Language：English   Publishing type：Research paper (scientific journal)  

PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1097

Language：English   Publishing type：Research paper (scientific journal)  

OBJECTIVES:
The effect of growth differentiation factor 5 and bone morphogenetic protein 2 on human periodontal ligament-derived cells was investigated with special reference to tendo/ligamentogenesis-related markers.
MATERIALS AND METHODS:
Effects of each factor were analyzed by quantitative PCR for scleraxis and tenomodulin and by western blotting for scleraxis. After exposure to those factors, STRO-1-positive and STRO-1-negative fractions of human periodontal ligament tissues were isolated with an immunomagnetic cell sorting system, and the expression of scleraxis in each fraction was analyzed by western blotting. Non-separated crude cells were used as a control.
RESULTS:
Growth differentiation factor 5 and bone morphogenetic protein 2 did not increase alkaline phosphatase activity in crude periodontal ligament-derived cells. Growth differentiation factor 5, but not bone morphogenetic protein 2, increased the expression of scleraxis in crude, STRO-1-positive and STRO-1-negative periodontal ligament-derived cells. The expression of scleraxis in STRO-1-positive periodontal ligament-derived cells was significantly less compared to that in crude P2 and STRO-1-negative periodontal ligament-derived cells.
CONCLUSION:
Growth differentiation factor 5 induced the expression of scleraxis and may enhance tendo/ligamentogenesis in human periodontal ligament-derived cells. The expression of scleraxis was higher in STRO-1-negative fraction, suggesting more differentiated state of the cells.

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

Gene expression profiles in normal human gingival and dermal fibroblasts were investigated using DNA microarrays. Their fundamental characteristics were almost identical, but 5% of their genes were uniquely expressed. These results help us to choose an optimal cell source for effective fibroblast-based cell therapy that is dependent on differential gene expression profiles.

Language：English   Publishing type：Research paper (scientific journal)  

In this research, we examined the effect on wound healing applying basic fibroblast growth factor (b-FGF) that is approved for clinical use to enhance wound healing and human deciduous teeth dental pulp cells (hDPCs) in clinics, but that have been attracting attention as a novel stem cell source in recent years. Human deciduous teeth were harvested from healthy volunteers, and hDPCs were isolated. We used a nude mouse full-thickness skin defect model and evaluated wound healing by macroscopic view and histologic and histomorphometric analysis. The mice were randomly divided into 4 groups: phosphate-buffered saline-treated group (control group), b-FGF-treated group (b-FGF group), hDPC-treated group (hDPC group), and hDPC and b-FGF-treated group (hDPC/b-FGF group). Basic fibroblast growth factor and hDPC groups accelerated wound healing compared with the control group. There was no statistically significant difference in wound healing observed between the hDPC and b-FGF groups. The hDPC/b-FGF group demonstrated accelerated wound healing compared with other groups. At day 14, PKH26-positive cells were surrounded by human type I collagen in hDPC and hDPC/b-FGF groups in immunohistologic evaluation. Significantly increased collagen fibril areas in wound tissues were observed in b-FGF, hDPC, and hDPC/b-FGF groups as compared with the control group at days 7 and 14. Our results showed that the hDPC/b-FGF group significantly promotes wound healing compared with other groups. This study implies that deciduous teeth that are currently considered as medical spare parts might offer a unique stem cell resource for potential of new cell therapies for wound healing in combination with b-FGF.

Language：English   Publishing type：Research paper (scientific journal)  

Background Aims: In recent years, stem cells from human exfoliated deciduous teeth (SHED) have received attention as a novel stem cell source with multipotent potential. We examined the effect on wound-healing promotion with unique stem cells from deciduous teeth as a medical waste.
Methods: An excisional wound-splinting mouse model was used and the effect of wound healing among SHED, human mesenchymal stromal cells (hMSCs), human fibroblasts (hFibro) and a control (phosphate-buffered saline; PBS) was evaluated by macroscopy, histology and enzyme-linked immunosorbent assay (ELISA), and the expression of hyaluronan (HA), which is related to wound healing, investigated.
Results: SHED and hMSCs accelerated wound healing compared with hFibro and the control. There was a statistically significant difference in wound healing area among hFibro, hMSCs and SHED compared with the control after day 5. At days 7 and 14 after cell transplantation, the histologic observation showed that transplanted PKH26-positive cells were surrounded by human HA binding protein, especially in hMSCs and SHED. HA expression volume values were 1558.41 ± 60.33 (control), 2092.75 ± 42.56 (hFibro), 2342.07 ± 188.10 (hMSCs) and 2314.85 ± 164.91 (SHED) ng/mg, respectively, and significantly higher in hMSCs and SHED compared with hFibro and control at days 7 and 14 (P < 0.05).
Conclusions: Our results show that SHED hMSCs have similar effects of wound-healing promotion as hFibro and controls. This implies that SHED might offer a unique stem cell resource and the possibility of novel cell therapies for wound healing in the future.

