Updated on 2024/02/20

写真a

 
TAKAHASHI Koji
 
Organization
Graduate School of Science Assistant Professor
Graduate School
Graduate School of Science
Undergraduate School
School of Science
Title
Assistant Professor
Contact information
メールアドレス
External link

Degree 3

  1. Doctor of Agriculture ( 1997.3   Nagoya University ) 

  2. 修士(理学) ( 1993.3   東京大学 ) 

  3. 学士(理学) ( 1991.3   静岡大学 ) 

Research Interests 1

  1. plant growth regulation

Research Areas 1

  1. Others / Others  / Plant Physiology

Current Research Project and SDGs 2

  1. オーキシン誘導性伸長生長の分子生理学的研究

  2. Study on Growth Regulation and Cell Wall Proteins in Plant

Education 3

  1. Nagoya University   Graduate School, Division of Agriculture

    - 1996.3

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    Country: Japan

  2. The University of Tokyo   Graduate School, Division of Science

    1991.4 - 1993.3

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    Country: Japan

  3. Shizuoka University   Faculty of Science   Biology

    1987.4 - 1991.3

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    Country: Japan

Professional Memberships 2

  1. The Japanese Society of Plant Physiologists

  2. The Botanical Society of Japan

Committee Memberships 3

  1. 日本植物生理学会 運営委員会   広報幹事  

    2020.3 - 2022.3   

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    Committee type:Academic society

  2. 日本植物学会 第84回大会 実行委員会   オンライン発表担当代表  

    2020.9   

  3. 第60回 日本植物生理学会年会 大会実行委員会   会場担当代表  

    2019.3   

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    Committee type:Academic society

 

Papers 37

  1. Phosphorylation of plasma membrane H+-ATPase Thr881 participates in light-induced stomatal opening Reviewed

    Yuki Hayashi, Kohei Fukatsu, Koji Takahashi, Satoru N. Kinoshita, Kyohei Kato, Taku Sakakibara, Keiko Kuwata & Toshinori Kinoshita

    Nature Communications   Vol. 15   page: 1194   2024.2

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1038/s41467-024-45248-5

  2. Identification and improvement of isothiocyanate-based inhibitors on stomatal opening to act as drought tolerance-conferring agrochemicals Reviewed

    Yusuke Aihara, Bumpei Maeda, Kanna Goto, Koji Takahashi, Mika Nomoto, Shigeo Toh, Wenxiu Ye, Yosuke Toda, Mami Uchida, Eri Asai, Yasuomi Tada, Kenichiro Itami, Ayato Sato, Kei Murakami* and Toshinori Kinoshita

    Nature Communications   Vol. 14   page: 2665   2023.5

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1038/s41467-023-38102-7

  3. Tryptophan synthase ß subunit 1 affects stomatal phenotypes in Arabidopsis thaliana Reviewed

    Midori N Soda, Yuki Hayashi, Koji Takahashi, Toshinori Kinoshita

    Frontiers in Plant Science   Vol. 13   page: 1011360   2022.11

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.3389/fpls.2022.1011360

  4. ABP1-TMK auxin perception mediates ultrafast global phosphorylation and auxin canalization. Reviewed International coauthorship

    Jiří Friml, Michelle Gallei, Zuzana Gelová, Alexander Johnson, Ewa Mazur, Aline Monzer, Lesia Rodriguez, Mark Roosjen, Inge Verstraeten, Branka Živanović, Minxia Zou, Lukas Fiedler, Caterina Giannini, Peter Grones, Walter Kaufmann, Andre Kuhn, Madhumitha Narasimhan, Marek Randuch, Nikola Rydza, Koji Takahashi, Shutang Tan, Anastasia Teplova, Toshinori Kinoshita, Dolf Weijers, Hana Rakusova.

