Updated on 2022/04/21

写真a

 
HAYASHI, Yoshitaka
 
Organization
Research Institute of Environmental Medicine Division of Stress Adaptation and Protection Professor
Graduate School
Graduate School of Medicine
Title
Professor
Contact information
メールアドレス

Degree 1

  1. 博士(医学) ( 1995.8   名古屋大学 ) 

Research Interests 1

  1. endocrinology, pancreatic islet, thyroid, cell differentiation, transcriptional regulation, stem cell, genetics, diabetes mellitus, developmental biology, nuclear receptor, extracellular matrix, transcriptome/metabolome analyses

Research Areas 5

  1. Life Science / Physiology  / Endocrinology

  2. Life Science / Physiology  / Metabolism Studies

  3. Life Science / General internal medicine  / Diabetology

  4. Life Science / Molecular biology  / Pathological Medical Chemistry

  5. Life Science / Molecular biology  / Genetics

Current Research Project and SDGs 4

  1. Endocrinology and Molecular Biology using glucagon-gene modified model.

  2. pathophysiology of malignant tumors derived from endocrine tissues

  3. mechanism of differentiation endoderm cells/tissues

  4. pathophysiology of inherited endocrine disease

Research History 7

  1. Research Institute of Environmental Medicine, Nagoya University   Department of Endocrinology, Division of Stress Adaptation and Protection   Professor

    2019.4

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    Country:Japan

  2. Research Institute of Environmental Medicine, Nagoya University   Division of Stress Adaptation and Protection   Associate professor

    2007.4 - 2019.3

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    Country:Japan

  3. Research Institute of Environmental Medicine, Nagoya University   Division of Molecular and Cellular Adaptation   Assistant Professor

    2005.10 - 2007.3

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    Country:Japan

  4. JST   ,Sekiguchi Biomatrix Signaling Project, ERATO   Group Leader (Researcher)

    2000.10 - 2005.9

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    Country:Japan

  5. Nagoya University   Research Institute of Environmental Medicine   JSPS Research Fellow

    1995.4 - 1998.3

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    Country:Japan

  6. University of Chicago   Department of Medicine   Research Associate (Assistant Professor)

    1994.11 - 1995.3

  7. University of Chicago   Department of Medicine   Researcher

    1991.11 - 1994.10

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Education 2

  1. Nagoya University   Graduate School, Division of Medicine

    1989.4 - 1995.8

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    Country: Japan

  2. Nagoya University   Faculty of Medicine

    1981.4 - 1987.3

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    Country: Japan

Professional Memberships 8

  1. 日本内分泌学会   評議員

  2. 日本糖尿病学会   学術評議員

  3. Japan Thyroid Association

  4. 日本内科学会

  5. The Endocrine Society

  6. 日本病態栄養学会

  7. 日本分子生物学会

  8. 日本生理学会

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Awards 2

  1. 日本内分泌学会 研究奨励賞

    2000   日本内分泌学会  

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    Country:Japan

  2. Shichijo Prize

    2000   Japan Thyroid Association  

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    Country:Japan

 

Papers 106

  1. Glutaminostatin: Another facet of glucagon as a regulator of plasma amino acid concentrations Reviewed

    Hayashi Yoshitaka

    JOURNAL OF DIABETES INVESTIGATION   Vol. 10 ( 6 ) page: 1391 - 1393   2019.11

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jdi.13110

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  2. Regulation of amino acid metabolism and α-cell proliferation by glucagon Reviewed

    Yoshitaka Hayashi, Yusuke Seino

    Journal of Diabetes Investigation   Vol. 9 ( 3 ) page: 464 - 472   2018.1

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    Language:English   Publishing type:Research paper (scientific journal)   Publisher:Blackwell Publishing  

    Both glucagon and glucagon-like peptide-1 (GLP-1) are produced from proglucagon through proteolytic cleavage. Blocking glucagon action increases the circulating levels of glucagon and GLP-1, reduces the blood glucose level, and induces the proliferation of islet α-cells. Glucagon blockade also suppresses hepatic amino acid catabolism and increases the serum amino acid level. In animal models defective in both glucagon and GLP-1, the blood glucose level is not reduced, indicating that GLP-1 is required for glucagon blockade to reduce the blood glucose level. In contrast, hyperplasia of α-cells and hyperaminoacidemia are observed in such animal models, indicating that GLP-1 is not required for the regulation of α-cell proliferation or amino acid metabolism. These findings suggest that the regulation of amino acid metabolism is a more important specific physiological role of glucagon than the regulation of glucose metabolism. Although the effects of glucagon deficiency on glucose metabolism are compensated by the suppression of insulin secretion, the effects on amino acid metabolism are not. Recently, data showing a feedback regulatory mechanism between the liver and islet α-cells, which is mediated by glucagon and amino acids, are accumulating. However, a number of questions on the mechanism of this regulation remain to be addressed. The profile of glucagon as a regulator of amino acid metabolism must be carefully considered for glucagon blockade to be applied therapeutically in the treatment of patients with diabetes.

    DOI: 10.1111/jdi.12797

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  3. *Remodeling of Hepatic Metabolism and Hyperaminoacidemia in Mice Deficient in Proglucagon-derived Peptides. Reviewed

    C. Watanabe, Y. Seino, H. Miyahira, M. Yamamoto, A. Fukami, N. Ozaki, Y. Takagishi, J. Sato, T. Fukuwatari, K. Shibata, Y. Oiso, Y. Murata, Y. Hayashi

    Diabetes   Vol. 61 ( 1 ) page: 74-84   2012.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.2337/db11-0739

  4. *Metabolic impact of glucagon deficiency. Invited Reviewed

    Yoshitaka Hayashi

    Diabetes, Obesity and Metabolism.   Vol. 13 ( suppl 1 ) page: 151-157   2011.10

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/j.1463-1326.2011.01456.x

  5. *Mice Deficient for Glucagon Gene-Derived Peptides Display Normoglycemia, and Hyperplasia of Islet a-Cells but not of Intestinal L-cells. Reviewed

    Y. Hayashi, M. Yamamoto, H. Mizoguchi, C. Watanabe, R. Ito, S. Yamamoto, X.Y. Sun, Y. Murata

    Mol Endocrinol   Vol. 23 ( 12 ) page: 1990-1999   2009.12

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  6. Acute Cholesterol-Lowering Effect of Exendin-4 in Ldlr<sup>-/-</sup> and C57BL/6J Mice. Reviewed

    Hori M, Hasegawa Y, Hayashi Y, Nakagami T, Harada-Shiba M

    Journal of atherosclerosis and thrombosis     2022.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.5551/jat.60921

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  7. High Protein Diet Feeding Aggravates Hyperaminoacidemia in Mice Deficient in Proglucagon-Derived Peptides Reviewed

    Ueno Shinji, Seino Yusuke, Hidaka Shihomi, Maekawa Ryuya, Takano Yuko, Yamamoto Michiyo, Hori Mika, Yokota Kana, Masuda Atsushi, Himeno Tatsuhito, Tsunekawa Shin, Kamiya Hideki, Nakamura Jiro, Kuwata Hitoshi, Fujisawa Haruki, Shibata Megumi, Takayanagi Takeshi, Sugimura Yoshihisa, Yabe Daisuke, Hayashi Yoshitaka, Suzuki Atsushi

    NUTRIENTS   Vol. 14 ( 5 )   2022.3

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/nu14050975

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  8. Carbonic anhydrase 8 (CAR8) negatively regulates GLP-1 secretion from enteroendocrine cells in response to long-chain fatty acids Reviewed

    Fujiwara Yuta, Yamane Shunsuke, Harada Norio, Ikeguchi-Ogura Eri, Usui Ryota, Nakamura Toshihiro, Iwasaki Kanako, Suzuki Kazuyo, Yabe Daisuke, Hayashi Yoshitaka, Inagaki Nobuya

    AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY   Vol. 320 ( 4 ) page: G617 - G626   2021.4

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1152/ajpgi.00312.2020

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  9. Carbohydrate-induced weight gain models for diabetes research: contribution of incretins and parasympathetic signal. Reviewed

    Seino Y, Murase M, Hayashi Y, Suzuki A

    Journal of diabetes investigation   Vol. 12 ( 1 ) page: 3 - 5   2021.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jdi.13342

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  10. Deficiency of glucagon gene-derived peptides induces peripheral polyneuropathy in mice Reviewed

    Motegi Mikio, Himeno Tatsuhito, Nakai-Shimoda Hiromi, Inoue Rieko, Ozeki Norio, Hayashi Yusuke, Sasajima Sachiko, Mohiuddin Mohammad Sarif, Asano-Hayami Emi, Kato Makoto, Asano Saeko, Miura-Yura Emiri, Morishita Yoshiaki, Kondo Masaki, Tsunekawa Shin, Kato Yoshiro, Kato Koichi, Naruse Keiko, Seino Yusuke, Hayashi Yoshitaka, Nakamura Jiro, Kamiya Hideki

    BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS   Vol. 532 ( 1 ) page: 47 - 53   2020.10

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1016/j.bbrc.2020.08.007

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  11. Short-Term High-Starch, Low-Protein Diet Induces Reversible Increase in -cell Mass Independent of Body Weight Gain in Mice Reviewed

    Masuda Atsushi, Seino Yusuke, Murase Masatoshi, Hidaka Shihomi, Shibata Megumi, Takayanagi Takeshi, Sugimura Yoshihisa, Hayashi Yoshitaka, Suzuki Atsushi

    NUTRIENTS   Vol. 11 ( 5 )   2019.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.3390/nu11051045

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  12. Functional adenosine triphosphate-sensitive potassium channel is required in high-carbohydrate diet-induced increase in β-cell mass. Reviewed

    Masatoshi Murase, Yusuke Seino, Ryuya Maekawa, Atsushi Iida, Kaori Hosokawa, Tomohide Hayami, Shin Tsunekawa, Yoji Hamada, Norihide Yokoi, Susumu Seino, Yoshitaka Hayashi, Hiroshi Arima

    Journal of diabetes investigation   Vol. 10 ( 2 ) page: 238 - 250   2019.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    AIMS/INTRODUCTION: A high-carbohydrate diet is known to increase insulin secretion and induce obesity. However, whether or not a high-carbohydrate diet affects β-cell mass (BCM) has been little investigated. MATERIALS AND METHODS: Both wild-type (WT) mice and adenosine triphosphate-sensitive potassium channel-deficient (Kir6.2KO) mice were fed normal chow or high-starch (ST) diets for 22 weeks. BCM and the numbers of islets were analyzed by immunohistochemistry, and gene expression levels in islets were investigated by quantitative real-time reverse transcription polymerase chain reaction. MIN6-K8 β-cells were stimulated in solution containing various concentrations of glucose combined with nifedipine and glimepiride, and gene expression was analyzed. RESULTS: Both WT and Kir6.2KO mice fed ST showed hyperinsulinemia and body weight gain. BCM, the number of islets and the expression levels of cyclinD2 messenger ribonucleic acid were increased in WT mice fed ST compared with those in WT mice fed normal chow. In contrast, no significant difference in BCM, the number of islets or the expression levels of cyclinD2 messenger ribonucleic acid were observed between Kir6.2KO mice fed normal chow and those fed ST. Incubation of MIN6-K8 β-cells in high-glucose media or with glimepiride increased cyclinD2 expression, whereas nifedipine attenuated a high-glucose-induced increase in cyclinD2 expression. CONCLUSIONS: These results show that a high-starch diet increases BCM in an adenosine triphosphate-sensitive potassium channel-dependent manner, which is mediated through upregulation of cyclinD2 expression.

    DOI: 10.1111/jdi.12907

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  13. Distribution and hormonal characterization of primary murine L cells throughout the gastrointestinal tract Reviewed

    Suzuki Kazuyo, Iwasaki Kanako, Murata Yuki, Harada Norio, Yamane Shunsuke, Hamasaki Akihiro, Shibue Kimitaka, Joo Erina, Sankoda Akiko, Fujiwara Yuta, Hayashi Yoshitaka, Inagaki Nobuya

    JOURNAL OF DIABETES INVESTIGATION   Vol. 9 ( 1 ) page: 25 - 32   2018.1

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1111/jdi.12681

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  14. Glucagon regulates lipolysis and fatty acid oxidation through inositol triphosphate receptor 1 in the liver Reviewed

    Hayashi Yoshitaka

    JOURNAL OF DIABETES INVESTIGATION   Vol. 12 ( 1 ) page: 32 - 34   2021.1

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    DOI: 10.1111/jdi.13315

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  15. Sphingosine kinase 1–interacting protein is a dual regulator of insulin and incretin secretion Reviewed

    Yanyan Liu, Shin Ichi Harashima, Yu Wang, Kazuyo Suzuki, Shinsuke Tokumoto, Ryota Usui, Hisato Tatsuoka, Daisuke Tanaka, Daisuke Yabe, Norio Harada, Yoshitaka Hayashi, Nobuya Inagaki

    FASEB Journal   Vol. 33 ( 5 ) page: 6239 - 6253   2019.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    © FASEB Our previous study demonstrated that sphingosine kinase 1–interacting protein (SKIP, or Sphkap) is expressed in pancreatic β-cells, and depletion of SKIP enhances glucose-stimulated insulin secretion. We find here that SKIP is also expressed in intestinal K- and L-cells and that secretion of gastric inhibitory polypeptide (GIP) and glucagon-like peptide-1 (GLP-1) as well as insulin are significantly increased, and blood glucose levels are decreased in SKIP-deficient (SKIP−/−) mice compared with those in wild-type mice. Plasma triglyceride (Tg), LDL cholesterol, and mRNA levels of proinflammatory cytokines in adipose tissues, livers, and intestines were found to be significantly decreased in SKIP−/− mice. The phenotypic characteristics of SKIP−/− mice, including adiposity and attenuation of basal inflammation, were abolished by genetic depletion of GIP. The improvement of glucose tolerance and lipid profiles in SKIP−/− mice were cancelled by GLP-1 receptor antagonist exendin-(9–39) treatment. In summary, depletion of SKIP ameliorates glucose tolerance by enhancing secretion of insulin and incretins, improves lipid metabolism, and reduces basal inflammation levels. Thus, inhibition of SKIP action may emerge as a new option for treatment of type 2 diabetes mellitus with metabolic dysfunction.—Liu, Y., Harashima, S., Wang, Y., Suzuki, K., Tokumoto, S., Usui, R., Tatsuoka, H., Tanaka, D., Yabe, D., Harada, N., Hayashi, Y., Inagaki, N. Sphingosine kinase 1–interacting protein is a dual regulator of insulin and incretin secretion. FASEB J. 33, 6239–6253 (2019). www.fasebj.org.

    DOI: 10.1096/fj.201801783RR

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  16. Two Mutations in Thyroid Hormone Receptor Beta Gene (P453A and C36Y) in a Family with Resistance to Thyroid Hormone with Comorbid Myotonic Dystrophy. Reviewed

    Takeda K, Nemoto KI, Hayashi Y, Yamamoto M, Sakuta R, Kimura T, Noto H

    Thyroid : official journal of the American Thyroid Association   Vol. 29 ( 4 ) page: 607 - 608   2019.4

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    DOI: 10.1089/thy.2018.0307

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  17. Glucose-dependent insulinotropic polypeptide is required for moderate high-fat diet-but not high-carbohydrate diet-induced weight gain Reviewed

    Ryuya Maekawa, Hidetada Ogata, Masatoshi Murase, Norio Harada, Kazuyo Suzuki, Erina Joo, Akiko Sankoda, Atsushi Iida, Takako Izumoto, Shin Tsunekawa, Yoji Hamada, Yutaka Oiso, Nobuya Inagaki, Hiroshi Arima, Yoshitaka Hayashi, Yusuke Seino

    American Journal of Physiology - Endocrinology and Metabolism   Vol. 314 ( 6 ) page: E572 - E583   2018.6

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    © 2018 American Physiological Society. All rights reserved. Both high-fat (HFD) and high-carbohydrate (ST) diets are known to induce weight gain. Glucose-dependent insulinotropic polypeptide (GIP) is secreted mainly from intestinal K cells upon stimuli by nutrients such as fat and glucose, and it potentiates glucose-induced insulin secretion. GIP is well known to contribute to HFD-induced obesity. In this study, we analyzed the effect of ST feeding on GIP secretion and metabolic parameters to explore the role of GIP in ST-induced weight gain. Both wild-type (WT) and GIP receptor deficient (GiprKO) mice were fed normal chow (NC), ST, or moderate (m)HFD for 22 wk. Body weight was measured, and then glucose tolerance tests were performed. Insulin secretion from isolated islets also was analyzed. WT mice fed ST or mHFD displayed weight gain concomitant with increased plasma GIP levels compared with WT mice fed NC. WT mice fed mHFD showed improved glucose tolerance due to enhanced insulin secretion during oral glucose tolerance tests compared with WT mice fed NC or ST. GiprKO mice fed mHFD did not display weight gain. On the other hand, GiprKO mice fed ST showed weight gain and did not display obvious glucose intolerance. Glucose-induced insulin secretion was enhanced during intraperitoneal glucose tolerance tests and from isolated islets in both WT and GiprKO mice fed ST compared with those fed NC. In conclusion, enhanced GIP secretion induced by mHFD-feeding contributes to increased insulin secretion and body weight gain, whereas GIP is marginally involved in weight gain induced by ST-feeding.

