Updated on 2022/05/12

写真a

 
KIRYU-SEO Sumiko
 
Organization
Graduate School of Medicine Program in Integrated Medicine Anatomy and Cell Biology Associate professor
Graduate School
Graduate School of Medicine
Undergraduate School
School of Medicine
Title
Associate professor
Contact information
メールアドレス

Degree 2

  1. 医学博士 ( 1998.3   大阪大学 ) 

  2. 医科学修士 ( 1994.3   大阪大学 ) 

Research Interests 10

  1. 神経変性

  2. 軸索輸送

  3. ミトコンドリア

  4. メタロプロテアーゼ

  5. 神経筋接合部

  6. グリア細胞

  7. 軸索

  8. 運動ニューロン

  9. 神経再生

  10. ストレス応答

Research Areas 1

  1. Life Science / Neuroscience-general

Current Research Project and SDGs 1

  1. 神経再生•変性に関わる分子メカニズム

Research History 6

  1. Nagoya University   Graduate School of Medicine Program in Integrated Medicine Anatomy and Cell Biology   Associate professor

    2011.8

  2. 大阪市立大学・大学院医学研究科・准教授

    2008.7 - 2011.7

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    Country:Japan

  3. 大阪市立大学・大学院医学研究科・講師

    2004.10 - 2008.6

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    Country:Japan

  4. 大阪市立大学・大学院医学研究科・助手

    2001.4 - 2004.9

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    Country:Japan

  5. 旭川医科大学・助手

    1998.10 - 2001.4

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    Country:Japan

  6. 日本学術振興会特別研究員(PD)

    1998.4 - 1998.9

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    Country:Japan

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Education 1

  1. Osaka University   Graduate School, Division of Medicine   neuroscience

    - 1998.3

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    Country: Japan

Professional Memberships 5

  1. 北米神経科学会

  2. 日本解剖学会

  3. 日本神経科学会

  4. 日本神経化学会

  5. 北米神経科学会

Committee Memberships 3

  1. 日本解剖学会   学術委員会  

    2020.4   

  2. 日本神経化学会   ダイバーシティー推進委員会委員  

    2017.4 - 2019.3   

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    Committee type:Academic society

  3. 日本解剖学会   情報技術委員会  

    2015.4 - 2019.3   

Awards 3

  1. 大阪市医学会市長賞

    2008.2   大阪市医学会  

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    Country:Japan

  2. 大阪市医学会市長賞

    2007.2   大阪市医学会  

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    Country:Japan

  3. 日本解剖学会奨励賞

    2004.8   日本解剖学会  

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    Country:Japan

 

Papers 70

  1. Mitochondrial behavior during axon regeneration/degeneration in vivo.

    Kiryu-Seo S, Kiyama H

    Neuroscience research     2018.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.neures.2018.08.014

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  2. Damage-induced neuronal endopeptidase (DINE) enhances axonal regeneration potential of retinal ganglion cells after optic nerve injury

    Kaneko Aoi, Kiryu-Seo Sumiko, Matsumoto Sakiko, Kiyama Hiroshi

    CELL DEATH & DISEASE   Vol. 8 ( 6 ) page: e2847   2017.6

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    DOI: 10.1038/cddis.2017.212

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  3. ECEL1 mutation implicates impaired axonal arborization of motor nerves in the pathogenesis of distal arthrogryposis

    Nagata Kenichi, Kiryu-Seo Sumiko, Tamada Hiromi, Okuyama-Uchimura Fumi, Kiyama Hiroshi, Saido Takaomi C.

    ACTA NEUROPATHOLOGICA   Vol. 132 ( 1 ) page: 111-126   2016.7

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    DOI: 10.1007/s00401-016-1554-0

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  4. Axonal injury alters the extracellular glial environment of the axon initial segment and allows substantial mitochondrial influx into axon initial segment Reviewed

    Hiromi Tamada, Sumiko Kiryu-Seo, Sohgo Sawada, Hiroshi Kiyama

    J Comp Neurol   Vol. 529 ( 16 ) page: 3621 - 3632   2021.11

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  5. Axonal injury alters the extracellular glial environment of the axon initial segment and allows substantial mitochondrial influx into axon initial segment

    Tamada Hiromi, Kiryu-Seo Sumiko, Sawada Sohgo, Kiyama Hiroshi

    JOURNAL OF COMPARATIVE NEUROLOGY   Vol. 529 ( 16 ) page: 3621 - 3632   2021.11

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/cne.25212

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  6. Zonisamide ameliorates neuropathic pain partly by suppressing microglial activation in the spinal cord in a mouse model

    Koshimizu Hiroyuki, Ohkawara Bisei, Nakashima Hiroaki, Ota Kyotaro, Kanbara Shunsuke, Inoue Taro, Tomita Hiroyuki, Sayo Akira, Kiryu-Seo Sumiko, Konishi Hiroyuki, Ito Mikako, Masuda Akio, Ishiguro Naoki, Imagama Shiro, Kiyama Hiroshi, Ohno Kinji

    LIFE SCIENCES   Vol. 263   page: 118577   2020.12

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    DOI: 10.1016/j.lfs.2020.118577

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  7. TC10, a Rho family GTPase, is required for efficient axon regeneration in a neuron-autonomous manner Reviewed

    Koinuma Shingo, Negishi Ryota, Nomura Riko, Sato Kazuki, Kojima Takuya, Segi-Nishida Eri, Goitsuka Ryo, Iwakura Yoichiro, Wada Naoyuki, Koriyama Yoshiki, Kiryu-Seo Sumiko, Kiyama Hiroshi, Nakamura Takeshi

    JOURNAL OF NEUROCHEMISTRY     2020.11

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    DOI: 10.1111/jnc.15235

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  8. New Insights of a Neuronal Peptidase DINE/ECEL1: Nerve Development, Nerve Regeneration and Neurogenic Pathogenesis.

    Kiryu-Seo S, Nagata K, Saido TC, Kiyama H

    Neurochemical research     2018.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s11064-018-2665-x

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  9. Distinct functional consequences of ECEL1/DINE missense mutations in the pathogenesis of congenital contracture disorders

    Nagata Kenichi, Takahashi Mika, Kiryu-Seo Sumiko, Kiyama Hiroshi, Saido Takaomi C.

