Updated on 2026/04/30
Doctor of Philosophy (Medical Science) （ 2011.11 Nagoya University ）
DISC1
Signal transduction
dopamine
schizophrenia
Cell Biology
Psychiatry
Biochemistry
包括脳ネットワーク
Small GTPase
Life Science / Pharmacology
Life Science / Cell biology
Life Science / Psychiatry
Life Science / Neuroscience - general
Life Science / Molecular biology
Nagoya University School of Medicine Department of Medicine
2026.4
Nagoya University Graduate School of Medicine
2026.4
Nagoya University Graduate School of Medicine Program in Integrated Medicine Associate Professor
2025.4
Nagoya University Graduate School of Medicine Designated Associate Professor
2021.4 - 2025.3
Nagoya University Graduate School of Medicine Designated Assistant Professor
2012.1 - 2021.3
Nagoya University Graduate School of Medicine guest researcher
2010.4 - 2011.12
Nagoya University Graduate School, Division of Medical Sciences
2006.4 - 2010.3
Country： Japan
Nagoya University Graduate School of Medicine
2006.4 - 2010.3
Nagoya University Faculty of Medicine
1998.4 - 2004.3
Country： Japan
Nagoya University School of Medicine
1998.4 - 2004.3
Society for Neuroscience
THE JAPANESE SOCIETY FOR NEUROCHEMISTRY
THE JAPANESE PHARMACOLOGICAL SOCIETY
THE JAPAN NEUROSCIENCE SOCIETY
Balance between dopamine and adenosine signals regulates the PKA/Rap1 pathway in striatal medium spiny neurons. Reviewed International journal Open Access
Xinjian Zhang, Taku Nagai, Rijwan Uddin Ahammad, Keisuke Kuroda, Shinichi Nakamuta, Takashi Nakano, Naoto Yukinawa, Yasuhiro Funahashi, Yukie Yamahashi, Mutsuki Amano, Junichiro Yoshimoto, Kiyofumi Yamada, Kozo Kaibuchi
Neurochemistry international Vol. 122 page： 8 - 18 2019.1
Keisuke Kuroda, Shinnosuke Yamada, Motoki Tanaka, Michiro Iizuka, Hisashi Yano, Daisuke Mori, Daisuke Tsuboi, Tomoki Nishioka, Takashi Namba, Yukihiko Iizuka, Shimpei Kubota, Taku Nagai, Daisuke Ibi, Rui Wang, Atsushi Enomoto, Mayu Isotani-Sakakibara, Naoya Asai, Kazushi Kimura, Hiroshi Kiyonari, Takaya Abe, Akira Mizoguchi, Masahiro Sokabe, Masahide Takahashi, Kiyofumi Yamada, Kozo Kaibuchi
HUMAN MOLECULAR GENETICS Vol. 20 ( 23 ) page： 4666 - 4683 2011.12
Signal flow in the NMDA receptor-dependent phosphoproteome regulates postsynaptic plasticity for aversive learning. International journal Open Access
Yasuhiro Funahashi, Rijwan Uddin Ahammad, Xinjian Zhang, Emran Hossen, Masahiro Kawatani, Shinichi Nakamuta, Akira Yoshimi, Minhua Wu, Huanhuan Wang, Mengya Wu, Xu Li, Md Omar Faruk, Md Hasanuzzaman Shohag, You-Hsin Lin, Daisuke Tsuboi, Tomoki Nishioka, Keisuke Kuroda, Mutsuki Amano, Yukihiko Noda, Kiyofumi Yamada, Kenji Sakimura, Taku Nagai, Takayuki Yamashita, Shigeo Uchino, Kozo Kaibuchi
Science signaling Vol. 17 ( 853 ) page： eado9852 2024.9
Dopamine drives neuronal excitability via KCNQ channel phosphorylation for reward behavior. Reviewed International journal Open Access
Daisuke Tsuboi, Takeshi Otsuka, Takushi Shimomura, Md Omar Faruk, Yukie Yamahashi, Mutsuki Amano, Yasuhiro Funahashi, Keisuke Kuroda, Tomoki Nishioka, Kenta Kobayashi, Hiromi Sano, Taku Nagai, Kiyofumi Yamada, Anastasios V Tzingounis, Atsushi Nambu, Yoshihiro Kubo, Yasuo Kawaguchi, Kozo Kaibuchi
Cell reports Vol. 40 ( 10 ) page： 111309 - 111309 2022.9
NMDA-induced activation of the CaMKII-RhoA-Rho-kinase pathway regulates aversive learning
Funahashi Yasuhiro, Ahammad Rijwan Uddin, Zhang Xinjian, Emran Hossen, Faruk Md. Omar, Wang Huanhuan, Wu Minhua, Xu Yifan, Tsuboi Daisuke, Nishioka Tomoki, Kuroda Keisuke, Amano Mutsuki, Sakimura Kenji, Uchino Shigeo, Yamada Kiyofumi, Nagai Taku, Kaibuchi Kozo
Proceedings for Annual Meeting of The Japanese Pharmacological Society Vol. 96 page： 2-B-P-109 2022
Accumbal D2R-medium spiny neurons regulate aversive behaviors through PKA-Rap1 pathway Reviewed International journal Open Access
You-Hsin Lin, Yukie Yamahashi, Keisuke Kuroda, Md. Omar Faruk, Xinjian Zhang, Kiyofumi Yamada, Akihiro Yamanaka, Taku Nagai, Kozo Kaibuchi
Neurochemistry International Vol. 143 page： 104935 - 104935 2021.2
Nagaoka, K., Nagashima, T., Asaoka, N., Yamamoto, H., Toda, C., Kayanuma, G., Siswanto, S., Funahashi, Y., Kuroda, K., Kaibuchi, K., Mori, Y., Nagayasu, K., Shirakawa, H., Kaneko, S.
