研究者詳細 - 廣明　秀一

論文 - 廣明　秀一

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A Cost-Effective Immobilization Method for MBP Fusion Proteins on Microtiter Plates Using a Gelatinized Starch-Agarose Mixture and Its Application for Convenient Protein-Protein Interaction Analysis 査読有り国際誌

Emoto, Y; Katayama, R; Hibino, E; Ishihara, S; Goda, N; Tenno, T; Kobashigawa, Y; Morioka, H; Hiroaki, H

METHODS AND PROTOCOLS 6 巻 ( 3 ) 2023年6月

Pharmacologic Comparison of High-Dose Hesperetin and Quercetin on MDCK II Cell Viability, Tight Junction Integrity, and Cell Shape 査読有り国際誌

Nakashima, M; Goda, N; Tenno, T; Kotake, A; Inotsume, Y; Amaya, M; Hiroaki, H

ANTIOXIDANTS 12 巻 ( 4 ) 2023年4月

Small-Molecule-Mediated Suppression of BMP Signaling by Selective Inhibition of BMP1-Dependent Chordin Cleavage 査読有り国際誌

Mizoguchi Takamasa, Mikami Shohei, Yatou Mari, Kondo Yui, Omaru Shuhei, Kuwabara Shuhei, Okura Wataru, Noda Syouta, Tenno Takeshi, Hiroaki Hidekazu, Itoh Motoyuki

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 24 巻 ( 5 ) 2023年3月

Distinct motifs in the E protein are required for SARS-CoV-2 virus particle formation and lysosomal deacidification in host cells 査読有り国際誌

Miura, K; Suzuki, Y; Ishida, K; Arakawa, M; Wu, H; Fujioka, Y; Emi, A; Maeda, K; Hamajima, R; Nakano, T; Tenno, T; Hiroaki, H; Morita, E

JOURNAL OF VIROLOGY 97 巻 ( 10 ) 頁： e0042623 2023年10月

Molecular mechanisms of amyloid-beta peptide fibril and oligomer formation: NMR-based challenges 招待有り査読有り国際誌

Hiroaki, H

BIOPHYSICS AND PHYSICOBIOLOGY 20 巻 ( 1 ) 頁： e200007 2023年

Relevance of Amorphous and Amyloid-Like Aggregates of the p53 Core Domain to Loss of its DNA-Binding Activity 査読有り国際誌

Hibino Emi, Tenno Takeshi, Hiroaki Hidekazu

FRONTIERS IN MOLECULAR BIOSCIENCES 9 巻頁： 869851 2022年4月

Direct inhibition of the first PDZ domain of ZO-1 by glycyrrhizin is a possible mechanism of tight junction opening of Caco-2 cells 査読有り国際誌

Hibino, E; Goda, N; Hisada, M; Tenno, T; Hiroaki, H

FOOD & FUNCTION 13 巻 ( 4 ) 頁： 1953 - 1964 2022年2月

Potential of rescue and reactivation of tumor suppressor p53 for cancer therapy 招待有り査読有り国際誌

Hibino, E; Hiroaki, H

BIOPHYSICAL REVIEWS 14 巻 ( 1 ) 頁： 267 - 275 2022年2月

A cryptic phosphate-binding pocket on the SPFH domain of human stomatin that regulates a novel fibril-like self-assembly 査読有り国際誌

Kataoka, K; Suzuki, S; Tenno, T; Goda, N; Hibino, E; Oshima, A; Hiroaki, H

CURRENT RESEARCH IN STRUCTURAL BIOLOGY 4 巻頁： 158 - 166 2022年

Cryoprotective activities of FK20, a human genome-derived intrinsically disordered peptide against cryosensitive enzymes without a stereospecific molecular interaction 査読有り国際誌

Naoki Matsuo, Natsuko Goda, Takeshi Tenno, and Hidekazu Hiroaki

PeerJ Physical Chemistry 3 巻頁： e20 2021年12月

1Structural and Functional Analysis of Glycyrrhizin Bound to the Scaffold Protein ZO1-PDZ1 of Tight Junctions 査読有り国際誌

Hibino, E; Hisada, M; Goda, N; Tenno, T; Hiroaki, H

PROTEIN SCIENCE 30 巻頁： 119 - 119 2021年10月

NMR-Guided Repositioning of Non-Steroidal Anti-Inflammatory Drugs into Tight Junction Modulators 査読有り国際誌

Tenno, T; Kataoka, K; Goda, N; Hiroaki, H

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 22 巻 ( 5 ) 2021年3月

High dose of baicalin or baicalein can reduce tight junction integrity by partly targeting the first PDZ domain of zonula occludens-1 (ZO-1) 査読有り

Hisada, M; Hiranuma, M; Nakashima, M; Goda, N; Tenno, T; Hiroaki, H

EUROPEAN JOURNAL OF PHARMACOLOGY 887 巻頁： 173436 2020年11月

Opposing Effect of Naringenin and Quercetin on the Junctional Compartment of MDCK II Cells to Modulate the Tight Junction 査読有り国際誌

