kei, MURATA Tasuku, OHARA Tomomi, MOREAU Stéphane, KUSANO Maiko, TANIHATA Hiroshi, TSUCHIHASHI Hitoshi, ISHII Akira, ISHIKAWA Tetsuya.

    Anal Chim Acta   Vol. 983   page: 160-165   2017.8

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    Language:English   Publishing type:Research paper (scientific journal)  

  13. Fatal intoxication by 5F-ADB and diphenidine: Detection, quantification, and investigation of their main metabolic pathways in humans by LC/MS/MS and LC/Q-TOFMS Reviewed

    KUSANO Maiko, ZAITSU Kei, TAKI Kentaro, HISATSUNE Kazuaki, NAKAJIMA Jun'ichi, MORIYASU Takako, ASANO Tomomi, HAYASHI Yumi, TSUCHIHASHI Hitoshi, ISHII Akira.

    Drug Test Anal     page: 1-10   2017.5

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    Language:English   Publishing type:Research paper (scientific journal)  

  14. Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells on Concanavalin A-Induced Acute Liver Injury in Mice.

    Yoshizumi Y, Yukawa H, Iwaki R, Fujinaka S, Kanou A, Kanou Y, Yamada T, Nakagawa S, Ohara T, Nakagiri K, Ogihara Y, Tsutsui Y, Hayashi Y, Ishigami M, Baba Y, Ishikawa T

    Cell medicine   Vol. 9 ( 1-2 ) page: 21-33   2017.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3727/215517916X693159

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  15. Immunomodulatory Effects of Adipose Tissue-Derived Stem Cells on Concanavalin A-Induced Acute Liver Injury in Mice. Reviewed

    YOSHIZUMI Yasuma, YUKAWA Hiroshi, IWAKI Ryoji, FUJINAKA Sanae, KANOU Ayano, KANOU Yuki, YAMADA Tatsuya, NAKAGAWA Shingo, OHARA Tomomi, NAKAGIRI Kenta, OGIHARA Yusuke, TSUTSUI Yoko, HAYASHI Yumi, ISHIGAMI Masatoshi, BABA Yoshinobu, ISHIKAWA Tetsuya

    Cell Medicine   Vol. 9 ( 1-2 ) page: 21-33   2016.10

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  16. Importance of detoxifying enzymes in differentiating fibrotic development between SHRSP5/Dmcr and SHRSP rats. Reviewed

    NAITO Hisao, JIA Xiaofang, YETTI Husna, YANAGIBA Yukie, TAMADA Hazuki, KITAMORI Kazuya, HAYASHI Yumi, WANG Dong, KATO Masashi, ISHII Akira, NAKAJIMA Tamie.

    Environmental Health and Preventive Medicine     page: in press   2016.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s12199-016-0539-x

  17. Development of a mass spectrometric hydroxyl-position determination method for the hydroxyindole metabolites of JWH-018 by GC-MS/MS. Reviewed

    KUSANO Maiko, ZAITSU Kei, YAMANAKA Mayumi, HISATSUNE Kazuaki, ASANO Tomomi, TAKI Kentaro, HAYASHI Yumi, TSUCHIHASHI Hitoshi, ISHII Akira.

    Journal of mass spectrometry   Vol. 51 ( 5 ) page: 350-357   2016.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/jms.3761.

  18. Intact Endogenous Metabolite Analysis of Mice Liver by Probe Electrospray Ionization/Triple Quadrupole Tandem Mass Spectrometry and Its Preliminary Application to in Vivo Real-Time Analysis Reviewed

    ZAITSU Kei, HAYASHI Yumi, MURATA Tasuku, OHARA Tomomi, NAKAGIRI Kenta, KUSANO Maiko, NAKAJIMA Hiroki, NAKAJIMA Tamie, ISHIKAWA Tetsuya, TSUCHIHASHI Hitoshi, ISHII Akira

    Analytical Chemistry   Vol. 88 ( 7 ) page: 3556-3561   2016.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1021/acs.analchem.5b04046

  19. Malondialdehyde induces autophagy dysfunction and VEGF secretion in the retinal pigment epithelium in age-related macular degeneration Reviewed

    Fuxiang Ye, Hiroki Kaneko, Yumi Hayashi, Kei Takayama; Shiang-Jyi Hwang; Yuji Nishizawa, Reona Kimoto, Yosuke Nagasaka, Taichi Tsunekawa, Toshiyuki Matsuura, Tsutomu Yasukawa, Takaaki Kondo, Hiroko Terasaki

    Free Radical Biology & Medicine     page: im press   2016

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  20. Nanoparticle-rich diesel exhaust-induced liver damage via inhibited transactivation of peroxisome proliferator-activated receptor alpha. Reviewed

    ITO Yuki, YANAGIBA Yukie, RAMDHAN Doni Hikmat, HAYASHI Yumi, LI Yufei, SUZUKI Akira K, KAMIJIMA Michihiro, NAKAJIMA Tamie

    Environ Toxicol     2015.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/tox.22199

  21. High-resolution mass spectrometric determination of the synthetic cannabinoids MAM-2201, AM-2201, AM-2232, and their metabolites in postmortem plasma and urine by LC/Q-TOFMS. Reviewed

    ZAITSU Kei, NAKAYAMA Hiroshi, YAMANAKA Mayumi, HISATSUNE Kazuaki, TAKI Kentaro, ASANO Tomomi, KAMATA Tooru, KATAGAI Munehiro, HAYASHI Yumi, KUSANO Maiko, TSUCHIHASHI Hitoshi, ISHII Akira.

