Organization
Graduate School of Science Assistant Professor
Graduate School
Graduate School of Science
Undergraduate School
School of Science
Title
Assistant Professor
Contact information
External link
TAKAHASHI Koji
Degree 3
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Doctor of Agriculture （ 1997.3 Nagoya University ）
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修士（理学） （ 1993.3 東京大学 ）
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学士（理学） （ 1991.3 静岡大学 ）
Research Interests 1
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plant growth regulation
Research Areas 1
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Others / Others / Plant Physiology
Current Research Project and SDGs 2
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オーキシン誘導性伸長生長の分子生理学的研究
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Study on Growth Regulation and Cell Wall Proteins in Plant
Education 3
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Nagoya University Graduate School, Division of Agriculture
- 1996.3
Country： Japan
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The University of Tokyo Graduate School, Division of Science
1991.4 - 1993.3
Country： Japan
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Shizuoka University Faculty of Science Biology
1987.4 - 1991.3
Country： Japan
Professional Memberships 2
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The Japanese Society of Plant Physiologists
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The Botanical Society of Japan
Committee Memberships 3
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日本植物生理学会 運営委員会 広報幹事
2020.3 - 2022.3
Committee type：Academic society
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日本植物学会 第84回大会 実行委員会 オンライン発表担当代表
2020.9
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第60回 日本植物生理学会年会 大会実行委員会 会場担当代表
2019.3
Committee type：Academic society
Papers 38
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Discovery of a Plant 14-3-3 Inhibitor possessing Isoform Selectivity and in planta Activity. Reviewed
Kotaro Nishiyama, Yusuke Aihara, Takehiro Suzuki, Koji Takahashi, Toshinori Kinoshita, Naoshi Dohmae, Ayato Sato, Shinya Hagihara
Angewandte Chemie International Edition Vol. 63 ( 27 ) page： e202400218 2024.6
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Phosphorylation of plasma membrane H+-ATPase Thr881 participates in light-induced stomatal opening Reviewed
Yuki Hayashi, Kohei Fukatsu, Koji Takahashi, Satoru N. Kinoshita, Kyohei Kato, Taku Sakakibara, Keiko Kuwata & Toshinori Kinoshita
Nature Communications Vol. 15 page： 1194 2024.2
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Identification and improvement of isothiocyanate-based inhibitors on stomatal opening to act as drought tolerance-conferring agrochemicals Reviewed
Yusuke Aihara, Bumpei Maeda, Kanna Goto, Koji Takahashi, Mika Nomoto, Shigeo Toh, Wenxiu Ye, Yosuke Toda, Mami Uchida, Eri Asai, Yasuomi Tada, Kenichiro Itami, Ayato Sato, Kei Murakami* and Toshinori Kinoshita
Nature Communications Vol. 14 page： 2665 2023.5
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Tryptophan synthase ß subunit 1 affects stomatal phenotypes in Arabidopsis thaliana Reviewed
Midori N Soda, Yuki Hayashi, Koji Takahashi, Toshinori Kinoshita
Frontiers in Plant Science Vol. 13 page： 1011360 2022.11
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ABP1-TMK auxin perception mediates ultrafast global phosphorylation and auxin canalization. Reviewed International coauthorship
Jiří Friml, Michelle Gallei, Zuzana Gelová, Alexander Johnson, Ewa Mazur, Aline Monzer, Lesia Rodriguez, Mark Roosjen, Inge Verstraeten, Branka Živanović, Minxia Zou, Lukas Fiedler, Caterina Giannini, Peter Grones, Walter Kaufmann, Andre Kuhn, Madhumitha Narasimhan, Marek Randuch, Nikola Rydza, Koji Takahashi, Shutang Tan, Anastasia Teplova, Toshinori Kinoshita, Dolf Weijers, Hana Rakusova.
