ピッツバーグ大学癌研究所　日米がん派遣研究者

2003.6 - 2003.8

大阪大学大学院生命機能研究科　助手

2002.4 - 2005.6

大阪大学細胞生体工学センター　助手

1995.4 - 2002.3

理化学研究所　研究員

1994.4 - 1995.3

理化学研究所　基礎科学特別研究員

1991.4 - 1994.3

Relevance of simultaneous mono-ubiquitinations of multiple units of PCNA homo-trimers in DNA damage tolerance. Reviewed International journal

Rie Kanao, Yuji Masuda, Saori Deguchi, Mayumi Yumoto-Sugimoto, Fumio Hanaoka, Chikahide Masutani

PloS one   Vol. 10 ( 2 ) page： e0118775   2015.2

Mechanisms of accurate translesion synthesis by human DNA polymerase h. Reviewed

Masutani, C., Kusumoto, R., Iwai, S., and Hanaoka, F.

EMBO J.   Vol. 19   page： 3100-3109   2000

Xeroderma pigmentosum variant (XP-V) correcting protein from HeLa cells has a thymine dimer bypass DNA polymerase activity. Reviewed

Masutani, C., Araki, M., Yamada, A., Kusumoto, R., Nogimori, T., Maekawa, T., Iwai, S., and Hanaoka, F.

EMBO J.   Vol. 18   page： 3491-3501   1999

The XPV (xeroderma pigmentosum variant) gene encodes human DNA polymerase h. Reviewed

Masutani, C., Kusumoto, R., Yamada, A., Dohmae, N., Yokoi, M., Yuasa, M., Araki, M., Iwai, S., Takio, K., and Hanaoka, F.

Nature   Vol. 399   page： 700-704   1999

Acetaldehyde induces NER repairable mutagenic DNA lesions. Reviewed

Sonohara Y, Takatsuka R, Masutani C, Iwai S, Kuraoka I

Carcinogenesis     2021.9

Stepwise multipolyubiquitination of p53 by the E6AP-E6 ubiquitin ligase complex Reviewed

Masuda Yuji, Saeki Yasushi, Arai Naoko, Kawai Hidehiko, Kukimoto Iwao, Tanaka Keiji, Masutani Chikahide

JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 294 ( 41 ) page： 14860 - 14875   2019.10

Spatiotemporal regulation of PCNA ubiquitination in damage tolerance pathways Reviewed

Masuda Yuji, Masutani Chikahide

CRITICAL REVIEWS IN BIOCHEMISTRY AND MOLECULAR BIOLOGY   Vol. 54 ( 5 ) page： 418 - 442   2019.9

Preferential digestion of PCNA-ubiquitin and p53-ubiquitin linkages by USP7 to remove polyubiquitin chains from substrates. Reviewed

Masuda Y, Kanao R, Kawai H, Kukimoto I, Masutani C

The Journal of biological chemistry     2019.1

Regulation of DNA damage tolerance in mammalian cells by post-translational modifications of PCNA Reviewed

Kanao Rie, Masutani Chikahide

MUTATION RESEARCH-FUNDAMENTAL AND MOLECULAR MECHANISMS OF MUTAGENESIS   Vol. 803   page： 82 - 88   2017.10

Biochemical analysis of HLTF, a human homologue of SWI/SNF-related ubiquitin ligase RAD5

Masuda Yuji, Kanao Rie, Masutani Chikahide

GENES & GENETIC SYSTEMS   Vol. 91 ( 6 ) page： 366 - 366   2016.12

Stimulation of human DNA polymerase eta by PCNA

Masuda Yuji, Kanao Rie, Masutani Chikahide

GENES & GENETIC SYSTEMS   Vol. 90 ( 6 ) page： 368 - 368   2015.12

The BAH domain of BAF180 is required for PCNA ubiquitination. Reviewed

Niimi A, Hopkins SR, Downs JA, Masutani C

Mutation Research   Vol. 779   page： 16-23   2015.6

UV-induced mutations in epidermal cells of mice defective in DNA polymerase η and/or ι. Reviewed

Kanao R., Yokoi M., Ohkumo T., Sakurai Y., Dotsu K., Kura S., Nakatsu Y., Tsuzuki T., Masutani C., Hanaoka F.

DNA Repair (Amst)   Vol. 29   page： 139-146   2015.5

UV-induced mutations in epidermal cells of mice defective in DNA polymerase eta and/or iota

Kanao Rie, Yokoi Masayuki, Ohkumo Tsuyoshi, Sakurai Yasutaka, Dotsu Kantaro, Kura Shinobu, Nakatsu Yoshimichi, Tsuzuki Teruhisa, Masutani Chikahide, Hanaoka Fumio

DNA REPAIR   Vol. 29   page： 139 - 146   2015.5

Relevance of simultaneous mono-ubiquitnations of multiple units of PCNA homo-trimers in DNA damage tolerance. Reviewed

Kanao R, Masuda Y, Deguchi S, Yumoto-Sugimoto M, Hanaoka F, Masutani C

PLoS One   Vol. 10 ( 2 ) page： e0118775   2015.2

P-002 Interaction between PCNA and human DNA polymerase eta(Poster Sessions)

Masuda Yuji, Kanao Rie, Masutani Chikahide

  ( 44 )   2015

Analysis of PCNA interacting motifs of DNA polymerase

Masuda Yuji, Kanao Rie, Ohmori Haruo, Hanaoka Fumio, Masutani Chikahide

GENES & GENETIC SYSTEMS   Vol. 89 ( 6 ) page： 309 - 309   2014.12

Sphingosine, a Modulator of Human Translesion DNA Polymerase Activity

Kamath-Loeb Ashwini S., Balakrishna Sharath, Whittington Dale, Shen Jiang-Cheng, Emond Mary J., Okabe Takayoshi, Masutani Chikahide, Hanaoka Fumio, Nishimura Susumu, Loeb Lawrence A.