    ACTA NEUROPATHOLOGICA COMMUNICATIONS   Vol. 5 ( 1 ) page: 83   2017.11

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    DOI: 10.1186/s40478-017-0486-9

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  10. Three-dimensional analysis of somatic mitochondrial dynamics in fission-deficient injured motor neurons using FIB/SEM

    Tamada Hiromi, Kiryu-Seo Sumiko, Hosokawa Hiroki, Ohta Keisuke, Ishihara Naotada, Nomura Masatoshi, Mihara Katsuyoshi, Nakamura Kei-ichiro, Kiyama Hiroshi

    JOURNAL OF COMPARATIVE NEUROLOGY   Vol. 525 ( 11 ) page: 2535-2548   2017.8

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    DOI: 10.1002/cne.24213

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  11. Mitochondrial fission is an acute and adaptive response in injured motor neurons

    Kiryu-Seo Sumiko, Tamada Hiromi, Kato Yukina, Yasuda Katsura, Ishihara Naotada, Nomura Masatoshi, Mihara Katsuyoshi, Kiyama Hiroshi

    SCIENTIFIC REPORTS   Vol. 6   page: 28331   2016.6

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    DOI: 10.1038/srep28331

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  12. Motor Nerve Arborization Requires Proteolytic Domain of Damage-Induced Neuronal Endopeptidase (DINE) during Development

    Matsumoto Sakiko, Kiryu-Seo Sumiko, Kiyama Hiroshi

    JOURNAL OF NEUROSCIENCE   Vol. 36 ( 17 ) page: 4744-4757   2016.4

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    DOI: 10.1523/JNEUROSCI.3811-15.2016

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  13. The mitochondrial dynamics after neuronal injury

    Kiryu-Seo S., Kiyama H.

    JOURNAL OF NEUROCHEMISTRY   Vol. 134   page: 92-92   2015.8

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  14. Proteolipid protein cannot replace P0 protein as the major structural protein of peripheral nervous system myelin.

    Yin X, Kiryu-Seo S, Kidd GJ, Feltri ML, Wrabetz L, Trapp BD

    Glia   Vol. 63 ( 1 ) page: 66-77   2015.1

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    DOI: 10.1002/glia.22733

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  15. MICRORNA-124 IS DOWN REGULATED IN NERVE-INJURED MOTOR NEURONS AND IT POTENTIALLY TARGETS MRNAS FOR KLF6 AND STAT3

    Nagata K., Hama I., Kiryu-Seo S., Kiyama H.

    NEUROSCIENCE   Vol. 256   page: 426-432   2014.1

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    DOI: 10.1016/j.neuroscience.2013.10.055

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  16. Expression analysis of the regenerating gene (Reg) family members Reg-III beta and Reg-III gamma in the mouse during development

    Matsumoto Sakiko, Konishi Hiroyuki, Maeda Rie, Kiryu-Seo Sumiko, Kiyama Hiroshi

    JOURNAL OF COMPARATIVE NEUROLOGY   Vol. 520 ( 3 ) page: 479-494   2012.2

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    DOI: 10.1002/cne.22705

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  17. Cleavage of Neuregulin-1 by BACE1 or ADAM10 Protein Produces Differential Effects on Myelination

    Luo Xiaoyang, Prior Marguerite, He Wanxia, Hu Xiangyou, Tang Xiaoying, Shen Weizhen, Yadav Satya, Kiryu-Seo Sumiko, Miller Robert, Trapp Bruce D., Yan Riqiang

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 286 ( 27 ) page: 23967-23974   2011.7

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    DOI: 10.1074/jbc.M111.251538

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  18. Myelination and axonal electrical activity modulate the distribution and motility of mitochondria at CNS nodes of Ranvier.

    Ohno N, Kidd GJ, Mahad D, Kiryu-Seo S, Avishai A, Komuro H, Trapp BD

    The Journal of neuroscience : the official journal of the Society for Neuroscience   Vol. 31 ( 20 ) page: 7249-58   2011.5

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    DOI: 10.1523/JNEUROSCI.0095-11.2011

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  19. The nuclear events guiding successful nerve regeneration

    Kiryu-Seo Sumiko, Kiyama Hiroshi

    FRONTIERS IN MOLECULAR NEUROSCIENCE   Vol. 4   page: 53   2011

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    DOI: 10.3389/fnmol.2011.00053

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  20. Altered expression of mRNA for the low-density lipoprotein receptor (LDLR) in injured motor neurons of rodent

    Hama Isuzu, Kiryu-Seo Sumiko, Konishi Hiroyuki, Kim Dong-Ho, Saeki Shigeru, Kiyama Hiroshi

    NEUROSCIENCE RESEARCH   Vol. 71   page: E339 - E340   2011

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    DOI: 10.1016/j.neures.2011.07.1489

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  21. Damage-Induced Neuronal Endopeptidase Is Critical for Presynaptic Formation of Neuromuscular Junctions

    Nagata Kenichi, Kiryu-Seo Sumiko, Maeda Mitsuyo, Yoshida Kayo, Morita Takashi, Kiyama Hiroshi

    JOURNAL OF NEUROSCIENCE   Vol. 30 ( 20 ) page: 6954-6962   2010.5

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    DOI: 10.1523/JNEUROSCI.4521-09.2010

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  22. Demyelination increases axonal stationary mitochondrial size and the speed of axonal mitochondrial transport.

    Kiryu-Seo S, Ohno N, Kidd GJ, Komuro H, Trapp BD

    The Journal of neuroscience : the official journal of the Society for Neuroscience   Vol. 30 ( 19 ) page: 6658-66   2010.5

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    DOI: 10.1523/JNEUROSCI.5265-09.2010

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  23. Molecular characterization and expression of the low-density lipoprotein receptor-related protein-10, a new member of the LDLR gene family

    Jeong Young-Hee, Ishikawa Kayoko, Someya Yoshimi, Hosoda Akemi, Yoshimi Tomohiko, Yokoyama Chikako, Kiryu-Seo Sumiko, Kang Man-Jong, Tchibana Taro, Kiyama Hiroshi, Fukumura Tomoe, Kim Dong-Ho, Saeki Shigeru

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 391 ( 1 ) page: 1110-1115   2010.1

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    DOI: 10.1016/j.bbrc.2009.12.033

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  24. PROLONGED STRESS AFFECTS THE HYPOTHALAMIC DOPAMINE SYSTEM AND CAUSES DEGENERATION OF THE PITUITARY CELLS

    Ogawa T., Shishioh-Ikejima N., Konishi H., Makino T., Kiryu-Seo S., Tanaka M., Watanabe Y., Kiyama H.