    Int J Legal Med   Vol. 129 ( 6 ) page: 1233-1245   2015.9

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  22. Association of maternal whole blood fatty acid status during the prenatal period with term birth dimensions: a cross-sectional study. Reviewed

    JIA Xiaofang, TAGAWA Masahiro, YATSUYA Hiroshi, NAITO Hisao, HAYASHI Yumi, YETTI Husna, SASAKI Seiko, ARAKI Atsuko, MIYASHITA Chihiro, IKENO Tamiko, KISHI Reiko, NAKAJIMA Tamie.

    J Perinat Med.   Vol. 43 ( 5 ) page: 565-575   2015

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  23. Prenatal maternal blood triglyceride and fatty acid levels in relation to exposure to di(2-ethylhexyl)phthalate: a cross-sectional study.

    JIA Xiaofang, HARADA Yukiko, TAGAWA Masahiro, NAITO Hisao, HAYASHI Yumi, YETTI Husna, KATO Masashi, SASAKI Seiko, ARAKI Atsuko, MIYASHITA Chihiro, IKENO Tamiko, KISHI Reiko, NAKAJIMA Tamie.

    Environ Health Prev Med.   Vol. 3   page: 168-178   2015

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  24. Metabolome disruption of the rat cerebrum induced by the acute toxic effects of the synthetic cannabinoid MAM-2201. Reviewed

    ZAITSU Kei, HAYASHI Yumi SUZUKI Kei, NAKAYAMA Hiroshi, HATTORI Nanpei, TAKAHARA Rina, KUSANO Maiko, TSUCHIHASHI Hitoshi, ISHII Akira.

    Life Sci.   Vol. 137   page: 49-55   2015

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  25. Prenatal Exposure to Di(2-ethylhexyl) phthalate and Subsequent Infant and Child Health Effects. Reviewed

    NAKAJIMA Tamie, HAYASHI Yumi, ITO Yuki

    Food Safety   Vol. 3 ( 3 ) page: 70-83   2015

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  26. Peroxisome proliferator-activated receptor α mediates di-(2-ethylhexyl) phthalate transgenerational repression of ovarian Esr1 expression in female mice Reviewed

    Michihiro Kawano, Xian-Yang Qin, Midori Yoshida, Tomokazu Fukuda, Hiroko Nansai, Yumi Hayashi, Tamie Nakajima, Hideko Sone

    Toxicology Letters   Vol. 228 ( 3 ) page: 235-240   2014.5

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  27. Novel positively charged nanoparticle labeling for in vivo imaging of adipose tissue-derived stem cells.

    YUKAWA Hiroshi, NAKAGAWA Shingo, YOSHIZUMI Yasuma, WATANABE Masaki, SAITO Hiroaki, MIYAMOTO Yoshitaka, NOGUCHI Hirofumi, OISHI Koichi, ONO Kenji, SAWADA Makoto, KATO Ichiro, ONOSHIMA Daisuke, OBAYASHI Momoko, HAYASHI Yumi, KAJI Noritada, ISHIKAWA Tetsuya, HAYASHI Shuji, BABA Yoshinobu.

    PLoS One.   Vol. 9 ( 11 ) page: e110142   2014

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  28. 10. Effects of exposure to Di(2-ethylhexyl)phthalate during fetal period on next generation. Reviewed

    HAYASHI Yumi, ITO Yuki, NAKAJIMA Tamie.

      Vol. 69 ( 2 ) page: 86-91   2014

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    Language:Japanese  

  29. A revised method for determination of dialkylphosphate levels in human urine by solid-phase extraction and liquid chromatography with tandem mass spectrometry: application to human urine samples from Japanese children.

    UEYAMA Jun, SAITO Isao, TAKAISHI Ayuko, NOMURA Hiroshi, INOUE Mai, OSAKA Aya, SUGIURA Yuka, HAYASHI Yumi, WAKUSAWA Shinya, OGI Hiroko, INUZUKA Kimio, KAMIJIMA Michihiro, KONDO Takaaki.

    Environ Health Prev Med.   Vol. 19 ( 6 ) page: 405-413   2014

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  30. Species and inter-individual differences in metabolic capacity of di(2-ethylhexyl)phthalate (DEHP) between human and mouse livers. Reviewed

    Ito Y, Kamijima M, Hasegawa C, Tagawa M, Kawai T, Miyake M, Hayashi Y, Naito H, Nakajima T.

