Faculty Profiles - MASUTANI, Chikahide

Papers - MASUTANI, Chikahide

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Novel mechanisms for the removal of strong replication-blocking HMCES- and thiazolidine-DNA adducts in humans. Reviewed

Sugimoto Y, Masuda Y, Iwai S, Miyake Y, Kanao R, Masutani C

Nucleic acids research 2023.4

RFWD3 and translesion DNA polymerases contribute to PCNA modification-dependent DNA damage tolerance Reviewed

Rie Kanao, Hidehiko Kawai, Toshiyasu Taniguchi, Minoru Takata, Chikahide Masutani

Life Science Alliance Vol. 5 ( 12 ) 2022.7

Regulation of HLTF-mediated PCNA polyubiquitination by RFC and PCNA monoubiquitination levels determines choice of damage tolerance pathway. Reviewed

Masuda Y, Mitsuyuki S, Kanao R, Hishiki A, Hashimoto H, Masutani C

Nucleic acids research 2018.10

USP7 is a suppressor of PCNA ubiquitination and oxidative-stress-induced mutagenesis in human cells. Reviewed

Kashiwaba, S., Kanao, R., Masuda, Y., Kusumoto-Matsuo, R., Hanaoka, F., Masutani, C.

Cell Reports Vol. 13 page： 2072-2080 2015.12

Different types of interaction between PCNA and PIP boxes contribute to distinct cellular functions of Y-family DNA polymerases Reviewed

Masuda Yuji, Kanao Rie, Kaji Kentaro, Ohmori Haruo, Hanaoka Fumio, Masutani Chikahide

NUCLEIC ACIDS RESEARCH Vol. 43 ( 16 ) page： 7898 - 7910 2015.9

Relevance of simultaneous mono-ubiquitinations of multiple units of PCNA homo-trimers in DNA damage tolerance. Reviewed International journal

Rie Kanao, Yuji Masuda, Saori Deguchi, Mayumi Yumoto-Sugimoto, Fumio Hanaoka, Chikahide Masutani

PloS one Vol. 10 ( 2 ) page： e0118775 2015.2

Authorship：Last author,　Corresponding author Language：Japanese Publishing type：Research paper (scientific journal)

DNA damage tolerance (DDT) pathways, including translesion synthesis (TLS) and additional unknown mechanisms, enable recovery from replication arrest at DNA lesions. DDT pathways are regulated by post-translational modifications of proliferating cell nuclear antigen (PCNA) at its K164 residue. In particular, mono-ubiquitination by the ubiquitin ligase RAD18 is crucial for Polη-mediated TLS. Although the importance of modifications of PCNA to DDT pathways is well known, the relevance of its homo-trimer form, in which three K164 residues are present in a single ring, remains to be elucidated. Here, we show that multiple units of a PCNA homo-trimer are simultaneously mono-ubiquitinated in vitro and in vivo. RAD18 catalyzed sequential mono-ubiquitinations of multiple units of a PCNA homo-trimer in a reconstituted system. Exogenous PCNA formed hetero-trimers with endogenous PCNA in WI38VA13 cell transformants. When K164R-mutated PCNA was expressed in these cells at levels that depleted endogenous PCNA homo-trimers, multiple modifications of PCNA complexes were reduced and the cells showed defects in DDT after UV irradiation. Notably, ectopic expression of mutant PCNA increased the UV sensitivities of Polη-proficient, Polη-deficient, and REV1-depleted cells, suggesting the disruption of a DDT pathway distinct from the Polη- and REV1-mediated pathways. These results suggest that simultaneous modifications of multiple units of a PCNA homo-trimer are required for a certain DDT pathway in human cells.

DOI： 10.1371/journal.pone.0118775

Web of Science

Scopus

PubMed

Mechanisms of accurate translesion synthesis by human DNA polymerase h. Reviewed

Masutani, C., Kusumoto, R., Iwai, S., and Hanaoka, F.

EMBO J. Vol. 19 page： 3100-3109 2000

Xeroderma pigmentosum variant (XP-V) correcting protein from HeLa cells has a thymine dimer bypass DNA polymerase activity. Reviewed

Masutani, C., Araki, M., Yamada, A., Kusumoto, R., Nogimori, T., Maekawa, T., Iwai, S., and Hanaoka, F.

EMBO J. Vol. 18 page： 3491-3501 1999

The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase h. Reviewed

Masutani, C., Kusumoto, R., Yamada, A., Dohmae, N., Yokoi, M., Yuasa, M., Araki, M., Iwai, S., Takio, K., and Hanaoka, F.