Language：English   Publishing type：Research paper (scientific journal)  

Background: The techniques to isolate and purify retinal pigment epithelial (RPE) cells from small piece of autologous tissues are extremely difficult, and it is important to develop an efficient cell culture technique for RPE cells. The purpose of this study was to investigate the effect of 3T3-J2 cells and conditioned medium from 3T3-J2 cells on the proliferation of cultured RPE cells.
Methods: RPE cells from pigmented rabbits and a human RPE-derived cell line, ARPE-19, were used. First, the effects of co-culturing RPE cells with 3T3-J2 cells on the growth of the cells were analyzed. Second, the effects of the conditioned medium from 3T3-J2 cells on the proliferation of both types of cells were investigated. And third, the effects of the conditioned medium on RPE cell culture from a surgically removed choroidal neovascular (CNV) membrane were investigated.
Results: The 3T3-J2 cells increased the proliferation of both rabbit RPE cells and ARPE-19 cells. The number of rabbit RPE cells cultured in a mixture of the conditioned medium from 3T3-J2 cells was significantly higher than that in the reported optimal condition, and a similar tendency was observed for ARPE-19 cells. The results from enzyme-linked immunosorbent assay showed the presence of PDGF-AB, VEGF and IGF-I in the conditioned medium. The conditioned medium also promoted selective growth of human RPE cells from CNV.
Discussion: The results from this study present the conditions for efficient and selective culture of primary RPE cells.

Language：English   Publishing type：Research paper (scientific journal)  

We reconstructed four knee and lower leg defects using the sural artery perforator flap between 2000 and 2003, and describe them here. The sural artery perforator flap can save the gastrocnemius muscle, its motor nerve, deep fascia, lesser saphenous vein, and sural nerve with no functional loss. Intramuscular dissection of the perforator achieves increased length of the pedicle compared with a conventional gastrocnemius myocutaneous flap. The flap is thin, and either the medial or lateral sural artery may be used. The flap is suitable in selected cases for regional reconstruction around the knee and upper half of the lower leg as a pedicled flap.

Authorship：Lead author   Language：English   Publishing type：Research paper (scientific journal)  

In clinical studies and animal models, low-intensity ultrasound (US) promotes fracture repair and increases mechanical strength. US also promotes cartilage healing by increasing glycosaminoglycan synthesis of chondrocytes. As mesenchymal stem cells (MSCs) have the ability to differentiate into chondrocytes, US may promote their differentiation. Here, we evaluated the effects of US on the differentiation of MSCs toward chondrocytes and cartilage matrix formation. When human MSCs cultured in pellets were treated with transforming growth factor beta (TGF-beta, 10 ng/mL), they differentiated into chondrocytes as assessed by alcian blue staining and immunostaining for aggrecan, but nontreated cell pellets did not. Furthermore, when low-intensity US was applied for 20 min every day to the TGF-beta-treated cell pellets, chondrocyte differentiation was enhanced. Biochemically, aggrecan deposition was increased by 2.9- and 8.7-fold by treatment with TGF-beta alone, and with both TGF-beta and US, respectively. In contrast, cell proliferation and total protein amount appeared unaffected by these treatments. These results indicate that low-intensity US enhances TGF-beta-mediated chondrocyte differentiation of MSCs in pellet culture and that application of US may facilitate larger preparations of chondrocytes and the formation of mature cartilage tissue.