    Environmental Health and Preventive Medicine     2013.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s12199-013-0362-6

  31. High-fat-cholesterol diet-induced mainly necrosis in fibrotic steatohepatitis rats by suppressing caspase activity. Reviewed

    Yetti H, Naito H, Jia X, Shindo M, Taki H, Tamada H, Kitamori K, Hayashi Y, Ikeda K, Yamori Y, Nakajima T

    Life Sciences   Vol. 93 ( 18-19 ) page: 673-680   2013.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.lfs.2013.09.013

  32. Dysregulated bile acid synthesis, metabolism and excretion in a high fat-cholesterol diet-induced fibrotic steatohepatitis in rats. Reviewed

    Jia X, Naito H, Yetti H, Tamada H, Kitamori K, Hayashi Y, Wang D, Yanagiba Y, Wang J, Ikeda K, Yamori Y, Nakajima T

    Digestive Diseases and Sciences   Vol. 58 ( 8 ) page: 2212-2222   2013.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10620-013-2747-1.

  33. Exposure to DEHP decreased four fatty acid levels in plasma of prepartum mice. Reviewed

    Nakashima R, Hayashi Y, Md K, Jia X, Wang D, Naito H, Ito Y, Kamijima M, Gonzalez FJ, Nakajima T

    Toxicology     2013.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.tox.2013.04.010.

  34. Effect of nanoparticle-rich diesel exhaust on testicular and hippocampus steroidogenesis in male rats.

    Yamagishi N, Ito Y, Ramdhan DH, Yanagiba Y, Hayashi Y, Wang D, Li CM, Taneda S, Suzuki AK, Taya K, Watanabe G, Kamijima M, Nakajima T.

    Inhal Toxicol   Vol. 24 ( 8 ) page: 459-467   2012.6

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  35. Differences in metabolite burden of di(2-ethylhexyl)phthalate in pregnant and postpartum dams and their offspring in relation to drug-metabolizing enzymes in mice.

    Hayashi Y, Ito Y, Yanagiba Y, Kamijima M, Naito H, Nakajima T.

    Arch Toxicol   Vol. 86 ( 4 ) page: 563-569   2012.4

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  36. The modulation of hepatic adenosine triphosphate and inflammation by eicosapentaenoic acid during severe fibrotic progression in the SHRSP5/Dmcr rat model.

    Jia X, Naito H, Yetti H, Tamada H, Kitamori K, Hayashi Y, Yamagishi N, Wang D, Yanagiba Y, Ito Y, Wang J, Tanaka N, Ikeda K, Yamori Y, Nakajima T.

    Life Sci   Vol. 90 ( 23-24 ) page: 934-943   2012.1

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  37. Modulation of ammonium perfluorooctanoate-induced hepatic damage by genetically different PPARα in mice

    Nakagawa T, Ramdhan DH, Tanaka N, Naito H, Tamada H, Ito Y, Li Y, Hayashi Y, Yamagishi N, Yanagiba Y, Aoyama T, Gonzalez FJ, Nakajima T.

    Arch Toxicol   Vol. 86 ( 1 ) page: 63-74   2012.1

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  38. 胎生期フタル酸ジ-2-エチルヘキシル曝露による低栄養環境と生活習慣病

    林 由美, 伊藤 由起, 那須 民江

    日本衛生学雑誌   Vol. 67 ( 1 ) page: 22-25   2012

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  39. Ammonium perfluorooctanoate may cause testosterone reduction by adversely affecting testis in relation to PPARα.

    Nakagawa T, Ramdhan DH, Tanaka N, Naito H, Tamada H, Ito Y, Li Y, Hayashi Y, Yamagishi N, Yanagiba Y, Aoyama T, Gonzalez FJ, Nakajima T.

    Toxicol Lett   Vol. 205 ( 3 ) page: 265-272   2011.9

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  40. Hepatic peroxisome proliferator-activated receptor α may have an important role in the toxic effects of di(2-ethylhexyl)phthalate on offspring of mice

    Hayashi Y, Ito Y, Yamagishi N, Yanagiba Y, Tamada H, Wang D, Ramdhan DH, Naito H, Harada Y, Kamijima M, Gonzales FJ, Nakajima T.

    Toxicology   Vol. 289 ( 1 ) page: 1-10   2011.2

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  41. Differential response to trichloroethylene-induced hepatosteatosis in wild-type and PPARalpha-humanized mice

    Ramdhan DH, Kamijima M, Wang D, Ito Y, Naito H, Yanagiba Y, Hayashi Y, Tanaka N, Aoyama T, Gonzalez FJ, Nakajima T.

    Environ Health   Vol. 118 ( 11 ) page: 1557-1563   2010.8

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  42. Microgram-order ammonium perfluorooctanoate may activate mouse peroxisome proliferator-activated receptor alpha, but not human PPARalpha

    Nakamura T, Ito Y, Yanagiba Y, Ramdhan DH, Kono Y, Naito H, Hayashi Y, Li Y, Aoyama T, Gonzalez FJ, Nakajima T.