JCI Insight Vol. 6 ( 10 ) 2021
ARHGAP10, which encodes Rho GTPase-activating protein 10, is a novel gene for schizophrenia risk Reviewed International journal Open Access
Sekiguchi, M., Sobue, A., Kushima, I., Wang, C., Arioka, Y., Kato, H., Kodama, A., Kubo, H., Ito, N., Sawahata, M., Hada, K., Ikeda, R., Shinno, M., Mizukoshi, C., Tsujimura, K., Yoshimi, A., Ishizuka, K., Takasaki, Y., Kimura, H., Xing, J., Yu, Y., Yamamoto, M., Okada, T., Shishido, E., Inada, T., Nakatochi, M., Takano, T., Kuroda, K., Amano, M., Aleksic, B., Yamomoto, T., Sakuma, T., Aida, T., Tanaka, K., Hashimoto, R., Arai, M., Ikeda, M., Iwata, N., Shimamura, T., Nagai, T., Nabeshima, T., Kaibuchi, K., Yamada, K., Mori, D., Ozaki, N.
Translational Psychiatry Vol. 10 ( 1 ) page： 247 2020
Phosphorylation of Npas4 by MAPK Regulates Reward-Related Gene Expression and Behaviors. Reviewed International journal Open Access
Yasuhiro Funahashi, Anthony Ariza, Ryosuke Emi, Yifan Xu, Wei Shan, Ko Suzuki, Sachi Kozawa, Rijwan Uddin Ahammad, Mengya Wu, Tetsuya Takano, Yoshimitsu Yura, Keisuke Kuroda, Taku Nagai, Mutsuki Amano, Kiyofumi Yamada, Kozo Kaibuchi
Cell reports Vol. 29 ( 10 ) page： 3235 - 3252 2019.12
Comprehensive analysis of kinase-oriented phospho-signalling pathways. Reviewed International journal Open Access
Mutsuki Amano, Tomoki Nishioka, Daisuke Tsuboi, Keisuke Kuroda, Yasuhiro Funahashi, Yukie Yamahashi, Kozo Kaibuchi
Journal of biochemistry Vol. 165 ( 4 ) page： 301 - 307 2019.4
KANPHOS (Kinase-Associated Phospho-Signaling) Platform - A comprehensive database for kinase-associated neural phosphorylation signaling
Kuroda Keisuke, Nagai Taku, Amano Mutsuki, Yoshimoto Junichiro, Kannon Takayuki, Nishioka Tomoki, Usui Shiro, Kaibuchi Kozo
Proceedings for Annual Meeting of The Japanese Pharmacological Society Vol. 92 ( 0 ) page： 1 - P-132 2019
Cell type-specific activation of mitogen-activated protein kinase in D1 receptor-expressing neurons of the nucleus accumbens potentiates stimulus-reward learning in mice Reviewed International journal Open Access
Bin Saifullah, Md. Ali, Nagai Taku, Kuroda Keisuke, Wulaer Bolati, Nabeshima Toshitaka, Kaibuchi Kozo, Yamada Kiyofumi
Scientific Reports Vol. 8 ( 1 ) page： 14413 - 14413 2018.12
Repetitive and compulsive-like behaviors lead to cognitive dysfunction in Disc1<sup>Δ2-3/Δ2-3</sup> mice Reviewed Open Access
Wulaer, B., Nagai, T., Sobue, A., Itoh, N., Kuroda, K., Kaibuchi, K., Nabeshima, T., Yamada, K.
Genes, Brain and Behavior Vol. 17 ( 8 ) 2018.11
IN VIVO IMAGING FOR THE EFFECT OF COCAINE
Zhu Y., Ito N., Nagai T., Kuroda K., Kaibuchi K., Nabeshima T., Yamada K.
ALCOHOLISM-CLINICAL AND EXPERIMENTAL RESEARCH Vol. 42 page： 28A-28A 2018.8
Dopamine-induced phosphorylation of NPAS4 through MAPK regulates reward-related learning and memory
Funahashi Y., Ariza A., Suzuki K., Wei S., Kozawa S., Takano T., Kuroda K., Nagai T., Kaibuchi K.
JOURNAL OF NEUROCHEMISTRY Vol. 142 page： 129-129 2017.8
Phosphorylation signals in hippocampal and striatal synapses
Kaibuchi K., Kuroda K., Yoshikawa M.