    DOI: 10.1152/ajpendo.00352.2017

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  18. The Mutant Thyroid Hormone Receptor Beta R320P Causes Syndrome of Resistance to Thyroid Hormone Reviewed

    Kimura Tetsuya, Hayashi Yoshitaka, Tsukamoto Yuka, Okamoto Yasuyuki

    CASE REPORTS IN ENDOCRINOLOGY   Vol. 2018   page: 4081769   2018

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    DOI: 10.1155/2018/4081769

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  19. Chronic high-sucrose diet increases fibroblast growth factor 21 production and energy expenditure in mice. Reviewed

    Ryuya Maekawa, Yusuke Seino, Hidetada Ogata, Masatoshi Murase, Atsushi Iida, Kaori Hosokawa, Erina Joo, Norio Harada, Shin Tsunekawa, Yoji Hamada, Yutaka Oiso, Nobuya Inagaki, Yoshitaka Hayashi, Hiroshi Arima

    J Nutr Biochem   Vol. 49   page: 71-79   2017.11

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    DOI: doi: 10.1016/j.jnutbio.2017.07.010.

  20. Chronic high-sucrose diet increases fibroblast growth factor 21 production and energy expenditure in mice Reviewed

    Maekawa Ryuya, Seino Yusuke, Ogata Hidetada, Murase Masatoshi, Iida Atsushi, Hosokawa Kaori, Joo Erina, Harada Norio, Tsunekawa Shin, Hamada Yoji, Oiso Yutaka, Inagaki Nobuya, Hayashi Yoshitaka, Arima Hiroshi

    JOURNAL OF NUTRITIONAL BIOCHEMISTRY   Vol. 49   page: 71 - 79   2017.11

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    DOI: 10.1016/j.jnutbio.2017.07.010

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  21. Endogenous GIP ameliorates impairment of insulin secretion in proglucagon-deficient mice under moderate beta cell damage induced by streptozotocin. Reviewed

    Atsushi Iida, Yusuke Seino, Ayako Fukami, Ryuya Maekawa, Daisuke Yabe, Shinobu Shimizu, Keita Kinoshita, Yusuke Takagi, Takako Izumoto, Hidetada Ogata, Kota Ishikawa, Nobuaki Ozaki, Shin Tsunekawa, Yoji Hamada, Yutaka Oiso, Hiroshi Arima, Yoshitaka Hayashi,

    Diabetologia   Vol. 59 ( 7 ) page: 1533-1541   2016.4

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    DOI: doi: 10.1007/s00125-016-3935-2.

  22. Mice Deficient in Proglucagon-Derived Peptides Exhibit Glucose Intolerance on a High-Fat Diet but Are Resistant to Obesity.

    Takagi Y, Kinoshita K, Ozaki N, Seino Y, Murata Y, Oshida Y, Hayashi Y.

    PLoS One.   Vol. 10 ( 9 ) page: e0138322   2015.9

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    DOI: 10.1371/journal.pone.0138322.

  23. Chronic Hyponatremia Causes Neurologic and Psychologic Impairments.

    Fujisawa H, Sugimura Y, Takagi H, Mizoguchi H, Takeuchi H, Izumida H, Nakashima K, Ochiai H, Takeuchi S, Kiyota A, Fukumoto K, Iwama S, Takagishi Y, Hayashi Y, Arima H, Komatsu Y, Murata Y, Oiso Y.

    J Am Soc Nephrol.     page: Epub ahead of print   2015.9

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  24. *Pancreatic Neuroendocrine Tumors in Mice Deficient in Proglucagon-Derived Peptides.

    Takano Y, Kasai K, Takagishi Y, Kikumori T, Imai T, Murata Y, Hayashi Y.

    PLoS ONE   Vol. 10 ( 7 ) page: e0133812   2015.7

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    DOI: 10.1371/journal.pone.0133812.

  25. グルカゴンの真の特異的作用は何か?グルカゴン作用欠損モデルの表現型.

    林 良敬

    実験医学   Vol. 33 ( 6 ) page: 897-902   2015.4

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  26. Low temperature-induced circulating triiodothyronine accelerates seasonal testicular regression.

    K. Ikegami, Y. Atsumi, E. Yonenaga, H. Ono, I. Murayama, Y. Nakane, W. Ota, N. Arai, A. Tega, M. Iigo, V. M. Darras, K. Tsutsui, Y. Hayashi, S. Yoshida, Y. Yoshimura

    Endocrinology   Vol. 156 ( 2 ) page: 647-659   2015.2

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    DOI: 10.1210/en.2014-1741

  27. Fatty acid-binding protein 5 regulates diet-induced obesity via GIP secretion from enteroendocrine K cells in response to fat ingestion.

    Shibue K, Yamane S, Harada N, Hamasaki A, Suzuki K, Joo E, Iwasaki K, Nasteska D, Harada T, Hayashi Y, Adachi Y, Owada Y, Takayanagi R, Inagaki N.

    Am J Physiol Endocrinol Metab.   Vol. 308 ( 7 ) page: E583-E591   2015.1

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    DOI: 10.1152/ajpendo.00543.2014.

  28. Activation of the RhoB signaling pathway by thyroid hormone receptor ß in thyroid cancer cells.

    Ichijo S, Furuya F, Shimura H, Hayashi Y, Takahashi K, Ohta K, Kobayashi T, Kitamura K.

    PLoS One   Vol. 9 ( 12 ) page: e116252   2014.12

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    DOI: doi: 10.1371/journal.pone.0116252

  29. Glucagon is essential for adaptive thermongenesis in brown adipose tissue Reviewed

    K. Kinoshita, N. Ozaki, Y. Takagi, Y. Murata, Y. Oshida, Y. Hayashi

    Endocrinology   Vol. 155 ( 9 ) page: 3484-3492   2014.6

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    DOI: 10.1210/en.2014-1175.

  30. KATP channel as well as SGLT1 participates in GIP secretion in the diabetic state. Reviewed

    H. Ogata, Y. Seino, N. Harada, A. Iida, K. Suzuki, T. Izumoto, K. Ishikawa, E. Uenishi, N. Ozaki, Y. Hayashi, T. Miki, N. Inagaki, S. Tsunekawa, Y. Hamada, S. Seino, Y. Oiso

    Journal of ENdocrinology   Vol. 222 ( 2 ) page: 191-200   2014.6

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    DOI: 10.1111/jdi.12129.

  31. Sensory and motor physiological functions are impaired in gastric inhibitory polypeptide receptor-deficient mice.

    Okawa T, Kamiya H, Himeno T, Seino Y, Tsunekawa S, Hayashi Y, Harada N, Yamada Y, Inagaki N, Seino Y, Oiso Y, Nakamura J.

    J Diabetes Investig   Vol. 5 ( 1 ) page: 31-37   2013.10

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    DOI: 10.1111/jdi.12129

  32. Hyperthyroidism due to thyroid-stimulating hormone secretion after surgery for Cushing's syndrome: a novel cause of the syndrome of inappropriate secretion of thyroid-stimulating hormone. Reviewed

    D. Tamada, T. Onodera, T. Kitamura, Y. Yamamoto, Y. Hayashi, Y. Murata, M. Otsuki, I. Shimomura

    J Clin Endocrinol Metab   Vol. 98 ( 7 ) page: 2656-2662   2013.5

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    DOI: doi: 10.1210/jc.2013-2135

  33. Aristaless-related homeobox plays a key role in hyperplasia of the pancreas islet α-like cells in mice deficient in proglucagon-derived peptides. Reviewed

    S. Xu, Y. Hayashi, Y. Takagishi, M. Itoh, Y. Murata

    PLoS One   Vol. 8 ( 5 ) page: e64415   2013.5

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    DOI: doi: 10.1371/journal.pone.0064415

  34. Ectopic expression of GIP in pancreatic ß-cells maintains enhanced insulin secretion in mice with complete absence of proglucagon-derived peptides. Reviewed

    A. Fukami, Y. Seino, N. Ozaki, M. Yamamoto, C. Sugiyama, E. Sakamoto-Miura, T. Himeno, Y. Takagishi, S. Tsunekawa, S. Ali, D. J. Drucker, Y. Murata, Y. Seino, Y. Oiso, Y. Hayashi

    Diabetes     page: doi: 10.2337/db12-0294   2012.10

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    DOI: doi: 10.2337/db12-0294

  35. グルカゴンによる代謝制御 Invited

    林 良敬

    糖尿病と代謝   Vol. 40 ( 3 ) page: 1-8   2012.9

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  36. Fertility and pregnancy-associated ß-cell proliferation in mice deficient in proglucagon-derived peptides. Reviewed

    C. Sugiyama, M. Yamamoto, T. Kotani, F. Kikkawa, Y. Murata, Y. Hayashi

    Plos One     2012.8

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    DOI: 10.1371/journal.pone.0043745

  37. Isolated growth hormone deficiency in two siblings because of paternal mosaicism for a mutation in the GH1 gene. Reviewed

    M. Tsubahara, Y. Hayashi, S. Niijima, M. Yamamoto, T. Kamijo, Y. Murata, H. Haruna, A. Okumura, T. Shimizu

    Clin Endocrinol (Oxf.)   Vol. 76 ( 3 ) page: 420-424   2012.3

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    DOI: 10.1111/j.1365-2265.2011.04224.x.