    JOURNAL OF NEUROCHEMISTRY   Vol. 110   page: 196-196   2009.9

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  25. PROLONGED STRESS AFFECTS THE HYPOTHALAMIC DOPAMINE SYSTEM AND CAUSES DEGENERATION OF THE PITUITARY CELLS

    Ogawa T, Shishioh-Ikejima N, Konishi H, Makino T, Kiryu-Seo S, Tanaka M, Watanabe Y, Kiyama H

    JOURNAL OF NEUROCHEMISTRY   Vol. 110   page: 196-196   2009.9

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  26. Chronic stress elicits prolonged activation of alpha-MSH secretion and subsequent degeneration of melanotroph

    Ogawa Tokiko, Shishioh-Ikejima Nobue, Konishi Hiroyuki, Makino Tetsuya, Sei Hiroyoshi, Kiryu-Seo Sumiko, Tanaka Masaaki, Watanabe Yasuyoshi, Kiyama Hiroshi

    JOURNAL OF NEUROCHEMISTRY   Vol. 109 ( 5 ) page: 1389-1399   2009.6

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    DOI: 10.1111/j.1471-4159.2009.06057.x

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  27. G-Protein-Coupled Receptor Screen Reveals a Role for Chemokine Receptor CCR5 in Suppressing Microglial Neurotoxicity

    Gamo Kazushige, Kiryu-Seo Sumiko, Konishi Hiroyuki, Aoki Shunsuke, Matsushima Kouji, Wada Keiji, Kiyama Hiroshi

    JOURNAL OF NEUROSCIENCE   Vol. 28 ( 46 ) page: 11980-11988   2008.11

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    DOI: 10.1523/JNEUROSCI.2920-08.2008

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  28. Neuronal injury-inducible gene is synergistically regulated by ATF3, c-Jun, and STAT3 through the interaction with Sp1 in damaged neurons

    Kiryu-Seo Sumiko, Kato Ryuichi, Ogawa Tokiko, Nakagomi Saya, Nagata Kenichi, Kiyama Hiroshi

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 283 ( 11 ) page: 6988-6996   2008.3

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    DOI: 10.1074/jbc.M707514200

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  29. Suture of transected nerve suppresses expression of BH3-only protein noxa in nerve-transected motor neurons of C57BL/6J mouse

    Gamo Kazushige, Kiryu-Seo Sumiko, Yoshikawa Hideki, Kiyama Hiroshi

    JOURNAL OF NEUROTRAUMA   Vol. 24 ( 5 ) page: 876-884   2007.5

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    DOI: 10.1089/neu.2006.0187

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  30. Altered expression of Smad family members in injured motor neurons of rat

    Okuyama Noriko, Kiryu-Seo Sumiko, Kiyama Hiroshi

    BRAIN RESEARCH   Vol. 1132 ( 1 ) page: 36-41   2007.2

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    DOI: 10.1016/j.brainres.2006.11.019

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  31. Unique anti-apoptotic activity of EAAC1 in injured motor neurons

    Kiryu-Seo Sumiko, Gamo Kazushige, Tachibana Taro, Tanaka Kohichi, Kiyama Hiroshi

    EMBO JOURNAL   Vol. 25 ( 14 ) page: 3411-3421   2006.7

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    DOI: 10.1038/sj.emboj.7601225

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  32. Identification and functional analysis of damage-induced neuronal endopeptidase (DINE), a nerve injury associated molecule

    Kiryu-Seo Sumiko

    ANATOMICAL SCIENCE INTERNATIONAL   Vol. 81 ( 1 ) page: 1-6   2006.3

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  33. Unique anti-apoptotic activity of EAAC1 in injured motor neurons. Reviewed

    Kiryu-Seo S, Gamo K, Tachibana T, Tanaka, K and Kiyama H.

    EMBO J   Vol. 25   page: 3411-3421   2006

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  34. Localization and ontogeny of damage-induced neuronal endopeptidase mRNA-expressing neurons in the rat nervous system

    Nagata K., Kiryu-Seo S., Kiyama H.

    NEUROSCIENCE   Vol. 141 ( 1 ) page: 299-310   2006

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    DOI: 10.1016/j.neuroscience.2006.03.032

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  35. Transcriptional regulation of DINE in response to nerve injury

    Kiryu-Seo Sumiko, Kato Ryuichi, Kiyama Hiroshi

    NEUROSCIENCE RESEARCH   Vol. 55   page: S218 - S218   2006

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  36. Induced expressions of Rab24 GTPase and LC3 in nerve-injured motor neurons

    Egami Y, Kiryu-Seo S, Yoshimori T, Kiyama H

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 337 ( 4 ) page: 1206-1213   2005.12

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    DOI: 10.1016/j.bbrc.2005.09.171

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  37. Altered expression of neprilysin family members in the pituitary gland of sleep-disturbed rats, an animal model of severe fatigue

    Ogawa T, Kiryu-Seo S, Tanaka M, Konishi H, Iwata N, Saido T, Watanabe Y, Kiyama H

    JOURNAL OF NEUROCHEMISTRY   Vol. 95 ( 4 ) page: 1156-1166   2005.11

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    DOI: 10.1111/j.1471-4159.2005.03436.x

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  38. Altered expression of Neprilysin family members in pituitary gland of chronically sleep disturbed rat

    Ogawa T, Kiryu-Seo S, Tanaka M, Iwata N, Saido TC, Watanabe Y, Kiyama H

    JOURNAL OF NEUROCHEMISTRY   Vol. 94   page: 242-242   2005.8

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  39. The p53-independent nuclear translocation of cyclin G1 in degenerating neurons by ischemic and traumatic insults

    Maeda M, Ampo KI, Kiryu-Seo S, Konishi H, Ohba N, Kadono C, Kiyama H

    EXPERIMENTAL NEUROLOGY   Vol. 193 ( 2 ) page: 350-360   2005.6

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    DOI: 10.1016/j.expneurol.2005.01.018

