Updated on 2021/06/29

写真a

 
SATO, Yoshiaki
 
Organization
Nagoya University Hospital Maternity and Perinatal Care Center Lecturer
Graduate School
Graduate School of Medicine
Title
Lecturer

Degree 1

  1. Ph.D. ( 2008.1   Nagoya University ) 

Research Interests 1

  1. Perinatal brain injury, cell therapy, neuroprotection

Research Areas 2

  1. Others / Others  / Embryonic/Neonatal Medicine

  2. Life Science / Embryonic medicine and pediatrics

Research History 12

  1. Associate Professor, Division of Neonatology, Center for Maternal-Neonatal Care, Nagoya University Hospital

    2013.7

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    Country:Japan

  2. Assistant Professor, Maternity & Perinatal Care Center, Nagoya University Hospital, Nagoya, Japan

    2010.10

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    Country:Japan

  3. Assistant Professor, Maternity & Perinatal Care Center, Nagoya University Hospital, Nagoya, Japan

    2009.4 - 2010.9

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    Country:Japan

  4. (Postdoctoral) Research fellow

    2007.4 - 2009.3

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    Country:Japan

  5. 愛知県コロニー心身障害者コロニー中央病院 新生児科・非常勤医

    2006.1 - 2007.3

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    Country:Japan

  6. 愛知県厚生連安城更生病院 小児科・医員

    2002.10 - 2004.3

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    Country:Japan

  7. Fellowship in Pediatrics, Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan

    2002.4 - 2002.9

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    Country:Japan

  8. Fellowship in Pediatrics, Department of Pediatrics, Nagoya University Graduate School of Medicine, Nagoya, Japan

    2002.1 - 2002.3

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    Country:Japan

  9. Fellowship in Neonatology, Facility of Perinatal Medicine, Department of Neonatology, Kanagawa Children's Medical Center, Yokohama, Japan

    2000.4 - 2001.12

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    Country:Japan

  10. Fellowship in Pediatrics, Anjo Kosei Hospital, Anjo, Aichi, Japan

    1999.5 - 2000.3

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    Country:Japan

  11. Residency in Pediatrics, Anjo Kosei Hospital, Anjo, Aichi, Japan

    1998.4 - 1999.4

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    Country:Japan

  12. Residency in Internal Medicine, Surgery, Obstetrics and Gynecology, Anesthesiology, Primary Care, ER, Anjo Kosei Hospital, Anjo, Japan

    1997.5 - 1998.3

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    Country:Japan

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Education 2

  1. Nagoya University   Graduate School, Division of Medical Sciences   Pediatrics

    2003.4 - 2007.3

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    Country: Japan

  2. Nagoya University   Faculty of Medicine

    1991.4 - 1997.3

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    Country: Japan

Professional Memberships 11

  1. Japan Society of Perinatal and Neonatal of Medicine

    2010.4

  2. Japan Neuroscience Society

  3. Japan Society for Premature and Newborn Medicine

    2014.1

  4. 日本小児科学会

  5. The Japanese Society for Regenerative Medicine

  6. International Society for Stem Cell Research

  7. JAPAN PEDIATRIC SOCIETY

  8. JAPAN SOCIETY FOR NEONATAL HEALTH AND DEVELOPMENT

  9. THE JAPAN NEUROSCIENCE SOCIETY

  10. JAPAN SOCIETY OF PERINATAL AND NEONATAL MEDICINE

  11. THE JAPANESE SOCIETY FOR REGENERATIVE MEDICINE

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Awards 1

  1. 日本小児科学会学術研究賞

    2016.5   日本小児科学会  

    佐藤義朗

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    Award type:Award from Japanese society, conference, symposium, etc.  Country:Japan

 

Papers 72

  1. Impact of maternal hypertensive disorders of pregnancy on brain volumes at term-equivalent age in preterm infants: A voxel-based morphometry study.

    Ushida T, Kidokoro H, Nakamura N, Katsuki S, Imai K, Nakano-Kobayashi T, Moriyama Y, Sato Y, Hayakawa M, Natsume J, Kajiyama H, Kotani T

    Pregnancy hypertension   Vol. 25   page: 143 - 149   2021.6

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    DOI: 10.1016/j.preghy.2021.06.003

    PubMed

  2. Diverse actions of cord blood cell therapy for hypoxic-ischemic encephalopathy.

    Sato Y, Tsuji M

    Pediatrics international : official journal of the Japan Pediatric Society   Vol. 63 ( 5 ) page: 497 - 503   2021.5

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    DOI: 10.1111/ped.14604

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  3. Brain damage caused by neonatal hypoxia-ischemia and the effects of hypothermia in severe combined immunodeficient (SCID) mice.

    Ogawa Y, Tanaka E, Sato Y, Tsuji M

    Experimental neurology   Vol. 337   page: 113577   2021.3

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    DOI: 10.1016/j.expneurol.2020.113577

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  4. Intravenously delivered multilineage-differentiating stress enduring cells dampen excessive glutamate metabolism and microglial activation in experimental perinatal hypoxic ischemic encephalopathy. International journal

    Toshihiko Suzuki, Yoshiaki Sato, Yoshihiro Kushida, Masahiro Tsuji, Shohei Wakao, Kazuto Ueda, Kenji Imai, Yukako Iitani, Shinobu Shimizu, Hideki Hida, Takashi Temma, Shigeyoshi Saito, Hidehiro Iida, Masaaki Mizuno, Yoshiyuki Takahashi, Mari Dezawa, Cesar V Borlongan, Masahiro Hayakawa

    Journal of cerebral blood flow and metabolism : official journal of the International Society of Cerebral Blood Flow and Metabolism   Vol. 41 ( 7 ) page: 1707 - 1720   2021.7

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    Perinatal hypoxic ischemic encephalopathy (HIE) results in serious neurological dysfunction and mortality. Clinical trials of multilineage-differentiating stress enduring cells (Muse cells) have commenced in stroke using intravenous delivery of donor-derived Muse cells. Here, we investigated the therapeutic effects of human Muse cells in an HIE model. Seven-day-old rats underwent ligation of the left carotid artery then were exposed to 8% oxygen for 60 min, and 72 hours later intravenously transplanted with 1 × 104 of human-Muse and -non-Muse cells, collected from bone marrow-mesenchymal stem cells as stage-specific embryonic antigen-3 (SSEA-3)+ and -, respectively, or saline (vehicle) without immunosuppression. Human-specific probe revealed Muse cells distributed mainly to the injured brain at 2 and 4 weeks, and expressed neuronal and glial markers until 6 months. In contrast, non-Muse cells lodged in the lung at 2 weeks, but undetectable by 4 weeks. Magnetic resonance spectroscopy and positron emission tomography demonstrated that Muse cells dampened excitotoxic brain glutamatergic metabolites and suppressed microglial activation. Muse cell-treated group exhibited significant improvements in motor and cognitive functions at 4 weeks and 5 months. Intravenously transplanted Muse cells afforded functional benefits in experimental HIE possibly via regulation of glutamate metabolism and reduction of microglial activation.

    DOI: 10.1177/0271678X20972656

    PubMed

  5. ドップラーセンサを用いた新生児の心拍測定におけるテンプレートマッチング呼吸残差法の検討 Reviewed

    上林眞司、 森 智也、佐藤義朗

    電子情報通信学会論文誌 B   Vol. 104-B ( 3 ) page: 369 - 378   2021.3

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    Authorship:Last author   Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.14923/transcomj.2020JBP3039

  6. Non-invasive biosignals detection for continuous monitoring of a neonate using quartz crystal resonator Reviewed

    Shiro Watanabe, Yuichi Murozaki, Hirotaka Sugiura, Yoshiaki Sato, Kazuya Honbe, Fumihito Arai

    Sensors and Actuators A: Physical   Vol. 317   page: 112475 - 112475   2021.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier BV  

    DOI: 10.1016/j.sna.2020.112475

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  7. Metabolomic analysis and mass spectrometry imaging after neonatal stroke and cell therapies in mouse brains.

    Tanaka E, Ogawa Y, Fujii R, Shimonaka T, Sato Y, Hamazaki T, Nagamura-Inoue T, Shintaku H, Tsuji M

    Scientific reports   Vol. 10 ( 1 ) page: 21881   2020.12

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    DOI: 10.1038/s41598-020-78930-x

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  8. Mesenchymal stem/stromal cells stably transduced with an inhibitor of CC chemokine ligand 2 ameliorate bronchopulmonary dysplasia and pulmonary hypertension. Reviewed International journal

    Toshihiko Suzuki, Yoshiaki Sato, Hidenori Yamamoto, Taichi Kato, Yuma Kitase, Kazuto Ueda, Haruka Mimatsu, Yuichiro Sugiyama, Atsuto Onoda, Shigeki Saito, Yoshiyuki Takahashi, Takayuki Nakayama, Masahiro Hayakawa

    Cytotherapy   Vol. 22 ( 4 ) page: 180 - 192   2020.4

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    Perinatal bronchopulmonary dysplasia (BPD) is defined as lung injury in preterm infants caused by various factors, resulting in serious respiratory dysfunction and high mortality. The administration of mesenchymal stem/stromal cells (MSCs) to treat/prevent BPD has proven to have certain therapeutic effects. However, MSCs can only weakly regulate macrophage function, which is strongly involved in the development of BPD. 7ND-MSCs are MSCs transfected with 7ND, a truncated version of CC chemokine ligand 2 (CCL2) that promotes macrophage activation, using a lentiviral vector. In the present study, we show in a BPD rat model that 7ND-MSC administration, but not MSCs alone, ameliorated the impaired alveolarization evaluated by volume density and surface area in the lung tissue, as well as pulmonary artery remodeling and pulmonary hypertension induced by BPD. In addition, 7ND-MSCs, but not MSCs alone, reduced M1 macrophages and the messenger RNA expressions of interleukin-6 and CCL2 in the lung tissue. Thus, the present study showed the treatment effect of 7ND-MSCs in a BPD rat model, which was more effective than that of MSCs alone.

    DOI: 10.1016/j.jcyt.2020.01.009

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  9. Autologous cord blood cell therapy for neonatal hypoxic-ischaemic encephalopathy: a pilot study for feasibility and safety. Reviewed International journal

    Masahiro Tsuji, Mariko Sawada, Shinichi Watabe, Hiroyuki Sano, Masayo Kanai, Emi Tanaka, Satoshi Ohnishi, Yoshiaki Sato, Hisanori Sobajima, Takashi Hamazaki, Rintaro Mori, Akira Oka, Hiroyuki Ichiba, Masahiro Hayakawa, Satoshi Kusuda, Masanori Tamura, Makoto Nabetani, Haruo Shintaku

    Scientific reports   Vol. 10 ( 1 ) page: 4603 - 4603   2020.3

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    Neonatal hypoxic-ischaemic encephalopathy (HIE) is a serious condition; many survivors develop neurological impairments, including cerebral palsy and intellectual disability. Preclinical studies show that the systemic administration of umbilical cord blood cells (UCBCs) is beneficial for neonatal HIE. We conducted a single-arm clinical study to examine the feasibility and safety of intravenous infusion of autologous UCBCs for newborns with HIE. When a neonate was born with severe asphyxia, the UCB was collected, volume-reduced, and divided into three doses. The processed UCB was infused at 12-24, 36-48, and 60-72 hours after the birth. The designed enrolment was six newborns. All six newborns received UCBC therapy strictly adhering to the study protocol together with therapeutic hypothermia. The physiological parameters and peripheral blood parameters did not change much between pre- and postinfusion. There were no serious adverse events that might be related to cell therapy. At 30 days of age, the six infants survived without circulatory or respiratory support. At 18 months of age, neurofunctional development was normal without any impairment in four infants and delayed with cerebral palsy in two infants. This pilot study shows that autologous UCBC therapy is feasible and safe.