  38. Aberrant promoter methylation in overexpression of CITED1 in papillary thyroid cancer. Reviewed

    M. Sassa, Y. Hayashi, R. Watanabe, T. Kikumori, T. Imai, J. Kurebayashi, T. Kiuchi, Y. Murata

    Thyroid   Vol. 21 ( 5 ) page: 511-517   2011.5

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  39. A novel Caspr mutation causes the shambling mouse phenotype by disrupting axo-glial interactions of myelinated nerves. Reviewed

    X.Y. Sun, Y. Takagishi, E. Okabe, Y. Chishima, Y. Kanou, S. Murase, K. Mizumura, M. Inaba, Y. Komatsu, Y. Hayashi, E. Peles, S. Oda, Y. Murata

    J Neuropathol Exp Neurol   Vol. 68 ( 11 ) page: 1207-1218   2009.11

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  40. Possible involvement of BRAFV600E in altered gene expression in papillary thyroid cancer. Invited Reviewed

    R. Watanabe, Y. Hayashi, M. Sassa, T. Kikumori, T. Imai, T. Kiuchih, Y. Murata

    Endocr J   Vol. 56   page: 407-414   2009.6

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  41. Prevention of neural tube defects by loss of function of inducible nitric oxide synthase in fetuses of a mouse model of streptozotocin-induced diabetes. Reviewed

    Y. Sugimura, T. Murase, K. Oyama, A. Uchida, N. Sato, S. Hayasaka, Y. Kano, Y. Takagishi, Y. Hayashi, Y. Oiso, Y. Murata

    Diabetologia   Vol. 56   page: 962-971   2009.5

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  42. Oculocutaneous Albinism in Suncus murinus: Establishment of a Strain and Identification of its Responsible Gene. Reviewed

    K. Tsuboi, Y. Hayashi, T. Jogahara, G. Ogura, Y. Murata,S. Oda

    Exp Anim   Vol. 58 ( 1 ) page: 31-40   2009.1

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  43. Laminin isoforms containing the gamma3 chain are unable to bind to integrins due to the absence of the glutamic acid residue conserved in the C-terminal regions of the gamma1 and gamma2 chains. Reviewed

    Ido H, Ito S, Taniguchi Y, Hayashi M, Sato-Nishiuchi R, Sanzen N, Hayashi Y, Futaki S, Sekiguchi K

    J Biol Chem   Vol. 283 ( 42 ) page: 28149-28157   2008.10

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  44. Identification of genes differentially expressed in mouse fetuses from streptozotocin-induced diabetic pregnancy by cDNA subtraction. Reviewed

    Sato N, Sugimura Y, Hayashi Y, Murase T, Kanou Y, Kikkawa F, Murata Y

    Endocr J   Vol. 55 ( 2 ) page: 317-323   2008.5

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  45. Insertion of an intracisternal A particle retrotransposon element in plasma membrane calcium ATPase 2 gene attenuates its expression and produces an ataxic phenotype in joggle mutant mice. Reviewed

    Sun XY, Chen ZY, Hayashi Y, Kanou Y, Takagishi Y, Oda S, Murata Y

    Gene   Vol. 411 ( 1-2 ) page: 94-102   2008.3

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  46. Long-term amiodarone treatment causes cardioselective hypothyroid-like alteration in gene expression profile. Reviewed

    R.Q. Shi, J. K. Lee, Y. Hayashi, Y. Takeuchi, F. Kambe, S. Futaki, H. Seo, Y. Murata, I. Kodama

    Eur J Parmacol   Vol. 578   page: 270-278   2008

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  47. Mapping of jog locus to the region between D6Mit104 and D6Mit336 on mouse chromosome 6. Reviewed

    Sun XY, Chen ZY, Kanou Y, Hayashi Y, Ohno T, Murata Y, Oda S

      Vol. 56 ( 5 ) page: 389-392   2007.10

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  48. A case with isolated growth hormone deficiency caused by compound heterozygous mutations in GH-1: a novel missense mutation in the initiation codon and a 7.6kb deletion Reviewed

    Hayashi Y, Kamijo T, Yamamoto M, Murata Y, Phillips JA 3rd, Ogawa M, Seo H.

    Growth Horm IGF Res   Vol. 17 ( 36 ) page: 249-253   2007.6

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  49. Molecular cloning of prostaglandin EP3 receptors from canine sensory ganglia and their facilitatory action on bradykinin-induced mobilization of intracellular calcium Reviewed

    Kozaki Y, Kambe F, Hayashi Y, Ohmori S, Seo H, Kumazawa T, Mizumura K

    J Neurochem   Vol. 100 ( 6 ) page: 1636-1647   2007.3

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  50. Regulation of mesodermal differentiation of mouse embryonic stem cells by basement membranes. Reviewed

    Fujiwara H, Hayashi Y, Sanzen N, Kobayashi R, Weber CN, Emoto T, Futaki S, Niwa H, Murray P, Edgar D, Sekiguchi K.

    J Biol Chem   Vol. 282   page: 29701-29711   2007

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  51. The Sp family of transcription factors regulates the human laminin alpha1 gene in JAR choriocarcinoma cells. Reviewed

    Niimi T, Hayashi Y, Sekiguchi K, Kitagawa Y.

    Biochim Biophys Acta   Vol. 2006 ( 11-12 ) page: 573-579   2006.11

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  52. A novel large-scale production system for modified basement membrane matrices using gene-swapped parietal endoderm cells. Reviewed

    Hayashi Y, Weber CN, Emoto T, Fujiwara H, Sanzen N, Futaki S, Sekiguchi K.

    Matrix Biol   Vol. 25 ( 2 ) page: 85-88   2006.3

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  53. Activation of the RhoB signaling pathway by thyroid hormone receptor ß in thyroid cancer cells. Reviewed

    S. Ichijo, F. Furuya, H. Shimura, Y. Hayashi, K. Takahashi, K. Ohta, T. Kobayashi, K. Kitamura.

    PLoS ONE   Vol. 9 ( 12 ) page: e116252   2005.12

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    DOI: doi: 10.1371/journal.pone.0116252.

  54. Growth hormone releasing hormone receptor (GHRH-r) gene mutation in Indian children with familial isolated growth hormone deficiency: a study from western India Reviewed

    Desai MP, Upadhye PS, Kamijo T, Yamamoto M, Ogawa M, Hayashi Y, Seo H, Nair SR.

    J Pediatr Endocrinol Metab   Vol. 18 ( 10 ) page: 1955-973   2005.10

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  55. The transcriptional landscape of the mammalian genome. Reviewed

    Carninci P et al FANTOM Consortium; RIKEN Genome Exploration Research Group and Genome Science Group (Genome Network Project Core Group).

    Science   Vol. 309   page: 1559-1563   2005.9

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  56. Sox7 Plays Crucial Roles in Parietal Endoderm Differentiation in F9 Embryonal Carcinoma Cells through Regulating Gata-4 and Gata-6 Expression. Reviewed

    S. Futaki, Y. Hayashi, T. Emoto, C.N. Weber, K. SekiguchiS. Futaki, Y. Hayashi, T. Emoto, C.N. Weber, K. Sekiguchi

    Mol Cell Biol   Vol. 24 ( 12 ) page: 10495-10503   2004.12

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  57. A nonsense mutation (E72X) in growth hormone releasing hormone receptor (GHRHR) gene is the major cause of familial isolated growth hormone deficiency in Western region of India: founder effect suggested by analysis of dinucleotide repeat polymorphism close to GHRHR gene Reviewed

    Kamijo, T. Hayashi, Y. Seo, H. Yamamoto, M. Ogawa, M. Choski, C. S. Sawant, N. J. Colaco, M. P. Desai, M. P.

    Growth Horm IGF Res   Vol. 14 ( 5 ) page: 394-401   2004.10

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  58. SOX7 and SOX17 Regulate the Parietal Endoderm-specific Enhancer Activity of Mouse Laminin {alpha}1 Gene Reviewed

    Niimi, T. Hayashi, Y. Futaki, S. Sekiguchi, K.

    J Biol Chem   Vol. 279 ( 36 ) page: 38055-38061   2004.9

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  59. Establishment and characterization of a parietal endoderm-like cell line derived from Engelbreth-Holm-Swarm tumor (EHSPEL), a possible resource for an engineered basement membrane matrix Reviewed

    Hayashi, Y. Emoto, T. Futaki, S. Sekiguchi, K.