Nature Vol. 609 page： 575 - 581 2022.9
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Identification of stomatal-regulating molecules from de novo arylamine collection through aromatic C-H amination. Reviewed
Yosuke Toda, Gregory J.P. Perry, Shimpei Inoue, Eri Ito, Takahiro Kawakami, Mina R. Narouz, Koji Takahashi, Yusuke Aihara, Bumpei Maeda, Toshinori Kinoshita, Kenichiro Itami, Kei Murakami
Scientific Reports Vol. 12 page： 949 2022.1
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Identification of ABA-dependent phosphorylated bHLH transcription factors in guard cells of Vicia faba by mass spectrometry. Reviewed
Yuki Hayashi, Yohei Takahashi, Kohei Fukatsu, Yasuomi Tada, Koji Takahashi, Keiko Kuwata, Takamasa Suzuki, Toshinori Kinoshita
Frontiers in Plant Science Vol. 12 page： 735271 2021.12
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Cell surface and intracellular auxin signalling for H<SUP>+</SUP> fluxes in root growth
Li, LX; Verstraeten, I; Roosjen, M; Takahashi, K; Rodriguez, L; Merrin, J; Chen, J; Shabala, L; Smet, W; Ren, H; Vanneste, S; Shabala, S; De Rybel, B; Weijers, D; Kinoshita, T; Gray, WM; Friml, J
NATURE Vol. 599 ( 7884 ) page： 273 - + 2021.11
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TMK-based cell-surface auxin signalling activates cell-wall acidification
Lin, WW; Zhou, X; Tang, WX; Takahashi, K; Pan, X; Dai, JW; Ren, H; Zhu, XY; Pan, SQ; Zheng, HY; Gray, WM; Xu, TD; Kinoshita, T; Yang, ZB
NATURE Vol. 599 ( 7884 ) page： 278 - + 2021.11
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A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair Reviewed
Vol. 48 ( 18 ) 2020.10
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Pinstatic Acid Promotes Auxin Transport by Inhibiting PIN Internalization Reviewed
Oochi Akihiro, Hajny Jakub, Fukui Kosuke, Nakao Yukio, Gallei Michelle, Quareshy Mussa, Takahashi Koji, Kinoshita Toshinori, Harborough Sigurd Ramans, Kepinski Stefan, Kasahara Hiroyuki, Napier Richard, Friml Jiri, Hayashi Ken-ichiro
PLANT PHYSIOLOGY Vol. 180 ( 2 ) page： 1152 - 1165 2019.6
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Minami Anzu, Takahashi Koji, Inoue Shin-ichiro, Tada Yasuomi, Kinoshita Toshinori
PLANT AND CELL PHYSIOLOGY Vol. 60 ( 5 ) page： 935 - 944 2019.5
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A Super Strong Engineered Auxin-TIR1 Pair Reviewed
Yamada Ryotaro, Murai Keiichiro, Uchida Naoyuki, Takahashi Koji, Iwasaki Rie, Tada Yasuomi, Kinoshita Toshinori, Itami Kenichiro, Torii Keiko U., Hagihara Shinya
PLANT AND CELL PHYSIOLOGY Vol. 59 ( 8 ) page： 1538 - 1544 2018.8
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Rapid and reversible root growth inhibition by TIR1 auxin signalling Reviewed
Fendrych Matyas, Akhmanova Maria, Merrin Jack, Glanc Matous, Hagihara Shinya, Takahashi Koji, Uchida Naoyuki, Torii Keiko U., Friml Jiri
NATURE PLANTS Vol. 4 ( 7 ) page： 453 - 459 2018.7
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Chemical hijacking of auxin signaling with an engineered auxin-TIR1 pair. Reviewed
Naoyuki Uchida, Koji Takahashi, Rie Iwasaki, Ryotaro Yamada, Masahiko Yoshimura, Takaho A Endo, Seisuke Kimura, Hua Zhang, Mika Nomoto, Yasuomi Tada, Toshinori Kinoshita, Kenichiro Itami, Shinya Hagihara, Keiko U Torii
Nature Chemical Biology Vol. 14 ( 3 ) page： 299 - + 2018.3
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Auxin influx carrier AUX1 confers acid resistance for Arabidopsis root elongation through the regulation of plasma membrane H+-ATPase Reviewed
Shin-ichiro Inoue, Koji Takahashi, Hiromi Okumura-Noda, Toshinori Kinoshita
Plant and Cell Physiology Vol. 57 ( 10 ) page： 2194-2201 2016
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A flowering integrator, SOC1, affects stomatal opening in Arabidopsis thaliana. Reviewed
