Updated on 2024/06/12

写真a

 
HORI Katsutoshi
 
Organization
Graduate School of Engineering Biomolecular Engineering 2 Professor
Graduate School
Graduate School of Engineering
Undergraduate School
School of Engineering Chemistry and Biotechnology
Title
Professor
Contact information
メールアドレス

Degree 1

  1. 博士(工学) ( 1995.4   東京工業大学 ) 

Research Interests 1

  1. Microorganism, Biofilm, Environmental biotechnology, Protein, DNA, Synthetic biology, Wastewater treatment, Bioremediation, Microbial adhesion

Research Areas 1

  1. Manufacturing Technology (Mechanical Engineering, Electrical and Electronic Engineering, Chemical Engineering) / Biofunction and bioprocess engineering

Current Research Project and SDGs 2

  1. バイオによる排水処理

  2. バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

Education 3

  1. 東京工業大学大学院   総合理工学研究科

    - 1995.4

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    Country: Japan

  2. 東京工業大学大学院   総合理工学研究科

    - 1991.3

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    Country: Japan

  3. 東京理科大学   理工学部

    - 1988.3

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    Country: Japan

Professional Memberships 6

  1. 生物工学会   理事、代議員、英文誌編集委員、中部支部長

  2. 日本化学会   バイオテクノロジー部会長

  3. 化学工学会   バイオ部会幹事、環境生物分野専門分科会代表、東海支部幹事

  4. American Society for Microbiology

  5. 農芸化学会

  6. American Society for Biochemistry and Molecular Biology

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Committee Memberships 9

  1. 日本化学会   バイオテクノロジー部会幹事  

    2024.3   

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    Committee type:Academic society

  2. 生物工学会   理事  

    2011.1   

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    Committee type:Academic society

  3. 化学工学会   バイオ部会幹事  

    2010.4   

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    Committee type:Academic society

  4. みえバイオリファイナリー研究会   オブザーバー  

    2014.7 - 2015.3   

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    Committee type:Municipal

  5. 水の先進理工学第183委員会   委員  

    2013.4 - 2015.3   

  6. 化学工学会   代議員  

    2013.3 - 2015.2   

  7. 生物工学会   英文誌J.Biosci. Bioeng. 編集委員  

    2008.4 - 2013.5   

  8. 化学工学会   バイオ部会幹事  

    2005.4   

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    Committee type:Academic society

  9. 化学工学会   東海支部幹事  

    2005.4 - 2012.3   

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Awards 8

  1. 生物工学功績賞

    2023.6   日本生物工学会   細菌の接着機構と界面微生物工学プロセスの創出に関する研究

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    Award type:International academic award (Japan or overseas) 

  2. 研究賞(内藤雅喜記念賞)

    2023.3   化学工学会   微生物接着と革新的固定化微生物プロセスの構築

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    Award type:International academic award (Japan or overseas)  Country:Japan

  3. Excellent Poster Award

    2019.9   APCChE 2019  

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    Award type:Award from international society, conference, symposium, etc.  Country:Japan

  4. 生物工学論文賞

    2013.9   日本生物工学会  

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    Country:Japan

  5. Best Presentation Award, APBioChech'09

    2009.11   APBioChech  

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    Country:Japan

  6. 名古屋工業大学褒賞 優秀賞受賞

    2007.11   名古屋工業大学  

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    Country:Japan

    「微生物の粘着に着目したユニークな研究実績,対外評価と外部資金獲得」

  7. 竹田国際貢献賞

    2004.9   竹田理科工業株式会社  

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    Country:Japan

  8. 2001年度化学工学会優秀論文賞

    2001   化学工学会  

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    Country:Japan

    K. Hori et al., Isolation, characterization and application to off-gas treatment of toluene degrading bacteria; J. Chem. Eng. Japan, 34, (2001) 1120-1126

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Papers 142

  1. Adhesion preference of the sticky bacterium Acinetobacter sp. Tol 5 Reviewed International coauthorship International journal

    S. Yoshimoto, S. Ishii, A. Kawashiri, T. Matsushita, D. Linke, S. Gottig, VAJ. Kempf, M. Takai, and K. Hori

    Frontiers in Bioengineering and Biotechnolgoy   Vol. 12   page: 1342418   2024.2

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Gram-negative bacterium Acinetobacter sp. Tol 5 exhibits high adhesiveness to various surfaces of general materials, from hydrophobic plastics to hydrophilic glass and metals, via AtaA, an Acinetobacter trimeric autotransporter adhesin Although the adhesion of Tol 5 is nonspecific, Tol 5 cells may have prefer materials for adhesion. Here, we examined the adhesion of Tol 5 and other bacteria expressing different TAAs to various materials, including antiadhesive surfaces. The results highlighted the stickiness of Tol 5 through the action of AtaA, which enabled Tol 5 cells to adhere even to antiadhesive materials, including polytetrafluoroethylene with a low surface free energy, a hydrophilic polymer brush with steric hindrance, and mica with an ultrasmooth surface. Single-cell force spectroscopy as an atomic force microscopy technique revealed the strong cell adhesion force of Tol 5 to these antiadhesive materials. Nevertheless, Tol 5 cells showed a weak adhesion force toward a zwitterionic 2-methacryloyloxyethyl-phosphorylcholine (MPC) polymer-coated surface. Dynamic flow chamber experiments revealed that Tol 5 cells, once attached to the MPC polymer-coated surface, were exfoliated by weak shear stress. The underlying adhesive mechanism was presumed to involve exchangeable, weakly bound water molecules. Our results will contribute to the understanding and control of cell adhesion of Tol 5 for immobilized bioprocess applications and other TAA-expressing pathogenic bacteria of medical importance.

    DOI: 10.3389/fbioe.2024.1342418

  2. Electrochemical monnitoring of metabolic activity of methsne/methanol conversing Methylococcus capsulatus (Bath) cells based on extrancellular electron transfer Reviewed International coauthorship International journal

    K. Sugimoto, K. Hori, M. Ishikawa, H. Ito, T. Kamachi, K. Tanaka, Y-Y. Chen, and S. Nakanishi

    Electrochemistry   Vol. 92 ( 2 ) page: 022007   2024.2

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    Language:English   Publishing type:Research paper (scientific journal)  

    Bioconversion of methane to methanol by methanotrophs under mild conditions is a promising approach for efficiently utilizing methane. Here, we present an electrochemical technique based on open-circuit potential (OCP) measurements to monitor the metabolic activity of Methylococcus capsulatus (Bath), a representative methanotrophic model. This technique
    is based on the extracellular electron transfer (EET) mechanism, in which intracellular electrons in living cells are exchanged across the cell membrane with an extracellular electrode. Without using artificial electron mediators in our study, we observed that OCP shifted to negative when methane metabolism was activated. By manipulating the culture conditions with the absence or presence of copper supplement to regulate the expression of outer membrane cytochromes (OMCs), the cells with a high OMC expression level, known to serve as conduits for EET, responded with increased sensitivity to stimulation with excess NADH compared to the cells with a low OMC expression level. We, therefore, used the instinctive EET capacity of M. capsulatus (Bath) for real-time OCP measurement to monitor the bioconversion of methane to methanol. Our measurements showed that the OCP levels change with
    intracellular redox variations and reflect methanol production rates. Our findings may facilitate the development of a methanotrophic bioprocess that allows more effective and efficient control of intracellular redox status using OCP monitoring based on EET.

    DOI: https://doi.org/10.5796/electrochemistry.23-68120

  3. Long-term continuous degradation of carbon nanotubes by a bacteria-driven Fenton reaction Reviewed International journal

    S.Takahashi, and K. Hori

    Frontiers in Microbiology   Vol. 14   page: 1298323   2023.11

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Very few bacteria are known that can degrade carbon nanotubes (CNTs), and the only known degradation mechanism is a Fenton reaction driven by Labrys sp. WJW with siderophores, which only occurs under iron-deficient conditions. No useful information is available on the degradation rates or long-term stability and continuity of the degradation reaction although several months or more are needed for CNT degradation. In this study, we investigated long-term continuous degradation of oxidized (carboxylated) single-walled CNTs (O-SWCNTs) using bacteria of the genus Shewanella. These bacteria are widely present in the environment and can drive the Fenton reaction by alternating anaerobic-aerobic growth conditions under more general environmental conditions. We first examined the effect of O-SWCNTs on the growth of S. oneidensis MR-1, and it was revealed that O-SWCNTs promote growth up to 30 μg/mL but inhibit growth at 40 μg/mL and above. Then, S. oneidensis MR-1 was subjected to incubation cycles consisting of 21-h anaerobic and 3-h aerobic periods in the presence of 30 μg/mL O-SWCNTs and 10 mM Fe(III) citrate. We determined key factors that help prolong the bacteria-driven Fenton reaction and finally achieved long-term continuous degradation of O-SWCNTs over 90 d. By maintaining a near neutral pH and replenishing Fe(III) citrate at 60 d, a degraded fraction of 56.3% was reached. S. oneidensis MR-1 produces Fe(II) from Fe(III) citrate, a final electron acceptor for anaerobic respiration during the anaerobic period. Then, ·OH is generated through the Fenton reaction by Fe(II) and H2O2 produced by MR-1 during the aerobic period. ·OH was responsible for O-SWCNT degradation, which was inhibited by scavengers of H2O2 and ·OH. Raman spectroscopy and X-ray photoelectron spectroscopy showed that the graphitic structure in O-SWCNTs was oxidized, and electron microscopy showed that long CNT fibers initially aggregated and became short and isolated during degradation. Since Shewanella spp. and iron are ubiquitous in the environment, this study suggests that a Fenton reaction driven by this genus is applicable to the degradation of CNTs under a wide range of conditions and will help researchers develop novel methods for waste treatment and environmental bioremediation against CNTs.

    DOI: https://doi.org/10.3389/fmicb.2023.1298323

  4. Contribution of the Fenton reaction to the degradation of carbon nanotubes by enzymes Reviewed International journal

    S. Takahashi, F. Taguchi and K. Hori

    Frontiers in Environmental Science   Vol. 11   2023.6

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3389/fenvs.2023.1184257

  5. Simple method for the creation of a bacteria-sized unilamellar liposome with different proteins localized to the respective sides of the membrane Reviewed International journal

    K. Noba, S. Yoshimoto, Y. Tanaka, T. Yokoyama, T. Matsuura ,and K. Hori

    ACS Synthetic Biology   Vol. 12 ( 5 ) page: 1437 - 1446   2023.5

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    DOI: 10.1021/acssynbio.2c00564

  6. Growth phase-dependent production of the adhesive nanofiber protein AtaA in Acinetobacter sp. Tol 5 Reviewed International journal

    Ishikawa Masahito, Nakatani Hajime, Hori Katsutoshi

    JOURNAL OF BIOSCIENCE AND BIOENGINEERING   Vol. 135 ( 3 ) page: 224 - 231   2023.3

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Bioscience and Bioengineering  

    AtaA, the sticky, long, and peritrichate nanofiber protein from Acinetobacter sp. Tol 5, mediates autoagglutination and is highly adhesive to various material surfaces, resulting in a biofilm. Although the production of the adhesive nanofiber protein is likely to require a large amount of energy and material sources, the relationship between AtaA fiber production and cell growth remains unknown. Here, we report the growth phase-dependent AtaA fiber production in Tol 5. We examined the ataA gene expression in different growth phases using a reporter gene assay with an originally developed reporter plasmid and using reverse transcription-quantitative polymerase chain reaction. Bacterial cells with surface-displayed AtaA at different growth phases were immunostained and analyzed using fluorescence flow cytometry and confocal laser scanning microscopy. The results indicate that Tol 5 modulated the amount of surface-displayed AtaA at the transcriptional level. AtaA production was low in the early growth phase but remarkably increased in the late growth phase, covering the whole bacterial cell with AtaA fibers in the stationary phase. Tol 5 displayed AtaA fibers poorly in the early growth phase and showed less autoagglutination and adhesiveness than those in the stationary phase. Although Tol 5 grew as fast as its ataA-deficient mutant in the early growth phase, the optical density of Tol 5 culture was slightly lower than that of the ataA-deficient mutant in the late growth phase. Based on these experimental results, we propose the growth-phase-dependent production of AtaA fiber for efficient and fast cell growth.

    DOI: 10.1016/j.jbiosc.2022.12.012

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  7. A novel inverse membrane bioreactor for efficient bioconversion from methane gas to liquid methanol using a microbial gas-phase reaction Reviewed International journal

    Chen Yan-Yu, Ishikawa Masahito, Hori Katsutoshi

    BIOTECHNOLOGY FOR BIOFUELS AND BIOPRODUCTS   Vol. 16 ( 1 ) page: 16   2023.2

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Biotechnology for Biofuels and Bioproducts  

    ackground: Methane (CH4), as one of the major energy sources, easily escapes from the supply chain into the atmosphere, because it exists in a gaseous state under ambient conditions. Compared to carbon dioxide (CO2), CH4 is 25 times more potent at trapping radiation; thus, the emission of CH4 to the atmosphere causes severe global warming and climate change. To mitigate CH4 emissions and utilize them effectively, the direct biological conversion of CH4 into liquid fuels, such as methanol (CH3OH), using methanotrophs is a promising strategy. However, supplying biocatalysts in an aqueous medium with CH4 involves high energy consumption due to vigorous agitation and/or bubbling, which is a serious concern in methanotrophic processes, because the aqueous phase causes a very large barrier to the delivery of slightly soluble gases. Results: An inverse membrane bioreactor (IMBR), which combines the advantages of gas-phase bioreactors and membrane bioreactors, was designed and constructed for the bioconversion of CH4 into CH3OH in this study. In contrast to the conventional membrane bioreactor with bacterial cells that are immersed in an aqueous phase, the filtered cells were placed to face a gas phase in the IMBR to supply CH4 directly from the gas phase to bacterial cells. Methylococcus capsulatus (Bath), a representative methanotroph, was used to demonstrate the bioconversion of CH4 to CH3OH in the IMBR. Cyclopropanol was supplied from the aqueous phase as a selective inhibitor of methanol dehydrogenase, preventing further CH3OH oxidation. Sodium formate was added as an electron donor to generate NADH, which is necessary for CH3OH production. After optimizing the inlet concentration of CH4, the mass of cells, the cyclopropanol concentration, and the gas flow rate, continuous CH3OH production can be achieved over 72 h with productivity at 0.88 mmol L−1 h−1 in the IMBR, achieving a longer operation period and higher productivity than those using other types of membrane bioreactors reported in the literature. Conclusions: The IMBR can facilitate the development of gas-to-liquid (GTL) technologies via microbial processes, allowing highly efficient mass transfer of substrates from the gas phase to microbial cells in the gas phase and having the supplement of soluble chemicals convenient.

    DOI: 10.1186/s13068-023-02267-6

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  8. Identification of the adhesive domain of AtaA from Acinetobacter sp. Tol 5 and its application in immobilizing Escherichia coli Reviewed International coauthorship International journal

    Yoshimoto Shogo, Aoki Sota, Ohara Yuki, Ishikawa Masahito, Suzuki Atsuo, Linke Dirk, Lupas Andrei N., Hori Katsutoshi

    FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY   Vol. 10   page: 1095057   2023.1

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Frontiers in Bioengineering and Biotechnology  

    Cell immobilization is an important technique for efficiently utilizing whole-cell biocatalysts. We previously invented a method for bacterial cell immobilization using AtaA, a trimeric autotransporter adhesin from the highly sticky bacterium Acinetobacter sp. Tol 5. However, except for Acinetobacter species, only one bacterium has been successfully immobilized using AtaA. This is probably because the heterologous expression of large AtaA (1 MDa), that is a homotrimer of polypeptide chains composed of 3,630 amino acids, is difficult. In this study, we identified the adhesive domain of AtaA and constructed a miniaturized AtaA (mini-AtaA) to improve the heterologous expression of ataA. In-frame deletion mutants were used to perform functional mapping, revealing that the N-terminal head domain is essential for the adhesive feature of AtaA. The mini-AtaA, which contains a homotrimer of polypeptide chains from 775 amino acids and lacks the unnecessary part for its adhesion, was properly expressed in E. coli, and a larger amount of molecules was displayed on the cell surface than that of full-length AtaA (FL-AtaA). The immobilization ratio of E. coli cells expressing mini-AtaA on a polyurethane foam support was significantly higher compared to the cells with or without FL-AtaA expression, respectively. The expression of mini-AtaA in E. coli had little effect on the cell growth and the activity of another enzyme reflecting the production level, and the immobilized E. coli cells could be used for repetitive enzymatic reactions as a whole-cell catalyst.

    DOI: 10.3389/fbioe.2022.1095057

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  9. Perturbation by Antimicrobial Bacteria of the Epidermal Bacterial Flora of Rainbow Trout in Flow-Through Aquaculture Reviewed International journal

    Nakatani Hajime, Yamada Naoki, Hashimoto Naoki, Okazaki Fumiyoshi, Arakawa Tomoko, Tamaru Yutaka, Hori Katsutoshi

    BIOLOGY-BASEL   Vol. 11 ( 8 ) page: 1249   2022.8

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    The bacterial flora of the epidermal mucus of fish is closely associated with the host’s health and susceptibility to pathogenic infections. In this study, we analyzed the epidermal mucus bacteria of rainbow trout (Oncorhynchus mykiss) reared in flow-through aquaculture under environmental perturbations. Over ~2 years, the bacteria present in the skin mucus and water were analyzed based on the 16S rDNA sequences. The composition of the mucus bacterial community showed significant monthly fluctuations, with frequent changes in the dominant bacterial species. Analysis of the beta- and alpha-diversity of the mucus bacterial flora showed the fluctuations of the composition of the flora were caused by the genera Pseudomonas, Yersinia, and Flavobacterium, and some species of Pseudomonas and Yersinia in the mucus were identified as antimicrobial bacteria. Examination of the antimicrobial bacteria in the lab aquarium showed that the natural presence of antimicrobial bacteria in the mucus and water, or the purposeful addition of them to the rearing water, caused a transition in the mucus bacteria community composition. These results demonstrate that specific antimicrobial bacteria in the water or in epidermal mucus comprise one of the causes of changes in fish epidermal mucus microflora.

    DOI: 10.3390/biology11081249

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  10. Fabrication of a highly protective 3D-printed mask and evaluation of its viral filtration efficiency using a human head mannequin Reviewed International journal

    Ohara Yuki, Kanie Junichi, Hori Katsutoshi

    HARDWAREX   Vol. 11   page: e00314   2022.4

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    Facemasks are one of the most effective and low-cost prophylactics for COVID-19. In the spring 2020, when a severe shortage of facemasks occurred worldwide, various types of 3D-printed masks were designed and proposed. However, the protective effects conferred by most of these masks were not experimentally evaluated. Here, we provide a new simple design of 3D-printed mask and evaluate its protective effect in a viral filtration test using a human head mannequin. The developed mask can be constructed with a low-cost 3D printer, with an approximate production cost of US $4. This mask has three parts: the main part, wearing parts, and a piece of non-woven fabric filter. The volume of the filter, which needs to be changed daily, was reduced to approximately 1/10 of that of commercially available surgical masks used in this study. The developed mask is fabricated from polylactic acid, a biodegradable plastic, and its surface contour contacting the face may be adjusted after softening the material with hot water at 60–80 °C. The viral filtration efficiency of the developed mask was found to be over 80%. This performance is better than that of commercially available facemasks, such as surgical masks and cloth masks, and equal to those of KN95 and KF94.

    DOI: 10.1016/j.ohx.2022.e00314

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  11. Single-cell adhesion force mapping of a highly sticky bacterium in liquid Reviewed International journal

    Satoshi Ishii, Shogo Yoshimoto, Katsutoshi Hori

    JOURNAL OF COLLOID AND INTERFACE SCIENCE   Vol. 606 ( Pt 1 ) page: 628 - 634   2022.1

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Journal of Colloid and Interface Science  

    The sticky bacterium Acinetobacter sp. Tol 5 adheres to various material surfaces via its cell surface nanofiber protein, AtaA. This adhesiveness has only been evaluated based on the amount of cells adhering to a surface. In this study, the adhesion force mapping of a single Tol 5 cell in liquid using the quantitative imaging mode of atomic force microscopy (AFM) revealed that the adhesion of Tol 5 was near 2 nN, which was 1–2 orders of magnitude higher than that of other adhesive bacteria. The adhesion force of a cell became stronger with the increase in AtaA molecules present on the cell surface. Many fibers of peritrichate AtaA molecules simultaneously interact with a surface, strongly attaching the cell to the surface. The adhesion force of a Tol 5 cell was drastically reduced in the presence of 1% casamino acids but not in deionized water (DW), although both liquids decrease the adhesiveness of Tol 5 cells, suggesting that DW and casamino acids inhibit the cell approaching step and the subsequent direct interaction step of AtaA with surfaces, respectively. Heterologous production of AtaA provided non-adhesive Acinetobacter baylyi ADP1 cells with a strong adhesion force to AFM tip surfaces of silicon and gold.

    DOI: 10.1016/j.jcis.2021.08.039

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  12. Recent advances in research on biointerfaces: From cell surfaces to artificial interfaces Invited Reviewed International journal

    K. Hori, S. Yoshimoto, T. Yoshino, T. Zako, G. Hirao, S. Fujita, C. Nakamura, A. Yamagishi, and N. Kamiya

    Journal of Bioscience and Bioengineering   Vol. 133 ( 3 ) page: 195 - 207   2022.1

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    Biointerfaces are regions where biomolecules, cells, and organic materials are exposed to environmental media or come in contact with other biomaterials, cells, and inorganic/organic materials. In this review article, six research topics on biointerfaces are described to show examples of state-of-art research approaches. First, biointerface design of nanoparticles for molecular detection is described. Functionalized gold nanoparticles can be used for sensitive detection of various target molecules, including chemical compounds and biomolecules, such as DNA, proteins, cells, and viruses. Second, the interaction between bacterial cell surfaces and material surfaces, including the introduction of advances in analytical methods and theoretical calculations, are explained as well as their applications to bioprocesses. Third, bioconjugation technologies for localizing functional proteins at biointerfaces are introduced, in particular, by focusing the potential of enzymes as a catalytic tool for designing different types of bioconjugates that function at biointerfaces. Forth topics is focusing on lipideprotein interaction in cell membranes as natural biointerfaces. Examples of membrane lipid engineering are introduced, and it is mentioned how their compositional profiles affect membrane protein functions. Fifth topic is the physical method for molecular delivery across the biointerface being developed currently, such as highly efficient nanoinjection, electroporation, and nanoneedle devices, in which the key is how to perforate the cell membrane. Final topic is the chemical design of lipid- or polymer-based RNA delivery carriers and their behavior on the cell interface, which are currently attracting attention as RNA vaccine technologies targeting COVID-19. Finally, future directions of biointerface studies are presented.

  13. Complete Genome Sequence of the Highly Adhesive Bacterium Acinetobacter sp. Strain Tol 5 Reviewed International journal

    Masahito Ishikawa, Katsutoshi Hori

    Microbiology Resource Announcements   Vol. 10 ( 35 ) page: e00567-21   2021.9

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    Acinetobacter sp. strain Tol 5 is a nonpathogenic Gram-negative bacterium with biotechnological and environmental applications. Here, we report the complete genome sequence of Acinetobacter sp. strain Tol 5, which has a genome size of 4,799,506 bp and a G1C content of 38.1%.

    DOI: 10.1128/MRA.00567-21

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  14. Development of a Biocontained Toluene-Degrading Bacterium for Environmental Protection Reviewed International journal

    Masahito Ishikawa, Takaaki Kojima, Katsutoshi Hori

    MICROBIOLOGY SPECTRUM   Vol. 9 ( 1 ) page: 1 - 11   2021.9

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:American Society for Microbiology  

    Biocontainment is a safeguard strategy for preventing uncontrolled proliferation of genetically engineered microorganisms (GEMs) in the environment. Biocontained GEMs are designed to survive only in the presence of a specific molecule. The design of a pollutant-degrading and pollutant-dependent GEM prevents its proliferation after cleaning the environment. In this study, we present a biocontained toluene-degrading bacterium based on Acinetobacter sp. Tol 5. The bamA gene, which encodes an essential outer membrane protein, was deleted from the chromosome of Tol 5 but complemented with a plasmid carrying a bamA gene regulated by the Pu promoter and the regulatory protein XylR. The resultant strain (PuBamA) degraded toluene, similarly to the wild-type Tol 5. Although the cell growth of the PuBamA strain was remarkably inhibited after toluene depletion, escape mutants emerged at a frequency of 1 per 5.3 × 10−7 cells. Analyses of escape mutants revealed that insertion sequences (ISs) carrying promoters were inserted between the Pu promoter and the bamA gene on the complemented plasmid. MinION deep sequencing of the plasmids extracted from the escape mutants enabled the identification of three types of ISs involved in the emergence of escape mutants, suggesting a strategy for reducing it. IMPORTANCE GEMs are beneficial for various applications, including environmental protection. However, the risks of GEM release into the environment have been debated for a long time. If a pollutant is employed as a specific molecule for a biocontainment system, GEMs capable of degrading pollutants are available for environmental protection. Nevertheless, to our knowledge, biocontained degraders for real pollutants have not been reported in academic journals so far. This is possibly due to the difficulty in the expression of enzymes for degrading pollutants in a tractable bacterium such as Escherichia coli. On the other hand, bacteria with enzymes for degrading pollutants are often intractable as a host of GEMs due to the shortage of tools for genetic manipulation. This study reports the feasibility of a biocontainment strategy for a toluene degrader. Our results provide useful insights into the construction of a GEM biocontainment system for environmental protection.

    DOI: 10.1128/Spectrum.00259-21

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  15. Metabolic alteration of Methylococcus capsulatus str. Bath during a microbial gas-phase reaction Reviewed International journal

    Yan-Yu Chen, Yuki Soma, Masahito Ishikawa, Masatomo Takahashi, Yoshihiro Izumi, Takeshi Bamba, Katsutoshi Hori

    BIORESOURCE TECHNOLOGY   Vol. 330   page: 125002   2021.6

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    Authorship:Last author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier  

    This study demonstrates the metabolic alteration of Methylococcus capsulatus (Bath), a representative bacterium among methanotrophs, in microbial gas-phase reactions. For comparative metabolome analysis, a bioreactor was designed to be capable of supplying gaseous substrates and liquid nutrients continuously. Methane degradation by M. capsulatus (Bath) was more efficient in a gas-phase reaction operated in the bioreactor than in an aqueous phase reaction operated in a batch reactor. Metabolome analysis revealed remarkable alterations in the metabolism of cells in the gas-phase reaction; in particular, pyruvate, 2-ketoglutarate, some amino acids, xanthine, and hypoxanthine were accumulated, whereas 2,6-diaminopimelate was decreased. Based on the results of metabolome analysis, cells in the gas-phase reaction seemed to alter their metabolism to reduce the excess ATP and NADH generated upon increased availability of methane and oxygen. Our findings will facilitate the development of efficient processes for methane-based bioproduction with low energy consumption.

    DOI: 10.1016/j.biortech.2021.125002

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  16. Switching between methanol accumulation and cell growth by expression control of methanol dehydrogenase in Methylosinus trichosporium OB3b mutant Invited Reviewed International journal

    Hidehiro Ito, Kosei Yoshimori, Masahito Ishikawa, Katsutoshi Hori, Toshiaki Kamachi

    Frontiers in Microbiology   Vol. 12   page: 639266   2021.3

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    Methanotrophs have been used to convert methane to methanol at ambient temperature and pressure. In order to accumulate methanol using methanotrophs, methanol dehydrogenase (MDH) must be downregulated as it consumes methanol. Here, we describe a methanol production system wherein MDH expression is controlled by using methanotroph mutants. We used the MxaF knockout mutant of Methylosinus trichosporium OB3b. It could only grow with MDH (XoxF) which has a cerium ion in its active site and is only expressed by bacteria in media containing cerium ions. In the presence of 0 μM copper ion and 25 μM cerium ion, the mutant grew normally. Under conditions conducive to methanol production (10 μM copper ion and 0 μM cerium ion), cell growth was inhibited and methanol accumulated (2.6 μmol·mg−1 dry cell weight·h−1). The conversion efficiency of the accumulated methanol to the total amount of methane added to the reaction system was ~0.3%. The aforementioned conditions were repeatedly alternated by modulating the metal ion composition of the bacterial growth medium.

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  17. Gas-phase bioproduction of a high-value-added monoterpenoid (E)-geranic acid by metabolically engineered Acinetobacter sp. Tol 5 Reviewed International journal

    Atsushi Usami, Masahito Ishikawa, Katsutoshi Hori

    Green Chemistry   Vol. 22 ( 4 ) page: 1258 - 1268   2020.2

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    Gas-phase bioproduction, in which immobilized biocatalysts are employed and chemical reactions are performed in a gas phase, has attracted researchers' attention as a green process. However, there is difficulty in the employment of whole cell catalysts for gas-phase bioproduction due to the lack of a suitable cell immobilization method. Acinetobacter sp. Tol 5 is a unique bacterium, which is remarkably sticky and can be easily immobilized onto various material surfaces through the adhesive bacterionanofiber protein AtaA. In this study, we demonstrate the gas-phase bioproduction of (E)-geranic acid (GA), a high-value-added monoterpenoid, from geraniol using immobilized Tol 5 transformant cells, into which a gene involved in a (E)-GA synthetic pathway was introduced. Time course analysis of the liquid-phase bioproduction of (E)-GA revealed the inherent metabolism of Tol 5 involved in the degradation of (E)-GA. By disrupting the fadD4-ortholog gene, which encodes a key enzyme of the (E)-GA degradation, we successfully generated a (E)-GA-accumulating strain, Tol 5 ΔfadD4 (pGeoA). The immobilized cells of this mutant strain on a polyurethane support enabled the production of (E)-GA with a passive supply of gaseous geraniol in a batch gas-phase reaction. A major fraction of the (E)-GA, which was produced, was adsorbed onto the polyurethane support but easily extracted into ethanol, a safe solvent without environmental impact. This is the first example of gas-phase bioproduction of a complex and high-value-added compound. Tol 5 is a highly promising platform for gas-phase bioproduction.