    Nature   Vol. 609   page: 575 - 581   2022.9

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1038/s41586-022-05187-x

  5. Identification of stomatal-regulating molecules from de novo arylamine collection through aromatic C-H amination. Reviewed

    Yosuke Toda, Gregory J.P. Perry, Shimpei Inoue, Eri Ito, Takahiro Kawakami, Mina R. Narouz, Koji Takahashi, Yusuke Aihara, Bumpei Maeda, Toshinori Kinoshita, Kenichiro Itami, Kei Murakami

    Scientific Reports   Vol. 12   page: 949   2022.1

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: https://doi.org/10.1038/s41598-022-04947-z

  6. Identification of ABA-dependent phosphorylated bHLH transcription factors in guard cells of Vicia faba by mass spectrometry. Reviewed

    Yuki Hayashi, Yohei Takahashi, Kohei Fukatsu, Yasuomi Tada, Koji Takahashi, Keiko Kuwata, Takamasa Suzuki, Toshinori Kinoshita

    Frontiers in Plant Science   Vol. 12   page: 735271   2021.12

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3389/fpls.2021.735271

  7. Cell surface and intracellular auxin signalling for H<SUP>+</SUP> fluxes in root growth

    Li, LX; Verstraeten, I; Roosjen, M; Takahashi, K; Rodriguez, L; Merrin, J; Chen, J; Shabala, L; Smet, W; Ren, H; Vanneste, S; Shabala, S; De Rybel, B; Weijers, D; Kinoshita, T; Gray, WM; Friml, J

    NATURE   Vol. 599 ( 7884 ) page: 273 - +   2021.11

  8. TMK-based cell-surface auxin signalling activates cell-wall acidification

    Lin, WW; Zhou, X; Tang, WX; Takahashi, K; Pan, X; Dai, JW; Ren, H; Zhu, XY; Pan, SQ; Zheng, HY; Gray, WM; Xu, TD; Kinoshita, T; Yang, ZB

    NATURE   Vol. 599 ( 7884 ) page: 278 - +   2021.11

  9. A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair Reviewed

      Vol. 48 ( 18 )   2020.10

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/nar/gkaa748

    Web of Science

    PubMed

  10. Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization Reviewed

    Oochi Akihiro, Hajny Jakub, Fukui Kosuke, Nakao Yukio, Gallei Michelle, Quareshy Mussa, Takahashi Koji, Kinoshita Toshinori, Harborough Sigurd Ramans, Kepinski Stefan, Kasahara Hiroyuki, Napier Richard, Friml Jiri, Hayashi Ken-ichiro

    PLANT PHYSIOLOGY   Vol. 180 ( 2 ) page: 1152 - 1165   2019.6

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1104/pp.19.00201

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    PubMed

  11. Brassinosteroid Induces Phosphorylation of the Plasma Membrane H+-ATPase during Hypocotyl Elongation in Arabidopsis thaliana Reviewed

    Minami Anzu, Takahashi Koji, Inoue Shin-ichiro, Tada Yasuomi, Kinoshita Toshinori

    PLANT AND CELL PHYSIOLOGY   Vol. 60 ( 5 ) page: 935 - 944   2019.5

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    Language:Japanese   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcz005

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  12. A Super Strong Engineered Auxin-TIR1 Pair Reviewed

    Yamada Ryotaro, Murai Keiichiro, Uchida Naoyuki, Takahashi Koji, Iwasaki Rie, Tada Yasuomi, Kinoshita Toshinori, Itami Kenichiro, Torii Keiko U., Hagihara Shinya

    PLANT AND CELL PHYSIOLOGY   Vol. 59 ( 8 ) page: 1538 - 1544   2018.8

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcy127

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    PubMed

  13. Rapid and reversible root growth inhibition by TIR1 auxin signalling Reviewed

    Fendrych Matyas, Akhmanova Maria, Merrin Jack, Glanc Matous, Hagihara Shinya, Takahashi Koji, Uchida Naoyuki, Torii Keiko U., Friml Jiri

    NATURE PLANTS   Vol. 4 ( 7 ) page: 453 - 459   2018.7

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/s41477-018-0190-1

    Web of Science

    PubMed

  14. Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair. Reviewed

    Naoyuki Uchida, Koji Takahashi, Rie Iwasaki, Ryotaro Yamada, Masahiko Yoshimura, Takaho A Endo, Seisuke Kimura, Hua Zhang, Mika Nomoto, Yasuomi Tada, Toshinori Kinoshita, Kenichiro Itami, Shinya Hagihara, Keiko U Torii

    Nature Chemical Biology   Vol. 14 ( 3 ) page: 299 - +   2018.3

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1038/NCHEMBIO.2555

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    PubMed

  15. Auxin influx carrier AUX1 confers acid resistance for Arabidopsis root elongation through the regulation of plasma membrane H+-ATPase Reviewed