    Toxicology   Vol. 265 ( 1-2 ) page: 27-33   2009.9

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  43. Nanoparticle-rich diesel exhaust may disrupt testosterone biosynthesis and metabolism via growth hormone Reviewed

    Ramdhan DH, Ito Y, Yanagiba Y, Yamagishi N, Hayashi Y, Li C, Taneda S, Suzuki AK, Watanabe G, Taya K, Kamijima M, Nakajima T

    Toxicol Lett   Vol. 191 ( 2-3 ) page: 103-108   2009.8

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Books 1

  1. DEHP-induced reproductive and developmental toxicity and PPARα. In Biphenol A and Phthalates: Uses, Health Effects and Environmental Risks. DEHP-induced reproductive and developmental toxicity and PPARα

    Ito Y, Hayashi Y, Nakajima T.( Role: Joint author)

    Nova Science Publishers  2010 

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    Language:English

Presentations 32

  1. 非アルコール性脂肪性肝炎 (NASH) 発症・進展に食事性胆汁酸が与える影響

    北森一哉、木村藍、飛田博史、佐藤秀一、林由美、石川哲也、土倉覚、池田克巳、家森幸男、内藤久雄、那須民江

    第53回高血圧関連疾患モデル学会 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  2. PESI/MS/MSを用いたインタクト・メタボローム分析法の構築

    1. 林由美、 財津桂、 村田匡、大原倫美、近藤健太、石川哲也、土橋均、石井晃、緒方是嗣、谷畑博司

    第11回メタボローム・シンポジウム 

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    Event date: 2017.11

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  3. Rapid-fire direct metabolome analysis by probe electrospray ionization/tandem mass spectrometry: its preclinical applications to validate the methods. International conference

    Yumi Hayashi, Kei Zaitsu, Tasuku Murata, Tomomi Ohara, Kenta Kondo, Maiko Kusano, Koretsugu Ogata, Hitoshi Tsuchihashi, Hiroshi Tanihata, Akira Ishii, Tetsuya Ishikawa

    The 4th Annual European Congress of the Association for Mass Spectrometry: Application to the Clinical Lab(MSACL 2017 EU) 

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    Event date: 2017.9

    Language:English   Presentation type:Poster presentation  

    Country:Austria  

  4. Development of an in vivo real-time monitoring system using probe electrospray ionization/tandem mass spectrometry (PESI/MS/MS) and its preliminary applications International conference

    Kei Zaitsu, Yumi Hayashi, Tasuku Murata, Tomomi Ohara, Stéphane Moreau, Maiko Kusano, Hiroki Nakajima, Hiroshi Tanihata, Hioshi Tsuchihashi, Tetsuya Ishikawa, Akira Ishii

    65th Conference of American Society for Mass Spectrometry 

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    Event date: 2017.6

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  5. High-throughput Intact Metabolome Analysis of Mouse Biological Specimens by Probe Electrospray Ionization/Tandem Mass Spectrometry (PESI/MS/MS) International conference

    Yumi Hayashi, Kei Zaitsu, Tasuku Murata, Shiono Baba, Tomomi Ohara, Stéphane Moreau, Maiko Kusano, Hiroki Nakajima, Hiroshi Tanihata, Hitoshi Tsuchihashi, Akira Ishii, Tetsuya Ishikawa

    65th Conference of American Society for Mass Spectrometry 

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    Event date: 2017.6

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  6. メタボロミクスによる妊娠期DEHP曝露における胎児低生存率の解析

    3. 林由美、財津桂、名仁澤英里、平野文香、大原倫美、野田 沙樹、石井晃、伊藤由起、那須民江、石川哲也

    第87回日本衛生学会 

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  7. Direct drug analysis in serum by probe electrospray ionization/tandem mass spectrometry and its preliminary application to a real-time metabolism study International conference

    The 9th Annual European Congress of the Association for Mass Spectrometry: Application to the Clinical Lab(MSACL 2017 US) 

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    Event date: 2017.1

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  8. nano-flow injectionESI/Q-TOFMSによる迅速メタボローム分析法の検討

    瀧健太朗、財津桂、林由美、浅野友美、草野麻衣子、土橋均、石井晃

    第41回日本医用マススペクトル学会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:愛知県産業労働センター(ウインク愛知)   Country:Japan  

  9. Application of intact metabolome analysis by probe electrospray ionization (PESI)/MS/MS to local distribution analysis and in vivo real-time monitoring International conference

    HAYASHI Yumi, ZAITSU Kei, MURATA Tasuku, NAKAJIMA Hiroki, KUSANO Maiko, TSUCHIHASHI Hitoshi, ISHII Akira, ISHIKAWA Tetsuya.

    The 3rd Annual European Congress of the Association for Mass Spectrometry: Application to the Clinical Lab(MSACL 2016 EU) 

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    Event date: 2016.9

    Language:English   Presentation type:Poster presentation  

    Venue:Salzburg Congress Center, Salzburg, Austria   Country:Austria  

  10. Real-time monitoring of glutamic acid in mouse brain by direct coupling of microdialysis to tandem mass spectrometry with the newly developed on-line desalting system International conference

    ZAITSU Kei, HAYASHI Yumi, KANAYAMA Hiroaki, TAKAGI Ryosuke, NODA Saki, KUSANO Maiko, MINOHATA Toshikazu, AZUMA Jun-ichi, KANAYAMA Nobuhiro, ISHIKAWA Tetsuya, TSUCHIHASHI Hitoshi, ISHII Akira.

    The 3rd Annual European Congress of the Association for Mass Spectrometry: Application to the Clinical Lab(MSACL 2016 EU) 

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    Event date: 2016.9

    Language:English   Presentation type:Poster presentation  

    Venue:Salzburg Congress Center, Salzburg, Austria   Country:Austria  

  11. Intact metabolome analysis of mice biological tissues by probe electrospray ionization-tandem mass spectrometry (PESI-MS/MS) and its application to real-time analysis International conference

    HAYASHI Yumi, ZAITSU Kei, MURATA Tasuku, NAKAJIMA Hiroki, KUSANO Maiko, TSUCHIHASHI Hitoshi, ISHII Akira, ISHIKAWA Tetsuya.

