Updated on 2025/03/27

写真a

 
SUZUKI, Hiromi
 
Organization
Research Institute of Environmental Medicine Division of Stress Adaptation and Protection Assistant Professor
Graduate School
Graduate School of Medicine
Title
Assistant Professor
Contact information
メールアドレス
External link

Degree 2

  1. 医学博士 ( 2003.3   藤田保健衛生大学 ) 

  2. 衛生学士 ( 1990.3   藤田保健衛生大学 ) 

Research Interests 3

  1. Imaging

  2. DDS

  3. microglia

Research Areas 1

  1. Others / Others  / 神経化学 神経病理学

Current Research Project and SDGs 9

  1. LMD-LC-MSによる脳内の薬物動態と神経伝達物質変化の細胞毎イメージング

  2. ミクログリア機能を反映するPETイメージング

  3. MALDI MSイメージングでの生体高分子の検出やLC-MS/MS分析でも質量イメージングを可能にする技術の確立

  4. 脳特異的ドラッグデリバリシステムの構築、微小血管解析システムを用いた脳・神経系ヘの細胞浸潤のイメージング

  5. 脳標的化ペプチドを用いた脳疾患のPET診断用システム開発

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Research History 15

  1. Nagoya University   Graduate School of Medicine   Assistant Professor

    2022.1

  2. Nagoya University   Assistant Professor

    2009.3

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    Country:Japan

  3. 株式会社ティッシュターゲティングジャパン    研究開発部   主任研究員

    2005.3 - 2009.2

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    Country:Japan

  4. OTAGO univ.   Department of Physiology   Researcher

    2003.5 - 2003.6

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    Country:New Zealand

  5. 藤田保健衛生大学(藤田医科大学)   放射線科   客員助教

    2019.4

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Education 2

  1. Fujita Health University

    - 2003.3

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    Country: Japan

  2. Fujita Health University

    1986.4 - 1990.3

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    Country: Japan

Professional Memberships 4

  1. 日本分子イメージング学会

  2. 日本バイオイメージング学会

  3. 日本神経化学会

  4. The Molecular Biology Society of Japan

 

Papers 84

  1. LC-MS/MS imaging with thermal film-based laser microdissection Reviewed International coauthorship International journal

    Michiko Oya, Hiromi Suzuki, Andrea Roxanne J. Anas, Koichi Oishi, Kenji Ono, Shun Yamaguchi, Megumi Eguchi, Makoto Sawada

    Analytical and Bioanalytical Chemistry   Vol. 410 ( 2 ) page: 491 - 499   2018.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Springer Verlag  

    Mass spectrometry (MS) imaging is a useful tool for direct and simultaneous visualization of specific molecules. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is used to evaluate the abundance of molecules in tissues using sample homogenates. To date, however, LC-MS/MS has not been utilized as an imaging tool because spatial information is lost during sample preparation. Here we report a new approach for LC-MS/MS imaging using a thermal film-based laser microdissection (LMD) technique. To isolate tissue spots, our LMD system uses a 808-nm near infrared laser, the diameter of which can be freely changed from 2.7 to 500 μm
    for imaging purposes in this study, the diameter was fixed at 40 μm, allowing acquisition of LC-MS/MS images at a 40-μm resolution. The isolated spots are arranged on a thermal film at 4.5-mm intervals, corresponding to the well spacing on a 384-well plate. Each tissue spot is handled on the film in such a manner as to maintain its spatial information, allowing it to be extracted separately in its individual well. Using analytical LC-MS/MS in combination with the spatial information of each sample, we can reconstruct LC-MS/MS images. With this imaging technique, we successfully obtained the distributions of pilocarpine, glutamate, γ-aminobutyric acid, acetylcholine, and choline in a cross-section of mouse hippocampus. The protocol we established in this study is applicable to revealing the neurochemistry of pilocarpine model of epilepsy. Our system has a wide range of uses in fields such as biology, pharmacology, pathology, and neuroscience. [Figure not available: see fulltext.].