    DOI: 10.1038/s41598-020-61311-9

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  10. A Novel Treatment with Stem Cells from Human Exfoliated Deciduous Teeth for Hypoxic-Ischemic Encephalopathy in Neonatal Rats. Reviewed International journal

    Yuma Kitase, Yoshiaki Sato, Kazuto Ueda, Toshihiko Suzuki, Alkisti Mikrogeorgiou, Yuichiro Sugiyama, Kohki Matsubara, Yuka Tsukagoshi Okabe, Shinobu Shimizu, Hitoshi Hirata, Hiroshi Yukawa, Yoshinobu Baba, Masahiro Tsuji, Yoshiyuki Takahashi, Akihito Yamamoto, Masahiro Hayakawa

    Stem cells and development   Vol. 29 ( 2 ) page: 63 - 74   2020.1

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    Recently, cell therapy has been developed as a novel treatment for perinatal hypoxic-ischemic encephalopathy (HIE), which is an important cause of neurological disorder and death, and stem cells from human exfoliated deciduous teeth (SHED) express early markers for mesenchymal and neuroectodermal stem cells. We investigated the treatment effect of SHED for HIE in neonatal rats. Seven-day-old rats underwent ligation of the left carotid artery and were exposed to 8% hypoxic treatment. SHED (1 × 105 cells) were injected via the right external jugular vein 24 h after the insult. The effect of intravenous administration of SHED cells was evaluated neurologically and pathophysiologically. In the evaluation of engraftment using quantum dots 655, only a few SHED were detected in the injured cortex. In the immunohistological evaluation 24 h after injection, the numbers of positive cells of active caspase-3 and anti-4 hydroxynonenal antiserum were lower in the SHED group than in the vehicle group. The number of Iba-1+ cells in the cortex was higher in the SHED group. However, the proportion of M1 microglia (Iba-1+/ED-1+) was significantly decreased, whereas M2 microglia (Iba-1+/CD206+) tended to increase in the SHED group. In the behavioral tests performed 5 months after hypoxic treatment, compared to the vehicle group, the SHED group showed significant elongation of the endurance time in the rotarod treadmill test, significantly ameliorated proportion of using the impaired hand in the cylinder test, significantly lower ratio of right/left front paw area in gait analysis, and significantly higher avoidance rate in the active avoidance test. In the in vitro experiment with cultured neurons exposed to oxygen-glucose deprivation, we confirmed the neuroprotective effect of the condition medium of SHED. These results suggested that intravenous administration of SHED exerted a treatment effect both histologically and functionally, possibly via a paracrine effect.

    DOI: 10.1089/scd.2019.0221

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  11. Establishment of a Novel Fetal Growth Restriction Model and Development of a Stem-Cell Therapy Using Umbilical Cord-Derived Mesenchymal Stromal Cells. Reviewed International journal

    Yuma Kitase, Yoshiaki Sato, Sakiko Arai, Atsuto Onoda, Kazuto Ueda, Shoji Go, Haruka Mimatsu, Mahboba Jabary, Toshihiko Suzuki, Miharu Ito, Akiko Saito, Akihiro Hirakawa, Takeo Mukai, Tokiko Nagamura-Inoue, Yoshiyuki Takahashi, Masahiro Tsuji, Masahiro Hayakawa

    Frontiers in cellular neuroscience   Vol. 14   page: 212 - 212   2020

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Fetal growth restriction (FGR) is a major complication of prenatal ischemic/hypoxic exposure and affects 5%-10% of pregnancies. It causes various disorders, including neurodevelopmental disabilities due to chronic hypoxia, circulatory failure, and malnutrition via the placenta, and there is no established treatment. Therefore, the development of treatments is an urgent task. We aimed to develop a new FGR rat model with a gradual restrictive load of uterus/placental blood flow and to evaluate the treatment effect of the administration of umbilical cord-derived mesenchymal stromal cells (UC-MSCs). To create the FGR rat model, we used ameroid constrictors that had titanium on the outer wall and were composed of C-shaped casein with a notch and center hole inside that gradually narrowed upon absorbing water. The ameroid constrictors were attached to bilateral ovarian/uterine arteries on the 17th day of pregnancy to induce chronic mild ischemia, which led to FGR with over 20% bodyweight reduction. After the intravenous administration of 1 × 105 UC-MSCs, we confirmed a significant improvement in the UC-MSC group in a negative geotaxis test at 1 week after birth and a rotarod treadmill test at 5 months old. In the immunobiological evaluation, the total number of neurons counted via the stereological counting method was significantly higher in the UC-MSC group than in the vehicle-treated group. These results indicate that the UC-MSCs exerted a treatment effect for neurological impairment in the FGR rats.

    DOI: 10.3389/fncel.2020.00212

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  12. Risk factors for absence of catch-up growth in small for gestational age very low-birthweight infants. Reviewed International journal

    Sakiko Arai, Yoshiaki Sato, Hideki Muramatsu, Hidenori Yamamoto, Fumiko Aoki, Yu Okai, Shinsuke Kataoka, Yu Hanada, Motoharu Hamada, Yoshihito Morimoto, Seiji Kojima, Jun Natsume, Yoshiyuki Takahashi

    Pediatrics international : official journal of the Japan Pediatric Society   Vol. 61 ( 9 ) page: 889 - 894   2019.9

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    BACKGROUND: Many small for gestational age (SGA) infants have catch-up growth during the first 2 years of life, but approximately 10% have no catch-up growth, and short stature continues into adulthood. Identification of risk factors for absence of catch-up growth at an early age may be useful for earlier diagnosis and earlier treatment. METHODS: This was a retrospective multicenter study. The subjects were SGA infants with very low-birthweight (VLBW), who were followed up until the age of 3 years. The risk factors for absence of catch-up growth were identified on statistical analysis. RESULTS: Of the 217 SGA infants in this study, 181 were in the catch-up group and 36 were in the no catch-up group. The catch-up rate was 83%. On multivariate analysis adjusted for gestational age, birthweight, birth height, and birth head circumference, multipara, Z and ΔZ scores of length at 12 months of corrected age, and the Z score of height at 24 months of corrected age were risk factors for lack of catch-up at 3 years. CONCLUSIONS: The length Z and ΔZ scores at 12 months of corrected age may be useful for an earlier diagnosis and earlier initiation of growth hormone treatment in VLBW infants.

    DOI: 10.1111/ped.13939

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  13. Comparison of Clinical Characteristics of Human Metapneumovirus and Respiratory Syncytial Virus Infections in Hospitalized Young Children. Reviewed

    Akinobu Taniguchi, Jun-Ichi Kawada, Kiyotaka Go, Naozumi Fujishiro, Yosuke Hosokawa, Yuki Maki, Yuichiro Sugiyama, Michio Suzuki, Takeshi Tsuji, Shin Hoshino, Hideki Muramatsu, Hiroyuki Kidokoro, Fumie Kinoshita, Akihiro Hirakawa, Yoshiyuki Takahashi, Yoshiaki Sato, Jun Natsume

    Japanese journal of infectious diseases   Vol. 72 ( 4 ) page: 237 - 242   2019.7

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    Human metapneumovirus (HMPV) and respiratory syncytial virus (RSV) are the leading causes of acute respiratory tract infection in children, and clinical manifestations of these virus infections are considered similar. To investigate the differences in clinical characteristics between HMPV and RSV infections in young children, we prospectively enrolled children < 3 years old who required hospitalization with acute respiratory tract infection due to HMPV or RSV at 10 hospitals in Japan. We enrolled 48 children with HMPV infection and 141 with RSV infection. Patients with HMPV infection were older than those with RSV infection. High-grade fever was more frequently observed in patients with HMPV infection, whereas no significant differences in respiratory symptoms were apparent. Abnormal serum lactate dehydrogenase values and consolidation shadows on chest X-ray were more frequently observed in patients with HMPV infection. During hospitalization, nasal mucus suction was more frequently required in patients with RSV infection. On the other hand, β2-adrenergic agonists, corticosteroids, and leukotriene receptor antagonists were more frequently used in patients with HMPV infection. These findings suggest that HMPV and RSV infections show similar respiratory symptoms, but HMPV infection is more likely to lead to the development of pneumonia, at least among hospitalized young children.

    DOI: 10.7883/yoken.JJID.2018.480

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  14. Factors related to survival discharge in trisomy 18: A retrospective multicenter study. Reviewed International journal

    Eiko Kato, Yuma Kitase, Takashi Tachibana, Tetsuo Hattori, Akiko Saito, Yukako Muramatsu, Koji Takemoto, Hikaru Yamamoto, Seiji Hayashi, Ayako Yasuda, Yuichi Kato, Kuniko Ieda, Makoto Oshiro, Yoshiaki Sato, Masahiro Hayakawa

    American journal of medical genetics. Part A   Vol. 179 ( 7 ) page: 1253 - 1259   2019.7

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    Infants with trisomy 18 (T18) previously had a poor prognosis; however, the intensive care of these patients has markedly diversified the prognosis. We investigated the current situation of patients with T18, clarified factors for survival discharge, and surveyed actual home healthcare. A total of 117 patients with T18 admitted to nine institutions between 2000 and 2015 were retrospectively investigated. After excluding four patients whose outcomes were unclear, we divided 113 patients into two groups-the survival discharge group (n = 52) and the death discharge group (n = 61)-and compared maternal factors, perinatal factors, neonatal factors, and therapeutic factors between the groups. In addition, home healthcare, readmission, utilization of respite care and home nursing, and cause of death among the survival group were surveyed. Fifty-two (44%) patients with T18 survived at discharge and their 1-year survival rate was 29%. The survival group had a longer gestation period, larger physique, and longer survival time, compared to the death group. Independent factors associated with survival discharge were the absence of an extremely low birthweight infant (ELBWI), the absence of esophageal atresia and patent ductus arteriosus, and cardiovascular surgery. All surviving patients required some home healthcare. The most frequent cause of death was a respiratory disorder. We recommend discussing the treatment strategy with families in the presence of neonatologists or pediatric surgeons, who can explain differences in prognosis, based on the gestation period, birthweight, severity of cardiovascular disease, and cardiovascular surgery.

    DOI: 10.1002/ajmg.a.61146

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  15. Design of a prospective multicenter randomized controlled trial evaluating the effects of gastric lavage on coffee-ground emesis in neonates: study protocol. Reviewed

    Takashi Maeda, Yoshiaki Sato, Akihiro Hirakawa, Masahiro Nakatochi, Fumie Kinoshita, Takeshi Suzuki, Shintaro Ichimura, Ryoichi Ito, Ryuji Kudo, Michio Suzuki, Shin Hoshino, Yuichiro Sugiyama, Hideki Muramatsu, Hiroyuki Kidokoro, Jun-Ichi Kawada, Yoshiyuki Takahashi

    Nagoya journal of medical science   Vol. 81 ( 2 ) page: 227 - 232   2019.5

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    Neonates who swallow a considerable amount of maternal blood may exhibit vomiting and suckling disorder during the first few days of the postnatal period. Some clinicians treat these neonates with gastric lavage (GL) to prevent vomiting and the establishment of enteral feeding empirically, but there was no study assessing the effect of GL for neonates with coffee-ground emesis. We designed a multicenter randomized controlled trial to evaluate the efficacy and safety of GL in neonates with coffee-ground emesis. Vigorous neonates with birth weight ranging from 2500 g to 3999 g and gestational age between 37w0d and 41w6d who presented with coffee-ground emesis on more than twice and diagnosed as false melena, were divided into two groups using computerized randomization. We defined feeding intolerance (FI) as (1) ≥2 vomiting episodes in 4h or ≥3 episodes in 24h and/or (2) feeding failure on at least two occasions because of retching or poor sucking. Primary outcome is percentage of infants who present FI within 24 hours from admission. We also assessed the residual volumes, number of vomiting episodes, percentage of weight reduction at postnatal day 4, rates of body weight gain at 1 month of age, and peak serum total bilirubin value before discharge. To our knowledge, this is the first study to evaluate the safety and efficacy of GL for neonates with coffee-ground emesis. This trial is registered at UMIN Clinical Trials Registry as UMIN000026483.