JOURNAL OF BIOLOGICAL CHEMISTRY   Vol. 289 ( 31 ) page： 21663 - 21672   2014.8

Sphingosine: A Modulator of Human Translesion DNA Polymerase Activity. Reviewed

Kamath-Loeb AS, Balakrishna S, Whittington D, Shen JC, Emond MJ, Okabe T, Masutani C, Hanaoka F, Nishimura S, Loeb LA.

Journal of Biological Chemistry     2014.7

Guanine- 5-carboxylcytosine base pairs mimic mismatches during DNA replication. Reviewed International journal

Toshihiro Shibutani, Shinsuke Ito, Mariko Toda, Rie Kanao, Leonard B Collins, Marika Shibata, Miho Urabe, Haruhiko Koseki, Yuji Masuda, James A Swenberg, Chikahide Masutani, Fumio Hanaoka, Shigenori Iwai, Isao Kuraoka

Scientific reports   Vol. 4   page： 5220 - 5220   2014.6

A cyclobutane thymine-N4-methylcytosine dimer is resistant to hydrolysis but strongly blocks DNA synthesis. Reviewed International journal

Junpei Yamamoto, Tomoko Oyama, Tomohiro Kunishi, Chikahide Masutani, Fumio Hanaoka, Shigenori Iwai

Nucleic acids research   Vol. 42 ( 3 ) page： 2075 - 2084   2014.2

Interactions between PCNA and DNA polymerases of Y-family

Masuda Yuji, Kanao Rie, Masutani Chikahide

GENES & GENETIC SYSTEMS   Vol. 88 ( 6 ) page： 360 - 360   2013.12

Novel mechanisms of PCNA ubiquitination

Masuda Yuji, Masutani Chikahide

GENES & GENETIC SYSTEMS   Vol. 87 ( 6 ) page： 387 - 387   2012.12

En bloc transfer of poly-ubiquitin chains to PCNA in vitro is mediated by two human E2-E3 pairs. Reviewed

*Masuda, Y., Suzuki, M., Kawai, H., Hishiki, A., Hashimoto, H., Masutani, C., Hishida, T., Suzuki, F., *Kamiya. K.

Nucl. Acids Res.   Vol. 40 ( 20 ) page： 10394-10407   2012.11

En bloc transfer of polyubiquitin chains to PCNA in vitro is mediated by two different human E2-E3 pairs

Masuda Yuji, Suzuki Miki, Kawai Hidehiko, Hishiki Asami, Hashimoto Hiroshi, Masutani Chikahide, Hishida Takashi, Suzuki Fumio, Kamiya Kenji

NUCLEIC ACIDS RESEARCH   Vol. 40 ( 20 ) page： 10394 - 10407   2012.11

Human DNA Polymerase η and Its Regulatory Mechanisms

Masutani Chikahide

Genes and environment : the official journal of the Japanese Environmental Mutagen Society   Vol. 34 ( 2 ) page： 63 - 69   2012.5

NBS1 Recruits RAD18 via a RAD6-like Domain and Regulates Pol eta-Dependent Translesion DNA Synthesis

Yanagihara Hiromi, Kobayashi Junya, Tateishi Satoshi, Kato Akihiro, Matsuura Shinya, Tauchi Hiroshi, Yamada Kouichi, Takezawa Jun, Sugasawa Kaoru, Masutani Chikahide, Hanaoka Fumio, Weemaes Corry M., Mori Toshio, Zou Lee, Komatsu Kenshi

MOLECULAR CELL   Vol. 43 ( 5 ) page： 788 - 797   2011.9

NBS1 recruits RAD18 via a RAD6-like domain and regulates Polh-dependent translesion DNA synthesis. Reviewed

Yanagihara, H., Kobayashi, J., Tateishi, S., Kato, A., Matsuura, S., Tauchi, H., Yamada, K., Takwzawa, J., Sugasawa, K., Masutani, C., Hanaoka, F., Weemaes, C.M., Mori, T., Zou, L., Komatsu. K.

Mol. Cell   Vol. 43 ( 5 ) page： 788-797   2011.9

Molecular chaperone Hsp90 regulates REV1-mediated mutagenesis Reviewed

Pozo, F.M., Oda, T., sekimoto, T., Murakumo, Y., Masutani, C., Hanaoka, F., Yamashita, T.

Mol. Cell. Biol.   Vol. 31 ( 16 ) page： 3396-3409   2011.8

Photosensitized [2+2] cycloaddition of N-acetylated cytosine affords stereoselective formation of cyclobutane pyrimidine dimer. Reviewed

Yamamoto, J., nishiguchi, K., Manabe, K., Masutani, C., Hanaoka, F., Iwai, S.

Nucl. Acids Res.   Vol. 39   page： 1165-1175   2011.2

Simultaneous disruption of two DNA polymerases, Polη and Polζ, in Avian DT40 cells unmasks the role of Polη in cellular response to various DNA lesions.

Hirota K, Sonoda E, Kawamoto T, Motegi A, Masutani C, Hanaoka F, Szüts D, Iwai S, Sale JE, Lehmann A, Takeda S

PLoS genetics   Vol. 6 ( 10 )   2010.10

Simultaneous disruption of two DNA polymerases, Polh and Polz, in Avian DT40 cells unmasks the role of Polh in cellular response to various DNA lesions. Reviewed

Hirota, K., Sonoda, E., Kawamoto, T., Motegi, A., Masutani, C., Hanaoka, F., Szuts, D., Iwai, S., Sale, J.E., Lehmann, A., Takeda, S.

PLoS Genet.   Vol. 6 ( 10 )   2010.10

Characterization of a Y-Family DNA Polymerase eta from the Eukaryotic Thermophile Alvinella pompejana.

Kashiwagi S, Kuraoka I, Fujiwara Y, Hitomi K, Cheng QJ, Fuss JO, Shin DS, Masutani C, Tainer JA, Hanaoka F, Iwai S

Journal of nucleic acids   Vol. 2010   2010.9

Characterization of a Y-family DNA polymerase h from the eukaryotic thermophile Alvinella pompejana. Reviewed

Kashiwagi, S., kuraoka, I., fujiwara, Y., Hitomi, K., Cheng, Q.J., Fuss, J.O., Shin, D.S., Masutani, C., Tainer, J.A., Hanaoka, F., Iwai, S.

