研究者詳細 - 廣明　秀一

論文 - 廣明　秀一

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Synthesis of selenomethylene-locked nucleic acids (SeLNA) nucleoside unit bearing an adenine base 査読有り国際誌

Moai, Y; Hiroaki, H; Obika, S; Kodama, T

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS 39 巻 ( 1-3 ) 頁： 131 - 140 2020年2月

Discovery of Cryoprotective Activity in Human Genome-Derived Intrinsically Disordered Proteins

Matsuo Naoki, Goda Natsuko, Shimizu Kana, Fukuchi Satoshi, Ota Motonori, Hiroaki Hidekazu

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19 巻 ( 2 ) 2018年2月

Common molecular pathogenesis of disease-related intrinsically disordered proteins revealed by NMR analysis. 査読有り

Shigemitsu Y, Hiroaki H

Journal of biochemistry 163 巻 ( 1 ) 頁： 11-18 2018年1月

Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast. 査読有り

Shimasaki T, Ohtsuka H, Naito C, Azuma K, Tenno T, Hiroaki H, Murakami H, Aiba H

Molecular genetics and genomics : MGG 292 巻 ( 2 ) 頁： 475-481 2017年4月

Benzene-Glycol Nucleic Acid (BGNA)-DNA Chimeras: Synthesis, Binding Properties, and Ability to Elicit Human RNase H Activity. 査読有り

Niwa, N., Shimizu, S., Maeda, Y., Hiroaki, H., Ueno, Y.

7 巻頁： 25378-25386 2017年1月

Nuclear magnetic resonance evidence for the dimer formation of beta amyloid peptide 1-42 in 1,1,1,3,3,3-hexafluoro-2-propanol. 査読有り

Shigemitsu Y, Iwaya N, Goda N, Matsuzaki M, Tenno T, Narita A, Hoshi M, Hiroaki H

Analytical biochemistry 498 巻頁： 59-67 2016年4月

Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly. 査読有り

112 巻 ( 32 ) 頁： E4465-74 2015年8月

用語解説「天然変性蛋白質」招待有り

廣明秀一

再生医療（日本再生医療学会雑誌） 14 巻 ( 3 ) 頁： 52-53 2015年8月

A Method for Systematic Assessment of Intrinsically Disordered Protein Regions by NMR. 査読有り

Goda N, Shimizu K, Kuwahara Y, Tenno T, Noguchi T, Ikegami T, Ota M, Hiroaki H

International journal of molecular sciences 16 巻 ( 7 ) 頁： 15743-60 2015年7月

Crystal structure of afadin PDZ domain-nectin-3 complex shows the structural plasticity of the ligand-binding site. 査読有り

Fujiwara Y, Goda N, Tamashiro T, Narita H, Satomura K, Tenno T, Nakagawa A, Oda M, Suzuki M, Sakisaka T, Takai Y, Hiroaki H

Protein science : a publication of the Protein Society 24 巻 ( 3 ) 頁： 376-85 2015年3月

An optimized Npro-based method for the expression and purification of intrinsically disordered proteins for an NMR study 査読有り

Natsuko Goda, Naoki Matsuo, Takeshi Tenno, Sonoko Ishino, Yoshizumi Ishino, Satoshi Fukuchi, Motonori Ota & Hidekazu Hiroaki*

ntrinsically Disordered Proteins 3 巻 ( 1 ) 頁： 1-6 2015年2月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Intrinsically disordered proteins (IDPs) are an emerging concept. IDPs have high flexibility in their polypeptide chains, lacking a stable 3-dimensional structure. Because of the difficulty in performing X-ray crystallography for IDPs, nuclear magnetic resonance (NMR) spectroscopy is the first choice for atomic-level investigation of their nature. Given that isotopically labeled IDP samples are necessary for NMR study, a robust and cost-effective protocol for bacterial expression and purification of IDP is also needed. We employed the Npro (EDDIE)-autoprotease fusion protein system. Although IDPs are believed to be readily degraded by endogenous proteases when expressed in Escherichia coli, Npro-fused IDPs showed excellent resistance to degradation. Seven IDPs of uncharacterized function sampled from the human genome as well as 3 constructs from IDP regions derived from human FancM and Thermococcus kodakarensis Hef were prepared. We improved the protocol of refolding of Npro (EDDIE) to use dialysis, which is convenient for subsequent purification using reversed-phase (RP) HPLC. The method is robust and widely applicable to any IDP sample, promoting the acquisition of experimental data for IDPs in a high-throughput manner.

DOI： 10.1080/21690707.2015.1011004

Decreased amyloidogenicity caused by mutational modulation of surface properties of the immunoglobulin light chain BRE variable domain. 査読有り

Kobayashi Y, Tsutsumi H, Abe T, Ikeda K, Tashiro Y, Unzai S, Kamikubo H, Kataoka M, Hiroaki H, Hamada D.

Biochemistry 53 巻 ( 31 ) 頁： 5162-5173 2014年8月

Multiple interactions of the intrinsically disordered region between the helicase and nuclease domains of the archaeal Hef protein. 査読有り

Ishino S, Yamagami T, Kitamura M, Kodera N, Mori T, Sugiyama S, Ando T, Goda N, Tenno T, Hiroaki H, Ishino Y.

J Biol Chem. 289 巻 ( 31 ) 頁： 21627-21639 2014年8月

IDEAL in 2014 illustrates interaction networks composed of intrinsically disordered proteins and their binding partners. 査読有り

Fukuchi S, Amemiya T, Sakamoto S, Nobe Y, Hosoda K, Kado Y, Murakami SD, Koike R, Hiroaki H, Ota M.

Nucleic Acids Res. 42 巻 ( Database issue ) 頁： D320-325 2014年1月

特集「タンパク質構造機能相関再考」~ NMRで見るタンパク質－低分子相互作用 (2013) 招待有り

廣明秀一

生化学 85 巻 ( 8 ) 頁： 679-686 2013年8月

Accidental Interaction between PDZ Domains and Diclofenac Revealed by NMR-Assisted Virtual Screening. 査読有り

Tenno T, Goda N, Umetsu Y, Ota M, Kinoshita K, Hiroaki H.

Molecules 18 巻 ( 8 ) 頁： 9567-9581 2013年8月

Recent applications of isotopic labeling for protein NMR in drug discovery. 招待有り査読有り

Hiroaki, H*

Expert Opinion on Drug Discovery 8 巻 ( 5 ) 頁： 523-536 2013年3月

Effect of Ca(2+) on the microtubule-severing enzyme p60-katanin. Insight into the substrate-dependent activation mechanism. 査読有り

Iwaya N, Akiyama K, Goda N, Tenno T, Fujiwara Y, Hamada D, Ikura T, Shirakawa M, Hiroaki H.

FEBS J. 279 巻 ( 7 ) 頁： 1339-1352. 2012年3月

IDEAL: Intrinsically Disordered proteins with Extensive Annotations and Literature. 査読有り

Fukuchi S, Sakamoto S, Nobe Y, Murakami SD, Amemiya T, Hosoda K, Koike R, Hiroaki H, Ota M.

Nucleic Acids Res. 40 巻頁： D507-511. 2011年11月

A simplified recipe for assigning amide NMR signals using combinatorial 14N amino acid inverse-labeling. 査読有り

Hiroaki H, Umetsu Y, Nabeshima Y, Hoshi M, Kohda D.

J Struct Funct Genomics. 12 巻 ( 3 ) 頁： 167-174 2011年8月

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