Nakashima, M; Hisada, M; Goda, N; Tenno, T; Kotake, A; Inotsume, Y; Kameoka, I; Hiroaki, H

NUTRIENTS 12 巻 ( 11 ) 2020年11月

Principal component analysis of data from NMR titration experiment of uniformly N-15 labeled amyloid beta (1-42) peptide with osmolytes and phenolic compounds 査読有り

Iwaya Naoko, Goda Natsuko, Matsuzaki Mizuki, Narita Akihiro, Shigemitsu Yoshiki, Tenno Takeshi, Abe Yoshito, Hoshi Minako, Hiroaki Hidekazu

ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS 690 巻頁： 108446 2020年9月

Presence of intrinsically disordered proteins can inhibit the nucleation phase of amyloid fibril formation of A beta(1-42) in amino acid sequence independent manner 査読有り

Ikeda Koki, Suzuki Shota, Shigemitsu Yoshiki, Tenno Takeshi, Goda Natsuko, Oshima Atsunori, Hiroaki Hidekazu

SCIENTIFIC REPORTS 10 巻 ( 1 ) 頁： 12334 2020年7月

Spatial Overlap of Claudin- and Phosphatidylinositol Phosphate-Binding Sites on the First PDZ Domain of Zonula Occludens 1 Studied by NMR 査読有り

Hiroaki Hidekazu, Satomura Kaori, Goda Natsuko, Nakakura Yukako, Hiranuma Minami, Tenno Takeshi, Hamada Daizo, Ikegami Takahisa

MOLECULES 23 巻 ( 10 ) 2018年10月

Using H-1(N) amide temperature coefficients to define intrinsically disordered regions: An alternative NMR method 査読有り

Okazaki Hiroki, Matsuo Naoki, Tenno Takeshi, Goda Natsuko, Shigemitsu Yoshiki, Ota Motonori, Hiroaki Hidekazu

PROTEIN SCIENCE 27 巻 ( 10 ) 頁： 1821 - 1830 2018年10月

Discovery of Potent Disheveled/Dvl Inhibitors Using Virtual Screening Optimized With NMR-Based Docking Performance Index 招待有り査読有り国際誌

Hori, K; Ajioka, K; Goda, N; Shindo, A; Takagishi, M; Tenno, T; Hiroaki, H

FRONTIERS IN PHARMACOLOGY 9 巻頁： 983 2018年9月

Analysis and comparison of amorphous& amyloid aggregates of the tumor suppressor p53

Hibino, E; Tenno, T; Hiroaki, H

BIOPHYSICAL JOURNAL 122 巻 ( 3 ) 頁： 351A - 351A 2023年2月

Synthesis of selenomethylene-locked nucleic acids (SeLNA) nucleoside unit bearing an adenine base 査読有り国際誌

Moai, Y; Hiroaki, H; Obika, S; Kodama, T

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS 39 巻 ( 1-3 ) 頁： 131 - 140 2020年2月

Discovery of Cryoprotective Activity in Human Genome-Derived Intrinsically Disordered Proteins

Matsuo Naoki, Goda Natsuko, Shimizu Kana, Fukuchi Satoshi, Ota Motonori, Hiroaki Hidekazu

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES 19 巻 ( 2 ) 2018年2月

Common molecular pathogenesis of disease-related intrinsically disordered proteins revealed by NMR analysis. 査読有り

Shigemitsu Y, Hiroaki H

Journal of biochemistry 163 巻 ( 1 ) 頁： 11-18 2018年1月

Ecl1 is a zinc-binding protein involved in the zinc-limitation-dependent extension of chronological life span in fission yeast. 査読有り

Shimasaki T, Ohtsuka H, Naito C, Azuma K, Tenno T, Hiroaki H, Murakami H, Aiba H

Molecular genetics and genomics : MGG 292 巻 ( 2 ) 頁： 475-481 2017年4月

Benzene-Glycol Nucleic Acid (BGNA)-DNA Chimeras: Synthesis, Binding Properties, and Ability to Elicit Human RNase H Activity. 査読有り

Niwa, N., Shimizu, S., Maeda, Y., Hiroaki, H., Ueno, Y.