Kimura Y, Aoki S, Ando E, Kitatsuji A, Watanabe A, Ohnishi M, Takahashi K, Inoue S, Nakamichi N, Tamada Y, Kinoshita T
Plant & Cell Physiology Vol. 56 ( 4 ) page： 640-649 2015.4
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Abscisic acid suppresses hypocotyl elongation by dephosphorylating plasma membrane H+-ATPase in Arabidopsis thaliana Reviewed
Yuki Hayashi, Koji Takahashi, Shin-ichiro Inoue and Toshinori Kinoshita
Plant and Cell Physiology 2014
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Mg-chelatase I subunit 1 and Mg-protoporphyrin IX methyltransferase affect the stomatal aperture in Arabidopsis thaliana Reviewed
Masakazu Tomiyama, Shin-ichiro Inoue, Tomo Tsuzuki, Midori Soda, Sayuri Morimoto, Yukiko Okigaki, Takaya Ohishi, Nobuyoshi Mochizuki, Koji Takahashi, Toshinori Kinoshita
Journal of Plant Research Vol. 127 page： 553-563 2014
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Overexpression of the Mg-chelatase H subunit in guard cells confers drought tolerance via promotion of stomatal closure in Arabidopsis thaliana Reviewed
Tomo Tsuzuki, Koji Takahashi, Masakazu Tomiyama, Shin-ichiro Inoue and Toshinori Kinoshita
Frontiers in Plant Science 2013.10
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Evolutionary appearance of the plasma membrane H+-ATPase containing a penultimate threonine in the bryophyte Reviewed
Masaki Okumura, Koji Takahashi, Shin-ichiro Inoue, Toshinori Kinoshita
Plant Signaling & Behavior Vol. 7 ( 8 ) page： 1-4 2012.8
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Characterization of the plasma membrane H+-ATPase in the liverwort Marchantia polymorpha Reviewed
Masaki Okumura, Shin-ichiro Inoue, Koji Takahashi, Kimitsune Ishizaki, Takayuki Kohchi, Toshinori Kinoshita
Plant Physiology Vol. 159 page： 826-834 2012.6
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Auxin activates the plasma membrane H+-ATPase by phosphorylation during hypocotyl elongation in Arabidopsis thaliana Reviewed
Koji Takahashi, Ken-ichiro Hayashi, Toshinori Kinoshita
Plant Physiology Vol. 159 page： 632-641 2012.6
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Mg-chelatase H subunit affects ABA signaling in stomatal guard cells, but is not an ABA receptor in Arabidopsis thaliana. Reviewed
Tsuzuki T, Takahashi K, Inoue S, Okigaki Y, Tomiyama M, Hossain MA, Shimazaki K, Murata Y, Kinoshita T
Journal of Plant Research Vol. 124 page： 527-538 2011
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Immunohistochemical detection of blue light-induced phosphorylation of the plasma membrane H+-ATPase in stomatal guard cells. Reviewed
Hayashi M, Inoue S, Takahashi K, Kinoshita T
Plant and Cell Physiology Vol. 52 page： 1238-1248 2011
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Wall-yielding properties of cell walls from elongating cucumber hypocotyls in relation to the action of expansin Reviewed
Takahashi K, Hirata S, Kido N, and Katou K
Plant and Cell Physiology Vol. 47 ( 11 ) page： 1520-1529 2006
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The Role of wall Ca<SUP>2+</SUP> in the regulation of wall extensibility during the acid-induced extension of soybean hypocotyl cell walls Reviewed
Naofumi Ezaki, Nobuo Kido, Koji Takahashi and Kiyoshi Katou
Plant and Cell Physiology Vol. 46 ( 11 ) page： 1831-1838 2005
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Distribution of yieldin, a regulatory protein of the cell wall yield threshold, in etiolated cowpea seedlings. Reviewed
Okamoto-Nakazato A, Takahashi K, Katoh-Semba R, Katou K
Plant and Cell Physiology Vol. 42 ( 9 ) page： 952-958 2001
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Sugar-induced increase in cytosolic Ca<SUP>2+</SUP> in Arabidopsis thaliana whole plants. Reviewed
Furuichi T, Mori IC, Takahashi K, Muto S.