J. Nucl. Acids   Vol. 2010   2010.9

Structure and mechanism of human DNA polymerase eta.

Biertümpfel C, Zhao Y, Kondo Y, Ramón-Maiques S, Gregory M, Lee JY, Masutani C, Lehmann AR, Hanaoka F, Yang W

Nature   Vol. 465 ( 7301 ) page： 1044 - 8   2010.6

Structure and mechanism of human DNA polymerase h. Reviewed

Biertümpfel, C., Zhao, Y., Kondo, Y., Ramón-Maiques, S., Gregory, M., Lee, J.Y., Masutani, C., Lehmann, A. R., *Hanaoka, F., and *Yang, W.

Nature   Vol. 465   page： 1044-1048   2010.6

Polymerization by DNA polymerase h is blocked by cis-diamminedichloroplatinum(II) 1,3-d(GpTpG) crosslink: Implications for cytotoxic effects in nucleotide excision repair-negative tumor cells. Reviewed

Chijiwa, S., Masutani, C., Hanaoka, F., Iwai, S., and *Kuraoka, I.

Carcinogenesis   Vol. 31   page： 388-393   2010.3

Polymerization by DNA polymerase eta is blocked by cis-diamminedichloroplatinum(II) 1,3-d(GpTpG) cross-link: implications for cytotoxic effects in nucleotide excision repair-negative tumor cells.

Chijiwa S, Masutani C, Hanaoka F, Iwai S, Kuraoka I

Carcinogenesis   Vol. 31 ( 3 ) page： 388 - 93   2010.3

The Molecular Chaperone Hsp90 Regulates Accumulation of DNA Polymerase eta at Replication Stalling Sites in UV-Irradiated Cells

Sekimoto Takayuki, Oda Tsukasa, Pozo Franklin Mayca, Murakumo Yoshiki, Masutani Chikahide, Hanaoka Fumio, Yamashita Takayuki

MOLECULAR CELL   Vol. 37 ( 1 ) page： 79 - 89   2010.1

Critical amino acids involved in erroneous incorporation of oxidized nucleotides by human DNA polymerase h and k. Reviewed

Katafuchi, A., Sassa, A., Niimi, N., Gruz, P., Fujimoto, H., Masutani, C., Hanaoka, F., Ohta, T., and *Nohmi, T.

Nucl. Acids Res.   Vol. 38   page： 859-867   2010.1

The molecular chaperone Hsp90 regulates accumulation of DNA polymerase h at replication stalling sites in UV-irradiated cells. Reviewed

Sekimoto, T., Oda, T., Pozo, F. M., Murakumo, Y., Masutani, C., Hanaoka, F., and *Yamashita, T.

Mol. Cell   Vol. 37   page： 79-89   2010.1

Critical amino acids in human DNA polymerases eta and kappa involved in erroneous incorporation of oxidized nucleotides.

Katafuchi A, Sassa A, Niimi N, Grúz P, Fujimoto H, Masutani C, Hanaoka F, Ohta T, Nohmi T

Nucleic acids research   Vol. 38 ( 3 ) page： 859 - 67   2010.1

A novel interaction between human DNA polymerase h and MutLa. Reviewed

Kanao, R., Hanaoka, F., and *Masutani, C.

Biochem. Biophys. Res. Commun.   Vol. 389   page： 40-45   2009.11

Translesional DNA synthesis through a C8-guanyl adduct of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP) in vitro: REV1 inserts dC opposite the lesion and DNA polymerase k potentially catalyzes extension reaction from the 3'-dC terminus. Reviewed

Fukuda, H., Takamura-Enya, T., Masuda, Y., Nohmi, T., Seki, C., Kamiya, K., Sugimura, T., Masutani, C., Hanaoka, F., and *Nakagama, H.

J. Biol. Chem.   Vol. 284   page： 25585-25592   2009.9

Interaction with DNA polymerase h is required for nuclear accumulation of REV1 and suppression of spontaneous mutations in human cells. Reviewed

Akagi, J., *Masutani, C., Kataoka, Y., Kan, T., Ohashi, E., Mori, T., Ohmori, H., and Hanaoka, F.

DNA Repair   Vol. 8   page： 585-599   2009.5

Penicilliols A and B, novel inhibitors specific to mammalian Y-family DNA polymerases. Reviewed

Kimura, T., Takeuchi, T., Kumamoto-Yonezawa, Y., Ohashi, E., Ohmori, H., Masutani, C., Hanaoka, F., Sugawara, F., Yoshida, H., and *Mizushina, Y.

Bioorg. Medicinal Chem.   Vol. 17   page： 1811-1816   2009.3

Specificity of mutations induced by incorporation of oxidized dNTPs into DNA by human DNA polymerase h. Reviewed

Hidaka, K., Yamada, M., Kamiya, H., Masutani, C., Harashima, H., Hanaoka, F., and *Nohmi, T.

DNA Repair   Vol. 7   page： 497-506   2008

Miscoding property of 2'-deoxyinosine, a nitric oxide-derived DNA adduct, during translesion synthesis catalyzed by human DNA polymerases. Reviewed

*Yasui, M., Suenaga, E., Koyama, N., Masutani, C., Hanaoka, F., Gruz, P., Shibutani, S., Nohmi, T., Hayashi, M., and Honma, M.

J. Mol. Biol.   Vol. 377   page： 1015-1023   2008

Efficient and erroneous incorporation of oxidized DNA precursors by human DNA polymerase h. Reviewed

Shimizu, M., Gruz, P., Kamiya, H., Masutani, C., Xu, Y., Sugiyama, H., Harashima, H., Hanaoka, F., and Nohmi, T.

Biochemistry   Vol. 46   page： 5515-5522   2007

A second PCNA loader complex, Ctf18-RFC, specifically stimulates DNA polymerase h activity. Reviewed

Shiomi, Y., Masutani, C., Hanaoka, F., Kimura, H., and *Tsurimoto, T.

