Faculty Profiles - GOSHIMA, Gohta

Molines, AT; Lemière, J; Gazzola, M; Steinmark, IE; Edrington, CH; Hsu, CT; Real-Calderon, P; Suhling, K; Goshima, G; Holt, LJ; Thery, M; Brouhard, GJ; Chang, F

DEVELOPMENTAL CELL Vol. 57 ( 4 ) page： 466 - + 2022.2

Growth and division mode plasticity is dependent on cell density in marine-derived black yeasts Reviewed Open Access

GENES TO CELLS Vol. 27 ( 2 ) page： 124 - 137 2022.2

Fifteen compelling open questions in plant cell biology Reviewed Open Access

Roeder Adrienne H. K., Otegui Marisa S., Dixit Ram, Anderson Charles T., Faulkner Christine, Zhang Yan, Harrison Maria J., Kirchhelle Charlotte, Goshima Gohta, Coate Jeremy E., Doyle Jeff J., Hamant Olivier, Sugimoto Keiko, Dolan Liam, Meyer Heather, Ehrhardt David W., Boudaoud Arezki, Messina Carlos

PLANT CELL Vol. 34 ( 1 ) page： 72 - 102 2022.1

Cell tip growth underlies injury response of marine macroalgae Invited Reviewed Open Access

Shirae-Kurabayashi M, Edzuka T, Suzuki M, Goshima G.

PLoS ONE Vol. 17 ( 3 ) page： e0264827 2022

SS Microtubule-associated proteins promote microtubule generation in the absence of gamma-tubulin in human colon cancer cells Reviewed Open Access

Tsuchiya Kenta, Goshima Gohta

JOURNAL OF CELL BIOLOGY Vol. 220 ( 12 ) 2021.12

The 3D architecture and molecular foundations of de novo centriole assembly via bicentrioles Reviewed Open Access

Pereira Sonia Gomes, Sousa Ana Laura, Nabais Catarina, Paixao Tiago, Holmes Alexander J., Schorb Martin, Goshima Gohta, Tranfield Erin M., Becker Jorg D., Bettencourt-Dias Monica

CURRENT BIOLOGY Vol. 31 ( 19 ) page： 4340 - + 2021.10

Plant stem cell research is uncovering the secrets of longevity and persistent growth Reviewed Open Access

Umeda Masaaki, Ikeuchi Momoko, Ishikawa Masaki, Ito Toshiro, Nishihama Ryuichi, Kyozuka Junko, Torii Keiko U., Satake Akiko, Goshima Gohta, Sakakibara Hitoshi

PLANT JOURNAL Vol. 106 ( 2 ) page： 326 - 335 2021.4

Ran-GTP Is Non-essential to Activate NuMA for Mitotic Spindle-Pole Focusing but Dynamically Polarizes HURP Near Chromosomes Reviewed International journal Open Access

Tsuchiya, K; Hayashi, H; Nishina, M; Okumura, M; Sato, Y; Kanemaki, MT; Goshima, G; Kiyomitsu, T

CURRENT BIOLOGY Vol. 31 ( 1 ) page： 115 - + 2021.1

Rho of Plants GTPases and Cytoskeletal Elements Control Nuclear Positioning and Asymmetric Cell Division during Physcomitrella patens Branching Reviewed International journal Open Access

Yi, PS; Goshima, G

CURRENT BIOLOGY Vol. 30 ( 14 ) page： 2860 - + 2020.7

Transient cotransformation of CRISPR/Cas9 and oligonucleotide templates enables efficient editing of target loci in Physcomitrella patens Reviewed International journal Open Access

Yi, PS; Goshima, G

PLANT BIOTECHNOLOGY JOURNAL Vol. 18 ( 3 ) page： 599 - 601 2020.3

Kinesin-13 and Kinesin-8 Function during Cell Growth and Division in the Moss Physcomitrella patens[OPEN] Reviewed International journal Open Access