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  18. Process Description of an Unconventional Biofilm Formation by Bacterial Cells Autoagglutinating through Sticky, Long, and Peritrichate Nanofibers Reviewed International journal

    Furuichi Yoshihide, Yoshimoto Shogo, Inaba Tomohiro, Nomura Nobuhiko, Hori Katsutoshi

    Environmental Science & Technology   Vol. 54 ( 4 ) page: 2520 - 2529   2020.2

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    In this study, we elucidated the formation process of an unconventional biofilm formed by a bacterium autoagglutinating through sticky, long, and peritrichate nanofibers. Understanding the mechanisms of biofilm formation is essential to control microbial behavior and improve environmental biotechnologies. Acinetobacter sp. Tol 5 autoagglutinate through the interaction of the long, peritrichate nanofiber protein AtaA, a trimeric autotransporter adhesin. Using AtaA, without cell growth or extracellular polymeric substances production, Tol 5 cells quickly form an unconventional biofilm. The process forming this unconventional biofilm started with cell-cell interactions, proceeded to cell clumping, and led to the formation of large cell aggregates. The cell-cell interaction was described by Derjaguin-Landau-Verwey-Overbeek (DLVO) theory based on a new concept, which considers two independent interactions between two cell bodies and between two AtaA fiber tips forming a discontinuous surface. If cell bodies cannot collide owing to an energy barrier at low ionic strengths but approach within the interactive distance of AtaA fibers, cells can agglutinate through their contact. Cell clumping proceeds following the cluster-cluster aggregation model, and an unconventional biofilm containing void spaces and a fractal nature develops. Understanding its formation process would extend the utilization of various types of biofilms, enhancing environmental biotechnologies.

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  19. Evaluation of methane degradation performance in microbial gas-phase reactions using effectively immobilized methanotrophs Reviewed International journal

    Yan-Yu Chen, Masahito Ishikawa, Risa Suzuki, Hidehiro Ito, Toshiaki Kamachi, Katsutoshi Hori

    Biochemical Engineering Journal   Vol. 154   page: 107441   2020.2

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    The removal of atmospheric methane by methanotrophs is a potential strategy for mitigating global warming. However, due to the low mass transfer of methane to methanotrophic cells, the conventional bioprocesses require active gas-supply with high energy consumption. This study aimed to evaluate methane degradation performance in microbial gas-phase reactions using effectively immobilized Methylococcus capsulatus (Bath) cells. Cell immobilization was achieved by freeze-thaw treatment and a simple filtration process. The methane degradation rate was much faster in the gas-phase reaction than in the aqueous-phase reaction with stirring. The efficiency of methane degradation was almost the same between the gas-phase reaction using gel-entrapped cells and the static aqueous reaction using a cell suspension. The results of this study will facilitate the development of a high-efficiency and low-energy bioprocess for methane removal.

    DOI: 10.1016/j.bej.2019.107441

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  20. Bottom-up Creation of an Artificial Cell Covered with the Adhesive Bacterionanofiber Protein AtaA Reviewed International journal

    Kosaku Noba, Masahito Ishikawa, Atsuko Uyeda, Takayoshi Watanabe, Takahiro Hohsaka, Shogo Yoshimoto, Tomoaki Matsuura, Katsutoshi Hori

    JOURNAL OF THE AMERICAN CHEMICAL SOCIETY   Vol. 141 ( 48 ) page: 19058 - 19066   2019.12

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    The bacterial cell surface structure has important roles for various cellular functions. However, research on reconstituting bacterial cell surface structures is limited. This study aimed to bottom-up create a cell-sized liposome covered with AtaA, the adhesive bacterionanofiber protein localized on the cell surface of Acinetobacter sp. Tol 5, without the use of the protein secretion and assembly machineries. Liposomes containing a benzylguanine derivative-modified phospholipid were decorated with a truncated AtaA protein fused to a SNAP-tag expressed in a soluble fraction in Escherichia coli. The obtained liposome showed a similar surface structure and function to that of native Tol 5 cells and adhered to both hydrophobic and hydrophilic solid surfaces. Furthermore, this artificial cell was able to drive an enzymatic reaction in the adhesive state. The developed artificial cellular system will allow for analysis of not only AtaA, but also other cell surface proteins under a cell-mimicking environment. In addition, AtaA-decorated artificial cells may inspire the development of biotechnological applications that require immobilization of cells onto a variety of solid surfaces, in particular, in environments where the use of genetically modified organisms is prohibited.

    DOI: 10.1021/jacs.9b09340

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  21. Control of AtaA-mediated bacterial immobilization by casein hydrolysates. Reviewed International journal

    Y. Ohara, S. Yoshimoto, K. Hori

    Journal of bioscience and bioengineering   Vol. 128 ( 5 ) page: 544 - 550   2019.11

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    Acinetobacter sp. Tol 5 exhibits an autoagglutinating nature and high adhesiveness to various abiotic surfaces through its bacterionanofiber protein AtaA. We have developed new bacterial immobilization methods utilizing the high adhesiveness of AtaA. We previously reported that salt is essential for the adhesiveness of AtaA. In the current study, we unexpectedly found that Tol 5 cells were not immobilized onto polyurethane foam support during growth in LB medium although AtaA was properly expressed and displayed onto the cell surface. The adhesion of Tol 5 resting cells was not affected by sugars but drastically inhibited by yeast extract and casein hydrolysates such as tryptone and casamino acids technical grade (CA-T). Some amino acids, which are major components of CA-T, partially inhibited the adhesion of Tol 5 cells. Experimental results suggested that oligopeptides might effectively inhibit the cell adhesion. Immobilized cells onto the support through AtaA were detached in CA-T solution. Also, the detached cells could be re-immobilized onto the support without impairing of their adhesiveness by replacing CA-T solution to a basal salt medium. Microscopic observation revealed that breaking of AtaA-mediated cell–cell interaction is important for the detachment of Tol 5 cells from the support. CA-T also inhibited AtaA-mediated autoagglutination and dispersed cell clumps through AtaA. This is the first report on adhesion inhibitors against AtaA and suggests that casein hydrolysates like CA-T would be a powerful tool for controlling AtaA-mediated bacterial immobilization.

    DOI: 10.1016/j.jbiosc.2019.04.019

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  22. 細菌の接着機構と界面微生物工学プロセスの創出に関する研究

    堀 克敏

    生物工学会誌   Vol. 第102巻 ( 第2号 ) page: 54 - 60   2024

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  23. 微生物飼料を活用した次世代陸上養殖の展開

    田丸 浩、堀 克敏

    月刊バイオインダストリー   Vol. 40 ( 1 ) page: 51 - 57   2023.1

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  24. 小型化接着タンパク質による大腸菌の固定化と微生物反応への応用; バイオサイエンスとインダストリー

    吉本 将悟、堀 克敏

    バイオサイエンスとインダストリー   Vol. 40   page: 51 - 57   2023

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  25. 細菌接着タンパク質と微生物固定化への利用 Invited

    堀 克敏

    バイオインダストリー   Vol. 39 ( 3 ) page: 11 - 18   2022.3

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  26. ウイルス除去効果の高い樹脂製立体マスクの開発 Invited

    堀 克敏

    繊維機械学会誌 月刊せんい   Vol. 74 ( 11 ) page: 593 - 598   2021.11

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  27. 界面微生物工学 微生物固定化・細胞表層工学から膜タンパク質利用技術まで Invited

    堀 克敏

    化学と生物   Vol. 59 ( 8 ) page: 393 - 400   2021.8

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  28. Establishing a percutaneous infection model using zebrafish and a salmon pathogen Invited Reviewed International journal

    Hajime Nakatani and Katsutoshi Hori

    Biology   Vol. 10 ( 2 ) page: 166   2021.2

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    The epidermis and mucus layer of fish act as barriers that protect them against waterborne pathogens, and provide niches for symbiotic microorganisms that benefit the host’s health. However, our understanding of the relationship between fish skin bacterial flora and fish pathogen infection is limited. In order to elucidate this relationship, an experimental model for
    infection through fish skin is necessary. Such a model must also pose a low biohazard risk in a laboratory setting. We established a percutaneous infection model using zebrafish (Danio rerio), a typical fish experimental model, and Yersinia ruckeri, a salmon pathogen. Our experimental data indicate that Y. ruckeri colonizes niches on the skin surface generated by transient changes in the skin microflora caused by stress, dominates the skin bacterial flora, occupies the surface of the fish skin, invades the fish body through injury, and finally, causes fatal enteric redmouth disease. This percutaneous infection model can be used to study the interaction between fish skin bacterial flora and fish pathogens in water, or the relationship between pathogens and the host’s skin immune system.

  29. 微生物接着タンパク質AtaAの無限の可能性 Invited

    堀 克敏

    バイオインダストリー   Vol. 38 ( 2 ) page: 8 - 17   2021.2

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  30. The extracellular juncture domains in the Intimin passenger adopt a constitutively extended conformation inducing restrains to its sphere of action Reviewed International coauthorship International journal

    J. Weikum, A. Kulakova, G. Tesei, S. Yoshimoto, L. V. Jægerum, M. Schütz, K. Hori, M. Skepö, P. Harris, J. C. Leo, and J. P. Morth

    Scientific Reports   Vol. 10 ( 1 ) page: 21249   2020.12

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    Enterohemorrhagic and enteropathogenic Escherichia coli are among the most important food-borne pathogens, posing a global health threat. The virulence factor intimin is essential for the attachment of pathogenic E. coli to the intestinal host cell. Intimin consists of four extracellular bacterial immunoglobulin-like (Big) domains, D00–D2, extending into the fifth lectin subdomain (D3) that binds to the Tir-receptor on the host cell. Here, we present the crystal structures of the elusive D00–D0 domains at 1.5 Å and D0–D1 at 1.8 Å resolution, which confirms that the passenger of intimin has five distinct domains. We describe that D00–D0 exhibits a higher degree of rigidity and D00 likely functions as a juncture domain at the outer membrane-extracellular medium interface. We conclude that D00 is a unique Big domain with a specific topology likely found in a broad range of other inverse autotransporters. The accumulated data allows us to model the complete passenger of intimin and propose functionality to the Big domains, D00–D0–D1, extending directly from the membrane.

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  31. 人工細胞と固体表面をつなぐ接着ナノファイバー~新規生体触媒の開発を目指して~ Invited

    石川 聖人,堀 克敏

    バイオサイエンスとインダストリー   Vol. 78 ( 5 ) page: 408 - 409   2020.9

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  32. Native display of a huge homotrimeric protein fiber on the cell surface after precise domain deletion Reviewed International coauthorship International journal

    S. Aoki, S. Yoshimoto, M. Ishikawa, D. Linke, A. Lupas, and K. Hori

    J. Biosci. Bioeng   Vol. 129 ( 4 ) page: 412 - 417   2020.4

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    AtaA, a trimeric autotransporter adhesin from Acinetobacter sp. Tol 5, exhibits nonspecific, high adhesiveness to abiotic surfaces. For identification of the functional domains of AtaA, precise design of domain-deletion mutants is necessary so as not to cause undesirable structural distortion. Here, we designed and constructed three types of AtaA mutants from which the same domain, FGG1, was deleted. The first mutant was designed to preserve the periodicity of hydrophobic residues in the coiled-coil segments sandwiching the deleted region. After the deletion, the protein was properly displayed on the cell surface and had the same adhesive function as the wild type. Transmission electron microscopy (TEM) imaging and circular dichroism (CD) spectroscopy showed that its isolated passenger domain had the same fiber structure as in the AtaA wild type. In contrast, a mutant designed to disturb the coiled-coil periodicity at the deletion site failed to reach the cell surface. Although secretion occurred for the mutant designed with a flexible connector between the coiled coils, the cells exhibited a decrease in adhesiveness. Furthermore, TEM imaging of the mutant fibers showed bending at the fiber tip and changes in their CD spectrum indicated a decrease in secondary structure content. Thus, we succeeded to natively display the huge homotrimeric fiber structure of AtaA on the cell surface after precise deletion of a domain, maintaining the proper folding state and adhesive function by preserving its coiled-coil periodicity. This strategy enables us to construct various domain-deletion mutants of AtaA without structural distortion for complete functional mapping.

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  33. Extracellular electron transfer mediated by a cytocompatible redox polymer to study the crosstalk among the mammalian circadian clock, cellular metabolism, and cellular redox state Reviewed International journal

    Masahito Ishikawa, Kazuki Kawai, Masahiro Kaneko, Kenya Tanaka, Shuji Nakanishi, Katsutoshi Hori

    RSC ADVANCES   Vol. 10 ( 3 ) page: 1648 - 1657   2020.1

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    The circadian clock is an endogenous biological timekeeping system that controls various physiological and cellular processes with a 24 h rhythm. The crosstalk among the circadian clock, cellular metabolism, and cellular redox state has attracted much attention. To elucidate this crosstalk, chemical compounds have been used to perturb cellular metabolism and the redox state. However, an electron mediator that facilitates extracellular electron transfer (EET) has not been used to study the mammalian circadian clock due to potential cytotoxic effects of the mediator. Here, we report evidence that a cytocompatible redox polymer pMFc (2-methacryloyloxyethyl phosphorylcholine-co-vinyl ferrocene) can be used as the mediator to study the mammalian circadian clock. EET mediated by oxidized pMFc (ox-pMFc) extracted intracellular electrons from human U2OS cells, resulting in a longer circadian period. Analyses of the metabolome and intracellular redox species imply that ox-pMFc receives an electron from glutathione, thereby inducing pentose phosphate pathway activation. These results suggest novel crosstalk among the circadian clock, metabolism, and redox state. We anticipate that EET mediated by a redox cytocompatible polymer will provide new insights into the mammalian circadian clock system, which may lead to the development of new treatments for circadian clock disorders.

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  34. 接着性人工細胞触媒のボトムアップ創製 Invited

    石川 聖人,堀 克敏

    生物工学   Vol. 98   page: 651 - 654   2020

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  35. 接着タンパク質・接着微生物を利用した効率的バイオプロセスの構築 Invited

    石川 聖人,堀 克敏

    酵素工学ニュース   Vol. 84   page: 13 - 17   2020

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  36. 接着性バクテリオナノファイバーによる微生物のプロセスの革新 Invited Reviewed

    堀 克敏

    環境バイオテクノロジー学会誌   Vol. 19   page: 21 - 28   2019.3

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  37. On-fiber display of a functional peptide at sites distant from the cell surface using a long bacterionanofiber of a trimeric autotransporter adhesin Reviewed International journal

    Hajime Nakatani, Junichi Kanie, Katsutoshi Hori

    BIOTECHNOLOGY AND BIOENGINEERING   Vol. 116 ( 2 ) page: 239 - 249   2019.2

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    In the cell surface display system, the distance of a surface-displayed molecule from the cell surface should influence its functionality due to the interference by other surface structures. For the purpose of developing this distance-variable surface display system, we utilized a long fibrous adhesin, Acinetobacter trimeric autotransporter adhesin (AtaA) of the strain Tol 5. We constructed His-tagged full-length and shorter AtaA fibers designed by N-terminal deletion and expressed them in the ΔataA mutant. Immunoelectron microscopy clearly showed that they formed fibers on the cell surface and the His-tag was displayed on the fiber tip located at fixed distances from the cell surface. N-terminal deletion of AtaA shortened the distance between the His-tag and the cell surface, as designed. Time-course analyses of the cell-to-Ni-Sepharose beads binding revealed that cells producing the longer fibers bound more rapidly to the beads. The His-tagged AtaA derivatives were also displayed on Escherichia coli cells, and a similar tendency was shown; the His-tag on the longer fiber was more functional than that on the shorter one. Thus, we developed an on-fiber display system of a functional peptide using a long trimeric autotransporter adhesin (TAA) fiber, which can vary the distance between the displayed molecule and the cell surface.

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  38. The Acinetobacter trimeric autotransporter adhesin Ata controls key virulence traits of Acinetobacter baumannii Reviewed International coauthorship International journal

    Marko Weidensdorfer, Masahito Ishikawa, Katsutoshi Hori, Dirk Linke, Bardya Djahanschiri, Ruben Iruegas, Ingo Ebersberger, Sara Riedel-Christ, Giulia Enders, Laura Leukert, Peter Kraiczy, Florian Rothweiler, Jindrich Cinatl, Juergen Berger, Katharina Hipp, Volkhard A. J. Kempf, Stephan Goettig

    VIRULENCE   Vol. 10 ( 1 ) page: 68 - 81   2019.1

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    Acinetobacter baumannii is a Gram-negative pathogen that causes a multitude of nosocomial infections. The Acinetobacter trimeric autotransporter adhesin (Ata) belongs to the superfamily of trimeric autotransporter adhesins which are important virulence factors in many Gram-negative species. Phylogenetic profiling revealed that ata is present in 78% of all sequenced A. baumannii isolates but only in 2% of the closely related species A. calcoaceticus and A. pittii. Employing a markerless ata deletion mutant of A. baumannii ATCC 19606 we show that adhesion to and invasion into human endothelial and epithelial cells depend on Ata. Infection of primary human umbilical cord vein endothelial cells (HUVECs) with A. baumannii led to the secretion of interleukin (IL)-6 and IL-8 in a time- and Ata-dependent manner. Furthermore, infection of HUVECs by WT A. baumannii was associated with higher rates of apoptosis via activation of caspases-3 and caspase-7, but not necrosis, in comparison to ∆ata. Ata deletion mutants were furthermore attenuated in their ability to kill larvae of Galleria mellonella and to survive in larvae when injected at sublethal doses. This indicates that Ata is an important multifunctional virulence factor in A. baumannii that mediates adhesion and invasion, induces apoptosis and contributes to pathogenicity in vivo.

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  39. Efficient Counterselection for Methylococcus capsulatus (Bath) by Using a Mutated pheS Gene Reviewed International journal

    Masahito Ishikawa, Sho Yokoe, Souichiro Kato, Katsutoshi Hori

    APPLIED AND ENVIRONMENTAL MICROBIOLOGY   Vol. 84 ( 23 ) page: e01875-18   2018.12

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    Methylococcus capsulatus (Bath) is a representative gammaproteobacterial methanotroph that has been studied extensively in diverse research fields. The sacB gene, which encodes levansucrase, causing cell death in the presence of sucrose, is widely used as a counterselectable marker for disruption of a target gene in Gram-negative bacteria. However, sacB is not applicable to all Gram-negative bacteria, and its efficiency for the counterselection of M. capsulatus (Bath) is low. Here, we report the construction of an alternative counterselectable marker, pheS*, by introduction of two point mutations (A306G and T252A) into the pheS gene from M. capsulatus (Bath), which encodes the α-subunit of phenylalanyl-tRNA synthetase. The transformant harboring pheS* on an expression plasmid showed sensitivity to 10 mM p-chloro-phenylalanine, whereas the transformant harboring an empty plasmid showed no sensitivity, indicating the availability of pheS* as a counterselectable marker in M. capsulatus (Bath). To validate the utility of the pheS* marker in counterselection, we attempted to obtain an unmarked mutant of xoxF, a gene encoding the major subunit of Xox methanol dehydrogenase, which we failed to obtain by counterselection using the sacB marker. PCR, immunodetection using an anti-XoxF antiserum, and a cell growth assay in the absence of calcium demonstrated successful disruption of the xoxF gene in M. capsulatus (Bath). The difference in counterselection efficiencies of the markers indicated that pheS* is more suitable than sacB for counterselection in M. capsulatus (Bath). This study provides a new genetic tool enabling efficient counterselection in M. capsulatus (Bath).

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  40. Extracellular Electron Transfer via Outer Membrane Cytochromes in a Methanotrophic Bacterium Methylococcus capsulatus (Bath) Reviewed International journal

    Kenya Tanaka, Sho Vokoe, Kensuke Igarashi, Motoko Takashino, Masahito Ishikawa, Katsutoshi Hori, Shuji Nakanishi, Souichiro Kato

    FRONTIERS IN MICROBIOLOGY   Vol. 9 ( NOV ) page: 2905   2018.11

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    Electron exchange reactions between microbial cells and solid materials, referred to as extracellular electron transfer (EET), have attracted attention in the fields of microbial physiology, microbial ecology, and biotechnology. Studies of model species of iron-reducing, or equivalently, current-generating bacteria such as Geobacter spp. and Shewanella spp. have revealed that redox-active proteins, especially outer membrane c-type cytochromes (OMCs), play a pivotal role in the EET process. Recent (meta)genomic analyses have revealed that diverse microorganisms that have not been demonstrated to have EET ability also harbor OMC-like proteins, indicating that EET via OMCs could be more widely preserved in microorganisms than originally thought. A methanotrophic bacterium Methylococcus capsulatus (Bath) was reported to harbor multiple OMC genes whose expression is elevated by Cu starvation. However, the physiological role of these genes is unknown. Therefore, in this study, we explored whether M. capsulatus (Bath) displays EET abilities via OMCs. In electrochemical analysis, M. capsulatus (Bath) generated anodic current only when electron donors such as formate were available, and could reduce insoluble iron oxides in the presence of electron donor compounds. Furthermore, the current-generating and iron-reducing activities of M. capsulatus (Bath) cells that were cultured in a Cu-deficient medium, which promotes high levels of OMC expression, were higher than those cultured in a Cu-supplemented medium. Anodic current production by the Cu-deficient cells was significantly suppressed by disruption of MCA0421, a highly expressed OMC gene, and by treatment with carbon monoxide (CO) gas (an inhibitor of c-type cytochromes). Our results provide evidence of EET in M. capsulatus (Bath) and demonstrate the pivotal role of OMCs in this process. This study raises the possibility that EET to solid compounds is a novel survival strategy of methanotrophic bacteria.

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  41. Heterologous expression of geraniol dehydrogenase for identifying the metabolic pathways involved in the biotransformation of citral by Acinetobacter sp Tol 5 Reviewed International journal

    Usami Atsushi, Masahito Ishikawa, Katsutoshi Hori

    BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY   Vol. 82 ( 11 ) page: 2012 - 2020   2018.11

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    The biotransformation of citral, an industrially important monoterpenoid, has been extensively studied using many microbial biocatalysts. However, the metabolic pathways involved in its biotransformation are still unclear, because citral is a mixture of the trans-isomer geranial and the cis-isomer neral. Here, we applied the heterologous expression of geoA, a gene encoding geraniol dehydrogenase that specifically converts geraniol to geranial and nerol to neral, to identify the metabolic pathways involved in the biotransformation of citral. Acinetobacter sp. Tol 5 was employed in order to demonstrate the utility of this methodology. Tol 5 transformed citral to (1R,3R,4R)-1-methyl-4-(1-methylethenyl)-1,3-cyclohexanediol and geranic acid. Biotransformation of citral precursors (geraniol and nerol) by Tol 5 transformant cells expressing geoA revealed that these compounds were transformed specifically from geranial. Our methodology is expected to facilitate a better understanding of the metabolic pathways involved in the biotransformation of substrates that are unstable and include geometric isomers.

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  42. Cell behavior of the highly sticky bacterium Acinetobacter sp Tol 5 during adhesion in laminar flows Reviewed International journal

    Yoshihide Furuichi, Keita Iwasaki, Katsutoshi Hori

    SCIENTIFIC REPORTS   Vol. 8 ( 1 ) page: 8285   2018.5

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    It is important to characterize how medically, industrially, or environmentally important bacteria adhere to surfaces in liquid flows in order to control their cell adhesion and subsequent biofilm formation. Acinetobacter sp. Tol 5 is a remarkably sticky bacterium that autoagglutinates through the adhesive nanofiber protein AtaA, which is applicable to cell immobilization in bioprocesses. In this study, the adhesion and behavior of Tol 5 cells in laminar flows were investigated using flow cell systems. Tol 5 cells autoagglutinated through AtaA and formed cell clumps during flowing. The cell clumps rather than single cells went downward due to gravity and adhered to the bottom surface. Under appropriate shear stress, a twin vortex was caused by a separated flow generated at the rear of the pre-immobilized cell clumps and carried the small cell clumps to this location, resulting in their stacking there. The rearward immobilized cell clumps developed into a large, stable aggregate with a streamlined shape, independent of cell growth. Cell clumps hardly ever developed under weak shear stress that could not generate a twin vortex and were broken up under excessively strong shear stress. These cell behaviors including the importance of clumping are interesting features in the bacterial adhesion processes.

    DOI: 10.1038/s41598-018-26699-5

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  43. Immobilization of Enterobacter aerogenes by a Trimeric Autotransporter Adhesin, AtaA, and Its Application to Biohydrogen Production Invited Reviewed International journal

    Hajime Nakatani, Ding Nan, Yuki Ohara, Katsutoshi Hori

    CATALYSTS   Vol. 8 ( 4 ) page: 159   2018.4

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    Biological hydrogen production by microbial cells has been extensively researched as an energy-efficient and environmentally-friendly process. In this study, we propose a fast, easy method for immobilizing Enterobacter aerogenes by expressing ataA, which encodes the adhesive protein of Acinetobacter sp. Tol 5. AtaA protein on the E. aerogenes cells carrying the ataA gene was demonstrated by immunoblotting and flow cytometry. The AtaA-producing cells exhibited stronger adherence and auto-agglutination characteristics than wild-type cells, and were successfully immobilized (at approximately 2.5 mg/cm3) on polyurethane foam. Hydrogen production from the cell-immobilized polyurethane foams was monitored in repetitive batch reactions and flow reactor studies. The total hydrogen production in triple-repetitive batch reactions reached 0.6 mol/mol glucose, and the hydrogen production rate in the flow reactor was 42 mL·h−1·L−1. The AtaA production achieved simple and immediate immobilization of E. aerogenes on the foam, enabling repetitive and continuous hydrogen production. This report newly demonstrates the production of AtaA on the cell surfaces of bacterial genera other than Acinetobacter, and can simplify and accelerate the immobilization of whole-cell catalysts.

    DOI: 10.3390/catal8040159

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  44. Real-time monitoring of intracellular redox changes in Methylococcus capsulatus (Bath) for efficient bioconversion of methane to methanol Reviewed International journal

    Masahito Ishikawa, Yuya Tanaka, Risa Suzuki, Kota Kimura, Kenya Tanaka, Kazuhide Kamiya, Hidehiro Ito, Souichiro Kato, Toshiaki Kamachi, Katsutoshi Hori, Shuji Nakanishi

    BIORESOURCE TECHNOLOGY   Vol. 241   page: 1157 - 1161   2017.10

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    This study aimed to develop a novel method for real-time monitoring of the intracellular redox states in a methanotroph Methylococcus capsulatus, using Peredox as a genetically encoded fluorescent sensor of the NADH:NAD+ ratio. As expected, the fluorescence derived from the Peredox-expressing M. capsulatus transformant increased by supplementation of electron donor compounds (methane and formate), while it decreased by specifically inhibiting the methanol oxidation reaction. Electrochemical measurements confirmed that the Peredox fluorescence reliably represents the intracellular redox changes. This study is the first to construct a reliable redox-monitoring method for methanotrophs, which will facilitate to develop more efficient methane-to-methanol bioconversion processes.

    DOI: 10.1016/j.biortech.2017.05.107

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  45. Reversible bacterial immobilization based on the salt-dependent adhesion of the bacterionanofiber protein AtaA Reviewed International journal

    Shogo Yoshimoto, Yuki Ohara, Hajime Nakatani, Katsutoshi Hori

    MICROBIAL CELL FACTORIES   Vol. 16 ( 1 ) page: 123   2017.7

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    Background: Immobilization of microbial cells is an important strategy for the efficient use of whole-cell catalysts because it simplifies product separation, enables the cell concentration to be increased, stabilizes enzymatic activity, and permits repeated or continuous biocatalyst use. However, conventional immobilization methods have practical limitations, such as limited mass transfer in the inner part of a gel, gel fragility, cell leakage from the support matrix, and adverse effects on cell viability and catalytic activity. We previously showed a new method for bacterial cell immobilization using AtaA, a member of the trimeric autotransporter adhesin family found in Acinetobacter sp. Tol 5. This approach is expected to solve the drawbacks of conventional immobilization methods. However, similar to all other immobilization methods, the use of support materials increases the cost of bioprocesses and subsequent waste materials. Results: We found that the stickiness of the AtaA molecule isolated from Tol 5 cells is drastically diminished at ionic strengths lower than 10 mM and that it cannot adhere in deionized water, which also inhibits cell adhesion mediated by AtaA. Cells immobilized on well plates and polyurethane foam in a salt solution were detached in deionized water by rinsing and shaking, respectively. The detached cells regained their adhesiveness in a salt solution and could rapidly be re-immobilized. The cells expressing the ataA gene maintained their adhesiveness throughout four repeated immobilization and detachment cycles and could be repeatedly immobilized to polyurethane foam by a 10-min shake in a flask. We also demonstrated that both bacterial cells and a support used in a reaction could be reused for a different type of reaction after detachment of the initially immobilized cells from the support and a subsequent immobilization step. Conclusions: We invented a unique reversible immobilization method based on the salt-dependent adhesion of the AtaA molecule that allows us to reuse bacterial cells and supports by a simple manipulation involving a deionized water wash. This mitigates problems caused by the use of support materials and greatly helps to enhance the efficiency and productivity of microbial production processes.

    DOI: 10.1186/s12934-017-0740-7

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  46. バイオコントロールによる高濃度油脂含有排水のハイパフォーマンス処理 Invited Reviewed

    堀 克敏

    環境バイオテクノロジー学会誌   Vol. 17   page: 3-8   2017.3

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  47. Cell surface protein engineering for high-performance whole-cell catalysts Invited Reviewed International journal

    Nakatani Hajime, Hori Katsutoshi

    FRONTIERS OF CHEMICAL SCIENCE AND ENGINEERING   Vol. 11 ( 1 ) page: 46 - 57   2017.3

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    Cell surface protein engineering facilitated by accumulation of information on genome and protein structure involves heterologous production and modification of cell surface proteins using genetic engineering, and is important for the development of high-performance whole-cell catalysts. In this field, cell surface display is a major technology by exposing target proteins, such as enzymes, on the cell surface using a carrier protein. The target proteins are fused to the carrier proteins that transport and tether them to the cell surface, as well as to a secretion signal. This paper reviews cell surface display systems for prokaryotic and eukaryotic cells from the perspective of carrier proteins, which determine the number of displayed molecules, and the localization, size, and direction (N- or C-terminal anchoring) of the passengers. We also discuss advanced methods for displaying multiple enzymes and a new method for the immobilization of whole-cell catalysts using adhesive surface proteins.

    DOI: 10.1007/s11705-017-1609-3

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  48. バイオフィルム・微生物付着の工学利用

    堀 克敏

    膜   Vol. 42   page: 54-59   2017

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  49. 細胞表層タンパク質の合成生物学 Invited

    石川聖人、堀 克敏

    生物工学会誌   Vol. 94   page: 701-703   2016.11

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  50. 細菌の持つ接着ナノファイバータンパク質AtaA

    堀 克敏

    バイオサイエンスとインダストリー   Vol. 74   page: 380-381   2016.11

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  51. Secretion of the intimin passenger domain is driven by protein folding Reviewed

    J. C. Leo, P. Oberhettinger, S. Yoshimoto, D. B. R. K. G. Udatha, J. P. Morth, M. Schütz, K. Hori, and D. Linke

    J. Biol. Chem   Vol. 291   page: 20096   2016.7

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    DOI: 10.1074/jbc.M116.731497

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  52. An Acinetobacter trimeric autotranspoter adhesin reaped from cells exhibits its nonspecific stickiness via a highly stable 3D strucuture Reviewed

    S. Yoshimoto, H. Nakatani, K.Iwasaki and K.Hori

    Sci Rep     2016.6

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    DOI: 10.1038/srep28020

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  53. Discovery of a novel periplasmic protein that forms acomplex with a trimeric autotransporter adhesin and Peptidoglycan Reviewed

    M. Ishikawa, S. Yoshimoto, A. Hayashi, J. Kanie and K.Hori

    Mol Microbiol     2016.5

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    DOI: 10.1111/mmi.13398

  54. 細菌接着タンパク質AtaA分子の特性と応用 Invited

    堀 克敏

    バイオサイエンスとインダストリー   Vol. 74   page: 417-419   2016.5

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  55. Structural basis for toughness and flexibility in the C-terminal Passenger domain of an Acinetobactor trimeric autotranspoter Adhesin Reviewed

    K. Koiwai, M.D.Hartmann, D. Linke, A.N. Lupas and K.Hori

    J. Biol. Chem.   Vol. 291   page: 3705-3724   2016.2

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    DOI: 10.1074/jbc.M115.701698

  56. Effectiveness of direct immobilization of bacterial cells onto material surfaces using the bacterionanofiber protein AtaA. Reviewed

    Hori K, Ohara Y, Ishikawa M, Nakatani H.