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  40. Noxa is a critical mediator of p53-dependent motor neuron death after nerve injury in adult mouse

    Kiryu-Seo S, Hirayama T, Kato R, Kiyama H

    JOURNAL OF NEUROSCIENCE   Vol. 25 ( 6 ) page: 1442-1447   2005.2

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    DOI: 10.1523/JNEUROSCI.4041-04.2005

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  41. Expression of damage-induced neuronal endopeptidase (DINE) mRNA in peri-infarct cortical and thalamic neurons following middle cerebral artery occlusion

    Ohba N, Kiryu-Seo S, Maeda M, Muraoka M, Ishii M, Kiyama H

    JOURNAL OF NEUROCHEMISTRY   Vol. 91 ( 4 ) page: 956-964   2004.11

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    DOI: 10.1111/j.1471-4159.2004.02784.x

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  42. Dine (damage induced neuronal endopeptidase)

    Kiryu-Seo S, Kiyama H

    PROTEIN AND PEPTIDE LETTERS   Vol. 11 ( 5 ) page: 451-460   2004.10

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  43. Transgenic mouse overexpressing the Akt reduced the volume of infarct area after middle cerebral artery occlusion

    Ohba N, Kiryu-Seo S, Maeda M, Muraoka M, Ishii M, Kiyama H

    NEUROSCIENCE LETTERS   Vol. 359 ( 3 ) page: 159-162   2004.4

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    DOI: 10.1016/j.neulet.2004.02.029

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  44. Critical role for DP5/Harakiri, a Bcl-2 homology domain 3-only Bcl-2 family member, in axotomy-induced neuronal cell death

    Imaizumi K, Benito A, Kiryu-Seo S, Gonzalez V, Inohara N, Leiberman AP, Kiyama H, Nunez G

    JOURNAL OF NEUROSCIENCE   Vol. 24 ( 15 ) page: 3721-3725   2004.4

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    DOI: 10.1523/JNEUROSCI.5101-03.2004

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  45. Vesicular acetylcholine transporter can be a morphological marker for the reinnervation to muscle of regenerating motor axons

    Maeda M, Ohba N, Nakagomi S, Suzuki Y, Kiryu-Seo S, Namikawa K, Kondoh W, Tanaka A, Kiyama H

    NEUROSCIENCE RESEARCH   Vol. 48 ( 3 ) page: 305-314   2004.3

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    DOI: 10.1016/j.neures.2003.11.008

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  46. Dine (damage induced neuronal endopeptidase) Invited Reviewed

    Kiryu-Seo S,. Kiyama H.

    Protein Pept. Lett.   Vol. 11   page: 451-460   2004

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  47. Cavernous nerve injury elicits GAP-43 mRNA expression but not regeneration of injured pelvic ganglion neurons

    Kato R, Kiryu-Seo S, Sato Y, Hisasue S, Tsukamoto T, Kiyama H

    BRAIN RESEARCH   Vol. 986 ( 1-2 ) page: 166-173   2003.10

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    DOI: 10.1016/S0006-8993(03)03249-9

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  48. Collapsin response mediator protein-2 accelerates axon regeneration of nerve-injured motor neurons of rat

    Suzuki Y, Nakagomi S, Namikawa K, Kiryu-Seo S, Inagaki N, Kaibuchi K, Aizawa H, Kikuchi K, Kiyama H

    JOURNAL OF NEUROCHEMISTRY   Vol. 86 ( 4 ) page: 1042-1050   2003.8

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    DOI: 10.1046/j.1471-4159.2003.01920.x

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  49. Expression of the activating transcription factor 3 prevents c-Jun N-terminal kinase-induced neuronal death by promoting heat shock protein 27 expression and Akt activation.

    Nakagomi S, Suzuki Y, Namikawa K, Kiryu-Seo S, Kiyama H

    The Journal of neuroscience : the official journal of the Society for Neuroscience   Vol. 23 ( 12 ) page: 5187-96   2003.6

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  50. Expression of the activating transcription factor 3 prevents c-Jun N-terminal kinase-induced neuronal death by promoting heat shock protein 27 expression and Akt activation

    Nakagomi S, Suzuki Y, Namikawa K, Kiryu-Seo S, Kiyama H

    JOURNAL OF NEUROSCIENCE   Vol. 23 ( 12 ) page: 5187-5196   2003.6

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  51. Identification of an axotomy-induced glycosylated protein, AIGP1, possibly involved in cell death triggered by endoplasmic reticulum-Golgi stress.

    Aoki S, Su Q, Li H, Nishikawa K, Ayukawa K, Hara Y, Namikawa K, Kiryu-Seo S, Kiyama H, Wada K

    The Journal of neuroscience : the official journal of the Society for Neuroscience   Vol. 22 ( 24 ) page: 10751-60   2002.12

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    PubMed

  52. Damage-induced neuronal endopeptidase (DINE/ECEL) expression is regulated by leukemia inhibitory factor and deprivation of nerve growth factor in rat sensory ganglia after nerve injury

    Kato R, Kiryu-Seo S, Kiyama H

    JOURNAL OF NEUROSCIENCE   Vol. 22 ( 21 ) page: 9410-9418   2002.11

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    Web of Science

  53. Neprilysin degrades both amyloid beta peptides 1-40 and 1-42 most rapidly and efficiently among thiorphan- and phosphoramidon-sensitive endopeptidases

    Shirotani K, Tsubuki S, Iwata N, Takaki Y, Harigaya W, Maruyama K, Kiryu-Seo S, Kiyama H, Iwata H, Tomita T, Iwatsubo T, Saido TC

    JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 276 ( 24 ) page: 21895-21901   2001.6

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    Web of Science

  54. A disintegrin and metalloprotease with thrombospondin type1 motifs (ADAMTS-1) and IL-1 receptor type 1 mRNAs are simultaneously induced in nerve injured motor neurons

    Sasaki M, Seo-Kiryu S, Kato R, Kita S, Kiyama H

    MOLECULAR BRAIN RESEARCH   Vol. 89 ( 1-2 ) page: 158-163   2001.4

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    Web of Science

  55. Damage-induced neuronal endopeptidase (DINE) is a unique metallopeptidase expressed in response to neuronal damage and activates superoxide scavengers