    DOI: 10.18999/nagjms.81.2.227

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  16. Comparison of high-dose and low-dose corticosteroid therapy for refractory Mycoplasma pneumoniae pneumonia in children. Reviewed International journal

    Toshihiko Okumura, Jun-Ichi Kawada, Masaharu Tanaka, Kotaro Narita, Tomonori Ishiguro, Yuji Hirayama, Sho Narahara, Genki Tsuji, Yuichiro Sugiyama, Michio Suzuki, Takeshi Tsuji, Shin Hoshino, Masahiro Nakatochi, Hideki Muramatsu, Hiroyuki Kidokoro, Yoshiyuki Takahashi, Yoshiaki Sato

    Journal of infection and chemotherapy : official journal of the Japan Society of Chemotherapy   Vol. 25 ( 5 ) page: 346 - 350   2019.5

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    BACKGROUND: Mycoplasma pneumoniae pneumonia (MPP) is generally a self-limiting disease, but it may become refractory. It is thought that refractory MPP is linked to the excessive immunologic responses of the host. Consequently, the use of adjunctive systemic corticosteroids may have beneficial effects. In this study, we compared the effects of high- and low-dose corticosteroid therapy in a pediatric population with refractory MPP. METHODS: We retrospectively collected data from 91 pediatric MPP patients treated with adjunctive systemic corticosteroids between April 2014 and October 2016. The patients were divided into the following two groups: high-dose corticosteroid group (2 mg/kg/day or more of prednisolone equivalents; n = 38) and low-dose corticosteroid group (<2 mg/kg/day; n = 53). Additionally, we compared the number of febrile days post-corticosteroid administration. We used 25 paired patients in a propensity score matching analysis to correct for confounding factors both by age and by days (from onset till corticosteroid therapy initiation). RESULTS: We observed that in the high-dose corticosteroid group defervescence following corticosteroid therapy initiation was achieved significantly earlier and length of hospitalization was significantly shorter (0.8 ± 1.0 vs. 1.5 ± 1.4 days and 8.2 ± 2.4 vs. 10.7 ± 2.7 days, respectively). In the propensity score matching, we observed that significant differences in the length of fever following corticosteroid therapy initiation and hospitalization were still present. Further, neither of the groups developed corticosteroid-related adverse events. CONCLUSION: Our results suggest that patients with refractory MPP treated with high-dose corticosteroid could achieve defervescence earlier and have a shorter hospitalization.

    DOI: 10.1016/j.jiac.2019.01.003

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  17. Correction: Treatment with silver nitrate versus topical steroid treatment for umbilical granuloma: A non-inferiority randomized control trial. Reviewed International journal

    Chikako Ogawa, Yoshiaki Sato, Chiyo Suzuki, Azusa Mano, Atsushi Tashiro, Takafumi Niwa, Sayako Hamazaki, Yoshihiro Tanahashi, Midori Suzumura, Satoshi Hayano, Masahiro Hayakawa, Takeshi Tsuji, Shin Hoshino, Yuichiro Sugiyama, Hiroyuki Kidokoro, Jun-Ichi Kawada, Hideki Muramatsu, Akihiro Hirakawa, Masahiko Ando, Jun Natsume, Seiji Kojima

    PloS one   Vol. 14 ( 6 ) page: e0218205   2019

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    Authorship:Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    [This corrects the article DOI: 10.1371/journal.pone.0192688.].

    DOI: 10.1371/journal.pone.0218205

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  18. Magnetic resonance spectroscopy in preterm infants: association with neurodevelopmental outcomes. Reviewed International journal

    Reina Hyodo, Yoshiaki Sato, Miharu Ito, Yuichiro Sugiyama, Chikako Ogawa, Hisashi Kawai, Toshiki Nakane, Akiko Saito, Akihiro Hirakawa, Hiroyuki Kidokoro, Jun Natsume, Masahiro Hayakawa

    Archives of disease in childhood. Fetal and neonatal edition   Vol. 103 ( 3 ) page: F238 - F244   2018.5

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    OBJECTIVE: To compare magnetic resonance spectroscopy (MRS) metabolite ratios in preterm infants at term-equivalent age with those in term infants and to evaluate the association between MRS metabolites and neurodevelopmental outcomes at 18 months corrected age in preterm infants. DESIGN: We studied infants born at a gestational age <37 weeks and weighing <1500 g during 2009-2013 using MRS at term-equivalent age. Infants with major brain abnormalities were excluded. The ratios of N-acetylaspartate (NAA) to creatine (Cre), NAA to choline-containing compounds (Cho) and Cho to Cre in the frontal white matter and thalamus were measured using multivoxel point-resolved proton spectroscopy sequence. Neurodevelopmental outcomes were assessed at 18 months corrected age. RESULTS: Thirty-three preterm infants and 16 term infants were enrolled in this study. Preterm infants with normal development at 18 months showed significantly lower NAA/Cho ratios in the frontal white matter than term infants. There were no differences in the Cre/Cho ratios between preterm and term infants. At 18 months corrected age, 9 preterm infants with a mild developmental delay showed significantly lower NAA/Cho ratios in the thalamus than 24 preterm infants with normal development. CONCLUSIONS: Preterm infants at term-equivalent age showed reduced MRS metabolites (NAA/Cho) compared with term infants. Decreased NAA/Cho ratios in the thalamus were associated with neurodevelopmental delay at 18 months corrected age in preterm infants.

    DOI: 10.1136/archdischild-2016-311403

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  19. Successful Infant Pneumonectomy with Unilateral Pulmonary Artery Occlusion Test. Reviewed

    Koji Kato, Taichi Kato, Satoshi Hayano, Yoshie Fukasawa, Atsushi Numaguchi, Tetsuo Hattori, Akiko Saito, Yoshiaki Sato, Masahiro Hayakawa

    International heart journal   Vol. 59 ( 1 ) page: 237 - 239   2018.1

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    The use of unilateral pulmonary artery occlusion (UPAO) test for the preoperative evaluation of pneumonectomy was reported in adult patients. On the contrary, in infants, no strategies have yet been recommended to predict hemodynamics after pneumonectomy, nor has use of the UPAO test been reported. We describe the first case of infant with abnormal pulmonary circulation in whom successful pneumonectomy was performed after preoperative evaluation using UPAO test. Right pneumonectomy was planned for an 8-month-old girl, because of decreased right pulmonary function, high risk of pneumothorax, and impaired left lung expansion due to overexpansion caused by severe left bronchial stenosis and bronchomalacia. However, she had also prolonged pulmonary hypertension and there was difficulty in accurate echocardiographic evaluation of its severity due to concomitant left pulmonary artery stenosis. Furthermore, contrast-enhanced computer tomography suggested a certain degree of right pulmonary venous flow, discordant with the result showing scarce right pulmonary flow in perfusion scintigraphy. Predicting postoperative hemodynamic changes was therefore considered difficult. To evaluate these concerns, we performed cardiac catheterization and UPAO test to simulate postoperative hemodynamics. Pulmonary arteriography showed decreased but significant right pulmonary arterial and venous flows. Measurements including pulmonary artery pressure and cardiac index showed no marked changes after occlusion. Based on UPAO test results, the operation was successfully performed and hemodynamics remained stable postoperatively. The UPAO test may be useful for infants with cardiopulmonary impairment to evaluate the tolerability of pneumonectomy.

    DOI: 10.1536/ihj.16-606

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    Other Link: http://orcid.org/0000-0001-6320-9176

  20. Neural stem/progenitor cells for perinatal brain injury Reviewed

    Yoshiaki Sato

    Cell Therapy for Perinatal Brain Injury     page: 37 - 43   2018.1

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    Cell transplantation therapy is a potential treatment for neonatal brain injury. Neural stem/progenitor cells (NSPCs) are defined as cells that have abilities for self-renewal and differentiation into neurons, astrocytes, and oligodendrocytes. There are several origins of NSPCs, namely, ES cell-derived NSPCs, iPS-derived NSPCs, fetal NSPCs, and adult NSPCs. NSPCs were formerly the cell type most commonly studied in the early period in the stem cell research to develop novel therapies for neuronal diseases. There are several experimental studies using NSPCs for the treatment of perinatal brain injuries. However, there has been only one clinical trial using NSPCs for neonatal brain injury to date. In the clinical application of NSPC treatment for neonatal brain injury, we would encounter greater difficulty due to ethical considerations when preparing the cells. In addition, we must consider the invasive approach for injection of cells. In this chapter, I describe the various origins of NSPCs as well as preclinical and clinical studies involving NSPCs.

    DOI: 10.1007/978-981-10-1412-3_4

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  21. Other tissues-derived mesenchymal stem cells for perinatal brain injury Reviewed

    Yoshiaki Sato

    Cell Therapy for Perinatal Brain Injury     page: 69 - 76   2018.1

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    Cell-based treatments are emerging as a potential therapy for perinatal brain injury. Neural stem/progenitor cells (NSPCs) have been studied primarily in preclinical models of HIE. However, despite their neural commitment, ethical concerns hinder the use of postmortem human brains as a source of NSPCs in future clinical applications. Furthermore, intracerebral administration is an invasive procedure. Stem cells originating from nonneural tissues can provide an approach that avoids these limitations. The stem cells from nonneural tissues, such as umbilical cord blood cells (UCBCs) and mesenchymal stem cells (MSCs), have a broad potential for differentiation, into mesodermal, endodermal, and ectodermal lineages. UCBCs have an advantage in that we can perform autologous transplantation if we get them at birth
    however, it is conceivable that we might often encounter difficulties collecting UCBCs in emergency situations. MSCs can be used as alternative cells for the treatment of perinatal HIE because we can use allogeneic MSCs. In this chapter, I describe preclinical studies with various origins of MSCs as well as clinical studies.

    DOI: 10.1007/978-981-10-1412-3_7

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  22. Dose-Dependent Effect of Intravenous Administration of Human Umbilical Cord-Derived Mesenchymal Stem Cells in Neonatal Stroke Mice. Reviewed International journal

    Emi Tanaka, Yuko Ogawa, Takeo Mukai, Yoshiaki Sato, Takashi Hamazaki, Tokiko Nagamura-Inoue, Mariko Harada-Shiba, Haruo Shintaku, Masahiro Tsuji

    Frontiers in neurology   Vol. 9   page: 133 - 133   2018

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    Neonatal brain injury induced by stroke causes significant disability, including cerebral palsy, and there is no effective therapy for stroke. Recently, mesenchymal stem cells (MSCs) have emerged as a promising tool for stem cell-based therapies. In this study, we examined the safety and efficacy of intravenously administered human umbilical cord-derived MSCs (UC-MSCs) in neonatal stroke mice. Pups underwent permanent middle cerebral artery occlusion at postnatal day 12 (P12), and low-dose (1 × 104) or high-dose (1 × 105) UC-MSCs were administered intravenously 48 h after the insult (P14). To evaluate the effect of the UC-MSC treatment, neurological behavior and cerebral blood flow were measured, and neuroanatomical analysis was performed at P28. To investigate the mechanisms of intravenously injected UC-MSCs, systemic blood flowmetry, in vivo imaging and human brain-derived neurotrophic factor (BDNF) measurements were performed. Functional disability was significantly improved in the high-dose UC-MSC group when compared with the vehicle group, but cerebral blood flow and cerebral hemispheric volume were not restored by UC-MSC therapy. The level of exogenous human BDNF was elevated only in the cerebrospinal fluid of one pup 24 h after UC-MSC injection, and in vivo imaging revealed that most UC-MSCs were trapped in the lungs and disappeared in a week without migration toward the brain or other organs. We found that systemic blood flow was stable over the 10 min after cell administration and that there were no differences in mortality among the groups. Immunohistopathological assessment showed that the percent area of Iba1-positive staining in the peri-infarct cortex was significantly reduced with the high-dose UC-MSC treatment compared with the vehicle treatment. These results suggest that intravenous administration of UC-MSCs is safe for a mouse model of neonatal stroke and improves dysfunction after middle cerebral artery occlusion by modulating the microglial reaction in the peri-infarct cortex.