    Applied Microbiology and Biotechnology     2015.4

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    The bacterionanofiber protein AtaA, a member of the trimeric autotransporter adhesin family found in Acinetobacter sp. Tol 5, is responsible for the nonspecific, high adhesiveness and autoagglutination of this strain. Previously, we introduced the ataA gene into the nonadhesive Acinetobacter strain ST-550, which conferred high adhesiveness to this strain, immobilized its cells, and improved indigo productivity due to enhanced tolerance to the toxic substrate. In this study, we again demonstrated the effectiveness of this new microbial immobilization method using AtaA in a number of conditions. AtaA enabled the effective immobilization of growing, resting, and lyophilized cells of a type strain of Acinetobacter, ADP1, which is also intrinsically nonadhesive, onto the surface of several kinds of support ranging from artificial to natural materials and from hydrophobic polyurethane to hydrophilic glass. Immobilization with AtaA enabled exclusive cell growth in the support space and only a few cells existed in the bulk medium. Immobilization of resting cells drastically increased cell concentration, depending on the support material; dry cells of approximately 110 g/L could be immobilized onto glass wool. Finally, we demonstrated that ADP1 cells immobilized on polyurethane foam can undergo at least 10 repetitive reactions without inactivation during a 5-h period. Even after drying and storing for 3 days, the immobilized cells showed enzymatic activity and an ester hydrolysis reaction was repeated by simply transferring the support with the cells into a fresh reaction buffer.

    DOI: 10.1007/s00253-015-6554-9

    PubMed

  57. Definition of the transcription factor TdIF1 consensus-binding sequence through genomewide mapping of its binding sites Reviewed

    K. Koiwai,T. Kubota, N. Watanabe, K. Hori, O. Koiwai and H. Masai

    Genes to Cells   Vol. 20 ( 3 ) page: 242-254   2015.1

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    TdIF1 was originally identified as a protein that directly binds to terminal deoxynucleotidyltransferase, TdT. Through in vitro selection assays (SELEX), we recently showed that TdIF1 recognizes both AT-tract and a specific DNA sequence motif, 5'-TGCATG-3', and can up-regulate the expression of RAB20 through the latter motif. However, whether TdIF1 binds to these sequences in the cells has not been clear and its other target genes remain to be identified. Here, we determined in vivo TdIF1-binding sequences (TdIF1-invivoBMs) on the human chromosomes through ChIP-seq analyses. The result showed a 160-base pair cassette containing 'AT-tract~palindrome (inverted repeat)~AT-tract' as a likely target sequence of TdIF1. Interestingly, the core sequence of the palindrome in the TdIF1-invivoBMs shares significant similarity to the above 5'-TGCATG-3' motif determined by SELEX in vitro. Furthermore, spacer sequences between AT-tract and the palindrome contain many potential transcription factor binding sites. In luciferase assays, TdIF1 can up-regulate transcription activity of the promoters containing the TdIF1-invivoBM, and this effect is mainly through the palindrome. Clusters of this motif were found in the potential target genes. Gene ontology analysis and RT-qPCR showed the enrichment of some candidate targets of TdIF1 among the genes involved in the regulation of ossification. Potential modes of transcription activation by TdIF1 are discussed.

    DOI: 10.1111/gtc.12216

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  58. バイオフィルムを利用した界面微生物工学

    堀克敏

    化学療法の領域   Vol. 31 ( 11 ) page: 88-96   2015

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  59. Application of the adhesive bacterionanofiber AtaA to a novel microbial immobilization method for the production of indigo as a model chemical Reviewed

    M. Ishikawa, K. Sugimori, K. Hori

    Biotechnol. Bioeng.   Vol. 111   page: 16-24   2014.1

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    DOI: 10.1002/bit.25012

  60. TdiF1 recognizes a specific DNA sequence through its helix-turn-helix and AT-hook motifs to regulate gene transcription Reviewed

    T. Kubota, O. Koiwai, K. Hori, N. Watanabe, K. Koiwai

    PLoS ONE   Vol. 8   page: e66710   2013

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  61. Extracellular electron transfer of a highly adhesive and metabolically versatile bacterium Reviewed

    H. Liu, M. Ishikawa, S. Matsuda, Y. Kimoto, K. Hori, K. Hashimoto, S. Nakanishi

    ChemPhysChem   Vol. 14   page: 2407-2412   2013

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  62. A new simple method for introducing an unmarked mutation into a large gene of non-competent Gram-negative bacteria by FLP/FRT recombination Reviewed

    M. Ishikawa, K. Hori

    BMC Microbiology   Vol. 13   page: 86   2013

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  63. 炭素繊維が微生物を集めて水を浄化するメカニズム

    松本慎也,堀克敏

    繊維機械学会誌   ( 66 ) page: 29-33   2013

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  64. AtaA, a new member of the trimeric autotransporter adhesins from Acinetobacter sp. Tol 5 mediating high adhesiveness to various abiotic surfaces Reviewed

    M. Ishikawa, H. Nakatani, K. Hori

    PLoS ONE   Vol. 7   page: e48830   2012.11

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  65. Carbon fiber as an Excellent Support Material for Wastewater Treatment Biofilms Reviewed

    Shinya Matsumoto, Akihito Ohtaki, Katsutoshi Hori

    Environmental Science & Technology   Vol. 46   page: 10175-10181   2012.9

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  66. Evaluation of adhesiveness of Acinetobacter sp.Tol5 to abiotic surfaces Reviewed

    M. Ishikawa, K. Shigemori, A. Suzuki, and K. Hori

    J. Biosci. Bioeng.   Vol. 113   page: 719-725   2012.6

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  67. バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築 Invited

    堀 克敏

    化学工学   Vol. 76 ( 4 ) page: 212-214   2012.4

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  68. 界面微生物工学によるグリーン・イノベーションを目指して Invited

    堀 克敏

    化学工業   Vol. 63 ( 1 ) page: 70-74   2012.1

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  69. 微生物付着・バイオフィルムの制御と応用から界面微生物工学 Reviewed

    堀 克敏

    オレオサイエンス   Vol. 11   page: 19-24   2012

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  70. バクテリオナノファイバーの細胞接着機能と応用 Invited

    堀 克敏

    高分子   Vol. 60 ( 10 ) page: 747-748   2011.6

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  71. Production of peritrichate bacterionanofibers and their proteinaceous components by Acinetobacter sp. Tol 5 cell affected by growth substrates Reviewed

      Vol. 111   page: 31-36   2011

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  72. Simultaneous syntheses of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa IFO3924 at various temperatures and from various fatty acids Reviewed

    K. Hori, R. Ichinohe, H. Unno, and S. Marsudi

    Biochem. Eng. J   Vol. 53   page: 196-202   2011

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  73. バクテリオナノファイバーによる細菌の細胞接着 Invited

    堀 克敏

      Vol. 25   page: 1-7   2011

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  74. Effects of microbial degradation of biofoulants on microfiltration membrane performance in a membrane bioreactor Reviewed

    D. Okamura, Y. Mori, T. Hashimoto, and K. Hori

    Environ. Sci. Technol   Vol. 44   page: 8644-8648   2010

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  75. Bacterial adhesion: From mechanism to control Invited Reviewed

    K. Hori, and S. Matsumoto

    Biochem. Eng. J   Vol. 48   page: 424-434   2010

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  76. 微生物の付着機構 Reviewed

    堀 克敏

    日本接着学会誌   Vol. 46   page: 70-75   2010

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  77. 細菌ナノファイバーによる微生物の表面付着 Invited Reviewed

    堀 克敏、石川聖人

    環境バイオテクノロジー学会誌   Vol. 10   page: 3-7   2010

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  78. Production of triacylglycerol and poly(3-hydroxybutyrate-co-3-hydroxyvalerate) by the toluene-degrading bacterium Rhodococcus aetherivorans IAR1 Reviewed

    K. Hori, M. Abe, and H. Unno

    J. Biosci. Bioeng   Vol. 108   page: 319-324   2009.10

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  79. Drastic change in cell surface hydrophobicity of a new bacterial strain, Pseudomonas sp. TIS1-127, induced by growth temperature and its effects on the toluene-conversion rate Reviewed

    K. Hori, N. Hiramatsu, M. Nannbu, K. Kanie, M. Okochi, H. Honda, and H. Watanabe

    J. Biosci. Bioeng   ( 107 ) page: 250-255   2009.9

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  80. Rhodococcus aetherivorans IAR1, a new bacterial strain synthesizing poly(3-hydroxybutyrate-co-3-hydroxyvalerate) from toluene Reviewed

    K. Hori, A. Kobayashi, H. Ikeda, H. Unno

    J. Biosci. Bioeng   Vol. 107   page: 145-150   2009.8

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  81. 細菌ナノファイバーの構造と細胞付着機構 Invited

    堀 克敏

    バイオサイエンスとインダストリー   Vol. 67   page: 409-412   2009.8

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  82. 粘着性微生物の構造・機能解析の現状 Invited

    堀 克敏

    機械の研究,   Vol. 61   page: 783-788   2009.8

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  83. 改良ネガティブ染色法によるAcinetobacter属細菌Tol5株細胞上の3種粘着性ナノファイバーの識別 Reviewed

    樋口愛介、堀田康明、山本宏治、堀 克敏

    医学生物学電子顕微鏡技術学会誌   Vol. 23   page: 9-14   2009.7

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  84. Identification of biofoulant of membrane bioreactors in soluble microbial products Reviewed

    D. Okamura, Y. Mori, T. Hashimoto, and K. Hori

    Water Res   Vol. 43   page: 4356-4362   2009.6

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  85. Symbiotic effects of a lipase-secreting bacterium, Burkholderia arboris SL1B1, and a glycerol-assimilating yeast, Candida cylindracea SL1B2, on triacylglycerol degradation Reviewed

    H. Matsuoka, A. Miura, K. Hori

    J. Biosci. Bioeng   Vol. 107   page: 401-408   2009.4

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  86. Rapid conversion of toluene by an Acinetobacter sp. Tol 5 mutant showing monolayer adsorption to oil-water interface Reviewed

    H. Watanabe, Y. Tanji, H. Unno, K. Hori

    J. Biosci. Bioeng   Vol. 106   page: 226-230   2008

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  87. Formation of filamentous appendages by Acinetobacter sp. Tol 5 to adhere to solid surfaces Reviewed

    S. Ishii, S. Miyata, Y. Hotta, K. Yamamoto, H. Unno, and K. Hori

    J. Biosci. Bioeng   Vol. 105   page: 20-25   2008

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  88. Palm oil utilization for simultaneous production of polyhydroxyalkanaotes and rhamnolipids by Pseudomonas aeruginosa Reviewed

    S. Marsudi, H. Unno, K. Hori

    Appl. Microbiol. Biotechnol   Vol. 78   page: 955-961   2008

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  89. Monolayer adsorption of a bald mutant of the highly adhesive and hydrophobic bacterium, Acinetobacter sp. Tol 5, to a hydrocarbon surface Reviewed

    K. Hori, H. Watanabe, S. Ishii, Y. Tanji, and H. Unno

    Appl. Environ. Microbiol   Vol. 74   page: 2511-2517   2008

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  90. 微生物の付着に働く粘着性細菌ナノファイバー Invited Reviewed

    堀 克敏

    ケミカルエンジニヤリング   Vol. 53   page: 830-835   2008

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  91. Optimization of distinction between viable and dead cells by fluorescent staining method and its application to bacterial consortia Reviewed

    K. Miyanaga, S. Takano, Y. Morono, K. Hori, H. Unno and Y. Tanji

    Biochem. Eng. J   Vol. 37   page: 56-61   2007

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  92. Effect of cell appendages on the adhesion property of a highly adhesive bacterium, Acinetobacter sp. Tol 5 Reviewed

    S. Ishii, H. Unno, S. Miyata, K. Hori

    Biosci. Biotech. Biochem   Vol. 70   page: 2635-2640   2006

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  93. Purification and characterization of fungal poly(3-hydroxybutyrate) depolymerase from Paecilomyces lilacinus F4-5 and enzymatic degradation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) film Reviewed

    B-I. Sang, W-K. Lee, K. Hori, and H. Unno

    World J. Microbiol. Biotechnol   Vol. 22   page: 51-57   2006

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  94. Correlation of TCE cometabolism with growth characteristics on aromatic substrates in toluene-degrading bacteria Reviewed

    Y. Morono, H. Unno, K. Hori

    Biochem. Eng. J.   Vol. 31   page: 173-179   2006

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  95. 細菌に強付着性をもたらす新規細胞表層構造 Invited Reviewed

    堀 克敏、石井俊一、海野 肇

    医学生物学電子顕微鏡技術学会誌   Vol. 20   page: 3-5   2006

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  96. 環境汚染物質の微生物分解と無害化

    堀 克敏

    分離技術   Vol. 36   page: 79-83   2006

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  97. メタン発酵共生微生物群が形成する凝集体のSEM観察 Reviewed

    石井俊一、堀 克敏、堀田康明、渡辺一哉

    医学生物学電子顕微鏡技術学会誌   Vol. 20   page: 6-8   2006

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  98. Coaggregation facilitates interspecies hydrogen transfer between Pelotomaculum thermopropionicum and Methanothermobacter thermautotrophicus Reviewed

    S. Ishii, T. Kosaka, K. Hori, Y. Hotta, and K. Watanabe

    Appl. Environ. Microbiol   Vol. 71   page: 7838-7845   2005

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  99. Effect of spacer length on beta-lactoglobulin hydrolysis by trypsin covalently immobilized on a cellulosic support Reviewed

    S. Yamamoto, A. Imamura, I. Susanti, K. Hori, Y. Tanji and H. Unno

    Food and Bioproducts Processing   Vol. 83   page: 61-67   2005

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  100. Kinetic analyses of trichloroethylene cometabolism by toluene-degrading bacteria harboring a tod homologous gene Reviewed

    K. Hori, J. Mii, Y. Morono, Y. Tanji, and H. Unno

    Biochem. Eng. J   Vol. 26   page: 59-64   2005

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  101. 強付着性細菌が有するアンカー様表層構造物

    堀 克敏

    バイオサイエンスとインダストリー   Vol. 63   page: 295-296   2005

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    Language:Japanese  

  102. Toward rational control of Escherichia coli O157:H7 by a phage cocktail Reviewed

    Y. Tanji, T. Shimada, M. Yoichi, K. Miyanaga, K. Hori, and H. Unno

    Appl. Microbiol. Biotechnol   Vol. 64   page: 270-274   2004

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  103. Addition of aromatic substrates restores trichloroethylene degradation activity in Pseudomonas putida F1 Reviewed

    Y. Morono, H. Unno, Y. Tanji and K. Hori

    Appl. Environ. Microbiol   Vol. 70   page: 2830-2835   2004

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  104. Two morphological types of cell appendages on a strongly adhesive bacterium, Acinetobacter sp. strain Tol 5 Reviewed

    S. Ishii, J. Koki, H. Unno and K. Hori

    Appl. Environ. Microbiol   Vol. 70   page: 5026-5029   2004

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  105. A mathematical description for the fungal degradation process of biodegradable plastics Reviewed

    B-I. Sang, K. Hori, and H. Unno

    Mathematics and Computers in Simulation   Vol. 65   page: 147-155   2004

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  106. Application of glutaraldehyde for the staining of esterase-active cells with carboxyfluorescein diacetate Reviewed

    Y. Morono, S. Takano, K. Miyanaga, Y. Tanji, H. Unno, and K. Hori

    Biotech. Lett   Vol. 26   page: 379-383   2004

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  107. Bacillus amyloliquefaciens phage endolysin can enhance permeability of Pseudomonas aeruginosa outer membrane and induce cell lysis Reviewed

    Y. Orito, M. Morita, K. Hori, and H. Unno, Y. Tanji

    Appl. Microbiol. Biotechnol   Vol. 65   page: 105-109   2004

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  108. Formation, sedimentation and germination properties of Anabaena akinetes Reviewed

    K. Hori, J. Okamoto, Y. Tanji and H. Unno

    Biochem. Eng. J   Vol. 14   page: 67-73   2003

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  109. Augmentation of self-purification capacity of sewer pipe by immobilizing microbes on the pipe surface Reviewed

    I. W. Marjaka, K. Miyanaga, K. Hori, Y. Tanji and H. Unno

    Biochem. Eng. J   Vol. 15   page: 69-75   2003

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  110. PHBVフィルムの土壌中でのオンサイト分解におけるカビの寄与

    海野 肇、堀 克敏

    高分子加工   Vol. 52   page: 277-282   2003

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  111. 微生物機能を利用した環境保全技術―水環境を中心として―;

    海野 肇、堀 克敏

    化学装置 別冊     page: 7-11   2003

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  112. Effect of dispersing oil phase on the biodegradability of a solid alkane dissolved in non-biodegradable oil Reviewed

    K. Hori, Y. Matsuzaki, Y. Tanji and H. Unno

    Appl. Microbiol. Biotechnol   Vol. 59   page: 574-579   2002

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  113. Construction of self-disruptive Bacillus megaterium in response to substrate exhaustion for polyhydroxybutyrate production Reviewed

    K. Hori, M. Kaneko, Y. Tanji, X.-H. Xing and H. Unno

    Appl. Microbiol. Biotechnol   Vol. 59   page: 211-216   2002

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  114. 微生物機能を利用した環境保全技術―汚染防止・浄化システムの現状と研究動向―

    海野 肇、堀 克敏

    化学装置   Vol. 44   page: 160-166   2002

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  115. 微生物膜に対するバイオサイド(5-chloro-2-methyl-4-isothiazolin-3-one; CMI) の抗菌活性 Reviewed

    丹治保典、中野 肇、堀 克敏、宮永一彦、海野 肇

    材料と環境   Vol. 51   page: 561-565   2002

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  116. Fate of coliphage in waste water treatment process and detection of phages carrying the shiga toxin type 2 Reviewed

    Y. Tanji, K. Mizoguchi, T. Akitsu, M. Morita, K. Hori and H. Unno

    Water Science and Technology   Vol. 46   page: 285-289   2002

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  117. Fate of coliphage in a wastewater treatment process Reviewed

    Y. Tanji, K. Mizoguchi, M. Yoichi, M. Morita, K. Hori and H. Unno

    J. Biosci. Bioeng   Vol. 94   page: 172-174   2002

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  118. Antimicrovial activity of a biocide (5-chloro-2-methyl-4-isothiazolin-3-one; CMI) on a biofilm Reviewed

    Y. Tanji, T. Nakano, K. Hori, K. Miyanaga and H. Unno

    Corrosion Engineering   Vol. 51   page: 777-785   2002

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  119. Chemical analysis of artificial biofilm that enhances or inhibits carbon steel corrosion Reviewed

    Y. Tanji, T. Itoh, T. Nakano, K. Hori and H. Unno

    Corrosion   Vol. 58   page: 232-239   2002

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  120. Fungal contribution to in situ biodegradation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) film in soil Reviewed

    B-I. Sang, K. Hori, Y. Tanji and H. Unno

    Appl. Microbiol. Biotechnol   Vol. 58   page: 241-247   2002

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  121. A new device for in situ sampling of dispersion for image analysis Reviewed

    K. Hori, M. Yamaji, Y. Tanji and H. Unno

    Meas. Sci. Technol   Vol. 13   page: N27-N29   2002

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  122. Behavior of filamentous cyanobacterium Anabaena spp. in water column and its cellular characteristics Reviewed

    32. K. Hori, S. Ishii, G. Ikeda, J. Okamoto, Y. Tanji, C. Weeraphasphong and H. Unno

    Biochem. Eng. J   Vol. 10   page: 217-225   2002

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  123. Simultaneous production of polyhydroxyalkanoates and rhamnolipids by Pseudomonas aeruginosa Reviewed

    K. Hori, Sidikmarsudi and H. Unno

    Biotechnol. Bioeng   Vol. 78   page: 699-707   2002

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  124. Isolation, characterization and application to off-gas treatment of toluene degrading bacteria Reviewed

    K. Hori, S. Yamashita, S. Ishii, M. Kitagawa, Y. Tanji and H. Unno

    J. Chem. Eng. Japan   Vol. 34   page: 1120-1126   2001

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  125. 木炭および生物木炭の水質浄化機能

    堀 克敏

    バイオサイエンスとインダストリー   Vol. 59   page: 27-30   2001

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  126. Addition of 2,4-D enhances excretion of barley lectin in transgenic tabacco cells of vacuole targeting signal deleted mutant Reviewed

    A. Ishihara, S. Wakao, Y. Tanji, X-H. Xing, K. Hori, K. Miyanaga and H. Unno

    Plant Biotechnology   Vol. 18   page: 27-31   2001

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  127. Seasonal changes of coliphage in septic tank sludge and quantification of P1 phage mediated gene transfer Reviewed

    Y. Tanji, K. Mizoguchi, M. Yanagida, K. Hori and H. Unno

    J. Chem. Eng. Japan   Vol. 34   page: 1079-1083   2001

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  128. A kinetic analysis of the fungal degradation process of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in soil Reviewed

    B-I. Sang, K. Hori, Y. Tanji and H. Unno

    Biochem. Eng. J   Vol. 9   page: 175-184   2001

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  129. Characterization of phage encoded lysis proteins and its application for cell disruption Reviewed

    Y. Tanji, K. Hori, S. Yamamoto and H. Unno

    Bioseparation Eng. (Progress in Biotechnol.)   Vol. 16   page: 207-211   2000

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  130. 微生物による環境浄化

    海野 肇, 堀 克敏

    化学と教育   Vol. 12   page: 812-815   2000

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  131. 化学プロセスにおける塩素フローの解析と塩素化合物量の低減

    海野 肇,丹治保典,堀 克敏

    ケミカルエンジニヤリング   Vol. 45   page: 69-77   2000

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  132. ポリ塩化ビニル製造における塩素フローの解析と塩素利用の適正化 Reviewed

    45. 丹治保典、堀 克敏、宮永一彦、海野 肇、けい新会

    環境科学会誌   Vol. 13   page: 556-561   2000

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  133. Microbial degradation kinetics of solid alkane dissolved in nondegradable oil phase Reviewed

    D. H. Choi, K. Hori, Y. Tanji and H. Unno

    Biochem. Eng. J   Vol. 3   page: 71-78   1999

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  134. Evidence for a novel Chlorella virus-encoded alginate lyase Reviewed

    K. Suda, Y. Tanji, K. Hori, H. Unno

    FEMS Microbiol. Lett   Vol. 180   page: 45-53   1999

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  135. Structural analysis of a biofilm which enhances carbon steel corrosion in nutritionally poor aquatic environments Reviewed

    Y. Tanji, Y. Morono, A. Soejima, K. Hori and H. Unno

    J. Biosci. Bioeng   Vol. 88   page: 551-556   1999

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  136. 木炭・微生物の複合機能による水質浄化

    堀 克敏

    ケミカルエンジニヤリング   Vol. 43   page: 29-33   1998

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  137. 有機塩素化合物の微生物分解

    堀 克敏

    生物工学会誌   Vol. 76   page: 437   1998

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  138. Effects of culture conditions on molecular weights of poly(3-hydroxyalkanoates) produced by Pseudomonas putida from octanoate Reviewed

    K. Hori, K. Soga and Y. Doi

    Biotechnology Letters   Vol. 16   page: 709-714   1994

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  139. Production of poly(3-hydroxybutyrate) from plant oils by Alcaligenes sp Reviewed

    M. I. A. Majid, K. Hori, M. Akiyama and Y. Doi

    Studies in Polymer Sci   Vol. 12   page: 417-424   1994

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  140. Production of Poly (3-hydroxyalkanoates-co-3-hydroxy-ω-fluoroalkanoates) by Pseudomonas oleovorans from 1-fluorononane and gluconate Reviewed

    K. Hori, K. Soga and Y. Doi

    Biotechnology Letters   Vol. 16   page: 501-506   1994

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  141. Isolation and sequence of a developmentally regulated putative novel gene, priA, from the basidiomycete Lentinus edodes Reviewed

    S. Kajiwara, K.Yamaoka, K. Hori, H. Miyazawa, T. Saito, T. Kannno and K. Shishido

    Gene   Vol. 114   page: 173-178   1992

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  142. Cloning, sequence analysis and transcriptional expression of a ras gene of the edible basidiomycete Lentinus edodes Reviewed

    K. Hori, S. Kajiwara, T. Saito, H. Miyazawa, Y. Katayose and K. Shishido

    Gene   Vol. 105   page: 91-96   1991

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Books 14

  1. 接着性被毛細菌のバイオフィルム形成 『バイオフィルム革新的制御技術』

    堀 克敏( Role: Joint author)

    エヌ・ティー・エス  2023.6 

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    Responsible for pages:115-123   Language:Japanese

  2. Comprehensive Biotechnology, Third Edition International journal

    K. Hori and H. Unno( Role: Contributor ,  Integrated Production and Separation)

    Academic Press  2019.1  ( ISBN:9780444640468

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    Responsible for pages:651-662   Language:English Book type:Textbook, survey, introduction

    Bioprocessing has been expected as a promising production tool for the new era. However, the processes have been confronted with various difficulties, mostly cost ineffectiveness. To overcome this weakness, the process integration has been proposed to increase the process economy and investigated intensively. In this article, the conceptual frame of the process integration is presented, and the integration methods are classified in view of bioprocess development. Successful combination of bioreactors with traditional separation techniques such as extraction, adsorption, etc., and application of membrane technology is overviewed. Several ideas for the integration by the use of biotechnological principle are also discussed.