J. Biol. Chem.   Vol. 282   page： 20906-20914   2007

2-Hydroxy-2'-deoxyadenosine 5'-triphosphate enhances A.T --> C.G mutations caused by 8-hydroxy-2'-deoxyguanosine 5'-triphosphate by suppressing its degradation upon replication in a HeLa extract. Reviewed

Satou, K., Kasai, H., Masutani, C., Hanaoka, F., Harashima, H., and Kamiya, H.

Biochemistry   Vol. 46   page： 6639-6646   2007

DNA polymerase h and q function in the same genetic pathway to generate mutations at A/T during somatic hypermutation of Ig genes. Reviewed

Masuda, K., Ouchida, R., Hikida, M., Kurosaki, T., Yokoi, M., Masutani, C., Seki, M., Wood, R.D., Hanaoka, F., and O-Wang, J.

J. Biol. Chem.   Vol. 282   page： 17387-17394   2007

Deficiency of the Caenorhabditis elegans DNA polymerase h homologue increases sensitivity to UV radiation during germ-line development. Reviewed

Ohkumo, T., Masutani, C., Eki, T., and Hanaoka, F.

Cell Struct. Funct.   Vol. 31   page： 29-37   2006

Ultraviolet B radiation induces epithelial tumors in mice lacking Polh and mesenchymal tumors in mice deficient for Poli. Reviewed

Ohkumo, T., Kondo, Y., Yokoi, M., Tsukamoto, T., Yamada, A., Sugimoto, T., Kanao, R., Higashi, Y., Kondoh, H., Tatematsu, M., Masutani, C., and Hanaoka, F.

Mol. Cell. Biol.   Vol. 26   page： 7696-7706   2006

A human DNA polymerase h complex containing Rad18, Rad6 and Rev1; proteomic analysis and targeting of the complex to the chromatin-bound fraction of cells undergoing replication fork arrest. Reviewed

Yuasa S., M., Masutani, C., Hirano, A., Cohn, M.A., Yamaizumi, M., Nakatani, Y., and Hanaoka, F.

Genes Cells   Vol. 11   page： 731-744   2006

Different mutationsignatures in DNA polymerase h- and MSH6-deficient mice suggest separate roles in antibody diversification. Reviewed

Martomo, S.A., Yang, W.W., Wersto, R.P., Ohkumo, T., Kondo, Y., Yokoi, M., Masutani, C., Hanaoka, F., and Gearhart, P.J.

Proc. Natl. Acad. Sci. USA   Vol. 102   page： 8656-8661   2005

Dual roles for DNA polymerase h in homologous DNA recombination and translesion DNA synthesis. Reviewed

Kawamoto, T., Araki, K., Sonoda, E., Yamashita, Y.M., Harada, K.K., Kikuchi, K., Masutani, C., Hanaoka, F., Nozaki, K., Hashimoto, N., and Takeda, S.

Mol. Cell   Vol. 20   page： 793-799   2005

Error-prone translesion synthesis by human DNA polymerase h on DNA containing deoxyadenosine adducts of 7.8-dihydroxy-9,10-epoxy-7,8,9,10-tetrahydrobenzo[a]pyrene. Reviewed

Chiapperino, D., Cai, M., Sayer, J.M., Yagi, H., Kroth, H., Masutani, C., Hanaoka, F., Jerina, D.M., and Cheh, A.M.

J. Biol. Chem.   Vol. 280   page： 39684-39692   2005

Centrin 2 stimulates nucleotide excision repair by interacting with xeroderma pigmentosum group C protein. Reviewed

Nishi, R., Okuda, Y., Watanabe, E., Mori, T., Iwai, S., Masutani, C., Sugasawa, K., and Hanaoka, F.

Mol. Cell. Biol.   Vol. 25   page： 5664-5674   2005

Preferential cis-syn thymine dimer bypass by DNA polymerase h occurs with biased fidelity. Reviewed

McCulloch, S.D., Kokoska R.J., Masutani, C., Iwai, S., Hanaoka, F., and Kunkel, T.A.

Nature   Vol. 428   page： 97-100   2004

DNA binding properties of human DNA polymerase h: Implication for polymerase switching. Reviewed

Kusumoto, R., Masutani, C., Simmyo, S., Iwai, S., and Hanaoka, F.

Genes Cells   Vol. 9   page： 1139-1150   2004

High-efficiency bypass of DNA damage by human DNA polymerase Q. Reviewed

Seki, M., Masutani, C., Yang, L.W., Schuffert, A., Iwai, S., Bahar, I., and Wood, R.D.

EMBO J.   Vol. 23   page： 4484-4494   2004

Interaction of hREV1 with three human Y-family DNA polymerases. Reviewed

Ohashi, E., Murakumo, Y., Kanjo, N., Akagi, J., Masutani, C., Hanaoka, F., and Ohmori, H.

Genes Cells   Vol. 9   page： 523-531   2004

Translesion synthesis past estrogen-derived DNA adducts by human DNA polymerases h and k. Reviewed

Suzuki, N., Itoh, S., Poon, K., Masutani, C., Hanaoka, F., Ohmori, H., Yoshizawa, I., and Shibutani, S.

Biochemistry   Vol. 43   page： 6304-6311   2004

Chemical synthesis and translesion replication of a cis-syn cyclobutane thymine-uracil dimer. Reviewed

Takasawa, K., Masutani, C., Hanaoka, F., and Iwai, S.

Nucl. Acids Res.   Vol. 32   page： 1738-1745   2004

Palm mutants in DNA polymerases a and h alter DNA replication fidelity and translesion activity. Reviewed

Niimi, A., Limsirichaikul, S., Yoshida, S., Iwai, S., Masutani, C., Hanaoka, F., Kool, E. T., Nishiyama, Y., and Suzuki, M.

Mol. Cell. Biol.   Vol. 24   page： 2734-2746   2004

Erroneous incorporation of oxidized DNA precursors by Y-family DNA polymerases. Reviewed

Shimizu, M., Gruz, P., Kamiya, H., Kim, S.-R., Pisani, F.M., Masutani, C., Kanke, Y., Harashima, H., Hanaoka, F., and Nohmi, T.