    Royal Society of Chemistry Tokyo International Conference 2016  

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    Event date: 2016.9

    Language:English   Presentation type:Poster presentation  

    Venue:Makuhari Messe, Chiba   Country:Japan  

  12. 脂質メディエーターとメタボローム解析よるPPARαノックアウトマウスを用いたコカイン誘発性肝障害の解析

    4. 篠田諭、林由美、財津桂、酒井佑一郎、山中麻由美、那須民江、石川哲也、吉本高士、草野麻衣子、石井晃

    第43回 日本毒性学会 

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    Event date: 2016.6

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  13. Development of a Hydroxyl-Position Determining Method for theMetabolites of Naphthoylindole-type Synthetic Cannabinoids by GC/MS/MS. International conference

    KUSANO Maiko, ZAITSU Kei, YAMANAKA Mayumi, HISATSUNE Kazuaki, ASANO Tomomi, TAKI Kentaro, HAYASHI Yumi, TSUCHIHASHI Hitoshi, ISHII Akira.

    Society pf Forensic Toxicologists(SOFT) 2015 ANNUAL MEETING 

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    Event date: 2015.10

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  14. 探針エレクトロスプレー/タンデム質量分析(PESI/MS/MS)によるマウス肝臓内のメタボローム直接分析法(インタクト・メタボローム分析法)の構築

    林 由美、財津 桂、 村田 匡、大原倫美、中切健太、草野麻衣子、那須民江、中島宏樹、石川哲也、土橋 均、石井 晃

    第40回日本医用マススペクトル学会 

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    Event date: 2015.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:静岡県浜松市・アクトシティ浜松   Country:Japan  

  15. セロトニン症候群モデルラットの脳および血漿メタボローム解析

    財津 桂、林 由美、木村 友哉、纐纈 雄太、小崎 朋子、山中 麻友美、草野 麻衣子、土橋 均、石井 晃

    第42回日本毒性学会 

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    Event date: 2015.6 - 2015.7

    Language:Japanese   Presentation type:Poster presentation  

    Venue:石川県金沢市   Country:Japan  

  16. Intact Metabolome Analysis of Mice Liver by Probe Electrospray Ionization-Tandem Mass Spectrometry (PESI-MS/MS) International conference

    HAYASHI Yumi, ZAITSU Kei, MURATA Tasuku, NAKAJIMA Hiroki, NAKAJIMA Tamie, TSUCHIHASHI Hitoshi, ISHII Akira, ISHIKAWA Tetsuya.

    63rd American Society for Mass Spectrometry 

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    Event date: 2015.5 - 2015.6

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  17. 胎生期DEHP曝露がグルコースとレプチンに与える影響

    林由美、伊藤由起、那須民江

    第85回日本衛生学会 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:和歌山県和歌山市・和歌山県民文化会館   Country:Japan  

  18. フタル酸ジ-2-エチルヘキシル(DEHP)による精巣発達毒性にPPARαは関与しない

    伊藤 由起、 林 由美、 岡本 あずさ、 上島 通浩、 那須 民江

    第85回日本衛生学会 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:和歌山県和歌山市・和歌山県民文化会館   Country:Japan  

  19. 血圧が高脂肪食摂取による肝線維化進展に影響を与えるかもしれない

    内藤 久雄、賈 小芳、Yetti Husna、北森 一哉、林 由美、 加藤 昌志、 那須 民江

    第85回日本衛生学会 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:和歌山県和歌山市・和歌山県民文化会館   Country:Japan  

  20. 合成カンナビノイドMAM2201を投与したラットの血漿メタボローム解析

    服部南平、財津 桂、林 由美、中山 浩、鈴木 圭、高原里奈、草野麻衣子、土橋 均、石井 晃

    第39回日本医用マススペクトル学会年会 

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    Event date: 2014.10

    Language:Japanese   Presentation type:Poster presentation  

    Venue:千葉   Country:Japan  

  21. 合成カンナビノイドMAM2201投与ラットの大脳メタボローム解析

    鈴木 圭、財津 桂、林 由美、中山 浩、服部南平、高原里奈、草野麻衣子、土橋 均、石井 晃

    第39回日本医用マススペクトル学会年会 

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    Event date: 2014.10

    Language:Japanese   Presentation type:Poster presentation  

    Venue:千葉   Country:Japan  

  22. フタル酸ジ-2-エチルヘキシル曝露による胎生期・発達期毒性

    林 由美、伊藤 由起、那須 民江

    第41回日本毒性学会年会 

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    Event date: 2014.7

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:神戸   Country:Japan  

  23. 合成カンナビノイドMAM2201のラット尿中代謝物の分析

    久恒一晃、 財津 桂、中山 浩、林 由美、松本 智寛、石場  厚、肥田 宗政、土橋 均、石井 晃

    日本法中毒学会第33年会 

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    Event date: 2014.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  24. Exposure to DEHP in the pregnant mice may suppress the amount of food intakes of offspring in the development stage International conference