    DOI: 10.1016/B978-0-444-64046-8.00132-4

    Scopus

  3. Biofilm and Materials Science International journal

    Katsutoshi Hori( Role: Contributor ,  Adhesion of Bacteria)

    Springer  2015  ( ISBN:978-3-319-14564-8

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    Total pages:196   Responsible for pages:23-34   Language:English Book type:Scholarly book

  4. 低コスト・ハイパフォーマンス技術による水処理革命

    堀 克敏,斎藤永宏,向井康人,上野智永,三浦篤人,中川勝統,BernhardWett,朝倉義幸,松山秀人,市野良一,佐伯大輔,神本祐樹,藤岡正剛,福崎康博,鈴村慶太郎,野村記生( Role: Supervisor (editorial) ,  有機排水の生物処理技術)

    コロナ社  2013.12  ( ISBN:978-4-339-06630-2

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    Total pages:242   Responsible for pages:2-40   Language:Japanese Book type:Scholarly book

  5. バイオテクノロジーシリーズ「細胞表層工学の進展」

    堀 克敏( Role: Contributor ,  バクテリオナノファイバーの細胞表層工学への応用)

    シーエムシー出版  2020.4  ( ISBN:978-4-7813-1496-9

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    Total pages:246   Responsible for pages:209-218   Language:Japanese Book type:Scholarly book

  6. 細胞・生体分子の固定化と機能発現

    堀 克敏( Role: Contributor ,  微生物ナノファイバーを用いた可逆的な微生物細胞固定化技術)

    シーエムシー出版  2018  ( ISBN:978-4-7813-1326-9

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    Total pages:294   Responsible for pages:161-171   Language:Japanese Book type:Scholarly book

  7. Reference Module in Life Sciences International journal

    K. Hori and H. Unno( Role: Contributor ,  Integrated production and separation)

    Elsevier  2017 

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    Responsible for pages:579-590   Language:English Book type:Textbook, survey, introduction

  8. 光合成のエネルギー利用と環境応用

    丁 楠,中谷 肇,堀 克敏( Role: Contributor ,  暗発酵による水素生産)

    シーエムシー出版  2014  ( ISBN:978-4-7813-0927-9

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    Total pages:305   Language:Japanese Book type:Scholarly book

  9. 次世代のバイオ水素エネルギー

    天尾 豊、三宅 淳、南後 守、堀 克敏、( Role: Joint author ,  バイオ水素エネルギー開発の新しい展開をめざして)

    化学同人  2014  ( ISBN:978-4-7598-1375-3

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    Total pages:174   Responsible for pages:2-11   Language:Japanese Book type:Scholarly book

  10. Integrated production and separation, in Comprehensive Biotechnology, E. Collins (ed.)

    Katsutoshi Hori, Hajime Unno( Role: Joint author)

    Elsevier  2011.6 

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    Language:English

  11. 付着機構の解明と工学的応用可能性 『バイオフィルムの基礎と制御』

    堀 克敏( Role: Joint author)

    エヌ・ティー・エス  2008 

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    Language:Japanese

  12. 環境をきれいにするタンパク質 『とことんやさしいタンパク質の本』

    堀 克敏( Role: Joint author)

    日刊工業新聞社  2007 

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    Language:Japanese

  13. 木炭および生物木炭の水質浄化機能 『微生物固定化法による水処理』

    堀 克敏( Role: Joint author)

    エヌ・ティー・エス  2000 

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    Language:Japanese

  14. 微生物ポリエステル 『生分解性プラスチックハンドブック』

    堀 克敏・土肥義治( Role: Joint author)

    エヌ・ティー・エス  1995 

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    Language:Japanese

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MISC 1

  1. 細菌接着タンパク質と微生物固定化への利用 Invited

    堀 克敏

    バイオインダストリー   Vol. 39   page: 11 - 18   2022

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

Presentations 446

  1. [受賞講演]細菌の接着機構と界面微生物工学のプロセスの創出に関する研究 Invited

    堀 克敏

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋   Country:Japan  

  2. [受賞講演]微生物接着と革新的固定化微生物プロセスの構築 Invited

    堀 克敏

    化学工学会第88年会  2023.3.15  公益社団法人 化学工学会

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    Event date: 2023.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東京   Country:Japan  

  3. Contribuition of the Fenton reaction to the CNT degradation by enzymes Invited International conference

    Katsutoshi Hori

    The 9th NanoCarbon Annual Conference  2024.3.20  Cluster Nanotechnology

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    Event date: 2024.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Wurzburg   Country:Germany  

  4. Long-term continuous CNT degradation by a bacteria-driven Fenton reaction Invited International conference

    Katsutoshi Hori

    The 9th NanoCarbon Annual Conference  2024.3.20  Cluster Nanotechnology

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    Event date: 2024.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Wurzburg   Country:Germany  

  5. A Novel Bacterionanofiber Adhesin and the Development of Innovative Bioprocesses Invited International coauthorship International conference

    Katsutoshi Hori

    2024 KSBB Spring Meeting and International Symposium  2023.4.18  The Korean Society for Biotechnology and Bioengineering

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    Event date: 2024.4

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Changwon   Country:Korea, Republic of  

  6. Acinetobacter sp. Tol5 のトルエン取り込みトランスポーターの探索と機能解析

    森さくら, 吉本将悟, 岡 大椰, 堀 克敏

    化学工学会第89年会  2024.3.18 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:大阪   Country:Japan  

  7. 人工タンパク質繊維に向けた繊維状タンパク質重合反応の基礎検討

    長谷彩紗、南畑孝介、石川聖人、吉本将悟、堀 克敏、神谷典穂

    化学工学会第89年会  化学工学会

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    Event date: 2024.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:大阪  

  8. 高活性油脂分解微生物を用いた食用油含有排水処理技術の確立と社会実装

    小原優季, 蟹江純一, 堀 克敏

    化学工学会第89年会  2024.3.18 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  9. 接着タンパク質AtaAの小型化による大腸菌の固定化と微生物反応

    吉本将悟, 堀 克敏

    化学工学会第89年会  2024.3.18 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  10. 三量体接着タンパク質AtaAドメインの力学的強靭性の分子メカニズム解析

    笹原 純, 加藤 周, 吉本将悟, 藤本和士, 堀 克敏

    化学工学会第89年会  2024.3.18 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:大阪   Country:Japan  

  11. 毛様タンパク質AtaAのファーバー基部の力学特性計測

    加藤 周, 笹原 純, 吉本将悟, 堀 克敏

    化学工学会第89年会  2024.3.18 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:大阪   Country:Japan  

  12. Long-term continuous degradation of carbon nanotubes by a bacteria-driven Fenton reaction

    2024.3.6 

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    Event date: 2024.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  13. Identification of the adhesive domain on a trimeric autotransporter adhesin and its application in immobilizing Escherichia coli Invited International conference

    K. Hori

    Asian Synthetic Biology Association 2023  2023.12.13  Asian Synthetic Biology Association

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    Event date: 2023.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Hyogo   Country:Japan  

  14. 単層CNTの生分解性に関する新たな知見 Invited

    堀 克敏

    NBCIビジネス委員会  2023.12.5  ナノテクノロジー推進協議会ナノカーボン実用化WG

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    Event date: 2023.12

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:東京  

  15. 社会実装のための生物物理学分野の研究戦略:-起業家的科学者からの洞察 Invited

    堀 克敏

    第61回日本生物物理学会  2023.11.14  一般社団法人 日本生物物理学会

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    Event date: 2023.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋   Country:Japan  

  16. Acinetobacter sp. Tol 5 の外膜タンパク質AtaAにおけるリシン残基のメチル化修飾

    井上 翔理、岡 大椰、吉本 将悟、堀 克敏

    化学工学会第54回秋季大会  2023.9.11  化学工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:福岡   Country:Japan  

  17. 分子動力学シミュレーションによる三量体接着タンパク質AtaAの力学的安定性の分子機構解析

    笹原 純、吉本 将悟、藤本 和士、堀克敏

    化学工学会第54回秋季大会  2023.9.11  化学工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:福岡   Country:Japan  

  18. 細胞表層タンパク質AtaAの膜貫通ドメインを用いたナノポアの構築

    黄泰賢, 吉本将悟, 笹原純, 川野竜司, 堀克敏

    化学工学会第54回秋季大会  2023.9.11  化学工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:福岡   Country:Japan  

  19. 原子間力顕微鏡と分子動力学計算による最近ファイバータンパク質AtaAの力学強度解析

    笹原 純、加藤 周、吉本 将悟、和士、堀 克敏

    第17回バイオ関連化学シンポジウム  2023.9.8  バイオ関連シンポジウム

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    Event date: 2023.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京   Country:Japan  

  20. 接着タンパク質AtaAの機能部位特定と小型AtaAを用いた大腸菌の迅速簡便な固定化 International coauthorship

    吉本 将悟、石川 聖人、鈴木 敦巨、Linke Dirk, Lupas Andrei, 堀 克敏

    第17回バイオ関連化学シンポジウム  2023.9.8  バイオ関連シンポジウム

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  21. ヘム酵素存在下でのカーボンナノチューブ分解へのフェントン反応の寄与

    高橋 慧良、田口 冨美子、堀 克敏

    第17回バイオ関連化学シンポジウム  2023.9.8  バイオ関連シンポジウム

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    Event date: 2023.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京   Country:Japan  

  22. Analysis of fish epidermal bacteriota for the development of novel disease control methods in aquaculture International conference

    H. Nakatani, N. Yamada, N. Hashimoto, F. Okazaki, T. Arakawa, K. Hori

    Chubu International Symposium for Biotechnology 2023  2023.9.6 

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    Event date: 2023.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  23. Identification of the adhesive domain of AtaA from Acinetobacter sp. Tol 5 and its application in immobilizing Escherichia coli International coauthorship International conference

    S. Yoshimoto, S. Aoki, Y. Ohara, M. Ishikawa, A. Suzuki, D. Linke, A. Lupas, K. Hori

    Chubu International Symposium for Biotechnology 2023  2023.9.6 

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    Event date: 2023.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  24. Development of a fast feature extraction method for SARS-CoV-2 spike sequences using amino acid physical properties International conference

    H. Oka, Y. Yoshimoto, K. Hori

    Chubu International Symposium for Biotechnology 2023  2023.9.6 

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    Event date: 2023.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  25. Functional analysis of the outer membrane transporter of the toluene-utilizing bacterium Acinetobacter sp. Tol 5 International conference

    S. Mori, S. Yoshimoto, H. Oka, K. Hori

    Chubu International Symposium for Biotechnology 2023  2023.9.6 

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    Event date: 2023.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  26. Development of whole-cell biocatalysts expressing exogenous carbonic anhydrase for efficient CO2 utilization International conference

    K. Abe, H. Oka, K. Hori

    Chubu International Symposium for Biotechnology 2023  2023.9.6 

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    Event date: 2023.9

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  27. Single-molecule toughness analysis of the trimeric adhesive protein AtaA using atomic microscopy International conference

    A. Kato, J. Sasahara, S. Yoshimoto, K. Hori

    Chubu International Symposium for Biotechnology 2023  2023.9.6 

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    Event date: 2023.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  28. Construction of a nanopore using the transmembrane domain of the cell surface protein AtaA International conference

    T. Hwang, S. Yoshimoto, J. Sasahara, R. Kawano, K. Hori

    Chubu International Symposium for Biotechnology 2023  2023.9.6 

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    Event date: 2023.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  29. Mechanical stability analysis of the trimeric adhesive protein AtaA by all-atom molecular dynamics simulation International conference

    J, Sasahara, S. Yoshimoto, K. Fujimoto, K. Hori

    Chubu International Symposium for Biotechnology 2023  2023.9.6 

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    Event date: 2023.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  30. Extensive lysine methylation of the outer membrane protein AtaA in Acinetbacter sp. Tol 5 International conference

    S. Inoue, H. Oka, S. Yoshimoto, K. Hori

    Chubu International Symposium for Biotechnology 2023  2023.9.6 

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    Event date: 2023.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  31. Experimental and computational approaches to the molecular characterization of a bacterial adhesin protein International conference

    K. Hori, J. Sasahara, A. Kato, S. Yoshimoto

    The 75th Annual Meeting of the Society for Biotechnology  2023.9.5 

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    Event date: 2023.9

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Venue:Nagoya   Country:Japan  

  32. Analysis of fish epidermal bacteriota for the development of novel disease control methods in aquaculture International coauthorship International conference

    H. Nakatani, F. Okazaki, T. Arakawa, D. Anggorowati, K. Anam, D. Susulaningsih, Y. Tamaru, K. Hori

    The 75th Annual Meeting of the Society for Biotechnology  2023.9.5 

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    Event date: 2023.9

    Language:English   Presentation type:Symposium, workshop panel (nominated)  

    Country:Japan  

  33. Degradation of carbon nanotubes by the Fenton reaction occurring in the presence of heme enzymes

    高橋 慧良、田口 冨美子、堀 克敏

    第65回フラーレン・ナノチューブ・グラフェン総合シンポジウム  2023.9.4 

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    Event date: 2023.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  34. アミノ酸物性値を用いた SARS-CoV-2 スパイク配列の高速特徴抽出法の開発

    岡 大椰、吉本 将悟、堀 克敏

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  35. 三量体接着タンパク質AtaAの小型化とそれを用いた大腸菌の固定化 International coauthorship

    吉本 将悟、青木 壮太、小原 優季、石川 聖人、鈴木 敦巨、Linke Dirk, Lupas Andrei, 堀 克敏

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  36. 膜内外に異種タンパク質を連結した、バクテリアサイズメラリポソームの簡便な作作製法

    野場 孝策、吉本 将悟、田中 良和、横山 武志、松浦 友亮、堀 克敏

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  37. A novel inverse membrane bioreactor for efficient bioconversion from methane gas to liquid methanol using a microbial gas-phase reaction

    Y-Y. Chen, M. Ishikawa, and K. Hori

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:English   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  38. 合成生物学利用に向けた形質転換効率の低い最近の遺伝子工学的改変

    石川 聖人、堀 克敏

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  39. 三量体型オートトランスポーターアドヘシンの膜貫通ドメインを用いたナノポアの構築

    黄 泰賢、吉本 将悟、笹原 純、川野 竜司、堀 克敏

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  40. 全原子分子動力学計算による三量体接着タンパク質AtaAの機械的安定性のメカニズムの解析

    笹原 純、吉本 将悟、藤本 和士、堀 克敏

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  41. 驚異的な油脂分解能力をもつ微生物による超効率的な食用油含有排水処理法の確立と社会的実装

    小原 優季、蟹江 純一、堀 克敏

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  42. トルエン質化最近Acinetobacter sp. Tol 5 の外膜トランスポーター機能解析

    森 さくら、吉本 将悟、岡 大椰、堀 克敏

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  43. トルエン質化最近Acinetobacter sp. Tol 5 の外膜タンパク質におけるメチル化修飾

    井上 翔理、岡 大椰、吉本 将悟、堀 克敏

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  44. 繊維状タンパク質の量末端連結反応を基礎とした人工繊維の形成

    長谷 彩沙、南畑 孝介、石川 聖人、吉本 将悟、堀 克敏、神谷 典穂

    第75回日本生物工学会大会  2023.9.3  日本生物工学会

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    Event date: 2023.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  45. Single-cell adhesion force mapping of a highly sticky bacterium in liquid International conference

    K. Hori, S. Yoshimoto

    The 10th FEMS Congress of European Microbiologists  2023.7.9  Federation of European Microbiological Societies

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    Event date: 2023.7

    Language:English   Presentation type:Poster presentation  

    Venue:Hamburg   Country:Germany  

  46. Mechanical stability analysis of an Acinetobacter adhesin by steered molecular dynamics simulation International coauthorship International conference

    J. Sasahara, K. Fujimoto, S. Yoshimoto, D. Linke, A. Lupas, K. Hori

    The 10th FEMS Congress of European Microbiologists  2023.7.9  Federation of European Microbiological Societies

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    Event date: 2023.7

    Language:English   Presentation type:Poster presentation  

    Venue:Hamburg   Country:Germany  

  47. Immobilization of biocatalysts on solid surfaces using the bacterionanofiber protein AteA International conference

    S. Yoshimoto, M. Sawada, K. Noba, M. Ishikawa, K. Hori

    The 10th FEMS Congress of European Microbiologists  2023.7.9  Federation of European Microbiological Societies

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    Event date: 2023.7

    Language:English   Presentation type:Poster presentation  

    Venue:Hamburg   Country:Germany  

  48. Acinetobacter sp.Tol5の外膜タンパク質AtaAにおけるメチル化修飾の解析

    井上 翔理、岡 大椰、吉本 将悟、堀 克敏

    生物工学若手研究者の集い 夏のセミナー2023  2023.6.24  日本生物工学会

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    Event date: 2023.6

    Language:Japanese   Presentation type:Poster presentation  

    Venue:富山   Country:Japan  

  49. 組換え体膜タンパク質を用いたナノポアの創出と特性解析

    黄 泰賢、吉本 将悟、笹原 純、川野 竜司、堀 克敏

    生物工学若手研究者の集い 夏のセミナー2023  2023.6.24  日本生物工学会

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    Event date: 2023.6

    Language:Japanese   Presentation type:Poster presentation  

    Venue:富山   Country:Japan  

  50. 細胞表層タンパク質AtaA_Cheadのナノ力学応答解析

    笹原 純、加藤 周、橋本 貴幸、吉本 将悟、藤本 和士、堀 克敏

    生物工学若手研究者の集い 夏のセミナー2023  2023.6.24  日本生物工学会

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    Event date: 2023.6

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  51. 「環境にあってはならない物質」に依存させる生物学的封じ込め Invited

    堀 克敏、石川 聖人

    日本農芸化学会2022年度大会  2023.3.16  日本農芸化学会

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    Event date: 2023.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:オンライン   Country:Japan  

  52. Bottom-up Creation of an Artificial Cell Covered with the Adhesive Bacterionanofiber Protein A ta A International conference

    K. Noba, M. Ishikawa, A. Uyeda, T. Watanabe, T. Hohsaka, S. Yoshimoto, T. Matsuura, K. Hori

    MISC symposium in Oslo 2023  2023.2 

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    Event date: 2023.2

    Language:English   Presentation type:Poster presentation  

    Venue:Oslo   Country:Norway  

  53. Application of a bacterial adhesin to bioprocesses Invited International conference

    K. Hori

    MISC symposium in Oslo 2023  2023.2 

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    Event date: 2023.2

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Oslo   Country:Norway  

  54. Analysis of outer membrane protein methylation in Acinetobacter sp.Tol 5 International conference

    S. Inoue, H. Oka, S. Yoshimoto, K. Hori

    MISC symposium in Oslo 2023  2023.2 

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    Event date: 2023.2

    Language:English   Presentation type:Poster presentation  

    Venue:Oslo   Country:Norway  

  55. Bacterial degradation of trimethylamine International conference

    S. Muto, H. Oka, K. Hori

    MISC symposium in Oslo 2023  2023.2 

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    Event date: 2023.2

    Language:English   Presentation type:Poster presentation  

    Venue:Oslo   Country:Norway  

  56. Construction of β-barrel nanopores utilizing the transmembrane domain of a trimeric autotransporter adhesin International conference

    T. Hwang, S. Yoshimoto, K. Hori

    MISC symposium in Oslo 2023  2023.2 

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    Event date: 2023.2

    Language:English   Presentation type:Poster presentation  

    Venue:Oslo   Country:Norway  

  57. Mechanical stability analysis of a cell surface protein AtaA_Chead by steered molecular dynamics simulation and atomic force microscopy International conference

    J. Sasahara, K. Fujimoto, T. Hashimoto, A. Kato, S. Yoshimoto, K. Hori

    MISC symposium in Oslo 2023  2023.2 

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    Event date: 2023.2

    Language:English   Presentation type:Poster presentation  

    Venue:Oslo   Country:Norway  

  58. バイオコントロールとバイオインターフェイスをベースとした革新的微生物技術による起業 Invited

    堀 克敏

    第74回日本生物工学会大会  2022.10.20  公益社団法人 日本生物工学会

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    Event date: 2022.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:オンライン   Country:Japan  

  59. 序章:非特異的接着性を示すバクテリオナノファイバータンパク質AtaA Invited

    堀 克敏

    第74回日本生物工学会大会  2022.10.18  公益社団法人 日本生物工学会

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    Event date: 2022.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:オンライン   Country:Japan  

  60. 気相微生物反応 Invited

    堀 克敏

    第60回日本生物物理学会年会  2022.9.30  一般社団法人 日本生物物理学会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:北海道   Country:Japan  

  61. MD計算を用いた細胞表層タンパク質AtaA_Cheadの力学応答解析

    笹原 純、橋本 貴幸、藤本 和士、吉本 将悟、堀 克敏

    2022年度日本生物工学会中部支部例会  2022.9.22  日本生物工学会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  62. The Acinetobacter trimeric autotransporter adhesin AtaA showing non-specific and strong adhesiveness and its application to bioprocess Invited

    2022.9.22 

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    Event date: 2022.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  63. 原子間力顕微鏡を用いた高付着性細菌の1細胞・1分子付着力解析

    吉本 将悟、堀 克敏

    第16回バイオ関連化学シンポジウム  2022.9.11  日本化学会生体機能関連化学部会、日本化学会バイオテクノロジー部会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  64. 原子間力顕微鏡を用いたAtaAのC末端パッセンジャードメインの1分子強靭性解析

    加藤 周,吉本 将悟,堀 克敏

    第16回バイオ関連化学シンポジウム  2022.9.11  日本化学会生体機能関連化学部会、日本化学会バイオテクノロジー部会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋   Country:Japan  

  65. 人口細菌を志向した非対称なタンパク質構成を有する細菌サイズのリポソーム創出

    野場 孝策、吉本 将悟、田中 良和、松浦 友亮、堀 克敏

    第16回バイオ関連化学シンポジウム  2022.9.11  日本化学会生体機能関連化学部会、日本化学会バイオテクノロジー部会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋   Country:Japan  

  66. 分子動力学シミュレーションによる細胞表層タンパク質AtaA_Cheadの力学応答解析

    笹原 純、橋本 貴幸、藤本 和士、吉本 将悟、堀 克敏

    第16回バイオ関連化学シンポジウム  2022.9.11  日本化学会生体機能関連化学部会、日本化学会バイオテクノロジー部会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋   Country:Japan  

  67. プラスチックの分解速度向上を目指した酵素複合体の構築

    澤田 美波、吉本 将悟、堀 克敏

    第16回バイオ関連化学シンポジウム  2022.9.11  日本化学会生体機能関連化学部会、日本化学会バイオテクノロジー部会

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    Event date: 2022.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋   Country:Japan  

  68. CNT degradation by bacterial enzymes Invited International conference

    K. Hori

    NanoCarbon Annual Conference 2022  2022.6.22 

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    Event date: 2022.6

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Wurzburg   Country:Germany  

  69. CNT degradation by bacterial enzymes International conference

    K. Hori

    The 22nd International Conference on the Science and Applications of Nanotubes and Low-Dimensional Materials  2022.6.24 

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    Event date: 2022.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  70. Functionalization of cycloparaphenylene by biocatalysts

    K. Hori , A. Usami, K. Amaike, K. Itami

    2022.3.26 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  71. 接着性被毛細菌の細胞接着・バイオフィルム形成機構と応用例 Invited

    堀 克敏

    発酵と代謝研究会  2022.3.14  一般財団法人 バイオインダストリー協会

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    Event date: 2022.3

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:オンライン   Country:Japan  

  72. Non-enzymatic degradation of carbon nanotubes in the presence of bacterial enzymes

    K. Hori , S. Takahashi, F. Taguchi, J. Kanie, M.Ueno, M.Uejima, M. Ata

    2022.3.2 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  73. Investigation of single-walled carbon nanotubes degradation by hemeenzymes from soil bacteria

    S. Takahashi, F. Taguchi, J. Kanie, M.Ueno, M.Uejima, M. Ata, K. Hori

    2022.3.2 

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    Event date: 2022.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  74. Application of a bacterionanofiber protein to cell surface display engineering Invited International conference

    K. Hori

    The 2021 International Chemical Congress of Pacific Basin Societies  2021.12.17  Pacifichem

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    Event date: 2021.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Online   Country:United States  

  75. Adhesion mapping of a highly adhesive bacterium using atomic force microscopy International conference

    S. Yoshimoto, K. Hori

    The 2021 International Chemical Congress of Pacific Basin Societies  2021.12.17  Pacifichem

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    Event date: 2021.12

    Language:English   Presentation type:Poster presentation  

    Venue:Online   Country:United States  

  76. Investigation of single-walled carbon nanotubes degradation by heme enzymes from soil bacteria International conference

    S. Takahashi, F. Taguch, J. Kanie, K. Hori

    The 2021 International Chemical Congress of Pacific Basin Societies  2021.12.17  Pacifichem

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    Event date: 2021.12

    Language:English   Presentation type:Poster presentation  

    Country:United States  

  77. Molecular dynamics simulation of adhesion process of a highly adhesive protein AtaA's N-terminal domain in water International conference

    J. Sasahara, A. Suzuki, K. Fujimoto, S. Okazaki, K. Hori

    The 2021 International Chemical Congress of Pacific Basin Societies  2021.12.17  Pacifichem

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    Event date: 2021.12

    Language:English   Presentation type:Poster presentation  

    Venue:Online   Country:United States  

  78. Bottom-up creation of an artificial cell covered with the adhesive bacterionanofiber protein AtaA International conference

    K. Noba, M. Ishikawa, A. Uyeda, T. Watanabe, T. Hohsaka, S. Yoshimoto, T. Matsuura, K. Hori

    The 2021 International Chemical Congress of Pacific Basin Societies  2021.12.17  Pacifichem

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    Event date: 2021.12

    Language:English   Presentation type:Poster presentation  

    Venue:Online   Country:United States  

  79. ユニークな細菌接着タンパク質とその工学利用 Invited

    堀 克敏

    微生物コンソーシアム G2 第10回定例会  2021.12.7  大隅基礎科学創成財団

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    Event date: 2021.12

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:オンライン   Country:Japan  

  80. 魚類養殖におけるプロバイオティクス飼料開発

    山田 紗里奈、岡﨑 文美、梅川 碧里、中谷 肇、堀 克敏、田丸 浩

    第73回日本生物工学会大会  2021.10.29  日本生物工学会

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    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  81. 長鎖反復配列遺伝子の相同組換え頻度の解析

    石川 聖人、堀 克敏

    第73回日本生物工学会大会  2021.10.28  日本生物工学会

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    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  82. サケ・マス類のレッドマウス病原因菌 Yersinia ruckeriの経皮感染モデルの構築

    中谷 肇、堀 克敏

    第73回日本生物工学会大会  2021.10.29  日本生物工学会

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    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  83. がん細胞の柔軟化に関わる中間径フィラメントネスチンのテール領域とアクチン繊維の力学的相互作用解析

    山岸 彩奈、内田 幸希、上田 太郎、吉本 将悟、堀 克敏,中村 史

    第73回日本生物工学会大会  2021.10.28  日本生物工学会

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    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  84. 分子動力学的解析による高接着タンパク質の水中接着配向推定

    笹原 純、鈴木 淳巨、藤本 和士、岡崎 進、堀 克敏

    第73回日本生物工学会大会  2021.10.29  日本生物工学会

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    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  85. 材料表面の違いに応じた高接着タンパク質の接着挙動変化のMD計算による解析

    笹原 純、鈴木 淳巨、藤本 和士、岡崎 進、堀 克敏

    日本化学会秋季事業第11回CSJ化学フェスタ2021  2021.10.19  日本化学会

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    Event date: 2021.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  86. バクテリア酵素によるカーボンナノチューブのフェントン反応分解

    高橋 慧良、田口 冨美子、蟹江 純一、堀 克敏

    化学工学会第52回秋季大会  2021.9.24  化学工学会

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    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン   Country:Japan  

  87. 気相微生物反応におけるMethylococcus capsulatus str.Bathの代謝動態の変化

    石川 聖人、Chen Yan-yu, 相馬 悠希、高橋 政友、和泉 自泰、馬場 健史、堀 克敏

    化学工学会第52回秋季大会  2021.9.24  化学工学会

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  88. ニジマス表皮細菌叢の変遷に関わる特定の細菌種

    堀 克敏

    日本水産学会 令和3年度秋季大会  2021.9.15  日本水産学会

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  89. 高付着性細菌の定量的付着力解析

    吉本 将悟、堀 克敏

    第15回バイオ関連化学シンポジウム  2021.9.8  日本化学会バイオテクノロジー部会

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    Event date: 2021.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  90. EPT加水分解酵素と高付着性タンパク質の連結体構築

    澤田美波,吉本将悟,堀 克敏

    第15回バイオ関連化学シンポジウム  2021.9.8  日本化学会バイオテクノロジー部会

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    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン   Country:Japan  

  91. AFMを用いたファイバータンパク質AtaAの力学特性解析法の確立

    鈴木 浩平、吉本 将悟、堀 克敏

    第15回バイオ関連化学シンポジウム  2021.9.8  日本化学会バイオテクノロジー部会

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    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン   Country:Japan  

  92. 分子動力学計算に基づいた高接着タンパク質の材料表面との相互作用解析

    笹原 純、鈴木 淳巨、藤本 和士、岡崎 進、堀 克敏

    第15回バイオ関連化学シンポジウム  2021.9.8  日本化学会バイオテクノロジー部会

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    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン   Country:Japan  

  93. タンパク質の配向を制御した再構成型リポソームの作製

    野場 孝策、吉本 将悟、堀 克敏

    第15回バイオ関連化学シンポジウム  2021.9.8  日本化学会バイオテクノロジー部会

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    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン   Country:Japan  

  94. 細菌のヘム酵素が引き起こすフェントン反応によるカーボンナノチューブの分解

    高橋 慧良、田口 冨美子、蟹江 純一、堀 克敏

    第15回バイオ関連化学シンポジウム  2021.9.8  日本化学会バイオテクノロジー部会

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    Event date: 2021.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:オンライン   Country:Japan  

  95. バクテリア酵素存在下におけるカーボンナノチューブの分解

    高橋 慧良、堀 克敏

    2021年度日本生物工学会中部支部例会  2021.8.6  日本生物工学会中部支部

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    Event date: 2021.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  96. MD計算を用いた疎水・親水性表面に対する高接着タンパク質の接着挙動変化解析

    笹原 純、鈴木 淳巨、藤本 和士、堀 克敏

    2021年度日本生物工学会中部支部例会  2021.8.6  日本生物工学会中部支部

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    Event date: 2021.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  97. 分子動力学計算による高接着タンパク質の材料表面への接着過程解析

    笹原 純、鈴木 淳巨、藤本 和士、岡崎 進、堀 克敏

    生物工学会若手研究者の集い夏のオンラインセミナー  2021.7.17  日本生物工学会若手の会

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    Event date: 2021.7

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  98. Establishment of a percutaneous infection model using zebrafish and Yersinia ruckeri, a fish pathogen causing enteric redmouth International conference

    H. Nakatani, K. Hori

    2021 Sakura-Bio Meeting  2021.5.29 

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    Event date: 2021.5

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Online   Country:Japan  

  99. The trimeric autotransporter adhesin of the highly adhesive Acinetobacter strain Tol 5 Invited

    K. Hori, S. Yoshimoto

    2021.3.23 

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    Event date: 2021.3

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  100. ウイルス除去効果の高い3Dマスクの開発

    堀 克敏、小原 優季、蟹江 純一

    化学工学会第86年会  2021.3.21  化学工学会

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  101. フィルター交換型3Dマスクの開発 Invited

    堀 克敏

    不織布研究会・ナノファイバー研究会 合同研究会  2020.12.2  不織布研究会

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    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:オンライン   Country:Japan  

  102. 微生物の毛の機能と応用展開 Invited

    堀 克敏

    大隅ライフサイエンス研究会第4回シンポジウム  2020.11.27  大隅ライフサイエンス研究会

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    Event date: 2020.11

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

    Venue:オンライン   Country:Japan  

  103. 分子動力学計算を用いた細菌表層テザータンパク質の力学応答解析

    橋本 貴幸、笹原 純、吉本 将悟、堀 克敏

    生物工学会若手研究者の集い第三回オンラインセミナー  2020.11.21  日本生物工学会若手の会

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  104. 高接着タンパク質の疎水性表面への接着過程に関する分子動力学的解析

    笹原 純、鈴木 淳巨、藤本 和士、岡崎 進、堀 克敏

    生物工学会若手研究者の集い第三回オンラインセミナー  2020.11.21  日本生物工学会若手の会

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    Event date: 2020.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  105. 魚類表皮プロバイオティクス技術の創生

    堀 克敏

    生物工学会Webシンポジウム  2020.9.3  日本生物工学会

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    Event date: 2020.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:オンライン   Country:Japan  

  106. ニジマス表皮細菌叢の解析

    山田 尚毅,、中谷 肇,、荒川 友子、 堀 克敏

    令和2年度日本水産学会春季大会  日本水産学会

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  107. 正確なドメイン欠損による巨大ファイバータンパク質の再構成

    青木壮太, 吉本将悟, 石川聖人, 堀克敏

    化学工学会第85年会  2020.3.16  化学工学会

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  108. 環境汚染物質に依存する生物学的封じ込め技術の開発

    石川 聖人、堀 克敏

    化学工学会第85年会  2020.3.16  化学工学会

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  109. 発現ホストの違いによる高凝集性タンパク質AtaAの付着凝集特性の相違

    岩崎 啓太、吉本 将悟、堀 克敏

    化学工学会第85年会  2020.3.16  化学工学会

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  110. ゼブラフィッシュへのYersinia ruckeri経皮感染における表皮細菌叢の影響

    中谷 肇、塚本 裕也、堀 克敏

    令和2年度日本魚病学会春季大会  2020.3.7  日本魚病学会

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    Event date: 2020.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  111. Inhibition and detachment of AtaA-mediated bacterial adheasion by casein hydrolysates Invited International conference

    Y. Ohara, S. Yoshimoto, K. Hori

    The 25th Young Asian Biological Engineers' Community  2019.11.23 

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    Event date: 2019.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  112. Adheasion force mapping of cell surface of high adhesive bacteria using atomic force microscopy Invited International conference

    S. Ishii, S. Yoshimoto, K. Hori

    The 25th Young Asian Biological Engineers' Community  2019.11.23  Young Asian Biological Engineers' Community

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    Event date: 2019.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Korea, Republic of  

  113. The innovative wastewater treatment system from food industry using symbiotic microorganisms for oil degradation Invited International conference

    K. Hori

    The 10th Symposium on (iBioT2019)  2019.11.8  Innovative Bioproduction

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    Event date: 2019.11

    Language:English   Presentation type:Oral presentation (keynote)  

    Venue:Taichung, Taiwan   Country:Taiwan, Province of China  

  114. Alternation of mammalian circadian clock by extracellular electron transfer mediated by a cytocompatible redox polymer Invited International conference

    M. Ishikawa, K. Hori

    The 10th Symposium on Innovative Bioproduction Taichung (iBioT2018)  2019.11.8  Innovative Bioproduction

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    Event date: 2019.11

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Taichung   Country:Taiwan, Province of China  