JOURNAL OF NEUROCHEMISTRY Vol. 142 page： 22-22 2017.8
KANPHOS(Kinase-Associated Phospho-Signaling)Platform 新規リン酸化DBプラットフォーム
天野 睦紀, 西岡 朋生, 黒田 啓介, 吉本 潤一郎, 観音 隆幸, 臼井 支朗, 貝淵 弘三
日本細胞生物学会大会講演要旨集 Vol. 69回 page： 83 - 83 2017.5
Neuropeptide Y neuronal network dysfunction in the frontal lobe of a genetic mouse model of schizophrenia Reviewed Open Access
Shunsuke Morosawa, Shuji Iritani, Hiroshige Fujishiro, Hirotaka Sekiguchi, Youta Torii, Chikako Habuchi, Keisuke Kuroda, Kozo Kaibuchi, Norio Ozaki
NEUROPEPTIDES Vol. 62 page： 27 - 35 2017.4
RhoA activity regulates spine morphology and memory formation
Nakanose Yuki, Kuroda Keisuke, Zhang Xinjian, Oda Kaishu, Nagai Taku, Kaibuchi Kozo
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 133 ( 3 ) page： S160-S160 2017.3
KANPHOS (Kinase-Associated Phospho-Signaling) Platform - A database for neural phosphoproteomics with quality control
Kuroda Keisuke, Nagai Taku, Amano Mutsuki, Yoshimoto Junichiro, Kannon Takayuki, Nishioka Tomoki, Usui Shiro, Kaibuchi Kozo
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 133 ( 3 ) page： S263-S263 2017.3
Dopamine-induced phosphorylation of NPAS4 through MAPK regulates reward-related learning and memory
Yasuhiro Funahashi, Ariza Anthony, Wei Shan, Kozawa Sachi, Okuda Keiichiro, Suzuki Ko, Takano Tetsuya, Yura Yoshimitsu, Kuroda Keisuke, Nagai Taku, Kaibuchi Kozo
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 133 ( 3 ) page： S216-S216 2017.3
Elevated repetitive and compulsive-like behavior in a schizophrenia animal model
Wulaer Bolati, Nagai Taku, Sobue Akira, Kuroda Keisuke, Kaibuchi Kozo, Nabeshima Toshitaka, Yamada Kiyotumi
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 133 ( 3 ) page： S219-S219 2017.3
Cell-type-specific isolation of 14-3-3 associated phosphoprotein from complex brain tissues
Yoshikawa Marina, Kuroda Keisuke, Nagai Taku, Kaibuchi Kozo
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 133 ( 3 ) page： S159-S159 2017.3
The activity-dependent transcription factor NPAS4 regulates reward-related learning and memory
Suzuki Ko, Funahashi Yasuhiro, Ariza Anthony, Wei Shan, Kuroda Keisuke, Nagai Taku, Kaibuchi Kozo
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 133 ( 3 ) page： S164-S164 2017.3
Cell-type-specific isolation of 14-3-3 associated phosphoprotein from complex brain tissues.
Yoshikawa M., Kuroda K., Nagai T., Kaibuchi K.
MOLECULAR BIOLOGY OF THE CELL Vol. 28 page： . 2017
RhoA activity regulates spine morphology and memory associated behavior.
Kuroda K., Zhang X., Oda K., Nakanose Y., Yoshikawa M., Nagai T., Kaibuchi K.
MOLECULAR BIOLOGY OF THE CELL Vol. 28 page： . 2017
Kentaro Umeda, Shuji Iritani, Hiroshige Fujishiro, Hirotaka Sekiguchi, Youta Torii, Chikako Habuchi, Keisuke Kuroda, Kozo Kaibuchi, Norio Ozaki
SYNAPSE Vol. 70 ( 12 ) page： 508 - 518 2016.12
Phosphorylation Signals in Striatal Medium Spiny Neurons Reviewed
Taku Nagai, Junichiro Yoshimoto, Takayuki Kannon, Keisuke Kuroda, Kozo Kaibuchi
TRENDS IN PHARMACOLOGICAL SCIENCES Vol. 37 ( 10 ) page： 858 - 871 2016.10
Survival of corticostriatal neurons by Rho/Rho-kinase signaling pathway Reviewed
Kenta Kobayashi, Hiromi Sano, Shigeki Kato, Keisuke Kuroda, Shinichi Nakamuta, Tadashi Isa, Atsushi Nambu, Kozo Kaibuchi, Kazuto Kobayashi
NEUROSCIENCE LETTERS Vol. 630 page： 45 - 52 2016.9
Shuji Iritani, Hirotaka Sekiguchi, Chikako Habuchi, Youta Torii, Keisuke Kuroda, Kozo Kaibuchi, Norio Ozaki
ACTA NEUROPSYCHIATRICA Vol. 28 ( 2 ) page： 117 - 123 2016.4
Dopamine D1 receptor stimulation leads to Rap1 activation as a reward signal in vivo. Reviewed Open Access
Nagai T, Nakamuta S, Kuroda K, Nakauchi S, Nishioka T, Takano T, Xinjian Zhang, Kenta Kobayashi K, Miura M, Nishi A, Kobayashi K, Yamada K, Amano M, Kaibuchi K