    Kiryu-Seo S, Sasaki M, Yokohama H, Nakagomi S, Hirayama T, Aoki S, Wada K, Kiyama H

    PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA   Vol. 97 ( 8 ) page: 4345-4350   2000.4

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    Web of Science

  56. Endothelin-converting enzymes and endothelin receptor B messenger RNAs are expressed in different neural cell species and these messenger rnas are coordinately induced in neurons and astrocytes respectively following nerve injury

    Nakagomi S, Kiryu-Seo S, Kiyama H

    NEUROSCIENCE   Vol. 101 ( 2 ) page: 441-449   2000

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    Web of Science

  57. Discordant expression of c-Ret and glial cell line-derived neurotrophic factor receptor alpha-1 mRNAs in response to motor nerve injury in neonate rats

    Tsujino H, Mansur K, Kiryu-Seo S, Namikawa K, Kitahara T, Tanabe K, Ochi T, Kiyama H

    MOLECULAR BRAIN RESEARCH   Vol. 70 ( 2 ) page: 298-303   1999.7

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  58. Dimethylarginine dimethylaminohydrolase (DDAH) as a nerve-injury-associated molecule: mRNA localization in the rat brain and its coincident up-regulation with neuronal NO synthase (nNOS) in axotomized motoneurons

    Nakagomi S, Kiryu-Seo S, Kimoto M, Emson PC, Kiyama H

    EUROPEAN JOURNAL OF NEUROSCIENCE   Vol. 11 ( 6 ) page: 2160-2166   1999.6

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  59. Expressed-sequence-tag approach to identify differentially expressed genes following peripheral nerve axotomy

    Tanabe K, Nakagomi S, Kiryu-Seo S, Namikawa K, Imai Y, Ochi T, Tohyama M, Kiyama H

    MOLECULAR BRAIN RESEARCH   Vol. 64 ( 1 ) page: 34-40   1999.1

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  60. A sequence-specific splicing activator, Tra2 beta, is up-regulated in response to nerve injury

    Kiryu-Seo S, Matsuo N, Wanaka A, Ogawa S, Tohyama M, Kiyama H

    MOLECULAR BRAIN RESEARCH   Vol. 62 ( 2 ) page: 220-223   1998.11

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  61. Up-regulation of thioredoxin expression in motor neurons after nerve injury

    Mansur K, Iwahashi Y, Kiryu-Seo S, Su Q, Namikawa K, Yodoi J, Kiyama H

    MOLECULAR BRAIN RESEARCH   Vol. 62 ( 1 ) page: 86-91   1998.11

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    Web of Science

  62. Enhancement of extracellular glutamate scavenge system in injured motoneurons

    Toki H, Namikawa K, Su QN, Kiryu-Seo S, Sato K, Kiyama H

    JOURNAL OF NEUROCHEMISTRY   Vol. 71 ( 3 ) page: 913-919   1998.9

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  63. Enhanced expression of 14-3-3 family members in injured motoneurons

    Namikawa K, Su QN, Kiryu-Seo S, Kiyama H

    MOLECULAR BRAIN RESEARCH   Vol. 55 ( 2 ) page: 315-320   1998.4

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  64. Alternative expression of Shc family members in nerve-injured motoneurons

    Tanabe K, Kiryu-Seo S, Nakamura T, Mori N, Tsujino H, Ochi T, Kiyama H

    MOLECULAR BRAIN RESEARCH   Vol. 53 ( 1-2 ) page: 291-296   1998.1

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  65. Selective upregulation of cytokine receptor subchain and their intracellular signalling molecules after peripheral nerve injury

    Yao GL, Kato H, Khalil M, Kiryu S, Kiyama H

    EUROPEAN JOURNAL OF NEUROSCIENCE   Vol. 9 ( 5 ) page: 1047-1054   1997.5

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  66. p53-independent cyclin G expression in a group of mature neurons and its enhanced expression during nerve regeneration

    Morita N, Kiryu S, Kiyama H

    JOURNAL OF NEUROSCIENCE   Vol. 16 ( 19 ) page: 5961-5966   1996.10

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  67. Nerve injury enhances rat neuronal glutamate transporter expression: Identification by differential display PCR

    Kiryu S, Yao GL, Morita N, Kato H, Kiyama H

    JOURNAL OF NEUROSCIENCE   Vol. 15 ( 12 ) page: 7872-7878   1995.12

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  68. UP-REGULATION OF FERRITIN HEAVY-CHAIN MESSENGER-RNA EXPRESSION IN THE RAT SKELETAL-MUSCLE AFTER DENERVATION - DETECTED BY MEANS OF DIFFERENTIAL DISPLAY

    KITAHARA T, KIRYU S, TAKEDA N, KUBO T, KIYAMA H

    NEUROSCIENCE RESEARCH   Vol. 23 ( 4 ) page: 353-360   1995.11

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  69. REGULATION OF MESSENGER-RNA EXPRESSION INVOLVED IN RAS AND PKA SIGNAL PATHWAYS DURING RAT HYPOGLOSSAL NERVE REGENERATION

    KIRYU S, MORITA N, OHNO K, MAENO H, KIYAMA H

    MOLECULAR BRAIN RESEARCH   Vol. 29 ( 1 ) page: 147-156   1995.3

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  70. UP-REGULATION OF ERK (MAP KINASE) AND MEM (MAP KINASE KINASE) TRANSCRIPTION AFTER RAT FACIAL-NERVE TRANSECTION

    KITAHARA T, KIRYU S, OHNO K, MORITA N, KUBO T, KIYAMA H

    NEUROSCIENCE RESEARCH   Vol. 20 ( 3 ) page: 275-280   1994.9

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    Web of Science

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MISC 3

  1. Noxa is a critical mediator of p53-dependent motor neuron death after nerve injury in adult mouse. Reviewed

    Kiryu-Seo S, Hirayama T, Kato R, Kiyama H

    J Neurosci   Vol. 25   page: 1442-1447   2005

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    Language:English   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)  

  2. Damage Induced Neuronal Endopeptidase (DINE/ECEL1) expression is regulated by LIF and deprivation of NGF in rat sensory ganglia after nerve injury. J. Reviewed