  115. Acinetobacter sp. Tol 5 のcitral生物変換に関わる代謝経路の同定

    宇佐見享嗣, 石川聖人, 堀克敏

    第29回イソプレノイド研究会例会  2019.10.26  イソプレノイド研究会

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    Event date: 2019.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:沖縄   Country:Japan  

  116. バクテリオナノファイバー蛋白質AtaAで修飾した接着性人工細胞の創出

    野場考策, 石川聖人, 植田淳子, 渡邉貴嘉, 芳坂貴弘, 吉本将悟, 松浦友亮, 堀克敏

    日本化学会秋季事業 第9回CSJ化学フェスタ2019  2019.10.15  日本化学会

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    Event date: 2019.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  117. 分子動力学計算による高接着性タンパク質AtaA_Nheadの接着過程解析

    笹原純, 鈴木淳巨, 藤本和士, 岡崎 進, 堀克敏

    日本化学会秋季事業 第9回CSJ化学フェスタ2019  2021.9.15  日本化学会

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    Event date: 2019.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  118. A new aspect of the translocator complex of trimeric autotransporter adhesins of gram-negative bacterial, talking with membranes Invited International conference

    K. Hori

    biogenesis, communications and diseases, Beijing  2019.10.9 

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    Event date: 2019.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Beijing   Country:China  

  119. Genetic engineering of methylococcus capsulatus (bath) toward an efficient methane-based bioconversion International conference

    M. Ishikawa, K. Hori

    The 18th Asian Pacific Confederation of Chemical Engineering Congress  Asian Pacific Confederation of Chemical Engineering Congress

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    Event date: 2019.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  120. Development of a real gas-solid system for methane degradation with a novel cell-immobilization method International conference

    Y. Chen, M. Ishikawa, R. Suzuki, S. Yokoe, H. Ito, T. Kamachi, K. Hori

    The 18th Asian Pacific Confederation of Chemical Engineering Congress  Asian Pacific Confederation of Chemical Engineering Congress

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    Event date: 2019.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  121. The innovative wastewater treatment system from food industry using symbiotic microorganisms for oil degradation Invited International conference

    K. Hori

    The 18th Asian Pacific Confederation of Chemical Engineering Congress  Asian Pacific Confederation of Chemical Engineering Congress

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    Event date: 2019.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  122. Comparison of the adhesiveness of Acinetobacter sp. Tol 5 among various material surfaces International conference

    A.Kawashiri, S.Yoshimoto, T.Matsushita, K.Iio, M.Takai, K.Hori

    The 18th Asian Pacific Confederation of Chemical Engineering Congress  Asian Pacific Confederation of Chemical Engineering Congress

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    Event date: 2019.9

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  123. AFMを用いた高付着性細菌及びその細胞表層タンパク質の接着力測

    石井慧,吉本将悟,堀克敏

    第71回日本生物工学会大会  日本生物工学会

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山   Country:Japan  

  124. 環境汚染物質依存性の生物学的封じ込めシステムの開発

    石川聖人,飯尾魁,堀克敏

    第71回日本生物工学会大会 

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岡山   Country:Japan  

  125. Analysis of epidermal bacterial flora of fish: basic knowledge for probiotics Invited International conference

    K. Hori

    Marine Biotechnology Conference 2019 

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    Event date: 2019.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  126. Analysis of the skin bacterial flora of trout in fish farm International conference

    H.Nakatani., Y.Tsukamoto., N.Yamada,T. Arakawa, & K. Hori

    Marine Biotechnology Conference 2019 

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    Event date: 2019.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  127. バクテリオナノファイバー蛋白質AtaAで修飾した接着性人工細胞の創出

    野場考策,石川聖人,植田淳子,渡邉貴嘉,芳坂貴弘,吉本将悟,松浦友亮,堀克敏

    第13回バイオ関連化学シンポジウム  2019.9.4  日本化学会バイオテクノロジー部会

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    Event date: 2019.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮城   Country:Japan  

  128. 高付着性蛋白質AtaAで被覆された人工細胞のボトムアップ創成

    野場考策,石川聖人,植田淳子,渡邊貴嘉,芳坂貴弘,吉本将悟,松浦友亮,堀克敏

    2019年度 生物工学会中部支部例会  日本生物工学会中部支部

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    Event date: 2019.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岐阜   Country:Japan  

  129. Green bioproduction of monoterpenoid (E)-geranic acid from geraniol by genetically engineered Acinetobacter sp. Tol 5 in gas phase International conference

    A. Usami, M. Ishikawa, K. Hori

    FEMS 2019, 8th Congress of European Microbiologists  2019.7.9  FEMS

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    Event date: 2019.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Scotland   Country:United Kingdom  

  130. Geraniol dehydrogenaseの異種発現によるAcinetobacter sp.Tol5のcitral生物変換に関わる代謝経路の同定

    宇佐見享嗣、石川聖人、堀克敏

    日本農芸化学会2019年度大会  2019.3.24 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

  131. 高付着性細菌Acinetobacter sp. Tol 5の難付着性材料に対する付着性の評価

    川尻彩音,吉本将悟,松下大志,飯尾 魁,高井まどか,堀克敏

    化学工学会第84年会 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  132. 高付着性微生物及び表層タンパク質の原子間力顕微鏡解析

    石井 慧,吉本将悟,堀克敏

    化学工学会第84年会 

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    Event date: 2019.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  133. Development of an efficient counterselection method for Methylococcus capsulatus (bath) International conference

    M. Ishikawa, K. Hori

    Asian Synthetic Biology Association 2018 

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    Event date: 2018.11

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  134. Protein engineering of bacterionanofiber protein for the innovative bioprocesses Invited International conference

    Katsutoshi Hori

    Asian Synthetic Biology Association 2018 

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    Event date: 2018.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Korea, Republic of  

  135. Application of the adhesive bacterionanofiber protein AtaA to bioprocesses International conference

    Y. Ohara, S. Yoshimoto, K. Hori

    iBio-N 2018 

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    Event date: 2018.11

    Language:English   Presentation type:Poster presentation  

    Country:China  

  136. Distant variable peptide display system on bacterial cell utilizing long bacterionanofiber of a trimeric autotransporter adhesin International conference

    H. Nakatani, K. Hori

    iBio-N 2018 

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    Event date: 2018.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:China  

  137. Searching inhibitors against adhesion of the nanofiber protein AtaA and the control of microbial immobilization International conference

    Y. Ohara, S. Yoshimoto, K. Hori

    iBio-N 2018 

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    Event date: 2018.11

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Nanjing   Country:China  

  138. 接着ナノファイバー蛋白質AtaAの利用と分泌機構の理解 Invited

    堀 克敏、石川聖人

    化学工学会第50回秋季大会 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  139. AFMを用いた高付着性蛋白質AtaA及びAtaA被毛微生物の接着特性解析

    石井慧,吉本将悟,堀克敏

    第12回バイオ関連化学シンポジウム  2018.9.9 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪大学   Country:Japan  

  140. 水中強接着性細菌タンパク質AtaAの非特異的相互作用の謎に迫る Invited

    堀 克敏

    第70回日本生物工学会大会  2018.9.7 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  141. 両端をβドメインで終端したCoiled-Coilをベースとするランタニド結合タンパク質の設計

    鈴木淳巨,北原知恵,堀 克敏

    第70回日本生物工学会大会  2018.9.6 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:関西大学   Country:Japan  

  142. メタン酸化細菌Methlylococcus capsulatus (Bath)の効率的なカウンターセレクション法の開発

    石川聖人, 横江奨, 加藤創一郎,堀 克敏

    第70回日本生物工学会大会  2018.9.5 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:関西大学   Country:Japan  

  143. 高接着性蛋白質AtaAの反復配列を利用した部分欠損体の構築と機能ドメイン探索

    青木壮太、吉本将悟、石川聖人、堀 克敏

    第70回日本生物工学会大会  2018.9.5 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:関西大学   Country:Japan  

  144. Acinetobacter sp. Tol 5の難付着性材料に対する付着性の評価

    川尻彩音,飯尾 魁,吉本将悟,堀 克敏

    第70回日本生物工学会大会  2018.9.7 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:関西大学   Country:Japan  

  145. ナノファイバータンパク質AtaAの塩濃度依存的な接着性を利用した細菌の可逆的固定化

    吉本将悟,小原優季,中谷 肇,堀 克敏

    第70回日本生物工学会大会  2018.9.7 

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    Event date: 2018.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:関西大学   Country:Japan  

  146. 接着性バクテリオナノファイバーによる微生物プロセスの革新 Invited

    堀 克敏

    環境バイオテクノロジー学会2018年度大会 

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    Event date: 2018.6

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  147. Application of the adhesive bacterionanofiber protein AtaA to bioprocesses Invited International conference

    K. Hori

    China-Japan Joint Workshop of Frontiers of Systems Biotechnology and Biomanufacturing 

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    Event date: 2018.4

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:China  

  148. 遺伝子組換えAcinetobacter sp. Tol 5を用いたcitralの生物変換に関わる代謝経路の特定

    宇佐美亨嗣,石川聖人,堀克敏

    日本農芸化学会  2018.3.17 

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    Event date: 2018.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名城大学   Country:Japan  

  149. 油分解微生物を使った革新的排水処理技術 Invited

    堀 克敏

    第6回排水処理技術セミナー 

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    Event date: 2017.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  150. Extracellular production of a trimeric autotransporter adhesin from Acinetobacter sp. Tol 5 International conference

    T. Karakama, M. Ishikawa, S. Ishii, S. Yoshimoto, K. Hori

    IGER International Symposium on Cell Surface Structures and Functions 2017 

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    Event date: 2017.11 - 2017.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  151. Features on the structure and functions of the highly adhesive trimeric autotransporter adhesin AtaA from Acinetobacter sp. Tol 5 International conference

    K. Hori

    IGER International Symposium on Cell Surface Structures and Functions 2017 

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    Event date: 2017.11 - 2017.12

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  152. Structural insight into the highly adhesive protein AtaA International conference

    S. Yoshimoto, S. Aoki, J. Bassler, M. Ishikawa, A. Suzuki, M. Hartmann, D. Linke, A. Lupas, K. Hori

    IGER International Symposium on Cell Surface Structures and Functions 2017 

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    Event date: 2017.11 - 2017.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  153. Counting of the number of trimeric autotransporter adhesin molecules on the cell surface via SNAP-tag labeling International conference

    J. Kanie, Y. Matsuzaki, H. Nakatani, K. Hori

    IGER International Symposium on Cell Surface Structures and Functions 2017 

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    Event date: 2017.11 - 2017.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  154. Exploration of the minimum functional unit for elucidating the adhesion mechanism of AtaA International conference

    S. Aoki, S. Yoshimoto, M. Ishikawa, K. Hori

    IGER International Symposium on Cell Surface Structures and Functions 2017 

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    Event date: 2017.11 - 2017.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  155. The bacterial adhesion through adhesive nanofiber protein AtaA is inhibited and detached by casamino acid International conference

    Y. Ohara, S. Yoshimoto, S. Ishii, K. Hori

    IGER International Symposium on Cell Surface Structures and Functions 2017 

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    Event date: 2017.11 - 2017.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  156. A novel periplasmic protein TpgA forms a stable complex with a trimeric autotransporter adhesion International conference

    R. Hiroshige, S. Yoshimoto, A. Suzuki, M. Ishikawa, K. Hori

    IGER International Symposium on Cell Surface Structures and Functions 2017 

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    Event date: 2017.11 - 2017.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  157. Stickiness evaluation of the highly adhesive bacterium Acinetobacter sp. Tol 5 using atomic force microscopy International conference

    S. Ishii, S. Yoshimoto, K. Hori

    IGER International Symposium on Cell Surface Structures and Functions 2017 

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    Event date: 2017.11 - 2017.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  158. 付着性・凝集性細菌の付着・バイオフィルム形成過程と固定化技術の発展 Invited

    堀 克敏

    第49回化学工学会度秋季大会 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  159. SNAP-tag融合AtaAタンパク質の細胞表層提示条件の検討 Invited

    松崎由紀、蟹江純一、中谷 肇、堀 克敏

    第49回化学工学会度秋季大会 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  160. 細胞内レドックス状態の制御に基づく哺乳動物細胞の概日時計操作

    河合和紀、石川聖人、金子真大、中西周次、堀 克敏

    第69回日本生物工学会大会  2017.9.12 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:早稲田大学   Country:Japan  

  161. 微生物触媒の固定化を目指したナノファイバー蛋白質AtaAの小型化

    青木壮太、吉本将悟、堀 克敏

    第69回日本生物工学会大会  2017.9.13 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:早稲田大学   Country:Japan  

  162. ホモ三量体ファイバータンパク質AtaAの菌体外分泌生産の検討

    唐鎌智也、石川聖人、石井慧、吉本将悟、堀 克敏

    第69回日本生物工学会大会  2017.9.12 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:早稲田大学   Country:Japan  

  163. AFMを用いた高付着性細菌Tol 5の力学特性解析

    石井慧,吉本将悟,堀克敏

    第11回バイオ関連化学シンポジウム  2017.9.7 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  164. Acinetobacter sp. Tol 5 による citral の位置立体選択的生物変換と自然酸化

    宇佐見享嗣,石川聖人,堀克敏

    第11回バイオ関連化学シンポジウム  2017.9.8 

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    Event date: 2017.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  165. Quantification of cell adhesion of Acinetobacter sp. Tol 5 via a trimeric autotransporter adhesin using atomic force microscopy, International conference

    S. Ishii, S. Yoshimoto, K. Hori

    FEMS 7th Congress of European Microbiologists 

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    Event date: 2017.7

    Language:English   Presentation type:Poster presentation  

    Venue:Valencia   Country:Spain  

  166. 新教室『分子生命環境プロセス』の創始とベンチャー起業:バイオコントロールとバイオインターフェースの二大テーマを掲げて Invited

    堀 克敏

    2017年バイオ工学シンポジウム 

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    Event date: 2017.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  167. バクテリオナノファイバーの発見・解析と被毛微生物の創生 Invited

    堀 克敏

    最新バイオインターフェース研究会 

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    Event date: 2017.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:津   Country:Japan  

  168. AtaA, a new trimeric autotransporter adhesin mediating strong and nonspecific adhesion of bacterial cells Invited

    K. Hori

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    Event date: 2017.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  169. 自己凝集性細菌Acinetobacter sp. Tol 5の異種微生物相互作用の解析

    岩崎啓太、石川聖人、中谷 肇、堀 克敏

    化学工学会第82回年会 

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    Event date: 2017.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京   Country:Japan  

  170. Innovation of bioprocess through the bacterionanofiber AtaA Invited International conference

    K. Hori

    2nd Korea-Japan Smart Biodesign Workshop: Technology exchange for green biotechnology 

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    Event date: 2017.2

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  171. 油脂分解微生物を使った革新的排水処理技術 Invited International conference

    堀 克敏

    中部バイオテクノロジーシンポジウム 

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    Event date: 2017.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  172. Innovation of bioprocess through the bacterionanofiber AtaA Invited International conference

    K. Hori

    i-BioP2016 

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    Event date: 2016.12

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Busan   Country:Korea, Republic of  

  173. Real-time monitoring an intracellular redox state of a methane oxidizing bacterium,Methylococcus capsulatus International conference

    M.Ishikawa,Y.Tanaka,S.Kato,S.Nakanishi,K.Hori

    i-BioP2016 

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    Event date: 2016.12

    Language:English   Presentation type:Poster presentation  

    Country:Korea, Republic of  

  174. 驚異の接着性バクテリオナノファイバーAtaAの特性と応用展望 Invited

    堀 克敏

    R&D懇話会 第190定例会 

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    Event date: 2016.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  175. 微生物細胞やバクテリオナノファイバーの表面化学

    堀 克敏

    真空・表面科学合同講演会 

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    Event date: 2016.12

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  176. TpgA, a new peptidoglycan-binding protein forming a complex of the unique acinetobacter trimeric autotransporter adhesin AtaA International conference

    K. Hori, S. Yoshimoto, M. Ishikawa

    1st Zing Protein Secretion in Bacteria Conference 

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    Event date: 2016.11

    Language:English   Presentation type:Poster presentation  

    Venue:Tampa   Country:United States  

  177. Molecular insight into a periplasmic protein interacting with trimeric autotransporter adhesin and peptidoglycan International conference

    S. Yoshimoto, M. Ishikawa, J. Kanie, R. Hiroshige

    1st Zing Protein Secretion in Bacteria Conference 

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    Event date: 2016.11

    Language:English   Presentation type:Poster presentation  

    Venue:Tampa   Country:United States  

  178. The contribution of translocation domain in the assembly of trimeric autotransporter adhesins International conference

    J. Kanie, Y. Ohara, H. Nakatani, K. Hori

    1st Zing Protein Secretion in Bacteria Conference 

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    Event date: 2016.11

    Language:English   Presentation type:Poster presentation  

    Venue:Tampa   Country:United States  

  179. 接着性ナノファイバーたんぱく質AtaAの分泌を促進するたんぱく質TpgAの発見

    吉本将悟、石川聖人、廣重竜太、鈴木淳巨、堀 克敏

    第68回日本生物工学会大会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:富山国際会議場   Country:Japan  

  180. グラム陰性菌由来のファイバーたんぱく質による微生物の固定化と表層提示技術の統合

    中谷肇、小原優季、堀 克敏

    第68回日本生物工学会大会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:富山国際会議場   Country:Japan  

  181. メタン酸化細菌の細胞内レドックス状態の可視化

    石川聖人、田中裕也、田中謙也、木村恒太、加藤創一郎、中西周次、堀 克敏

    第68回日本生物工学会大会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:富山国際会議場   Country:Japan  

  182. 部分欠損体を用いたナノファイバー蛋白質AtaAの接着メカニズムの探索

    青木 壮太・吉本 将悟・石川 聖人・堀 克敏

    日本化学会 第10回バイオ関連化学シンポジウム 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:石川県立音楽堂 もてなしドーム地下イベント広場   Country:Japan  

  183. 接着性ナノファイバータンパク質AtaA分子の接着特性解析

    石井慧・吉本将悟・堀 克敏

    日本化学会 第10回バイオ関連化学シンポジウム 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:石川県立音楽堂 もてなしドーム地下イベント広場   Country:Japan  

  184. バイオコントロールによる高濃度油脂含有排水のハイパフォーマンス処理

    堀 克敏、藤岡正剛

    化学工学会 第48回秋季大会 

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    Event date: 2016.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:徳島大学 常三島キャンパス   Country:Japan  

  185. バクテリオナノファイバーAtaAによるバイオプロセスの革新 Invited

    堀 克敏

    バイオマスイノベーション研究会 

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    Event date: 2016.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  186. Casamino acids inhibits cell adhesion and autoagglutinotion mediated by a trimeric autotransporter adhesin in an Acinetobacter strain International conference

    Y. Ohara, Y.matsuda, Y.Abe, S.Yoshimoto, K. Hori

    ICAR2016 

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    Event date: 2016.6 - 2016.7

    Language:English   Presentation type:Poster presentation  

    Venue:Malaga   Country:Spain  

  187. Functional and structural characteristics of an Acinetobacter trimeric autotransporter adhesin for bacterial cell adhesion International conference

    K. Hori, K.Koiwai, S.Yoshimoto, D.Linke, M.D.Hartmann, A.N.Lupas, H.Nakatani

    ICAR2016 

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    Event date: 2016.6 - 2016.7

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Malaga   Country:Spain  

  188. Pursuing adhesion mechanism of the AtaA belonging to the TAA family by using in-frame deletion International conference

    S. Aoki, S.Yoshimoto, M.Ishikawa, K. Hori

    ICAR2016 

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    Event date: 2016.6 - 2016.7

    Language:English   Presentation type:Poster presentation  

    Venue:Malaga   Country:Spain  

  189. Creation of lanthanoid binding protein based on natural coiledcoil protein

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    Event date: 2016.3

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  190. Adhesion property of the nanofiber protein AtaA from the highly adhesive bacterium

    吉本将悟、中谷肇、岩崎啓太、堀 克敏

    日本化学会 第96春季年会 

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    Event date: 2016.3

    Language:English   Presentation type:Poster presentation  

    Venue:同志社   Country:Japan  

  191. Adhesion property of the nanofiber protein AtaA from the highly adhesive bacterium

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    Event date: 2016.3

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  192. AtaAを介して付着した微生物の流体中での剥離過程の解析

    石井沙季、古市吉秀、堀 克敏

    化学工学会 第81年会 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:関西大学 千里山キャンパス   Country:Japan  

  193. 油脂分解微生物を利用する低コスト・ハイパフォーマンス排水処理システム

    堀 克敏、藤岡正剛

    化学工学会 第81年会 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:関西大学 千里山キャンパス   Country:Japan  

  194. アシネトバクター属由来の表層ナノファイバーを用いたペプチド提示技術の開発

    中谷肇、堀 克敏

    化学工学会 第81年会 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:関西大学 千里山キャンパス   Country:Japan  

  195. 接着性バクテリオナノファイバーAtaAのグラム陰性細菌への導入とその応用

    小原優季、松田祐毅、中谷肇、堀 克敏

    化学工学会 第81年会 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:関西大学 千里山キャンパス   Country:Japan  

  196. 機能性ナノファイバーで覆われた高付着性微生物細胞の創出

    蟹江純一、小原 優季、中谷肇、堀 克敏

    化学工学会 第81年会 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:関西大学 千里山キャンパス   Country:Japan  

  197. 高接着性タンパク質を利用した微生物固定化技術の効率化

    青木壮太、吉本将悟、平野春香、石川聖人、堀 克敏

    化学工学会 第81年会 

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    Event date: 2016.3

    Language:Japanese   Presentation type:Poster presentation  

    Venue:関西大学 千里山キャンパス   Country:Japan  

  198. The low cost and high performance wastewater treatment system using oil-degrading microorganism International conference

    Ayano, M. Fujioka, K. Hori

    Second International Symposium on Advanced Water Science and Technology 

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    Event date: 2016.2

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  199. Application of a fibrous trimeric autotransporter adhesin from Acinetobacter sp. Tol 5 to the nanofiber display system International conference

    H. Nakatani, K.Hori

    The 6th iBioK Asian Workshop 

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    Event date: 2015.12

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  200. 接着性ナノファイバータンパク質AtaAを介した微生物付着の阻害物質についての検討

    松田 祐毅、吉本将悟、小原 優季、古市 吉秀、堀 克敏

    第67回 日本生物工学会大会 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Poster presentation  

    Venue:鹿児島   Country:Japan  

  201. 接着性ナノファイバータンパク質AtaAと共発現するタンパク質との相互作用

    林 亜友美、吉本将悟、石川聖人、堀 克敏

    第67回 日本生物工学会大会 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Poster presentation  

    Venue:鹿児島   Country:Japan  

  202. バクテリオナノファイバータンパク質AtaAを用いた微生物固定化の有用性の実証

    小原 優季、石川聖人、中谷肇、堀 克敏

    第67回 日本生物工学会大会 

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    Event date: 2015.10

    Language:Japanese   Presentation type:Poster presentation  

    Venue:鹿児島   Country:Japan  

  203. A new and highly effective immobilization method using the bacterionanofiber protein AtaA for microbial cell factory International conference

    K.Hori

    2015 International Workshop on Advanced Industrial Biotechnology and Bioengineering for Sustainable Bioindustry Final Program, Hohhot, Inner Mongolia Autonomous Region of China 

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    Event date: 2015.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Uiles Hotel, Hohhot, Inner Mongolia Autonomous Region of China   Country:China  

  204. Microscopic analysis of the process of bacterial cell adhesion and autoagglutination mediated by the adhesive nanofiber protein ATaA International conference

    K. Hori, S. Yoshimoto, Y. Furuichi

    FEMS 6th Congress of European Microbiologists 

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    Event date: 2015.6

    Language:English   Presentation type:Poster presentation  

    Venue:Maastricht   Country:Netherlands  

  205. Biophysical characterization of a trimeric autotransporter adhesion, ATaA, from the highly adhesive bacterium Acinetobacter sp.Tol5 International conference

    S.Yoshimoto, H.Nakatani, K.Hori

    FEMS 6th Congress of European Microbiologists 

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    Event date: 2015.6

    Language:English   Presentation type:Poster presentation  

    Venue:Maastricht   Country:Netherlands  

  206. Flow cell analysis of the process of bacterial cell adhesion mediated by the adhesive nanofiber protein ATaA under shear stress International conference

    Y. Furuichi, K. Izumitani, K. Hori

    FEMS 6th Congress of European Microbiologists 

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    Event date: 2015.6

    Language:English   Presentation type:Poster presentation  

    Venue:Maastricht   Country:Netherlands  

  207. Characterization of AtaA, an Acinetobacter TAA, immobilizing bacterial cells firmly to various surfaces International conference

    K.Hori

    4th International Symposium of the SFB 766 

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    Event date: 2015.5

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Alte Aula, Tubingen   Country:Germany  

  208. 高付着性細菌由来ナノファイバータンパク質AtaAの分離精製と接着特性解析

    吉本将悟、中谷肇、堀 克敏

    日本化学会 第95春季年会 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:日本大学   Country:Japan  

  209. 高接着性タンパク質AtaAのパッセンジャードメインC末端領域の機能・構造解析 International conference

    平野春香、吉本将悟、小祝孝太郎、NORSHARIFFUDIN Nur`izzah、三木章弘、LUPAS Andrei、LINKE Dirk,HARTMANN Marcus、鈴木淳巨、堀 克敏

    日本大学 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  210. AtaAによる凝集・付着特性の顕微解析Ⅱ: せん断応力の観点から

    泉谷啓太、古市吉秀、堀 克敏

    化学工学会 第80年会(春) 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:芝浦工業大学   Country:Japan  

  211. 多機能性ナノファイバーで覆われた被毛微生物細胞の創出

    蟹江純一、中谷肇、堀 克敏

    化学工学会 第80年会(春) 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  212. AtaAによる凝集・付着特性の顕微解析I: コロイド化学の観点から

    古市吉秀、吉本将悟、堀 克敏

    化学工学会 第80年会(春) 

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    Event date: 2015.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  213. Bacteronanofiber protein AtaA useful for immobilization of microbial cell factory International conference

    Katsutoshi Hori

    2014 Asia biohylinks (ABHL ) meeting : Asia biohydrogen and biorefinery (ABB) symposium 

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    Event date: 2014.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Melaka   Country:Malaysia  

  214. Utilizing GCN4-leucine-zipper tag in folding problem of AtaA

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    Event date: 2014.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  215. The time profile of cell adhesion of the highly adhesive bacterium Acinetobacter sp. Tol5 International conference

    Y. Furuichi, K. Izumitani, S. Yoshimoto, K. Hori

    ICAR2014 

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    Event date: 2014.10

    Language:English   Presentation type:Poster presentation  

    Venue:Madrid   Country:Spain  

  216. Characterization of a trimeric autotransporter adhesin from a highly adhesive bacterium Acinetobacter sp. Tol5 International conference

    S. Yoshimoto, H. Nakatani, K. Hori

    ICAR2014 

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    Event date: 2014.10

    Language:English   Presentation type:Poster presentation  

    Venue:Madrid   Country:Spain  

  217. Adhesion property of the highly adhesive bacterium acineotbacter sp. Tol5 mediated by a new trimeric authotransporter adhesin International conference

    K. Hori, Y. Miyachi, H. Nakatani, S. Yoshimoto, Y. Furuichi, M. Ishikawa

    ICAR2014 

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    Event date: 2014.10

    Language:English   Presentation type:Poster presentation  

    Venue:Madrid   Country:Spain  

  218. ナノファイバー蛋白質AtaAによる微生物接着の制御

    吉本将悟、中谷肇、堀 克敏

    第66回 日本生物工学会大会 

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    Event date: 2014.9

    Language:English   Presentation type:Oral presentation (general)  

    Venue:札幌コンベンションセンター   Country:Japan  

  219. バクテリオナノファイバーAtaAのポストアッセンブリー修飾

    永谷和子、中谷肇、堀 克敏

    第66回 日本生物工学会大会 

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    Event date: 2014.9

    Language:English   Presentation type:Oral presentation (general)  

    Venue:札幌コンベンションセンター   Country:Japan  

  220. A novel bioprocess using a bacterionanofiber applicable to biogas production International conference

    China-Japan Forum on Advanced C0/C1 Biotechnology 

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    Event date: 2014.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:China  

  221. 高付着性ナノファイバータンパク質AtaAのアシネトバクター属TAA保存領域の結晶構造解析 International conference

    小祝 孝太郎,Izzah Nur,堀 克敏

    ;第14回日本蛋白質科学会 

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    Event date: 2014.6

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜   Country:Japan  

  222. Structural analysis of Acinetobacter species-conserved domains of a highly adhesive bacterionanofiber protein, AtaA

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    Event date: 2014.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  223. 蛍光ナノファイバーで覆われた被毛微生物細胞の創出

    蟹江純一,中谷肇,堀克敏

    日本化学会第94春季大会(2014) 

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    Event date: 2014.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  224. 水晶発振子を用いた高付着性最近由来ナノファイバータンパク質AtaAの接着特性解析

    吉本将悟,中谷肇,Norshariffudin Nur`Izzah,小祝孝太郎,鈴木淳巨,堀 克敏

    日本化学会第94春季大会(2014) 

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    Event date: 2014.3

    Language:English   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  225. Analyzing the bacterial adhesion of the highly adhesive bacterium Acinetobacter sp.Tol5 using atomic force microscopy

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    Event date: 2014.3

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  226. 蛍光ナノファイバーで覆われた被毛微生物細胞の創出

    蟹江純一,中谷肇,堀克敏

    化学工学会第79年会 

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    Event date: 2014.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岐阜   Country:Japan  

  227. 新規バクテリオナノファイバー蛋白質AtaAの構造・機能と応用技術

    堀克敏,石川聖人,中谷肇,吉本将悟,小原優季,蟹江純一

    化学工学会第79年会 

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    Event date: 2014.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岐阜   Country:Japan  

  228. 機能性周毛を持つ細菌の創出

    中谷肇,蟹江純一,堀 克敏

    化学工学会第79年会 

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    Event date: 2014.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岐阜   Country:Japan  

  229. ナノファイバー蛋白質AtaAの接着特性に基づく微生物の可逆的固定化法

    吉本将悟,中谷肇,堀克敏

    化学工学会第79年会 

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    Event date: 2014.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岐阜   Country:Japan  

  230. 細菌固定化蛋白質の構造・機能と応用

    堀克敏

    CFC&SBJデザイナブルバイオインターフェイスワークショップ 

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    Event date: 2014.2

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:福岡   Country:Japan  

  231. A nanofibrous immobilizing protein for interfacial microbial engineering International conference

    Katsutoshi Hori

    Korea-Japan Smart Biodesign Workshop Technology exchange for green biotechnology 

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    Event date: 2014.1

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  232. 上向流式リアクタで馴養した嫌気性アンモニア酸化細菌グラニュールの構造解析