Plant and Cell Physiology Vol. 42 ( 10 ) page： 1149-1155 2001
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Molecular cloning of yieldins regulating the yield threshold of cowpea cell walls: cDNA cloning and characterization of recombinant yieldin Reviewed
Akane Okamoto-Nakazato, Koji Takahashi, Nobuo Kido, Katsushi Owaribe, and Kiyoshi Katou
Plant, Cell and Environment Vol. 23 ( 2 ) page： 155-164 2000
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A calcium-dependent protein kinase functions in wound healing in Ventricaria ventricosa (Chlorophyta) Reviewed
Koh-ichi Sugiyama, Izumi C. Mori, Koji Takahashi, Shoshi Muto and Ikuko Shihira-Ishikawa
Journal of Phycology Vol. 36 ( 6 ) page： 1145-1152 2000
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Salicylic acid induces extracellular superoxide generation follows by an increase in cytosolic calcium ion in tobacco suspension culture : the earliest events in salicylic acid signal transduction Reviewed
Tomonori Kawano, Nobuya Sahashi, Koji Takahashi, Nobuyuki Uozumi and Shoshi Muto
Plant and Cell Physiology Vol. 39 ( 7 ) page： 721-730 1998
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Calcium chelator and channel blockers supress the IAA-induced membrane hyperpolarization without inhibiting the following growth promotion in hypocotyl sections of Vigna unguiculata under xylem perfusion Reviewed
Koji Yuasa, Koji Takahashi and Kiyoshi Katou
Plant and Cell Physiology Vol. 39 ( 9 ) page： 978-986 1998
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Mastoparan induces an increase in cytosolic calcium ion concentration and subsequent activation of protein kinases in tobacco suspension culture cells Reviewed
Koji Takahashi, Minoru Isobe and Shoshi Muto
Biochimica et Biophysica Acta-Molecular Cell Research Vol. 1401 ( 3 ) page： 339-346 1998
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An increase in cytosolic calcium ion concentration precedes hypoosmotic shock-induced activation of protein kinases in tobacco suspension culture cells Reviewed
Koji Takahashi, Minoru Isobe and Shoshi Muto
FEBS Letters Vol. 401 ( 2-3 ) page： 202-206 1997
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Hypoosmotic shock induces increases in cytosolic Ca2+ in tobacco suspension-culture cells Reviewed
Koji Takahashi, Minoru Isobe, Marc R. Knight, Anthony J. Trewavas and Shoshi Muto
Plant Physiology Vol. 113 ( 2 ) page： 587-594 1997
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Purification and characterization of an algal (Dunaliella tertiolecta) protein cross-reacting with an anti-Gα-common antibody Reviewed
Koji Takahashi, Takashi Yuasa and Shoshi Muto
Physiologia Plantarum Vol. 94 ( 3 ) page： 486-490 1995
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Purification and characterization of a Ca<SUP>2+</SUP>-dependent protein kinase from the halotolerant green alga Dunaliella tertiolecta. Reviewed
Takashi Yuasa, Koji Takahashi and Shoshi Muto
Plant and Cell Physiology Vol. 36 ( 4 ) page： 699-708 1995