7 巻頁： 25378-25386 2017年1月

Nuclear magnetic resonance evidence for the dimer formation of beta amyloid peptide 1-42 in 1,1,1,3,3,3-hexafluoro-2-propanol. 査読有り

Shigemitsu Y, Iwaya N, Goda N, Matsuzaki M, Tenno T, Narita A, Hoshi M, Hiroaki H

Analytical biochemistry 498 巻頁： 59-67 2016年4月

Na, K-ATPase α3 is a death target of Alzheimer patient amyloid-β assembly. 査読有り

112 巻 ( 32 ) 頁： E4465-74 2015年8月

用語解説「天然変性蛋白質」招待有り

廣明秀一

再生医療（日本再生医療学会雑誌） 14 巻 ( 3 ) 頁： 52-53 2015年8月

A Method for Systematic Assessment of Intrinsically Disordered Protein Regions by NMR. 査読有り

Goda N, Shimizu K, Kuwahara Y, Tenno T, Noguchi T, Ikegami T, Ota M, Hiroaki H

International journal of molecular sciences 16 巻 ( 7 ) 頁： 15743-60 2015年7月

Crystal structure of afadin PDZ domain-nectin-3 complex shows the structural plasticity of the ligand-binding site. 査読有り

Fujiwara Y, Goda N, Tamashiro T, Narita H, Satomura K, Tenno T, Nakagawa A, Oda M, Suzuki M, Sakisaka T, Takai Y, Hiroaki H

Protein science : a publication of the Protein Society 24 巻 ( 3 ) 頁： 376-85 2015年3月

An optimized Npro-based method for the expression and purification of intrinsically disordered proteins for an NMR study 査読有り

Natsuko Goda, Naoki Matsuo, Takeshi Tenno, Sonoko Ishino, Yoshizumi Ishino, Satoshi Fukuchi, Motonori Ota & Hidekazu Hiroaki*

ntrinsically Disordered Proteins 3 巻 ( 1 ) 頁： 1-6 2015年2月

担当区分：筆頭著者記述言語：英語掲載種別：研究論文（学術雑誌）

Intrinsically disordered proteins (IDPs) are an emerging concept. IDPs have high flexibility in their polypeptide chains, lacking a stable 3-dimensional structure. Because of the difficulty in performing X-ray crystallography for IDPs, nuclear magnetic resonance (NMR) spectroscopy is the first choice for atomic-level investigation of their nature. Given that isotopically labeled IDP samples are necessary for NMR study, a robust and cost-effective protocol for bacterial expression and purification of IDP is also needed. We employed the Npro (EDDIE)-autoprotease fusion protein system. Although IDPs are believed to be readily degraded by endogenous proteases when expressed in Escherichia coli, Npro-fused IDPs showed excellent resistance to degradation. Seven IDPs of uncharacterized function sampled from the human genome as well as 3 constructs from IDP regions derived from human FancM and Thermococcus kodakarensis Hef were prepared. We improved the protocol of refolding of Npro (EDDIE) to use dialysis, which is convenient for subsequent purification using reversed-phase (RP) HPLC. The method is robust and widely applicable to any IDP sample, promoting the acquisition of experimental data for IDPs in a high-throughput manner.

DOI： 10.1080/21690707.2015.1011004

Decreased amyloidogenicity caused by mutational modulation of surface properties of the immunoglobulin light chain BRE variable domain. 査読有り

Kobayashi Y, Tsutsumi H, Abe T, Ikeda K, Tashiro Y, Unzai S, Kamikubo H, Kataoka M, Hiroaki H, Hamada D.

Biochemistry 53 巻 ( 31 ) 頁： 5162-5173 2014年8月

Multiple interactions of the intrinsically disordered region between the helicase and nuclease domains of the archaeal Hef protein. 査読有り

Ishino S, Yamagami T, Kitamura M, Kodera N, Mori T, Sugiyama S, Ando T, Goda N, Tenno T, Hiroaki H, Ishino Y.

J Biol Chem. 289 巻 ( 31 ) 頁： 21627-21639 2014年8月

IDEAL in 2014 illustrates interaction networks composed of intrinsically disordered proteins and their binding partners. 査読有り

Fukuchi S, Amemiya T, Sakamoto S, Nobe Y, Hosoda K, Kado Y, Murakami SD, Koike R, Hiroaki H, Ota M.

Nucleic Acids Res. 42 巻 ( Database issue ) 頁： D320-325 2014年1月

特集「タンパク質構造機能相関再考」~ NMRで見るタンパク質－低分子相互作用 (2013) 招待有り

廣明秀一

生化学 85 巻 ( 8 ) 頁： 679-686 2013年8月

Accidental Interaction between PDZ Domains and Diclofenac Revealed by NMR-Assisted Virtual Screening. 査読有り

Tenno T, Goda N, Umetsu Y, Ota M, Kinoshita K, Hiroaki H.

Molecules 18 巻 ( 8 ) 頁： 9567-9581 2013年8月

Recent applications of isotopic labeling for protein NMR in drug discovery. 招待有り査読有り

Hiroaki, H*

Expert Opinion on Drug Discovery 8 巻 ( 5 ) 頁： 523-536 2013年3月

Effect of Ca(2+) on the microtubule-severing enzyme p60-katanin. Insight into the substrate-dependent activation mechanism. 査読有り

Iwaya N, Akiyama K, Goda N, Tenno T, Fujiwara Y, Hamada D, Ikura T, Shirakawa M, Hiroaki H.