Neuron Vol. 89 ( 3 ) page： 550 - 565 2016.2
Focused Proteomics Revealed a Novel Rho-kinase Signaling Pathway in the Heart Reviewed Open Access
Yoshimitsu Yura, Mutsuki Amano, Mikito Takefuji, Tomohiro Bando, Kou Suzuki, Katsuhiro Kato, Tomonari Hamaguchi, Md. Hasanuzzaman Shohag, Tetsuya Takano, Yasuhiro Funahashi, Shinichi Nakamuta, Keisuke Kuroda, Tomoki Nishioka, Toyoaki Murohara, Kozo Kaibuchi
CELL STRUCTURE AND FUNCTION Vol. 41 ( 2 ) page： 105 - 120 2016
Stimulation of synaptic vesicle exocytosis by the mental disease gene DISC1 is mediated by N-type voltage-gated calcium channels Reviewed Open Access
Willcyn Tang, Jervis Vermal Thevathasan, Qingshu Lin, Kim Buay Lim, Keisuke Kuroda, Kozo Kaibuchi, Marcel Bilger, Tuck Wah Soong, Marc Fivaz
Frontiers in Synaptic Neuroscience Vol. 8 page： 15 2016
Impairment of molecular transport in a mouse model of Schizophrenia Reviewed
D. Tsuboi, K. Kuroda, M. Tanaka, T. Namba, S. Taya, N. Ozaki, K. Kaibuchi
JOURNAL OF NEUROCHEMISTRY Vol. 134 page： 356 - 356 2015.8
Potential involvement of kinesin-1 in the regulation of subcellular localization of Girdin Reviewed Open Access
Aya Muramatsu, Atsushi Enomoto, Takuya Kato, Liang Weng, Keisuke Kuroda, Naoya Asai, Masato Asai, Shinji Mu, Masahide Takahashi
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Vol. 463 ( 4 ) page： 999 - 1005 2015.8
Disrupted-in-schizophrenia 1 regulates transport of ITPR1 mRNA for synaptic plasticity Reviewed
Daisuke Tsuboi, Keisuke Kuroda, Motoki Tanaka, Takashi Namba, Yukihiko Iizuka, Shinichiro Taya, Tomoyasu Shinoda, Takao Hikita, Shinsuke Muraoka, Michiro Iizuka, Ai Nimura, Akira Mizoguchi, Nobuyuki Shiina, Masahiro Sokabe, Hideyuki Okano, Katsuhiko Mikoshiba, Kozo Kaibuchi
NATURE NEUROSCIENCE Vol. 18 ( 5 ) page： 698 - + 2015.5
Disrupted-in-schizophrenia-1 (DISC1) Regulates Endoplasmic Reticulum Calcium Dynamics Reviewed Open Access
Sung Jin Park, Jaehoon Jeong, Young-Un Park, Kyung-Sun Park, Haeryun Lee, Namgyu Lee, Sung-Mo Kim, Keisuke Kuroda, Minh Dang Nguyen, Kozo Kaibuchi, Sang Ki Park
SCIENTIFIC REPORTS Vol. 5 2015.3
Regulation of cargo-selective endocytosis by dynamin 2 GTPase-activating protein girdin Reviewed Open Access
Liang Weng, Atsushi Enomoto, Hiroshi Miyoshi, Kiyofumi Takahashi, Naoya Asai, Nobuhiro Morone, Ping Jiang, Jian An, Takuya Kato, Keisuke Kuroda, Takashi Watanabe, Masato Asai, Maki Ishida-Takagishi, Yoshiki Murakumo, Hideki Nakashima, Kozo Kaibuchi, Masahide Takahashi
EMBO JOURNAL Vol. 33 ( 18 ) page： 2098 - 2112 2014.9
Tsuyoshi Nakai, Taku Nagai, Rui Wang, Shinnosuke Yamada, Keisuke Kuroda, Kozo Kaibuchi, Kiyofumi Yamada
NEUROCHEMISTRY INTERNATIONAL Vol. 74 page： 74 - 83 2014.7
Neuronal Per Arnt Sim (PAS) Domain Protein 4 (NPAS4) Regulates Neurite Outgrowth and Phosphorylation of Synapsin I Reviewed Open Access
Jaesuk Yun, Taku Nagai, Yoko Furukawa-Hibi, Keisuke Kuroda, Kozo Kaibuchi, Michael E. Greenberg, Kiyofumi Yamada
JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 288 ( 4 ) page： 2655 - 2664 2013.1
Analysis of GABA system in DISC1 homozygous mutant mice Reviewed
Nakai, T., Nagai, T., Yamada, S., Nakajima, A., Kuroda, K., Enomoto, A., Kaibuchi, K., Yamada, K.
Society for Neuroscience Abstract Viewer and Itinerary Planner Vol. 42 2012
Disrupted-In-Schizoprenia-1 regulates transport of neuronal mRNA Reviewed
Tsuboi, D., Kuroda, K., Iizuka, Y., Okano, H., Mikoshiba, K., Kaibuchi, K.
Society for Neuroscience Abstract Viewer and Itinerary Planner Vol. 42 2012
Behavioral alterations associated with targeted disruption of the Disc1 gene in the mouse Reviewed
Kuroda, K., Yamada, S., Tanaka, M., Sokabe, M., Yamada, K., Kaibuchi, K.