Language：English  

DOI： 10.1021/acsbiomaterials.2c01141

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PubMed

Language：English  

DOI： 10.1097/PRS.0000000000009312

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PubMed

Language：Japanese  

DOI： 10.1155/2021/5586523

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PubMed

Language：Japanese  

DOI： 10.1097/GOX.0000000000003525

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PubMed

Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.bjps.2020.08.060

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PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1097/GOX.0000000000002872

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PubMed

Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： 10.18999/nagjms.82.2.291

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PubMed

Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： 10.1055/s-0038-1676777

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PubMed

Language：Japanese  

DOI： 10.1055/s-0039-1692394

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PubMed

Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： 10.18999/nagjms.82.1.123

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PubMed

Language：Japanese   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.bjps.2018.12.027

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PubMed

Language：English   Publishing type：Research paper (scientific journal)  

DOI： 10.1016/j.jpra.2019.01.003

PubMed

Authorship：Lead author   Language：Japanese  

Language：English   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Authorship：Lead author   Language：Japanese  

しわや軽度の瘢痕などは、本人にとってはQuality of Lifeに関わる問題となり、多くの人が治療を受けている。各種の治療法があるものの、効果と侵襲のバランスを考えると、治療の選択が難しい。そのため低侵襲かつ持続的効果の期待できる方法として、再生医療に注目が集まっている。1990年代より患者本人の耳介後面皮膚から得た線維芽細胞を牛血清添加培地にて培養・増殖させた後、患部に注入するという治療が行われている。我々は細胞源として歯肉由来線維芽細胞を使用し、またBSEなどのリスクを回避するため自己血清を用いて培養し、しわや瘢痕の治療に使用している。本稿では、線維芽細胞の採取から作製、注入法などについて紹介し、症例写真を供覧して本法の効果やその限界などに関しても記載している。また本法に関する法的環境や問題点についても触れた。さらに新たに始まっている他家培養線維芽細胞移植も紹介した。

Authorship：Lead author   Language：Japanese  

再生医療は機能障害に陥った組織を、その個体のもつ再生能力を引き出し、機能再生を行う医療である。その手法は比較的低侵襲かつ自己細胞を使用することから、新たな治療法として期待されている。老年医学領域における再生医療は広範多岐にわたるが、本稿ではその具体例の一部として、国内で行われている5例(脳、心筋、軟骨、歯槽骨、皮膚)を紹介する。また再生医療領域での最近の話題であるES細胞やiPS細胞に関しても言及する。

Authorship：Lead author   Language：Japanese  

Regenerative medicine refers to research and treatments that restore damaged tissues. It consists of three factors: cells, chemical substances, and scaffolds. One of its purposes is rejuvenation, and from this point of view, it really resembles anti-aging medicine. Recently, studies concerning stem cells such as embryonic stem cells (ES) and induce pluripotent stem cells (iPS) have made remarkable progress and seem promising, but are still far from clinical use. In contrast, we introduce a few examples of anti-aging medicine using regenerative medicine methods that have been applied successfully in clinical use such as cultured fibroblast, adipose tissue derived stem cells and platelet rich plasma (PRP), and continue researching cultured dermal papilla cells.

Authorship：Lead author   Language：Japanese  

Authorship：Lead author   Language：English  

Two types of cell therapy for facial anti-aging in my clinical experience are introduced in this presentation. One therapy is cultured gingival fibroblasts injection. This procedure lasts for at least one year, making it a good option for patients. The other is platelet rich plasma injection. The results of the preliminary data are promising, but not yet well understood. More clinical data and long-term follow-up is needed.

Authorship：Lead author   Language：Japanese  

自己培養線維芽細胞を皮内に注入し、陥凹瘢痕を改善する。

Authorship：Lead author   Language：Japanese  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese  

Language：Japanese   Publishing type：Research paper (scientific journal)  

Language：Japanese

Event date： 2013.10

Language：Japanese   Presentation type：Oral presentation (general)  