    Matrix Biol   Vol. 23 ( 1 ) page: 47-62   2004.4

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  60. Molecular dissection of the alpha -dystroglycan- and integrin-binding sites within the globular domain of human laminin-10 Reviewed

    Ido, H. Harada, K. Futaki, S. Hayashi, Y. Nishiuchi, R. Natsuka, Y. Li, S. Wada, Y. Combs, A. C. Ervasti, J. M. Sekiguchi, K.

    J Biol Chem   Vol. 279 ( 12 ) page: 10946-10954   2004.3

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  61. Molecular basis of constitutive production of basement membrane components: Gene expression profiles of engelbreth-holm-swarm tumor and F9 embryonal carcinoma cells Reviewed

    Futaki, S. Hayashi, Y. Yamashita, M. Yagi, K. Bono, H. Hayashizaki, Y. Okazaki, Y. Sekiguchi, K.

    J Biol Chem   Vol. 278 ( 50 ) page: 50691-50701   2003

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  62. A role for thyroid hormone transporters in transcriptional regulation by thyroid hormone receptors Reviewed

    Ritchie, J. W. Shi, Y. B. Hayashi, Y. Baird, F. E. Muchekehu, R. W. Christie, G. R. Taylor, P. M.

    Mol Endocrinol   Vol. 17 ( 4 ) page: 653-661   2003

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  63. Identification of an upstream enhancer in the mouse laminin alpha 1 gene defining its high level of expression in parietal endoderm cells Reviewed

    Niimi, T. Hayashi, Y. Sekiguchi, K.

    J Biol Chem   Vol. 278 ( 11 ) page: 9332-9338   2003

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  64. Familial isolated growth hormone deficiency: genetics and pathophysiology Invited Reviewed

    Hayashi, Y. Kamijo, T. Ogawa, M. Seo, H.

    Endocr J   Vol. 49 ( 3 ) page: 265-272   2002

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  65. Steroid receptor coactivator-1 deficiency causes variable alterations in the modulation of T(3)-regulated transcription of genes in vivo Reviewed

    Takeuchi, Y. Murata, Y. Sadow, P. Hayashi, Y. Seo, H. Xu, J. O'Malley, B. W. Weiss, R. E. Refetoff, S.

    Endocrinology   Vol. 143 ( 4 ) page: 1346-1352   2002

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  66. Type 1 iodothyronine deiodinase in the house musk shrew (Suncus murinus, Insectivora: Soricidae): cloning and characterization of complementary DNA, unique tissue distribution and regulation by T(3) Reviewed

    Rogatcheva, M. Hayashi, Y. Oda, S. Seo, H. Cua, K. Refetoff, S. Murakami, M. Mori, M. Murata, Y.

    Gen Comp Endocrinol   Vol. 127 ( 1 ) page: 48-58   2002

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  67. Identification and recombinant production of human laminin alpha4 subunit splice variants Reviewed

    Hayashi, Y. Kim, K. H. Fujiwara, H. Shimono, C. Yamashita, M. Sanzen, N. Futaki, S. Sekiguchi, K.

    Biochem Biophys Res Commun   Vol. 299 ( 3 ) page: 498-504   2002

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  68. Increased sensitivity to thyroid hormone in mice with complete deficiency of thyroid hormone receptor alpha Reviewed

    Macchia, P. E. Takeuchi, Y. Kawai, T. Cua, K. Gauthier, K. Chassande, O. Seo, H. Hayashi, Y. Samarut, J. Murata, Y. Weiss, R. E. Refetoff, S.

    Proc Natl Acad Sci U S A   Vol. 98 ( 1 ) page: 349-354   2001

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  69. Somatic gene alteration of AIB1 gene in patients with breast cancer Reviewed

    Shibata, A. Hayashi, Y. Imai, T. Funahashi, H. Nakao, A. Seo, H.

    Endocr J   Vol. 48 ( 2 ) page: 199-204   2001

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  70. Identification of a novel myosin-Va mutation in an ataxic mutant rat, dilute-opisthotonus Reviewed

    Futaki, S. Takagishi, Y. Hayashi, Y. Ohmori, S. Kanou, Y. Inouye, M. Oda, S. Seo, H. Iwaikawa, Y. Murata, Y.

    Mamm Genome   Vol. 11 ( 8 ) page: 649-655   2000

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  71. Involvement of AP-1 and steroidogenic factor (SF)-1 in the cAMP-dependent induction of human adrenocorticotropic hormone receptor (ACTHR) promoter Reviewed

    Sarkar, D. Kambe, F. Hayashi, Y. Ohmori, S. Funahashi, H. Seo, H.

    Endocr J   Vol. 47 ( 1 ) page: 63-75   2000

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  72. Responsiveness to thyroid hormone is enhanced in rat hepatocytes cultured as spheroids compared with that in monolayers: altered responsiveness to thyroid hormone possibly involves complex formed on thyroid hormone response elements Reviewed

    Menjo, M. Yamaguchi, S. Murata, Y. Hayashi, Y. Nagaya, T. Ohmori, S. Refetoff, S. Seo, H.

    Thyroid   Vol. 9 ( 9 ) page: 959-967   1999

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  73. Glucocorticoids increase retinoid-X receptor alpha (RXRalpha) expression and enhance thyroid hormone action in primary cultured rat hepatocytes Reviewed

    Yamaguchi, S. Murata, Y. Nagaya, T. Hayashi, Y. Ohmori, S. Nimura, Y. Seo, H.

    J Mol Endocrinol   Vol. 22 ( 1 ) page: 81-90   1999

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  74. A Japanese family with autosomal dominant growth hormone deficiency Reviewed

    Saitoh, H. Fukushima, T. Kamoda, T. Tanae, A. Kamijo, T. Yamamoto, M. Ogawa, M. Hayashi, Y. Ohmori, S. Seo, H.

    Eur J Pediatr   Vol. 158 ( 8 ) page: 624-627   1999

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  75. 9-cis-retinoic acid decreases the level of its cognate receptor, retinoid X receptor, through acceleration of the turnover Reviewed

    Nomura, Y. Nagaya, T. Hayashi, Y. Kambe, F. Seo, H.

    Biochem Biophys Res Commun   Vol. 260 ( 3 ) page: 729-733   1999

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  76. A novel mutation at the donor splice site of intron 3 of the GH-I gene in a patient with isolated growth hormone deficiency Reviewed

    Hayashi, Y. Kamijo, T. Yamamoto, M. Ohmori, S. Phillips, J. A., 3rd Ogawa, M. Igarashi, Y. Seo, H.

    Growth Horm IGF Res   Vol. 9 ( 6 ) page: 434-437   1999

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  77. Mutations in intron 3 of GH-1 gene associated with isolated GH deficiency type II in three Japanese families Reviewed

    Kamijo, T. Hayashi, Y. Shimatsu, A. Kinoshita, E. Yoshimoto, M. Ogawa, M. Seo, H.

    Clin Endocrinol (Oxf)   Vol. 51 ( 3 ) page: 355-360   1999

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  78. Hereditary isolated growth hormone deficiency caused by GH1 gene mutations in Japanese patients

    Kamijo, T. Hayashi, Y. Seo, H. Ogawa, M.

    Growth Horm IGF Res   Vol. 9 ( suppl B ) page: 31-34   1999

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  79. Inhibition of growth hormone (GH) secretion by a mutant GH-I gene product in neuroendocrine cells containing secretory granules: an implication for isolated GH deficiency inherited in an autosomal dominant manner Reviewed

    Hayashi, Y. Yamamoto, M. Ohmori, S. Kamijo, T. Ogawa, M. Seo, H.

    J Clin Endocrinol Metab   Vol. 84 ( 6 ) page: 2134-2139   1999

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  80. Polymorphism of homopolymeric glutamines in coactivators for nuclear hormone receptors Reviewed

    Hayashi, Y. Yamamoto, M. Ohmori, S. Kikumori, T. Imai, T. Funahashi, H. Seo, H.

    Endocr J   Vol. 46 ( 2 ) page: 279-284   1999

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  81. Selective pituitary resistance to thyroid hormone produced by expression of a mutant thyroid hormone receptor beta gene in the pituitary gland of transgenic mice Reviewed

    Hayashi, Y. Xie, J. Weiss, R. E. Pohlenz, J. Refetoff, S.

    Biochem Biophys Res Commun   Vol. 245 ( 1 ) page: 204-10   1998

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  82. Thyroid hormone action on liver, heart, and energy expenditure in thyroid hormone receptor beta-deficient mice Reviewed

    Weiss, R. E. Murata, Y. Cua, K. Hayashi, Y. Seo, H. Refetoff, S.

    Endocrinology   Vol. 139 ( 12 ) page: 4945-4952   1998

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  83. Molecular cloning of sucrase-isomaltase cDNA in the house musk shrew Suncus murinus and identification of a mutation responsible for isolated sucrase deficiency Reviewed

    Ito, T. Hayashi, Y. Ohmori, S. Oda, S. Seo, H.