    DOI: 10.3389/fneur.2018.00133

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  23. Treatment with silver nitrate versus topical steroid treatment for umbilical granuloma: A non-inferiority randomized control trial. Reviewed International journal

    Chikako Ogawa, Yoshiaki Sato, Chiyo Suzuki, Azusa Mano, Atsushi Tashiro, Takafumi Niwa, Sayako Hamazaki, Yoshihiro Tanahashi, Midori Suzumura, Satoshi Hayano, Masahiro Hayakawa, Takeshi Tsuji, Shin Hoshino, Yuichiro Sugiyama, Hiroyuki Kidokoro, Jun-Ichi Kawada, Hideki Muramatsu, Akihiro Hirakawa, Masahiko Ando, Jun Natsume, Seiji Kojima

    PloS one   Vol. 13 ( 2 ) page: e0192688   2018

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    OBJECTIVE: The aim of this prospective multicenter randomized controlled trial was to compare the efficacy of silver nitrate cauterization against that of topical steroid ointment in the treatment of neonatal umbilical granuloma. METHODS: An open-label, non-inferiority randomized controlled trial was conducted from January 2013 to January 2016. The primary endpoint for the silver nitrate cauterization and topical steroid ointment groups was the healing rate after 2 weeks of treatment, applying a non-inferiority margin of 10%. The healing rate was evaluated until completion of 3 weeks of treatment. RESULTS: Participants comprised 207 neonates with newly diagnosed umbilical granuloma, randomized to receive silver nitrate cauterization (n = 104) or topical steroid ointment (n = 103). Healing rates after 2 weeks of treatment were 87.5% (91/104) in the silver nitrate cauterization and 82% (82/100) in the topical steroid ointment group group. The difference between groups was -5.5% (95% confidence interval, -19.1%, 8.4%), indicating that the non-inferiority criterion was not met. After 3 weeks of treatment, the healing rate with topical steroid ointment treatment was almost identical to that of silver nitrate cauterization (94/104 [90.4%] vs. 91/100 [91.0%]; 0.6% [-13.2 to 14.3]). No major complications occurred in either group. CONCLUSIONS: This study did not establish non-inferiority of topical steroid ointment treatment relative to silver nitrate cauterization, presumably due to lower healing rates than expected leading to an underpowered trial. However, considering that silver nitrate cauterization carries a distinct risk of chemical burns and that the overall efficacy of topical steroid ointment treatment is similar to that of silver nitrate cauterization, topical steroid ointment might be considered as a good alternative in the treatment of neonatal umbilical granuloma due to its safety and simplicity. To clarify non-inferiority, a larger study is needed.

    DOI: 10.1371/journal.pone.0192688

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    Other Link: http://orcid.org/0000-0001-6320-9176

  24. Administration of Bone Marrow-Derived Mononuclear Cells Contributed to the Reduction of Hypoxic-Ischemic Brain Injury in Neonatal Rats. Reviewed International journal

    Yoshiaki Sato, Kazuto Ueda, Taiki Kondo, Tetsuo Hattori, Alkisti Mikrogeorgiou, Yuichiro Sugiyama, Toshihiko Suzuki, Michiro Yamamoto, Hitoshi Hirata, Akihiro Hirakawa, Keiko Nakanishi, Masahiro Tsuji, Masahiro Hayakawa

    Frontiers in neurology   Vol. 9   page: 987 - 987   2018

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    Background/Objective: Perinatal hypoxic-ischemia (HI) causes neonatal death and permanent neurological deficits. Cell therapy using various cell sources has been recently identified as a novel therapy for perinatal HI. Among the available types of cell sources, bone marrow-derived mononuclear cells (BMMNCs) have unique features for clinical application. For example, stem cells can be collected after admission, thus enabling us to perform autologous transplantation. This study aimed to investigate whether the administration of BMMNCs ameliorated HI brain injury in a neonatal rat model. Methods: Seven-day-old rats underwent left carotid artery ligation and were exposed to 8% oxygen for 60 min. BMMNCs were collected from the femurs and tibias of juvenile rats using the Ficoll-Hypaque technique and injected intravenously 24 h after the insult (1 × 105 cells). Active caspase-3, as an apoptosis marker, and ED1, as an activated microglia/macrophage marker, were evaluated immunohistochemically 48 h after the insult (vehicle, n = 9; BMMNC, n = 10). Behavioral assessments using the rotarod treadmill, gait analysis, and active avoidance tests were initiated 3 weeks after the insult (sham, n = 9, vehicle, n = 8; BMMNC, n = 8). After these behavioral tests (6 weeks after the insult), we evaluated the volumes of their hippocampi, cortices, thalami, striata, and globus pallidus. Results: The mean cell densities of the sum of four parts that were positive for active caspase-3 significantly decreased in the BMMNC group (p < 0.05), whereas in the hippocampi, cortices, thalami, and striata cell densities decreased by 42, 60, 56, and 47%, respectively, although statistical significance was not attained. The number of ED1 positive cells for the sum of the four parts also significantly decreased in the BMMNC group compared to the vehicle group (p < 0.05), whereas in each of the four parts the decrease was 35, 39, 47, and 36%, respectively, although statistical significance was not attained. In gait analysis, the BMMNC normalized the contact area of the affected hind paw widened by HI. The volumes of the affected striata and globus pallidus were significantly larger in the BMMNC group than in the control group. Conclusion: These results indicated that the injection of BMMNCs ameliorated HI brain injury in a neonatal rat model.

    DOI: 10.3389/fneur.2018.00987

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  25. Carbamylated Erythropoietin Decreased Proliferation and Neurogenesis in the Subventricular Zone, but Not the Dentate Gyrus, After Irradiation to the Developing Rat Brain. Reviewed International journal

    Kazuhiro Osato, Yoshiaki Sato, Akari Osato, Machiko Sato, Changlian Zhu, Marcel Leist, Hans G Kuhn, Klas Blomgren

    Frontiers in neurology   Vol. 9   page: 738 - 738   2018

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    Cranial radiotherapy for pediatric brain tumors causes progressive, debilitating late effects, including cognitive decline. Erythropoietin (EPO) has been shown to be neuroprotective and to promote neuroregeneration. Carbamylated erythropoietin (CEPO) retains the protective properties of EPO but is not erythrogenic. To study the effects of CEPO on the developing brain exposed to radiotherapy, a single irradiation (IR) dose of 6 Gy was administered to the brains of postnatal day 9 (P9) rats, and CEPO (40 μg/kg s.c.) was injected on P8, P9, P11, P13, and P15. To examine proliferation, 5-Bromo-2-deoxyuridine (BrdU) was injected on P15, P16, and P17. CEPO administration did not affect BrdU incorporation in the granule cell layer (GCL) of the hippocampus or in the subventricular zone (SVZ) as quantified 7 days after the last BrdU injection, whereas IR decreased BrdU incorporation in the GCL and SVZ by 63% and 18%, respectively. CEPO did not affect BrdU incorporation in the GCL of irradiated brains, although it was reduced even further (to 31%) in the SVZ. To evaluate the effect of CEPO on neurogenesis, BrdU/doublecortin double-positive cells were quantified. CEPO did not affect neurogenesis in non-irradiated brains, whereas IR decreased neurogenesis by 58% in the dentate gyrus (DG) but did not affect it in the SVZ. In the DG, CEPO did not affect the rate of neurogenesis following IR, whereas in the SVZ, the rate decreased by 30% following IR compared with the rate in vehicle-treated rats. Neither CEPO nor IR changed the number of microglia. In summary, CEPO did not promote neurogenesis in non-irradiated or irradiated rat brains and even aggravated the decreased neurogenesis in the SVZ. This raises concerns regarding the use of EPO-related compounds following radiotherapy.

    DOI: 10.3389/fneur.2018.00738

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  26. Ureteral dilatation detected in magnetic resonance imaging predicts vesicoureteral reflux in children with urinary tract infection. Reviewed International journal

    Norihiro Murakami, Jun-Ichi Kawada, Azumi Watanabe, Toshinao Arakawa, Takamasa Kano, Takako Suzuki, Ryo Tanaka, Daiei Kojima, Yoshihiko Kawano, Shin Hoshino, Hideki Muramatsu, Yoshiyuki Takahashi, Yoshiaki Sato, Masashi Koyama, Jun Natsume

    PloS one   Vol. 13 ( 12 ) page: e0209595   2018

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    OBJECTIVE: Urinary tract infection (UTI), one of the most common bacterial infections occurring during infancy and early childhood, is frequently associated with vesicoureteral reflux (VUR). Although several guidelines recommend performing ultrasonography as a screening test, its utility is not adequate and appropriate screening tests are strongly desirable. In this study, we evaluate the use of magnetic resonance imaging (MRI) as a screening test for VUR in children with UTI. METHODS: We prospectively studied 108 patients with suspected UTI between April 2014 and March 2016. UTI was diagnosed on the basis of diffusion-weighted MRI (DW-MRI) and urine culture findings. We measured ureteral dilatation using MRI in 96 patients with UTI and assessed the relationship between ureteral dilatation in MRI and VUR in 46 patients who underwent voiding cystourethrography (VCUG). RESULTS: Among 108 patients, 88 and 8 were diagnosed with upper and lower UTI, respectively. Among 46 patients who underwent VCUG, 23 had VUR (14 low grade and 9 high grade). Patients with ureteral dilatation detected on MRI had VUR more frequently than those without ureteral dilatation (any grades VUR, 71% vs. 32%; P = 0.02; high-grade VUR, 38% vs. 2%, P = 0.007). Overall, ureteral dilatation findings on MRI achieved sensitivity 65.2% and specificity 73.9% as a screening test for VUR. In addition, DW-MRI achieved sensitivity 100% and specificity 81.8% in the diagnosis of upper UTI. CONCLUSION: These findings suggested that MRI is a valuable tool for screening of VUR as well as diagnosis of upper UTI.