    Kato R, Kiryu-Seo S, Kiyama H

    J Neurosci   Vol. 22   page: 9410-9418   2002

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  3. p53 independent cyclin G expression in a group of mature neurons and its implication for nerve regeneration. Reviewed

    Morita N, Kiryu S, Kiyama H

    J Neurosci   Vol. 16   page: 5961-5966   1996

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Presentations 18

  1. 損傷運動ニューロンの軸索再生・変性に関わるプロテアソーム依存性ストレス応答

    桐生寿美子、松下鈴佳、田代善崇、高橋良輔、吉村武、井口洋平、勝野雅央、木山博資

    第10回名古屋大学医学系研究科・ 生理学研究所合同シンポジウム  2020.9.19 

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    Event date: 2020.9

    Presentation type:Oral presentation (general)  

  2. プロテアソームによるAISの可塑性制御は損傷運動ニューロン軸索のミトコンドリア輸送に関与する

    桐生寿美子、松下鈴佳、田代善崇、高橋良輔、吉村武、井口洋平、勝野雅央、木山博資

    第63回日本神経化学会大会  2020.9 

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    Event date: 2020.9

    Presentation type:Oral presentation (general)  

  3. 損傷運動ニューロンにおいてプロテアソームが関わる新たな神経再生メカニズム

    桐生寿美子、松下鈴佳、田代善崇、高橋良輔、吉村武、木山博資

    第125回日本解剖学会総会・全国学術集会  2020.3 

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    Event date: 2020.3

    Presentation type:Oral presentation (general)  

  4. The mitochondrial dynamics after motor neuronal injury International conference

    Sumiko Kiryu-Seo

    International Society for Neurochemistry 2015 

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    Event date: 2015.8

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:Australia  

  5. DINE functions as a protease required for the motor nerve terminal arborization and neuromuscular junction formation

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    Event date: 2015.3

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  6. Nerve injury-induced mitochondrial fission is an essential adaptive response to maintain neuronal survival and promote axonal regeneration International conference

    Sumiko Kiryu-Seo, Hiromi Tamada, Yukina Kato, Naotada Ishihara, Masatoshi Nomura, Katsuyoshi Mihara, Hiroshi Kiyama

    Neuroscience2014 

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    Event date: 2014.11

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  7. Enzymatic activity of Damage-induced neuronal endopeptidase/Endothelin-converting enzyme-like 1 (DINE/ECEL1) is crucial for neuromuscular junction formation during development. International conference

    Sakiko Matsumoto, Sumiko Kiryu-Seo, Hiroshi Kiyama

    Neuroscience2014 

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    Event date: 2014.11

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  8. DINEのプロテアーゼ活性は脊髄運動ニューロン軸索終末分枝及び神経筋接合部形成に必須である

    松本早紀子、桐生寿美子、木山博資

    第57回日本神経化学会大会 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:奈良   Country:Japan  

  9. Aberrant axonal arborization of motor nerves in Damage-Induced Neuronal Endopeptidase deficient limb International conference

    Nagata K, Kiryu-Seo S, Kiyama H, Saido TC.

    Cold spring harbor meeting 

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    Event date: 2014.9

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  10. The protease activity of DINE/ECEL1 is required for the motor nerve terminal arborization and the neuromuscular junction formation International conference

    Matsumoto S, Kiryu-Seo S, Kiyama H.

    6th special conference of the international society for neurochemistry  

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    Event date: 2014.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  11. 神経損傷に応答するミトコンドリア

    桐生寿美子, 木山博資

    第57回日本神経化学会 

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    Event date: 2014.9

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:奈良   Country:Japan  

  12. 軸索に存在するDINEはシュワン細胞と相互作用することにより適切な軸索ブランチングおよび神経筋接合部形成を促す。

    桐生寿美子, 松本早紀子, 木山博資

    日本神経科学会 

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    Event date: 2013.6

    Language:English   Presentation type:Poster presentation  

    Venue:京都   Country:Japan  

  13. DINE欠損運動神経の骨格筋支配の発生学的異常

    永田健一, 桐生寿美子, 斉藤貴志, 木山博資

    日本神経科学会 

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    Event date: 2013.6

    Language:English   Presentation type:Poster presentation  

    Venue:京都   Country:Japan  

  14. 損傷神経細胞の再生能力に関わる分子メカニズム

    桐生寿美子, 木山博資

    日本解剖学会 

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    Event date: 2013.3

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

    Venue:香川県   Country:Japan  

  15. 脱髄による軸索内ミトコンドリアのサイズと輸送速度の変化

    桐生寿美子, 大野伸彦, Grahame J. Kidd, 小室仁, Bruce D. Trapp

    Neuro2010 

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    Event date: 2010.9

    Language:English   Presentation type:Poster presentation  

    Venue:神戸   Country:Japan  

  16. The protease activity of DINE/ECEL1 is required for the motor nerve terminal arborization and the neuromuscular junction formation

    Matsumoto S, Kiryu-Seo S, Kiyama H

    6th special conference of the international society for neurochemistry  2014.9 

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  17. Injured motor neurons lose polarity in a proteasome–dependent manner to increase the mitochondrial transport into regenerative axons

    Sumiko Kiryu-Seo, Reika Matsushita, Yoshitaka Tashiro, Ryosuke Takahashi, Takeshi Yoshimura, Yohei Iguchi, Masahisa Katsuno, Hiroshi Kiyama

    2021.3.21 

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  18. The dynamics of the AIS during neuronal injury

    2021.10.1 

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Research Project for Joint Research, Competitive Funding, etc. 11