    J Biol Chem   Vol. 273 ( 26 ) page: 16464-16469   1998

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  84. A splicing variant of Steroid Receptor Coactivator-1 (SRC-1E): the major isoform of SRC-1 to mediate thyroid hormone action Reviewed

    Hayashi, Y. Ohmori, S. Ito, T. Seo, H.

    Biochem Biophys Res Commun   Vol. 236 ( 1 ) page: 83-87   1997

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  85. Modification of thyroid hormone and 9-cis retinoic acid signaling by overexpression of their cognate receptors using adenoviral vector Reviewed

    Hayashi, Y. Yamaguchi, S. Pohlenz, J. Murata, Y. Refetoff, S. Seo, H.

    Mol Cell Endocrinol   Vol. 131 ( 1 ) page: 59-66   1997

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  86. Tumor suppressor p53 is a negative regulator in thyroid hormone receptor signaling pathways Reviewed

    Bhat, M. K. Yu, C. Yap, N. Zhan, Q. Hayashi, Y. Seth, P. Cheng, S.

    J Biol Chem   Vol. 272 ( 46 ) page: 28989-28983   1997

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  87. Resistance to thyroid hormone caused by two mutant thyroid hormone receptors beta, R243Q and R243W, with marked impairment of function that cannot be explained by altered in vitro 3,5,3'-triiodothyroinine binding affinity Reviewed

    Yagi, H. Pohlenz, J. Hayashi, Y. Sakurai, A. Refetoff, S.

    J Clin Endocrinol Metab   Vol. 82 ( 5 ) page: 1608-1614   1997

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  88. Dominant inheritance of resistance to thyroid hormone not linked to defects in the thyroid hormone receptor alpha or beta genes may be due to a defective cofactor Reviewed

    Weiss, R. E. Hayashi, Y. Nagaya, T. Petty, K. J. Murata, Y. Tunca, H. Seo, H. Refetoff, S.

    J Clin Endocrinol Metab   Vol. 81 ( 12 ) page: 4196-4203   1996

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  89. Resistance to thyrotropin and other abnormalities of the thyrotropin receptor

    Refetoff, S. Sunthornthepvarakul, T. Gottschalk, M. E. Hayashi, Y.

    Recent Prog Horm Res   Vol. 51   page: 97-120   1996

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  90. Thyroxine-binding globulin: organization of the gene and variants Reviewed

    Refetoff, S. Murata, Y. Mori, Y. Janssen, O. E. Takeda, K. Hayashi, Y.

    Horm Res   Vol. 45 ( 3-5 ) page: 128-138   1996

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  91. Expression of thyroid peroxidase in EBV-transformed B cell lines using adenovirus Reviewed

    Hidaka, Y. Hayashi, Y. Fisfalen, M. E. Suzuki, S. Takeda, T. Refetoff, S. DeGroot, L. J.

    Thyroid   Vol. 6 ( 1 ) page: 23-28   1996

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  92. A mouse model of resistance to thyroid hormone produced by somatic gene transfer of a mutant thyroid hormone receptor Reviewed

    Hayashi, Y. Mangoura, D. Refetoff, S.

    Mol Endocrinol   Vol. 10 ( 1 ) page: 100-106   1996

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  93. Studies on the repression of basal transcription (silencing) by artificial and natural human thyroid hormone receptor-beta mutants Reviewed

    Yen, P. M. Wilcox, E. C. Hayashi, Y. Refetoff, S. Chin, W. W.

    Endocrinology   Vol. 136 ( 7 ) page: 2845-2851   1995

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  94. Brief report: resistance to thyrotropin caused by mutations in the thyrotropin-receptor gene Reviewed

    Sunthornthepvarakul, T. Gottschalk, M. E. Hayashi, Y. Refetoff, S.

    N Engl J Med   Vol. 332 ( 3 ) page: 155-160   1995

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  95. Expression of human thyrotropin receptor by recombinant adenovirus Reviewed

    Okamoto, Y. Hayashi, Y. DeGroot, L. J.

    Thyroid   Vol. 5 ( 1 ) page: 51-53   1995

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  96. Do clinical manifestations of resistance to thyroid hormone correlate with the functional alteration of the corresponding mutant thyroid hormone-beta receptors? Reviewed

    Hayashi, Y. Weiss, R. E. Sarne, D. H. Yen, P. M. Sunthornthepvarakul, T. Marcocci, C. Chin, W. W. Refetoff, S.

    J Clin Endocrinol Metab   Vol. 80 ( 11 ) page: 3246-3256   1995

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  97. Molecular cloning of an orphan G-protein-coupled receptor that constitutively activates adenylate cyclase Reviewed

    Eggerickx, D. Denef, J. F. Labbe, O. Hayashi, Y. Refetoff, S. Vassart, G. Parmentier, M. Libert, F.

    Biochem J   Vol. 309 ( 3 ) page: 837-843   1995

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  98. Expression of a thyroid hormone-responsive recombinant gene introduced into adult mice livers by replication-defective adenovirus can be regulated by endogenous thyroid hormone receptor Reviewed

    Hayashi, Y. DePaoli, A. M. Burant, C. F. Refetoff, S.

    J Biol Chem   Vol. 269 ( 39 ) page: 23872-23875   1994

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  99. A new point mutation (C446R) in the thyroid hormone receptor-beta gene of a family with resistance to thyroid hormone Reviewed

    Weiss, R. E. Chyna, B. Duell, P. B. Hayashi, Y. Sunthornthepvarakul, T. Refetoff, S.

    J Clin Endocrinol Metab   Vol. 78 ( 5 ) page: 1253-1256   1994

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  100. Polymorphism of a variant human thyrotropin receptor (hTSHR) gene Reviewed

    Sunthornthepvarakul, T. Hayashi, Y. Refetoff, S.

    Thyroid   Vol. 4 ( 2 ) page: 147-149   1994

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  101. An identical missense mutation in the albumin gene results in familial dysalbuminemic hyperthyroxinemia in 8 unrelated families Reviewed

    Sunthornthepvarakul, T. Angkeow, P. Weiss, R. E. Hayashi, Y. Refetoff, S.

    Biochem Biophys Res Commun   Vol. 202 ( 2 ) page: 781-787   1994

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  102. Resistance to thyroid hormone in subjects from two unrelated families is associated with a point mutation in the thyroid hormone receptor beta gene resulting in the replacement of the normal proline 453 with serine Reviewed

    Refetoff, S. Weiss, R. E. Wing, J. R. Sarne, D. Chyna, B. Hayashi, Y.

    Thyroid   Vol. 4 ( 3 ) page: 249-254   1994

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  103. Mutations of CpG dinucleotides located in the triiodothyronine (T3)-binding domain of the thyroid hormone receptor (TR) beta gene that appears to be devoid of natural mutations may not be detected because they are unlikely to produce the clinical phenotype of resistance to thyroid hormone Reviewed

    Hayashi, Y. Sunthornthepvarakul, T. Refetoff, S.

    J Clin Invest   Vol. 94 ( 2 ) page: 607-615   1994

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  104. Human thyroxine-binding globulin gene: complete sequence and transcriptional regulation Reviewed

    Hayashi, Y. Mori, Y. Janssen, O. E. Sunthornthepvarakul, T. Weiss, R. E. Takeda, K. Weinberg, M. Seo, H. Bell, G. I. Refetoff, S.

    Mol Endocrinol   Vol. 7 ( 8 ) page: 1049-1060   1993

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  105. The relative expression of mutant and normal thyroid hormone receptor genes in patients with generalized resistance to thyroid hormone determined by estimation of their specific messenger ribonucleic acid products Reviewed

    Hayashi, Y. Janssen, O. E. Weiss, R. E. Murata, Y. Seo, H. Refetoff, S.

    J Clin Endocrinol Metab   Vol. 76 ( 1 ) page: 64-69   1993

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  106. Modification of water and electrolyte metabolism during head-down tilting by hypoglycemia in men Reviewed

    Hayashi, Y. Murata, Y. Seo, H. Miyamoto, N. Kambe, F. Ohmori, S. Yamamoto, C. Hayamizu, S. Tamura, Y. Matsui, N.