    Shin-ichiro Inoue, Koji Takahashi, Hiromi Okumura-Noda, Toshinori Kinoshita

    Plant and Cell Physiology   Vol. 57 ( 10 ) page: 2194-2201   2016

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcw136

  16. A flowering integrator, SOC1, affects stomatal opening in Arabidopsis thaliana. Reviewed

    Kimura Y, Aoki S, Ando E, Kitatsuji A, Watanabe A, Ohnishi M, Takahashi K, Inoue S, Nakamichi N, Tamada Y, Kinoshita T

    Plant & Cell Physiology   Vol. 56 ( 4 ) page: 640-649   2015.4

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcu214

  17. Abscisic acid suppresses hypocotyl elongation by dephosphorylating plasma membrane H+-ATPase in Arabidopsis thaliana Reviewed

    Yuki Hayashi, Koji Takahashi, Shin-ichiro Inoue and Toshinori Kinoshita

    Plant and Cell Physiology     2014

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1093/pcp/pcu028

  18. Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana Reviewed

    Masakazu Tomiyama, Shin-ichiro Inoue, Tomo Tsuzuki, Midori Soda, Sayuri Morimoto, Yukiko Okigaki, Takaya Ohishi, Nobuyoshi Mochizuki, Koji Takahashi, Toshinori Kinoshita

    Journal of Plant Research   Vol. 127   page: 553-563   2014

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.1007/s10265-014-0636-0

  19. Overexpression of the Mg-chelatase H subunit in guard cells confers drought tolerance via promotion of stomatal closure in Arabidopsis thaliana Reviewed

    Tomo Tsuzuki, Koji Takahashi, Masakazu Tomiyama, Shin-ichiro Inoue and Toshinori Kinoshita

    Frontiers in Plant Science     2013.10

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    Language:English   Publishing type:Research paper (scientific journal)  

    DOI: 10.3389/fpls.2013.00440

  20. Evolutionary appearance of the plasma membrane H+-ATPase containing a penultimate threonine in the bryophyte Reviewed

    Masaki Okumura, Koji Takahashi, Shin-ichiro Inoue, Toshinori Kinoshita

    Plant Signaling & Behavior   Vol. 7 ( 8 ) page: 1-4   2012.8

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    Language:English   Publishing type:Research paper (scientific journal)  

  21. Characterization of the plasma membrane H+-ATPase in the liverwort Marchantia polymorpha Reviewed

    Masaki Okumura, Shin-ichiro Inoue, Koji Takahashi, Kimitsune Ishizaki, Takayuki Kohchi, Toshinori Kinoshita

    Plant Physiology   Vol. 159   page: 826-834   2012.6

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    Language:English   Publishing type:Research paper (scientific journal)  

  22. Auxin activates the plasma membrane H+-ATPase by phosphorylation during hypocotyl elongation in Arabidopsis thaliana Reviewed

    Koji Takahashi, Ken-ichiro Hayashi, Toshinori Kinoshita

    Plant Physiology   Vol. 159   page: 632-641   2012.6

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  23. Immunohistochemical detection of blue light-induced phosphorylation of the plasma membrane H+-ATPase in stomatal guard cells. Reviewed

    Hayashi M, Inoue S, Takahashi K, Kinoshita T

    Plant and Cell Physiology   Vol. 52   page: 1238-1248   2011

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    Language:English   Publishing type:Research paper (scientific journal)  

  24. Mg-chelatase H subunit affects ABA signaling in stomatal guard cells, but is not an ABA receptor in Arabidopsis thaliana. Reviewed

    Tsuzuki T, Takahashi K, Inoue S, Okigaki Y, Tomiyama M, Hossain MA, Shimazaki K, Murata Y, Kinoshita T

    Journal of Plant Research   Vol. 124   page: 527-538   2011

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    Language:English   Publishing type:Research paper (scientific journal)  

  25. Wall-yielding properties of cell walls from elongating cucumber hypocotyls in relation to the action of expansin Reviewed

    Takahashi K, Hirata S, Kido N, and Katou K

    Plant and Cell Physiology   Vol. 47 ( 11 ) page: 1520-1529   2006

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  26. The Role of wall Ca<SUP>2+</SUP> in the regulation of wall extensibility during the acid-induced extension of soybean hypocotyl cell walls Reviewed

    Naofumi Ezaki, Nobuo Kido, Koji Takahashi and Kiyoshi Katou

    Plant and Cell Physiology   Vol. 46 ( 11 ) page: 1831-1838   2005

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    Language:English   Publishing type:Research paper (scientific journal)  

  27. Distribution of yieldin, a regulatory protein of the cell wall yield threshold, in etiolated cowpea seedlings. Reviewed

    Okamoto-Nakazato A, Takahashi K, Katoh-Semba R, Katou K

    Plant and Cell Physiology   Vol. 42 ( 9 ) page: 952-958   2001

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    Language:English   Publishing type:Research paper (scientific journal)  

  28. Sugar-induced increase in cytosolic Ca<SUP>2+</SUP> in Arabidopsis thaliana whole plants. Reviewed

    Furuichi T, Mori IC, Takahashi K, Muto S.