FEBS J. 279 巻 ( 7 ) 頁： 1339-1352. 2012年3月

IDEAL: Intrinsically Disordered proteins with Extensive Annotations and Literature. 査読有り

Fukuchi S, Sakamoto S, Nobe Y, Murakami SD, Amemiya T, Hosoda K, Koike R, Hiroaki H, Ota M.

Nucleic Acids Res. 40 巻頁： D507-511. 2011年11月

A simplified recipe for assigning amide NMR signals using combinatorial 14N amino acid inverse-labeling. 査読有り

Hiroaki H, Umetsu Y, Nabeshima Y, Hoshi M, Kohda D.

J Struct Funct Genomics. 12 巻 ( 3 ) 頁： 167-174 2011年8月

Structure and Function of the N-terminal Nucleolin Binding Domain of Nuclear Valosin-containing Protein-like 2 (NVL2) Harboring a Nucleolar Localization Signal. 査読有り

Fujiwara, Y., Fujiwara, K., Goda, N., Iwaya, N., Tenno, T., Shirakawa, M., and Hiroaki, H.

J Biol Chem. 286 巻 ( 24 ) 頁： 21732-21741. 2011年6月

(1)H, (13)C, and (15)N resonance assignment of the SPFH domain of human stomatin. 査読有り

Tsuruta, T., Goda, N., Umetsu, Y., Iwaya, N., Kuwahara, Y., and Hiroaki, H.

Biomol NMR Assign. 6 巻 ( 1 ) 頁： 23-25. 2011年6月

SH3YL1 regulates dorsal ruffle formation by a novel phosphoinositide-binding domain. 査読有り

Hasegawa, J., Tokuda, E., Tenno, T., Tsujita, K., Sawai, H., Hiroaki, H., Takenawa, T., and Itoh, T.

J Cell Biol. 193 巻 ( 5 ) 頁： 901-916. 2011年5月

Structural difference of vasoactive intestinal peptide in two distinct membrane-mimicking environments. 査読有り

Umetsu, Y., Tenno, T., Goda, N., Shirakawa, M., Ikegami, T., and Hiroaki, H.

Biochim Biophys Acta. 1814 巻 ( 5 ) 頁： 724-730. 2011年5月

(1)H, (13)C, and (15)N resonance assignment of the first PDZ domain of mouse ZO-1. 査読有り

Umetsu, Y., Goda, N., Taniguchi, R., Satomura, K., Ikegami, T., Furuse, M., and Hiroaki, H.

Biomol NMR Assign. 5 巻 ( 2 ) 頁： 207-210. 2011年3月

SAHG, a comprehensive database of predicted structures of all human proteins. 査読有り

Motono, C., Nakata, J., Koike, R., Shimizu, K., Shirota, M., Amemiya, T., Tomii, K., Nagano, N., Sakaya, N., Misoo, K., Sato, M., Kidera, A., Hiroaki, H., Shirai, T., Kinoshita, K., Noguchi, T., and Ota, M.

Nucleic Acids Res. 39 巻頁： D487-493. 2011年1月

Crystal structure of a PFU-PUL domain pair of Saccharomyces cerevisiae Doa1/Ufd3. 査読有り

Nishimasu, R., Komori, H., Higuchi, Y., Nishimasu, H., and Hiroaki, H.

Kobe J Med Sci. 56 巻 ( 3 ) 頁： E125-139. 2010年10月

1H, 13C, and 15N resonance assignment of the TIR domain of human MyD88. 査読有り

Ohnishi, H., Tochio, H., Kato, Z., Kimura, T., Hiroaki, H., Kondo, N., and Shirakawa, M.

Biomol NMR Assign. 4 巻 ( 2 ) 頁： 123-125. 2010年10月

A common substrate recognition mode conserved between katanin p60 and VPS4 governs microtubule severing and membrane skeleton reorganization. 査読有り

Iwaya, N., Kuwahara, Y., Fujiwara, Y., Goda, N., Tenno, T., Akiyama, K., Mase, S., Tochio, H., Ikegami, T., Shirakawa, M., and Hiroaki, H.

J Biol Chem. 285 巻 ( 22 ) 頁： 16822-16829. 2010年5月

Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins. 査読有り

Jee, J., Mizuno, T., Kamada, K., Tochio, H., Chiba, Y., Yanagi, K., Yasuda, G., Hiroaki, H., Hanaoka, F., and Shirakawa, M.

J Biol Chem. 285 巻 ( 21 ) 頁： 15931-15940. 2010年5月

Unusual thermal disassembly of the SPFH domain oligomer from Pyrococcus horikoshii. 査読有り

Kuwahara, Y., Unzai, S., Nagata, T., Hiroaki, Y., Yokoyama, H., Matsui, I., Ikegami, T., Fujiyoshi, Y., and Hiroaki, H.

Biophys J. 97 巻 ( 7 ) 頁： 2034-2043. 2009年10月

Structural basis for the multiple interactions of the MyD88 TIR domain in TLR4 signaling. 査読有り

Ohnishi, H., Tochio, H., Kato, Z., Orii, K. E., Li, A., Kimura, T., Hiroaki, H., Kondo, N., and Shirakawa, M.