Books 2
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テイツ/ザイガー植物生理学・発生学 原著第６版 第3章 水と植物細胞、第4章 植物における水収支
木下俊則、高橋宏二（第3章、第4章）（ Role： Joint translator）
講談社サイエンティフィック 2017.2
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The regulation of plant cell expansion: Auxin-induced turgor-driven cell elongation. In "Molecular Cell Biology of the Growth and Differentiation of Plant Cells"
Koji Takahashi, Toshinori Kinoshita（ Role： Joint author）
CRC Press 2016
MISC 5
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オーキシン応答の自在操作を実現する人工ホルモン・受容体ペアの創成
高橋宏二、萩原伸也、鳥居啓子、打田直行
化学と生物 Vol. 57 ( 2 ) page： 80 - 87 2019
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Harnessing Synthetic Chemistry to Probe and Hijack Auxin Signaling
Keiko U. Torii, Naoyuki Uchida, Koji Takahashi, Shinya Hagihara
New Phytologist. Vol. 220 ( 2 ) page： 417 - 424 2018.8
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植物細胞壁の臨海降伏張力を制御するタンパク質yieldin オーキシンによる酸成長を支える新機能タンパク質
高橋宏二、中里（岡本）朱根、加藤潔
化学と生物 Vol. 39 ( 1 ) page： 9 - 10 2001
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エクオリンを用いた高等植物の細胞内カルシウムの測定
高橋宏二、森泉、武藤尚志
化学と生物 Vol. 36 ( 1 ) page： 66 - 70 1998
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浸透圧調節とプロテインキナーゼ
武藤尚志、湯浅高志、高橋宏二
現代化学 Vol. 増刊30 page： 146 - 151 1996
Presentations 15
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進化的視点からみた苔類ゼニゴケの細胞膜プロトンポンプ
奥村将樹、井上晋一郎、高橋宏二、石崎公庸、河内孝之、木下俊則
第76回日本植物学会
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気孔孔辺細胞に特異的に発現する遺伝子の探索
小屋翔太、小野奈津子、高橋宏二、木下俊則
第76回日本植物学会
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Auxin activates the plasma membrane H+-ATPase by phosphorylation of the penultimate threonine during hypocotyl elongation in Arabidopsis thaliana International conference
Takahashi K, Hayashi K, Kinoshita T
23rd International Conference on Arabidopsis Research
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Mg-キラターゼHサブユニットの孔辺細胞のABAシグナル伝達への関与
都築朋、高橋宏二、井上晋一郎、富山将和、村田芳行、島崎研一郎、木下俊則
第53回日本植物生理学会
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オーキシン誘導性伸長生長における細胞膜プロトンポンプの活性制御
高橋宏二、林謙一郎、木下俊則
第53回日本植物生理学会
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苔類ゼニゴケにおける細胞膜プロトンポンプの同定と活性化機構の解析
奥村将樹、井上晋一郎、高橋宏二、石崎公庸、河内孝之、木下俊則
第53回日本植物生理学会
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免疫組織学的手法による孔辺細胞細胞膜H+-ATPaseの青色光に依存したリン酸化の検出
林真妃、井上晋一郎、高橋宏二、木下俊則
第53回日本植物生理学会
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オーキシン誘導性伸長生長への細胞膜プロトンポンプの関与
高橋宏二、木下俊則
第75回日本植物学会
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苔類ゼニゴケにおける細胞膜プロトンポンプの探索および機能解析
奥村将樹、井上晋一郎、石崎公庸、河内孝之、高橋宏二、木下俊則
第75回日本植物学会
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青色光に依存した孔辺細胞細胞膜H+-ATPaseリン酸化の免疫組織学的検出を用いた気孔開閉シグナル伝達の解明
林真妃、井上晋一郎、高橋宏二、木下俊則
第75回日本植物学会
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苔類ゼニゴケにおける細胞膜プロトンポンプの構造および機能解析 International conference
奥村将樹、井上晋一郎、高橋宏二、石崎公庸、河内孝之、木下俊則
第52回日本植物生理学会
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オーキシンによるシロイヌナズナ胚軸伸長促進機構の解析
高橋宏二、木下俊則
第52回日本植物生理学会