    Plant and Cell Physiology   Vol. 42 ( 10 ) page: 1149-1155   2001

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    Language:English   Publishing type:Research paper (scientific journal)  

  29. Molecular cloning of yieldins regulating the yield threshold of cowpea cell walls: cDNA cloning and characterization of recombinant yieldin Reviewed

    Akane Okamoto-Nakazato, Koji Takahashi, Nobuo Kido, Katsushi Owaribe, and Kiyoshi Katou

    Plant, Cell and Environment   Vol. 23 ( 2 ) page: 155-164   2000

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    Language:English   Publishing type:Research paper (scientific journal)  

  30. A calcium-dependent protein kinase functions in wound healing in Ventricaria ventricosa (Chlorophyta) Reviewed

    Koh-ichi Sugiyama, Izumi C. Mori, Koji Takahashi, Shoshi Muto and Ikuko Shihira-Ishikawa

    Journal of Phycology   Vol. 36 ( 6 ) page: 1145-1152   2000

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    Language:English   Publishing type:Research paper (scientific journal)  

  31. Salicylic acid induces extracellular superoxide generation follows by an increase in cytosolic calcium ion in tobacco suspension culture : the earliest events in salicylic acid signal transduction Reviewed

    Tomonori Kawano, Nobuya Sahashi, Koji Takahashi, Nobuyuki Uozumi and Shoshi Muto

    Plant and Cell Physiology   Vol. 39 ( 7 ) page: 721-730   1998

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    Language:English   Publishing type:Research paper (scientific journal)  

  32. Calcium chelator and channel blockers supress the IAA-induced membrane hyperpolarization without inhibiting the following growth promotion in hypocotyl sections of Vigna unguiculata under xylem perfusion Reviewed

    Koji Yuasa, Koji Takahashi and Kiyoshi Katou

    Plant and Cell Physiology   Vol. 39 ( 9 ) page: 978-986   1998

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    Language:English   Publishing type:Research paper (scientific journal)  

  33. Mastoparan induces an increase in cytosolic calcium ion concentration and subsequent activation of protein kinases in tobacco suspension culture cells Reviewed

    Koji Takahashi, Minoru Isobe and Shoshi Muto

    Biochimica et Biophysica Acta-Molecular Cell Research   Vol. 1401 ( 3 ) page: 339-346   1998

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  34. An increase in cytosolic calcium ion concentration precedes hypoosmotic shock-induced activation of protein kinases in tobacco suspension culture cells Reviewed

    Koji Takahashi, Minoru Isobe and Shoshi Muto

    FEBS Letters   Vol. 401 ( 2-3 ) page: 202-206   1997

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  35. Hypoosmotic shock induces increases in cytosolic Ca2+ in tobacco suspension-culture cells Reviewed

    Koji Takahashi, Minoru Isobe, Marc R. Knight, Anthony J. Trewavas and Shoshi Muto

    Plant Physiology   Vol. 113 ( 2 ) page: 587-594   1997

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  36. Purification and characterization of an algal (Dunaliella tertiolecta) protein cross-reacting with an anti-Gα-common antibody Reviewed

    Koji Takahashi, Takashi Yuasa and Shoshi Muto

    Physiologia Plantarum   Vol. 94 ( 3 ) page: 486-490   1995

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    Authorship:Lead author   Language:English   Publishing type:Research paper (scientific journal)  

  37. Purification and characterization of a Ca<SUP>2+</SUP>-dependent protein kinase from the halotolerant green alga Dunaliella tertiolecta. Reviewed

    Takashi Yuasa, Koji Takahashi and Shoshi Muto

    Plant and Cell Physiology   Vol. 36 ( 4 ) page: 699-708   1995

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Books 2

  1. テイツ/ザイガー植物生理学・発生学 原著第6版 第3章 水と植物細胞、第4章 植物における水収支

    木下俊則、高橋宏二(第3章、第4章)( Role: Joint translator)