    黒住明大、渡邊哲文、福崎康博、中村安宏、川久保祐貴、堀克敏

    第29回日本微生物生態学会大会 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島   Country:Japan  

  233. Acinetobacter属用レポーター遺伝子システムの構築とTol 5株の接着遺伝子ataAのプロモーター活性測定

    寺田瑛美、中谷肇、石川聖人、堀 克敏

    第29回日本微生物生態学会大会 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島   Country:Japan  

  234. 高付着性Acinetobacter属細菌Tol 5株の付着とバイオフィルム形成を阻害する新規バクテリオナノファイバーFilピリ

    石川智也、石川聖人、堀克敏

    第29回日本微生物生態学会大会 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島   Country:Japan  

  235. 高付着性細菌Acinetobacter sp.Tol 5株のfim遺伝子群の発現解析

    岡野葵、石川聖人、中谷肇、堀克敏

    第29回日本微生物生態学会大会 

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    Event date: 2013.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島   Country:Japan  

  236. Bacterionanofiber mediating cell adhesion and its application International conference

    Katsutoshi Hori

    Asia Bio-HyLinks2013 

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    Event date: 2013.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  237. Immobilization of Enterobacter aerogenes by a trimetric autotransporter adhesin, AtaA and its application to bio-hydrogen production International conference

    N. Ding, K. Shigemori, H. Nakatani, K. Hori

    Asia Bio-HyLinks 2013 

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    Event date: 2013.11

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  238. Construction of novel bacterial immobilization model using high adhesive nanofiber protein AtaA, International conference

    Y. Ohara, M. Ishikawa, H. Nakatani, K. Hori

    Asia Bio-HyLinks 2013 

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    Event date: 2013.11

    Language:English   Presentation type:Poster presentation  

    Country:Japan  

  239. 高付着性細菌Acinetobacter sp.Tol 5株の新規ナノファイバーFilピリの機能解析

    石川智也,石川聖人,堀克敏

    第7回バイオ関連化学シンポジウム 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  240. 微生物由来ナノファイバータンパク質の接着特性解析 International conference

    吉本将悟、中谷肇、堀 克敏

    第7回バイオ関連化学シンポジウム 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋   Country:Japan  

  241. 高付着性細菌Acinetobacter sp.Tol 5株のfim遺伝子群の機能解析

    岡野葵、石川聖人、中谷肇、堀 克敏

    第7回バイオ関連化学シンポジウム 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋   Country:Japan  

  242. 接着性ナノファイバー蛋白質AtaAによる微生物固定化モデルの構築及び応用 International conference

    小原優季、石川聖人、中谷肇、堀 克敏

    第7回バイオ関連化学シンポジウム 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:名古屋   Country:Japan  

  243. 高付着性タンパク質AtaAの膜アンカードメイン置換体の機能評価

    永井美帆、石川聖人、中谷肇、堀 克敏

    第65回日本生物工学会大会 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:広島   Country:Japan  

  244. 接着性ナノファイバー蛋白質AtaAによる新規微生物固定化法の有効性:Acinetobacter sp.ADP1株によるエステラーゼ反応をモデルに

    小原優季、石川聖人、中谷肇、堀 克敏

    第65回日本生物工学会大会 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:広島   Country:Japan  

  245. バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

    堀克敏

    化学工学会第45回秋季大会 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:岡山   Country:Japan  

  246. 接着性細胞表層タンパク質の特性に基づく微生物固定化技術

    吉本将悟、中谷肇、堀 克敏

    化学工学会第45回秋季大会 

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    Event date: 2013.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:岡山   Country:Japan  

  247. AtaA, a new autotransporter adhesin mediating nonspecific, strong adhesion International conference

    K. Hori

    IGER International Symposium on Cell Surface Structures and Functions 

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    Event date: 2013.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  248. A new trimeric autotransporter adhesin from Acinetobacter sp. Tol 5 mediating high adhesiveness to various abiotic surfaces International conference

    M. Ishikawa, H. Nakatani, K. Hori

    FEMS 5th Congress of European Microbiologists 

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    Event date: 2013.7

    Language:English   Presentation type:Poster presentation  

    Venue:Leipzig   Country:Germany  

  249. AtaA, a new member of the trimeric autotransporter adhesins from Acinetobacter sp. Tol 5 mediating high adhesiveness to various abiotic surfaces International conference

    M.Ishikawa, H.Nakatani,K. Koiwai, K.Hori

    3rd International Symposium of the SFB 766 

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    Event date: 2013.5

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Kaufbeuren   Country:Germany  

  250. A new nanofiber protein which immobilizes gram-negative bacteria onto various material surfaces International conference

    M. Ishikawa, H. Nakatani, K. Shigemori, S. Yoshimoto, A. Suzuki, K. Hori

    German-Japanese International Workshop for Structure and Control for Interfaces 

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    Event date: 2013.1

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Berlin   Country:Germany  

  251. 水中における微生物の付着・バイオフィルム形成と水処理への応用

    堀 克敏

    水の先進理工学第183委員会 

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    Event date: 2012.11 - 2012.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋   Country:Japan  

  252. Wastewater treatment using biofilms International conference

    K.Hori

    ISAWST-1 

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    Event date: 2012.11

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  253. Bacterionanofiber mediating cell adhesion and its application International conference

    K. Hori

    ISABE2012 

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    Event date: 2012.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:China  

  254. Adhesion profiles of Acinetobacter sp.Tol 5 mediated by nanofiber International conference

    S.Yoshimoto,M.Ishikawa,K.Shigemori,H.Nakatani,A.Suzuki,K.Hori

    ISABE2012 

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    Event date: 2012.10

    Language:English   Presentation type:Oral presentation (general)  

    Country:China  

  255. バクテリオナノファイバーの解析と界面微生物工学への展開

    堀 克敏

    第64回日本生物工学会大会 

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    Event date: 2012.10

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:神戸   Country:Japan  

  256. 二つの自殺ベクターを用いた長鎖遺伝子断片の切除

    石川聖人,堀 克敏

    第64回日本生物工学会大会 

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    Event date: 2012.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸   Country:Japan  

  257. バクテリオナノファイバーAtaAを応用した固定化微生物細胞によるインディゴ生産

    重盛一希、石川聖人、堀 克敏

    第64回日本生物工学会大会 

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    Event date: 2012.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸   Country:Japan  

  258. バクテリオナノファイバーを介したペプチド表層提示技術

    中谷 肇, 石川 聖人, 堀 克敏

    第11回微生物研究会 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京   Country:Japan  

  259. ナノファイバーを介したAcinetobacter sp. Tol 5

    吉本 将悟, 重盛 一希, 石川 聖人, 堀 克敏

    第11回微生物研究会 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Poster presentation  

    Venue:東京   Country:Japan  

  260. 界面微生物工学の廃水処理における応用成功事例

    堀 克敏

    第28回日本微生物生態学会大会 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:豊橋   Country:Japan  

  261. バクテリオナノファイバーが繋ぐもの

    堀 克敏

    分子ロボティクス研究会 

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    Event date: 2012.4

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋   Country:Japan  

  262. Acinetobacter sp.Tol 5の非生物表面への付着の評価

    重盛 一希、石川 聖人、吉本 将悟、堀 克敏

    化学工学会第77年会 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:桃居   Country:Japan  

  263. バイオファウリングの原因解明と抑制技術

    堀 克敏

    膜機構春季講演会 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:神戸   Country:Japan  

  264. Bacterionanofibers applicable to a new method for surface immobilization of microbial cells International conference

    K.Hori

    14th Asia Pacific Confederation of Chemical Engineering Congress 

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    Event date: 2012.2

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Singapore  

  265. 日中バイオ水素研究の日本側研究概要

    堀 克敏

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    Event date: 2012.2

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:大阪   Country:Japan  

  266. 身近な微生物の驚くべき能力とその利用を目指す微生物研究の最前線

    堀 克敏

    さかえサイエンストーク 

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    Event date: 2011.10

    Language:Japanese  

    Venue:名古屋   Country:Japan  

  267. Bacterionanofiber responsible for cell adhesion and its application International conference

    K. Hori, M. Ishikawa

    17th Symposium of Young Asian Biochemical Engineers' community 

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    Event date: 2011.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:Incheon   Country:Korea, Republic of  

  268. 接着バクテリオナノファイバーのバイオプロセスへの応用

    石川 聖人、重盛 一希、堀 克敏

    第43回化学工学会秋季大会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  269. バクテリオナノファイバー蛋白質と界面微生物工学への展開

    堀 克敏

    第5回バイオ関連化学シンポジウム 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:つくば   Country:Japan  

  270. A unique nanofibrous adhesion confers nonspecific, high adhesiveness to bacteria International conference

    K.Hori

    Interfacial Microbial Engineering, International Union of Microbiological Societies 2011 

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    Event date: 2011.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  271. バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

    堀 克敏

    テクノ・フェア2011 名大もの作り最前線-創造から技術へ- 

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    Event date: 2011.9

    Language:Japanese  

    Venue:名古屋   Country:Japan  

  272. 界面微生物工学によるグリーンイノベーションを目指して

    堀 克敏

    微生物とコロイドのソフト界面 

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    Event date: 2011.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:つくば   Country:Japan  

  273. Microbial degradation of biofoulants in a membrane bioreactor for water purification International conference

    D. Okamura, Y. Mori, T. Hashimoto, K. Hori

    Defense Science & Research Conference 

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    Event date: 2011.8

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Singapore  

  274. Bacterial cell Adhesion through bacterionanofibers International conference

    K.Hori

    BAB2011 -25th Bacterial Adherence and Biofilm 

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    Event date: 2011.7

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:The Jikei University School of Medicine   Country:Japan  

  275. A new bacterionanofiber protein and its application to cell immobilization International conference

    K. Hori

    The 1st iBioK Asian Symposium 

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    Event date: 2010.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  276. Production of peritrichate bacterionanofibers and their proteinaceous components by Acinetobacter sp. Tol 5 cells International conference

    K. Hori, M. Ishikawa, M. Yamada, Y. Ishikawa, H. Ebi

    World Network Seminar on Advanced Particle Science and Technology 

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    Event date: 2010.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  277. バクテリオナノファイバー蛋白質の分子解析と界面微生物工学への展開

    堀 克敏

    第62回日本生物工学会要旨集,P. 232 

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    Event date: 2010.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎   Country:Japan  

  278. 高付着性Acinetobacter ap. Tol 5の三量体型オートトランスポーターアドヘシン遺伝子下流にコードされる外膜リポ蛋白質OmlTの分子解析

    久野雅大、石川聖人、石川 裕、堀 克敏

    第62回日本生物工学会要旨集,P. 14 

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    Event date: 2010.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎   Country:Japan  

  279. Acinetobacter sp. ADP1の三量体オートトランスポーターアドヘシン領域の分子解析

    石川 裕、石川聖人、堀 克敏

    第62回日本生物工学会要旨集,P. 13 

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    Event date: 2010.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:宮崎   Country:Japan  

  280. 微生物直接固定化の新技術:微生物に非特異的付着性及び凝集性を付与する遺伝子 International conference

    堀 克敏

    第62回日本生物工学会要旨集,P. 221 

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    Event date: 2010.10

    Language:English   Presentation type:Oral presentation (general)  

    Venue:宮崎   Country:Japan  

  281. 細菌の非特異的付着をもたらす接着ナノファイバー International conference

    石川聖人、堀 克敏

    粉体工学会46回夏季シンポジウム「ナノ粒子・人工材料と相互作用する生物」 

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    Event date: 2010.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:京都   Country:Japan  

  282. バクテリオナノファイバーによる非特異的付着と工学利用 International conference

    堀 克敏

    生物工学会第1回中部支部例会 

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    Event date: 2010.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋   Country:Japan  

  283. A new bacterionanofibers protein and its application to cell immobilization International conference

    K. Hori

    2010 International Symposium on Advanced Biological Engineering and Science 

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    Event date: 2010.7

    Language:English   Presentation type:Oral presentation (keynote)  

    Country:Japan  

  284. 油水界面単層吸着微生物の高速物質変換への活用 International conference

    堀 克敏

    有機溶媒耐性微生物利用技術研究会シンポジウム「疎水性の世界でのバイオプロダクションを目指して」 

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    Event date: 2010.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東京   Country:Japan  

  285. 材料表面と微生物細胞の相互作用と付着機構 International conference

    堀 克敏

    金属材料への生物付着と相互作用研究会 

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    Event date: 2010.1

    Language:Japanese   Presentation type:Oral presentation (keynote)  

    Venue:鈴鹿   Country:Japan  

  286. Langmuir adsorption of hydrophobic bacterial cells to the oil surface for highly efficient conversion of a hydrophobic and toxic substrate International conference

    K. Hori

    APBioCheEC2009 

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    Event date: 2009.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  287. Molecular analysis of cell surface components responsible for cell hydrophobicity of Pseudomonas sp. TIS1-127 International conference

    H. Ebi, N. Hiramatsu, M. Nannbu, K. Kanie, M. Okochi, H. Honda, K. Hori

    APBioCheEC2009 

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    Event date: 2009.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  288. Effect of a carbon source on the adhesive property and cell surface morphology of the highly adhesive bacterium Acinetobacter sp. Tol 5 International conference

    Y. Ishikawa, H. Ebi, A. Higuchi, M. Ishikawa, K. Hori

    APBioCheEC2009 

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    Event date: 2009.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  289. Identification of adhesive protein composing a bacterial nanofiber on a highly sticky bacterium, Acinetobacter sp. Tol 5 International conference

    M. Ishikawa, S. Takada, A. Higuchi, Y. Hotta, K. Yamamoto, K. Hori

    APBioCheEC2009 

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    Event date: 2009.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  290. Symbiotic effect of a lipase-secreting bacterium and a glycerol-assimilating yeast on triacylglycerol degradation applicable to construction of a functional biofilm International conference

    K. Hori, A. Miura, X. Li, H. Matsuoka, S. Matsumoto

    5th conference of Biofilms 

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    Event date: 2009.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Mexico  

  291. Identification of biofoulant of membrane bioreactors in soluble microbial products International conference

    D. Okamura, Y. Mori, T. Hashimoto, K. Hori

    5th conference of Biofilms 

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    Event date: 2009.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Mexico  

  292. Identification of a novel autotransporter adhesin contributing to non-specific adhesion in a highly adhesive bacterium International conference

    M. Ishikawa, S. Takada, A. Higuchi, K. Hori

    Acinetobacter sp. Tol5 

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    Event date: 2009.11

    Language:English   Presentation type:Oral presentation (general)  

    Country:Mexico  

  293. 粘着性細菌由来のタンパク質性ナノファイバーの分析と応用 International conference

    堀 克敏

    酵素工学研究会第62回講演会 

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    Event date: 2009.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:東京   Country:Japan  

  294. 細菌ナノファイバーによる微生物の表面付着 International conference

    堀 克敏

    第61回日本生物工学会大会 

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    Event date: 2009.9

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋   Country:Japan  

  295. 芳香族水酸化細菌Pseudomonas sp.TITS-127の疎水性に関わる細胞表層の分子解析

    海老 博則、平松 直人、南部 真理、蟹江 慧、大河内 美奈、本多 裕之、堀 克敏

    第61回日本生物工学会大会要旨集,P.172 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  296. 炭素源がAcinetobactor sp.Tol5の付着性と細胞表層形態へ及ぼす影響

    石川 裕、海老 博則、海老 博則、石川 聖人、堀 克敏

    第61回日本生物工学会大会要旨集P.172 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  297. 高付着性細菌Acinetobactor sp.Tol5のオートトランスポーターアドへシンの機能解析

    石川 聖人、海老 博則、石川 裕、堀 克敏

    第61回日本生物工学会大会要旨集,P.10 

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    Event date: 2009.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  298. Monolayer adsorption of hydrophobic bacterial cells to the oil surface for highly efficient chemical-conversion at the oil-water interfaces International conference

    K. Hori

    14th European Congress on Biotechnology 

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    Event date: 2009.9

    Language:English   Presentation type:Oral presentation (general)  

    Country:Spain  

  299. Bacterial nanofibers applicable to immobilization and surface display of industrial microorganisms International conference

    K. Hori

    BioEco 2009 

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    Event date: 2009.6

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:China  

  300. Adhesive nanofibers for bacterial adhesion International conference

    K. Hori

    7th Seminar of advanced particle handling science 

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    Event date: 2009.4

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  301. Adhesive nanofibers for bacterial adhesion International conference

    K. Hori

    BIT's 2ndAnnual World Congress of Industrial biotechnology 2009 

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    Event date: 2009.4

    Language:English   Presentation type:Oral presentation (keynote)  

    Country:Korea, Republic of  

  302. バイオフィルム形成に関わる細菌ナノファイバー International conference

    堀 克敏

    日本農芸化学会 

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    Event date: 2009.3

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:札幌   Country:Japan  

  303. 界面微生物工学とは International conference

    堀 克敏

    日本紛体工業技術協会第31回技術情報交流懇話会 

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    Event date: 2009.2

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋   Country:Japan  

  304. 細菌粘着性ナノファイバーによる粘着メカニズムと工学利用 International conference

    堀 克敏

    粘着研究会第107回例会 

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    Event date: 2009.1

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:大阪   Country:Japan  

  305. トリアシルグリセロール分解におけるリパーゼ分泌細菌Burkholderia arboris SL1B1およびグリセロール資化酵母Candida cylindracea SL1B2の共生効果

    松岡浩史、三浦篤人、堀 克敏

    農芸化学会2009年度大会講演要旨集, P. 327 

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    Event date: 2009

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  306. Acinetobacter sp. Tol 5の粘着性ナノファイバータイプ1ピリの解析2:組換え蛋白質精製と発現解析

    山田 真理子、石川 聖人、樋口 愛介、堀 克敏

    化学工学会第74年会要旨集, S302 

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    Event date: 2009

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜   Country:Japan  

  307. Acinetobacter sp. Tol 5の粘着性ナノファイバータイプ1ピリの解析1:遺伝子解析 International conference

    石川紘子、井坂仁美、石川 聖人、堀 克敏

    化学工学会第74年会要旨集, S301 

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    Event date: 2009

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜   Country:Japan  

  308. 油脂分解微生物の混合培養によるトリアシルグリセロールの効率的分解

    松岡浩史、三浦篤人、堀 克敏

    化学工学会第74年会要旨集, R309 

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    Event date: 2009

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜   Country:Japan  

  309. Bacterial nanofibers for cell adhesion International conference

    K. Hori

    17th Nisshin Engineering Particle Technology International Seminar 

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    Event date: 2008.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  310. 微生物の粘着機構と工学利用 International conference

    堀 克敏

    第7回産官学接着若手フォーラム 

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    Event date: 2008.12

    Language:English   Presentation type:Oral presentation (invited, special)  

    Venue:名古屋   Country:Japan  

  311. バイオフィルム形成に関わる細菌ナノファイバー International conference

    堀 克敏

    第24回日本微生物生態学会 

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    Event date: 2008.11

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

    Venue:札幌   Country:Japan  

  312. Interaction between bacterial cells and Interfaces International conference

    K. Hori

    CRC International Symposium on Bio-interface and Biomass Conversion 

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    Event date: 2008.10

    Language:English   Presentation type:Oral presentation (invited, special)  

    Country:Japan  

  313. Langmuir adsorption of bacterial cells to the oil surface for green technologies International conference

    H. Watanabe, Y. Tanji, H. Unno

    13th International Biotechnology Symposium and Exhibition 

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    Event date: 2008.10

    Language:English   Presentation type:Oral presentation (general)  

    Country:China  

  314. Bacterial nanofibers on Acinetobacter sp. Tol 5 International conference

    A. Higuchi, Y. Hotta, K. Yamamoto, K. Hori

    13th International Biotechnology Symposium and Exhibition 

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    Event date: 2008.10

    Language:English   Presentation type:Oral presentation (general)  

    Country:China  

  315. A novel proteineous adhesin of a highly sticky gram-negative bacterium International conference

    M. Ishikawa, S. Takada, K. Hori

    13th International Biotechnology Symposium and Exhibition 

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    Event date: 2008.10

    Language:English   Presentation type:Oral presentation (general)  

    Country:China  

  316. Molecular analysis of the highly adhesive bacterium, Acinetobacter sp. Tol 5 International conference

    M. Ishikawa, S. Takada, K. Hori

    5th International Conference Interfaces against Pollution 

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    Event date: 2008.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  317. Morphological analysis of bacterial nanofibers on Acinetobacter sp. strain Tol 5 by electron microscopy International conference

    A. Higuchi, Y. Hotta, K. Yamamoto, K. Hori

    5th International Conference Interfaces against Pollution 

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    Event date: 2008.6

    Language:English   Presentation type:Oral presentation (keynote)  

    Country:Japan  

  318. Bacterial adhesion and cell surface structure International conference

    K. Hori

    5th International Conference Interfaces against Pollution 

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    Event date: 2008.6

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  319. 粘着性細菌ナノファイバーのネガティブ染色法の検討

    樋口愛介、堀田康明、山本宏治、堀 克敏

    医学生物学電子顕微鏡技術学会第24回学術講演会予稿集,P36 

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    Event date: 2008.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:厚木   Country:Japan  

  320. The role of bacterial nanofibers for cell adhesion International conference

    K. Hori

    2nd International Symposium on Advanced Biological Engineering and Science 

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    Event date: 2008.4

    Language:English   Presentation type:Oral presentation (keynote)  

    Venue:Beijing   Country:China  

  321. Molecular analysis of a new autotransporter adhesin of highly adhesive bacterium, Acinetobacter sp. Tol5 International conference

    M. Ishikawa, S. Takada, K. Hori

    2nd International Symposium on Advanced Biological Engineering and Science 

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    Event date: 2008

    Language:English   Presentation type:Oral presentation (general)  

    Country:China  

  322. グリーンテクノロジーに有用な芳香環酸化細菌の有機溶媒への細胞単層吸着

    堀 克敏、渡辺寿美、丹治保典、海野 肇

    化学工学会第40回秋季大会要旨集, C306 

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    Event date: 2008

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

  323. 高付着性細菌の粘着因子の分子解析

    石川 聖人、高田 修平、堀 克敏

    化学工学会第73回年会要旨集,J207 

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    Event date: 2008

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:静岡   Country:Japan  

  324. 高付着性グラム陰性細菌Acinetobacter sp. Tol 5のオートトランスポーター粘着蛋白質の分子解析

    石川 聖人、高田 修平、堀 克敏

    農芸化学会2008年度大会講演要旨集,P. 80 

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    Event date: 2008

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  325. 高付着性・疎水性細菌のAcinetobacter sp. Tol 5の“禿げ変異株”細胞の有機溶媒表面への単層吸着

    堀 克敏、渡辺寿美、石井俊一、丹治保典、海野 肇

    農芸化学会2008年度大会講演要旨集,P. 29 

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    Event date: 2008

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  326. 高疎水性トルエン分解細菌の有機溶媒への単層吸着

    堀 克敏、渡辺寿美、丹治保典、海野 肇

    第60回生物工学会 

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    Event date: 2008

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  327. 油表面への細菌細胞の単層吸着と有機溶媒の高速変換

    堀 克敏、渡辺寿美、丹治保典、海野 肇

    第三回バイオ関連化学合同シンポジウム要旨集, P.0291 

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    Event date: 2008

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜   Country:Japan  

  328. 細菌の表層構造と界面付着 International conference

    堀 克敏

    Pre-seminar of 5th International conference Interfaces against Pollution「土・水・生命環境とコロイド界面現象07」 

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    Event date: 2007.8

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:つくば大学   Country:Japan  

  329. Bacterial nanofiber responsible for cell adhesion to solid surfaces International conference

    K. Hori

    HITS Bionanotechnology symposium 2007 

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    Event date: 2007.4

    Language:English   Presentation type:Oral presentation (general)  

    Venue:Ansan   Country:Korea, Republic of  

  330. 微生物及びバイオフィルムの機能と応用 International conference

    堀 克敏

    日本セラミックマシナリー協会 2006年度第2回講演会 

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    Event date: 2007.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  331. 新規細菌粘着蛋白質の分子解析

    石川聖人、高田修平、堀 克敏

    第59回日本生物工学会大会要旨集, P. 129 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:広島   Country:Japan  

  332. 高付着性細菌の細菌ナノファイバーと粘着特性

    堀 克敏、高田修平、石川聖人、秋田圭一、石井俊一、海野 肇

    化学工学会第72年会講演要旨集,H306 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都   Country:Japan  

  333. 強付着性細菌Acinetobacter sp. Tol 5のナノファイバーと粘着蛋白質

    堀 克敏、秋田圭一、高田修平、石川聖人、樋口愛介、藤村幸裕、井坂仁美

    農芸化学会2007年度大会要旨集, P.174 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  334. Pseudomonas putida TIS127の細胞表面疎水性に関わる蛋白質の解析

    福田弘幸、蟹江 慧、大河内美奈、本多裕之、大津厳生、高木博史、堀 克敏

    日本化学会第10回バイオテクノロジー部会シンポジウム要旨集, P.97 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  335. 高付着性細菌Acinetobacter sp. Tol 5株の粘着ファイバータイプ1ピリの遺伝子解析

    井坂仁美、仲西賢剛、堀 克敏

    日本化学会第10回バイオテクノロジー部会シンポジウム要旨集, P.96 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  336. Acinetobacter 属細菌Tol 5株細胞における粘着性ナノファイバーの形成

    樋口愛介、宮田俊介、堀田康明、山本宏治、堀 克敏

    日本化学会第10回バイオテクノロジー部会シンポジウム要旨集, P.95 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  337. 高付着性グラム陰性細菌の粘着ナノファイバーの分子解析

    高田修平、石川聖人、堀 克敏

    日本化学会第10回バイオテクノロジー部会シンポジウム要旨集, P.94 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  338. 超疎水性菌のトランスポゾン変異体作成と疎水性機構の解析

    蟹江慧、大河内美奈、福田弘幸、堀 克敏、本多裕之

    化学工学会第39回秋季大会要旨集, 30F031 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北海道   Country:Japan  

  339. 高付着性細菌Acinetobacter sp. Tol 5の粘着遺伝子の解析

    高田修平、石川聖人、宮田俊介、堀 克敏

    化学工学会第39回秋季大会要旨集, 30F030 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:北海道   Country:Japan  

  340. Acinetobacter 属細菌Tol 5株細胞における粘着性ナノファイバーの形成

    樋口愛介、宮田俊介、堀田康明、山本宏治、堀 克敏

    第59回日本生物工学会大会要旨集, P. 92 

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    Event date: 2007

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:広島   Country:Japan  

  341. 微生物とバイオフィルムの機能と応用 International conference

    堀 克敏

    NPOバイオものづくり中部; 第6回環境分科会 

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    Event date: 2006.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  342. 名古屋工業大学の健康工学プロジェクトについて International conference

    堀 克敏

    あいち健康長寿産業クラスター推進協議会 拠点大学・機関別シーズ発表会シリーズ第1回 名古屋工業大学・健康長寿関連研究シーズ発表会 

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    Event date: 2006.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  343. 細菌の付着機構=感染防止と清浄な環境に向けての新しい取組み International conference

    堀 克敏

    あいち健康長寿産業クラスター推進協議会 拠点大学・機関別シーズ発表会シリーズ第1回 名古屋工業大学・健康長寿関連研究シーズ発表会 

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    Event date: 2006.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  344. 微生物と界面の相互作用と環境技術 International conference

    堀 克敏

    第17回名古屋コンファレンス“実践的環境調和型化学への挑戦” 

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    Event date: 2006.1

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  345. Dynamics in cell adhesion of an extremely adhesive bacterium Acinetobacter sp. Tol 5 to a solid surface International conference

    K. Hori, S. Ishii, H. Unno

    106rd General Meeting of American Society for Microbiology, abstr. J-012 

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    Event date: 2006

    Language:English   Presentation type:Oral presentation (general)  

    Country:United States  

  346. 高付着性細菌の細胞表層構造の解析

    宮田俊介、堀田康明、山本宏治、堀 克敏

    第22回日本微生物生態学会講演要旨集, P.252 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  347. 強付着性細菌Acinetobacter Tol 5の細胞表層構造と固体表面付着機構

    堀 克敏、石井俊一、海野 肇、高田修平、井坂仁美、宮田俊介

    農芸化学会2006年度大会講演要旨集,100 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都   Country:Japan  

  348. 強付着性トルエン分解細菌の固体表面付着機構

    堀 克敏、石井俊一、海野 肇

    化学工学会第71年会講演要旨集,A303 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  349. 細菌のSEM観察のための乾燥法の検討

    宮田俊介、堀 克敏、Rogelio Jose Scougall Vicchis、堀田康明

    医学生物学電子顕微鏡技術学会予稿集,P.49 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岐阜   Country:Japan  

  350. 都市河川堆積ヘドロの曝気浄化過程における化学・生物学的特性の変化

    久米啓介、山崎俊夫、前田健一、堀 克敏

    第41回地盤工学研究会講演要旨集,1164 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:鹿児島   Country:Japan  

  351. Acinetobacter 属細菌Tol 5株の付着遺伝子の解析

    高田修平、石井俊一、海野 肇、堀 克敏

    第58回日本生物工学会大会講演要旨集, P.140 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  352. 親油性バクテリアによる芳香族分解過程の解析

    平松直人、渡辺寿美、福田弘幸、堀 克敏

    第58回日本生物工学会大会講演要旨集, P.151 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  353. 高付着性細菌Acinetobacter sp. Tol 5の持つ新規粘着蛋白質の構造に関する研究

    秋田圭一、堀 克敏

    第22回日本微生物生態学会講演要旨集, P.154 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  354. 新たに単離したトルエン分解細菌の分解酵素遺伝子の解析

    福田弘幸、平松直人、堀 克敏

    第22回日本微生物生態学会講演要旨集, P.134 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  355. 高付着性細菌Acinetobacter sp. Tol 5のadhesin遺伝子の解析 International conference

    高田修平、堀 克敏

    第22回日本微生物生態学会講演要旨集, P.253 

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    Event date: 2006

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  356. 細菌の初期付着機構 International conference

    堀 克敏

    化学工学会セミナー“バイオフィルムの生成機構とその制御” 

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    Event date: 2005.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜   Country:Japan  

  357. トルエンジオキシゲナーゼによるトリクロロエチレンのコメタボリズム International conference

    堀 克敏

    酵素工学研究会第54回講演会講演要旨集, P.9 

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    Event date: 2005.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  358. 細菌に強付着性をもたらす新規細胞表層構造 International conference

    堀 克敏、石井俊一、海野 肇

    医学生物学電子顕微鏡技術学会予稿集,P.20 

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    Event date: 2005.5

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岐阜   Country:Japan  

  359. Restoration of trichloroethylene degradation activity of Pseudomonas putida F1 by the addition of aromatics International conference

    K. Hori, Yuki Morono, J. Mii, Y. Tanji, H. Unno

    The Joint International Symposia for Subsurface Microbiology and Environmental Biogeochemistry (ISSM-ISEB 2005), abstr. 45 