Leong, SY; Edzuka, T; Goshima, G; Yamada, M

PLANT CELL Vol. 32 ( 3 ) page： 683 - 702 2020.3

A versatile microfluidic device for highly inclined thin illumination microscopy in the moss Physcomitrella patens Reviewed International journal Open Access

Kozgunova, E; Goshima, G

SCIENTIFIC REPORTS Vol. 9 2019.10

Editorial overview: Cell division - from molecules to tissues Invited International coauthorship International journal

Goshima, G; Bellaïche, Y

CURRENT OPINION IN CELL BIOLOGY Vol. 60 page： III - V 2019.10

Kinetochore protein depletion underlies cytokinesis failure and somatic polyploidization in the moss Physcomitrella patens Reviewed International journal Open Access

Kozgunova, E; Nishina, M; Goshima, G

ELIFE Vol. 8 2019.3

Drosophila kinesin-8 stabilizes the kinetochore-microtubule interaction Reviewed International journal Open Access

Edzuka, T; Goshima, G

JOURNAL OF CELL BIOLOGY Vol. 218 ( 2 ) page： 474 - 488 2019.2

Moss Kinesin-14 KCBP Accelerates Chromatid Motility in Anaphase Reviewed International journal Open Access

Yoshida, MW; Yamada, M; Goshima, G

CELL STRUCTURE AND FUNCTION Vol. 44 ( 2 ) page： 95 - 104 2019

Identification of 15 New Bypassable Essential Genes of Fission Yeast Reviewed International coauthorship International journal Open Access

Takeda, A; Saitoh, S; Ohkura, H; Sawin, KE; Goshima, G

CELL STRUCTURE AND FUNCTION Vol. 44 ( 2 ) page： 113 - 119 2019

Microtubule nucleation and organization without centrosomes Invited Reviewed International journal Open Access

Yi, PS; Goshima, G

CURRENT OPINION IN PLANT BIOLOGY Vol. 46 page： 1 - 7 2018.12

The KCH Kinesin Drives Nuclear Transport and Cytoskeletal Coalescence to Promote Tip Cell Growth in Physcomitrella patens Open Access

Yamada Moe, Goshima Gohta

PLANT CELL Vol. 30 ( 7 ) page： 1496 - 1510 2018.7

SPIRAL2 Stabilises Endoplasmic Microtubule Minus Ends in the Moss Physcomitrella patens Reviewed International journal Open Access

Leong, SY; Yamada, M; Yanagisawa, N; Goshima, G

CELL STRUCTURE AND FUNCTION Vol. 43 ( 1 ) page： 53 - 60 2018

Human microcephaly ASPM protein is a spindle pole-focusing factor that functions redundantly with CDK5RAP2 Open Access

Tungadi Elsa A., Ito Ami, Kiyomitsu Tomomi, Goshima Gohta

JOURNAL OF CELL SCIENCE Vol. 130 ( 21 ) page： 3676 - 3684 2017.11

Cytoplasmic MTOCs control spindle orientation for asymmetric cell division in plants Open Access

Kosetsu Ken, Murata Takashi, Yamadaa Moe, Nishina Momoko, Boruc Joanna, Hasebe Mitsuyasu, Van Damme Daniel, Goshima Gohta

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA Vol. 114 ( 42 ) page： E8847 - E8854 2017.10

14-3-3 regulation of Ncd reveals a new mechanism for targeting proteins to the spindle in oocytes Open Access

Beaven Robin, Bastos Ricardo Nunes, Spanos Christos, Rome Pierre, Cullen C. Fiona, Rappsilber Juri, Giet Regis, Goshima Gohta, Ohkura Hiroyuki

JOURNAL OF CELL BIOLOGY Vol. 216 ( 10 ) page： 3029 - 3039 2017.10

Multiple kinesin-14 family members drive microtubule minus end-directed transport in plant cells Open Access

Yamada Moe, Tanaka-Takiguchi Yohko, Hayashi Masahito, Nishina Momoko, Goshima Gohta

JOURNAL OF CELL BIOLOGY Vol. 216 ( 6 ) page： 1705 - 1714 2017.6

Mitotic Spindle Assembly in Land Plants: Molecules and Mechanisms Invited Reviewed International journal Open Access

Yamada, M; Goshima, G

BIOLOGY-BASEL Vol. 6 ( 1 ) 2017.3

Shortening of Microtubule Overlap Regions Defines Membrane Delivery Sites during Plant Cytokinesis Open Access

de Keijzer Jeroen, Kieft Henk, Ketelaar Tijs, Goshima Gohta, Janson Marcel E.