    Yumi Hayashi, Yuki Ito, Hisao Naito, Takaaki Kondo, Michihiro Kamijima, Tamie Nakajima

    The XIII International Congress of Toxicology (ICT 2013) 

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    Event date: 2013.6 - 2013.7

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  25. 胎生期フタル酸ジ-2-エチルヘキシル曝露による低栄養と生活習慣病および脳への影響

    林由美、伊藤由起、近藤高明、上島通浩、那須民江

    日本DOHaD研究会 

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    Event date: 2013.6

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京都新宿区   Country:Japan  

  26. 胎生期フタル酸ジ-2-エチルヘキシル曝露がレプチンに及ぼす影響

    林由美、伊藤由起、内藤久雄、近藤高明、上島通浩、那須民江

    環境ホルモン学会 

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    Event date: 2012.12

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京都文京区   Country:Japan  

  27. 妊娠期DEHP曝露による母獣の血漿中トリグリセライド減少メカニズム

    林由美、山岸希、伊藤由起、内藤久雄、那須民江

    日本衛生学会 

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    Event date: 2011.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  28. 妊娠マウスのDEHP曝露はPPARαを介して胎仔・新生仔の生存に影響を与える

    林由美、伊藤由起、王棟、柳場由絵、山岸希、玉田葉月、ラムダンドニヒクマット、内藤久雄、那須民江

    産業衛生学会 

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    Event date: 2010.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  29. Di (2-ethyhexy) phthalate may cause reproductive and developmental toxicity through PPARα International conference

    Hayashi Y,Ito Y,Nakajima T.

    International Congress of Toxicology 

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    Event date: 2010.7

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  30. 胎生期DEHP曝露による低栄養と生活習慣病リスクとの関連

    林由美、伊藤由起、柳場由絵、王棟、山岸希、賈小芳、内藤久雄、那須民江

    日本衛生学会 

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    Event date: 2010.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  31. 核内受容体PPARαによるフタル酸ジ-2-エチルヘキシルの生殖・次世代影響の制御

    林由美、伊藤由起、玉田葉月、山岸希、柳場由絵、王棟、上島通浩、那須民江

    日本衛生学会 

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    Event date: 2009.3 - 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  32. ディーゼル排気ナノ粒子曝露の肝臓への影響とそのメカニズム

    林由美、伊藤由起、ラムダンドニヒクマット、堀心一、李宇飛、柳場由絵、李春梅、種田晋二、鈴木明、上島通浩、那須民江

    日本衛生学会 

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    Event date: 2008.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

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KAKENHI (Grants-in-Aid for Scientific Research) 10

  1. Establishment of a liver regeneration treatment model based on cell transplantation and development of a new treatment against immune rejection.

    Grant number:21K07959  2021.4 - 2024.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

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    Authorship:Coinvestigator(s) 

  2. 新規質量分析PESI/MS/MSによる簡便・迅速な作物のメタボローム解析法の開発

    Grant number:20K20372  2020.4 - 2022.3

    科学研究費助成事業  挑戦的研究(開拓)

    白武 勝裕, 財津 桂, 林 由美

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    Authorship:Coinvestigator(s) 