Venue：東京国際フォーラム   Country：Japan  

2002年Rassmanらは、ドナー瘢痕を分散させ目立たなくする目的で小口径パンチにて毛根単位を採取するFollicular unit extraction（以下FUE）法を報告した。本法は、線状瘢痕を残さない、採取部が限定されない、患部疼痛がstrip法と比較し明らかに少ないなどの利点から世界中に普及しつつあり、国際毛髪外科学会でも植毛患者の4割ほどが本法による植毛術を受けているとの報告がなされた。しかし、毛根採取に当たっては、ドナー部毛髪を１mm程度にしなければならず、術後剃毛部位を被覆できないこともあり、本術式を選択したい患者にとっては大きな問題となっていた。今回われわれは、後頭部ドナーに部分ヘアピースを使用することにより良好な結果を得たので報告する。
方法：FUE法を行った患者に対し、後頭部ドナー被覆目的でヘアピースを使用した。本製品は、メッシュ上に人工毛を接着したもので、カラーバリエーションがあり、カールにも対応できるヘアピースである。本製品の上端を自毛と編み込み、さらに医療用接着剤で毛髪と接着させて固定する。
結果：術直後より、後頭部を被覆することが可能であった。ドナー領域内の採取されていない毛髪は伸びていくため、剃毛部位としていない部位との差が無くなり、術後１ヶ月程度の使用でヘアピースを外すことが可能であった。また経過観察中、本製品使用によるトラブルは認めなかった。本法は広範囲の剃毛を要する多数の移植が必要な患者、後頭部毛髪密度の低い患者や社会的に手術を隠したい患者などには特に良い適応であると考えた。
まとめ：FUE法ドナー部へヘアピースを使用することは、後頭部ドナーを被覆するのに有用であり、簡便な方法にも拘わらずドナー露出という問題点を解決する事により、本術式の普及に貢献すると考えた。われわれの渉猟した限りでは本法に関する報告が無く、今回報告することとした。

Event date： 2013.1

Language：English   Presentation type：Poster presentation  

Country：Japan  

Platelet-rich plasma (PRP) has 2 to 6 times the amount of platelets than human blood. It contains many growth factors, such as PDGF, TGF-beta, IGF and so on. Therefore, PRP is utilized in many clinical applications. Since Dr. Kubota presented regeneration of facial aging by platelet-rich plasma at the Japanese Society of Aesthetic Plastic Surgery in 2006, it is now more popular in Japan. In this presentation, we introduce our PRP injection method. 20-mL of patient blood is taken into the two PRP preparation kit tubes, and centrifuged at 1,200 g for 8 minutes. 2-mL of plasma is collected as PRP. After a nerve block and/or lidocaine cream, we make stab incisions on the wrinkles or lines, and then bluntly dissect just underneath them using small diameter of 18G-lipoinjection cannula. We display the result of our case in this poster.

Event date： 2010.7

Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

Venue：神戸   Country：Japan  

Event date： 2009.9

Language：English   Presentation type：Poster presentation  

Venue：Seoul   Country：Korea, Republic of  

Event date： 2009.5

Language：Japanese   Presentation type：Symposium, workshop panel (nominated)  

Venue：ホテル日航東京   Country：Japan  

Event date： 2009.4

Language：English   Presentation type：Oral presentation (general)  

Country：Korea, Republic of  

Event date： 2008.11

Language：English   Presentation type：Oral presentation (general)  

Event date： 2008.7

Language：English   Presentation type：Oral presentation (invited, special)  

Country：Malaysia  

Event date： 2007.7

Language：Japanese   Presentation type：Poster presentation  

Venue：国立京都国際会館   Country：Japan  

Event date： 2007.4

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2007.3

Language：English   Presentation type：Oral presentation (invited, special)  

Event date： 2006.12

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2006.11

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2006.5

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2006.5

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2005.10

Language：English   Presentation type：Poster presentation  

Event date： 2005.9

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2005.4

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2004.10

Language：Japanese   Presentation type：Oral presentation (general)  

Country：Japan  

Event date： 2004.10

Language：English   Presentation type：Poster presentation  

Event date： 2004.1

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2003.3

Language：Japanese   Presentation type：Oral presentation (invited, special)  

Country：Japan  

Event date： 2002.10

Language：English   Presentation type：Poster presentation  

Applicant：Rader Fisherman & Grauer PLLC

Application no：US Patent application 20040197908  Date applied：2004

Country of applicant：Foreign country  

Applicant：帝人株式会社　上田実

Application no：特許出願2003-98989  Date applied：2003.4

Announcement no：特許公開2004-307350 

Date registered：2004.11 

Country of applicant：Domestic