EMBO Reports   Vol. 4   page： 269-273   2003

129-derived strains of mice are deficient in DNA polymerase i and have normal immunoglobulin hypermutation. Reviewed

McDonald, J.P., Frank, E.G., Plosky, B.S., Rogozin, I.B., Masutani, C., Hanaoka, F., Woodgate, R., and Gearhart, P.J.

J. Exp. Med.   Vol. 198   page： 635-643   2003

Efficiency of extension of mismatched primer termini across from cisplatin and oxaliplatin adducts by human DNA polymerases b and h in vitro. Reviewed

Bassett, E., Vaisman, A., Havener, J. M., Masutani, C., Hanaoka, F., and Chaney, S.G.

Biochemistry   Vol. 42   page： 14197-14206   2003

Identification and characterization of an intermediate in the alkali degradation of (6-4) photoproduct-containing DNA. Reviewed

Higurashi, M., Ohtsuki, T., Inase, A., Kusumoto, R., Masutani, C., Hanaoka, F., and Iwai, S.

J. Biol. Chem.   Vol. 278   page： 51968-51973   2003

8-Hydroxyguanine in a mutational hotspot of c-Ha-ras gene causes misreplication, “action-at-a-distance" mutagenesis and inhibition of replication. Reviewed

Jaloszynski, P., Masutani, C., Hanaoka, F., Perwz, A.B., and Nishimura, S.

Nucl. Acids Res.   Vol. 31   page： 6085-6095   2003

Molecular analysis of mutations in DNA polymerase h in xeroderma pigmentosum-variant patients. Reviewed

Broughton, B.C., Cordonnier, A., Kleijer, W.J., Jaspers, N.G.J., Fawcett, H., Raams, A., Garritsen, V.H., Stary, A., Avril, M.-F., Boudsocq, F., Masutani, C., Hanaoka, F., Fuchs, R.P., Sarasn, A., and Lehmann, A.R.

Proc. Natl. Acad. Sci. USA   Vol. 99   page： 815-820   2002

Frameshifts and deletions during in vitro translesion synthesis past Pt-DNA adducts by DNA polymerase b and h. Reviewed

Bassett, E., Vaisman, A., Tropea, K.A., McCall, C.M., Masutani, C., Hanaoka, F., and Chaney, S.G.

DNA Repair   Vol. 1   page： 1003-1016   2002

Detection of reduced RNA synthesis in UV-irradiated Cockayne syndrome group B cells using an isolated nuclear system. Reviewed

Yamada, A., Masutani, C., and Hanaoka, F.

Biochim. Biophys. Acta   Vol. 1592   page： 129-134   2002

The carboxy-terminal domain of the XPC protein plays a crucial role in nucleotide excision repair through interactions with transcription factor IIH. Reviewed

Uchida, A., Sugasawa, K., Masutani, C., Dohmae, N., Araki, M., Yokoi, M., Ohkuma, Y., and Hanaoka, F.

DNA Repair   Vol. 1   page： 449-461   2002

Translesion synthesis by human DNA polymerase h across thymine glycol lesions. Reviewed

Kusumoto, R., Masutani, C., Iwai, S., and Hanaoka, F.

Biochemistry   Vol. 41   page： 6090-6099   2002

Preferential misincorporation of purine nucleotides by human DNA polymerase h opposite benzo[a]pyrene 7,8-diol 9,10-epoxide deoxyguanosine adducts. Reviewed

Chiapperino, D., Kroth, H., Kramarczuk, I. H., Sayer, J.M., Masutani, C., Hanaoka, F., Jerina, D.M., and Cheh, A.M.

J. Biol. Chem.   Vol. 277   page： 11765-11771   2002

Proofreading of DNA polymerase h-dependent replication errors. Reviewed

Bebenek, K., Matsuda, T., Masutani, C., Hanaoka, F., and Kunkel, T.A.

J. Biol. Chem.   Vol. 276   page： 2317-2320   2001

Oxygen free-radical damage to DNA: translesion synthesis by human DNA polymerase h and resistance to exonuclease action at cyclopurine deoxynucleoside residues. Reviewed

Kuraoka, I., Robins, P., Masutani, C., Hanaoka, F., Gasparutto, D., Cadet, J., Wood, R.D., and Lindahl, T.

J. Biol. Chem.   Vol. 276   page： 49283-49288   2001

Error rate and specificity of human and murin DNA polymerase h. Reviewed

Matsuda, T., Bebenek, K., Masutani, C., Rogozin, I.B., Hanaoka, F., and Kunkel, T.A.

J. Mol. Biol.   Vol. 312   page： 335-346   2001

Translesion DNA synthesis catalyzed by human pol h and pol k across 1,N6-ethenodeoxyadenosine. Reviewed

Levine, R.L., Miller, H., Grollman, A., Ohashi, E., Ohmori, H., Masutani, C., Hanaoka, F., and Moriya, M.

J. Biol. Chem.   Vol. 276   page： 18717-18721   2001

Centrosome protein centrin 2/caltractin 1 is part of the xeroderma pigmentosum group C complex that initiates global genome nucleotide excision repair. Reviewed

Araki, M., Masutani, C., Takemura, M., Uchida, A., Sugasawa, K., Kondo, J., Ohkuma, Y., and Hanaoka, F.

J. Biol. Chem.   Vol. 276   page： 18665-18672   2001

Mutagenic and non-mutagenic bypass of DNA lesions by Drosophila DNA polymerases dpolh and dpoli. Reviewed

Ishikawa, T., Uematsu, N., Mizukoshi, T., Iwai, S., Iwasaki, H., Masutani, C., Hanaoka, F., Ueda, R., Ohmori, H., and Todo, T.

J. Biol. Chem.   Vol. 276   page： 15155-15163   2001

ATP-dependent chromatin remodeling facilitates nucleotide excision repair of UV-induced DNA lesions in synthetic dinucleosomes. Reviewed

Ura, K., Araki, M., Saeki, H., Masutani, C., Ito, T., Iwai, S., Mizukoshi, T., Kaneda, Y., and Hanaoka, F.