    DOI: 10.1007/s00216-017-0739-2

    Web of Science

    Scopus

    PubMed

  2. Signal Sequence-Dependent Orientation of Signal Peptide Fragments to Exosomes. Reviewed

    Ono K, Niwa M, Suzuki H, Kobayashi NB, Yoshida T, Sawada M

    International Journal of Molecular Sciences   Vol. 23 ( 6 ) page: 3137   2022.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    Signal peptides (SPs) not only mediate targeting to the endoplasmic reticulum (ER) but also play important roles as biomarkers and substances with physiological activity in extracellular fluids including blood. SPs are thought to be degraded intracellularly, making it unclear how they are transported from the ER to the extracellular fluid. In a recent study, we showed that a C-terminal fragment of the SP of a type I membrane protein, amyloid precursor protein (APP), was secreted into the extracellular fluid via exosomes using transformed HEK293 cells expressing APP SP flanking a reporter protein. In the present study, we demonstrate that a N-terminal fragment of the SP from a type II membrane protein, human placental secreted alkaline phosphatase (SEAP), is contained in exosomes and secreted into the extracellular fluid using HEK-Blue hTLR3 cells, which express both a human toll-like receptor 3 gene and an inducible SEAP reporter gene. When HEK-Blue hTLR3 cells were stimulated with a TLR3 ligand, a N-terminal fragment of SEAP SP in exosomes was increased in parallel with SEAP secretion in a concentration-dependent manner. These results indicated that SP fragments are exosomal components. In addition, migrating SP fragments were determined by characteristics of the signal–anchor sequence of membrane proteins. Furthermore, we found that SP fragments could bind to calmodulin (CALM), which is a cytosolic protein and also a component of exosomes, suggesting its involvement in the transportation of SP fragments from the endoplasmic reticulum to exosomes.

    DOI: 10.3390/ijms23063137

    Other Link: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8950404/

  3. Secretion of signal peptides via extracellular vesicles Reviewed International journal

    Ono K, Niwa M, Suzuki H, Kobayashi NB, Yoshida T, Sawada M.

    Biochem Biophys Res Commun .   Vol. 560   page: 21 - 26   2021.5

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Elsevier  

    Signal peptides (SPs) consist of short peptide sequences present at the N-terminal of newly synthesizing proteins and act as a zip code for the translocation of the proteins to the endoplasmic reticulum (ER). It was thought that the SPs are intracellularly degraded after translocation to the ER; however, recent studies showed cleaved SPs have diverse roles for controlling cell functions in auto- and/or intercellular manners. In addition, it still remains obscure how SP fragments translocate away from the site where they are produced. Extracellular vesicles (EV) are important for intercellular communication and can transport functional molecules to specific cells. In this study, we show that SPs are involved in EV from T-REx AspALP cells that were transfected with a human APP SP-inducible expression vector. There was no difference in the average particle size or particle concentration of EV collected from T-REx AspALP cells and T-REx Mock cells. When the SP content in the EV was examined by mass spectrometry, the C-terminal fragment of APP SP was identified in the exosomes (SEV) of T-REx AspALP cells. In our preparation of SEV fractions, no ER-specific proteins were detected; therefore, SPs may be included in SEV but not in the debris of degraded ER. This is the first indication that SPs are secreted from cells via EV.

    DOI: 10.1016/j.bbrc.2021.04.073.

  4. Peripheral benzodiazepine receptor/18 kDa translocator protein positron emission tomography imaging in a rat model of acute brain injury Reviewed International coauthorship

    Nomura M, Toyama H, Suzuki H, Yamada T, Hatano K, Alan A Wilson, Ito K, Sawada M

    Ann Null Med.   Vol. 35 ( 1 ) page: 8 - 16   2021.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1007/s12149-020-01530-2

  5. Distribution of Signal Peptides in Microvesicles from Activated Macrophage Cells Invited Reviewed

    International Journal of Molecular Science   Vol. 24 ( 15 )   2023.7

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    Authorship:Lead author, Corresponding author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/ijms241512131

    Web of Science

    PubMed

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Books 7

  1. PET/CT for Inflammatory Diseases: Basic Sciences, Typical Cases, and Review Reviewed

    Hiromi Suzuki, Makoto Sawada( Role: Contributor ,  Role of Microglia in Neuroinflammation)

    Springer  2020.2  ( ISBN:978-981-15-0810-3

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    Total pages:234   Language:English Book type:Textbook, survey, introduction

    At present, common diseases of CNS were often chronic and progressive which could affect the patient's quality of life seriously. But the pathogenesis for most of them had not been fully understood and was no effective pre- vention or treatment. Certain pathological injury could cause cellular inflammatory response. Microglia, the first activated effector cells, played an important role in the immune defense process. Microglia had a two-way effect. On the one hand, activated microglia phagocytosed damaged cell debris and removed antigenic substances, and could release cyto- toxic factors which would aggravate the injury of neuron. On the other hand, activated microglia may accumulate around damaged neurons and might induce neurotrophin-dependent protective activity. Therefore, to study the mechanism of microglia in the diseases of CNS and limit their effects on neuronal injury may help to retard the procession of some chronic disease and enhance the therapeutic effect of acute CNS diseases. Microglia are expected to become a new tar- get for the treatment of neurodegenerative diseases and cen- tral nervous system diseases.