Society for Neuroscience Abstract Viewer and Itinerary Planner Vol. 42 2012
Girdin Is an Intrinsic Regulator of Neuroblast Chain Migration in the Rostral Migratory Stream of the Postnatal Brain Reviewed Open Access
Yun Wang, Naoko Kaneko, Naoya Asai, Atsushi Enomoto, Mayu Isotani-Sakakibara, Takuya Kato, Masato Asai, Yoshiki Murakumo, Haruko Ota, Takao Hikita, Takashi Namba, Keisuke Kuroda, Kozo Kaibuchi, Guo-li Ming, Hongjun Song, Kazunobu Sawamoto, Masahide Takahashi
JOURNAL OF NEUROSCIENCE Vol. 31 ( 22 ) page： 8109 - 8122 2011.6
Hirotaka Sekiguchi, Shuji Iritani, Chikako Habuchi, Yohta Torii, Keisuke Kuroda, Kozo Kaibuchi, Norio Ozaki
BRAIN RESEARCH Vol. 1392 page： 47 - 53 2011.5
Behavioral analysis of DISC1 knockout mice Reviewed
Shinnosuke Yamada, Daisuke Ibi, Keisuke Kuroda, Rui Wang, Taku Nagai, Kozo Kaibuchi, Kiyofumi Yamada
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 115 page： 194P - 194P 2011
Proteomic analysis reveals novel binging partner of DISC1, a candidate risk gene of schizophrenia Reviewed
Yukihiko Iizuka, Takafumi Kinoshita, Daisuke Tsuboi, Daisuke Mori, Keisuke Kuroda, Kozo Kaibuchi
NEUROSCIENCE RESEARCH Vol. 71 page： E300 - E300 2011
Npas4 regulates neurite outgrowth and phosphoryation of synapsin I Reviewed
Jaesuk Yun, Taku Nagai, Yoko Hibi, Keisuke Kuroda, Kozo Kaibuchi, Kiyofumi Yamada
NEUROSCIENCE RESEARCH Vol. 71 page： E235 - E235 2011
Npas4 regulates neurite development and phosphoryation of synapsin I Reviewed
Jaesuk Yun, Taku Nagai, Yoko Hibi, Keisuke Kuroda, Kozo Kaibuchi, Kiyofumi Yamada
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 115 page： 132P - 132P 2011
Histological investigation in the brain of genetically modified mouse of schizophrenia Reviewed
Shuji Iritani, Hirotaka Sekiguchi, Chikako Habuchi, Yohta Torii, Keisuke Kuroda, Kozo Kaibuchi, Norio Ozaki
NEUROSCIENCE RESEARCH Vol. 71 page： E392 - E392 2011
Exploring the molecules interacting with Neuregulin1 cytoplasmic domain Reviewed
Ryo Kuwata, Daisuke Mori, Daisuke Tsuboi, Tomoki Nishioka, Yukihiko Iizuka, Keisuke Kuroda, Hisashi Yano, Shinpei Kubota, Kozo Kaibuchi
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 115 page： 143P - 143P 2011
Migration defects by DISC1 knockdown in C57BL/6, 129X1/SvJ, and ICR strains via in utero gene transfer and virus-mediated RNAi Reviewed Open Access
Ken-ichiro Kubo, Kenji Tomita, Asuka Uto, Keisuke Kuroda, Saurav Seshadri, Jared Cohen, Kozo Kaibuchi, Atsushi Kamiya, Kazunori Nakajima
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS Vol. 400 ( 4 ) page： 631 - 637 2010.10
Analysis of DISC1 using knockout mice and new antibodies Reviewed
Kuroda, K., Mori, D., Namba, T., Tsuboi, D., Yano, H., Ibi, D., Tanaka, M., Isotani, M., Enomoto, A., Yamada, K., Sokabe, M., Takahashi, M., Kaibuchi, K.
Society for Neuroscience Abstract Viewer and Itinerary Planner Vol. 40 2010
Jaesuk Yun, Taku Nagai, Yoko Hibi, Hiroyuki Koike, Atumi Nitta, Keisuke Kuroda, Kozo Kaibuchi, Kiyofumi Yamada
NEUROSCIENCE RESEARCH Vol. 68 page： E251 - E251 2010
Phenotypic analysis of DISC1 knockout mice Reviewed
Keisuke Kuroda, Daisuke Mori, Shinichiro Taya, Daisuke Tsuboi, Takashi Namba, Ryo Kuwata, Hisashi Yano, Shinpei Kubota, Takafumi Kinoshita, Daisuke Ibi, Taku Nagai, Kiyofumi Yamada, Motoki Tanaka, Masahiro Sokabe, Mayu Isotani, Atsushi Enomoto, Masahide Takahashi, Hiroshi Kiyonari, Takaya Abe, Kozo Kaibuchi
NEUROSCIENCE RESEARCH Vol. 68 page： E200 - E200 2010
Npas4 regulates neuronal differentiation induced by lithium chloride in Neuro2a cells Reviewed
Jaesuk Yu, Taku Nagai, Yoko Hibi, Hiroyuki Koike, Atumi Nitta, Keisuke Kuroda, Kozo Kaibuchi, Kiyofumi Yamada
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 112 page： 71P - 71P 2010
Proteomic analysis reveals novel binding partners of dysbindin, a schizophrenia-related protein Reviewed Open Access