EMBO J.   Vol. 20   page： 2004-2014   2001

Diversity of the damage recognition step in the global genomic nucleotide excision repair in vitro. Reviewed

Kusumoto, R., Masutani, C., Sugasawa, K., Iwai, S., Araki, M., Uchida, A., Mizukoshi, T., and Hanaoka, F.

Mutation Res.   Vol. 485   page： 219-227   2001

A multistep damage recognition mechanism for global genomic nucleotide excision repair. Reviewed

Sugasawa, K., Okamoto, T., Shimizu, Y., Masutani, C., Iwai, S., and Hanaoka, F.

Genes Dev.   Vol. 15   page： 507-521   2001

Genomic structure, chromosomal localization and identification of mutations in the xeroderma pigmentosum variant (XPV) gene. Reviewed

Yuasa, M., Masutani, C., Eki, T., and Hanaoka, F.

Oncogene   Vol. 19   page： 4721-4728   2000

3-methyladenine-DNA glycosylase (MPG protein) interacts with human RAD23 proteins. Reviewed

Miao, F., Bouziane, M., Dammann, R., Masutani, C., Hanaoka, F., Pfeifer, G., and O'Connor, T.R.

J. Biol. Chem.   Vol. 275   page： 28433-28438   2000

Error-prone bypass of certain DNA lesions by the human DNA polymerase h. Reviewed

Ohashi, E., Ogi, T., Kusumoto, R., Iwai, S., Masutani, C., Hanaoka, F., and Ohmori, H.

Genes Dev.   Vol. 14   page： 1589-1594   2000

Complementation of defective translesion synthesis and ultraviolet light sensitivity in xeroderma pigmentosum variant cells by human and mouse DNA polymerase h. Reviewed

Yamada, A., Masutani, C., Iwai, S., and Hanaoka, F.

Nucl. Acids Res.   Vol. 28   page： 2473-2480   2000

Modulation of TFIIH-associated kinase activity by complex formation and its relationship with CTD phosphorylation of RNA polymerase II. Reviewed

Watanabe, Y., Fujimoto, H., Watanabe, T., Maekawa, T., Masutani, C., Hanaoka, F., and Ohkuma, Y.

Genes Cells   Vol. 5   page： 407-423   2000

Low fidelity DNA synthesis by human DNA polymerase h. Reviewed

Matsuda, T., Bebenek, K., Masutani, C., Hanaoka, F., and Kunkel, T.A.

Nature   Vol. 404   page： 1011-1013   2000

Efficient translesion replication past oxaliplatin and cisplatin GpG adducts by human DNA polymerase eta. Reviewed

Vaisman, A., Masutani, C., Hanaoka, F., and Chaney, S.G.

Biochemistry   Vol. 39   page： 4575-4580   2000

The xeroderma pigmentosum group C protein complex XPC-HR23B plays an important role in the recruitment of TFIIH to damaged DNA. Reviewed

Yokoi, M., Masutani, C., Maekawa, T., Sugasawa, K., Ohkuma, Y., and Hanaoka, F.

J. Biol. Chem.   Vol. 275   page： 9870-9875   2000

Reconstitution of damage DNA excision reaction from SV40 minichromosomes with purified nucleotide excision repair proteins. Reviewed

Araki, M., Masutani, C., Maekawa, T., Watanabe, Y., Yamada, A., Kusumoto, R., Sakai, D., Sugasawa, K., Ohkuma, Y., and Hanaoka, F.

Mutation Res.   Vol. 459   page： 147-160   2000

Interaction of hHR23 with S5a: The ubiquitin-like domain of hHR23 mediates interaction with S5a subunit of 26S proteasome. Reviewed

Hiyama, H., Yokoi, M., Masutani, C., Sugasawa, K., Maekawa, T., Tanaka, K., Hoeijmakers, J.H.J., and Hanaoka, F.

J. Biol. Chem.   Vol. 274   page： 28019-28025   1999

Characterization of DNA recognition by the human UV-damaged-DNA binding protein. Reviewed

Fujiwara, Y., Masutani, C., Mizukoshi, T., Kondo, J., Hanaoka, F., and Iwai, S.

J. Biol. Chem.   Vol. 274   page： 20027-20033   1999

Benzimidazolium triflate-activated synthesis of (6-4) photoproduct-containing oligonucleotides and its application. Reviewed

Iwai, S., Mizukoshi, T., Fujiwara, Y., Masutani, C., Hanaoka, F., and Hayakawa, Y.

Nucl. Acids Res.   Vol. 27   page： 2299-2303   1999

A new Drosophila ultraviolet light-damaged DNA recognition endonuclease that selectively nicks a (6-4) photoproduct site. Reviewed

Kai, M., Todo, T., Wada, M., Ryo, H., Masutani, C., Kobayashi, H., Morioka, H., Ohtsuka, E., Hanaoka, F., and Sakaguchi, K.

Biochim. Biophys. Acta   Vol. 1397   page： 180-188   1998

Xeroderma pigmentosum group C protein complex is the initiator of global genome nucleotide excision repair. Reviewed

Sugasawa, K., Ng, J.M.Y., Masutani, C., Iwai, S., van der Spek, P.J., Eker, A.P.M., Hanaoka, F., Bootsma, D., and Hoeijmakers, J.H.J.

Mol. Cell   Vol. 2   page： 223-232   1998

Identification and characterization of XPC-binding domain of hHR23B. Reviewed

Masutani, C., Araki, M., Sugasawa, K., van der Spek, P.J., Yamada, A., Uchida, A., Maekawa, T., Bootsma, D., Hoeijmakers, J.H.J., and Hanaoka, F.

Mol. Cell. Biol.   Vol. 17   page： 6915-6923   1997

Two human homologues of Rad23 are functionally interchangeable in complex formation and stimulation of XPC repair activity. Reviewed

Sugasawa, K., Ng, J.M.Y., Masutani, C., Maekawa, T., Uchida, A., van der Spek, P.J., Eker, A., Rademakers, S., Visser, C., Aboussekhra, A., Wood, R.D., Hanaoka, F., Bootsma, D., and Hoeijmakers, J.H.J.