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免疫組織染色による青色光に依存した孔辺細胞細胞膜H+-ATPaseのリン酸化の検出
林真妃、井上晋一郎、高橋宏二、木下俊則
第52回日本植物生理学会
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アブシジン酸非感受性の新奇気孔開度変異体rtl1の解析
都築 朋、 高橋宏二、 井上晋一郎、 沖垣友季子、 村田芳行、 島崎研一郎、木下俊則
第52回日本植物生理学会
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アブシジン酸非感受性の新奇気孔開度変異体rtl1の単離と表現型解析
都築 朋、沖垣友季子、高橋宏二、木下俊則
第74回日本植物学会
KAKENHI (Grants-in-Aid for Scientific Research) 4
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植物の細胞膜H+-ATPase活性制御機構に関わる新規因子の探索と機能解析
Grant number：24K09501 2024.4 - 2027.3
科学研究費助成事業 基盤研究(C)
高橋 宏二
Authorship：Principal investigator
Grant amount：\4550000 （ Direct Cost: \3500000 、 Indirect Cost：\1050000 ）
植物の細胞膜H+-ATPaseはリン酸化を介して活性制御される起電性プロトンポンプであり、細胞内外のpHの変動や恒常性維持および二次輸送体の活性調節を司り、篩部ローディング、根の溶質取込み、気孔開閉、細胞拡大など様々な生理反応を仲介する。本研究では、陸上進出の最初期に分岐したクレブソルミディウムと陸上に生息する顕花モデル植物シロイヌナズナを用いて、陸上植物に特有の細胞膜H+-ATPase活性化機構の解明を目指す。
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Modification of auxin receptors and mechanism of acid growth
Grant number：20K06685 2020.4 - 2023.3
Grants-in-Aid for Scientific Research Grant-in-Aid for Scientific Research (C)
Takahashi Koji
Authorship：Principal investigator
Grant amount：\4290000 （ Direct Cost: \3300000 、 Indirect Cost：\990000 ）
To artificially control the intracellular signal transduction of the phytohormone auxin, which mediates various physiological actions such as morphogenesis, growth regulation, and environmental response throughout the life cycle of plants, I have developed novel auxin research tools by modifying auxin receptor TIR1/AFB and the degron sequences of co-receptor Aux/IAA. In addition, I have clarified the mechanism of auxin-induced plant cell elongation (acid growth), which is a representative physiological action of auxin.
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Live-imaging of PM H+-ATPase activity
Grant number：17K07443 2017.4 - 2020.3
Takahashi Koji
Authorship：Principal investigator
Grant amount：\4940000 （ Direct Cost: \3800000 、 Indirect Cost：\1140000 ）
To visualize the plasma membrane H+-ATPase activity, I tried to produce the bio-image sensor using fluorescence resonance energy transfer (FRET) or circulary permutated fluorescent protein which can detect the bimolecular interaction between plasma membrane H+-ATPase and 14-3-3 protein. Transient expression analysis using mesophyll cell protoplasts revealed that fuscicoccin, H+-ATPase activator induce a small change in signal intensity of the bio-sensor. Therefore, the function of these sensor protein is being evaluated in the transgenic plants, but it has not reached the practical level. In the future, we will re-examine the sensor protein and aim to visualize the activation state of plasma membrane H+-ATPases.
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オーキシン誘導性伸長生長におけるオーキシン受容と新規シグナル伝達機構の解析
2014.4 - 2017.3
科学研究費補助金 基盤研究(C)
高橋宏二
Authorship：Principal investigator
オーキシン誘導性胚軸伸長とH+-ATPaseリン酸化を指標とした変異体スクリーニングとAuxin Binding Protein1の機能解析を行い、オーキシン酸成長の分子メカニズムと転写制御を介さない新規のオーキシンシグナル伝達機構を明らかにする。