    J Appl Physiol   Vol. 73 ( 5 ) page: 1785-1790   1992

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MISC 4

  1. Functional adenosine triphosphate-sensitive potassium channel is required in high-carbohydrate diet-induced increase in β-cell mass. Reviewed

    Masatoshi Murase, Yusuke Seino, Ryuya Maekawa, Atsushi Iida, Kaori Hosokawa, Tomohide Hayami, Shin Tsunekawa, Yoji Hamada, Norihide Yokoi, Susumu Seino, Yoshitaka Hayashi, Hiroshi Arima

    Journal of diabetes investigation   Vol. 10 ( 2 ) page: 238 - 250   2019.3

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    AIMS/INTRODUCTION: A high-carbohydrate diet is known to increase insulin secretion and induce obesity. However, whether or not a high-carbohydrate diet affects β-cell mass (BCM) has been little investigated. MATERIALS AND METHODS: Both wild-type (WT) mice and adenosine triphosphate-sensitive potassium channel-deficient (Kir6.2KO) mice were fed normal chow or high-starch (ST) diets for 22 weeks. BCM and the numbers of islets were analyzed by immunohistochemistry, and gene expression levels in islets were investigated by quantitative real-time reverse transcription polymerase chain reaction. MIN6-K8 β-cells were stimulated in solution containing various concentrations of glucose combined with nifedipine and glimepiride, and gene expression was analyzed. RESULTS: Both WT and Kir6.2KO mice fed ST showed hyperinsulinemia and body weight gain. BCM, the number of islets and the expression levels of cyclinD2 messenger ribonucleic acid were increased in WT mice fed ST compared with those in WT mice fed normal chow. In contrast, no significant difference in BCM, the number of islets or the expression levels of cyclinD2 messenger ribonucleic acid were observed between Kir6.2KO mice fed normal chow and those fed ST. Incubation of MIN6-K8 β-cells in high-glucose media or with glimepiride increased cyclinD2 expression, whereas nifedipine attenuated a high-glucose-induced increase in cyclinD2 expression. CONCLUSIONS: These results show that a high-starch diet increases BCM in an adenosine triphosphate-sensitive potassium channel-dependent manner, which is mediated through upregulation of cyclinD2 expression.

    DOI: 10.1111/jdi.12907

    PubMed

  2. Glucose-dependent insulinotropic polypeptide is required for moderate high-fat diet-but not high-carbohydrate diet-induced weight gain Reviewed

    Ryuya Maekawa, Hidetada Ogata, Masatoshi Murase, Norio Harada, Kazuyo Suzuki, Erina Joo, Akiko Sankoda, Atsushi Iida, Takako Izumoto, Shin Tsunekawa, Yoji Hamada, Yutaka Oiso, Nobuya Inagaki, Hiroshi Arima, Yoshitaka Hayashi, Yusuke Seino

    American Journal of Physiology - Endocrinology and Metabolism   Vol. 314 ( 6 ) page: E572 - E583   2018.6

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    Language:English   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)  

    © 2018 American Physiological Society. All rights reserved. Both high-fat (HFD) and high-carbohydrate (ST) diets are known to induce weight gain. Glucose-dependent insulinotropic polypeptide (GIP) is secreted mainly from intestinal K cells upon stimuli by nutrients such as fat and glucose, and it potentiates glucose-induced insulin secretion. GIP is well known to contribute to HFD-induced obesity. In this study, we analyzed the effect of ST feeding on GIP secretion and metabolic parameters to explore the role of GIP in ST-induced weight gain. Both wild-type (WT) and GIP receptor deficient (GiprKO) mice were fed normal chow (NC), ST, or moderate (m)HFD for 22 wk. Body weight was measured, and then glucose tolerance tests were performed. Insulin secretion from isolated islets also was analyzed. WT mice fed ST or mHFD displayed weight gain concomitant with increased plasma GIP levels compared with WT mice fed NC. WT mice fed mHFD showed improved glucose tolerance due to enhanced insulin secretion during oral glucose tolerance tests compared with WT mice fed NC or ST. GiprKO mice fed mHFD did not display weight gain. On the other hand, GiprKO mice fed ST showed weight gain and did not display obvious glucose intolerance. Glucose-induced insulin secretion was enhanced during intraperitoneal glucose tolerance tests and from isolated islets in both WT and GiprKO mice fed ST compared with those fed NC. In conclusion, enhanced GIP secretion induced by mHFD-feeding contributes to increased insulin secretion and body weight gain, whereas GIP is marginally involved in weight gain induced by ST-feeding.

    DOI: 10.1152/ajpendo.00352.2017

    Scopus

    PubMed

  3. *Regulation of amino acid metabolism and alpha cell proliferation by glucagon. Invited Reviewed

    Yoshitaka Hayashi, Yusuke Seino

        2018.1

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    Language:English   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)  

    DOI: 10.1111/jdi.12797

  4. Distribution and hormonal characterization of primary murine L cells throughout the gastrointestinal tract Reviewed

    Suzuki Kazuyo, Iwasaki Kanako, Murata Yuki, Harada Norio, Yamane Shunsuke, Hamasaki Akihiro, Shibue Kimitaka, Joo Erina, Sankoda Akiko, Fujiwara Yuta, Hayashi Yoshitaka, Inagaki Nobuya

    JOURNAL OF DIABETES INVESTIGATION   Vol. 9 ( 1 ) page: 25-32   2018.1

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    Language:English   Publishing type:Rapid communication, short report, research note, etc. (scientific journal)  

    DOI: 10.1111/jdi.12681

    PubMed

Research Project for Joint Research, Competitive Funding, etc. 7

  1. Exploration of humoral factors, which regulate proliferation of islet alpha-cells

    2014.11

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    Grant type:Competitive

  2. 褐色脂肪組織における代謝制御と甲状腺ホルモン感受性調節の相互作用

    2011.12 - 2013.3

    基礎医学研究助成 

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    Grant type:Competitive

  3. 腸管内分泌細胞の腸内環境感知機構

    2011.3

    ダノン学術研究助成金 

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    Grant type:Competitive

  4. グルカゴン遺伝子欠損モデル動物における成長異常発症機構の解明

    2010.6

    ノボ・ノルディスク成長・発達研究賞 

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    Grant type:Competitive

  5. 血糖制御ホルモン遺伝子破壊モデル動物におけるエネルギー代謝異常の解析

    2008.11

    生活習慣病における医学、薬学の萌芽的研究助成 

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    Grant type:Competitive

  6. グルカゴン様ペプチド-1産生細胞の分離ー発生工学的手法を用いて

    2007.9 - 2009.3

    学術研究助成 

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    Grant type:Competitive

  7. プレプログルカゴン遺伝子発現を指標とした膵島発生分化機構の解析

    2006.9

    報彰基金研究奨励 

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    Grant type:Competitive

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KAKENHI (Grants-in-Aid for Scientific Research) 15

  1. グルカゴンによるニコチンアミド代謝制御の生体における意義の解明 研究課題

    2018.4 - 2023.3

    科学研究費補助金  基盤研究(B)

    林 良敬

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    Authorship:Principal investigator 

  2. 糖代謝とアミノ酸代謝のクロストークにおけるグルカゴンの役割の解明

    2015.4 - 2018.3

    科学研究費補助金  基盤研究(B)

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    Authorship:Principal investigator 

  3. 肝臓に由来する膵島α細胞増殖制御因子の同定

    2015.4 - 2017.3

    科学研究費補助金  挑戦的方が研究

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    Authorship:Principal investigator 

  4. 膵島の発生・新生における血管神経ワイヤリング形成とリモデリングの機構の解析

    2013.4 - 2015.3

    科学研究費補助金  新学術領域研究(研究領域提案型)

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    Authorship:Principal investigator 

  5. 褐色脂肪機能制御における内分泌系とサーチュインのクロストーク

    2012.4 - 2015.3

    科学研究費補助金  挑戦的萌芽研究

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    Authorship:Principal investigator 

  6. 膵島の発生・新生における血管神経ワイヤリング形成とリモデリングの機構の解析

    2011.4 - 2013.3

    科学研究費補助金  新学術領域研究、課題番号:23122507

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    Authorship:Principal investigator 

  7. 腸管上皮・内分泌細胞をターゲットとした新規治療戦略の創出

    2009.4 - 2012.3

    科学研究費補助金  挑戦的萌芽研究

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    Authorship:Principal investigator 

  8. 脳・神経系における甲状腺ホルモン作用の解析ー海馬などにおける新規標的遺伝子の探索

    2006.4 - 2008.3

    科学研究費補助金  基盤研究(C)

    林 良敬

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    Authorship:Principal investigator 

  9. 栄養素感知に関わる腸管内分泌ホルモン分泌機構の統合的解析

    Grant number:20H03731  2020.4 - 2023.3

    科学研究費助成事業  基盤研究(B)