CURRENT BIOLOGY Vol. 27 ( 4 ) page： 514 - 520 2017.2

Intra-spindle Microtubule Assembly Regulates Clustering of Microtubule-Organizing Centers during Early Mouse Development Reviewed Open Access

Watanabe S, Shioi G, Furuta Y, Goshima G.

Cell Rep Vol. 15 page： 54-60 2016.4

Augmin shapes the anaphase spindle for efficient cytokinetic furrow ingression and abscission Reviewed Open Access

Uehara R, Kamasaki T, Hiruma S, Poser I, Yoda K, Yajima J, Gerlich DW, Goshima G.

Mol Biol Cell Vol. 27 page： 812-27 2016.3

Live Cell Microscopy-Based RNAi Screening in the Moss Physcomitrella patens Invited

Miki T, Nakaoka Y, Goshima G.

Methods Mol Biol Vol. 1470 page： 225-46 2016

Imaging Mitosis in the Moss Physcomitrella patens Invited

Yamada M, Miki T, Goshima G.

Methods Mol Biol Vol. 1413 page： 263-82 2016

Five factors can reconstitute all three phases of microtubule polymerization dynamics Reviewed Open Access

Moriwaki T, Goshima G

J Cell Biol Vol. 215 ( 3 ) page： 357 2016

The microtubule catastrophe promoter Sentin delays stable kinetochore-microtubule attachment in oocytes. Reviewed Open Access

Głuszek AA, Cullen CF, Li W, Battaglia RA, Radford SJ, Costa MF, McKim KS, Goshima G, Ohkura H.

J Cell Biol Vol. 211 page： 1113-20 2015.12

Microcephaly protein Asp focuses the minus ends of spindle microtubules at the pole and within the spindle. Reviewed Open Access

Ito A, Goshima G.

J. Cell Biol. Vol. 211 page： 999-1009 2015.12

Clustering of a kinesin-14 motor enables processive retrograde microtubule-based transport in plants Open Access

Jonsson E, Yamada M, Vale RD, Goshima G.

Nature Plants Vol. 1 page： 15087 2015.6

RNAi screening identifies the armadillo repeat-containing kinesins responsible for microtubule-dependent nuclear positioning in Physcomitrella patens.

Miki T, Nishina M, Goshima G.

Plant Cell Physiol. Vol. 56 ( 4 ) page： 737-749 2015.1

Cytoplasmic nucleation and atypical branching nucleation generate endoplasmic microtubules in Physcomitrella patens Reviewed

Nakaoka Y, Kimura A, Tani T, Goshima G.

Plant Cell Vol. 27 ( 1 ) page： 228-242 2015.1

NACK kinesin is required for metaphase chromosome alignment and cytokinesis in the moss Physcomitrella patens.

Naito H, Goshima G.

Cell Structure and Function. Vol. 40 ( 1 ) page： 31-41 2015

Gohta Goshima: questing for answers on the mitotic spindle Open Access

Goshima G, Sedwick C.

J Cell Biol 2014.7

Identification of the augmin complex in the filamentous fungus Aspergillus nidulans. Open Access

Edzuka T, Yamada L, Kanamaru K, Sawada H, Goshima G.

PLoS One 2014.7

Endogenous localizome identifies 43 mitotic kinesins in a plant cell Open Access

Miki T, Naito H, Nishina M, Goshima G.

