Faculty Profiles - KINOSHITA, Makoto

Papers - KINOSHITA, Makoto

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Identification of a Xenopus glutamine synthetase gene abundantly expressed in the embryonic nervous system but not in adult brain Reviewed Open Access

Seigo Hatada, Makoto Kinoshita, Makoto Noda, Makoto Asashima

FEBS Letters Vol. 371 ( 3 ) page： 287 - 292 1995.9

Language：English Publishing type：Research paper (scientific journal) Publisher：Elsevier {BV}

We used a PCR-based subtraction cloning procedure with concanavalin A-treated and -untreated animal caps from stage 9 Xenopus embryos to search for genes up-regulated during early neural development. One such gene was found to encode a protein homologous to several known glutamine synthetases, and we named it xGS, Molecular hybridization studies revealed that xGS mRNA is maternally transmitted and abundantly expressed in neuroectoderm-derived tissues during the gastrula and neurula stages, The expression of xGS mRNA in the nervous system continues until the larval stages, but declines thereafter and becomes undetectable in adult brain, Considering its metabolic activity and potential neuroprotective effect against the neurotoxic substances such as glutamate and ammonia, the glutamine synthetase may play an important role in the early stages of vertebrate neural development.

DOI： 10.1016/0014-5793(95)00913-t

Web of Science

HMG-X, a Xenopus gene encoding an HMG1 homolog, is abundantly expressed in the developing nervous system Reviewed Open Access

FEBS Letters Vol. 352 ( 2 ) page： 191 - 196 1994.9

Induction of apoptosis by the mouse Nedd2 gene, which encodes a protein similar to the product of the Caenorhabditis elegans cell death gene ced-3 and the mammalian IL-1 beta-converting enzyme Reviewed International coauthorship Open Access

S Kumar, M Kinoshita, M Noda, N G Copeland, N A Jenkins

Genes & Development Vol. 8 ( 14 ) page： 1613 - 1626 1994.7

Language：English Publishing type：Research paper (scientific journal) Publisher：COLD SPRING HARBOR LAB PRESS

By subtraction cloning we previously identified a set of mouse genes (named Nedd1 through Nedd10) with developmentally down-regulated expression in brain. We now show that one such gene, Nedd2, encodes a protein similar to the mammalian interleukin-1 beta-converting enzyme (ICE) and the product of the Caenorhabditis elegans cell death gene ced-3 (CED-3). Both ICE and CED-3 are known to encode putative cysteine proteases and induce apoptosis when overexpressed in cultured cells. Overexpression of Nedd2 in cultured fibroblast and neuroblastoma cells also resulted in cell death by apoptosis, which was suppressed by the expression of the human bcl-2 gene, indicating that Nedd2 is functionally similar to the ced-3 gene in C. elegans. We also show that during embryonic development, Nedd2 is highly expressed in several types of mouse tissue undergoing high rates of programmed cell death such as central nervous system and kidney. Our data suggest that Nedd2 is an important component of the mammalian programmed cell death machinery.

DOI： 10.1101/gad.8.14.1613

Vector Analysis of Corneal Astigmatism after Scleral Buckling Surgery Reviewed

Makoto Kinoshita, Hidenobu Tanihara, Akira Negi, Shin-Ichiro Kawano, Hitoshi Ishigouoka, Yoshiki Veda, Satomi Suzuki-Yoshida, Yoshihito Honda

Ophthalmologica Vol. 208 ( 5 ) page： 250 - 253 1994

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal) Publisher：S. Karger {AG}

We conducted a short-term prospective study of 125 eyes with retinal detachment to examine changes of corneal astigmatism about 2 weeks after scleral buckling surgery, using a vector method on 2-fold-angle rectangular coordinates. Segmental buckles of one to less than two quadrants produced significantly greater changes in astigmatism (1.65+/-0.97 dptr) than those of less than one quadrant (0.88+/-0.75 dptr) and those spanning two quadrants or more (1.09+/-0.38 dptr) (p=0.0005, Kruskal-wallis test; n=73). The amplitude of differential vectors after explant buckling surgery (1.33+/-0.89 dptr, n=24) was significantly greater than after implant buckling surgery (0.65+/-0.45 dptr, n=15) (p=0.009, Mann-Whitney U-test) with buckles of one quadrant or less. Differential vectors tended to direct toward the buckles. There was no obvious directional tendency in the case of encircling procedures (n=52).

DOI： 10.1159/000310501

Web of Science

癌遺伝子と神経系の発生・分化・機能 Invited Reviewed

木下　専、野田　亮

実験医学増刊「脳神経系の発生・分化と可塑性」 Vol. 11 ( 10 ) page： 104 - 112 1993.6

Expression of DRG during murine embryonic development Reviewed International coauthorship

Takashi Sazuka, Makoto Kinoshita, Yasuhiro Tomooka, Yoji Ikawa, Makoto Noda, Sharad Kumar

Biochemical and Biophysical Research Communications Vol. 189 ( 1 ) page： 371 - 377 1992.11

Central serous chorioretinopathy Open Access

1991

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Papers - KINOSHITA, Makoto