  2. アルツハイマー病 発症メカニズムと新規診断法・創薬・治療開発

    澤田 誠, 鈴木弘美( Role: Joint author)

    株式会社エヌ・ティ・エス  2018.8  ( ISBN:978-4-86043-578-3

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    Total pages:460   Language:Japanese Book type:Textbook, survey, introduction

  3. 脳卒中病態学のススメ・脳内マクロファージとミクログリア

    澤田 誠, 鈴木弘美( Role: Joint author)

    南山堂  2018.2  ( ISBN:978--4-525-24851-2

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    Language:Japanese

  4. Etiology and Pathophysiology of Parkinson's Disease: Role of microglia in inflammatory process, in Parkinson's disease

    Sawada H, Suzuki H, Ono K, Imamaura K, Nagatsu T, Sawada M( Role: Joint author)

    InTech  2011.10  ( ISBN:978-953-307-462-7

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    Language:English

  5. 遺伝子医学MOOK: 小動物PETによるラットパーキンソン病モデルの神経傷害性と治療効果判定

    外山 宏、籏野健太郎、鈴木弘美( Role: Joint author)

    メディカルドゥ  2010.10 

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    Language:Japanese

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MISC 18

  1. LC-MS/MS imaging with thermal film-based laser microdissection Reviewed

    Michiko Oya, Hiromi Suzuki, Andrea Roxanne, J. Anas, Koichi Oishi, Kenji Ono, Shun Yamaguchi, Megumi Eguchi, Makoto Sawada

    Anal Bioanal Chem   Vol. 410 ( 2 ) page: 491 - 499   2018.1

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    Authorship:Corresponding author   Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.1007/s00216-017-0739-2.

  2. Visualization of iNOS gene expression from activated cells in magnetic resonance imaging

    Kenji Ono, Kaori Tabata, Hiromi Suzuki, Makoto Sawada

    NEUROSCIENCE RESEARCH   Vol. 71   page: E96 - E96   2011

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    Language:English   Publishing type:Research paper, summary (international conference)  

    DOI: doi.org/10.1016/j.neures.2011.07.413

  3. Inducible nitric oxide synthase during the late phase of sepsis is associated with hypothermia and immune cell migration Reviewed

    Laboratory Investigation   Vol. 98 ( 5 ) page: 629 - 639   2018.5

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    Language:English   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

    DOI: 10.1038/s41374-018-0021-z

  4. Optogenetic control of cell differentiation in channelrhodopsin-2-expressing OS3, a bipotential glial progenitor cell line Reviewed International journal

    Kenji Ono, Hiromi Suzuki, Ryusei Yamamoto, Hideki Sahashi, Yuhei Takido, Makoto Sawada

    NEUROCHEMISTRY INTERNATIONAL   Vol. 104   page: 49 - 63   2017.3

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    Language:English   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)   Publisher:PERGAMON-ELSEVIER SCIENCE LTD  

    Alterations in the intracellular ion environment have been identified as one of the signals playing a critical role in the control of cellular proliferation and differentiation; however, the mechanisms responsible for signal transduction remain unclear. Recent studies have reported that channelrhodopsin-2 (ChR2) is a rapidly gated blue light (BL)-sensitive cation channel suitable for the non-invasive control of ion influx. We herein examined the expression of differentiation-associated markers by photo-activation and its signal transduction in ChR2-expressing OS3 (OS3ChR2) cells, which are clonal bipotential glial progenitor cells. Increases were observed in intracellular Na+ and Ca2+ concentrations in OS3ChR2 cells with BL exposure. Alterations in the intracellular ion environment, particularly in Ca2+, led to increases in the expression of oligodendrocyte markers including galactocerebrosides (GalC) and decreases in that of astrocyte markers such as glial fibrillary acidic protein (GFAP). These alterations also triggered activation of the ERK1/2 signaling pathway, which is involved in cell survival, and PI3K/Akt/mTOR signaling pathway, which is involved in oligodendrocyte differentiation, characterized by GalC expression. Moreover, when photo-activated OS3ChR2 cells were injected into mice with lysophosphatidyl choline (LPC)-induced demyelination, deficits in motor function were reduced. Our results demonstrated that signal transduction by ChR2-expressing glial progenitor cells may be controlled through alterations induced in the intracellular ion environment by photo-activation and results in oligodendrOcyte differentiation from glial progenitor cells. Our results also suggest that ChR2-expressing glial progenitor cells have potential as a useful tool for therapeutic approaches to brain and spinal cord disorders associated with oligodendrocyte dysfunctions. (C) 2017 Elsevier Ltd. All rights reserved.