Proc Natl Acad Sci U S A 2014.5

Friction on MAP determines its traveling direction on microtubules. Open Access

2014.4

Genes involved in centrosome-independent mitotic spindle assembly in Drosophila S2 cells Open Access

Proc Natl Acad Sci U S A Vol. 110 ( 49 ) page： 19808-13 2013.12

Loss of a Rho-Regulated Actin Nucleator, mDia2, Impairs cytokinesis during mouse fetal erythropoiesis.

Watanabe S, De Zan T, Ishizaki T, Yasuda S, Kamijo H, Yamada D, Aoki T, Kiyonari H, Kaneko H, Shimizu R, Yamamoto M, Goshima G, Narumiya S.

Vol. 5 ( 4 ) page： 926-32 2013.11

MICROTUBULE-ASSOCIATED PROTEIN65 is essential for maintenance of phragmoplast bipolarity and formation of the cell plate in Physcomitrella patens. Open Access

Kosetsu K, de Keijzer J, Janson ME, Goshima G.

Plant Cell 2013.11

Aurora B and Kif2A control microtubule length for assembly of a functional central spindle during anaphase. Reviewed Open Access

Uehara R, Tsukada Y, Kamasaki T, Poser I, Yoda K, Gerlich DW, Goshima G.

Vol. 202 ( 4 ) page： 623-36 2013

Augmin-dependent microtubule nucleation at microtubule walls in the spindle. Reviewed Open Access

Kamasaki T, O'Toole E, Kita S, Osumi M, Usukura J, McIntosh JR, Goshima G.

J. Cell Biol. Vol. 202 ( 1 ) page： 25-33 2013

An inducible RNA interference system in Physcomitrella patens reveals a dominant role of augmin in phragmoplast microtubule generation Reviewed

Nakaoka Y, Miki T, Fujioka R, Uehara R, Tomioka A, Obuse C, Kubo M, Hiwatashi Y, Goshima G.

Plant Cell Vol. 24 ( 4 ) page： 1478-93 2012.4

Reconstitution of dynamic microtubules with Drosophila XMAP215, EB1, and Sentin Reviewed

Li W, Moriwaki T, Tani T, Watanabe T, Kaibuchi K, Goshima G.

J. Cell Biol. Vol. 199 ( 5 ) page： 849-62 2012

Identification of a TPX2-like microtubule-associated protein in Drosophila. Reviewed

Goshima G.

PLoS One Vol. 6 page： e28120 2011.11

EB1 promotes microtubule dynamics by recruiting Sentin in Drosophila cells. Reviewed Open Access

Li W, Miki T, Watanabe T, Kakeno M, Sugiyama I, Kaibuchi K, Goshima G.

J Cell Biol. Vol. 193 ( 6 ) page： 973-983 2011.6

Control of mitotic spindle length. Invited Reviewed

Goshima G, Scholey JM.

Annu Rev Cell Dev Biol Vol. 26 page： 21-57 2010.11

Functional central spindle assembly requires de novo microtubule generation in the interchromosomal region during anaphase. Reviewed Open Access

Uehara R, Goshima G.

Journal of Cell Biology Vol. 191 page： 259-267 2010.10

Determinants of myosin II cortical localization during cytokinesis. Reviewed

Uehara R, Goshima G, Mabuchi I, Vale RD, Spudich JA, Griffis ER.

Current Biology Vol. 22 page： 1080-1085 2010.6

*New look inside the spindle: microtubule-dependent microtuble generation within the spindle. Invited Reviewed

Goshima G, Kimura A

Current Opinion in Cell Biology Vol. Epub 2010.2

*The augmin complex plays a critical role in spindle microtubule generation for mitotic progression and cytokinesis in human cells. Reviewed Open Access

Uehara R, Nozawa RS, Tomioka A, Petry S, Vale RD, Obuse C, Goshima G.