    稲垣 暢也, 原田 範雄, 林 良敬, 山根 俊介

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    1) K細胞・L細胞・I細胞の腸管内局在および重複の検討
    2) K細胞・L細胞・I細胞の発現プロファイル比較と高発現遺伝子・タンパクの機能解析
    3) 胆汁によるGIP, GLP-1の分泌制御についての検討
    申請者らは脂肪摂取時GIP分泌に腸管内の胆汁が必須であることを報告しており、この機序の詳細に関して検討する。
    4) GPR120-CCK-GIP経路が全身の代謝状態におよぼす影響
    腸管内のGPR120シグナルの減弱はCCK分泌の低下を介してGIPの過分泌を抑制し、高脂肪食摂取による肥満・インスリン抵抗性を緩和できる可能性があり、腸管上皮特異的GPR120欠損マウスを作製し、表現型を評価する。
    マウスのCckプロモーターを活性化して赤色蛍光タンパク質tdTomato(Tomato)を産生するCCKレポーターマウスを作製した。フローサイトメーターによる解析では小腸上部、小腸下部、大腸の上皮細胞におけるTomato陽性細胞の比率は、それぞれ0.95、0.54、0.06%であった。脂肪酸受容体Gpr120、Gpr40、Gpr43、オレオイルエタノールアミド受容体Gpr119は、小腸から単離したTomato陽性細胞で高い発現を示したが、大腸のTomato陽性細胞では発現が見られなかった。グルコースおよびフルクトースの輸送体であるSglt1, Glut2, Glut5は、Tomato陽性細胞と陰性細胞の両方で発現していたが、これらの輸送体のTomato陽性細胞における発現量は、小腸上部から大腸にかけて減少する傾向が見られた。ペプチド輸送体Pept1とペプチド受容体Gpr93はTomato陽性細胞と陰性細胞の両方に発現していたが、カルシウム感知受容体(Casr)は小腸のTomato陽性細胞にのみ発現していた。このような結果からI細胞の数やI細胞での遺伝子発現が、消化管の部位によって異なることが明らかになった(Kato T, et al.J Mol Endocrinol. 2021 )。
    GLP-1産生L細胞で高い発現を認める炭酸脱水酵素8 (carbonic anhydrase 8: Car8) に関して、腸管内分泌細胞株STC-1 を用いて、Car8 の発現抑制により長鎖脂肪酸刺激によるGLP-1 分泌が増強、過剰発現により減弱すること、さらにCar8 欠損マウスのコーン油負荷後GLP-1分泌は野生型マウスに比べて有意に高値であることも見出し報告した(Fujiwara et al.Am J Physiol Gastrointest Liver Physiol.2021)。
    CCK産生I細胞可視化マウス(CCK-tdTomatoマウス)を確立し、このマウスを用いてI細胞の腸管内局在に関して得られた知見を既に論文として報告した。またL細胞可視化マウス(Gcg-GFPノックインマウス) の小腸から単離したL細胞の解析により、炭酸脱水酵素8(Car8)がL細胞で発現していることを見出し、Car8が細胞内カルシウム動態の制御を介してGLP-1分泌調節に関与することも報告した。K細胞可視化マウス(GIP-GFPノックインマウス) とL細胞可視化マウスおよび今回新たに作製したI細胞可視化マウスを用いて、それぞれの分泌細胞の位置的関係や重複についての解析に着手している。令和2年度に予定していた計画としてはおおむね順調に進捗しているものと判断している。
    計画に従って、K細胞、L細胞、I細胞、GIP/CCK共陽性細胞(K/I細胞)およびGLP1/CCK共陽性細胞(L/I細胞) ぞれぞれに特異的に発現する遺伝子を同定し、遺伝子欠損マウスを用いた解析などにより、GIP、GLP-1、CCK分泌機序への関与を明らかにする。また胆汁酸による腸管内分泌ホルモン分泌への影響の検討にも着手する。さらには腸管におけるGPR120シグナルの生理的意義を検討するため、腸管上皮特異的GPR120欠損マウスを作製し、表現型を評価する。各研究者は計画の遂行にあたり必要な技術に熟達しており、研究代表者の統括のもと、相互の連携により組織的に研究を実施する。

  10. Glucagon-dependent autonomic regulatory system and its role in cardiovascular disease

    Grant number:19H03651  2019.4 - 2022.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Coinvestigator(s) 

  11. Regulation of nicotinamide metabolism by glucagon

    Grant number:18H03176  2018.4 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (B)

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    Authorship:Principal investigator 

    Grant amount:\17160000 ( Direct Cost: \13200000 、 Indirect Cost:\3960000 )

  12. Analysis of the mechanisms of incretin secretion: similarities or differences of GIP and GLP-1 secretion

    Grant number:16H05326  2016.4 - 2019.3

    Inagaki Nobuya

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    Authorship:Coinvestigator(s) 

    We examined FABP5 localization in K-cells before and after oral fat
    intake using electron microscopy and confocal microscopy.FABP5 was present both in the nucleus and in the cytoplasm of K-cells before loading. In contrast, 60 minutes after fat loading, the intranuclear localization has disappeared. The expression of Regulator of G protein signaling 4(RGS4) was found to be elevated in the K cells of FABP5-deficient mouse. We clarified that both GPR120 and GPR40 are involved in GIP secretion after fat intake. It is suggested that GPR40 expressed in K-cells directly sense LCFA, and in contrast, GPR120 expressed in I-cells indirectly regulates GIP secretion in the presence of bile via CCK secretion.We also obtained results suggesting that carbonic anhydrase 8 (Car8), which shows high expression in L-cells, negatively regulates GLP-1 secretion in response to fatty acid stimulation.

  13. Exploration of the role of glucagon in the crosstalk between glucose metabolism and amino acid metabolism

    Grant number:15H04681  2015.4 - 2019.3

    Hayashi Yoshitaka

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    Authorship:Principal investigator 

    Grant amount:\14950000 ( Direct Cost: \11500000 、 Indirect Cost:\3450000 )

    Glucagon was originally reported as a hyperglycemic substance that is present in pancreatic extract in 1923, the year of the Nobel Prize in Physiology or Medicine given to discovery of insulin. Therefore, the major physiological role of glucagon has been considered to raise blood glucose levels. However, mice deficient in glucagon gene, which we have produced, are normoglycemic and displayed increased serum amino acid levels. Our effort has been made to explore mechanisms involved in glucagon-dependent regulation of amino acid metabolism. Several lines of data and evidence are indicating that “truly” specific and physiological role of glucagon is regulation of amino acid catabolism, especially conversion of amino acids to substrates available for gluconeogenesis.

  14. Identification of a liver-derived factor that regulates proliferation of islet alpha cells

    Grant number:15K15356  2015.4 - 2018.3

    Hayashi Yoshitaka

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    Authorship:Principal investigator 

    Grant amount:\3640000 ( Direct Cost: \2800000 、 Indirect Cost:\840000 )

    Homozygous glucagon-GFP knock-in mice, which is deficient in all the peptide derived from proglucagon, are nomoglycemic and develop hyperplasia of GFP-positive islet alpha-like cells. To elucidate the regulatory mechanisms of alpha cell proliferation, subrenal capsule transplantation experiments were performed, and the result indicated that proliferation of alpha cells is regulated by a humoral factor(s) derived from liver. Although exploration to identify a specific humoral factor failed to identify such factors encoded by a specific gene, accumulating data suggested that hyperaminoacidemia under glucagon deficiency should be involved in accelerated proliferation of islet alpha cells.

  15. Development of treatment and prevention for pancreatic neuroendocrine tumor using glucagon gene knock-out mouse

    Grant number:15K10049  2015.4 - 2018.3

    Kikumori Toyone

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    Authorship:Collaborating Investigator(s) (not designated on Grant-in-Aid) 

    We explored treatment / prevention methods by clarifying pathologically and molecular biologically the biological properties of tumor developed in glucagon gene knockout mouse in which pancreatic tumors arose spontaneously. We reported that the pancreatic tumor developed in this mouse closely resembles histological and biological attitudes to human pancreatic neuroendocrine tumor (pNET). Expression of cellular growth factors and angiogenic factors (AKT, mTOR, VEGF, etc.) in pancreatic islet cells at each stage of the process of malignant transformation were examined by immunostaining of excised tissue specimens. Everolimus, an mTOR inhibitor which has clinical indication for pNET, was continued to be administered to these mice shortly after birth, and it was confirmed that pancreatic tumor development was suppressed.

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Industrial property rights 2

  1. タンパク質の生産に用いることが可能なF9胚性腫瘍細胞、およびその利用

    林良敬 関口清俊

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    Applicant:独立行政法人 科学技術振興機構

    Application no:2005-130194  Date applied:2005.4

    Announcement no:2006-304668 

    Country of applicant:Domestic  

  2. 壁側内胚葉様細胞株、及びその樹立方法、並びに分泌蛋白の製造方法

    林良敬 関口清俊

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    Applicant:独立行政法人 科学技術振興機構

    Application no:2002-301052  Date applied:2002.10

    Announcement no:2004-135519 

    Country of applicant:Domestic  

 

Teaching Experience (On-campus) 8

  1. 内分泌・代謝学セミナー

    2021

  2. 基礎医学セミナー

    2021

  3. 基礎医学セミナー

    2020

  4. 内分泌・代謝学セミナー

    2020

  5. 基礎医学セミナー

    2019

  6. 内分泌・代謝学セミナー

    2019

  7. 自然環境と人間

    2010

  8. 「自然環境と人間」

    2009

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