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    Event date: 2005

    Language:English   Presentation type:Oral presentation (general)  

    Country:United States  

  360. メタン発酵共生微生物群が形成する凝集体の観察

    石井俊一、堀 克敏、堀田康明、渡辺一哉

    医学生物学電子顕微鏡技術学会予稿集,P.21 

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    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岐阜   Country:Japan  

  361. トルエン分解菌の細胞表層に存在するアンカー様構造物と固体付着

    堀 克敏、石井俊一、幸喜 順、丹治保典、海野 肇

    化学工学会第70年会講演要旨集, E305 

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    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  362. 細菌のSEM観察のための乾燥法の検討

    宮田俊介、堀 克敏、Rogelio Jose Scougall Vicchis、堀田康明

    医学生物学電子顕微鏡技術学会予稿集,P.49 

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    Event date: 2005

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:岐阜   Country:Japan  

  363. トルエン分解菌の表層細胞にあるアンカー様構造物と固体表面付着機構 International conference

    堀 克敏

    日本微生物生態学会第20回大会 

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    Event date: 2004.11

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

  364. Physiological heterogeneity in biofilm formation ability of a strong adhesive bacterium, Acinetobacte¬r sp. Tol 5 International conference

    S. Ishii, J. Koki, H. Watanabe, Y. Tanji, H. Unno, K. Hori

    Biofilms 2004, proc. 150-155 

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    Event date: 2004

    Language:English   Presentation type:Oral presentation (general)  

    Country:United States  

  365. Influence of iron depletion on growth of cyanobacterium Anabaena circinalis International conference

    B-K. Sun, K. Miyanaga, K. Hori, Y. Tanji, H. Unno

    0th the Asian Pacific Confederation of Chemical Engineering Congress (APCChE), proc. 877:1-6 

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    Event date: 2004

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  366. Alternate aerobic-anaerobic wastewater treatment system for excess sludge reduction International conference

    S. Takano, S-J. Jung, Y. Morono, K. Miyanaga, K. Hori, Y. Tanji, H. Unno

    10th the Asian Pacific Confederation of Chemical Engineering Congress (APCChE), proc. 578:1-7 

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    Event date: 2004

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  367. Tools for display of peptide on the bacteriophage capsid International conference

    Y. Tanji, K. Miyanaga, K. Hori, H. Unno

    10th the Asian Pacific Confederation of Chemical Engineering Congress (APCChE), proc. 77:1-7 

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    Event date: 2004

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  368. Kinetic analysis of trichloroethylene cometabolism by toluene-degrading bacteria International conference

    J. Mii, Y. Morono, Y. Tanji, H. Unno, K. Hori

    10th the Asian Pacific Confederation of Chemical Engineering Congress (APCChE), proc. 610:1-7 

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    Event date: 2004

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  369. Production of polyhudroxyalkanoates from toluene International conference

    M. Abe, H. Unno, K. Hori

    0th the Asian Pacific Confederation of Chemical Engineering Congress (APCChE), proc. 611:1-10 

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    Event date: 2004

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  370. 芳香族基質の添加によるトルエンジオキシナーゼのトリクロロエチレン分解活性の回復

    堀 克敏、諸野祐樹、三井淳一、海野 肇

    第56回日本生物工学会大会講演要旨集 

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    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  371. 芳香族基質の添加によるPseudomonas putida F1のトリクロロエチレン分解能力の回復

    堀 克敏、諸野祐樹、海野 肇

    化学工学会第69年会講演要旨集, O309 

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    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:堺   Country:Japan  

  372. 油水二相系における微生物反応の効率化についての検討

    渡辺寿美・石井俊一・丹治保典・海野 肇・堀 克敏

    第56回日本生物工学会大会講演要旨集,172 

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    Event date: 2004

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  373. Adhesion mechanism of a newly isolated toluene degrading bacterium 1: novel appendages causing tenacious adhesion International conference

    S. Ishii, J. Koki, H. Unno, K. Hori

    11th European Congress on Biotechnology, abstr. 88 

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    Event date: 2003

    Language:English   Presentation type:Oral presentation (general)  

    Country:Switzerland  

  374. Adhesion mechanism of a newly isolated toluene degrading bacterium 2: dynamics of adhesion process International conference

    K. Hori, S. Ishii, J. Koki, Y. Tanji, H. Unno

    11th European Congress on Biotechnology, abstr. 89 

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    Event date: 2003

    Language:English   Presentation type:Oral presentation (general)  

    Country:Switzerland  

  375. Diversity of the substrate range of toluene-degrading bacteria correlated with their growth rates International conference

    K. Hori, Y. Morono, H. Unno

    103rd General Meeting of American Society for Microbiology, abstr. Q-138 

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    Event date: 2003

    Language:English   Presentation type:Oral presentation (general)  

    Country:United States  

  376. The recovery of trichloroethylene degradation ability by the addition of the primary substrate International conference

    Y. Morono, K. Hori, H. Unno

    03rd General Meeting of American Society for Microbiology, abstr. Q-011 

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    Event date: 2003

    Language:English   Presentation type:Oral presentation (general)  

    Country:United States  

  377. Novel structure and properties of the cell surface of Acinetobacter genospecies Tol 5 causing bacterial adhesion to solid surfaces International conference

    S. Ishii, K. Hori, H. Watanabe, Y. Tanji, H. Unno

    The First International Congress on Bio-Nanointerface (ICBN 2003 Tokyo), abstr. 154 

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    Event date: 2003

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  378. トルエン資化菌における基質レンジの多様性とその増殖速度との関連

    諸野祐樹,海野 肇,丹治保典,堀 克敏

    第55回日本生物工学会大会講演要旨集, 195 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:熊本   Country:Japan  

  379. 新規細胞表層構造物による微生物付着機構

    石井俊一,幸喜 順,渡辺寿美,丹治 保典,海野 肇,堀 克敏

    農芸化学会2003年度大会講演要旨集,3A14p20 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  380. 基質添加によるトリクロロエチレン分解能力の回復

    諸野祐樹,海野 肇,丹治保典,堀 克敏

    農芸化学会2003年度大会講演要旨集,2C01a10 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  381. 複数のファージによる大腸菌O157:H7の増殖制御

    島田 拓哉,與市 雅俊,宮永 一彦,堀 克敏,海野 肇,丹治 保典

    化学工学会第36回秋季大会講演要旨集,X1P69 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

  382. トルエン分解菌Acinetobacter genospecies Tol 5の付着メカニズムの解析

    石井 俊一,堀 克敏,幸喜 順,丹治 保典,海野 肇

    化学工学会第36回秋季大会講演要旨集,P3A07 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

  383. 白色腐朽菌が生産するリグニン分解酵素の活性評価とその応用

    宮永 一彦,高 智広,安部 道玄,堀 克敏,丹治 保典,海野 肇

    化学工学会第36回秋季大会講演要旨集,O3P10 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

  384. バチルスファージ溶菌酵素の抗菌作用

    丹治保典,折戸 雄時,宮永 一彦,堀 克敏,海野 肇

    化学工学会第36回秋季大会講演要旨集,O3P09 

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    Event date: 2003

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

  385. Preliminary study on simultaneous production of bioplastic and biosurfactant International conference

    S. Marsudi, K. Hori, H. Unno

    Regional Symposium on Chemical Engineering 2002, proc. 95-101 

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    Event date: 2002

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  386. Isolation, characterization and application of off-Gas treatment of toluence-degrading bacteria International conference

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    Event date: 2002

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  387. Characterization of enzymatic activity and antibacterial activity of endolysin encoded by a Bacillus amyloliquefacience phage International conference

    Y. Tanji, M. Morita, Y. Orito, K. Hori, H. Unno

    XIIth International Congress of Virology, abstr. V-970 

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    Event date: 2002

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  388. Functional analysis of antibacterial activity of Bacillus amyloliquefacience phage endolysisn International conference

    Y. Tanji, M. Morita, Y. Orito, K. Hori, H. Unno

    7th Chaina-Japan Joint Symposium on Enzyme Engineering, proc. 105-106 

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    Event date: 2002

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  389. Pseudomonas putida におけるトリクロロエチレン分解能力の回復

    諸野祐樹,堀 克敏,海野 肇

    日本生物工学会創立80周年記念大会講演要旨集, 150 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  390. ポリヒドロキシアルカン酸とラムノリピッドとの同時生産

    堀 克敏, Sidikmarsudi, 海野 肇

    農芸化学会2002年度大会要旨集, 2-5 Da01 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:仙台   Country:Japan  

  391. 好気-嫌気連続反応を用いた廃水処理システム中の汚泥量評価

    林 山杉,鄭 修貞,宮永一彦,堀 克敏,丹治保典,海野 肇, 早川昌宗,佐野政幸,衛藤俊司

    化学工学会第67年会講演要旨集, T107 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  392. 化学プロセスにおける塩素のフロー解析と塩素化合物の低減

    海野 肇,丹治保典,堀 克敏,宮永一彦

    化学工学会第67年会講演要旨集, Q105 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  393. ポリヒドロシキアルカン酸とラムノリピッドとの同時生産

    堀 克敏,Sidikmarsudi,海野 肇

    化学工学会第67年会講演要旨集, R307 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:福岡   Country:Japan  

  394. 微生物膜に対するバイオサイドの抗菌活性

    丹治保典,中野 肇,堀 克敏,宮永一彦,海野 肇

    材料と環境2002講演要旨集, A-308S 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:川崎   Country:Japan  

  395. ポリヒドロキシアルカン酸とラムノリピッドとの同時生産

    堀 克敏, Sidikmarsudi, 海野 肇

    第51回高分子学会年次大会要旨集,I K11 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜   Country:Japan  

  396. バイオフィルム内菌体に対するバイオサイドの殺菌効果

    丹治保典,中野 肇,宮永一彦,堀 克敏,海野 肇

    化学工学会第35回秋季大会講演要旨集,L203 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸   Country:Japan  

  397. Acinetobacter genospecies Tol 5の担体への付着因子の解析

    石井俊一,堀 克敏,渡辺寿美, 山下茂樹,丹治保典,海野 肇

    化学工学会第35回秋季大会講演要旨集,L213 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸   Country:Japan  

  398. 培養条件がPHAとラムノリピッドの生合成に与える影響

    一戸里江子,堀 克敏, Sidikmarsudi,海野 肇

    化学工学会第35回秋季大会講演要旨集,L302 

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    Event date: 2002

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:神戸   Country:Japan  

  399. A mathematical description for the fungal degradation process of biodegradable plastics International conference

    B.-I. Sang, K. Hori, Y. Tanji, H. Unno

    th International Symposium on Mathematical Modeling and Simulation in Agricultural and Bio-Industries, proc. session F 1-16 

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    Event date: 2001

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  400. Fate of coliphage in waste water treatment process and detection of phages carrying the shiga toxin type 2 gene International conference

    Y. Tanji, K. Mizoguchi, T. Akitsu, M. Morita, K. Hori, H. Unno

    Asian Waterqual 2001, proc I. 685-690 

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    Event date: 2001

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  401. 基質濃度応答型自己破砕バチルスによるPHB生産

    金子真由美, 堀 克敏,X.-H. Xing,一戸里江子

    生物工学会平成13年度大会要旨集,427 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:甲府   Country:Japan  

  402. No title

    I. Susanti, 山本進二郎,宮永一彦,堀 克敏,丹治保典,海野 肇

    化学工学会第66回年会講演要旨集, P109 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東広島   Country:Japan  

  403. 塩化ビニル製造における塩素のフロー

    丹治保典,宮永一彦,堀 克敏,海野 肇

    化学工学会第66回年会講演要旨集, F107 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東広島   Country:Japan  

  404. 担体・膜ハイブリッド型廃水処理システムの性能

    一戸里江子,宮永一彦,堀 克敏,丹治保典,海野 肇

    化学工学会神奈川大会要旨集,D206 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜   Country:Japan  

  405. Acinetobacter genospecies Tol 5のトルエン分解とポリウレタン担体付着特性

    石井俊一,堀 克敏,山下茂樹,北川正美,丹治保典,海野 肇

    化学工学会神奈川大会要旨集,D205 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜   Country:Japan  

  406. バイオフィルム内微生物に対するバイオサイドの抗菌活性

    中野 肇,丹治保典,堀 克敏,宮永一彦,海野 肇

    材料と環境2001講演要旨集, A-106S 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:つくば   Country:Japan  

  407. 担体・膜ハイブリッド型システムにおける有機質および窒素除去性能の解析

    宮永一彦,一戸里江子,船田茂行,堀 克敏,丹治保典,海野 肇

    化学工学会第34回秋季大会講演要旨集, Q208 

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    Event date: 2001

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌   Country:Japan  

  408. Isolation, characterization and application to off-gas treatment of toluene degrading bacteria International conference

    K. Hori, S. Yamashita, S. Ishii, M. Kitagawa, Y. Tanji, H. Unno

    The 1st Joint China/Japan Chemical Engineering Symposium, proc. 269-274 

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    Event date: 2000

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  409. Isolation and characterization of the novel toluene degrading bacteria which has high hydrophobicity International conference

    S. Ishii, K. Hori, S. Yamashita, M. Kitagawa, S. Yamamoto, Y. Tanji, H. Unno

    th International Symposium on Environmental Biotechnology, proc. 793-796 

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    Event date: 2000

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  410. Comparison of the degradation pattern and rate of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in water and soil by bacteria, actinomycetes and fungi isolated from soil International conference

    B.-I. Sang, K. Hori, S. Yamamoto, Y. Tanji, H. Unno

    5th International Symposium on Environmental Biotechnology, proc. 985-988 

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    Event date: 2000

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  411. Analysis of microbial community in the degradation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in soil International conference

    B.-I. Sang, K. Hori, S. Yamamoto, Y. Tanji, H. Unno

    5th International Symposium on Environmental Biotechnology, proc. 981-984 

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    Event date: 2000

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  412. Effect of the manipulation for dispersion of oil phase on the bioavailability and degradation of a solid alkane dissolved in the model oil International conference

    K. Hori, Y. Matsuzaki, S. Yamamoto, Y. Tanji, H. Unno

    5th International Symposium on Environmental Biotechnology, proc. 309-312 

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    Event date: 2000

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  413. 分散操作が油滴中に溶解した固体アルカンの微生物分解に及ぼす影響

    堀 克敏、松崎由布子、丹治保典、海野 肇

    化学工学会第33回秋季大会講演要旨集, Z326 

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    Event date: 2000

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:静岡   Country:Japan  

  414. 浄化槽汚泥内ファージの季節変動と環境条件がファージ濃度に及ぼす影響の解析

    丹治保典,溝口克典,堀 克敏,海野 肇

    第34回日本水環境学会年会講演要旨集, 133 

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    Event date: 2000

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都   Country:Japan  

  415. 混合培養系におけるファージと宿主の相互作用の解析

    溝口克典,丹治保典,堀 克敏,海野 肇

    第34回日本水環境学会年会講演要旨集, 132 

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    Event date: 2000

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都   Country:Japan  

  416. 塩化ビニル製造における塩素フローの解析と塩素利用の適正化

    宮永一彦,丹治保典,堀 克敏,海野 肇

    環境科学会2000年年会講演要旨集, 2P03 

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    Event date: 2000

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:浦安   Country:Japan  

  417. 自己溶菌性大腸菌による外来タンパク質の生産とその回収

    森田昌知,浅見和広,丹治保典,山本進二郎,堀 克敏,海野 肇

    化学工学会第65回年会講演要旨集, I102 

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    Event date: 2000

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  418. 浄化槽汚泥内ファージの季節変動と環境条件がファージ濃度に及ぼす影響の解析

    丹治保典,溝口克典,堀 克敏,山本進二郎,海野 肇

    化学工学会第65回年会講演要旨集, J322 

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    Event date: 2000

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  419. P(3HB-co-3HV) の土壌中での分解過程における微生物コンソーシアの解析

    B.-I. Sang, 堀 克敏,山本進二郎,丹治保典,海野 肇

    化学工学会第65回年会講演要旨集, J102 

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    Event date: 2000

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京   Country:Japan  

  420. 人口微生物膜を用いた金属腐食の解析

    丹治保典,伊藤鉄平,堀 克敏,海野 肇

    生物工学会平成12年度大会要旨集, 701 

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    Event date: 2000

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:札幌   Country:Japan  

  421. 炭素鋼腐食を促進する微生物膜の解析

    丹治保典,伊藤鉄平,堀 克敏,海野 肇

    材料と環境 2000講演要旨集, A-110 

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    Event date: 2000

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:横浜   Country:Japan  

  422. タバコ培養細胞による大麦レクチンの分泌生産

    丹治保典,石原明日子,若尾節子,ケイ新会,堀 克敏,海野 肇

    化学工学会第33回秋季大会講演要旨集, Z132 

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    Event date: 2000

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:静岡   Country:Japan  

  423. Effect of low oxygen tension on phenotype reexpression of chondrocytes

    山本進二郎,村上哲也,堀 克敏,丹治保典,海野 肇

    化学工学会第33回秋季大会講演要旨集, Z130 

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    Event date: 2000

    Language:English   Presentation type:Oral presentation (general)  

    Venue:静岡   Country:Japan  

  424. Simultaneous removal of carbonaceous and nitrogenous compounds by porous carrier and membrane hybrid process

    W. M. G. C. Kumari, R. Ichinohe, K. Miyanaga, K. Hori, Y. Tanji, H. Unno

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    Event date: 2000

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  425. 非分解性オイル相に溶解した疎水性基質によるバクテリアの増殖と基質取り込み過程の解析

    堀 克敏,崔 東赫,丹治保典,海野 肇

    生物工学会平成10年度大会 

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    Event date: 1999.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:広島   Country:Japan  

  426. Profile of microbial community change during microbial degradation of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) in soil International conference

    K. Hori, B.-I. Sang, Y. Tanji, H. Unno

    5th Asia-Pacific Biochemical Engineering Conference, proc. P-ENV33 

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    Event date: 1999

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  427. Development of integrated lectin production process by extracellular targeting of produced protein in plant cell International conference

    A. Ishihara, S. Wakao, Y. Tanji, K. Hori, S. Yamamoto, X.-h. Xing, H. Unno

    th Asia-Pacific Biochemical Engineering Conference, P-PB3 

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    Event date: 1999

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  428. Effects of biofilm structure on the metal corrosion International conference

    Y. Morono, A. Soejima, S. Yamamoto, K. Hori, Y. Tanji, H. Unno

    th Asia-Pacific Biochemical Engineering Conference, P-ENV14 

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    Event date: 1999

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  429. Construction of dual vector system for recovering of intracellular proteins International conference

    M. Morita, K. Asami, Y. Tanji, S. Yamamoto, K. Hori, H. Unno

    th Asia-Pacific Biochemical Engineering Conference, P-RM15 

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    Event date: 1999

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  430. Characterization of phage lysozymes and their application to antimicrobial agents International conference

    A. Soejima, A. Muyombwe, Y. Tanji, K. Hori, S. Yamamoto, H. Unno

    5th Asia-Pacific Biochemical Engineering Conference, P-RM16 

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    Event date: 1999

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  431. Enzymatic hydrolysis of β-lactoglobulin by trypsin covalently immobilized onto cellulosic support International conference

    A. Imamura, I. Susanti, K. Hori, S. Yamamoto, Y. Tanji, H. Unno

    5th Asia-Pacific Biochemical Engineering Conference, P-ET12 

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    Event date: 1999

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  432. Antialgal activity of Chlorella virus-encoded novel alginate lyase International conference

    Y. Tanji, K. Suda, M. Yamaji, K. Hori, S. Yamamoto, H. Unno

    th Asia-Pacific Biochemical Engineering Conference, P-RM19 

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    Event date: 1999

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  433. Akinete formation of Anabaena cylindrica -controlling factors for the formation and its contribution to the water International conference

    S. Ishii, K. Hori, Y. Tanji, C. Weeraphasphong, H. Unno

    5th Asia-Pacific Biochemical Engineering Conference, proc. P-ENV14 

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    Event date: 1999

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  434. Purification of phage lysozymes and their exogenous effects on bacterial growth

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    Event date: 1999

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  435. バイオフィルム形成による炭素鋼腐食の解析

    諸野祐樹,副島 綾,堀 克敏,海野 肇

    化学工学会第64回年会講演要旨集, G306 

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    Event date: 1999

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  436. ファージを介した遺伝子伝達に及ぼす環境因子の解析 International conference

    丹治保典,柳田真里,堀 克敏,海野 肇

    化学工学会第64回年会講演要旨集, D303 

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    Event date: 1999

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  437. 非分解性オイル相に溶解させた固体アルカンの微生物分解過程の解析

    崔 東赫,堀 克敏,丹治保典,海野 肇

    化学工学会第64回年会講演要旨集, D216 

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    Event date: 1999

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:名古屋   Country:Japan  

  438. クロレラウイルスの スクリーニングとその溶菌酵素の解析

    丹治保典,須田幸治,山地まき,堀 克敏,海野 肇

    生物工学会平成11年度大会講演要旨集, 746 

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    Event date: 1999

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  439. 炭素鋼腐食を促進する微生物膜の解析

    丹治保典,諸野祐樹,副島 綾,堀 克敏,山本進二郎,海野 肇

    生物工学会平成11年度大会講演要旨集,1212 

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    Event date: 1999

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  440. Acinetobacter 属細菌による油相中に溶解したアルカンの分解過程の解析

    堀 克敏,崔 東赫,松崎由布子,丹治保典,山本進二郎,海野 肇

    生物工学会平成11年度大会講演要旨集, 1001 

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    Event date: 1999

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  441. n-アルカンの微生物分解における基質取り込み過程の解析

    松崎由布子,丹治保典,堀 克敏,山本進二郎,海野 肇

    化学工学会第32回秋季大会講演要旨集, E108 

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    Event date: 1999

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:金沢   Country:Japan  

  442. ヒト由来細菌に感染するファージの浄化槽汚泥内消長

    丹治保典,溝口克典,堀 克敏,山本進二郎,海野 肇

    化学工学会第32回秋季大会講演要旨集, E107 

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    Event date: 1999

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:金沢   Country:Japan  

  443. Dual expression system for the production of β-glucuronidase in Escherichia coli and release into the medium

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    Event date: 1999

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

  444. 塩化ビニル生産プロセスにおける塩素化合物フローの解析

    堀 克敏,丹治保典,海野 肇

    環境科学会1998年会 

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    Event date: 1998.10

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:つくば   Country:Japan  

  445. 炭、微生物の複合機能による水質浄化システムの開発

    堀 克敏,梶原 将,太田口和久

    化学工学会第63回年会講演要旨集, D301 

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    Event date: 1998

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:大阪   Country:Japan  

  446. Hydrolysis of β-lactoglobulin by trypsin immobilized on a cellulose support International conference

    A. Imamura, S. Yamamoto, K. Hori, Y. Tanji, H. Unno

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    Event date: 1998

    Language:English   Presentation type:Oral presentation (general)  

    Country:Japan  

▼display all

Works 30

  1. 微生物 油分解し排水処理

    2023.7

  2. 微生物と酵素でSDGs 加速目指し産学連携拡大

    2023.6

  3. くらしの中から考える マスクどうする?

    2022.9

  4. ウイルス不活性化装置開発 名大発ベンチャーのフレンドマイクローブ 地元企業と連携 フィルター不要で除菌

    2022.1

  5. マスクの効果 顔隙間で半減

    2021.6

  6. マスクと併用し装着 カメの形「タートル」

    2021.6

  7. コロナ対応で「3Dマスク」開発

    2020.7

  8. 再使用可能マスク開発へ 名大発新興 3Dプリンター活用

    2020.5

  9. 3Dプリンターで立体マスク 再使用可能 一部不織布 名古屋大など開発 秋にも発売

    2020.5

  10. 繰り返し使えるプラ製マスク 名大など開発 夏に試験販売

    2020.5

  11. 工場排水処理で名大ベンチャー 微生物で油分迅速分解

    2017.1

  12. 「微生物で排水処理」名古屋大がベンチャー設立へ

    2017.1

  13. 微生物細胞の毛 無傷で刈れる

    2016.6

  14. 名大 油分解の微生物製剤 性能実証デモ機完成

    2015.9

  15. 強力にエコに排水の油分解

    2015.8

  16. 粘着性たんぱく質発見

    2013.1

  17. 微生物に「毛」を生やす 名大教授 たんぱく質発見

    2012.11

  18. 接着力のあるたんぱく質発見

    2012.11

  19. 名大と帝人、炭素繊維が微生物を集めて水を浄化するメカニズムを解明

    2012.9

  20. 炭素繊維の水浄化解明

    2012.8

  21. 調理用排水簡易浄化を実現

    2009.9

  22. 細菌で油成分効果的に分解

    2009.7

  23. 飲食店の廃油微生物分解

    2009.7

  24. 調理場から出る油も効率的に分解

    2009.7

  25. 排水中の油を分解

    2009.7

  26. 粘着微生物 構造・機能を解析 ナノテクと融合、技術創出

    2007.5

  27. 環境にやさしいプラスチックは微生物が作る時代

    2002.8

  28. 生分解性プラスチックと微生物界面活性剤を同時につくってコストを削減

    2002.5

  29. 生分解性プラと界面活性剤 生産菌も効率培養 東工大

    2002.5

  30. 東工大、生分解性プラスチックと界面活性剤を微生物を使って同時生産するプロセス開発

    2002.5

▼display all

Research Project for Joint Research, Competitive Funding, etc. 18

  1. 雑食性微生物を用いた物質生産のプラットフォーム構築

    2023.10 - 2025.3

    革新的GX技術創出事業  GteX

    堀 克敏

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    Grant amount:\20150000 ( Direct Cost: \14105000 、 Indirect Cost:\6045000 )

  2. 安全なウイルス研究・創薬プラットホーム:ウイルス様粒子の超迅速作製システムの構築に関する研究開発

    2020.9 - 2021.3

    ウイルス等感染症対策技術開発事業  受託研究

    堀 克敏

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\35850000 ( Direct Cost: \23500000 、 Indirect Cost:\7050000 )

  3. 微生物パワーによる次世代閉鎖循環式陸上養殖システムの構築

    2018.11 - 2021.3

    未来社会創造事業  受託研究

    堀 克敏

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\25850500 ( Direct Cost: \19885000 、 Indirect Cost:\5965500 )

  4. アクリル酸、メタクリル酸に誘導可能な化合物を産生する細菌の遺伝子改変、ならびに気相生物リアクターの研究

    2023.11 - 2024.10

    共同研究

    堀 克敏

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    Grant amount:\7380000 ( Direct Cost: \5000000 、 Indirect Cost:\1500000 )

  5. 魚類体表微生物叢の網羅的解析と感染予防効果の検討:抗生物質を代替する養殖魚類表皮版プロバイオティクスの確立を目指して

    2017.4 - 2019.3

    発酵研究所大型研究 

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    Grant type:Competitive

  6. 油脂分解微生物を利用する低コスト・ハイパフォーマンス排水処理システム

    2014.12 - 2017.3

    A-STEP 

    堀 克敏

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    Grant type:Competitive

  7. 気相微生物反応を用いる革新的バイオプロセスによるメタン/メタノール変換

    2014.10 - 2020.3

    ALCA(先端的低炭素技術開発) 

    堀 克敏

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    Grant type:Competitive

  8. ケミカル-低炭素生産を可能とするバイオ化学工業プロセスの実現のための革新的微生物利用技術開発研究

    2011.3 - 2012.9

    ALCA 

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    Grant type:Competitive

  9. Construction of interfacial microbial processes based on the function of bacterionanofiber poteins

    2011.2 - 2014.3

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    Grant type:Competitive

  10. 食品廃棄物からの高活性・高安定性厨房排水処理用バイオ製剤の効率的生産プロセスの開発

    2010.9 - 2012.3

    戦略的基盤技術高度化支援事業 

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    Grant type:Competitive

  11. ボトムアップ型油脂分解バイオフィルムによる厨房排水処理技術の実用化

    2010.4 - 2011.3

    次世代最先端技術人材育成事業 

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    Grant type:Competitive

  12. 微生物による油脂分解複合効果の検証と解析

    2010.1 - 2010.12

    企業研究者活用型基礎研究推進事業 

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    Grant type:Competitive

  13. バイオフィルムによるグリーストラップ浄化技術の実用化に向けた遊離脂肪酸分解除去システムの確立

    2009.4 - 2011.3

    科学技術交流財団 

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    Grant type:Competitive

  14. 厨房排水処理用油脂分解バイオフィルムの高機能化・安定化のための微生物製剤導入技術の研究開発事業

    2007.4 - 2010.3

    「微生物群デザイン化による高効率型環境バイオ処理技術開発」プロジェクト 

      More details

    Grant type:Competitive

  15. 細菌ナノファイバーの構造と接合界面の制御

    2006.10 - 2010.3

    戦略的創造研究推進事業 さきがけ 

    堀 克敏

      More details

    Grant type:Competitive

  16. 機能性バイオフィルムによる設計・制御技術によるグリーストラップのクリーン化

    2006.4 - 2007.3

    シーズ発掘試験研究 

    堀 克敏

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    Grant type:Competitive

  17. ものづくりバイオによる医療・健康工学のための基盤技術の確率

    2006.4 - 2007.3

    出資金による受託研究 

  18. 有機塩素化合物の微生物分解

    2000.4 - 2003.3

    出資金による受託研究 

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KAKENHI (Grants-in-Aid for Scientific Research) 14

  1. 完全再構成型ウイルス・細菌様粒子のボトムアップ構築

    Grant number:21H05227  2021.9 - 2026.3

    科学研究費助成事業  学術変革領域研究(A)

    堀 克敏

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    Authorship:Principal investigator 

    Grant amount:\158340000 ( Direct Cost: \121800000 、 Indirect Cost:\36540000 )

  2. ナノファイバータンパク質の分子紡績

    Grant number:21H01727  2021.4 - 2024.3

    日本学術振興会  科学研究費補助金  基盤研究(B)

    石川 聖人

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\3250000 ( Direct Cost: \2500000 、 Indirect Cost:\750000 )

  3. Elucidation of the mechanism in disease protection of the fish epidermis-derived probiotics.

    Grant number:20H02539  2020.4 - 2023.3

    JSPS  Grant-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research(B)

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\3250000 ( Direct Cost: \2500000 、 Indirect Cost:\750000 )

  4. Elucidation of nano-mechanical properties of a highly adhesive and highly extensible protein and creation of mechanochemical materials

    Grant number:20H00319  2020.4 - 2023.3

    JSPS  Grant-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research(A)

      More details

    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\45240000 ( Direct Cost: \34800000 、 Indirect Cost:\10440000 )

  5. Development of surface modification technology using a bacterial adhesive protein International coauthorship

    Grant number:17H01345  2017.4 - 2020.3

    JSPS  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (A)

    Hori Katsutoshi

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    Authorship:Principal investigator  Grant type:Competitive

    Grant amount:\44590000 ( Direct Cost: \34300000 、 Indirect Cost:\10290000 )

    A rapid and versatile immobilization method using an adhesive protein for peptides, proteins, and liposomes onto material surfaces was developed. Furthermore, we clarified the effects of the adhesive protein-coated surfaces on cell adhesion and proliferation. The adhesion of the adhesive protein to the material surface was observed in water by AFM, measured by quartz crystal microbalance (QCM), and analyzed by all-atom molecular dynamics (MD) calculations to gain insight into the adhesion modes.