    講談社サイエンティフィック  2017.2 

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    Language:Japanese Book type:Textbook, survey, introduction

  2. The regulation of plant cell expansion: Auxin-induced turgor-driven cell elongation. In "Molecular Cell Biology of the Growth and Differentiation of Plant Cells"

    Koji Takahashi, Toshinori Kinoshita( Role: Joint author)

    CRC Press  2016 

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    Language:English Book type:Scholarly book

MISC 5

  1. オーキシン応答の自在操作を実現する人工ホルモン・受容体ペアの創成

    高橋宏二、萩原伸也、鳥居啓子、打田直行

    化学と生物   Vol. 57 ( 2 ) page: 80 - 87   2019

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    Authorship:Lead author  

  2. Harnessing Synthetic Chemistry to Probe and Hijack Auxin Signaling

    Keiko U. Torii, Naoyuki Uchida, Koji Takahashi, Shinya Hagihara

    New Phytologist.   Vol. 220 ( 2 ) page: 417 - 424   2018.8

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    Publishing type:Article, review, commentary, editorial, etc. (scientific journal)  

  3. 植物細胞壁の臨海降伏張力を制御するタンパク質yieldin オーキシンによる酸成長を支える新機能タンパク質

    高橋宏二、中里(岡本)朱根、加藤潔

    化学と生物   Vol. 39 ( 1 ) page: 9 - 10   2001

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    Authorship:Lead author  

  4. エクオリンを用いた高等植物の細胞内カルシウムの測定

    高橋宏二、森泉、武藤尚志

    化学と生物   Vol. 36 ( 1 ) page: 66 - 70   1998

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    Authorship:Lead author  

  5. 浸透圧調節とプロテインキナーゼ

    武藤尚志、湯浅高志、高橋宏二

    現代化学   Vol. 増刊30   page: 146 - 151   1996

Presentations 15

  1. 気孔孔辺細胞に特異的に発現する遺伝子の探索

    小屋翔太、小野奈津子、高橋宏二、木下俊則

    第76回日本植物学会 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:兵庫県立大学   Country:Japan  

  2. 進化的視点からみた苔類ゼニゴケの細胞膜プロトンポンプ

    奥村将樹、井上晋一郎、高橋宏二、石崎公庸、河内孝之、木下俊則

    第76回日本植物学会 

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    Event date: 2012.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:兵庫県立大学   Country:Japan  

  3. Auxin activates the plasma membrane H+-ATPase by phosphorylation of the penultimate threonine during hypocotyl elongation in Arabidopsis thaliana International conference

    Takahashi K, Hayashi K, Kinoshita T

    23rd International Conference on Arabidopsis Research 

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    Event date: 2012.7

    Language:English   Presentation type:Poster presentation  

    Country:Austria  

  4. Mg-キラターゼHサブユニットの孔辺細胞のABAシグナル伝達への関与

    都築朋、高橋宏二、井上晋一郎、富山将和、村田芳行、島崎研一郎、木下俊則

    第53回日本植物生理学会 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  5. オーキシン誘導性伸長生長における細胞膜プロトンポンプの活性制御

    高橋宏二、林謙一郎、木下俊則

    第53回日本植物生理学会 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都産業大学   Country:Japan  

  6. 苔類ゼニゴケにおける細胞膜プロトンポンプの同定と活性化機構の解析

    奥村将樹、井上晋一郎、高橋宏二、石崎公庸、河内孝之、木下俊則

    第53回日本植物生理学会 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  7. 免疫組織学的手法による孔辺細胞細胞膜H+-ATPaseの青色光に依存したリン酸化の検出

    林真妃、井上晋一郎、高橋宏二、木下俊則

    第53回日本植物生理学会 

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    Event date: 2012.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:京都産業大学   Country:Japan  

  8. オーキシン誘導性伸長生長への細胞膜プロトンポンプの関与

    高橋宏二、木下俊則

    第75回日本植物学会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  9. 苔類ゼニゴケにおける細胞膜プロトンポンプの探索および機能解析

    奥村将樹、井上晋一郎、石崎公庸、河内孝之、高橋宏二、木下俊則

    第75回日本植物学会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Country:Japan  

  10. 青色光に依存した孔辺細胞細胞膜H+-ATPaseリン酸化の免疫組織学的検出を用いた気孔開閉シグナル伝達の解明

    林真妃、井上晋一郎、高橋宏二、木下俊則

    第75回日本植物学会 

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    Event date: 2011.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:東京大学   Country:Japan  