Takao Hikita, Shinichiro Taya, Yasutaka Fujino, Setsuko Taneichi-Kuroda, Kanae Ohta, Daisuke Tsuboi, Tomoyasu Shinoda, Keisuke Kuroda, Yusuke Funahashi, Junko Uraguchi-Asaki, Ryota Hashimoto, Kozo Kaibuchi
JOURNAL OF NEUROCHEMISTRY Vol. 110 ( 5 ) page： 1567 - 1574 2009.9
Roles of Disrupted-In-Schizophrenia 1-Interacting Protein Girdin in Postnatal Development of the Dentate Gyrus Reviewed Open Access
Atsushi Enomoto, Naoya Asai, Takashi Namba, Yun Wang, Takuya Kato, Motoki Tanaka, Hitoshi Tatsumi, Shinichiro Taya, Daisuke Tsuboi, Keisuke Kuroda, Naoko Kaneko, Kazunobu Sawamoto, Rieko Miyamoto, Mayumi Jijiwa, Yoshiki Murakumo, Masahiro Sokabe, Tatsunori Seki, Kozo Kaibuchi, Masahide Takahashi
NEURON Vol. 63 ( 6 ) page： 774 - 787 2009.9
Pathophysiological functions of DISC1 as a component of RNA granule Reviewed
Daisuke Tsuboi, Keisuke Kuroda, Yasutaka Fujino, Kozo Kaibuchi
NEUROSCIENCE RESEARCH Vol. 65 page： S63 - S63 2009
Regulation of DISC1 through phosphorylation by ERK Reviewed
Keisuke Kuroda, Shinichiro Taya, Junko Uraguchi, Yusuke Funahashi, Kozo Kaibuchi
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 106 page： 140P - 140P 2008
DISC1 regulates the transport of the NUDEL/LIS1/14-3-3 epsilon complex through Kinesin-1 Reviewed Open Access
Shinichiro Taya, Tomoyasu Shinoda, Daisuke Tsuboi, Junko Asaki, Kumiko Nagai, Takao Hikita, Setsuko Kuroda, Keisuke Kuroda, Mariko Shimizu, Shinji Hirotsune, Akihiro Iwamatsu, Kozo Kaibuchi
JOURNAL OF NEUROSCIENCE Vol. 27 ( 1 ) page： 15 - 26 2007.1
Regulation of DISC1 through phosphorylation by ERK Reviewed
Shinichiro Taya, Junko Uraguchi-Asaki, Keisuke Kuroda, Kozo Kaibuchi
NEUROSCIENCE RESEARCH Vol. 58 page： S65 - S65 2007
リン酸化プロテオミクスが紐解く情動学習の分子基盤と精神疾患病態
船橋靖広, 船橋靖広, UDDIN Ahammad Rijwan, 張心健, 河谷昌泰, 吉見陽, 坪井大輔, 坪井大輔, 西岡朋生, 西岡朋生, 黒田啓介, 野田幸裕, 山田清文, 崎村建司, 永井拓, 山下貴之, 内野茂夫, 貝淵弘三, 貝淵弘三
次世代薬理学セミナー要旨集(Web) Vol. 2025 2025
NMDA受容体依存性のリン酸化プロテオームが忌避学習のための後シナプス形成を制御する
船橋靖広, 船橋靖広, AHAMMAD Rijwan Uddin, AHAMMAD Rijwan Uddin, 張心健, HOSSEN Emran, HOSSEN Emran, 河谷昌泰, 吉見陽, 吉見陽, 呉敏華, 王緩緩, 王緩緩, 李旭, 李旭, 坪井大輔, 坪井大輔, 西岡朋生, 西岡朋生, 黒田啓介, 天野睦紀, 野田幸裕, 野田幸裕, 山田清文, 崎村建司, 永井拓, 山下貴之, 内野茂夫, 貝淵弘三, 貝淵弘三
日本生物学的精神医学会(Web) Vol. 46th 2024
Unraveling the Phosphoprotein Network: Roles and Functions of NMDAR Cascades in Synaptic Growth and Learning
船橋靖広, 船橋靖広, AHAMMAD Rijwan Uddin, AHAMMAD Rijwan Uddin, 張心健, EMRAN Hossen, EMRAN Hossen, 河谷昌泰, 吉見陽, 吉見陽, 呉敏華, 王緩緩, 王緩緩, 坪井大輔, 坪井大輔, 西岡朋生, 西岡朋生, 黒田啓介, 天野睦紀, 野田幸裕, 野田幸裕, 山田清文, 崎村建司, 永井拓, 山下貴之, 内野茂夫, 貝淵弘三, 貝淵弘三
日本薬理学雑誌 Vol. 158 ( Supplement ) 2023
Repetitive and compulsive-like behaviors lead to cognitive dysfunction in Disc1Δ2-3/Δ2-3 mice. Reviewed
Wulaer, B, Nagai T, Sobue, A, Itoh, N, Kuroda, K, Kaibuchi, K, Nabeshima, T, Yamada, K
Gene Brain Behav page： e12478 2018.4
黒田啓介
日本生物学的精神医学会誌 Vol. 28 ( 3 ) page： 127‐131 - 131 2017.9
Dopamine-induced phosphorylation of NPAS4 through MAPK regulates reward-related learning and memory
Y. Funahashi, A. Ariza, K. Suzuki, S. Wei, S. Kozawa, T. Takano, K. Kuroda, T. Nagai, K. Kaibuchi
JOURNAL OF NEUROCHEMISTRY Vol. 142 page： 129 - 129 2017.8
Phosphorylation signals in hippocampal and striatal synapses
K. Kaibuchi, K. Kuroda, M. Yoshikawa
JOURNAL OF NEUROCHEMISTRY Vol. 142 page： 22 - 22 2017.8
KANPHOS (Kinase-Associated Phospho-Signaling) Platform - A database for neural phosphoproteomics with quality control
Keisuke Kuroda, Taku Nagai, Mutsuki Amano, Junichiro Yoshimoto, Takayuki Kannon, Tomoki Nishioka, Shiro Usui, Kozo Kaibuchi
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 133 ( 3 ) page： S263 - S263 2017.3
Phosphorylation signals in striatal medium spiny neurons. Invited Reviewed
Nagai, T, Yoshimoto, J, Kannon, T, Kuroda, K, Kaibuchi, K
Trends Pharmacol. Sci. Vol. 37 page： 858–871 2016.10