Proc Natl Acad Sci U S A. 106 巻 ( 25 ) 頁： 10260-10265. 2009年6月

High-resolution multi-dimensional NMR spectroscopy of proteins in human cells. 査読有り

Inomata, K., Ohno, A., Tochio, H., Isogai, S., Tenno, T., Nakase, I., Takeuchi, T., Futaki, S., Ito, Y., Hiroaki, H., and Shirakawa, M.

Nature. 458 巻 ( 7234 ) 頁： 106-109. 2009年3月

The solution structure of the C-terminal domain of NfeD reveals a novel membrane-anchored OB-fold. 査読有り

Kuwahara, Y., Ohno, A., Morii, T., Yokoyama, H., Matsui, I., Tochio, H., Shirakawa, M., and Hiroaki, H.

Protein Sci. 17 巻 ( 11 ) 頁： 1915-1924. 2008年11月

Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5. 査読有り

Goda, N., Tenno, T., Inomata, K., Shirakawa, M., Tanaka, T., and Hiroaki, H.

Exp Cell Res. 314 巻 ( 13 ) 頁： 2352-2361. 2008年8月

DNA damage-induced ubiquitylation of RFC2 subunit of replication factor C complex. 査読有り

Tomida, J., Masuda, Y., Hiroaki, H., Ishikawa, T., Song, I., Tsurimoto, T., Tateishi, S., Shiomi, T., Kamei, Y., Kim, J., Kamiya, K., Vaziri, C., Ohmori, H., and Todo, T.

J Biol Chem. 283 巻 ( 14 ) 頁： 9071-9079. 2008年4月

Kinetic and Thermodynamic Evidence for Flipping of a Methyl-CpG Binding Domain on Methylated DNA. 査読有り

Inomata, K., Ohki, I., Tochio, H., Fujiwara, K., Hiroaki, H., and Shirakawa, M.

Biochemistry. 47 巻 ( 10 ) 頁： 3266-3271. 2008年3月

Fluoroscopic assessment of protein leakage during Xenopus oocytes in-cell NMR experiment by co-injected EGFP. 査読有り

Sakai, T., Tochio, H., Inomata, K., Sasaki, Y., Tenno, T., Tanaka, T., Kokubo, T., Hiroaki, H., and Shirakawa, M.

Anal Biochem. 371 巻 ( 2 ) 頁： 247-249. 2007年12月

LBT/PTD dual tagged vector for purification, cellular protein delivery and visualization in living cells. 査読有り

Goda, N., Tenno, T., Inomata, K., Iwaya, N., Sasaki, Y., Shirakawa, M., and Hiroaki, H.

Biochim Biophys Acta. 1773 巻 ( 2 ) 頁： 141-146. 2007年2月

Fine-tuning of protein domain boundary by minimizing potential coiled coil regions. 査読有り

Iwaya, N., Goda, N., Unzai, S., Fujiwara, K., Tanaka, T., Tomii, K., Tochio, H., Shirakawa, M., and Hiroaki, H.

J Biomol NMR. 37 巻 ( 1 ) 頁： 53-63. 2007年1月

The common phospholipid-binding activity of the N-terminal domains of PEX1 and VCP/p97. 査読有り

Shiozawa, K., Goda, N., Shimizu, T., Mizuguchi, K., Kondo, N., Shimozawa, N., Shirakawa, M., and Hiroaki, H.

FEBS J. 273 巻 ( 21 ) 頁： 4959-4571. 2006年11月

In-cell NMR spectroscopy of proteins inside Xenopus laevis oocytes. 査読有り

Sakai, T., Tochio, H., Tenno, T., Ito, Y., Kokubo, T., Hiroaki, H., and Shirakawa, M.

J Biomol NMR. 36 巻 ( 3 ) 頁： 179-188. 2006年11月

Crystal structure of SUMO-3-modified thymine-DNA glycosylase. 査読有り

Baba, D., Maita, N., Jee, J. G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H., and Shirakawa, M.

J Mol Biol. 359 巻 ( 1 ) 頁： 137-147. 2006年5月

Optimization of size complementary of the aromatic side chain in the hydrophobic core of the designed coiled-coil. 査読有り

Sakurai, Y., Mizuno, T., Hiroaki, H., Oku, J., and Tanaka, T.

J Pept Res. 66 巻 ( 6 ) 頁： 387-394. 2005年12月

Crystal structure of thymine DNA glycosylase conjugated to SUMO-1. 査読有り

Baba, D., Maita, N., Jee, J. G., Uchimura, Y., Saitoh, H., Sugasawa, K., Hanaoka, F., Tochio, H., Hiroaki, H., and Shirakawa, M.