  11. 苔類ゼニゴケにおける細胞膜プロトンポンプの構造および機能解析 International conference

    奥村将樹、井上晋一郎、高橋宏二、石崎公庸、河内孝之、木下俊則

    第52回日本植物生理学会 

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    Event date: 2011.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:要旨集   Country:Japan  

  12. オーキシンによるシロイヌナズナ胚軸伸長促進機構の解析

    高橋宏二、木下俊則

    第52回日本植物生理学会 

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    Event date: 2011.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:要旨集   Country:Japan  

  13. 免疫組織染色による青色光に依存した孔辺細胞細胞膜H+-ATPaseのリン酸化の検出

    林真妃、井上晋一郎、高橋宏二、木下俊則

    第52回日本植物生理学会 

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    Event date: 2011.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:要旨集   Country:Japan  

  14. アブシジン酸非感受性の新奇気孔開度変異体rtl1の解析

    都築 朋、 高橋宏二、 井上晋一郎、 沖垣友季子、 村田芳行、 島崎研一郎、木下俊則

    第52回日本植物生理学会 

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    Event date: 2011.3

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:要旨集   Country:Japan  

  15. アブシジン酸非感受性の新奇気孔開度変異体rtl1の単離と表現型解析

    都築 朋、沖垣友季子、高橋宏二、木下俊則

    第74回日本植物学会 

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    Event date: 2010.9

    Language:Japanese   Presentation type:Oral presentation (general)  

    Venue:中部大学(春日井)   Country:Japan  

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KAKENHI (Grants-in-Aid for Scientific Research) 5

  1. オーキシン共受容体の特異的相互作用を実現するタンパク質改変と酸成長誘導機構の解明

    2020.4 - 2023.3

    科学研究費補助金  基盤研究(C)

    高橋 宏二

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    Authorship:Principal investigator 

  2. Modification of auxin receptors and mechanism of acid growth

    Grant number:20K06685  2020.4 - 2023.3

    Grants-in-Aid for Scientific Research  Grant-in-Aid for Scientific Research (C)

    Takahashi Koji

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    Authorship:Principal investigator 

    Grant amount:\4290000 ( Direct Cost: \3300000 、 Indirect Cost:\990000 )

    To artificially control the intracellular signal transduction of the phytohormone auxin, which mediates various physiological actions such as morphogenesis, growth regulation, and environmental response throughout the life cycle of plants, I have developed novel auxin research tools by modifying auxin receptor TIR1/AFB and the degron sequences of co-receptor Aux/IAA. In addition, I have clarified the mechanism of auxin-induced plant cell elongation (acid growth), which is a representative physiological action of auxin.

  3. 細胞膜プロトンポンプ活性のライブイメージング技術の確立と応用

    2017.4 - 2020.3

    科学研究費補助金  基盤研究(C)

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    Authorship:Principal investigator 

  4. Live-imaging of PM H+-ATPase activity

    Grant number:17K07443  2017.4 - 2020.3

    Takahashi Koji

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    Authorship:Principal investigator 

    Grant amount:\4940000 ( Direct Cost: \3800000 、 Indirect Cost:\1140000 )

    To visualize the plasma membrane H+-ATPase activity, I tried to produce the bio-image sensor using fluorescence resonance energy transfer (FRET) or circulary permutated fluorescent protein which can detect the bimolecular interaction between plasma membrane H+-ATPase and 14-3-3 protein. Transient expression analysis using mesophyll cell protoplasts revealed that fuscicoccin, H+-ATPase activator induce a small change in signal intensity of the bio-sensor. Therefore, the function of these sensor protein is being evaluated in the transgenic plants, but it has not reached the practical level. In the future, we will re-examine the sensor protein and aim to visualize the activation state of plasma membrane H+-ATPases.

  5. オーキシン誘導性伸長生長におけるオーキシン受容と新規シグナル伝達機構の解析

    2014.4 - 2017.3

    科学研究費補助金  基盤研究(C)

    高橋宏二

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    Authorship:Principal investigator 

    オーキシン誘導性胚軸伸長とH+-ATPaseリン酸化を指標とした変異体スクリーニングとAuxin Binding Protein1の機能解析を行い、オーキシン酸成長の分子メカニズムと転写制御を介さない新規のオーキシンシグナル伝達機構を明らかにする。