Dopamine phosphorylates GEF-H1 through PKA to regulate GEF-H 1 activity in striatum
Xinjian Zhang, Keisuke Kuroda, Hiroyuki Takenaka, Kaishu Oda, Reon Kondo, Tomoki Nishioka, Shinichi Nakamuta, Taku Nagai, Kozo Kaibuchi
JOURNAL OF PHARMACOLOGICAL SCIENCES Vol. 130 ( 3 ) page： S194 - S194 2016.3
Phosphoproteomics of the Dopamine Pathway Enables Discovery of Rap1 Activation as a Reward Signal In Vivo Reviewed
Taku Nagai, Shinichi Nakamuta, Keisuke Kuroda, Sakura Nakauchi, Tomoki Nishioka, Tetsuya Takano, Xinjian Zhang, Daisuke Tsuboi, Yasuhiro Funahashi, Takashi Nakano, Junichiro Yoshimoto, Kenta Kobayashi, Motokazu Uchigashima, Masahiko Watanabe, Masami Miura, Akinori Nishi, Kazuto Kobayashi, Kiyofumi Yamada, Mutsuki Amano, Kozo Kaibuchi
Neuron Vol. 89 ( 3 ) page： 550 - 565 2016.2
注目の遺伝子(第31回)統合失調症発症脆弱性分子DISC1の研究から明らかになった課題と新機能
坪井 大輔, 森 大輔, 黒田 啓介
分子精神医学 Vol. 15 ( 4 ) page： 301 - 303 2015.10
精神神経疾患の原因・関連遺伝子に関する基盤的開発研究
星野幹雄, 貝淵弘三, 中島欽一, 岩本和也, 油谷浩幸, 内匠透, 松尾勲, 深田優子, 永井拓, 今吉格, 太田深秀, 井上高良, 須藤文和, 増山典久, 田谷真一郎, 山田真弓, 早瀬ヨネ子, 菅野幸江, 酒匂潤子, 井上由紀子, 一戸紀孝, 川口将史, 辻村啓太, 森大輔, 黒田啓介, 飯塚幸彦, JAESK Yun, 爪麻美
精神・神経疾患研究開発費による研究報告集(2年度班・初年度班) 平成24年度 page： 390-405,438-439 2013
疾患モデルと分子標的探索による治療薬開発 5.統合失調症の病因仮説とモデルマウス
黒田啓介, 飯塚幸彦, 貝淵弘三
実験医学 Vol. 30 ( 2 ) page： 377 - 381 2012.2
DISC1ノックアウトマウスにおけるGABAおよびドーパミン作動性神経系の解析
中井剛, 永井拓, 王蕊, 山田真之亮, 黒田啓介, 貝淵弘三, 山田清文
日本薬理学会近畿部会プログラム・要旨集 Vol. 122nd page： 35 2012
DISC1ノックアウトマウスの行動薬理学的解析
山田真之亮, 永井拓, 黒田啓介, 衣斐大祐, 王蕊, 貝淵弘三, 山田清文
日本アルコール・薬物医学会雑誌 Vol. 46 ( 4 ) page： 131 2011.8
新規蛍光Ca<sup>2+</sup>センサーG‐CaMP4を用いたレンチウイルスベクター遺伝子導入法による細胞内Ca<sup>2+</sup>動態の可視化
中井剛, 永井拓, 榎本篤, 中牟田信一, 黒田啓介, 難波隆志, 日置寛之, 貝淵弘三, 中井淳一, 山田清文
日本薬理学会近畿部会プログラム・要旨集 Vol. 120th page： 56 2011
高次脳機能におけるDISC1遺伝子の役割
山田真之亮, 永井拓, 黒田啓介, 衣斐大祐, 王蕊, 貝淵弘三, 山田清文
日本臨床精神神経薬理学会・日本神経精神薬理学会合同年会プログラム・抄録集 Vol. 21st-41st page： 179 2011
統合失調症発症脆弱因子DISC1の神経細胞における局在と機能解析
久保田晋平, 森大輔, 黒田啓介, 坪井大輔, 貝淵弘三
生化学 page： ROMBUNNO.2P-0958 2010
統合失調症の神経発達障害とDISC1
坪井大輔, 黒田啓介, 貝淵弘三
医学のあゆみ Vol. 231 ( 10 ) page： 1023 - 1027 2009.12
Pathophysiological functions of DISC1
Vol. 231 ( 10 ) page： 1023 - 1027 2009.12
統合失調症の分子精神医学 DISC1と統合失調症
田谷真一郎, 黒田啓介, 貝淵弘三
分子精神医学 Vol. 7 ( 4 ) page： 341 - 346 2007.10
DISC1と統合失調症 (特集 統合失調症の分子精神医学)
田谷 真一郎, 黒田 啓介, 貝淵 弘三
分子精神医学 Vol. 7 ( 4 ) page： 341 - 346 2007.10
NCAM結合タンパク質の同定
黒田啓介, 中川誠人, 則竹淳, 西村隆, 深田優子, 深田正紀, 貝淵弘三
日本細胞生物学会大会講演要旨集 Vol. 54th page： 93 2001
Cell-type-specific isolation of 14-3-3 associated phosphoprotein from complex brain tissues
Yoshikawa, M; Kuroda, K; Nagai, T; Kaibuchi, K
JOURNAL OF PHARMACOLOGICAL SCIENCES 2017.3
Cell-type-specific isolation of 14-3-3 associated phosphoprotein from complex brain tissues. International conference
Yoshikawa, M; Kuroda, K; Nagai, T; Kaibuchi, K
MOLECULAR BIOLOGY OF THE CELL 2017
Cell type specific analysis of intracellular signal in antipsychotics
Grant number：20K07965 2020.4 - 2023.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Authorship：Principal investigator
Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）
Analysis of RhoA/Rho-kinase signals in neuronal circuit
Grant number：18K14816 2018.4 - 2022.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Early-Career Scientists
KURODA Keisuke
Authorship：Principal investigator
Grant amount：\4160000 （ Direct Cost: \3200000 、 Indirect Cost：\960000 ）
We analyzed the intracellular signaling of RhoA, a Rho family low molecular weight G protein, and its downstream molecule, Rho-kinase, in neurons. We found that the activator (GEF) and inactivator (GAP) of RhoA were phosphorylated in a neural activity-dependent manner. We observed changes in synaptic morphology and behavior in mice injected with AAV, which regulates the activity of RhoA and Rho-kinase, and in double-conditional knockout mice of Rho-kinase (Rock1 and Rock2). These findings suggest that neurons regulate synaptic morphology and behavior via RhoA and Rho-kinase signaling.