  6. Microorganisms in a Confined Environment

    Grant number:15H05946  2015.6 - 2020.3

    Grant-in-Aid for Scientific Research   Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

    Nasu Masao

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\9989304 ( Direct Cost: \7684080 、 Indirect Cost:\2305224 )

    In a confined environment, environmental oligotrophic bacteria remain active on the wall surface, while human-derived bacteria showed high physiological activity in the air. Due to stress found in a confined environment, microbial substitutions among Malassezia species, the predominant skin microbiome, were observed. This suggests that Malassezia could be a stress marker.
    We demonstrated that the initial process of the biofilm formation under the microgravity is different from that under the normal gravity; the cell aggregate is formed before initial attachment on the material surface. It was also suggested that the frequency of gene transfer by conjugation was increased under simulated microgravity.

  7. 機能性バクテリオナノファイバーのデザインと被毛微生物の作出

    Grant number:26289313  2014.4 - 2017.3

    科学研究費補助金  基盤研究(B)

    堀 克敏

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    Authorship:Principal investigator 

  8. リポソームから毛を生やす

    Grant number:26630426  2014.4 - 2016.3

    科学研究費補助金 

    堀 克敏

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    Authorship:Principal investigator 

  9. 接着性バクテリオナノファイバー蛋白質を利用する微生物細胞表層の高機能化

    Grant number:23360368  2011.4 - 2012.3

    科学研究費補助金  基盤研究(B)

    堀 克敏

      More details

    Authorship:Principal investigator 

  10. バイオフィルム形成初期過程の制御に向けた細菌付着のナノメカニズム

    Grant number:16360409  2004.4 - 2007.3

    科学研究費補助金  基盤研究(B)

    堀 克敏

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    Authorship:Principal investigator 

  11. 疎水性物質との相互作用に関わる環境汚染物質分解菌表層分子機構の解明とその応用

    Grant number:14045222、13031027  2001.4 - 2004.3

    科学研究費補助金  特定領域研究

    堀 克敏

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    Authorship:Principal investigator 

  12. プログラム自己破砕細菌による生分解性プラスチックの高度生産・分離プロセスの開発

    Grant number:11695041  1999.4 - 2002.3

    科学研究費補助金  基盤研究(B)

    海野 肇

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    Authorship:Coinvestigator(s) 

  13. ファージによるバイオレメディエーションのための遺伝子ターゲティング

    Grant number:11132217  1999.4 - 2000.3

    科学研究費補助金  特定領域研究

    堀 克敏

      More details

    Authorship:Principal investigator 

  14. 生分解性ポリマーのオンサイト分析機構および分解形態とその特性に関する研究

    Grant number:10450307  1998.4 - 2001.3

    科学研究費補助金  基盤研究(B)

    海野 肇

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    Authorship:Coinvestigator(s) 

▼display all

Industrial property rights 62

  1. 油含有排水処理のための微生物製剤の連続投入

    堀克敏

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    Applicant:東海国立大学機構

    Application no:2021-68159  Date applied:2021.4

    Patent/Registration no:6989987  Date registered:2021.12  Date issued:2022.2

    Rights holder:名古屋大学

  2. PCT)気相微生物反応

    堀克敏、チェンヤンユー

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    Applicant:東海国立大学機構

    Application no:PCT/JP2021/015227  Date applied:2021.4

    Announcement no:WO2021/210553  Date announced:2021.10

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  3. 油脂含有排水処理方法、システムおよび装置

    堀克敏

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    Applicant:東海国立大学機構

    Application no:2021-032846  Date applied:2021.3

    Announcement no:2021-100758  Date announced:2021.7

    Country of applicant:Domestic   Country of acquisition:Domestic

  4. 脂肪酸含有油脂を分解する新規微生物

    堀克敏

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    Applicant:東海国立大学機構

    Application no:2020-158725  Date applied:2020.9

    Announcement no:2021-000124  Date announced:2021.1

    Country of applicant:Domestic   Country of acquisition:Domestic

  5. トランス脂肪酸含有エステル体を分解する新規エステラーゼ

    堀克敏

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    Applicant:東海国立大学機構

    Date applied:2020.9

    Announcement no:WO2021/045234  Date announced:2021.3

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  6. PCT)低温で高い油脂分解能力を有する新規微生物

    堀克敏

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    Applicant:東海国立大学機構

    Application no:PCT/JP2020/033836  Date applied:2020.9

    Announcement no:WO2021/045235  Date announced:2021.3

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  7. PCT)接着タンパク質

    堀克敏

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    Applicant:東海国立大学機構

    Application no:PCT/JP2020/027921  Date applied:2020.7

    Announcement no:WO2021/010481  Date announced:2021.1

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  8. PCT)分離ペプチド

    堀 克敏

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    Applicant:東海国立大学機構

    Date applied:2020.7

    Announcement no:WO2021/010482  Date announced:2021.1

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  9. JP)接着タンパク質

    堀克敏

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    Applicant:東海国立大学機構

    Application no:2021-533117  Date applied:2020.7

    Announcement no:WO2021/010481  Date announced:2021.1

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  10. US)分離ペプチド

    堀 克敏

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    Applicant:東海国立大学機構

    Application no:17/682.142  Date applied:2020.7

    Announcement no:WO2021/010482  Date announced:2021.1

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  11. JP)分離ペプチド

    堀 克敏

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    Applicant:東海国立大学機構

    Application no:2021-533118  Date applied:2020.7

    Announcement no:WO2021/010482  Date announced:2021.1

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  12. PCT)魚類表皮プロバイオティクスに有用な微生物

    堀克敏

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    Applicant:東海国立大学機構

    Application no:PCT/JP2020/022437  Date applied:2020.6

    Announcement no:WO2020/246609  Date announced:2020.12

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  13. JP)魚類表皮プロバイオティクスに有用な微生物

    堀克敏

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    Applicant:東海国立大学機構

    Application no:2021-524942  Date applied:2020.6

    Announcement no:WO2020/246609  Date announced:2020.12

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  14. US)トランス脂肪酸含有油脂を分解する新規リパーゼ

    堀克敏

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    Applicant:東海国立大学機構

    Application no:US,201917258127  Date applied:2019.7

    Announcement no:US.2021/0277369  Date announced:2021.9

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  15. US)脂肪酸含有油脂を分解する新規微生物

    堀克敏

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    Applicant:東海国立大学機構

    Application no:US.2019.17258132  Date applied:2019.7

    Announcement no:US.2021/0276904  Date announced:2021.9

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  16. EP)トランス脂肪酸含有油脂を分解する新規リパーゼ

    堀 克敏

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    Applicant:東海国立大学機構

    Application no:EP.1982980  Date applied:2019.7

    Announcement no:EP.3819379  Date announced:2021.5

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  17. EP)脂肪酸含有油脂を分解する新規微生物

    堀克敏

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    Applicant:東海国立大学機構

    Application no:EP.19830561  Date applied:2019.7

    Announcement no:EP.3819371  Date announced:2021.5

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  18. CN)トランス脂肪酸含有油脂を分解する新規リパーゼ

    堀克敏

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    Applicant:東海国立大学機構

    Application no:CN.201980058385  Date applied:2019.7

    Announcement no:CN.112654708  Date announced:2021.4

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  19. PCT)脂肪酸含有油脂を分解する新規微生物

    堀克敏

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    Applicant:東海国立大学機構

    Application no:PCT/JP2019/026850  Date applied:2019.7

    Announcement no:WO2020/009232  Date announced:2020.1

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  20. PCT)トランス脂肪酸含有油脂を分解する新規リパーゼ

    堀克敏

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    Applicant:東海国立大学機構

    Application no:PCT/JP2019/026848  Date applied:2019.7

    Announcement no:WO2020/009231  Date announced:2020.1

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  21. JP)トランス脂肪酸含有油脂を分解する新規リパーゼ

    堀克敏

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    Applicant:東海国立大学機構

    Application no:2020-529069  Date applied:2019.7

    Announcement no:WO2020/009231  Date announced:2020.1

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  22. EP)油脂含有排水処理方法、システムおよび装置

    堀克敏

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    Applicant:東海国立大学機構

    Application no:EP.18878958  Date applied:2018.11

    Announcement no:EP.3712247  Date announced:2020.9

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  23. 油脂含有排水処理方法、システムおよび装置

    堀克敏

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    Applicant:東海国立大学機構

    Application no:2019-540014  Date applied:2018.11

    Announcement no:WO2019/098255  Date announced:2019.5

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  24. 抽出物、可溶性画分、タンパク質、バイオフィルムを分解する方法

    岡村 大祐、堀 克敏

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    Applicant:旭化成株式会社、東海国立大学機構、名古屋大学

    Application no:2018-133251  Date applied:2018.7

    Announcement no:2020-011911  Date announced:2020.1

    Country of applicant:Domestic   Country of acquisition:Domestic

  25. 脂肪酸含有油脂を分解する新規微生物

    堀 克敏

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    Application no:2019-557658  Date applied:2018.7

    Announcement no:WO2020/009232  Date announced:2020.1

    Country of applicant:Domestic  

  26. 接着性ファイバー及びその用途

    堀 克敏

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    Applicant:東海国立大学機構、名古屋大学

    Application no:2016-036380  Date applied:2016.2

    Announcement no:2017-149703  Date announced:2017.8

    Patent/Registration no:6795138  Date registered:2020.11 

    Country of applicant:Domestic   Country of acquisition:Domestic

  27. 微生物の着脱及び脱離方法

    堀 克敏

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    Applicant:東海国立大学機構、名古屋大学

    Application no:2015-181162  Date applied:2015.9

    Announcement no:2017-055676  Date announced:2017.3

    Patent/Registration no:6610868  Date registered:2015.11 

    Country of applicant:Domestic   Country of acquisition:Domestic

  28. PCT)被毛微生物

    堀 克敏

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    Applicant:東海国立大学機構、名古屋大学

    Application no:PCT/JP2014/072735  Date applied:2014.8

    Announcement no:WO2015/030171  Date announced:2015.3

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  29. JP)被毛微生物

    堀 克敏

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    Applicant:東海国立大学機構、名古屋大学

    Application no:2015-531325  Date applied:2014.8

    Announcement no:WO2015/030171  Date announced:2015.3

    Patent/Registration no:6562512  Date registered:2019.8 

    Country of applicant:Domestic  

  30. JP)微生物の固定化および脱離方法

    堀 克敏

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    Applicant:名古屋大学

    Application no:2015-508308  Date applied:2014.3

    Announcement no:WO2014/156736  Date announced:2017.2

    Patent/Registration no:6353438  Date registered:2018.6 

    Country of applicant:Domestic  

  31. US)微生物の固定化及び脱離方法

    堀 克敏

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    Application no:US201414779717  Date applied:2014.3

    Announcement no:US2016/0046923  Date announced:2016.2

    Patent/Registration no:US9631189 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  32. EP)微生物の固定化及び脱離方法

    堀 克敏

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    Applicant:東海国立大学機構、名古屋大学

    Application no:EP14773956  Date applied:2014.3

    Announcement no:EP2980211  Date announced:2016.2

    Patent/Registration no:EP2980211 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  33. PCT)微生物の固定化及び脱離方法

    堀 克敏

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    Applicant:東海国立大学機構、名古屋大学

    Application no:PCT/2014/056966  Date applied:2014.3

    Announcement no:WO2014/156736  Date announced:2014.10

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  34. キメラ被毛微生物

    堀 克敏

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    Applicant:東海国立大学機構、名古屋大学

    Application no:2013-179950  Date applied:2013.8

    Announcement no:2015-047107  Date announced:2015.3

    Patent/Registration no:6233765  Date registered:2017.11 

    Country of applicant:Domestic   Country of acquisition:Domestic

  35. 嫌気性アンモニア酸化細菌の検出方法

    黒住 明大、渡邊 哲文、高瀬 長武、福崎 康博、川久保 祐貴、中村 安宏、堀 克敏

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    Applicant:株式会社明電舎、東海国立大学機構、名古屋大学

    Application no:2013-120305  Date applied:2013.6

    Announcement no:2014-236685  Date announced:2014.12

    Patent/Registration no:6138597  Date registered:2017.5 

    Country of applicant:Domestic   Country of acquisition:Domestic

  36. SG)新規ヤロウィア属微生物、並びにそれを用いた油分解剤及び油分解除去方法

    堀 克敏、藤岡 正剛

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    Applicant:日産化学工業株式会社、名古屋大学、株式会社フジミックス

    Application no:SG11201404183  Date applied:2013.1

    Announcement no:SG11201414183  Date announced:2016.9

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  37. MY)新規ヤロウィア属微生物、並びにそれを用いた油分解剤及び油分解除去方法

    堀 克敏、藤岡 正剛

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    Applicant:日産化学工業株式会社、名古屋大学、株式会社フジミックス

    Application no:MYPI2014002113  Date applied:2013.1

    Announcement no:MY164111  Date announced:2015.9

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  38. US)新規ヤロウィア属微生物、並びにそれを用いた油分解剤及び油分解除去方法

    堀 克敏、藤岡 正剛

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    Applicant:日産化学工業株式会社、名古屋大学、株式会社フジミックス

    Application no:US20131437831  Date applied:2013.1

    Announcement no:US2015/0024470  Date announced:2015.1

    Patent/Registration no:US10055877 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  39. EP)新規ヤロウィア属微生物、並びにそれを用いた油分解剤及び油分解除去方法

    堀 克敏、藤岡 正剛

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    Applicant:日産化学工業株式会社、名古屋大学、株式会社フジミックス

    Application no:EP13738396  Date applied:2013.1

    Announcement no:EP2816015  Date announced:2014.12

    Patent/Registration no:EP2816015 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  40. KR)新規ヤロウィア属微生物、並びにそれを用いた油分解剤及び油分解除去方法

    堀 克敏、藤岡 正剛

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    Applicant:日産化学工業株式会社、名古屋大学、株式会社フジミックス

    Application no:KR20147022895  Date applied:2013.1

    Announcement no:KR20140116207  Date announced:2014.10

    Patent/Registration no:KR102026750 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  41. CN)新規ヤロウィア属微生物、並びにそれを用いた油分解剤及び油分解除去方法

    堀 克敏、藤岡 正剛

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    Applicant:日産化学工業株式会社、名古屋大学、株式会社フジミックス

    Application no:CN2013380005842  Date applied:2013.1

    Announcement no:CN104053633  Date announced:2014.9

    Patent/Registration no:ZL201380005842.X 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  42. PCT)新規ヤロウィア属微生物、並びにそれを用いた油分解剤及び油分解除去方法

    堀 克敏、藤岡 正剛

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    Applicant:日産化学工業株式会社、名古屋大学、株式会社フジミックス

    Application no:PCT/JP2013/050649  Date applied:2013.1

    Announcement no:WO2013/108775  Date announced:2013.7

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  43. JP)新規ヤロウィア属微生物、並びにそれを用いた油分解剤及び油分解除去方法

    堀 克敏、藤岡 正剛

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    Applicant:日産化学工業株式会社、名古屋大学、株式会社フジミックス

    Application no:2012-009451  Date applied:2012.1

    Announcement no:2013-146689  Date announced:2013.8

    Patent/Registration no:05685783  Date registered:2015.1 

    Country of applicant:Domestic   Country of acquisition:Domestic

  44. バイオフィルム除去剤

    岡村 大祐、堀 克敏

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    Applicant:東海国立大学機構、旭化成株式会社、旭化成ケミカルズ株式会社、名古屋大学、 名古屋工業大学

    Application no:2011-081601  Date applied:2011.4

    Announcement no:2012-213364  Date announced:2012.11

    Patent/Registration no:5856749  Date registered:2015.12 

    Country of applicant:Domestic   Country of acquisition:Domestic

  45. TW)膜分離活性汚泥法に用いる添加剤

    岡村大祐、堀克敏

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    Applicant:国立大学法人東海国立大学機構 旭化成株式会社 旭化成ケミカルズ株式会社 国立大学法人名古屋大学 国立大学法人 名古屋工業大学

    Date applied:2010.10

    Announcement no:TW201129510  Date announced:2011.9

    Patent/Registration no:I415803 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  46. JP)膜分離活性汚泥法に用いる添加剤

    岡村大祐、堀克敏

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    Applicant:国立大学法人東海国立大学機構 旭化成株式会社 旭化成ケミカルズ株式会社 国立大学法人名古屋大学 国立大学法人 名古屋工業大学

    Application no:2011-535357  Date applied:2010.9

    Announcement no:WO2011/043232  Date announced:2013.3

    Publication no:2011043232 

    Patent/Registration no:5788327  Date registered:2015.8 

    Country of applicant:Domestic  

  47. EP)膜分離活性汚泥法に用いる添加剤

    岡村大祐、堀克敏

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    Applicant:国立大学法人東海国立大学機構 旭化成株式会社 旭化成ケミカルズ株式会社 国立大学法人名古屋大学 国立大学法人 名古屋工業大学

    Application no:EP10821903  Date applied:2010.9

    Announcement no:EP2487139  Date announced:2012.8

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  48. CN)膜分離活性汚泥法に用いる添加剤

    岡村大祐、堀克敏

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    Applicant:国立大学法人東海国立大学機構 旭化成株式会社 旭化成ケミカルズ株式会社 国立大学法人名古屋大学 国立大学法人 名古屋工業大学

    Application no:CN201080044641  Date applied:2010.9

    Announcement no:CN104053633  Date announced:2012.7

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  49. US)膜分離活性汚泥法に用いる添加剤

    岡村大祐、堀克敏

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    Applicant:国立大学法人東海国立大学機構 旭化成株式会社 旭化成ケミカルズ株式会社 国立大学法人名古屋大学 国立大学法人 名古屋工業大学

    Application no:US2010800446  Date applied:2010.9

    Announcement no:US201013393856  Date announced:2012.6

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  50. PCT)膜分離活性汚泥法に用いる添加剤

    岡村大祐、堀克敏

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    Applicant:国立大学法人東海国立大学機構 旭化成株式会社 旭化成ケミカルズ株式会社 国立大学法人名古屋大学 国立大学法人 名古屋工業大学

    Application no:PCT/JP2010/066947  Date applied:2010.9

    Announcement no:WO2011/043232  Date announced:2011.4

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  51. マイクロバブル/ナノバブルによる油の微生物分解処理の促進法

    堀克敏

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    Applicant:名古屋大学 名古屋工業大学 株式会社フジミックス

    Application no:2009-133438  Date applied:2009.6

    Announcement no:2010-279862  Date announced:2010.12

    Country of applicant:Domestic   Country of acquisition:Domestic

  52. リパーゼまたはその分泌微生物と加水分解生成物分解微生物との複合効果による油脂含有排水の処理方法とグリーストラップ浄化方法及び油脂分解剤

    堀克敏

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    Applicant:日産化学工業株式会社 名古屋大学 名古屋工業大学

    Application no:2009-79299  Date applied:2009.3

    Announcement no:2010-227849  Date announced:2010.10

    Patent/Registration no:5640211  Date registered:2014.11 

    Country of applicant:Domestic   Country of acquisition:Domestic

  53. 弱酸性条件で増殖・油脂分解可能なリパーゼ分泌微生物による油脂含有排水の処理方法とグリーストラップ浄化方法及び油脂分解剤

    堀克敏

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    Applicant:日産化学工業株式会社 名古屋大学  名古屋工業大学

    Application no:2009-079432  Date applied:2009.3

    Announcement no:2010-227858  Date announced:2010.10

    Patent/Registration no:05470614  Date registered:2014.2 

    Country of applicant:Domestic   Country of acquisition:Domestic

  54. バイオフィルムによる油脂分解とオゾンによる脱臭を両立させるグリーストラップの衛生化技術

    堀克敏

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    Applicant:名古屋工業大学 株式会社マルタカ電器

    Application no:2008-065114  Date applied:2008.3

    Announcement no:2008-253989  Date announced:2008.10

    Patent/Registration no:05046121  Date registered:2012.7 

    Country of applicant:Domestic   Country of acquisition:Domestic

  55. PCT)微生物に対して非特異的付着性及び/又は凝集性を付与又は増強する方法及び遺伝子

    堀克敏

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    Applicant:東海国立大学機構 名古屋大学 名古屋工業大学

    Application no:PCT/JP2008/053591  Date applied:2008.2

    Announcement no:WO2009/104281  Date announced:2011.6

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  56. CN)微生物に対して非特異的付着性及び/又は凝集性を付与又は増強する方法及び遺伝子

    堀克敏

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    Applicant:東海国立大学機構 名古屋大学 名古屋工業大学

    Application no:CN200880128886  Date applied:2008.2

    Announcement no:CN102037126  Date announced:2011.4

    Patent/Registration no:CN102037126 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  57. US)微生物に対して非特異的付着性及び/又は凝集性を付与又は増強する方法及び遺伝子

    堀克敏

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    Applicant:東海国立大学機構 名古屋大学 名古屋工業大学

    Application no:US8399240  Date applied:2008.2

    Announcement no:US201104552  Date announced:2011.2

    Patent/Registration no:US8399240 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  58. KR)微生物に対して非特異的付着性及び/又は凝集性を付与又は増強する方法及び遺伝子

    堀克敏

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    Applicant:東海国立大学機構 名古屋大学 名古屋工業大学

    Application no:KR20107021175  Date applied:2008.2

    Announcement no:KR20100132506  Date announced:2010.12

    Patent/Registration no:KR101554767 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  59. EP)微生物に対して非特異的付着性及び/又は凝集性を付与又は増強する方法及び遺伝子

    堀克敏

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    Applicant:東海国立大学機構 名古屋大学 名古屋工業大学

    Application no:EP08721039  Date applied:2008.2

    Announcement no:EP2256206  Date announced:2010.12

    Patent/Registration no:EP2256206 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  60. JP)微生物に対して非特異的付着性及び/又は凝集性を付与又は増強する方法及び遺伝子

    堀克敏

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    Applicant:東海国立大学機構 名古屋大学 名古屋工業大学

    Application no:2009-554183  Date applied:2008.2

    Announcement no:WO2009/104281  Date announced:2009.8

    Patent/Registration no:5261775 

    Country of applicant:Foreign country   Country of acquisition:Foreign country

  61. 自己破砕微生物による有用物質の生産方法

    堀 克敏、海野 肇、丹治 保典

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    Applicant:名古屋大学 名古屋工業大学 東京工業大学長

    Application no:2002-016422  Date applied:2002.1

    Announcement no:2003-219895  Date announced:2003.8

    Patent/Registration no:03799402  Date registered:2006.5 

    Country of applicant:Domestic   Country of acquisition:Domestic

  62. ポリヒドロキシアルカン酸とラムノリピッドの同時生産法

    堀 克敏,海野 肇

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    Applicant:堀 克敏 財団法人 理工学振興会

    Application no:2001-245361  Date applied:2001.8

    Announcement no:2003-52368  Date announced:2003.2

    Country of applicant:Domestic  

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Teaching Experience (On-campus) 47

  1. 生命システム工学セミナー生命2A~2D

    2019

  2. 分子生命環境プロセス

    2019

  3. 生命システム工学セミナー1A~1D

    2019

  4. 生命システム工学基礎論

    2019

  5. 化学生命工学序論

    2019

  6. 化学生命工学演習

    2019

  7. 生物反応工学

    2019

  8. 生命システム工学セミナー2C

    2018

  9. 生命システム工学セミナー2B

    2018

  10. 生命システム工学セミナー2A

    2018

  11. 分子生命環境プロセス

    2018

  12. 生命システム工学セミナー1D

    2018

  13. 生命システム工学セミナー1C

    2018

  14. 生命システム工学セミナー1B

    2018

  15. 生命システム工学セミナー1A

    2018

  16. 生命システム基礎論

    2018

  17. 環境生物工学

    2018

  18. 生命システム工学セミナー2E

    2018

  19. 化学生命工学演習

    2018

  20. 生化学3及び演習

    2018

  21. 生命システム工学特別実験及び演習4

    2018

  22. 生命システム工学特別実験及び演習3

    2018

  23. 生命システム工学特別実験及び演習2

    2018

  24. 生命システム工学特別実験及び演習1

    2018

  25. 生命システム工学セミナー2D

    2018

  26. バイオマテリアルセミナー1A

    2016

  27. バイオマテリアルセミナー1B

    2016

  28. 環境生物工学特論

    2015

  29. 環境生物工学

    2015

  30. 生物機能工学特論Ⅰ

    2013

  31. 生物機能工学PBL

    2013

  32. 生物機能工学PBL

    2012

  33. 微生物学

    2012

  34. バイオマテリアル特別実験及び演習

    2012

  35. バイオマテリアルセミナー1C

    2012

  36. バイオマテリアルセミナー1B

    2012

  37. バイオマテリアルセミナー1A

    2012

  38. 化学工学基礎2

    2012

  39. バイオマテリアルセミナー1D

    2012

  40. バイオマテリアルセミナー1D

    2011

  41. バイオマテリアルセミナー1C

    2011

  42. バイオマテリアルセミナー1B

    2011

  43. バイオマテリアルセミナー1A

    2011

  44. バイオマテリアル基礎論

    2011

  45. 生物機能工学PBL

    2011

  46. 微生物学

    2011

  47. バイオマテリアル特別実験及び演習

    2011

▼display all

 

Social Contribution 21

  1. SDGsを推進する微生物・酵素技術の開発と社会実装

    Role(s):Lecturer, Informant, Organizing member, Demonstrator

    名古屋大学大学院工学研究科  テクノ・フェア2023  2023.10

  2. 高度なオリジナル微生物技術でバイオビジネスを拓く

    Role(s):Appearance, Lecturer

    ASFサイエンストーク  あいちサイエンス・コミュニケーション・ネットワーク  2023.9

  3. 人と微生物とSDGs

    Role(s):Lecturer

    Common-S. サカエ大学  名大カフェ  2022.3

  4. 除去とウイルス除去の科学

    Role(s):Lecturer

    愛知サイエンスフェスティバル  サイエンストーク  2021.11

  5. 河川の水をきれいに~「生物処理技術」とは~

    Role(s):Lecturer

    守山生涯学習センター  なごや環境大学共育講座  2021.10

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    Type:Lecture

    河川の水をきれいに~「生物処理技術」とは~

  6. マスクの科学

    Role(s):Lecturer

    愛知サイエンスフェスティバル  サイエンストーク  2020.10

  7. 微生物接着タンパク質AtaAの応用の拡がり(分子固定、表面機能化、分子輸送等)

    Role(s):Lecturer

    科学技術振興機構  JST新技術説明会  2020.9

  8. 河川の水をきれいに~「生物処理技術」とは~

    Role(s):Lecturer

    熱田生涯学習センター  2019.11

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    Audience: General

    Type:Lecture

  9. NHKカルチャー大河講座「ひとの大学」

    Role(s):Lecturer

    2017.3

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    Audience: High school students, College students, Graduate students, Teachers, Guardians, Researchesrs, General, Scientific, Company, Civic organization, Governmental agency, Media

    Type:Seminar, workshop

    微生物の力の可能性

  10. テクノ・フェア名大2016 未来志向

    Role(s):Lecturer

    2016.9

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    Type:Science festival

    何にでも強力にくっつく微生物、油分解微生物を使った革新的排水処理技術

  11. 何にでも強力にくっつく微生物

    Role(s):Lecturer

    イノベーション・ジャパン2016  2016.8

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    Type:Science festival

    何にでも強力にくっつく微生物

  12. 油脂分解微生物を使った革新的排水処理技術

    Role(s):Lecturer

    JSTフェア  2016.8

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    Type:Science festival

    油脂分解微生物を使った革新的排水処理技術

  13. 油分解微生物を使った革新的排水処理技術

    Role(s):Lecturer

    テクノ・フェア名大2015 名大発・技術移転の萌芽  2015.9

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    Type:Science festival

    油分解微生物を使った革新的排水処理技術

  14. バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

    Role(s):Lecturer

    FIRSTシンポジウム「科学技術が拓く2030年」へのシナリオ  2014.2 - 2014.3

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    Type:Seminar, workshop

    バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

  15. 知られざる微生物の世界

    Role(s):Lecturer

    愛知県立西尾高校出張講義  2013.12

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    Type:Visiting lecture

    知られざる微生物の世界

  16. バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

    Role(s):Lecturer

    INCHEM TOKYO 2013  2013.10

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    Type:Science festival

    バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

  17. バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

    Role(s):Lecturer

    テクノ・フェア名大2013 工学が挑む新時代の科学・技術  2013.9

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    Type:Science festival

    バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

  18. 微生物に非特異的付着性/凝集性を付与する遺伝子による工業用微生物の固定化技術

    Role(s):Lecturer

    国際医薬品原料・中間体展2013  2013.4

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    Type:Science festival

    微生物に非特異的付着性/凝集性を付与する遺伝子による工業用微生物の固定化技術

  19. バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

    Role(s):Lecturer

    テクノ・フェア名大2012 未来を明日に近づける技術  2012.8

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    Type:Science festival

    バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

  20. 身近な微生物の驚くべき能力とその利用を目指す微生物研究の最前線

    Role(s):Lecturer

    さかえサイエンストーク, 名古屋  2011.10

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    Type:Seminar, workshop

    身近な微生物の驚くべき能力とその利用を目指す微生物研究の最前線

  21. バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築

    Role(s):Lecturer

    テクノ・フェア2011 名大もの作り最前線-創造から技術へ-  2011.9

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    Type:Science festival

    展示と講演「バクテリオナノファイバー蛋白質の機能を基盤とする界面微生物プロセスの構築」

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Media Coverage 31

  1. 日本ゼオンなど、微生物の力でカーボンナノチューブを分解する手法を開発 Internet

    マイナビニュース  2023.12

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    Author:Other 

  2. 微生物 油分解し排水処理 Newspaper, magazine

    中日新聞  2023.7

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    Author:Other 

  3. 名古屋大学発ベンチャー核に微生物と酵素でSDGs加速目指し産学連携拡大 Newspaper, magazine

    科学新聞  2023.6

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    Author:Other 

  4. 『くらしの中から考える マスクどうする?』 Newspaper, magazine

    中日新聞  2022.9

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    Author:Other 

  5. 『ウイルス不活性化装置開発 名大発ベンチャーのフレンドマイクローブ 地元企業と連携 フィルター不要で除菌』 Newspaper, magazine

    中部経済新聞  2022.1

  6. 『マスクの効果 顔隙間で半減』 Newspaper, magazine

    中日新聞  2021.6