Mol. Cell. Biol.   Vol. 17   page： 6924-6931   1997

XPC and human homologs of RAD23: intracellular localization and relationship to other nucleotide excision repair complexes. Reviewed

van der Spek, P.J., Eker, A., Rademakers, S., Visser, C., Sugasawa, K., Masutani, C., Hanaoka, F., Bootsma, D., and Hoeijmakers, J.H.J.

Nucl. Acids Res.   Vol. 24   page： 2551-2559   1996

Sequential binding of DNA repair proteins RPA and ERCC1 to XPA in vitro. Reviewed

Saijo, M., Kuraoka, I., Masutani, C., Hanaoka, F., and Tanaka, K.

Nucl. Acids Res.   Vol. 24   page： 4719-4724   1996

c-jun stimulates origin-dependent DNA unwinding by polyomavirus large T antigen. Reviewed

Ito, K., Asano, M., Hughes, P., Kohzaki, H., Masutani, C., Hanaoka, F., Kerppola, T., Curran, T., Murakami, Y., and Ito, Y.

EMBO J.   Vol. 15   page： 5636-5646   1996

HHR23B, a human RAD23 homologue, stimulates XPC protein in nucleotide excision repair in vitro. Reviewed

Sugasawa, K., Masutani, C., Uchida, A., Maekawa, T., van der Spek, P.J., Bootsma, D., Hoeijmakers, J.H.J., and Hanaoka, F.

Mol. Cell. Biol.   Vol. 16   page： 4852-4861   1996

Comparison of incorporation and extension of nucleotides in vitro opposite 8-hydroxyguanine (7,8 dihydro-8-oxoguanine) in hot spots of the c-Ha-ras gene. Reviewed

Kamiya, H., M-Kamiya, N., Fujimuro, M., Kido, K., Inoue, H., Nishimura, S., Masutani, C., Hanaoka, F., and Ohtsuka, E.

Jpn. J. Cancer Res.   Vol. 86   page： 270-276   1995

Stimulation of DNA synthesis by mouse DNA helicase B in a DNA replication system containing eukaryotic replication origins. Reviewed

Matsumoto, K., Seki, M., Masutani, C., Tada, S., Enomoto, T., and Ishimi, Y.

Biochemistry   Vol. 34   page： 7913-7922   1995

8-Hydroxyadenine (7,8-dihydro-8-oxoadenine) induces misincorporation in in vitro DNA synthesis and mutations in NIH 3T3 cells. Reviewed

Kamiya, H., Miura, H., M-Kamiya, N., Ishikawa, H., Sakaguchi, T., Inoue, H., Sasaki, T., Masutani, C., Hanaoka, F., Nishimura, S., and Ohtsuka, E.

Nucl. Acids Res.   Vol. 23   page： 2893-2899   1995

DNA repair protein XPA binds replication protein A (RPA). Reviewed

Matsuda, T., Saijo, M., Kuraoka, I., Kobayashi, T., Nakatsu, Y., Nagai, A., Enjoji, T., Masutani, C., Sugasawa, K., Hanaoka, F., Yasui, A., and Tanaka, K.

J. Biol. Chem.   Vol. 270   page： 4152-4157   1995

Purification and cloning of a nucleotide excision repair complex involving the xeroderma pigmentosum group C protein and a human homolog of yeast RAD23. Reviewed

Masutani, C., Sugasawa, K., Yanagisawa, J., Sonoyama, T., Ui, M., Enomoto, T., Takio, K., Tanaka, K., van der Spek, P.J., Bootsma, D., Hoeijmakers, J.H.J., and Hanaoka, F.

EMBO J.   Vol. 13   page： 1831-1843   1994

Chromosomal localization of three repair genes: the xeroderma pigmentosum group C gene and two human homologs of yeast RAD23. Reviewed

van der Spek, P.J., Smit, E.M.E., Beverloo, H.B., Sugasawa, K., Masutani, C., Hanaoka, F., Hoeijmakers, J.H.J., and Hagemeijer, A.

Genomics   Vol. 23   page： 651-658   1994

Cell-free repair of UV-damaged simian virus 40 chromosomes in human cell extracts: I. Development of a cell-free system detecting excision repair of UV-irradiated SV40 chromosomes in human cell extracts. Reviewed

Sugasawa, K., Masutani, C., and Hanaoka, F.

J. Biol. Chem.   Vol. 268   page： 9098-9104   1993

Cyclobutane thymine dimers in a ras proto-oncogene hot spot activate the gene by point mutation. Reviewed

Kamiya, H., Murata, N., Murata, T., Iwai, S., Matsukage, A., Masutani, C., Hanaoka, F., and Ohtsuka, E.

Nucl. Acids Res.   Vol. 21   page： 2355-2361   1993

Cell-free repair of UV-damaged simian virus 40 chromosomes in human cell extracts: II. Defective DNA repair synthesis by xeroderma pigmentosum cell extracts. Reviewed

Masutani, C., Sugasawa, K., Asahina, H., Tanaka, K., and Hanaoka, F.

J. Biol. Chem.   Vol. 268   page： 9105-9109   1993

An abasic site analogue activates a c-Ha-ras gene by a point mutation at modified and adjacent positions. Reviewed

Kamiya, H., Suzuki, M., Komatsu, Y., Miura, H., Kikuchi, K., Sakaguchi, T., Murata, N., Masutani, C., Hanaoka, F., and Ohtsuka, E.

Nucl. Acids Res.   Vol. 20   page： 4409-4415   1992

In vitro replication study of modified bases in ras sequences. Reviewed

Kamiya, H., Sakaguchi, T., Murata, N., Fujimuro, M., Miura, H., Ishikawa, H., Shimizu, M., Inoue, H., Nishimura, S., Matsukage, A., Masutani, C., Hanaoka, F., and Ohtsuka, E.

Chem. Pharm. Bull.   Vol. 40   page： 2792-2795   1992

Mouse DNA primase plays the principal role in determination of permissiveness for polyomavirus DNA replication. Reviewed

Eki, T., Enomoto, T., Masutani, C., Miyajima, A., Takada, R., Murakami, Y., Ohno, T., Hanaoka, F., and Ui, M.