    本研究は,生物の代謝物を,生きたまま,非破壊で,抽出や前処理などの操作一切無しに,1検体わずか数秒で解析できる新しい質量分析装置「探針エレクトロスプレー/タンデム質量分析装置(PESI/MS/MS)」を,植物のメタボローム解析(代謝物の一斉解析)に初めて導入し,植物学研究,農作物研究,農業関連産業において有効な分析装置であることを示し,その実証例を提示することを目的としている.
    [1,植物の組織・器官の直接解析],[2,植物からの抽出物の解析],[3,植物の代謝のリアルタイム・モニタリング],[4,植物の細胞・組織・器官の代謝物イメージング],[5,植物ホルモンの定量],[6,農作物の残留農薬の分析]の6つの分析法の確立とその実証例の提示を目標に掲げた.3年目(2020年度)は,2年目(2019年度)から継続の3および5と6の実施を計画したが,新型コロナウイルスの影響を受け,3と5については計画した一部の実験の実施に留まり,6については実施できなかった.
    3,植物の代謝のリアルタイム・モニタリング:当初計画した,光のON-OFFにより動く光合成関連一次代謝経路(カルビン回路)の代謝物の測定を試みたが,上手く代謝物変化を捉えることができなかった.これは,代謝反応が速く(瞬間的な代謝物の蓄積量が少なく)上手く測定できないことが原因だと考えられる.そのため,4年目(2021年度)は,病原菌の感染により変化する二次代謝物のリアルタイム・モニタリングを試みる.
    5,植物ホルモンの定量:2年目(2019年度)にPESI/MS/MSで検出できることが確認できたGA3について,GA3標品を用いて検量線の作成を試み,検量線の作成に成功した.しかしながら,実試料(イチゴの葉)を用いた測定でGA3を検出ができなかった.これは,試料に含まれる夾雑成分のマトリクス効果あるいはGA3の濃度の低さによるものと考えられる.今後 GA3含量が多い植物試料における測定を実施すると共に,他の植物ホルモンの分析を実施する.
    また,PESI/MS/MS による質量分析からデータ解析までを全自動で一気貫通で行うプラットフォームPiTMaPを開発した.このPiTMaPは動物の代謝物測定用に開発したものであるが,植物の代謝物測定においても質量分析からデータ解析までを全自動することは重要であり,今後PiTMaPを植物の代謝物測定にも応用して行く.
    当初計画では,[1,植物の組織・器官の直接解析],[2,植物からの抽出物の解析],[3,植物の代謝のリアルタイム・モニタリング],[4,植物の細胞・組織・器官の代謝物イメージング],[5,植物ホルモンの定量],[6,農作物の残留農薬の分析]の6つの分析法の確立とその実証例の提示を目標に掲げた.1年目(2018年度)は当初計画通り1と2を実施し,完了した.2年目(2019年度)は,当初計画では3と4を実施予定であったが,これを変更して4と5を実施し,3年目(2020年度)に3と6を実施するように,計画を変更した.
    2年目(2019年度)に[4,植物の細胞・組織・器官の代謝物イメージング]は完了することができたが,[5,植物ホルモンの定量]については実験が遅れ,3年目(2020年度)に継続実施することとした.3年目(2020年度)は,新型コロナウイルスの影響を大きく受け,[3,植物の代謝のリアルタイム・モニタリング]と[5,植物ホルモンの定量]については一部の実験を実施するに留まり,[6,農作物の残留農薬の分析]については実施ができなかったため,研究期間を延長し,4年目(2021年度)に実施することとした.現在,新型コロナウイルスの影響を受けながらも,通常レベルに近い実験の実施可能となっており,残った実験を2021年度中に完了したい.
    当初計画では,[1,植物の組織・器官の直接解析],[2,植物からの抽出物の解析],[3,植物の代謝のリアルタイム・モニタリング],[4,植物の細胞・組織・器官の代謝物イメージング],[5,植物ホルモンの定量],[6,農作物の残留農薬の分析]の6つの分析法の確立とその実証例の提示を目標に掲げた.1年目(2018年度)と2年目(2019年度)において1,2,4を完了し,3年目(2020年度)に3,5,6を実施する予定であったが,新型コロナウイルスの影響を大きく受け実験を完了できなかったたため,研究期間を延長し,4年目(2021年度)に実施する.
    [3,植物の代謝のリアルタイム・モニタリング]については,光合成関連一次代謝経路の代謝物の測定を試みたが,代謝反応が速く(瞬間的な代謝物の蓄積量が少なく)上手く測定できない可能性が考えられたため,4年目(2021年度)は,病原菌の感染により変化する二次代謝物のリアルタイム・モニタリングを試みる.[5,植物ホルモンの定量]については,GA3標品を用いた検量線の作成に成功したが,実試料(イチゴの葉)を用いた測定でGA3を検出ができなかった.これは,試料に含まれる夾雑成分のマトリクス効果あるいはGA3の濃度の低さによるものと考えられ,GA3含量が多い試料を用いた測定を実施する.また,ジベレリン類以外の植物ホルモン(オーキシン類,サイトカイニン類,アブシジン酸,ブラシノステロイド類,ジャスモン酸類,サリチル酸,ストリゴラクトン類など)についても測定を試みる.

  3. 肝臓X受容体を介した非アルコール性脂肪性肝炎と心臓血管疾患をつなぐ臓器連関の解明

    Grant number:18K10993  2018.4 - 2022.3

    科学研究費助成事業  基盤研究(C)

    渡辺 彰吾, 林 由美

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    Authorship:Coinvestigator(s) 

    非アルコール性脂肪性肝炎(NASH)では、肝臓が担う脂質や糖などの栄養代謝の異常を介して、動脈硬化を悪化させ、心臓や血管にまで重篤な悪影響を及ぼす。本研究では、肝臓疾患であるNASHと心臓・血管疾患をつなぐ「臓器連関」を解明し、効果的な先制的治療法を見出すため、肝臓や血管などに発現している肝臓X受 容体(LXR)に着目した。このLXR活性は一度形成されたアテローム性脂肪沈着巣からコレステロールを引き抜き、動脈硬化を退縮させる革新的な効果が期待されて いるが、脂質合成系遺伝子(SREBP1c)も誘導して、肝臓の脂質沈着をさらに悪化させる欠点がある。本研究では、この欠点を克服するため、目的1:胆汁酸による SREBP1c活性の抑制効果と目的2:SREBP1cを活性させないLXR活性の新規リガンド候補であるウアバゲニンの効用について、申請者らが所有する特殊なNASH-心筋 梗塞発症モデル(SHRSP5/Dmcrラット)を使用して検討する。2018年度は、本研究で使用しているSHRSP5ラットの胆汁酸基礎代謝について調べ、血中および肝臓中 の胆汁酸上昇にともなって、肝線維化、動脈の脂質沈着、心筋梗塞が悪化することを証明し、Int J Exp pathに掲載された。2019年度は、予定通り、東北大学よ りウアバゲニンの提供を受け、NASHにおける肝臓線維化、動脈における脂質沈着が改善された。2020年度では、さらなる条件検討、ウアバゲニンの構造類似体であるジゴキシゲニン、ジギトキシゲニンなどの投与を行い、ウアバゲニンの有用性を確認できた。本結果は現在、海外ジャーナルへ投稿予定である。
    当初の研究仮説と概ね同様の実験結果が得られ、研究計画を変更する必要はないため。
    当初の予定を早め、LXRの新規リガンドの候補であるウアバゲニンを投与し、そのリガンド活性がSREBP1cを活性化させず、動脈硬化や心筋梗塞を改善し、かつ肝臓の脂肪蓄積を悪化させないことを確認できたが、すでに形成されたアテローム動脈硬化巣を退縮させるか否かは、詳細な検討が必要であった。昨年度は実際に重篤な動脈硬化を起こした状態でのウアバゲニン投与を試みて、脂質沈着の退縮が認められた。今年度はこれまでの研究成果を海外ジャーナルに投稿し、追加実験に備える。