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  27. Intravenous Administration of Bone Marrow-Derived Mesenchymal Stem Cell, but not Adipose Tissue-Derived Stem Cell, Ameliorated the Neonatal Hypoxic-Ischemic Brain Injury by Changing Cerebral Inflammatory State in Rat. Reviewed International journal

    Yuichiro Sugiyama, Yoshiaki Sato, Yuma Kitase, Toshihiko Suzuki, Taiki Kondo, Alkisti Mikrogeorgiou, Asuka Horinouchi, Shoichi Maruyama, Yoshie Shimoyama, Masahiro Tsuji, Satoshi Suzuki, Tokunori Yamamoto, Masahiro Hayakawa

    Frontiers in neurology   Vol. 9   page: 757 - 757   2018

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    Perinatal hypoxic-ischemic (HI) brain injury occurs in 1 in 1,000 live births and remains the main cause of neurological disability and death in term infants. Cytotherapy has recently emerged as a novel treatment for tissue injury. In particular, mesenchymal stem cells (MSCs) are thought to have therapeutic potential, but little is known about the differences according to their origin. In the current study, we investigated the therapeutic effects and safety of intravenous injection of allogeneic bone marrow-derived MSCs (BM-MSCs) and adipose-derived stem cells (ADSCs) in a rat model of HI brain injury. HI models were generated by ligating the left carotid artery of postnatal day 7 Wistar/ST rats and exposing them to 8% hypoxia for 60 min. Bone marrow and adipose tissue were harvested from adult green fluorescent protein transgenic Wistar rats, and cells were isolated and cultured to develop BM-MSCs and ADSCs. At passaging stages 2-3, 1 × 105 cells were intravenously injected into the external right jugular vein of the HI rats at 4 or 24 h after hypoxia. Brain damage was evaluated by counting the number of cells positive for active caspase-3 in the entire dentate gyrus. Microglial isotypes and serum cytokines/chemokines were also evaluated. Distribution of each cell type after intravenous injection was investigated pathologically and bio-optically by ex vivo imaging (IVIS®) with a fluorescent lipophilic tracer DiR. The mortality rate was higher in the ADSC group compared to the BM-MSC group, in pups injected with cells 4 h after hypoxia. The number of active caspase-3-positive cells significantly decreased in the BM-MSC group, and the percentage of M1 microglia (a proinflammatory isotype) was also lower in the BM-MSC vs control group in the penumbra of the cortex. Moreover, BM-MSC administration increased anti-inflammatory cytokine and growth factor levels, while ADSCs did not. Each injected cell type was mainly distributed in the lungs and liver, but ADSCs remained in the lungs longer. Pathologically, pulmonary embolisms and diffuse alveolar hemorrhages were seen in the ADSC group. These results indicated that injection of allogeneic BM-MSCs ameliorated neonatal HI brain injury, whereas ADSCs induced severe lung hemorrhage and higher mortality.

    DOI: 10.3389/fneur.2018.00757

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  28. Grafting Neural Stem and Progenitor Cells Into the Hippocampus of Juvenile, Irradiated Mice Normalizes Behavior Deficits. Reviewed International journal

    Yoshiaki Sato, Noriko Shinjyo, Machiko Sato, Marie K L Nilsson, Kazuhiro Osato, Changlian Zhu, Marcela Pekna, Hans G Kuhn, Klas Blomgren

    Frontiers in neurology   Vol. 9   page: 715 - 715   2018

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    The pool of neural stem and progenitor cells (NSPCs) in the dentate gyrus of the hippocampus is reduced by ionizing radiation. This explains, at least partly, the learning deficits observed in patients after radiotherapy, particularly in pediatric cases. An 8 Gy single irradiation dose was delivered to the whole brains of postnatal day 9 (P9) C57BL/6 mice, and BrdU-labeled, syngeneic NSPCs (1.0 × 105 cells/injection) were grafted into each hippocampus on P21. Three months later, behavior tests were performed. Irradiation impaired novelty-induced exploration, place learning, reversal learning, and sugar preference, and it altered the movement pattern. Grafting of NSPCs ameliorated or even normalized the observed deficits. Less than 4% of grafted cells survived and were found in the dentate gyrus 5 months later. The irradiation-induced loss of endogenous, undifferentiated NSPCs in the dentate gyrus was completely restored by grafted NSPCs in the dorsal, but not the ventral, blade. The grafted NSPCs did not exert appreciable effects on the endogenous NSPCs; however, more than half of the grafted NSPCs differentiated. These results point to novel strategies aimed at ameliorating the debilitating late effects of cranial radiotherapy, particularly in children.

    DOI: 10.3389/fneur.2018.00715

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  29. Factors related to home health-care transition in trisomy 13. Reviewed International journal

    Yuma Kitase, Masahiro Hayakawa, Taiki Kondo, Akiko Saito, Takashi Tachibana, Makoto Oshiro, Kuniko Ieda, Eiko Kato, Yuichi Kato, Tetsuo Hattori, Seiji Hayashi, Masatoki Ito, Reina Hyodo, Yukako Muramatsu, Yoshiaki Sato

    American journal of medical genetics. Part A   Vol. 173 ( 10 ) page: 2635 - 2640   2017.10

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    Trisomy 13 (T13) is accompanied by severe complications, and it can be challenging to achieve long-term survival without aggressive treatment. However, recently, some patients with T13 have been receiving home care. We conducted this study to investigate factors related to home health-care transition for patients with T13.We studied 28 patients with T13 born between January 2000 and December 2014. We retrospectively compared nine home care transition patients (the home care group) and 19 patients that died during hospitalization (the discharge at death group). The median gestational age of the patients was 36.6 weeks, with a median birth weight of 2,047 g. Currently, three patients (11%) have survived, and 25 (89%) have died. The home care group exhibited a significantly longer gestational age (38.9 vs. 36.3 weeks, p = 0.039) and significantly larger occipitofrontal circumference Z score (-0.04 vs. -0.09, p = 0.019). Congenital heart defects (CHD) was more frequent in the discharge at death group, with six patients in the home care group and 18 patients in the discharge at death group (67% vs. 95%, p = 0.047), respectively. Survival time was significantly longer in the home care group than in the discharge at death group (171 vs. 19 days, p = 0.012). This study has shown that gestational age, occipitofrontal circumference Z score at birth, and the presence of CHD are helpful prognostic factors for determining treatment strategy in patients with T13.

    DOI: 10.1002/ajmg.a.38371

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  30. A new type of swaddling clothing improved development of preterm infants in neonatal intensive care units. Reviewed International journal

    Yuma Kitase, Yoshiaki Sato, Hirokazu Takahashi, Misaki Shimizu, Chie Ishikawa, Hikaru Yamamoto, Masahiro Hayakawa

    Early human development   Vol. 112   page: 25 - 28   2017.9

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    BACKGROUND: Preterm infants undergo stress owing to essential treatments and exposure to the extrauterine environment in neonatal intensive care units. AIMS: The aim of this study was to enable preterm infants to maintain adequate positioning with a newly developed swaddling clothing, in order to improve low muscle tone and sleep quality, and to confirm the safety of the clothing. STUDY DESIGN: This prospective clinical trial included an intervention group (preterm infants wearing bag-shaped clothing, allowing only exposure of the head, n=27), and a control group (preterm infants managed only with conventional swaddling, n=12). OUTCOME MEASURES: We used the Dubowitz method to analyze behavior, recorded the frequency of vomiting and apnea in both groups, and assessed the sleep state in the intervention group. RESULTS: Muscle tone and total score for the Dubowitz method significantly improved in the intervention group, compared with those in the control group. We evaluated the sleep state before and after the introduction of the device in the intervention group, and State 1 increased from 53.5% to 69.2% after introduction. No significant difference was seen in the frequency of vomiting and apnea between the groups. CONCLUSIONS: The new swaddling clothing with enhanced stretch capacity improved the muscle tone and increased sleep time by decreasing the state level of preterm infants. This is an effective tool to assist in infant development in neonatal intensive care units.

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  31. The relationship between three signs of fetal magnetic resonance imaging and severity of congenital diaphragmatic hernia Reviewed

    T. Hattori, M. Hayakawa, M. Ito, Y. Sato, K. Tamakoshi, Y. Kanamorit, H. Okuyama, N. Inannura, S. Takahashi, Y. Fujino, T. Taguchi, N. Usui

    JOURNAL OF PERINATOLOGY   Vol. 37 ( 3 ) page: 265 - 269   2017.3

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    OBJECTIVE: To seek a simple approach for prenatally classifying congenital diaphragmatic hernia (CDH) severity using fetal magnetic resonance imaging (MRI) markers.
    STUDY DESIGN: A retrospective, multicenter study using questionnaires to investigate fetal MRI findings. We included fetuses prenatally diagnosed with isolated left-sided CDH and delivered after 36 weeks of gestation. We focused on three fetal MRI morphological signs: incomplete pulmonary baseline (IPB), liver up (LU) and retrocardiac stomach (RCS). We also evaluated the fetal MRI score defined as the total number of positive signs; the primary outcome was survival at discharge.
    RESULTS: In 256 patients (from 56 institutions), IPB, LU and RCS findings correlated with lower survival: odds ratio (95% confidence interval), 0.16 (0.08 to 0.33); 0.24 (0.12 to 0.51); and 0.14 (0.07 to 0.28); respectively. Patients with higher fetal MRI scores had a higher mortality rate.
    CONCLUSION: IPB, LU and RCS on fetal MRI are related to CDH severity.

    DOI: 10.1038/jp.2016.208

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    Other Link: http://orcid.org/0000-0001-6320-9176

  32. Dedifferentiated Fat Cells as a Novel Source for Cell Therapy to Target Neonatal Hypoxic-Ischemic Encephalopathy

    Mikrogeorgiou, A., Sato, Y., Kondo, T., Hattori, T., Sugiyama, Y., Ito, M., Saito, A., Nakanishi, K., Tsuji, M., Kazama, T., Kano, K., Matsumoto, T., Hayakawa, M.

    Dev Neurosci     2017

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    DOI: 10.1159/000455836

  33. Rat umbilical cord blood cells attenuate hypoxic-ischemic brain injury in neonatal rats

    Nakanishi, K., Sato, Y., Mizutani, Y., Ito, M., Hirakawa, A., Higashi, Y.

    Sci Rep   Vol. 7   page: 44111   2017

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    DOI: 10.1038/srep44111

  34. Abnormal urinalysis on day 7 in patients with IgA vasculitis (Henoch-Schonlein purpura)

    Kawashima, N., Kawada, J. I., Nishikado, Y., Kitase, Y., Ito, S., Muramatsu, H., Sato, Y., Kato, T., Natsume, J., Kojima, S.

    Nagoya J Med Sci   Vol. 78 ( 4 ) page: 359-368   2016

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    DOI: 10.18999/nagjms.78.4.359

  35. Mild intrauterine hypoperfusion reproduces neurodevelopmental disorders observed in prematurity

    Ohshima, M., Coq, J. O., Otani, K., Hattori, Y., Ogawa, Y., Sato, Y., Harada-Shiba, M., Ihara, M., Tsuji, M.

    Sci Rep   Vol. 6   page: 39377   2016

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    DOI: 10.1038/srep39377

  36. Evaluations of Intravenous Administration of CD34+ Human Umbilical Cord Blood Cells in a Mouse Model of Neonatal Hypoxic-Ischemic Encephalopathy

    Ohshima, M., Taguchi, A., Sato, Y., Ogawa, Y., Saito, S., Yamahara, K., Ihara, M., Harada-Shiba, M., Ikeda, T., Matsuyama, T., Tsuji, M.

    Dev Neurosci   Vol. 38 ( 5 ) page: 331 - 341   2016

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    DOI: 10.1159/000454830

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  37. Buckling surgery and supplemental intravitreal bevacizumab or photocoagulation on stage 4 retinopathy of prematurity eyes

    Futamura, Y., Asami, T., Nonobe, N., Kachi, S., Ito, Y., Sato, Y., Hayakawa, M., Terasaki, H.

    Jpn J Ophthalmol   Vol. 59 ( 6 ) page: 378-88   2015

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    DOI: 10.1007/s10384-015-0401-5

  38. Administration of umbilical cord blood cells transiently decreased hypoxic-ischemic brain injury in neonatal rats

    Hattori, T., Sato, Y., Kondo, T., Ichinohashi, Y., Sugiyama, Y., Yamamoto, M., Kotani, T., Hirata, H., Hirakawa, A., Suzuki, S., Tsuji, M., Ikeda, T., Nakanishi, K., Kojima, S., Blomgren, K., Hayakawa, M.