  1. 組織修復を神経因性に制御する新たなメカニズム

    2020.1

    三菱財団自然科学研究助成 

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    Grant type:Competitive

  2. 上皮組織損傷治癒を神経依存性に調節する新たなメカニズムの解明

    2018.3

    武田科学振興財団生命科学研究助成 

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    Grant type:Competitive

  3. 角膜創傷治癒を促す新たな分子メカニズム

    2017.11 - 2018.10

    日本医学会総会記念医学振興基金 

    桐生寿美子

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    Grant type:Competitive

  4. ダメージを受けた運動ニューロンの樹状突起ダイナミクスの解明

    2017.11 - 2018.10

    豊秋奨学会 

    桐生寿美子

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    Grant type:Competitive

  5. プロテアーゼDINEが視神経再生を促進するメカニズム

    2017.3 - 2018.4

    堀科学芸術振興財団研究助成 

    桐生寿美子

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    Grant type:Competitive

  6. 軸索-シュワン細胞間の相互作用を制御するDINE複合体蛋白質の同定

    2013.4 - 2016.3

    武田科学振興財団医学系研究助成 

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    Grant type:Competitive

  7. 神経損傷•修復過程におけるDINEの機能

    2007.4 - 2009.3

    奨励研究助成 

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    Grant type:Competitive

  8. DINEによる損傷神経細胞修復の分子メカニズム

    2007.4 - 2008.3

    研究助成 

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    Grant type:Competitive

  9. 神経損傷応答分子DINEのコンディショナルノックアウトマウス作製

    2006.4 - 2007.3

    基礎医学医療研究助成 

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    Grant type:Competitive

  10. 損傷運動ニューロンの生と死

    2005.4 - 2006.4

    学内共同研究 

  11. 疲労時下垂体に発現するG蛋白結合型受容体(GPCR)の機能的意義の解明へ向けて

    2005.4 - 2006.3

    学内共同研究 

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KAKENHI (Grants-in-Aid for Scientific Research) 12

  1. 損傷運動ニューロンのリプログラミングに関わる遺伝子発現制御機構の網羅的解析

    Grant number:21K19310  2107.9

    日本学術振興会  科研費  挑戦的研究(萌芽)

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    Authorship:Principal investigator 

  2. 上皮組織損傷をスイッチとして稼働する神経依存性の新たな創傷治癒メカニズム

    2018.4

    科学研究費補助金  基盤研究(B) 

    桐生 寿美子

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    Authorship:Principal investigator  Grant type:Competitive

  3. 損傷神経軸索内ミトコンドリアの品質管理のメカニズム

    2013.4 - 2016.3

    科学研究費補助金  基盤研究(C)

    桐生寿美子

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    Authorship:Principal investigator 

  4. 損傷神経軸索におけるミトコンドリアのダイナミクス

    2010.4 - 2012.3

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

  5. 神経再生現象を融合する分子発現制御のメカニズムの解析

    2005.4 - 2007.3

    科学研究費補助金  基盤研究(B)

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    Authorship:Coinvestigator(s) 

  6. 損傷運動ニューロンにおけるDNAメチル化と遺伝子発現制御

    2004.4 - 2006.3

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

  7. グリア・ニュ ロン相互作用による神経機能維持メカニズムとその加齢変化の解析

    Grant number:15082205  2003 - 2007

    科学研究費助成事業  特定領域研究

    木山 博資, 中込 咲綾, 小西 博之, 前田 光代, 桐生 寿美子, 濤川 一彦

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    Authorship:Coinvestigator(s) 

    グリア・ニューロン相互作用の変化を解析するために,各種神経損傷モデルを用いて,神経・グリア相互作用に関係するミクログリアの作用と各種分子の同定を行った。本年度は(1)ミクログリアの応答と神経細胞死の関連,(2)ミクログリアに発現するGPCRの神経機能維持に関する解析,を中心に行った。(1)マウス運動神経は軸索損傷により半数以上が緩やかな細胞死をする。この時のミクログリアの動態を電子顕微鏡で観察した所,ミクログリアの接着のない運動ニューロンが存在し,それらは細胞死に至ることが新たに明らかになった。このことは,軸索損傷後ミクログリアは運動ニューロンを取り囲み生存のための環境を作りだしている可能性を示唆していると考えられた。(2)約300の蛋白共役型受容体のプライマーと,神経損傷側と健常側の神経核から抽出したRNAを用い,神経再生過程で発現が上昇するGPCRのスクリーニングを行った。この結果得られた遺伝子のうち,CCR5に注目してCCR5のリガンドであるRantesの発現動態を解析した。この結果受容体はミクログリアにリガンドは損傷運動ニューロンで発現していることが明らかになった。また,CCR5ノックアウトマウスでは運動ニューロンの細胞死が加速した。このことから,軸索損傷後にミクログリアで発現するCCR5は損傷運動ニューロンとリガンドを介して運動ニューロンの生存にポジティブに作用していることが推測された。10年ほど前から使用していたPCR用のPCR Thermal Cyclerとデータ取り込みのための顕微鏡デジタルカメラがいずれも老朽化のため何れも本研究中に修理不能となり,研究の取りまとめに多大な影響をきたしたので,当初予定していなかったが,主な物品として急遽購入した。

  8. 損傷神経細胞におけるDINEのストレス応答機構の解析

    2002.2 - 2004.3

    科学研究費補助金  若手研究(B)

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    Authorship:Principal investigator 

  9. Neural species specific gene transfer by adenovirus vector.

    Grant number:12558087  2000 - 2001

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    KIYAMA Hiroshi, NAMIKAWA Kazuhiko, SEO Sumiko

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    We have attempted to establish the adenovirus-mediated gene-transfer system, which enables neural species-specific expression. For this aim, Cre-loxP system was employed. Neuron, astrocyte, or Schwann cell specific promoters were examined in vitro. For the neuron specific expression, the basic promoter for SCG10 was used, and additional neuron specific silencer elements (NRSE) were attached. This modification increased the neuron specificity significantly. For the astrocyte expression, GFAP promoter was selected. Those promoters were used for the expression of Cre, and additional adenovirus vector, which encodes loxP-Stuffer-polyA-loxP-EGFP sequence downstream of a strong general promoter, was constructed. By the co-infection of these two adenoviruses, neural specie specific expression was attempted. The promoters we constructed for neurons and astrocytes worked well, and showed very high selectivity in culture such as the brain derived primary culture. Next we examined the specificity of the expression in rat brain. The combination of the adenovirus vectors was injected into various brain regions. The selectivity and expression level of the neuron specific promoter was nice, however the minor differences in the expression level among the injected regions were observed. This difference may be due to the core promoter of SCG10 ; nevertheless the selectivity in various regions was good. For the astrocyte specific expression, both selectivity and expression level was relatively good, but a few neuronal cells expressing EGFP was also found. However the expression level in neuronal cells were low. We have not obtained the Schwann cell specific promoter yet, and the study is on going.