Nature Vol. 399 page： 700-704 1999

Acetaldehyde induces NER repairable mutagenic DNA lesions. Reviewed

Sonohara Y, Takatsuka R, Masutani C, Iwai S, Kuraoka I

Carcinogenesis 2021.9

Stepwise multipolyubiquitination of p53 by the E6AP-E6 ubiquitin ligase complex Reviewed

Masuda Yuji, Saeki Yasushi, Arai Naoko, Kawai Hidehiko, Kukimoto Iwao, Tanaka Keiji, Masutani Chikahide

JOURNAL OF BIOLOGICAL CHEMISTRY Vol. 294 ( 41 ) page： 14860 - 14875 2019.10

Spatiotemporal regulation of PCNA ubiquitination in damage tolerance pathways Reviewed

Masuda Yuji, Masutani Chikahide

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY Vol. 54 ( 5 ) page： 418 - 442 2019.9

Preferential digestion of PCNA-ubiquitin and p53-ubiquitin linkages by USP7 to remove polyubiquitin chains from substrates. Reviewed

Masuda Y, Kanao R, Kawai H, Kukimoto I, Masutani C

The Journal of biological chemistry 2019.1

Regulation of DNA damage tolerance in mammalian cells by post-translational modifications of PCNA Reviewed

Kanao Rie, Masutani Chikahide

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS Vol. 803 page： 82 - 88 2017.10

Biochemical analysis of HLTF, a human homologue of SWI/SNF-related ubiquitin ligase RAD5

Masuda Yuji, Kanao Rie, Masutani Chikahide

GENES & GENETIC SYSTEMS Vol. 91 ( 6 ) page： 366 - 366 2016.12

Stimulation of human DNA polymerase eta by PCNA

Masuda Yuji, Kanao Rie, Masutani Chikahide

GENES & GENETIC SYSTEMS Vol. 90 ( 6 ) page： 368 - 368 2015.12

The BAH domain of BAF180 is required for PCNA ubiquitination. Reviewed

Niimi A, Hopkins SR, Downs JA, Masutani C

Mutation Research Vol. 779 page： 16-23 2015.6

UV-induced mutations in epidermal cells of mice defective in DNA polymerase η and/or ι. Reviewed

Kanao R., Yokoi M., Ohkumo T., Sakurai Y., Dotsu K., Kura S., Nakatsu Y., Tsuzuki T., Masutani C., Hanaoka F.

DNA Repair (Amst) Vol. 29 page： 139-146 2015.5

UV-induced mutations in epidermal cells of mice defective in DNA polymerase eta and/or iota

Kanao Rie, Yokoi Masayuki, Ohkumo Tsuyoshi, Sakurai Yasutaka, Dotsu Kantaro, Kura Shinobu, Nakatsu Yoshimichi, Tsuzuki Teruhisa, Masutani Chikahide, Hanaoka Fumio

DNA REPAIR Vol. 29 page： 139 - 146 2015.5

Language：Japanese Publishing type：Research paper (scientific journal) Publisher：DNA Repair

Xeroderma pigmentosum variant (XP-V) is a human rare inherited recessive disease, predisposed to sunlight-induced skin cancer, which is caused by deficiency in DNA polymerase η (Polη). Polη catalyzes accurate translesion synthesis (TLS) past pyrimidine dimers, the most prominent UV-induced lesions. DNA polymerase ι (Polι) is a paralog of Polη that has been suggested to participate in TLS past UV-induced lesions, but its function in vivo remains uncertain. We have previously reported that Polη-deficient and Polη/Polι double-deficient mice showed increased susceptibility to UV-induced carcinogenesis. Here, we investigated UV-induced mutation frequencies and spectra in the epidermal cells of Polη- and/or Polι-deficient mice. While Polη-deficient mice showed significantly higher UV-induced mutation frequencies than wild-type mice, Polι deficiency did not influence the frequencies in the presence of Polη. Interestingly, the frequencies in Polη/Polι double-deficient mice were statistically lower than those in Polη-deficient mice, although they were still higher than those of wild-type mice. Sequence analysis revealed that most of the UV-induced mutations in Polη-deficient and Polη/Polι double-deficient mice were base substitutions at dipyrimidine sites. An increase in UV-induced mutations at both G:C and A:T pairs associated with Polη deficiency suggests that Polη contributes to accurate TLS past both thymine- and cytosine-containing dimers in vivo. A significant decrease in G:C to A:T transition in Polη/Polι double-deficient mice when compared with Polη-deficient mice suggests that Polι is involved in error-prone TLS past cytosine-containing dimers when Polη is inactivated.

DOI： 10.1016/j.dnarep.2015.02.006

Web of Science

Scopus

PubMed

Relevance of simultaneous mono-ubiquitnations of multiple units of PCNA homo-trimers in DNA damage tolerance. Reviewed

Kanao R, Masuda Y, Deguchi S, Yumoto-Sugimoto M, Hanaoka F, Masutani C

PLoS One Vol. 10 ( 2 ) page： e0118775 2015.2

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Papers - MASUTANI, Chikahide