Nature. 435 巻 ( 7044 ) 頁： 979-982. 2005年10月

High thermal stability imparted by a designed tandem Arg-Trp stretch in an alpha-helical coiled coil. 査読有り

Sakurai, Y., Mizuno, T., Hiroaki, H., Gohda, K., Oku, J., and Tanaka, T.

Angew Chem Int Ed Engl. 44 巻 ( 38 ) 頁： 6180-6183. 2005年9月

Structural characterization of the MIT domain from human Vps4b. 査読有り

Takasu, H., Jee, J. G., Ohno, A., Goda, N., Fujiwara, K., Tochio, H., Shirakawa, M., and Hiroaki, H.

Biochem Biophys Res Commun. 334 巻 ( 2 ) 頁： 460-465. 2005年8月

Structure of the UBA domain of Dsk2p in complex with ubiquitin molecular determinants for ubiquitin recognition. 査読有り

Ohno, A., Jee, J., Fujiwara, K., Tenno, T., Goda, N., Tochio, H., Kobayashi, H., Hiroaki, H., and Shirakawa, M.

Structure (Camb). 13 巻 ( 4 ) 頁： 521-532. 2005年4月

[High-throughput construction of expression vector for protein domain analysis].

Tenno, T., Goda, N., and Hiroaki, H.

Tanpakushitsu Kakusan Koso. 49 巻 ( 16 ) 頁： 2587-2594. 2004年12月

Structure of the N-terminal domain of PEX1 AAA-ATPase. Characterization of a putative adaptor-binding domain. 査読有り

Shiozawa, K., Maita, N., Tomii, K., Seto, A., Goda, N., Akiyama, Y., Shimizu, T., Shirakawa, M., and Hiroaki, H.

J Biol Chem. 279 巻 ( 48 ) 頁： 50060-50068. 2004年11月

Crystallographic characterization of the N-terminal domain of PEX1. 査読有り

Shiozawa, K., Maita, N., Tomii, K., Seto, A., Goda, N., Akiyama, Y., Shimizu, T., Shirakawa, M., and Hiroaki, H.

Acta Crystallogr D Biol Crystallogr. 60 巻 ( 11 ) 頁： 2098-2099. 2004年11月

Two-Metal Ion, Ni(II) and Cu(II), Binding alpha-Helical Coiled Coil Peptide. 査読有り

Tanaka, T., Mizuno, T., Fukui, S., Hiroaki, H., Oku, J. I., Kanaori, K., Tajima, K., and Shirakawa, M.

J Am Chem Soc. 126 巻 ( 43 ) 頁： 14023-14028. 2004年10月

Structural basis for distinct roles of Lys63- and Lys48-linked polyubiquitin chains. 査読有り

Tenno, T., Fujiwara, K., Tochio, H., Iwai, K., Morita, E. H., Hayashi, H., Murata, S., Hiroaki, H., Sato, M., Tanaka, K., and Shirakawa, M.

Genes Cells. 9 巻 ( 10 ) 頁： 865-875. 2004年10月

High-throughput construction method for expression vector of peptides for NMR study suited for isotopic labeling. 査読有り

Tenno, T., Goda, N., Tateishi, Y., Tochio, H., Mishima, M., Hayashi, H., Shirakawa, M., and Hiroaki, H.

Protein Eng Des Sel. 17 巻 ( 4 ) 頁： 305-314. 2004年4月

The PRESAT-vector: asymmetric T-vector for high-throughput screening of soluble protein domains for structural proteomics. 査読有り

Goda, N., Tenno, T., Takasu, H., Hiroaki, H., and Shirakawa, M.

Protein Sci. 13 巻 ( 3 ) 頁： 652-658. 2004年3月

Structure of the ubiquitin-interacting motif of S5a bound to the ubiquitin-like domain of HR23B. 査読有り

Fujiwara, K., Tenno, T., Sugasawa, K., Jee, J. G., Ohki, I., Kojima, C., Tochio, H., Hiroaki, H., Hanaoka, F., and Shirakawa, M.

J Biol Chem. 279 巻 ( 6 ) 頁： 4760-4767. 2004年2月

Thermodynamic study of hybridization properties of heterochiral nucleic acids. 査読有り

Urata, H., Shimizu, H., Hiroaki, H., Kohda, D., and Akagi, M.

Biochem Biophys Res Commun. 309 巻 ( 1 ) 頁： 79-83. 2003年9月

1H, 13C and 15N backbone resonance assignments of the N-terminal domain of Drosophila GCM protein. 査読有り

Shimizu, M., Hiroaki, H., Kohda, D., Morita, E. H., Hotta, S., and Morikawa, K.

J Biomol NMR. 26 巻 ( 3 ) 頁： 277-278. 2003年7月

Phosphorylation of p47phox directs phox homology domain from SH3 domain toward phosphoinositides, leading to phagocyte NADPH oxidase activation. 査読有り

Ago, T., Kuribayashi, F., Hiroaki, H., Takeya, R., Ito, T., Kohda, D., and Sumimoto, H.