    Dev Neurosci   Vol. 37 ( 2 ) page: 95-104   2015

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    DOI: 10.1159/000368396

  39. Maternal molecular hydrogen treatment attenuates lipopolysaccharide-induced rat fetal lung injury

    Hattori, Y., Kotani, T., Tsuda, H., Mano, Y., Tu, L., Li, H., Hirako, S., Ushida, T., Imai, K., Nakano, T., Sato, Y., Miki, R., Sumigama, S., Iwase, A., Toyokuni, S., Kikkawa, F.

    Free Radic Res   Vol. 49 ( 8 ) page: 1026-37   2015

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    DOI: 10.3109/10715762.2015.1038257

  40. Maternal molecular hydrogen administration on lipopolysaccharide-induced mouse fetal brain injury

    Nakano, T., Kotani, T., Mano, Y., Tsuda, H., Imai, K., Ushida, T., Li, H., Miki, R., Sumigama, S., Sato, Y., Iwase, A., Hirakawa, A., Asai, M., Toyokuni, S., Kikkawa, F.

    J Clin Biochem Nutr   Vol. 57 ( 3 ) page: 178-82   2015

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    DOI: 10.3164/jcbn.15-90

  41. Intraperitoneal and intravenous deliveries are not comparable in terms of drug efficacy and cell distribution in neonatal mice with hypoxia-ischemia

    Ohshima, M., Taguchi, A., Tsuda, H., Sato, Y., Yamahara, K., Harada-Shiba, M., Miyazato, M., Ikeda, T., Iida, H., Tsuji, M.

    Brain Dev   Vol. 37 ( 4 ) page: 376-86   2015

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.braindev.2014.06.010

  42. Multicenter observational study comparing sedation/analgesia protocols for laser photocoagulation treatment of retinopathy of prematurity

    Sato, Y., Oshiro, M., Takemoto, K., Hosono, H., Saito, A., Kondo, T., Aizu, K., Matsusawa, M., Futamura, Y., Asami, T., Terasaki, H., Hayakawa, M.

    J Perinatol   Vol. 35 ( 11 ) page: 965-9   2015

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/jp.2015.112

  43. Paradoxical downward seizure pattern on amplitude-integrated electroencephalogram Reviewed

    Ito, M. and Kidokoro, H. and Sugiyama, Y. and Sato, Y. and Natsume, J. and Watanabe, K. and Hayakawa, M.

    J Perinatol   Vol. 34 ( 8 ) page: 642-4   2014

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  44. Effects of intravenous administration of umbilical cord blood CD34 cells in a mouse model of neonatal stroke Reviewed

    Tsuji, M. and Taguchi, A. and Ohshima, M. and Kasahara, Y. and Sato, Y. and Tsuda, H. and Otani, K. and Yamahara, K. and Ihara, M. and Harada-Shiba, M. and Ikeda, T. and Matsuyama, T.

    Neuroscience   Vol. 263C   page: 148-158   2014

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    Language:English   Publishing type:Research paper (scientific journal)  

  45. Effect of placenta previa on neonatal respiratory disorders and amniotic lamellar body counts at 36-38weeks of gestation Reviewed

    Tsuda, H. and Kotani, T. and Sumigama, S. and Mano, Y. and Hua, L. and Hayakawa, H. and Hayakawa, M. and Sato, Y. and Kikkawa, F.

    Early Hum Dev   Vol. 90 ( 1 ) page: 51-4   2014

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    Language:English   Publishing type:Research paper (scientific journal)  

  46. Astroglial IFITM3 mediates neuronal impairments following neonatal immune challenge in mice. Reviewed

    Ibi D, Nagai T, Nakajima A, Mizoguchi H, Kawase T, Tsuboi D, Kano S, Sato Y, Hayakawa M, Lange UC, Adams DJ, Surani MA, Satoh T, Sawa A, Kaibuchi K, Nabeshima T, Yamada K.

    Glia   Vol. 61 ( 5 ) page: 679   2013

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1002/glia.22461

  47. Amniotic lamellar body count and congenital diaphragmatic hernia in humans and in a rat model Reviewed

    Watanabe, Y. and Tsuda, H. and Kotani, T. and Sumigama, S. and Mano, Y. and Hayakawa, M. and Sato, Y. and Kikkawa, F.

    Pediatr Res   Vol. 73 ( 3 ) page: 344-8   2013

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    Language:English   Publishing type:Research paper (scientific journal)  

  48. Grafting of neural stem and progenitor cells to the hippocampus of young, irradiated mice causes gliosis and disrupts the granule cell layer Reviewed

    Sato, Y. and Shinjyo, N. and Sato, M. and Osato, K. and Zhu, C. and Pekna, M. and Kuhn, H. G. and Blomgren, K.

    Cell Death Dis   Vol. 4   page: e591   2013

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  49. The diameter of the inferior vena cava provides a noninvasive way of calculating central venous pressure in neonates Reviewed

    Sato, Y. and Kawataki, M. and Hirakawa, A. and Toyoshima, K. and Kato, T. and Itani, Y. and Hayakawa, M.

    Acta Paediatr   Vol. 102 ( 6 ) page: e241-6   2013

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  50. Dexamethasone administration to the neonatal rat results in neurological dysfunction at the juvenile stage even at low doses Reviewed

    Ichinohashi, Y. and Sato, Y. and Saito, A. and Ito, M. and Watanabe, K. and Hayakawa, M. and Nakanishi, K. and Wakatsuki, A. and Oohira, A.

    Early Hum Dev   Vol. 89 ( 5 ) page: 283-8   2013

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  51. A highly-sulfated chondroitin sulfate, CS-E, adsorbs specifically to neurons with nuclear condensation. Reviewed

    Nakanishi K, Ito M, Sato Y, Oohira A.

    Neuroscience research     2012

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.neures.2012.08.009

  52. Congenital pancreatoblastoma associated with beta-catenin mutation Reviewed

    Ismael O, Shimada A, Hama A, Takahashi Y, Sato Y, Hayakawa M, Tsuchiya H, Tainaka T, Ono Y, Kaneko K, Ando H, Sato K, Kojima S.

    Pediatric blood & cancer.   Vol. 58 ( 5 ) page: 827   2012

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  53. Carbohydrate and energy metabolism in the brain of rats with thromboxane A2-induced fetal growth restriction. Reviewed

    Hayakawa M, Sato Y, Hattori T, Ichinohashi Y, Nakayama A, Yamamoto H, Hemmi H, Ito M, Ieda K, Kojima S.

    Pediatr Res.   Vol. 70 ( 1 ) page: 21-4   2011

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  54. Biological activities of highly sulfated chondroitin sulfate polysaccharides on neural cells. Reviewed

    Nakanishi, K.Sato, Y.Oohira, A.

    Research Advances in Neurochemistry, Global Research Network   Vol. 1   page: 1-10   2010

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  55. The diffusion weighted imaging for an early diagnosis of parasagittal injury Reviewed

    Tagaya, M.Sato, Y.Hayakawa, M.

    Pediatr Int.   Vol. 52 ( 2 ) page: 298-301   2010

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  56. Apoptosis-inducing factor deficiency decreases the proliferation rate and protects the subventricular zone against ionizing radiation Reviewed

    Osato, K.*Sato, Y.*(*equally contribution)Ochiishi,T.Osato, A.Zhu, C.Sato, M.Swanpalmer, J.Modjtahedi, N.Kroemer, G.Kuhn, H. G.Blomgren, K.

    Cell Death & Disease   Vol. 1   page: e84   2010

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  57. *Chondroitin Sulfate, a Major Niche Substance of Neural Stem Cells, and Cell Transplantation Therapy of Neurodegeneration Combined with Niche Modification. Invited Reviewed

    Sato Y, Oohira A.

    Curr Stem Cell Res Ther   Vol. 4 ( 3 ) page: 200-209   2009

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    Authorship:Lead author, Last author   Language:English  

  58. Male gender is related to the development of parenteral nutrition-associated cholestasis in neonates Reviewed

    Takemoto, K.Nakayama, A.Ito, M.Sato, Y.Saito, A.Tori, Y.Kaneko, K.Ando, H.Hayakawa, M.

    Journal of Neonatal-Perinatal Medicine   Vol. 2 ( 4 ) page: 247-251   2009

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  59. Behavioral abnormalities of fetal growth retardation model rats with reduced amounts of brain proteoglycans. Reviewed

    Saito A, Matsui F, Hayashi K, Watanabe K, Ichinohashi Y, Sato Y, Hayakawa M, Kojima S, Oohira A.

    Exp Neurol   Vol. 219 ( 1 ) page: 81-92   2009

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  60. 神経幹細胞を用いた新生児低酸素性虚血性脳症治療の可能性. Invited

    伊田みちる, 佐藤義朗, 大平敦彦

    日本周産期・新生児医学会雑誌   Vol. 44 ( 4 ) page: 872-876   2008

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  61. Delayed neurological signs following isolated parasagittal injury in asphyxia at term. Reviewed

    Sato Y, Hayakawa M, Iwata O, Okumura A, Kato T, Hayakawa F, Kubota T, Maruyama K, Hasegawa M, Sato M, Oshiro M, Kito O, Kojima S.

    Eur J Paediatr Neurol   Vol. 12 ( 5 ) page: 359-365   2008

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  62. *Reduction of brain injury in neonatal hypoxic-ischemic rats by intracerebroventricular injection of neural stem/progenitor cells together with chondroitinase ABC. Reviewed

    Sato Y, Nakanishi K, Hayakawa M, Kakizawa H, Saito A, Kuroda Y, Ida M, Tokita Y, Aono S, Matsui F, Kojima S, Oohira A.

    Reprod Sci   Vol. 15 ( 6 ) page: 613-620   2008

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  63. *A highly sulfated chondroitin sulfate preparation, CS-E, prevents excitatory amino acid-induced neuronal cell death. Reviewed

    Sato Y, Nakanishi K, Tokita Y, Kakizawa H, Ida M, Maeda H, Matsui F, Aono S, Saito A, Kuroda Y, Hayakawa M, Kojima S, Oohira A.

    J Neurochem   Vol. 104   page: 1565-1576   2008

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  64. A new method of blood sampling reduces pain for newborn infants: a prospective, randomized controlled clinical trial. Reviewed

    Sato Y, Fukasawa T, Hayakawa M, Yatsuya H, Hatakeyama M, Ogawa A, Kuno K.

    Early Hum Dev   Vol. 83   page: 389-394   2007

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

  65. Novel SLC12A1 (NKCC2) mutations in two families with Bartter syndrome type 1. Reviewed

    Adachi M, Asakura Y, Sato Y, Tajima T, Nakajima T, Yamamoto T, Fujieda K.

    Endocr J   Vol. 54   page: 1003-1007   2007

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  66. The MRI findings of the right-sided fetal lung can be used to predict postnatal mortality and the requirement for extracorporeal membrane oxygenation in isolated left-sided congenital diaphragmatic hernia. Reviewed

    Hayakawa M, Seo T, Itakua A, Hayashi S, Miyauchi M, Sato Y, Saito A, Nakayama A, Takemoto K, Hasegawa M, Kaneko K, Okada M, Hayakawa H, Sumigama S, Kikkawa F, Ando H, Kojima S.

    Pediatr Res   Vol. 62   page: 93-97   2007

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  67. Neuroprotective effect of nipradilol, an NO donor, on hypoxic-ischemic brain injury of neonatal rats. Reviewed

    Kakizawa H, Matsui F, Tokita Y, Hirano K, Ida M, Nakanishi K, Watanabe M, Sato Y, Okumura A, Kojima S, Oohira A.

    Early Hum Dev   Vol. 83   page: 535-540   2007

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    Language:English   Publishing type:Research paper (scientific journal)  

  68. Magnetic resonance imaging regional T1 abnormalities at term accurately predict motor outcome in preterm infants. Reviewed

    Nanba Y, Matsui K, Aida N, Sato Y, Toyoshima K, Kawataki M, Hoshino R, Ohyama M, Itani Y, Goto A, Oka A.