J. Virol.   Vol. 65   page： 4874-4881   1991

DNA primase stimulatory factor from mouse FM3A cells has an RNase H activity. Reviewed

Masutani, C., Enomoto, T., Suzuki, M., Hanaoka, F., and Ui, M.

J. Biol. Chem.   Vol. 265   page： 10210-10216   1990

DNA primase-DNA polymerase eta assembly from mouse FM3A cells: purification of constituting enzymes, reconstitution, and analysis of RNA priming as coupled to DNA synthesis. Reviewed

Suzuki, M., Enomoto, T., Masutani, C., Hanaoka, F., Yamada, M., and Ui, M.

J. Biol. Chem.   Vol. 264   page： 10065-10071   1989

Human DNA polymerase eta and its regulatory mechanisms.

Masutani, C.（ Role： Sole author）

Genes and Environment  2012 

動物細胞の紫外線DNA損傷の修復と複製

益谷央豪（ Role： Sole author）

蛋白質核酸酵素2009年3月号増刊「染色体サイクル」  2009 

Xeroderma Pigmentosum Variant, XP-V: its product and biological roles.

Masutani, C., Hanaoka, F., and *Ahmad, S.I.（ Role： Joint author）

Adv. Exp. Med. Biol.  2008 

Use of RNAi in C. elegans.

Ohkumo, T., Masutani, C., Eki, T., and *Hanaoka, F.（ Role： Joint author）

Methods Mol. Biol.  2008 

真核細胞のＤＮＡ合成酵素pp219-226、プライマーゼ―真核細胞pp260-263

益谷央豪、花岡文雄（ Role： Joint author）

生物薬科学実験講座８遺伝子ＩＩ（名取俊二/中西義信・編集　廣川書店）  2003 

真核生物が獲得した天然の日焼け止めクリーム―XP-V群色素性乾皮症遺伝子産物；DNAポリメラーゼh（イータ）について-

大雲剛志、益谷央豪、花岡文雄（ Role： Joint author）

放射線生物研究  2002 

DNAポリメラーゼh：DNA修復欠損遺伝病の研究から損傷乗り越え型DNAポリメラーゼの発見へ

花岡文雄、益谷央豪（ Role： Joint author）

生化学  2002 

色素性乾皮症バリアントにおける損傷乗り越えDNA複製の異常

益谷央豪、花岡文雄（ Role： Joint author）

蛋白質核酸酵素（共立出版）増刊『DNA修復ネットワークとその破綻の分子病態』  2001 

Xeroderma pigmentosum variant: From a human genetic disorder to a novel DNA polymerase.

Masutani, C., Kusumoto, R., Yamada, A., Yuasa, M., Araki, M., Nogimori, T., Yokoi, M., Eki, T., Iwai, S., and Hanaoka, F.（ Role： Joint author）

Cold Spring Harbor Symp. Quantit. Biol.  2000 

バリアント型色素性乾皮症と損傷乗り越えDNA複製

益谷央豪、花岡文雄（ Role： Joint author）

医学のあゆみ（医歯薬出版）  2000 

V群色素性乾皮症遺伝子産物　DNAポリメラーゼh（イータ）について

楠本理加、益谷央豪、花岡文雄（ Role： Joint author）

ファルマシア  2000 

損傷を乗り越える新規DNAポリメラーゼ

山田亜夕美、益谷央豪、花岡文雄（ Role： Joint author）

生化学  2000 

色素性乾皮症バリアント群の原因遺伝子のクローニング遺伝子医学

湯浅真弓、益谷央豪、花岡文雄（ Role： Joint author）

メディカルドゥ  2000 

ヌクレオチド除去修復の分子機構

荒木真理人、益谷央豪、花岡文雄（ Role： Joint author）

蛋白質核酸酵素（共立出版）  1999 

色素性乾皮症研究の最前線-DNA修復欠損と損傷DNA複製欠損

益谷央豪、花岡文雄（ Role： Joint author）

実験医学（羊土社）  1999 

色素性乾皮症バリアント(XPV)遺伝子はDNAポリメラーゼhをコードする

益谷央豪、花岡文雄（ Role： Joint author）

実験医学（羊土社）  1999 

真核細胞のヌクレオチド除去修復

益谷央豪、花岡文雄（ Role： Joint author）

実験医学（羊土社）  1998 

DNA修復と細胞周期制御

横井雅幸、益谷央豪、花岡文雄（ Role： Joint author）

蛋白質核酸酵素（共立出版）  1996 

染色体複製の機構

益谷央豪、花岡文雄（ Role： Joint author）

細胞増殖の制御（豊島久真男編集）南江堂（東京）第２刷  1995 

真核細胞のヌクレオチド除去修復

益谷央豪、菅澤薫、花岡文雄（ Role： Joint author）

細胞工学（秀潤社）  1994 

無細胞系を用いたクロマチンDNAの複製と修復

菅澤薫、益谷央豪、花岡文雄（ Role： Joint author）

実験医学（羊土社）  1993 

Biochemical study on the molecular mechanisms of induced mutagenesis and post-replication repair pathways

Grant number：24310040  2012.4 - 2015.3

Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

Masuda Yuji, MASUTANI Chikahide, Kanao Rie

Crosstalk of translesion synthesis and checkpoint mechanisms

Grant number：24651045  2012.4 - 2014.3

Grants-in-Aid for Scientific Research  Grant-in-Aid for Challenging Exploratory Research

MASUTANI Chikahide, KANAO Rie

Generality of translesion synthesis coupling replication and repair

Grant number：22131008  2010.4 - 2015.3

Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)

HANAOKA FUMIO, MASUTANI Chikahide, YOKOI Masayuki

タンパク質のマルチ翻訳後修飾による損傷乗り越えＤＮＡ複製の制御

2010.4 - 2012.3

科学研究費補助金  基盤研究(B)

損傷乗り越え複製後のDNA損傷の修復と娘細胞への分配

Grant number：21651020  2009 - 2010

科学研究費助成事業  挑戦的萌芽研究

益谷 央豪