Proc Natl Acad Sci U S A. 100 巻 ( 8 ) 頁： 4474-4479. 2003年4月

NMR and ICP spectroscopic analysis of the DNA-binding domain of the Drosophila GCM protein reveals a novel Zn2+ -binding motif. 査読有り

Shimizu, M., Hiroaki, H., Kohda, D., Hosoya, T., Akiyama-Oda, Y., Hotta, Y., Morita, E. H., and Morikawa, K.

Protein Eng. 16 巻 ( 4 ) 頁： 247-254. 2003年4月

[Membrane associating protein domains: a new paradigm of phosphoinositide binding domains].

Hiroaki, H.

Tanpakushitsu Kakusan Koso. 46 巻 ( 13 ) 頁： 1936-1942. 2001年10月

The PX domain as a novel phosphoinositide- binding module. 査読有り

Ago, T., Takeya, R., Hiroaki, H., Kuribayashi, F., Ito, T., Kohda, D., and Sumimoto, H.

Biochem Biophys Res Commun. 287 巻 ( 3 ) 頁： 733-738. 2001年9月

Solution structure of the PX domain, a target of the SH3 domain. 査読有り

Hiroaki, H., Ago, T., Ito, T., Sumimoto, H., and Kohda, D.

Nat Struct Biol. 8 巻 ( 6 ) 頁： 526-530. 2001年6月

Characterization of DNA, RNA and DNA/RNA duplexes containing an L-nucleotide.

Urata, H., Shimizu, H., Hiroaki, H., Kohda, D., and Akagi, M.

Nucleic Acids Res Suppl. 1 巻頁： 243-244. 2001年

Design of a Heterotrimeric alpha -Helical Bundle by Hydrophobic Core Engineering. 査読有り

Kashiwada, A., Hiroaki, H., Kohda, D., Nango, M., and Tanaka, T.

J Am Chem Soc. 122 巻 ( 2 ) 頁： 212 -215. 2000年12月

Soft metal ions, Cd(II) and Hg(II), induce triple-stranded alpha-helical assembly and folding of a de novo designed peptide in their trigonal geometries. 査読有り

Li, X., Suzuki, K., Kanaori, K., Tajima, K., Kashiwada, A., Hiroaki, H., Kohda, D., and Tanaka, T.

Protein Sci. 9 巻 ( 7 ) 頁： 1327-1333. 2000年7月

A'-form RNA double helix in the single crystal structure of r(UGAGCUUCGGCUC). 査読有り

Tanaka, Y., Fujii, S., Hiroaki, H., Sakata, T., Tanaka, T., Uesugi, S., Tomita, K., and Kyogoku, Y.

Nucleic Acids Res. 27 巻 ( 4 ) 頁： 949-955. 1999年2月

Influence of various nucleotides on the in situ crystallization of Ca2+-ATPase. 査読有り

Hiroaki, Y., Mitsuoka, K., Cheng, Y., Hiroaki, H., and Fujiyoshi, Y.

Biochim Biophys Acta. 1415 巻 ( 2 ) 頁： 361-368. 1999年1月

Metal Ion Induced Self-Assembly of a Designed Peptide into a Triple-Stranded a Helical Bundle: A Novel Metal Binding Site in the Hydrophobic Core. 査読有り

Suzuki, K., Hiroaki, H., Kohda, D., Nakamura, H., and Tanaka, T.

J Am Chem Soc. 120 巻 ( 50 ) 頁： 13008 -13015. 1998年12月

An isoleucine zipper peptide forms a native-like triple stranded coiled coil in solution. 査読有り

Suzuki, K., Hiroaki, H., Kohda, D., and Tanaka, T.

Protein Eng. 11 巻 ( 11 ) 頁： 1051-1055. 1998年11月

Determination of the solution structure of the SH3 domain of human p56 Lck tyrosine kinase. 査読有り

Hiroaki, H., Klaus, W., and Senn, H.

J Biomol NMR. 8 巻 ( 2 ) 頁： 105-122. 1996年9月

Cloning of boar SPMI gene which is expressed specifically in seminal vesicle and codes for a sperm motility inhibitor protein. 査読有り

Iwamoto, T., Hiroaki, H., Furuichi, Y., Wada, K., Satoh, M., Satoh, M., Osada, T., and Gagnon, C.

FEBS Lett. 368 巻 ( 3 ) 頁： 420-424. 1995年7月

Spectroscopic Characterization of the Decadeoxynucleotide Duplex Modified with Dichloroethylene-diaminoplatinum(II). 査読有り

Urata, H., Urata, M., Akagi, M., Hiroaki, H., and Uesugi, S.

Nucleosides & Nucleotides. 13 巻頁： 1259-1269. 1994年7月

Properties Of A Hammerhead-Type Rna Enzyme-System That Consists Of 3 Rna Oligomer Strands. 査読有り

Odai, O., Hiroaki, H., Tanaka, T., and Uesugi, S.