    Pediatrics   Vol. 120   page: e10-e19   2007

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  69. An animal model of intrauterine growth retardation induced by synthetic thromboxane a(2). Reviewed

    Hayakawa M, Takemoto K, Nakayama A, Saito A, Sato Y, Hasegawa M, Ieda K, Mimura S.

    J Soc Gynecol Investig   Vol. 13   page: 566-572   2006

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  70. Hypoxic ischemic encephalopathy associated with neonatal seizures without other neurological abnormalities. Reviewed

    Sato Y, Okumura A, Kato T, Hayakawa F, Kuno K, Watanabe K.

    Brain Dev   Vol. 25   page: 215-219   2003

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  71. Paternal UPD14 is responsible for a distinctive malformation complex. Reviewed

    Kurosawa K, Sasaki H, Sato Y, Yamanaka M, Shimizu M, Ito Y, Okuyama T, Matsuo M, Imaizumi K, Kuroki Y, Nishimura G.

    Am J Med Genet   Vol. 110   page: 268-272   2002

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  72. Hypocarbia in preterm infants with periventricular leukomalacia: the relation between hypocarbia and mechanical ventilation. Reviewed

    Okumura A, Hayakawa F, Kato T, Itomi K, Maruyama K, Ishihara N, Kubota T, Suzuki M, Sato Y, Kuno K, Watanabe K.

    Pediatrics   Vol. 107   page: 469-475   2001

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▼display all

MISC 5

  1. Muse細胞製品(CL2020)を用いた新生児低酸素性虚血脳症に対する医師主導治験 Invited

    佐藤義朗

    愛知県小児科医会会報   Vol. 113   page: 3 - 8   2021.6

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    Authorship:Lead author, Corresponding author   Language:Japanese   Publisher:愛知県小児科医会  

  2. Muse細胞を用いた新生児低酸素性虚血性脳症の新規治療法開発~動物実験から探索的臨床試験(医師主導治験) まで~ Invited

    佐藤義朗

    日本周産期・新生児医学会雑誌   Vol. 56 ( 4 ) page: 1194 - 1197   2021.4

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    Authorship:Lead author, Corresponding author   Language:Japanese  

  3. 低酸素性虚血性脳症治療薬 Invited

    神澤孝洋 佐藤義朗

    周産期医学   Vol. 50   page: 366 - 369   2021.3

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    Language:Japanese  

  4. 周産期脳障害に対する幹細胞療法 Invited

    佐藤義朗

    日本新生児成育医学会雑誌   Vol. 33 ( 1 ) page: 41 - 46   2021.2

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    Authorship:Lead author, Corresponding author   Language:Japanese  

  5. 臍帯血幹細胞による細胞療法・再生医療 Invited

    上田一仁 佐藤義朗 早川昌弘

    臨床婦人科産科   Vol. 74 ( 10 ) page: 1062 - 1067   2021.1

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Presentations 18

  1. Development of novel therapies for bronchopulmonary dysplasia and perinatal brain injury using multilineage-differentiating stress-enduring cells, as regenerative treatments Invited International conference

    Yoshiaki Sato

    The PAS 2021 Virtual Meeting   2021.5.2 

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    Event date: 2021.4 - 2021.5

    Language:English   Presentation type:Symposium, workshop panel (public)  

    Country:United States  

  2. 新生児低酸素性虚血性脳症に対する幹細胞療法の開発~動物実験から臨床試験まで~ Invited

    佐藤義朗

    第124回日本小児科学会学術集会  2021.4.16 

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    Event date: 2021.4

    Language:Japanese   Presentation type:Symposium, workshop panel (public)  

  3. 胎児発育不全に伴う脳発達異常の早期診断法確立に向けたバイオマーカーの探索

    小野田淳人、北瀬悠磨、辻雅弘、上田一仁、高橋義行、早川昌弘、佐藤義朗

    第124回日本小児科学会学術集会  2021.4.16 

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    Event date: 2021.4

    Language:Japanese   Presentation type:Oral presentation (general)  

  4. Fetal growth restriction induces glucose intolerance and impaired islet morphology in catch-up growth in male rats

    2021.4.16 

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    Event date: 2021.4

    Language:English   Presentation type:Oral presentation (general)  

  5. Development of novel stem cell therapies for perinatal brain injury Invited

    Yoshiaki Sato

    2021.3.28 

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    Event date: 2021.3

    Language:English   Presentation type:Symposium, workshop panel (public)  

  6. 修復幹細胞(Muse細胞)を用いた低酸素性虚血性脳症に対する新規治療法開発 Invited

    佐藤義朗

    第3回愛知県新生児医療スキルアップ研修会  2021.3.14 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Public lecture, seminar, tutorial, course, or other speech  

  7. 胎児発育不全に伴う脳発達異常の新規治療法確立に向けた基礎研究:Muse細胞による改善効果

    小野田淳人、北瀬悠磨、辻雅弘、上田一仁、早川昌弘、佐藤義朗

    第20回日本再生医療学会総会  2021.3.11 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Oral presentation (general)  

  8.  Muse細胞を用いた周産期脳障害の新規治療法開発~探索的臨床試験(医師主導治験)~

    佐藤義朗

    革新的医療技術創出拠点令和二年度成果報告会  2021.3.4 

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    Event date: 2021.3

    Language:Japanese   Presentation type:Poster presentation  

  9. 新生児の肺障害を修復する多能性幹細胞(Muse細胞)を用いた再生治療の開発 Invited

    佐藤義朗、清水忍

    令和2年度 AMED 再生・細胞医療・遺伝子治療研究開発交流会  2021.1.19 

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    Event date: 2021.1

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  10. 周産期脳障害に対する幹細胞療法の開発~脳性麻痺撲滅を目指して~ Invited

    佐藤義朗

    第31回日本小児整形外科学会学術集会  2020.12.3 

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    Event date: 2020.12

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  11. SHIELD試験の立ち上げ:低体温療法を実施した新生児低酸素性虚血性脳症に対するCL2020の安全性及び忍容性を検討する用量漸増臨床試験

    松山奈央、清水忍、片山朱美、平川晃弘、安藤昌彦、上田一仁、鈴木俊彦、佐藤義朗

    第41回日本臨床薬理学会学術総会  2020.12.3 

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    Event date: 2020.12 - 2020.1

    Language:Japanese   Presentation type:Poster presentation  

  12. Muse細胞を用いた新生児低酸素性虚血性脳症の新規治療法開発~動物実験から探索的臨床試験(医師主導治験)まで~ Invited

    佐藤義朗

    第56回日本周産期・新生児医学会学術集会  2020.11.28 

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    Event date: 2020.11 - 2020.12

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  13. 胎児発育不全(胎児慢性低灌流)による脳障害に対する幹細胞療法 Invited

    佐藤義朗

    超早産児神経発達症研究会   2020.8.2 

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    Event date: 2020.8

    Language:Japanese   Presentation type:Oral presentation (invited, special)  

  14. Identification of biomarkers for early prediction of abnormal brain development following fetal growth restriction

    第43回日本神経科学大会  2020.7.29 

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    Event date: 2020.7 - 2020.8

    Language:English  

  15. Muse細胞を用いた新生児低酸素性虚血性脳症の新規治療法開発~探索的臨床試験(医師主導治験)~

    佐藤義朗 上田一仁 清水忍 鈴木俊彦 呉尚治 三浦良介 新井紗記子 橋本佑樹 本部和也 青山藍子 谷口顕信 棚橋義浩 伊藤美春 齊藤明子 村松友佳子 城所博之 早川昌弘

    第41回日本炎症・再生医学会  2020.7.8 

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    Event date: 2020.7

    Language:Japanese   Presentation type:Poster presentation  

  16. Muse細胞を用いた新生児低酸素性虚血性脳症に対する新規治療法の開発 Invited

    佐藤義朗

    第19回日本再生医療学会総会  2020.5.18 

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    Event date: 2020.5

    Language:Japanese   Presentation type:Symposium, workshop panel (nominated)  

  17. 新生児低酸素性虚血性脳症と低体温療法に対して、免疫不全(SCID)マウスはどのような脳障害を呈するのか?

    辻雅弘、田中えみ、佐藤義朗、小川優子 

    第124回日本小児科学会学術集会  2020.4.16 

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    Event date: 2020.4

    Language:Japanese   Presentation type:Oral presentation (general)  

  18. 新生児モデルを用いたドップラーセンサによる心拍測定実験

    上林真司 佐藤里咲 坂口彰浩 佐藤菜津子 佐藤義朗

    2020年電子情報通信学会総合大会  2020.3.17 

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    Event date: 2020.3

    Language:Japanese  

▼display all

Research Project for Joint Research, Competitive Funding, etc. 9

  1. ゼラチン繊維基材を用いた脂肪幹細胞による横隔膜再生

    2021.4 - 2022.3

    国立研究開発法人日本医療研究開発機構  革新的医療技術創出拠点プロジェクト橋渡し研究戦略的推進プログラム シーズA 

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    Authorship:Principal investigator  Grant type:Competitive

  2. Muse細胞を用いた周産期脳障害の新規治療法開発 探索的臨床試験(医師主導治験)

    2020.8 - 2022.3

    国立研究開発法人日本医療研究開発機構  革新的医療技術創出拠点プロジェクト橋渡し研究戦略的推進プログラム シーズC 

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    Authorship:Principal investigator  Grant type:Competitive

  3. 新生児の肺障害を修復する多能性幹細胞(Muse細胞)を用いた再生治療の開発

    2020.6 - 2023.3

    国立研究開発法人日本医療研究開発機構  再生医療実現拠点ネットワークプログラム 

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    Authorship:Principal investigator  Grant type:Competitive

  4. 新生児慢性肺疾患の治療薬開発

    2020.4 - 2021.3

    国立研究開発法人日本医療研究開発機構  革新的医療技術創出拠点プロジェクト橋渡し研究戦略的推進プログラム シーズA 

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    Authorship:Principal investigator  Grant type:Competitive

  5. 脱脂肪幹細胞を用いた新生児壊死性腸炎に対する新規治療法の開発

    2018.11 - 2019.3

    国立研究開発法人日本医療研究開発機構  革新的医療技術創出拠点プロジェクト橋渡し研究戦略的推進プログラム シーズA 

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    Authorship:Principal investigator  Grant type:Competitive

  6. 幹細胞を用いた新生児壊死性腸炎に対する新規治療法の開発

    2018.4 - 2019.3

    国立研究開発法人日本医療研究開発機構  革新的医療技術創出拠点プロジェクト橋渡し研究戦略的推進プログラム シーズA 

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    Authorship:Principal investigator  Grant type:Competitive

  7. Muse細胞を用いた周産期脳障害の新規治療法開発

    2017.4 - 2019.3

    国立研究開発法人日本医療研究開発機構  革新的医療技術創出拠点プロジェクト橋渡し研究戦略的推進プログラム シーズB 

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    Authorship:Principal investigator  Grant type:Competitive

  8. 幹細胞を用いた胎児発育不全による脳障害に対する新規治療法の開発

    2017.4 - 2018.3

    国立研究開発法人日本医療研究開発機構  革新的医療技術創出拠点プロジェクト橋渡し研究戦略的推進プログラム シーズA 

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    Authorship:Principal investigator  Grant type:Competitive

  9. Development a novel regenerative treatment for perinatal brain injury with Muse cells

    2016.4 - 2017.3

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    Grant type:Competitive

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KAKENHI (Grants-in-Aid for Scientific Research) 12