Proc Natl Acad Sci U S A. Vol. 106 page： 6998-7003 2009.4

*Augmin: a protein complex required for centrosome-independent microtubule generation within the spindle Reviewed

Gohta Goshima, Mirjam Mayer, Nan Zhang, Nico Stuurman, Ronald D. Vale

Journal of Cell Biology Vol. 181 page： 421-429 2008.5

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

Since the discovery of gamma-tubulin, attention has focused on its involvement as a microtubule nucleator at the centrosome. However, mislocalization of gamma-tubulin away from the centrosome does not inhibit mitotic spindle formation in Drosophila melanogaster, suggesting that a critical function for gamma-tubulin might reside elsewhere. A previous RNA interference (RNAi) screen identified five genes (Dgt2-6) required for localizing gamma-tubulin to spindle microtubules. We show that the Dgt proteins interact, forming a stable complex. We find that spindle microtubule generation is substantially reduced after knockdown of each Dgt protein by RNAi. Thus, the Dgt complex that we name "augmin" functions to increase microtubule number. Reduced spindle microtubule generation after augmin RNAi, particularly in the absence of functional centrosomes, has dramatic consequences on mitotic spindle formation and function, leading to reduced kinetochore fiber formation, chromosome misalignment, and spindle bipolarity defects. We also identify a functional human homologue of Dgt6. Our results suggest that an important mitotic function for gamma-tubulin may lie within the spindle, where augmin and gamma-tubulin function cooperatively to amplify the number of microtubules.

DOI： 10.1016/j.yexcr.2008.12.024

Functional genomic screen reveals genes involved in lipid-droplet formation and utilization. Reviewed Open Access

Guo Y, Walther TC, Rao M, Stuurman N, Goshima G, Terayama K, Wong JS, Vale RD, Walter P, Farese RV.

Nature Vol. 453 ( 7195 ) page： 657-661 2008.5

Language：English Publishing type：Research paper (scientific journal)

Eukaryotic cells store neutral lipids in cytoplasmic lipid droplets enclosed in a monolayer of phospholipids and associated proteins. These dynamic organelles serve as the principal reservoirs for storing cellular energy and for the building blocks for membrane lipids. Excessive lipid accumulation in cells is a central feature of obesity, diabetes and atherosclerosis, yet remarkably little is known about lipid-droplet cell biology. Here we show, by means of a genome-wide RNA interference (RNAi) screen in Drosophila S2 cells that about 1.5% of all genes function in lipid-droplet formation and regulation. The phenotypes of the gene knockdowns sorted into five distinct phenotypic classes. Genes encoding enzymes of phospholipid biosynthesis proved to be determinants of lipid-droplet size and number, suggesting that the phospholipid composition of the monolayer profoundly affects droplet morphology and lipid utilization. A subset of the Arf1-COPI vesicular transport proteins also regulated droplet morphology and lipid utilization, thereby identifying a previously unrecognized function for this machinery. These phenotypes are conserved in mammalian cells, suggesting that insights from these studies are likely to be central to our understanding of human diseases involving excessive lipid storage.

DOI： 10.1038/nature06928

*Genes required for mitotic spindle assembly in Drosophila S2 cells Reviewed Open Access

Gohta Goshima, Roy Wollman, Sarah S. Goodwin, Nan Zhang, Jonathan M. Scholey, Ronald D. Vale and Nico Stuurman.

Science Vol. 316 ( 5823 ) page： 417-421 2007.7

Authorship：Lead author Language：English Publishing type：Research paper (scientific journal)

The formation of a metaphase spindle, a bipolar microtubule array with centrally aligned chromosomes, is a prerequisite for the faithful segregation of a cell's genetic material. Using a full-genome RNA interference screen of Drosophila S2 cells, we identified about 200 genes that contribute to spindle assembly, more than half of which were unexpected. The screen, in combination with a variety of secondary assays, led to new insights into how spindle microtubules are generated; how centrosomes are positioned; and how centrioles, centrosomes, and kinetochores are assembled.

DOI： 10.1126/science.1141314

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Papers - GOSHIMA, Gohta