  10. 新規損傷神経関連遺伝子による生存・再生のメカニズム

    Grant number:12031201  2000

    特定領域研究(A)

    瀬尾 寿美子

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\1300000 ( Direct Cost: \1300000 )

    神経損傷応答遺伝子として得られたメタロプロテアーゼDINE(Damage induced neuronal endopeptidase)の解析を行った。昨年度までに我々はDINEが中枢・末梢神経系の軸索障害に対して鋭敏に発現応答すること、細胞死防御活性を持つことを明らかにしている。今年度はこの点に着目し、(1)神経細胞死防御のための細胞内シグナリング、(2)DINE遺伝子の5'上流領域の解析を行った。
    (1)DINEを介した細胞内シグナリング
    神経細胞でのDINE過剰発現を目的として、DINEを組み込んだアデノウイルスを作製した。神経細胞により近い分化PC12細胞にこれを過剰発現させたところ、NGF除去後のアポトーシスが有意に抑制された。これはDINEによりプロセッシングを受けた未同定の基質が、G蛋白共役型受容体を介して生存シグナルを活性化させるためであることが明らかになった。しかし、DINEの基質は未だ不明であること、DINE以外の生存シグナル活性化分子は極めて多く報告されていることから、DINE特有の機能を明らかにすることが今後の課題として残された。
    (2)DINE遺伝子5'上流領域の解析
    様々な長さのDINE遺伝子5'上流領域をレポーター遺伝子に結合させたコンストラクトを作製し、神経細胞、非神経細胞におけるプロモーター解析を行った。この結果、極めて特異的に神経細胞にプロモーター活性を示すことが明らかになった。同様のアデノウイルスを作製し、初代培養系やラット大脳皮質への遺伝子導入実験を行った結果、神経細胞特異的発現が証明された。さらに、サイトカイン刺激によりDINEプロモーター活性が上昇することから、これが神経障害後の鋭敏な発現応答の一因と考えられた。以上のことは、グリア細胞などが混在する神経系において、損傷を受けた神経細胞に標的を絞った遺伝子導入が可能となることを示唆するものである。

  11. 軸索損傷神経細胞の生存・再生の分子メカニズムの解析

    Grant number:11157202  1999

    特定領域研究(A)

    瀬尾 寿美子

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\1200000 ( Direct Cost: \1200000 )

    当研究室で既に確立しているディファレンシャル・ディスプレイにより新たな神経再生関連遺伝子探索を行ったところ、いくつもの候補クローンが得られた。昨年度のcDNA全長クローニングにより、得られた候補遺伝子のうちのひとつは新規膜一回貫通型メタロプロテアーゼであることが明らかとなっていたため今年度はその機能解析を中心に進めた。本分子の特徴は神経系特異的に発現していること、末梢・中枢神経軸索損傷に対して極めて効率良く発現応答する点であることから、我々はDINE(Damage Induced Neuronal Endopeptidase)と名付けた。DINEはECE(Endothelin Converting Enzyme)とファミリーを形成しており、ニューロペプチドやサイトカインなどの前駆体プロセッシング酵素である可能性が高い。これらの分子群の発現は障害後の神経細胞において顕著に上昇することが従来より知られており、障害神経細胞に対し何らかの保護作用を持つものと考えられている。そこで、培養細胞を用いてDINEの生存維持活性性を検討した。その結果DINE過剰発現細胞ではセラミドによる細胞死が軽減されることが明らかとなった。このような生命維持活性を示す理由の一つとして、フリーラジカルスカベンジャーであるSOD(superoxide dismutase)が活性化することを我々は見いだした。SODへ至るシグナリング経路は不明でありこの点が今後の課題とされる。またin vivoでのDINEの機能評価を行うため、現在作製したアデノウイルスを用いて神経障害モデル動物への遺伝子導入を試みている。

  12. Spatiotemporal knockout by an Adenovirus-Targeting System.

    Grant number:10044227  1998 - 2000

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A).

    KIYAMA Hiroshi, SEO-KIRYU Sumiko, KATO Hidemasa

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    Authorship:Coinvestigator(s) 

    The current experiment can be dissected down into two principles. First, it is essential for the recombinational events to occur only in the cells in question (ie, injured neurons in this case). Second, it is of large benefit to restrict the number of gene manipulation before observing the outcome. When a non-restrictive promoter is used to drive the expression from the adenoviruses, we were unable to target the Cre expression solely to the axonally-damaged neurons but would widely rearrange the neighboring cells. If the gene in question is closely related to the survival and/or maintenance of a cell, it is crucial to restrict the expression in a strict temporal and spatial manner. We then tried to gain some neuronal specificity within the adenovirus' promoter. As the size matters when recombining into the expression module of an adenovirus, we initially chose the relatively small SCG10/REST system. This has led to a relatively neuronal restrictive expression of a reporter gene. However, when tested with a Cre expressing system, the switch of the expressivity wasn't sharp enough to restrict the recombination to the damaged neurons. We next rested transgenic mice with a pan-neuronal specific promoter such as GAP-43 or nestin enhancer. These in turn showed some problems of their own. Nevertheless, it is of utmost importance to strictly regulate the gene expression and we hope to realize this through some refinements of these. We additionally obtained some interesting insights of the role of the target genes during nerve injury. c-Jun (especially its phosphorylation) is believed to play a part in the decision of cell fate during neuronal cell death. We do agree on this principle and therefore chose the molecule for the target of our silent-knockout (=the background mice for the conditional gene targeting). In an independent experiment, we showed that an additional member of the AP-1 family, Atf-3 might be as much crucial and cooperate with c-jun during death/survival decision. We are now Flaming to apply the current experiment paradigm for this molecule as well.

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Teaching Experience (On-campus) 10

  1. 人体形態学

    2020

  2. 基礎医学セミナー

    2020

  3. 組織学

    2020

  4. 肉眼解剖実習

    2020

  5. 組織学

    2014

  6. 人体形態学

    2014

  7. 人体形態学

    2014

  8. 人体形態学

    2012

  9. 解剖学実習

    2012

  10. 組織学

    2012

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Teaching Experience (Off-campus) 1

  1. 神経解剖学

    Osaka City University)