    DOI: 10.1016/j.neuint.2016.12.022

  5. ミクログリアによるBBB非崩壊型の脳へのターゲティングDDS Invited

    澤田 誠, 鈴木弘美, 小野健治

    薬剤学   Vol. 71 ( 5 ) page: 259-267   2011

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    Language:Japanese   Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

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Presentations 86

  1. Expansion-LCM: Subcellular dissection reveals mitochondrial damage

    2024.11.26 

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    Event date: 2024.11

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  2. Inhibition of microglial activation and protection to dopaminergic neurons by a novel COX-2 inhibitor

    2023.12.8 

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    Event date: 2023.12

    Language:Japanese  

  3. Detection of activated microglia by mass spectrometry imaging

    Hiromi Suzuki, Kenji Ono, Makoto Sawada

    2022.11.30 

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    Event date: 2022.11 - 2022.12

    Language:Japanese   Presentation type:Poster presentation  

    Country:Japan  

  4. エクソソームを介したシグナルペプチドの細胞外放出

    小野健治、丹羽幹夫、鈴木弘美、小林ベイリー菜穂子、吉田徹彦、澤田 誠

    第95回日本生化学会大会   2022.11.9  名古屋大学 門松健治

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    Event date: 2022.11

    Language:Japanese  

    Venue:名古屋   Country:Japan  

  5. LC-MS/MS-SRMを用いた、マウス脳組織中のTSPOリガンドPK11195とFEPPAの同時定量

    4. ANAS Jocsing, 鈴木弘美, 小野健治, 外山 宏, 野村昌彦, 籏野健太郎,原田健一, 澤田 誠

    日本薬学会142年会  2022.3.26  名城大学 森 裕二

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    Event date: 2022.3

    Language:English   Presentation type:Poster presentation  

    Venue:Web開催   Country:Japan  

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Research Project for Joint Research, Competitive Funding, etc. 10

  1. 神経変性疾患の創薬標的たるマイクログリア特異的発現分子のPETイメージングの開発

    Grant number:18H02776  2018.4 - 2021.3

    国立研究開発法人 国立長寿医療研究センター  基盤研究 (B) 

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\150

  2. シグナルペプチド:細胞外微粒子機能の新規マーカー

    2017.10 - 2023.3

    CREST 

    鈴木弘美

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid)  Grant type:Competitive

    Grant amount:\191975680 ( Direct Cost: \185697499 、 Indirect Cost:\6298719 )

  3. Role of diabetes and inflammation on microglial cell biology and transformation International coauthorship

    2020.10 - 2021.10

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    Authorship:Coinvestigator(s)  Grant type:Collaborative (industry/university)

    Grant amount:\24670482 ( Direct Cost: \24670482 )

  4. 新規な脳腫瘍診断マーカー遺伝子の探索

    2023.4

    医療法人 今井病院 今井文博  共同研究費 

    鈴木弘美

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    Authorship:Principal investigator  Grant type:Collaborative (industry/university)

    Grant amount:\460000 ( Direct Cost: \460000 )

  5. エクソソームの大量調製条件の検討

    2023.4 - 2027.3

    共同研究費 

    鈴木弘美

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    Authorship:Principal investigator  Grant type:Collaborative (industry/university)

    Grant amount:\1300000 ( Direct Cost: \1200000 、 Indirect Cost:\100000 )

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KAKENHI (Grants-in-Aid for Scientific Research) 3

  1. 神経変性疾患の創薬標的たるマイクログリア特異的発現分子のPETイメージングの開発

    Grant number:18H02776  2018.4 - 2021.3

    国立研究開発法人 国立長寿医療研究センター   基盤研究 (B) 

    木村泰之

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    Authorship:Coinvestigator(s)  Grant type:Competitive

    Grant amount:\500000 ( Direct Cost: \500000 、 Indirect Cost:\30000 )

  2. Imaging of neuroimmunoreactlve response-Multilateral validation uslng newly developed PBR ligands for positron emlssion computed tomography

    Grant number:21390350  2009 - 2011

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

    TOYAMA Hiroshi, HATANO Kentaro, SAWADA Makoto, KUDO Gen, YAMADA Takashi, NOMURA Masahiko, OTA Seiichiro, SUZUKI Hiromi, ICHISE Masanori, TRAPANI Giuseppe, WILSON Alan A.