Nucleosides & Nucleotides. 13 巻頁： 1569-1579. 1994年7月

Properties of hammerhead-type RNA enzyme derivatives which contain a G-to-I replacement in the loop region.

Uesugi, S., Kodama, H., Hiroaki, H., and Odai, O.

Nucleic Acids Symp Ser. 27 巻頁： 13-14. 1992年

Conformational features of the four successive non-Watson-Crick base pairs in RNA duplex.

Fujii, S., Tanaka, Y., Uesugi, S., Tanaka, T., Sakata, T., and Hiroaki, H.

Nucleic Acids Symp Ser. 27 巻頁： 63-64. 1992年

Interaction of bleomycin with deoxyribonucleic acid oligomer: proton nuclear magnetic resonance titration study using novel bleomycin complexes with Ni2+ and VO3+. 査読有り

Hiroaki, H., Nakayama, T., Ikehara, M., and Uesugi, S.

Chem Pharm Bull (Tokyo). 39 巻 ( 11 ) 頁： 2780-2786. 1991年11月

Conformation of 2'GMP bound to a mutant ribonuclease T1 (Y45W) determined by X-ray diffraction and NMR methods. 査読有り

Itoh, T., Tomita, K., Hakoshima, T., Hiroaki, H., Uesugi, S., Nishikawa, S., Amisaki, T., Morioka, H., Ohtsuka, E., and Ikehara, M.

J Biochem (Tokyo). 110 巻 ( 5 ) 頁： 677-680. 1991年11月

The molecular recognition mechanism of DNA by bleomycin.

Hiroaki, H., Uesugi, S., and Fujii, S.

Nucleic Acids Symp Ser. 25 巻頁： 147-148. 1991年

Molecular structure of extraordinarily stable RNA: model building and X-ray analysis.

Fujii, S., Tanaka, Y., Tomita, K., Sakata, T., Hiroaki, H., Tanaka, T., and Uesugi, S.

Nucleic Acids Symp Ser. 25 巻頁： 181-182. 1991年

Synthesis and NMR study of ribo-oligonucleotides forming a hammerhead-type RNA enzyme system. 査読有り

Odai, O., Kodama, H., Hiroaki, H., Sakata, T., Tanaka, T., and Uesugi, S.

Nucleic Acids Res. 18 巻 ( 20 ) 頁： 5955-5960. 1990年10月

Studies on the structure and stabilizing factor of the CUUCGG hairpin RNA using chemically synthesized oligonucleotides. 査読有り

Sakata, T., Hiroaki, H., Oda, Y., Tanaka, T., Ikehara, M., and Uesugi, S.

Nucleic Acids Res. 18 巻 ( 13 ) 頁： 3831-3839. 1990年7月

The role of a conserved guanosine residue in the hammerhead-type RNA enzyme. 査読有り

Odai, O., Hiroaki, H., Sakata, T., Tanaka, T., and Uesugi, S.

FEBS Lett. 267 巻 ( 1 ) 頁： 150-152. 1990年7月

Conformation and properties of hammerhead-type RNA enzymes.

Uesugi, S., Odai, O., Hiroaki, H., Kodama, H., Sakata, T., and Tanaka, T.

Nucleic Acids Symp Ser. 22 巻頁： 41-42. 1990年

Base recognition mechanism of bleomycin: solution structure of d(GGGGAGCTCCCC)2 based on 1H-NMR and restrained molecular dynamics.

Hiroaki, H., Ebata, T., Uesugi, S., and Fujii, S.

Nucleic Acids Symp Ser. 22 巻頁： 53-54. 1990年

Identification of DNA degradation products by antitumor antibiotic, esperamicin.

Ebata, T., Hiroaki, H., Uesugi, S., and Sugiura, Y.

Nucleic Acids Symp Ser. 22 巻頁： 19-20. 1990年

A 2-deoxyribonolactone-containing nucleotide: isolation and characterization of the alkali-sensitive photoproduct of the trideoxyribonucleotide d(ApCpA). 査読有り

Urata, H., Yamamoto, K., Akagi, M., Hiroaki, H., and Uesugi, S.

Biochemistry. 28 巻 ( 25 ) 頁： 9566-9569. 1989年12月

Novel 1H-31P heteronuclear coherence transfer spectroscopy based on spin locking MLEV-17 and its application to signal assignment for a short DNA fragment. 査読有り

Hiroaki, H., and Uesugi, S.

FEBS Lett. 244 巻 ( 1 ) 頁： 43-46. 1989年2月

Synthesis and properties of RNA with catalytic activity.

Odai, O., Sakata, T., Orita, M., Hiroaki, H., Uesugi, S., and Tanaka, T.

Nucleic Acids Symp Ser. 21 巻頁： 105-106. 1989年

NMR studies on DNA hairpin structures with a five nucleotide loop.

Uesugi, S., Oda, Y., Hiroaki, H., and Ikehara, M.

Nucleic Acids Symp Ser. 21 巻頁： 65-66. 1989年

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