  4. Accurate measurement of natural toxins in body fluids using deuterated standards and search of specific markers for the rapid diagnosis

    Grant number:18H03064  2018.4 - 2021.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    Ishii Akira

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    Authorship:Coinvestigator(s) 

    The purpose of the present study is to develop methods for sensitive identification and precise quantification of natural toxins in human biological specimens using deuterium labeled natural toxins by deuterium exchange reactions. Although we have not obtained deuterium labeled natural toxins, we have developed two methods for screening, identifying and quantification natural toxins in human biological specimens: a screening system for 56 natural toxins by liquid chromatography-quadrupole time-of-flight tandem mass spectrometry (LC-QTOFMS), and a sensitive quantification method for 12 plant toxins by LC-tandem mass spectrometry, combined with simple solid-phase extraction.

  5. Elucidation of acute toxicity of drugs by real-time metabolome analysis and multivariate time-series analysis

    Grant number:18K10122  2018.4 - 2021.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Zaitsu Kei

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    Authorship:Coinvestigator(s) 

    In this study, our research team have developed a real-time metabolome analytical technique using probe electrospray ionization tandem mass spectrometry (PESI/MS/MS) with time-series multivariate analyses. We optimized analytical conditions for real-time mass spectrometry, achieving successfully monitoring of 40 metabolites in a living mouse. Our research team has also developed a data pipeline using the R software, and this data pipeline can automatically execute bioinformatics analyses such as multivariate analysis. Moreover, we have developed a new approach using a state space model based on Bayesian statistical modeling for time-series data obtained from real-time monitoring. Finally, we applied this technique to a living mouse, demonstrating the practicality of our technique.

  6. Investigation of pathogenesis of infant hypothermia-the association between undernutrition during gestation and thermogenesis-

    Grant number:16K19271  2016.4 - 2018.3

    HAYASHI Yumi

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    Authorship:Principal investigator 

    Grant amount:\4030000 ( Direct Cost: \3100000 、 Indirect Cost:\930000 )

    In this study, we attempted to develop metabolome analysis techniques and create a model mouse of hypothermia to observe body temperature, activity and energy metabolism in infant exposed to undernutrition during gestation. Metabolome analysis methods in liver, muscle and brain were developed using GC/MS/MS and probe electrospray ionization/tandem mass spectrometry (PESI/MS/MS). We tried to create a model mouse of hypothermia induced by food restriction. Mice exposed to food restriction for 10 days or more did not give birth. In 5 days restriction, mice could give birth, but body temperature of the offspring in weaning period was almost same in control group. Therefore, we continue to consider creation of a model mouse of hypothermia.

  7. Application of novel mass spectrometric techniques for highly sensitive, specific and speedy identification of new psychoactive substances in human specimens

    Grant number:15H02530  2015.4 - 2018.3

    Ishii Akira

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    Authorship:Coinvestigator(s) 

    The aim of this project is to develop simple and comprehensive methods for determining novel abused drugs including synthetic cannabinoids by combining ultra-sensitive and/or highly specific mass spectrometers, and conventional mass spectrometers. We developed a simple method for determining several synthetic cannabinoids and their metabolites using a high-performance liquid chromatography-quadrupole time-of-flight mass spectrometer (LC-QTOFMS). The combination of an LC-QTOFMS and an LC-QTrap enabled the first identification of 5-fluoro-ADB (5-F-ADB) in the blood of a fatal intoxication case of 5-F-ADB and diphenidine. We then proposed possible metabolic pathways of the compounds. We are now investigating the difference of 5-F-ADB levels among organs in which the expression levels of carboxyesterase differ.

  8. 胎生期DEHP曝露による慢性炎症を基盤とした生活習慣病進展メカニズム

    2014.4 - 2016.3

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

  9. 胎生期DEHP曝露による仔の栄養状態と海馬・視床下部に及ぼす影響

    2012.10 - 2014.3

    科学研究費補助金 

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    Authorship:Principal investigator 

  10. DEHP胎生期曝露に招来される低栄養を特徴づける遺伝子変化と生活習慣病リスク

    2010.4 - 2012.3

    科学研究費補助金 

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    Authorship:Principal investigator 

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Industrial property rights 1

  1. 質量分析装置及び該装置を用いた生体試料の分析方法

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    Application no:201680083219.X  Date applied:2016.3

    Patent/Registration no:ZL201680083219.X  Date registered:2021.2