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    Ncuro-reccpt on(peripheral benzodiazepine receptor : PBR/translocat or prat ein : TSPO) positron emission CT(PET) imaging and histological findings were compared in rat neuro-inflammat ion model under lipopolysaccharide(LPS) administration to ampli ry the inF1anmat. ion i n raperi onea 1 ly. Increased bindings of neuro-receptor(PBR/TSPO) PET imaging were shown under cytotoxic damage, act ivated gl i a cell(mi croglia) and increased expression of inflammatory cytokines. These results suggest. that. increased PBR/TSPO binding by PET appears a promising for early diagnosis of neuro-degenerative disorders.

  3. Basic research of molecular imaging for early diagnosis of Alzheimer's disease

    Grant number:18591369  2006 - 2008

    Japan Society for the Promotion of Science  Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    TOYAMA Hiroshi, HATANO Kentaro, SAWADA Makoto, ITO Kengo, KATO Takashi, KATADA Kazuhiro, KUDO Gen, ITO Fumitaka, OHASHI Masao, SUZUKI Hiromi, NAKANE Masato, ICHISE Masanori, GIUSEPPE Trapani, ALAN A Wilson

Industrial property rights 7

  1. 質量分析方法

    東海国立大学機構,JSR,島津製作所

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    Applicant:澤田 誠, 小野健治, 鈴木弘美, 王 勇, 緒方是嗣, 村田 匡

    Application no:PCT/JP2021/044239  Date applied:2021.12

    Announcement no:WO2022/131000  Date announced:2022.6

    Country of applicant:Domestic , Foreign country   Country of acquisition:Domestic , Foreign country

  2. レーザマイクロダイセクション装置

    澤田 誠, 鈴木弘美, 小野健治, 洪 暎淳

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    Applicant:東海国立大学機構, 島津製作所

    Application no:PCT/JP2020/011793  Date applied:2020.3

    Announcement no:WO 2021/186577  Date announced:2021.9

    Country of applicant:Domestic , Foreign country   Country of acquisition:Domestic , Foreign country

  3. 多価結合手を有し代謝安定性が向上した脳移行性ポリペプチド

    中條 智洋, 原 啓高, 山本 一匡, 鈴木 弘美, 澤田 誠, 須原 哲也, 樋口 真人, 原田平 輝志, 季 斌

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    Applicant:プロテウス・サイエンス株式会社、国立放射線科学研究所

    Application no:特願2009-502538  Date applied:2008.2

    Publication no:WO 2008/108242 A1  Date published:2008.9

    Patent/Registration no:特許第5250849号  Date registered:2013.4 

    Country of applicant:Domestic , Foreign country   Country of acquisition:Domestic , Foreign country

    J-GLOBAL

  4. 金属コロイド粒子を含有する脳神経細胞内移行用キャリア

    澤田 誠, 鈴木 弘美

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    Applicant:株式会社ティッシュターゲティングジャパン

    Application no:JP2005001761  Date applied:2005.2

    Announcement no:WO 2006/013650 A1  Date announced:2006.2

    Publication no:WO2006-013650  Date published:2006.2

    Country of applicant:Domestic , Foreign country   Country of acquisition:Domestic , Foreign country

    J-GLOBAL

  5. PESI-MSでのイオン化の効率を上げる手法

    澤田 誠, 小野健治, 鈴木弘美, 王 勇, 緒方是嗣, 村田 匡

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    Applicant:東海国立大学機構, JSR, 島津製作所

    Application no:WUS0001418 

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Teaching Experience (On-campus) 13

  1. 環境学入門

    2024

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    神経系を構成するグリア細胞の役割という内容で講義を行う
    グリア細胞であるミクログリアを中心に、病態やその機能についてわかりやすく説明・授業を行う

  2. ベーシックトレーニング

    2024

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    質量分析イメージングの原理とその応用方法について講義を行う。マウス脳より凍結切片を作製し、質量分析イメージングを行う前処理・イメージ取得について実習し、取得したデータの解析についても学ぶ。

  3. ベーシックトレーニング

    2023

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    顕微鏡下で組織サンプルの画像取得後、画像データを基に病態由来の細胞を同定・解析し、座標再現機能を装着したマイクロディセクション顕微鏡を用いて半自動操作により採取・分離する技術について実践します。さらに、分取細胞の質量分析を行い、分析結果を元の画像に重ねてLC-MS/MALDI 質量分析イメージングにチャレンジします。

  4. 基礎医学セミナー

    2022

  5. ベーシックトレーニング

    2022

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Teaching Experience (Off-campus) 4

  1. 大学院講義

    2019.4 - 2020.3 Fujita Health University)

  2. 2018.4 - 2019.3 Fujita Health University)

  3. ベーシックトレーニング

    Nagoya University)

  4. ベーシックトレーニング

    Nagoya University)

 

Social Contribution 1

  1. 公益社団法人 生体制御学会 運営

    Role(s):Editer

    2024.8