  1. 周産期脳障害に対する脱分化脂肪細胞を用いた新規治療開発

    2014.4 - 2017.3

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

  2. 小脳障害に着目した早産児の神経学的障害の病態解明と幹細胞療法の治療機序解明

    Grant number:21K07794  2021.4 - 2024.3

    佐藤 義朗

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    Authorship:Principal investigator 

    Grant amount:\4160000 ( Direct Cost: \3200000 、 Indirect Cost:\960000 )

  3. 肺高血圧症の閉塞性病変における平滑筋細胞形質変換のプロテオームによる網羅的解析

    Grant number:20K08155  2020.4 - 2023.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    加藤 太一

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    Authorship:Coinvestigator(s) 

    肺高血圧症は、肺動脈が進行性に狭窄、閉塞する難治性疾患であり、重篤な場合、生命予後が不良な疾患です。この研究は実験的に作成した肺高血圧モデルにおいて、血管が閉塞している部分と閉塞していない部分のそれぞれから蛋白質を抽出し、プロテオーム解析という手法で、両者で異なる蛋白質すなわち閉塞に関連する蛋白質を同定し、その機能を検証することで、病気のメカニズムを解明することを目的としています。

  4. Elucidation of severe mechanism and development of novel therapy against chronic lung disease in SGA infants caused by pregnancy induced hypertension

    Grant number:19K08341  2019.4 - 2022.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

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    Authorship:Coinvestigator(s) 

  5. "再生”を用いた新生児/小児肺高血圧症に対する新規治療法の開発

    Grant number:19K08250  2019.4 - 2022.3

    日本学術振興会  科学研究費助成事業 基盤研究(C)  基盤研究(C)

    齊藤 明子

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    Authorship:Coinvestigator(s) 

    新生児/小児の肺高血圧症(PAH)は、予後不良で難治の疾患である。
    PAHに伴う心拍出量低下や右心不全は患者のQOLを著しく低下させ、死亡率は依然として高い。
    現状では複数の薬剤を併用投与するが、その効果には限界があり、PAHの患者に見られる広範な微小血管床の喪失には無効である。
    PAHの新たな治療方策のひとつとして、再生/細胞療法が挙げられる。
    本研究では、A細胞がPAHにおいて有効であることを示し、新規治療につなげるための基礎研究を行う。

  6. Development of a novel two-step cell therapy for perinatal hypoxic ischemic encephalopathy

    Grant number:17K10175  2017.4 - 2020.3

    Sato Yoshiaki

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    Authorship:Principal investigator 

    Grant amount:\4680000 ( Direct Cost: \3600000 、 Indirect Cost:\1080000 )

    The purpose of this study was to develop a novel therapy for perinatal HIE using allogenic and autologous UMSCs.
    In the present study, we established a method to obtain homogeneous UCMSCs from rat umbilical cords. The intravenously administered UCMSCs were engrafted in the brain, lungs, and liver.
    However, immunohistological evaluation after acute phase treatment did not show the treatment effect of UCMSC. In the behavioral evaluation after chronic treatment, some behavioral abnormalities were observed in HIE model rats, but no treatment effect was confirmed by UCMSC administration.

  7. Development of a novel treatment for neonatal chronic lung disease by stem cells

    Grant number:16K19683  2016.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Young Scientists (B)  Grant-in-Aid for Young Scientists (B)

    Saito Akiko, Sato Yoshiaki, Suzuki Toshihiko

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    Neonatal chronic lung disease (CLD) is still an important complication in very low birth weight infants (VLBW). Stem cell therapy using various cell sources has been recently identified as a novel therapy for CLD. Multilineage-differentiating stress-enduring (Muse) cells are a novel type of endogenous stem cells that are able to self-renew, display pluripotency. The purpose of this study was to evaluate the treatment effect of Muse cells on CLD using a rat model.
    In this study, we have shown that (1) intravenously injected Muse cells migrated into injured lung in CLD model rats, and the number of them was higher than that of non-Muse Mesenchymal Stem Cells (MSCs). (2) Muse cells injected intravenously ameliorated impaired alveolar growth induced by hyperoxia in the rat model, and the beneficial effect was much more evident than that done by non-Muse MSCs. (3) Muse cells, but not non-Muse cells, ameliorated impaired pulmonary hypertension in the CLD model.

  8. The development of cell therapies for complications in several organs in infants with CLD

    Grant number:16K15534  2016.4 - 2019.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Challenging Exploratory Research  Grant-in-Aid for Challenging Exploratory Research

    Hayakawa Masahiro

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    Authorship:Coinvestigator(s) 

    Neonatal chronic lung disease is a risk factor for not only respiratory disorder but also psychomotor developmental delay. And it is well known that that it may affect various organs other than respiratory organs. In this study, we investigated the effects of multiple organs to administer 7ND-MSC and Multilineage-differentiating stress enduring cells (Muse cells). Intravenous administration of 7ND-MSC and Muse cells suppresses systemic inflammation and airway inflammation more strongly than normal mesenchymal stem cells, and is effective in treating alveolar developmental disorders in lung tissue and pulmonary hypertensive changes in the heart.

  9. Development of novel therapy for chronic lung disease associated pulmonary arterial hypertension via proliferation of pulmonary alveoli.

    Grant number:15K09687  2015.4 - 2018.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    KATO TAICHI

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid) 

    In this study, we studied whether EGF promoted the proliferation of pulmonary alveoli and improved pulmonary hypertension using hyperoxia induced chronic lung disease rat neonate model. The time course study on the duration of hyperoxia revealed that compared to control rats, systolic right ventricular pressure (RVP) and the percentage of muscularized pulmonary vessels increased significantly in the rats after 14 days of hyperoxia challenge. Daily administration of EGF slightly decreased RVP and the percentage of muscularized pulmonary vessels and slightly improved the structure of pulmonary alveoli. Furthermore, hyperoxia decreased the expression of BMPR2, FGFR1 and MMP14 in the lung, which was not restored by EGF. These results showed chronic hyperoxia for 14 days caused chronic lung disease associated pulmonary hypertension in rat neonates. EGF administration showed only slight improvement of pulmonary hypertension and the structure of pulmonary alveoli in this model.

  10. Stem cell therapy for brain injuries of infants with fetal growth restriction

    Grant number:26293252  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (B)  Grant-in-Aid for Scientific Research (B)

    Hayakawa Masahiro

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    Fetal growth retardation is the cause of small-for-gestational age (SGA) and is a high-risk group of neurological post-injury. Various therapeutic interventions have been done to prevent post-neurological impairment, but none of them have been effective. In this study, SGA rats were inferior in early behavioral evaluation of neonates, and immune-histological analysis showed that nerve cells decreased. Intravenous administration of umbilical cord blood after birth improved behavior early in the neonatal period and also significantly increased neuronal cells immunologically. This suggested the possibility of novel therapy for neurological development of children with SGA.

  11. Development of a novel therapy for perinatal brain injury with dedifferentiated fat cells

    Grant number:26461630  2014.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    Sato Yoshiaki, MATSUMOTO TARO, TSUHI MASAHIRO

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    In the present study, we aimed to determine whether the outcome of HIE can be improved by DFAT cell treatment. 24 h after HI insult, DFAT cells were injected at 10^5 cells/pup into the right external jugular vein. The number of cells that stained positive for active caspase-3, ED-1, and 4-HNE decreased in the DFAT-treated group. The HI insult led to a motor deficit according to the rotarod treadmill and cylinder test, where it significantly affected the vehicle group, whereas no difference was confirmed between the DFAT and sham groups. According to in vitro experiments, the cell death rates in the DFAT-CM-treated cells were significantly lower than those in the controls. In the DFAT-CM, several neurotrophic factors, IGF-1、NGF、NT3 were detected, and these factors were increased in the ipsilateral brain after injection of DFAT. Our results indicate that intravenous injection with DFAT cells is effective for ameliorating HI brain injury, possibly via paracrine effects.

  12. Transplantation of rat umbilical cord blood cells for neonatal hypoxic-ischemic brain injury.

    Grant number:25461659  2013.4 - 2017.3

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)  Grant-in-Aid for Scientific Research (C)

    NAKANISHI KEIKO, SATO YOSHIAKI, ITO MIHARU, HIGASHI YUJIRO, MIZUTANI YUKA, HIRAKAWA AKIHIRO

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    Mononuclear cells isolated from rat umbilical cord blood were expanded (stem cell enriched-umbilical cord blood cells; SCE-UCBCs) and they were administered intraperitoneally to neonatal hypoxic-ischemic encephalopathy (HIE) model of rats. Administration of SCE-UCBCs increased the residual brain volume and decreased the accumulation of activated microglia to lesion site. These results indicate that UCBCs could ameliorate HI injury, possibly through an endogenous response.

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Industrial property rights 9

  1. 低酸素性虚血性脳症の予防又は治療剤及びその利用

    佐藤義朗 齊藤明子

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    Applicant:名古屋大学

    Application no:2014-22411  Date applied:2014.2

    Country of applicant:Domestic  

  2. 神経細胞の細胞死抑制剤等

    大平敦彦、佐藤義朗、中西圭子、前田 浩

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    Applicant:愛知県と生化学工業株式会社

    Application no:2006-13981  Date applied:2006.5

    Announcement no:2007-30861 

    Country of applicant:Domestic  

  3. 脳損傷抑制剤

    大平敦彦、佐藤義朗、中西圭子、前田 浩

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    Applicant:愛知県と生化学工業株式会社

    Application no:2006-139843  Date applied:2006.5

    Patent/Registration no:5279491  Date registered:2013.5 

    Country of applicant:Domestic  

  4. 脳損傷改善剤

    大平敦彦、佐藤義朗、中西圭子、前田 浩

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    Applicant:愛知県と生化学工業株式会社

    Application no:PCT/JP2007/060388  Date applied:2007.5

    Country of applicant:Foreign country  

  5. 呼吸補助治療具

    伊藤 美春, 佐藤 義朗, 早川 昌弘, 高▼成 啓介, 亀井 讓

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    Applicant:国立大学法人名古屋大学

    Application no:特願2018-085877  Date applied:2018.4

    Announcement no:特開2019-187947  Date announced:2019.10

    J-GLOBAL

  6. 多能性幹細胞による慢性肺疾患の改善及び治療

    佐藤 義朗, 鈴木 俊彦, 清水 忍, 水野 正明, 早川 昌弘, 出澤 真理

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    Applicant:国立大学法人名古屋大学, 株式会社生命科学インスティテュート, 国立大学法人東北大学

    Application no:JP2017028323  Date applied:2017.8

    Announcement no:WO2018-025973  Date announced:2018.2

    J-GLOBAL

  7. 多能性幹細胞による周産期脳障害の改善及び治療

    佐藤 義朗, 鈴木 俊彦, 清水 忍, 水野 正明, 早川 昌弘, 出澤 真理

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    Applicant:国立大学法人名古屋大学, 株式会社生命科学インスティテュート

    Application no:JP2017018416  Date applied:2017.5

    Announcement no:WO2017-199976  Date announced:2017.11

    J-GLOBAL

  8. 精子活性化方法及びその用途

    山本 徳則, 鈴木 哲, 松川 宜久, 舟橋 康人, 佐藤 義朗, 後藤 百万, 村瀬 哲磨

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    Applicant:国立大学法人名古屋大学, 国立大学法人岐阜大学

    Application no:特願2013-222630  Date applied:2013.10

    Announcement no:特開2015-082987  Date announced:2015.4

    Patent/Registration no:特許第6241819号  Date issued:2017.11

    J-GLOBAL

  9. 精子活性化方法及びその用途

    山本 徳則, 鈴木 哲, 松川 宣久, 舟橋 康人, 佐藤 義朗, 後藤 百万, 村瀬 哲磨

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    Application no:2013-222630  Date applied:2011.10

    Country of applicant:Domestic  

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