Study on regulatory mechanism of neural activity and emotional behavior by intracellular signals
Grant number：17H01380 2017.4 - 2021.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
Kaibuchi Kozo
Authorship：Coinvestigator(s)
In this study, we comprehensively identified proteins that are phosphorylated downstream of neuromodulators (dopamine, adenosine, acetylcholine) and glutamate using our proprietary phospho-proteomics method. We have elucidated the intracellular signal transduction that regulates neuronal excitatory, synaptic plasticity, and gene expression at the molecular level, and also elucidated the regulatory mechanism of emotional behavior and its learning & memory.
Elucidation of regulation mechanism of gene expression and emotional memory by dopamine signal
Grant number：16K18393 2016.4 - 2019.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
Funahashi Yasuhiro, Kaibuchi Kozo, Nagai Taku, Amano Mutsuki, Nishioka Tomoki, Kuroda Keisuke
How dopamine regulates reward-related learning and memory through gene expression is poorly understood. Here, to identify the relevant transcriptional factors, we performed proteomic analysis using affinity beads coated with CREB-binding protein (CBP), a transcriptional coactivator involved in reward-related behavior. We identified more than 100 CBP-interacting proteins, including Neuronal Per-Arnt-Sim domain protein 4 (Npas4). We also found that MAPK phosphorylated Npas4 downstream of PKA, increasing Npas4-CBP interaction and the transcriptional activity of Npas4 at the brain-derived neurotrophic factor (BDNF) promoter. Deletion of Npas4 in the D1R-expressing MSNs impaired cocaine-induced place preference, which was rescued by Npas4-WT but not by a phospho-deficient Npas4 mutant. These observations suggest that MAPK phosphorylates Npas4 in D1R-MSNs and increases its transcriptional activity to enhance reward-related learning and memory.
Cell type specific analysis of intracellular signal in neural circuit
Grant number：15K06772 2015.4 - 2019.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Kuroda Keisuke
Authorship：Principal investigator
Grant amount：\5200000 （ Direct Cost: \4000000 、 Indirect Cost：\1200000 ）
Dopamine activates PKA via D1 receptor / Gαs pathway in D1R-MSN of the nucleus accumbens. On the other hand, the adenosine / A2A receptor / Gαs pathway and the dopamine / D2 receptor / Gαi pathway are antagonized in D2R-MSN. The D2 receptor is constantly activated by dopamine, and when dopamine secretion decreases, repression is released and PKA is activated in D2R-MSN. Activated PKA phosphorylates and activates RasGRP2, which is an activator of Rap1 (GEF), and phosphorylates and inactivates Rap1GAP, which is an inactivator of Rap1 (GAP). PKA activates Rap1 / MAPK signals and enhances neuronal excitability.
Elucidation of phosphorylation signal regulating the cell polarity
Grant number：25251021 2013.4 - 2016.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (A)
Kaibuchi Kozo
The establishment and maintenance of cell polarity is essential to a wide range of biological processes, including axon formation and cell migration. In elucidating of molecular basis of cell polarization, cell polarity proteins such as PAR complex, and Scrib complex have been studied. In these studies, various kinases have been shown to act as cell polarity regulators. However, how these kinases regulate cell polarization is poorly understood. In this study, we used our recently developed proteomic system and identified numerous candidate substrates downstream of polarity-related kinases. Based on the substrate screening results, we analyzed the spatiotemporal dynamics and function of the identified substrates during cell migration. Recently, we also revealed how neurons can grow only a single axon while preventing the formation of unnecessary axons during neuronal polarization.
Re-evaluation of DISC1 using knockout mice and new antibodies
Grant number：25830051 2013.4 - 2015.3
Japan Society for the Promotion of Science Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)
KURODA Keisuke
Disrupted-In-Schizophrenia 1 (DISC1) is a promising candidate gene for susceptibility to psychiatric disorders, including schizophrenia. We previously generated a DISC1 knockout (KO) mouse and developed DISC1 antibodies. We found that no available antibodies to DISC1 without us recognized the endogenous DISC1. The DISC1 KO mouse displays no gross abnormalities, but it does show altered synaptic plasticity and abnormal emotional behaviors. So that we re-evaluate DISC1 using KO mice and new antibodies and investigate true function of DISC1 in synapse.
We found that cell migration defect coused by DISC1 knockdown is an off-target effect of shRNA. This result suggests that DISC1 studies using DISC1 shRNAs should be interpreted with caution.
We used proteomic analysis to screen for DISC1 interactors and identified several RNA-binding proteins. We found that DISC1 binds ITPR1 mRNA with HZF, thereby regulating its dendritic transport for synaptic plasticity.
