Faculty Profiles - GOSHIMA, Gohta

Papers - GOSHIMA, Gohta

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Identification of a TPX2-like microtubule-associated protein in Drosophila. Reviewed

Goshima G.

PLoS One Vol. 6 page： e28120 2011.11

EB1 promotes microtubule dynamics by recruiting Sentin in Drosophila cells. Reviewed

Li W, Miki T, Watanabe T, Kakeno M, Sugiyama I, Kaibuchi K, Goshima G.

J Cell Biol. Vol. 193 ( 6 ) page： 973-983 2011.6

Control of mitotic spindle length. Invited Reviewed

Goshima G, Scholey JM.

Annu Rev Cell Dev Biol Vol. 26 page： 21-57 2010.11

Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase. Reviewed

Uehara R, Goshima G.

Journal of Cell Biology Vol. 191 page： 259-267 2010.10

Determinants of myosin II cortical localization during cytokinesis. Reviewed

Uehara R, Goshima G, Mabuchi I, Vale RD, Spudich JA, Griffis ER.

Current Biology Vol. 22 page： 1080-1085 2010.6

*New look inside the spindle: microtubule-dependent microtuble generation within the spindle. Invited Reviewed

Goshima G, Kimura A

Current Opinion in Cell Biology Vol. Epub 2010.2

*The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Reviewed

Uehara R, Nozawa RS, Tomioka A, Petry S, Vale RD, Obuse C, Goshima G.

Proc Natl Acad Sci U S A. Vol. 106 page： 6998-7003 2009.4

*Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle Reviewed

Gohta Goshima, Mirjam Mayer, Nan Zhang, Nico Stuurman, Ronald D. Vale

Journal of Cell Biology Vol. 181 page： 421-429 2008.5

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

Since the discovery of gamma-tubulin, attention has focused on its involvement as a microtubule nucleator at the centrosome. However, mislocalization of gamma-tubulin away from the centrosome does not inhibit mitotic spindle formation in Drosophila melanogaster, suggesting that a critical function for gamma-tubulin might reside elsewhere. A previous RNA interference (RNAi) screen identified five genes (Dgt2-6) required for localizing gamma-tubulin to spindle microtubules. We show that the Dgt proteins interact, forming a stable complex. We find that spindle microtubule generation is substantially reduced after knockdown of each Dgt protein by RNAi. Thus, the Dgt complex that we name "augmin" functions to increase microtubule number. Reduced spindle microtubule generation after augmin RNAi, particularly in the absence of functional centrosomes, has dramatic consequences on mitotic spindle formation and function, leading to reduced kinetochore fiber formation, chromosome misalignment, and spindle bipolarity defects. We also identify a functional human homologue of Dgt6. Our results suggest that an important mitotic function for gamma-tubulin may lie within the spindle, where augmin and gamma-tubulin function cooperatively to amplify the number of microtubules.

Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Reviewed

Guo Y, Walther TC, Rao M, Stuurman N, Goshima G, Terayama K, Wong JS, Vale RD, Walter P, Farese RV.

Nature Vol. 453 ( 7195 ) page： 657-661 2008.5

Language：English Publishing type：Research paper (scientific journal)

Eukaryotic cells store neutral lipids in cytoplasmic lipid droplets enclosed in a monolayer of phospholipids and associated proteins. These dynamic organelles serve as the principal reservoirs for storing cellular energy and for the building blocks for membrane lipids. Excessive lipid accumulation in cells is a central feature of obesity, diabetes and atherosclerosis, yet remarkably little is known about lipid-droplet cell biology. Here we show, by means of a genome-wide RNA interference (RNAi) screen in Drosophila S2 cells that about 1.5% of all genes function in lipid-droplet formation and regulation. The phenotypes of the gene knockdowns sorted into five distinct phenotypic classes. Genes encoding enzymes of phospholipid biosynthesis proved to be determinants of lipid-droplet size and number, suggesting that the phospholipid composition of the monolayer profoundly affects droplet morphology and lipid utilization. A subset of the Arf1-COPI vesicular transport proteins also regulated droplet morphology and lipid utilization, thereby identifying a previously unrecognized function for this machinery. These phenotypes are conserved in mammalian cells, suggesting that insights from these studies are likely to be central to our understanding of human diseases involving excessive lipid storage.

*Genes required for mitotic spindle assembly in Drosophila S2 cells Reviewed

Gohta Goshima, Roy Wollman, Sarah S. Goodwin, Nan Zhang, Jonathan M. Scholey, Ronald D. Vale and Nico Stuurman.

Science Vol. 316 ( 5823 ) page： 417-421 2007.7

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.

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Papers - GOSHIMA, Gohta